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Phenotypic characterization of culture expanded rabbit limbal corneal keratocytes

Abd Ghafar N, Chua KH, Wan Ngah WZ, Che Hamzah J, Othman F, Abd Rahman R, et al.

Cell Tissue Bank, 2014 Mar;15(1):25-34.
PMID: 23292197 DOI: 10.1007/s10561-012-9360-y

The in vivo quiescent corneal stroma keratocytes need to be transformed to activated state in order to obtain sufficient number of cells either for monolayer evaluation or corneal stroma reconstruction. This study aimed to investigate the phenotypic characterization of corneal stromal cells during culture expansion from the limbal region of the cornea. Isolated corneal keratocytes from limbal tissue of New Zealand White Strain rabbits' corneas (n = 6) were culture expanded until three passages. Keratocytes morphology was examined daily with viability, growth rate, number of cell doubling and population doubling time were recorded at each passage. The expression of collagen type 1, aldehyde dehydrogenase (ALDH), lumican and alpha smooth muscle actin (α-SMA) were detected by RT-PCR. Immunocytochemistry was also used to detect ALDH, α-SMA, collagen type I and Cytokeratin-3 (CK3). Growth kinetic study revealed that the growth rate was low at the initial passage but increase to about two folds with concomitant reduction in population doubling time in later passages. Freshly isolated and cultured keratocytes expressed collagen type 1, ALDH and lumican but α-SMA expression was absent. However, α-SMA was expressed along with the other genes during culture expansion. Keratocytes at P1 expressed all the proteins except CK3. These results suggest that cultured keratocytes maintained most of the gene expression profile of native keratocytes while the emergence of α-SMA in serial passages showed a mix population of various phenotypes. The phenotypic characterization of monolayer keratocytes provides useful information before reconstruction of bioengineered tissue or in vitro pharmaceutical applications.

Matched MeSH terms: Bioengineering
100 Years of the Journal of Dental Research: A Bibliometric Analysis

Ahmad P, Alam MK, Jakubovics NS, Schwendicke F, Asif JA

J Dent Res, 2019 Dec;98(13):1425-1436.
PMID: 31746684 DOI: 10.1177/0022034519880544

Since its inception in 1919, the Journal of Dental Research has continually published high-quality articles that span the breadth of research topics relevant to dentistry, oral surgery, and medicine. As part of the journal's centennial celebration, we conducted an electronic search on Scopus to identify and analyze the top 100 most cited articles from 1919 to 2018. Since Scopus does not capture older citations, we conducted an additional analysis by Google Scholar to identify key articles published in the first 50 y of the journal. Based on Scopus, the articles were ranked in descending order per their citation counts. The citation counts of the 100 most cited articles varied from 262 to 1,503. The year in which the largest number of top 100 articles were published was 2004 (n = 6). Within the top 100, the majority of articles originated from the United States (n = 52). Research Reports-Biomaterials & Bioengineering was the most frequent category of cited articles (n = 35). There was no significant association between total citation count and time since publication (correlation coefficient = -0.051, P = 0.656). However, there was a significant negative association of citation density (correlation coefficient = -0.610, P < 0.01) with time since publication. Our analyses demonstrate the broad reach of the journal and the dynamics in citation patterns and research agenda over its 100-y history. There is considerable evidence of the high variance in research output, when measured via citations, across the globe. Moreover, it remains unclear how patients' priorities and dental health care needs are aligned with the perceived influence of single research pieces identified by our search. Our findings may help to inspire future research in tackling these inequalities and highlight the need for conceptualizing research priorities.

Matched MeSH terms: Bioengineering
Fulltext Functional diversity of biocatalysts: whether to bioprospect or bioengineer?

Benbelgacem, Farah Fadwa, Bellag, Oualid Abdelkader, Soroodi, Fatemeh, Abdul Aziz Ahmad, Hamzah Mohd Salleh, Noorbatcha, Ibrahim Ali

Biological and Natural Resources Engineering Journal, 2019;2(1):64-84.
MyJurnal

Biocatalyst should have sufficient and efficient activity for the intended
biotechnological application. In the quest for novel biocatalyst, there is a need to have a
genetic diversity either by finding it within the astronomically large number of possible
candidates or to obtain it by bioengineering an existing gene supported by various
bioinformatic and molecular engineering tools. Nowadays, it is well-known that a huge
number of microorganisms is unculturable and poses great challenges to access biocatalysts
from these microbes. Metagenomics is one of the methods widely applied to reach out
maximum possible variants to “bioprospect” biocatalysts. On the other hand, other approaches
are available to bioengineer enzymes by modifying the DNA sequence precisely based on the
structure and the function information of the protein in the case of rational design, or by a
brave creation of anarchic mutations of the DNA sequence with directed evolution method. In
this regard, both approaches, whether to bioprospect or to bioengineer biocatalysts have
advantages and disadvantages which will be discussed in this paper.KEY WORDS: Sugar
industry wastewater; aluminium sulphate; primary treatment, ferric chloride; polyaluminium
chloride

Matched MeSH terms: Bioengineering
Bioengineering of microbial transglutaminase for biomedical applications

Chan SK, Lim TS

Appl Microbiol Biotechnol, 2019 Apr;103(7):2973-2984.
PMID: 30805670 DOI: 10.1007/s00253-019-09669-3

Microbial transglutaminase (mTGase) is commonly known in the food industry as meat glue due to its incredible ability to "glue" meat proteins together. Aside from being widely exploited in the meat processing industries, mTGase is also widely applied in other food and textile industries by catalysing the formation of isopeptide bonds between peptides or protein substrates. The advancement of technology has opened up new avenues for mTGase in the field of biomedical engineering. Efforts have been made to study the structural properties of mTGase in order to gain an in-depth understanding of the structure-function relationship. This review highlights the developments in mTGase engineering together with its role in biomedical applications including biomaterial fabrication for tissue engineering and biotherapeutics.

Matched MeSH terms: Bioengineering/methods*
Biorefineries of carbon dioxide: From carbon capture and storage (CCS) to bioenergies production

Cheah WY, Ling TC, Juan JC, Lee DJ, Chang JS, Show PL

Bioresour Technol, 2016 Sep;215:346-56.
PMID: 27090405 DOI: 10.1016/j.biortech.2016.04.019

Greenhouse gas emissions have several adverse environmental effects, like pollution and climate change. Currently applied carbon capture and storage (CCS) methods are not cost effective and have not been proven safe for long term sequestration. Another attractive approach is CO2 valorization, whereby CO2 can be captured in the form of biomass via photosynthesis and is subsequently converted into various form of bioenergy. This article summarizes the current carbon sequestration and utilization technologies, while emphasizing the value of bioconversion of CO2. In particular, CO2 sequestration by terrestrial plants, microalgae and other microorganisms are discussed. Prospects and challenges for CO2 conversion are addressed. The aim of this review is to provide comprehensive knowledge and updated information on the current advances in biological CO2 sequestration and valorization, which are essential if this approach is to achieve environmental sustainability and economic feasibility.

Matched MeSH terms: Bioengineering/trends
Bioengineering of the Plant Culture of Capsicum frutescens with Vanillin Synthase Gene for the Production of Vanillin

Chee MJ, Lycett GW, Khoo TJ, Chin CF

Mol Biotechnol, 2017 Jan;59(1):1-8.
PMID: 27826796 DOI: 10.1007/s12033-016-9986-2

Production of vanillin by bioengineering has gained popularity due to consumer demand toward vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a plant system. The VpVAN enzyme had been shown to directly convert ferulic acid and its glucoside into vanillin and its glucoside, respectively. As the ferulic acid precursor and vanillin were found to be the intermediates in the phenylpropanoid biosynthetic pathway of Capsicum species, this work serves as a proof-of-concept for vanillin production using Capsicum frutescens (C. frutescens or hot chili pepper). The cells of C. frutescens were genetically transformed with a codon optimized VpVAN gene via biolistics. Transformed explants were selected and regenerated into callus. Successful integration of the gene cassette into the plant genome was confirmed by polymerase chain reaction. High-performance liquid chromatography was used to quantify the phenolic compounds detected in the callus tissues. The vanillin content of transformed calli was 0.057% compared to 0.0003% in untransformed calli.

Matched MeSH terms: Bioengineering/methods
Safety and efficacy of the COMBO bio-engineered stent in an all-comer PCI cohort: 1-Year final clinical outcomes from the MASCOT post-marketing registry

Colombo A, Chandrasekhar J, Aquino M, Ong TK, Sartori S, Baber U, et al.

Int J Cardiol, 2019 05 15;283:67-72.
PMID: 30826192 DOI: 10.1016/j.ijcard.2019.01.053

BACKGROUND: The COMBO stent (OrbusNeich Medical, Ft. Lauderdale, Florida) is a new-generation bio-engineered drug eluting stent, combining an abluminal coating of a bioabsorbable polymer matrix for sustained release of sirolimus and luminal anti-CD34 coating for endothelial progenitor cell capture and rapid endothelialization.
METHODS: The Multinational Abluminal Sirolimus Coated BiO-Engineered StenT (MASCOT) registry was a prospective post-marketing study conducted from June 2014-May 2017 across 60 centers globally. Patients were eligible if COMBO stent implantation was attempted, and they received dual antiplatelet therapy (DAPT) per local guidelines. Follow-up was conducted by trained research staff at 1, 6 and 12 months by phone or clinic visit to capture clinical events and DAPT cessation events. The primary endpoint was 1-year target lesion failure (TLF), composite of cardiac death, non-fatal myocardial infarction not clearly attributable to a non-target vessel, or ischemia-driven target lesion revascularization.
RESULTS: A total of 2614 patients were enrolled over the study period with 96.7% completion of 1-year follow-up. The mean age of enrolled patients was 62.9 ± 11.2 years and 23.0% were female. Diabetes mellitus was present at baseline in 33.5%. A total of 56.1% patients underwent PCI for acute coronary syndrome (ACS). The 1-year primary endpoint of TLF occurred in 3.4% patients (n = 88). Definite stent thrombosis occurred in 0.5% patients (n = 12).
CONCLUSION: The MASCOT post marketing registry provides comprehensive safety and efficacy outcomes following contemporary PCI using the novel COMBO stent in an all-comer population. This platform is associated with low rates of 1-year TLF and ST. CLINICALTRIALS.
GOV IDENTIFIER: NCT02183454.

Matched MeSH terms: Bioengineering/methods
Fulltext Present practices and emerging opportunities in bioengineering for slope stabilization in Malaysia: An overview

Dorairaj D, Osman N

PeerJ, 2021;9:e10477.
PMID: 33520435 DOI: 10.7717/peerj.10477

Population increase and the demand for infrastructure development such as construction of highways and road widening are intangible, leading up to mass land clearing. As flat terrains become scarce, infrastructure expansions have moved on to hilly terrains, cutting through slopes and forests. Unvegetated or bare slopes are prone to erosion due to the lack of or insufficient surface cover. The combination of exposed slope, uncontrolled slope management practices, poor slope planning and high rainfall as in Malaysia could steer towards slope failures which then results in landslides under acute situation. Moreover, due to the tropical weather, the soils undergo intense chemical weathering and leaching that elevates soil erosion and surface runoff. Mitigation measures are vital to address slope failures as they lead to economic loss and loss of lives. Since there is minimal or limited information and investigations on slope stabilization methods in Malaysia, this review deciphers into the current slope management practices such as geotextiles, brush layering, live poles, rock buttress and concrete structures. However, these methods have their drawbacks. Thus, as a way forward, we highlight the potential application of soil bioengineering methods especially on the use of whole plants. Here, we discuss the general attributions of a plant in slope stabilization including its mechanical, hydrological and hydraulic effects. Subsequently, we focus on species selection, and engineering properties of vegetation especially rooting structures and architecture. Finally, the review will dissect and assess the ecological principles for vegetation establishment with an emphasis on adopting the mix-culture approach as a slope failure mitigation measure. Nevertheless, the use of soil bioengineering is limited to low to moderate risk slopes only, while in high-risk slopes, the use of traditional engineering measure is deemed more appropriate and remain to be the solution for slope stabilization.

Matched MeSH terms: Bioengineering
Fulltext Mesenchymal Stem Cell-Derived Extracellular Vesicles: Regenerative Potential and Challenges

Fuloria S, Subramaniyan V, Dahiya R, Dahiya S, Sudhakar K, Kumari U, et al.

Biology (Basel), 2021 Feb 25;10(3).
PMID: 33668707 DOI: 10.3390/biology10030172

Evidence suggests that stem cells exert regenerative potential via the release of extracellular vesicles. Mesenchymal stem cell extracellular vesicles (MSCEVs) offer therapeutic benefits for various pathophysiological ailments by restoring tissues. Facts suggest that MSCEV action can be potentiated by modifying the mesenchymal stem cells culturing methodology and bioengineering EVs. Limited clinical trials of MSCEVs have questioned their superiority, culturing quality, production scale-up and isolation, and administration format. Translation of preclinically successful MSCEVs into a clinical platform requires paying attention to several critical matters, such as the production technique, quantification/characterization, pharmacokinetics/targeting/transfer to the target site, and the safety profile. Keeping these issues as a priority, the present review was designed to highlight the challenges in translating preclinical MSCEV research into clinical platforms and provide evidence for the regenerative potential of MSCEVs in various conditions of the liver, kidney, heart, nervous system, bone, muscle, cartilage, and other organs/tissues.

Matched MeSH terms: Bioengineering
Effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the Pseudomonas cepacia lipase immobilized on sol-gel support

Ganasen P, Khan MR, Kalam MA, Mahmud MS

Bioprocess Biosyst Eng, 2014 Nov;37(11):2353-9.
PMID: 24879090 DOI: 10.1007/s00449-014-1213-6

This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250-750 nm. The reaction follows Michaelis-Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver-Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.

Matched MeSH terms: Bioengineering
Biodegradation and metabolite transformation of pyrene by basidiomycetes fungal isolate Armillaria sp. F022

Hadibarata T, Kristanti RA

Bioprocess Biosyst Eng, 2013 Apr;36(4):461-8.
PMID: 22893180 DOI: 10.1007/s00449-012-0803-4

Armillaria sp. F022 is a white-rot fungus isolated from a tropical rain forest in Indonesia that is capable of utilizing pyrene as a source of carbon and energy. Enzymes production during the degradation process by Armillaria sp. F022 was certainly related to the increase in biomass. In the first week after incubation, the growth rate rapidly increased, but enzyme production decreased. After 7 days of incubation, rapid growth was observed, whereas, the enzymes were produced only after a good amount of biomass was generated. About 63 % of pyrene underwent biodegradation when incubated with this fungus in a liquid medium on a rotary shaker (120 rpm, 25 °C) for 30 days; during this period, pyrene was transformed to five stable metabolic products. These metabolites were extracted in ethyl acetate, isolated by column chromatography, and then identified using thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). 1-Hydroxypyrene was directly identified by GC-MS, while 4-phenanthroic acid, 1-hydroxy-2-naphthoic acid, phthalic acid, and protocatechuic acid were identified to be present in their derivatized forms (methylated forms and silylated forms). Protocatechuic acid was the end product of pyrene degradation by Armillaria sp. F022. Dynamic profiles of two key enzymes, namely laccase and 1,2-dioxygenase, were revealed during the degradation process, and the results indicated the presence of a complicated mechanism in the regulation of pyrene-degrading enzymes. In conclusion, Armillaria sp. F022 is a white-rot fungus with potential for application in the degradation of polycyclic aromatic hydrocarbons such as pyrene in the environment.

Matched MeSH terms: Bioengineering
Fulltext An innovations-based noise cancelling technique on inverse kepstrum whitening filter and adaptive FIR filter in beamforming structure

Jeong J

Sensors (Basel), 2011;11(7):6816-41.
PMID: 22163987 DOI: 10.3390/s110706816

This paper presents an acoustic noise cancelling technique using an inverse kepstrum system as an innovations-based whitening application for an adaptive finite impulse response (FIR) filter in beamforming structure. The inverse kepstrum method uses an innovations-whitened form from one acoustic path transfer function between a reference microphone sensor and a noise source so that the rear-end reference signal will then be a whitened sequence to a cascaded adaptive FIR filter in the beamforming structure. By using an inverse kepstrum filter as a whitening filter with the use of a delay filter, the cascaded adaptive FIR filter estimates only the numerator of the polynomial part from the ratio of overall combined transfer functions. The test results have shown that the adaptive FIR filter is more effective in beamforming structure than an adaptive noise cancelling (ANC) structure in terms of signal distortion in the desired signal and noise reduction in noise with nonminimum phase components. In addition, the inverse kepstrum method shows almost the same convergence level in estimate of noise statistics with the use of a smaller amount of adaptive FIR filter weights than the kepstrum method, hence it could provide better computational simplicity in processing. Furthermore, the rear-end inverse kepstrum method in beamforming structure has shown less signal distortion in the desired signal than the front-end kepstrum method and the front-end inverse kepstrum method in beamforming structure.

Matched MeSH terms: Bioengineering/methods*
Advancements of microalgal upstream technologies: Bioengineering and application aspects in the paradigm of circular bioeconomy

Leong WH, Rawindran H, Ameen F, Alam MM, Chai YH, Ho YC, et al.

Chemosphere, 2023 Oct;339:139699.
PMID: 37532206 DOI: 10.1016/j.chemosphere.2023.139699

Sustainable energy transition has brought the attention towards microalgae utilization as potential feedstock due to its tremendous capabilities over its predecessors for generating more energy with reduced carbon footprint. However, the commercialization of microalgae feedstock remains debatable due to the various factors and considerations taken into scaling-up the conventional microalgal upstream processes. This review provides a state-of-the-art assessment over the recent developments of available and existing microalgal upstream cultivation systems catered for maximum biomass production. The key growth parameters and main cultivation modes necessary for optimized microalgal growth conditions along with the fundamental aspects were also reviewed and evaluated comprehensively. In addition, the advancements and strategies towards potential scale-up of the microalgal cultivation technologies were highlighted to provide insights for further development into the upstream processes aimed at sustainable circular bioeconomy.

Matched MeSH terms: Bioengineering
Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Lew TTS, Wong MH, Kwak SY, Sinclair R, Koman VB, Strano MS

Small, 2018 Nov;14(44):e1802086.
PMID: 30191658 DOI: 10.1002/smll.201802086

The ability to control the subcellular localization of nanoparticles within living plants offers unique advantages for targeted biomolecule delivery and enables important applications in plant bioengineering. However, the mechanism of nanoparticle transport past plant biological membranes is poorly understood. Here, a mechanistic study of nanoparticle cellular uptake into plant protoplasts is presented. An experimentally validated mathematical model of lipid exchange envelope penetration mechanism for protoplasts, which predicts that the subcellular distribution of nanoparticles in plant cells is dictated by the particle size and the magnitude of the zeta potential, is advanced. The mechanism is completely generic, describing nanoparticles ranging from quantum dots, gold and silica nanoparticles, nanoceria, and single-walled carbon nanotubes (SWNTs). In addition, the use of imaging flow cytometry to investigate the influence of protoplasts' morphological characteristics on nanoparticle uptake efficiency is demonstrated. Using DNA-wrapped SWNTs as model nanoparticles, it is found that glycerolipids, the predominant lipids in chloroplast membranes, exhibit stronger lipid-nanoparticle interaction than phospholipids, the major constituent in protoplast membrane. This work can guide the rational design of nanoparticles for targeted delivery into specific compartments within plant cells without the use of chemical or mechanical aid, potentially enabling various plant engineering applications.

Matched MeSH terms: Bioengineering
Fulltext Characterization and safety assessment of bioengineered limbal epithelium

Lim MN, Umapathy T, Baharuddin PJ, Zubaidah Z

Med J Malaysia, 2011 Oct;66(4):335-41.
PMID: 22299553 MyJurnal

Transplantation of cultivated limbal epithelium on substrates such as amniotic membrane is an established treatment for severe ocular surface disease with limbal stem cell deficiency. In this study, we adapted an established method to generate sheets of limbal epithelium on amniotic membrane and characterized the cells contained in these sheets and tested them for safety with regard to microbial contamination. Human limbal biopsies were cultivated on denuded amniotic membranes. After three weeks of culture, the phenotypes of cultivated cells were analyzed by immunohistochemistry and real-time RT-PCR for the expression of a panel of specific markers. Cultivated limbal epithelial cell sheets were also analyzed by scanning (SEM) and transmission (TEM) electron microscopy. Sterility tests and mycoplasma assays were conducted for the safety of product. A confluent layer of polygonal cells was formed in 2 weeks and 1-3 stratified layer of cells were observed after three weeks of culture. Cultivated cells were positive for p63, K3, K19, and involucrin but negative for K14, integrin alpha9 and ABCG2 when analyzed by immunohistochemistry. Expression of molecular markers was detectable with real-time RT-PCR. SEM showed multilayer of flat squamous polygonal epithelial cells. Desmosomal and hemidesmosomal attachments were evident. Our study showed that cultivated limbal epithelium consists of limbal progenitors as well as differentiated corneal epithelial cells. SEM and TEM analysis showed cultivated cells demonstrated typical features of corneal epithelium. The risk of contamination is low and can be prevented by culturing the cells in a clean room facility complying to Good Manufacturing Practice standard.

Matched MeSH terms: Bioengineering*
Bioengineered silver nanoparticles capped with bovine serum albumin and its anticancer and apoptotic activity against breast, bone and intestinal colon cancer cell lines

Majeed S, Aripin FHB, Shoeb NSB, Danish M, Ibrahim MNM, Hashim R

Mater Sci Eng C Mater Biol Appl, 2019 Sep;102:254-263.
PMID: 31146998 DOI: 10.1016/j.msec.2019.04.041

The aim of the current study was to biosynthesize the silver nanoparticles (AgNPs) from the bacterial strain of Bacillus cereus (ATCC 14579) extracellularly. When bacterial extract was challenged with 1 mM silver nitrate (AgNO3) the color of the extract changed into brown confirms the formation of nanoparticles. These nanoparticles were capped with bovine serum albumin (BSA). UV- visible spectroscopy showed the absorption peak at 420 nm indicates the formation of AgNPs. Fourier Infra -red (FTIR) attenuated total reflection (ATR) spectroscopy showed amide and amine group associated with AgNPs that stabilizes the nanoparticles. Energy dispersive x-ray spectroscopy (EDX) showed a strong peak of silver confirms the presence of silver. Thermo gravimetric analysis (TGA) analysis was used to determine the protein degradation showed less protein degradation at higher temperature confirms the stability of nanoparticles. Transmission electron microscopy (TEM) showed the AgNPs are well dispersed and spherical, and 5.37 nm to 17.19 whereas albumin coated nanoparticles are size ranges from 11.26 nm to 23.85 nm. The anticancer effect of capped AgNPs (cAgNPs) showed the IC50 value against breast cancer MCF-7 at 80 μg/mL, intestinal colon cancer HCT- 116 60 μg/mL, and bone cancer osteosarcoma MG-63 cell line80 μg/mL while against normal fibroblast cells 3T3 cells showed the IC50 value at 140 μg/mL. Lactate dehydrogenase assay (LDH) showed higher toxicity on MCF-7, HCT-116, and MG-63 cells. The apoptotic study clearly showed the blebbing of membrane, chromatin condensation due to the production of reactive oxygen species (ROS) by ethidium bromide and acridine orange dual staining method. The DNA analysis showed the complete fragmentation of the DNA of treated cells when compared with control cells.

Matched MeSH terms: Bioengineering*
Applications of deep eutectic solvents in biotechnology and bioengineering-Promises and challenges

Mbous YP, Hayyan M, Hayyan A, Wong WF, Hashim MA, Looi CY

Biotechnol Adv, 2017 Mar-Apr;35(2):105-134.
PMID: 27923764 DOI: 10.1016/j.biotechadv.2016.11.006

Deep eutectic solvents (DESs) have been touted recently as potential alternatives to ionic liquids (ILs). Although they possess core characteristics that are similar to those of ILs (e.g., low volatility, non-flammability, low melting points, low vapor pressure, dipolar nature, chemical and thermal stability, high solubility, and tuneability), DESs are superior in terms of the availability of raw materials, the ease of storage and synthesis, and the low cost of their starting materials. As such, they have become the subject of intensive research in various sectors, notably the chemical, electrochemical, and biological sectors. To date, the applications of DESs have shown great promise, especially in the medical and biotechnological fields. In spite of these various achievements, the safety concern for these mixtures must be sufficiently addressed. Indeed, in order to exploit the vast array of opportunities that DESs offer to the biological industry, first, they must be established as safe mixtures. Hence, the biotechnological applications of DESs only can be implemented if they are proven to have negligible or low toxicity profiles. This review is the first of its kind, and it discusses two current aspects of DES-based research. First, it describes the properties of these mixtures with ample focus on their toxicity profiles. Second, it provides an overview of the breakthroughs that have occurred and the foreseeable prospects of the use of DESs in various biotechnological and biological applications.

Matched MeSH terms: Bioengineering*
Production of 3-Oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic Acid in the Fungus Aspergillus oryzae: A Step Towards Heterologous Production of Pyrethrins in Fungi

Mohamed ME, Pahirulzaman KA, Lazarus CM

Mol Biotechnol, 2016 Mar;58(3):172-8.
PMID: 26718544 DOI: 10.1007/s12033-015-9911-0

Pyrethrins are natural insecticides, which accumulate to high concentrations in pyrethrum (Chrysanthemum cinerariaefolium) flowers. Synthetic pyrethroids are more stable, more efficacious and cheaper, but contemporary requirements for safe and environmentally friendly pesticides encourage a return to the use of natural pyrethrins, and this would be favoured by development of an efficient route to their production by microbial fermentation. The biosynthesis of pyrethrins involves ester linkage between an acid moiety (chrysanthemoyl or pyrethroyl, synthesised via the mevalonic acid pathway from glucose), and an alcohol (pyrethrolone). Pyrethrolone is generated from 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid, which originates from α-linolenic acid via the jasmonic acid biosynthetic cascade. The first four genes in this cascade, encoding lipoxygenase 2, allene-oxide synthase, allene-oxide cyclase 2 and 12-oxophytodienoic acid reductase 3, were amplified from an Arabidopsis thaliana cDNA library, cloned in a purpose-built fungal multigene expression vector and expressed in Aspergillus oryzae. HPLC-MS analysis of the transgenic fungus homogenate gave good evidence for the presence of 3-oxo-2-(2'-pentenyl)-cyclopentane-1-octanoic acid.

Matched MeSH terms: Bioengineering
Fulltext A Systematic Analysis of Additive Manufacturing Techniques in the Bioengineering of In Vitro Cardiovascular Models

Mohanadas HP, Nair V, Doctor AA, Faudzi AAM, Tucker N, Ismail AF, et al.

Ann Biomed Eng, 2023 Nov;51(11):2365-2383.
PMID: 37466879 DOI: 10.1007/s10439-023-03322-x

Additive Manufacturing is noted for ease of product customization and short production run cost-effectiveness. As our global population approaches 8 billion, additive manufacturing has a future in maintaining and improving average human life expectancy for the same reasons that it has advantaged general manufacturing. In recent years, additive manufacturing has been applied to tissue engineering, regenerative medicine, and drug delivery. Additive Manufacturing combined with tissue engineering and biocompatibility studies offers future opportunities for various complex cardiovascular implants and surgeries. This paper is a comprehensive overview of current technological advancements in additive manufacturing with potential for cardiovascular application. The current limitations and prospects of the technology for cardiovascular applications are explored and evaluated.

Matched MeSH terms: Bioengineering*
Polyunsaturated fatty acids in marine bacteria and strategies to enhance their production

Moi IM, Leow ATC, Ali MSM, Rahman RNZRA, Salleh AB, Sabri S

Appl Microbiol Biotechnol, 2018 Jul;102(14):5811-5826.
PMID: 29749565 DOI: 10.1007/s00253-018-9063-9

Polyunsaturated fatty acids (PUFAs) play an important role in human diet. Despite the wide-ranging importance and benefits from heart health to brain functions, humans and mammals cannot synthesize PUFAs de novo. The primary sources of PUFA are fish and plants. Due to the increasing concerns associated with food security as well as issues of environmental contaminants in fish oil, there has been considerable interest in the production of polyunsaturated fatty acids from alternative resources which are more sustainable, safer, and economical. For instance, marine bacteria, particularly the genus of Shewanella, Photobacterium, Colwellia, Moritella, Psychromonas, Vibrio, and Alteromonas, are found to be one among the major microbial producers of polyunsaturated fatty acids. Recent developments in the area with a focus on the production of polyunsaturated fatty acids from marine bacteria as well as the metabolic engineering strategies for the improvement of PUFA production are discussed.

Matched MeSH terms: Bioengineering

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