Displaying publications 1 - 20 of 133 in total

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  1. Lim SL, Ishak Ahmad, Azwan Mat Lazim
    Sains Malaysiana, 2015;44:779-785.
    The purpose of this study was to produce a novel pH sensitive hydrogel with superior thermal stability, composed of
    poly(acrylic acid) (PAA) and cellulose nanocrystal (CNC). CNC was extracted from kenaf fiber through a series of alkali
    and bleaching treatments followed by acid hydrolysis. PAA was then subjected to chemical cross-linking using the crosslinking
    agent (N,N-methylenebisacrylamide) in CNC suspension. The mixture was casted onto petri dish to obtain disc
    shape hydrogel. PAA/cellulose hydrogel with the same composition ratio were also prepared as control. The effect of
    reaction conditions such as the ratio of PAA and CNC on the swelling behavior of the hydrogel obtained towards pH
    was studied. The obtained hydrogel was further subjected to different tests such as thermogravimetric analysis (TGA) to
    study the thermal behavior, Fourier transform infrared for functional group identification and swelling test for swelling
    behavior at different pH. The cross-linking of PAA was verified with FTIR with the absence of C=C double bond. In TGA
    test, PAA/CNC hydrogel showed significantly higher thermal stability compared with pure PAA hydrogel. The hydrogel
    obtained showed excellent pH sensitivity and experienced maximum swelling at pH7. The PAA/CNC hydrogel can be
    developed further as drug carrier
    Matched MeSH terms: Hibiscus
  2. Abdalla A. Ab. Rashdi, Mohd Sapuan Salit, Khalina Abdan, Megat Mohamad Hamdan Megat
    MyJurnal
    Fibre reinforced composites have gained use in a variety of applications. The performances of these composites may suffer when the material is exposed to adverse environments for a long period of time. Kenaf fibre reinforced unsaturated polyester composites were subjected to water immersion tests in order to study the effects of water absorption on the mechanical properties. Composites specimens containing (10%, 20%, and 30%) weight percentages of fibre were prepared. Water absorption tests were conducted by immersing these specimens in a distilled water bath at 25oC for four months. The tensile properties of the specimens immersed in water were evaluated and compared with the dry composite specimens. A decrease in the tensile properties of the composites was demonstrated, indicating a great loss in the mechanical properties of the water-saturated samples compared to the dry samples. The percentage of moisture uptake was also increased as the percentage of the fibre weight increased due to the high cellulose content. The water absorption pattern of these composites was found to follow the Fickian behaviour.
    Matched MeSH terms: Hibiscus
  3. Abu-Bakar NB, Makahleh A, Saad B
    J Sep Sci, 2016 Mar;39(5):947-55.
    PMID: 26718308 DOI: 10.1002/jssc.201501109
    A novel microextraction method based on vortex- and CO2 -assisted liquid-liquid microextraction with salt addition for the isolation of furanic compounds (5-hydroxymethyl-2-furaldehyde, 5-methyl-2-furaldehyde, 2-furaldehyde, 3-furaldehyde, 2-furoic and 3-furoic acids) was developed. Purging the sample with CO2 was applied after vortexing to enhance the phase separation and mass transfer of the analytes. The optimum extraction conditions were: extraction solvent (volume), propyl acetate (125 μL); sample pH, 2.4; vortexing time, 45 s; salt concentration, 25% w/v and purging time, 5 min. The analytes were separated using an ODS Hypersil C18 column (250×4.6 mm i.d, 5 μm) under gradient flow. The proposed method showed good linearities (r(2) >0.999), low detection limits (0.08-1.9 μg/L) and good recoveries (80.7-122%). The validated method was successfully applied for the determination of the furanic compounds in concentrated juice (mango, date, orange, pomegranate, roselle, mangosteen and soursop) and dried fruit (prune, date and apricot paste) samples.
    Matched MeSH terms: Hibiscus
  4. Ismail AS, Jawaid M, Naveen J
    Materials (Basel), 2019 Jun 28;12(13).
    PMID: 31261821 DOI: 10.3390/ma12132094
    This study aims to investigate the void content, tensile, vibration and acoustic properties of kenaf/bamboo fiber reinforced epoxy hybrid composites. The composites were made using the hand lay-up method. The weight ratios of kenaf/bamboo were 30:70, 50:50 and 70:30. Further, kenaf and bamboo composites were fabricated for the purpose of comparison. The hybridization of woven kenaf/bamboo reduced the void content. The void contents of hybrid composites were almost similar. An enhancement in elongation at break, tensile strength and modulus of hybrid composites was observed until a kenaf/bamboo ratio of 50:50. Kenaf/bamboo (50:50) hybrid composite displays the highest elongation at break, tensile strength and modulus compared to the other hybrid composites which are 2.42 mm, 55.18 MPa and 5.15 GPa, respectively. On the other hand, the highest natural frequency and damping factors were observed for Bamboo/Kenaf (30:70) hybrid composites. The sound absorption coefficient of composites were measured in two conditions: without air gap and with air gap (10, 20, 30 mm). The sound absorption coefficient for testing without air gap was less than 0.5. Introducing an air gap improved the sound absorption coefficient of all composites. Hence, hybrid kenaf/bamboo composites exhibited less void content, as well as improved tensile, vibration and acoustic properties.
    Matched MeSH terms: Hibiscus
  5. Nurul Izzah Ahmad, Aminah Abdullah, Md Pauzi Abdullah, Lee, Yook Heng, Wan Rozita Wan Mahiyuddin, Siti Fatimah Daud, et al.
    MyJurnal
    A survey was conducted to investigate the level of consumption of ‘ulam’ in Selangor State among 252 adults (> 17 years) (male 28.6%, female 71.4%) of major ethnics (Malays-51.6%; Chinese-30.5%; Indians-17.5%) with the mean age of 42.7 ± 13.9 years. Consumption data were collected using 24 hours duplicate samples together with questionnaire on perceptions of ‘ulam.’ Results showed that ‘ulam’ was preferred by majority of the subjects (82.1%), especially amongst Malays (92.3%). A total of 52% of the subjects consumed partially or boiled ‘ulam.’ Factors that affect their preferences on ‘ulam’ were the perception of therapeutic effects of the ‘ulam’ towards health, its good taste and unique
    aroma. The most consumed ‘ulam’ were cucumber (Cucumis sativus) (60.6%) ‘kacang botol’ (Psophocarpus tetragonolobus) (33%), Indian pennywort (Hydrocotyle asiatica) (31.5%), lettuce (Lactuca sativa) (27.6%), ‘petai’ (Parkia speciosa) (29%) and ‘ulam raja’ (Cosmos caudatus) (21.9%). The most preferred partially or boiled ‘ulam’
    were tapioca shoot (Manihot esculenta) (31.5%), ocra (Hibiscus esculentus) (12.5%) and ‘jantung pisang’ (Musa sapientum) (20.1%). There was no significant difference (P > 0.05) amongst the three different ethnic groups on the consumption of ‘ulam’ and the median for total intake per day was within the range of 30-39 g/day. Ulam is a potential
    source for increasing vegetable consumption to meet recommendation by World Health Organization (WHO), which is 400 g per day.
    Keywords: Adults; perception; ‘ulam;’ Selangor State
    Matched MeSH terms: Hibiscus
  6. Kamarudin SH, Abdullah LC, Aung MM, Ratnam CT
    Polymers (Basel), 2020 Nov 06;12(11).
    PMID: 33171889 DOI: 10.3390/polym12112604
    New environmentally friendly plasticized poly(lactic acid) (PLA) kenaf biocomposites were obtained through a melt blending process from a combination of epoxidized jatropha oil, a type of nonedible vegetable oil material, and renewable plasticizer. The main objective of this study is to investigate the effect of the incorporation of epoxidized jatropha oil (EJO) as a plasticizer and alkaline treatment of kenaf fiber on the thermal properties of PLA/Kenaf/EJO biocomposites. Kenaf fiber was treated with 6% sodium hydroxide (NaOH) solution for 4 h. The thermal properties of the biocomposites were analyzed using a differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). It must be highlighted that the addition of EJO resulted in a decrease of glass transition temperature which aided PLA chain mobility in the blend as predicted. TGA demonstrated that the presence of treated kenaf fiber together with EJO in the blends reduced the rate of decomposition of PLA and enhanced the thermal stability of the blend. The treatment showed a rougher surface fiber in scanning electron microscopy (SEM) micrographs and had a greater mechanical locking with matrix, and this was further supported with Fourier-transform infrared spectroscopy (FTIR) analysis. Overall, the increasing content of EJO as a plasticizer has improved the thermal properties of PLA/Kenaf/EJO biocomposites.
    Matched MeSH terms: Hibiscus
  7. Abdul Hamid Z, Lin Lin WH, Abdalla BJ, Bee Yuen O, Latif ES, Mohamed J, et al.
    ScientificWorldJournal, 2014;2014:258192.
    PMID: 25405216 DOI: 10.1155/2014/258192
    Hematopoietic stem cells- (HSCs-) based therapy requires ex vivo expansion of HSCs prior to therapeutic use. However, ex vivo culture was reported to promote excessive production of reactive oxygen species (ROS), exposing HSCs to oxidative damage. Efforts to overcome this limitation include the use of antioxidants. In this study, the role of Hibiscus sabdariffa L. (Roselle) in maintenance of cultured murine bone marrow-derived HSCs was investigated. Aqueous extract of Roselle was added at varying concentrations (0-1000 ng/mL) for 24 hours to the freshly isolated murine bone marrow cells (BMCs) cultures. Effects of Roselle on cell viability, reactive oxygen species (ROS) production, glutathione (GSH) level, superoxide dismutase (SOD) activity, and DNA damage were investigated. Roselle enhanced the survival (P < 0.05) of BMCs at 500 and 1000 ng/mL, increased survival of Sca-1(+) cells (HSCs) at 500 ng/mL, and maintained HSCs phenotype as shown from nonremarkable changes of surface marker antigen (Sca-1) expression in all experimental groups. Roselle increased (P < 0.05) the GSH level and SOD activity but the level of reactive oxygen species (ROS) was unaffected. Moreover, Roselle showed significant cellular genoprotective potency against H2O2-induced DNA damage. Conclusively, Roselle shows novel property as potential supplement and genoprotectant against oxidative damage to cultured HSCs.
    Matched MeSH terms: Hibiscus*
  8. Mohamed J, Shing SW, Idris MH, Budin SB, Zainalabidin S
    Clinics (Sao Paulo), 2013 Oct;68(10):1358-63.
    PMID: 24212844 DOI: 10.6061/clinics/2013(10)11
    OBJECTIVES: The aim of this study was to investigate the protective effects of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell (RBC) membrane oxidative stress in rats with streptozotocin-induced diabetes.

    METHODS: Forty male Sprague-Dawley rats weighing 230-250 g were randomly divided into four groups (n = 10 rats each): control group (N), roselle-treated control group, diabetic group, and roselle-treated diabetic group. Roselle was administered by force-feeding with aqueous extracts of roselle (100 mg/kg body weight) for 28 days.

    RESULTS: The results demonstrated that the malondialdehyde levels of the red blood cell membranes in the diabetic group were significantly higher than the levels in the roselle-treated control and roselle-treated diabetic groups. The protein carbonyl level was significantly higher in the roselle-treated diabetic group than in the roselle-treated control group but lower than that in the diabetic group. A significant increase in the red blood cell membrane superoxide dismutase enzyme was found in roselle-treated diabetic rats compared with roselle-treated control rats and diabetic rats. The total protein level of the red blood cell membrane, osmotic fragility, and red blood cell morphology were maintained.

    CONCLUSION: The present study demonstrates that aqueous extracts of roselle possess a protective effect against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. These data suggest that roselle can be used as a natural antioxidative supplement in the prevention of oxidative damage in diabetic patients.

    Matched MeSH terms: Hibiscus/chemistry*
  9. Razak NI, Ibrahim NA, Zainuddin N, Rayung M, Saad WZ
    Molecules, 2014;19(3):2957-68.
    PMID: 24609017 DOI: 10.3390/molecules19032957
    Bleaching treatment of kenaf fiber was performed in alkaline medium containing hydrogen peroxide solution maintained at pH 11 and 80 °C for 60 min. The bleached kenaf fiber was analyzed using Fourier Transform Infrared (FTIR) and X-ray Diffraction (XRD) analysis. The bleached kenaf fiber was then compounded with poly-(lactic acid) (PLA) via a melt blending method. The mechanical (tensile, flexural and impact) performance of the product was tested. The fiber treatment improved the mechanical properties of PLA/bleached kenaf fiber composites. Scanning electron micrograph (SEM) morphological analysis showed improvement of the interfacial adhesion between the fiber surface and polymer matrix.
    Matched MeSH terms: Hibiscus/chemistry*
  10. Zakaria Razak, Abu Bakar Sulong, Norhamidi Muhamad, Che Hassan Che Haron, Mohd Khairol Fadzly Md Radzi, Dulina Tholibon, et al.
    Sains Malaysiana, 2018;47:1285-1291.
    Composite materials have increasingly become crucial in manufacturing engineering products and producing commodity
    materials in the major industries including; automotive, aerospace, marine, construction, agriculture and health science.
    However, several improvements regarding the strength, dimensional stability and the cost of production are required.
    In this study, composite of Kenaf, multi-wall carbon nanotube (MWCNT) and polypropylene (PP) with maleic anhydridegrafted
    polypropylene (MAPP) are examined. The results highlight that increasing MAPP loading, in turn, increases the
    value of the mechanical properties. The composites are produced by blending kenaf/MWCNT/PP using a Sigma blade
    mixer and injection moulding. Injection moulding is a significant operation used to produce plastic products. In the
    study, Kenaf core fibre was mixed with MWCNT and polypropylene, in addition to MAPP. The MAPP is added by applying
    different percentage (1, 2, 3 and 4 wt. %) during the blending process. The main objective of the study was to analyse the
    effects of MAPP concentrations on the mechanical properties of the Kenaf/MWCNT/PP composite. The results of the study
    established that MAPP 3 wt. % concentration with MWCNT 3 wt. % loading and Kenaf 30 wt. % filler provide optimum
    results for the composites. There was approximately, a 21% enhance in tensile strength of Kenaf 30 wt. %/MWCNT, 3 wt.
    %/MAPP, 3 wt. %/PP observed compared to the (without) MAPP composite. The composites with coupling agent stimulate
    better filler dispersion between Kenaf, MWCNT and PP observed using a scanning electron microscope (SEM) and fieldemission
    scanning electron microscope (FESEM).
    Matched MeSH terms: Hibiscus
  11. Sharifah Hanisah S.A. Aziz, Khairul Zaman Mohd Dahlan, Jalaluddin Harun
    MyJurnal
    At present, research in composite materials is being directed at using natural fibres instead of synthetic fibres. Kenaf which is extensively grown in the Far East including Malaysia, has been identified as a bast (stem) fibre with significant market potential. In this work, long and random kenaf fibres (non-woven mat form) were used in the as-received condition and alkalized with a 0.06M NaOH solution. They were combined with polypropylene thin sheets, sandwiched between layers of kenaf fibres and hot-pressed to form natural fibre composites. The mechanical properties of the composites were investigated to observe the effect of fibre alignment, fibre treatment and pre-irradiation method used. A general trend was observed whereby alkalized and long fibre composites gave higher flexural modulus and flexural strength compared with random mat and untreated fibres. The long fibre composites also gave a higher work of fracture. However, the correlation between fibre surface treatment and the work of fracture was less clear. Pre-irradiation on the polypropylene pellets and fibres before the composite is manufactured showed significant improvement on the flexural modulus and flexural strength. The bond performance test performed on the treated composites demonstrated good bonding and interfacial adhesion between the fibre and matrix. However, the method of moulding used need to be improved to optimize the performance of the composites.
    Matched MeSH terms: Hibiscus
  12. Chew LY, Teng SK, Neo YP, Sim YY, Chew SC
    J Oleo Sci, 2024;73(3):275-292.
    PMID: 38432993 DOI: 10.5650/jos.ess23111
    Roselle is an annual botanical plant that widely planted in different countries worldwide. Its different parts, including seeds, leaves, and calyces, can offer multi-purpose applications with economic importance. The present review discusses the detailed profile of bioactive compounds present in roselle seeds, leaves, and calyces, as well as their extraction and processing, to explore their potential application in pharmaceutical, cosmetic, nutraceutical, food and other industries. Roselle seeds with high phenolics, fiber, and protein contents, which are suitable to use in functional food product development. Besides, roselle seeds can yield 17-20% of roselle seed oil with high content of linoleic acid (35.0-45.3%) and oleic acid (27.1- 36.9%). This unique fatty acid composition of roselle seed oil makes it suitable to use as edible oil to offer the health benefits of essential fatty acid. Moreover, high contents of tocopherols, phenolics, and phytosterols were detected in roselle seed oil to provide nutritional, pharmaceutical, and therapeutic properties. On the other hand, roselle leaves with valuable contents of phenols, flavonoids, organic acid, and tocopherols can be applied in silver nanoparticles, food product development, and the pharmaceutical industry. Roselle calyces with high content of anthocyanins, protocatechuic acids, and organic acids are widely applied in food and colorant industries.
    Matched MeSH terms: Hibiscus*
  13. Gan S, Zakaria S, Chia CH, Kaco H, Padzil FN
    Carbohydr Polym, 2014 Jun 15;106:160-5.
    PMID: 24721064 DOI: 10.1016/j.carbpol.2014.01.076
    Cellulose carbamate (CCs) was produced from kenaf core pulp (KCP) using microwave reactor-assisted method. The effects of urea concentration and reaction time on the formation of nitrogen content in CCs were investigated. The CCs' solubility in LiOH/urea system was determined and its membranes were characterized. As the urea content and reaction time increased, the nitrogen content form in CCs increased which enhanced the CCs' solubility. The formation of CCs was confirmed by Fourier transform infrared spectroscopy (FT-IR) and nitrogen content analysis. The CCs' morphology was examined using Scanning electron microscopy (SEM). The cellulose II and crystallinity index of the membranes were confirmed by X-ray diffraction (XRD). The pore size of the membrane displayed upward trend with respect to the urea content observed under Field emission scanning electron microscope (FESEM). This investigation provides a simple and efficient procedure of CCs determination which is useful in producing environmental friendly regenerated CCs.
    Matched MeSH terms: Hibiscus/chemistry*
  14. Gan S, Piao SH, Choi HJ, Zakaria S, Chia CH
    Carbohydr Polym, 2016 Feb 10;137:693-700.
    PMID: 26686181 DOI: 10.1016/j.carbpol.2015.11.035
    Cellulose carbamate (CC) was produced from kenaf core pulp (KCP) via a microwave reactor-assisted method. The formation of CC was confirmed by Fourier transform infrared spectroscopy and nitrogen content analysis. The degree of substitution, zeta potential and size distribution of CC were also determined. The CC was characterized with scanning electron microscopy, X-ray diffraction and thermogravimetry analysis. The CC particles were then dispersed in silicone oil to prepare CC-based anhydrous electric stimuli-responsive electrorheological (ER) fluids. Rhelogical measurement was carried out using rotational rheometer with a high voltage generator in both steady and oscillatory shear modes to examine the effect of electric field strength on the ER characteristics. The results showed that the increase in electric field strength has enhanced the ER properties of CC-based ER fluid due to the chain formation induced by electric polarization among the particles.
    Matched MeSH terms: Hibiscus/chemistry*
  15. Cheong AM, Tan CP, Nyam KL
    J Food Sci, 2018 Oct;83(10):2457-2465.
    PMID: 30178877 DOI: 10.1111/1750-3841.14332
    Kenaf seed oil-in-water nanoemulsions (NANO) stabilized by sodium caseinate (SC), beta-cyclodextrin (β-CD), and Tween 20 (T20) have been optimized and shown to improve in vitro bioaccessibility and physicochemical stability in the previous study. The main objective of this study was to evaluate the stability of bioactive compounds and antioxidants in the NANO during storage at different temperatures (4 °C, 25 °C, and 40 °C). An evaluation of the antioxidant activities of each emulsifier showed that SC had good scavenging capability with 97.6% ABTS radical scavenging activity. Therefore, SC which was used as one of the main emulsifiers could further enhanced the antioxidant activity of NANO. At week 8 of storage, NANO that stored at 4 °C had maintained the best bioactive compounds stability and antioxidant activities with 90% retention of vitamin E and 65% retention of phytosterols. These results suggested that 4 °C would be the most suitable storage temperature for NANO containing naturally present vitamin E and phytosterols. From the accelerated storage results at 40 °C, NANO containing vitamin E and phytosterols had maintained half of its initial concentration until week 4 and week 2 of storage, which is equivalent to 16 weeks and 8 weeks of storage at room temperature, respectively.

    PRACTICAL APPLICATION: The results of this study provide a better understanding on the stability of bioactive compounds and antioxidant activities in oil-in-water nanoemulsions that stabilized by similar ternary emulsifiers during storage at different temperatures. In addition, this study could be used as a predictive model to estimate the shelf life of bioactive compounds encapsulated in the form of nanoemulsions.

    Matched MeSH terms: Hibiscus/chemistry*
  16. Halimatul, S.M.N., Amin, I., Mohd.-Esa, N., Nawalyah, A.G., Siti Muskinah, M.
    MyJurnal
    This study investigated the protein quality of two sets of Roselle seeds processed differently (dried and boiled). Twenty weanling Sprague Dawley rats were used to conduct the growth and nitrogen balance studies. Rats were fed with 10% (w/w) protein from dried (DS) and boiled (BS) Roselle seeds powder for 4 weeks. Casein was used in this study as a standard reference protein. There was a significantly higher (p < 0.05) food intake and weight gain by rats fed with BS compared with DS. In the growth study, there was no significant difference (p < 0.05) in protein efficiency ratio (PER) and net protein ratio (NPR) of BS compared to DS, but it was significantly different with casein (CD). PER value of rats fed with DS was significantly lower (p < 0.05) than casein. In the nitrogen balance study, true nitrogen absorption (TNA) and nitrogen balance (NB) of BS group was significantly higher (p < 0.05) than DS group. However, apparent digestibility (AD), true digestibility (TD) and biological value (BV) for both diets was not significantly different. This study showed that the protein quality of dried Roselle seeds was similar to the Roselle seeds boiled at 100oC for 30 minutes.
    Matched MeSH terms: Hibiscus
  17. NUR FIKRIAH HASHIM, NURAQILAH MOHD ZAINAL, NURAIN JAMIL, NURUL NASUHA MOHD NOR, SURIANI MAT JUSOH
    MyJurnal
    Nowadays, Kenaf fiber is sustainably useful in marine structures and has become one of the materials that may be high in demand as it is light, biodegradable and environmental friendly. This study investigates the effect of fiber percentage on compressive strength of fiber reinforced concrete (FRC) and the relationship between compressive strength and time of FRC immersion in seawater. FRC concrete cubes were prepared using four different percentage of fiber (0%, 1.5%, 3.0% and 4.5%). These FRC were immersed in seawater for 7, 14 and 21 days for three consecutive weeks. Based on the experiment, it was found that there was improvement in compressive strength of FRC when compared to plain cement concrete. The results showedthat 3.0% of KF to cement matrix concrete determined the highest compressive strength of 205.43 Pa while 0% of KF fiber to cement concrete matrix (control specimen) showed the lowest compressive strength of 158.28 Pa. Also the addition of Kenaf fiber to cement concrete decreased the seawater absorption more than concrete with absolutely 0% of KF fiber to cement concrete (control specimen). In conclusion, the results did show significant improvement and a consistent trend on strength with the addition of FRC. This study also revealed that the percentage of water absorption was on the increase for 0, 7 and 14 days and become constant after day 21. This is due to manufacturing defects that occurred which block the water from entering the material and making the material absorb less water.
    Matched MeSH terms: Hibiscus
  18. Mohamad, O., Mohd. Nazir, B., Abdul Rahman, M., Herman, S.
    MyJurnal
    Roselle (Hibiscus sabdariffa L.) from Malvaceae family is an annual crop with potential for use by many industries including food, animal feed, neutraceutical, cosmeticeutical and pharmaceutical industries. Its origin is not fully known but it is believed to be from West Africa, although the plant is found native from India to Southeast Asia. The calyxes, stems and leaves are acid in flavour. The juice is produced from the calyces, and it is claimed to be a prohealth drink due to its high contents of ascorbic acid, anthocyanins and other antioxidants.
    Matched MeSH terms: Hibiscus
  19. Si LY, Kamisah Y, Ramalingam A, Lim YC, Budin SB, Zainalabidin S
    Appl Physiol Nutr Metab, 2017 Jul;42(7):765-772.
    PMID: 28249121 DOI: 10.1139/apnm-2016-0506
    Vascular endothelial dysfunction (VED) plays an important role in the initiation of cardiovascular diseases. Roselle, enriched with antioxidants, demonstrates high potential in alleviating hypertension. This study was undertaken to investigate the effects of roselle supplementation of VED and remodelling in a rodent model with prolonged nicotine administration. Male Sprague-Dawley rats (n = 6 per group) were administered with 0.6 mg/kg nicotine for 28 days to induce VED. The rats were given either aqueous roselle (100 mg/kg) or normal saline orally 30 min prior to nicotine injection daily. One additional group of rats served as control. Thoracic aorta was isolated from rats to measure vascular reactivity, vascular remodelling and oxidative stress. Roselle significantly lowered aortic sensitivity to phenylephrine-induced vasoconstriction (Endo-(+) Cmax = 234.5 ± 3.9%, Endo-(-) Cmax = 247.6 ± 5.2%) compared with untreated nicotine group (Endo-(+) Cmax = 264.5 ± 6.9%, Endo-(-) Cmax = 276.5 ± 6.8%). Roselle also improved aortic response to endothelium-dependent vasodilator, acetylcholine (Endo-(+) Rmax = 73.2 ± 2.1%, Endo-(-) Rmax = 26.2 ± 0.8%) compared to nicotine group (Endo-(+) Rmax = 57.8 ± 1.7%, Endo-(-) Rmax = 20.9 ± 0.8%). In addition, roselle prevented an increase in intimal media thickness and elastic lamellae proliferation to preserve vascular architecture. Moreover, we also observed a significantly lowered degree of oxidative stress in parallel with increased antioxidant enzymes in aortic tissues of the roselle-treated group. This study demonstrated that roselle prevents VED and remodelling, and as such it has high nutraceutical value as supplement to prevent cardiovascular diseases.
    Matched MeSH terms: Hibiscus/chemistry*
  20. Lim YC, Budin SB, Othman F, Latip J, Zainalabidin S
    Cardiovasc Toxicol, 2017 Jul;17(3):251-259.
    PMID: 27402292 DOI: 10.1007/s12012-016-9379-6
    Roselle (Hibiscus sabdariffa Linn.) calyces have demonstrated propitious cardioprotective effects in animal and clinical studies; however, little is known about its action on cardiac mechanical function. This study was undertaken to investigate direct action of roselle polyphenols (RP) on cardiac function in Langendorff-perfused rat hearts. We utilized RP extract which consists of 12 flavonoids and seven phenolic acids (as shown by HPLC profiling) and has a safe concentration range between 125 and 500 μg/ml in this study. Direct perfusion of RP in concentration-dependent manner lowered systolic function of the heart as shown by lowered LVDP and dP/dtmax, suggesting a negative inotropic effect. RP also reduced heart rate (negative chronotropic action) while simultaneously increasing maximal velocity of relaxation (positive lusitropic action). Conversely, RP perfusion increased coronary pressure, an indicator for improvement in coronary blood flow. Inotropic responses elicited by pharmacological agonists for L-type Ca2+channel [(±)-Bay K 8644], ryanodine receptor (4-chloro-m-cresol), β-adrenergic receptor (isoproterenol) and SERCA blocker (thapsigargin) were all abolished by RP. In conclusion, RP elicits negative inotropic, negative chronotropic and positive lusitropic responses by possibly modulating calcium entry, release and reuptake in the heart. Our findings have shown the potential use of RP as a therapeutic agent to treat conditions like arrhythmia.
    Matched MeSH terms: Hibiscus*
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