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Optimization of illumina truseq targeted RNA Expression (TREx) library quality

Nadiah Abu, Noraini Nordin, Noorjahan Banu Alitheen, Nadiah Abu, Sheau Wei Tan, Swee Keong Yeap, et al.

Sains Malaysiana, 2018;47:303-308.

RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyA-enriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA.
Effect of extraction solvents and drying conditions on total phenolic content and antioxidant properties of watermelon rind powder

Lee-Hoon Ho, Nor Fadhilah Ramli, Norlia Muhamad, Mohd Nizam Haron, Thuan-Chew Tan

Sains Malaysiana, 2018;47:99-107.

The objective of the present study was to determine the total phenolic content (TPC), total flavonoid content (TFC) and
antioxidant properties, i.e. 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and ferric reducing
antioxidant potential (FRAP) assay, of red- and yellow-fleshed watermelon rind powders prepared using different drying
conditions (hot-air oven drying at 40 and 60°C and freeze drying). All the samples were subjected to four different
solvent extract using water, methanol, ethanol and acetone prior analyses. Water extract from red- and yellow-fleshed
watermelon rind powders presented highest value for TPC and TFC. However, methanol extract samples showed highest
value for antioxidant properties (DPPH and FRAP) followed by acetone, ethanol and water extract. By comparing the
drying conditions, all samples dried using hot-air dryer at 40 and 60°C had significantly higher (p<0.05) in TPC value
than the samples dried using freeze dryer. However, samples dried using freeze dryer showed highest in DPPH and
FRAP values. The present obtained results would be useful to the food and pharmaceutical industries for developing of
functional ingredients.
Fulltext Lard classification from other animal fats using Dielectric Spectroscopy technique

Amat Sairin, M., Abd Aziz, S., Tan, C.P., Mustafa, S., Abd Gani, S.S., Rokhani, F.Z.

International Food Research Journal, 2019;26(3):773-782.
MyJurnal

Lard adulteration in processed foods is a major public concern as it involves religion and
health. Most lard discriminating works require huge lab-based equipment and complex sample
preparation. The objective of the present work was to assess the feasibility of dielectric
spectroscopy as a method for classification of fats from different animal sources, in particular,
lard. The dielectric spectra of each animal fat were measured in the radio frequency of 100
Hz – 100 kHz at 45°C to 55°C. The fatty acid composition of each fat was studied by using
data from gas chromatography mass spectrometry (GCMS) to explain the dielectric behaviour
of each fat. The principal component analysis (PCA) and artificial neural network (ANN)
were used to classify different animal fats based on their dielectric spectra. It was found that
lard showed the highest dielectric constant spectra among other animal fats, and was mainly
affected by the composition of C16 and C18 fatty acids. PCA classification plot showed clear
performance in classifying different animal fats. Finally, ANN classification showed different
animal fats were classified into their respective groups effectively at high accuracy of 85%.
Dielectric spectroscopy, in combination with quantitative analysis, was concluded to provide
rapid method to discriminate lard from other animal fats.
Genetic polymorphisms of interleukin-4 third intron region in the Malaysian patients with systemic lupus erythematosus

Chua KH, Kee BP, Tan SY, Lian LH

Journal of Medical Sciences, 2008;8:437-442.
DOI: 10.3923/jms.2008.437.442

In this study, we analysed the genetic polymorphisms present in the third intron region of Interleukin-4 gene in Malaysian patients with Systemic Lupus Erythematosus. Overall, the RP I and II alleles were found evenly distributed in both the SLE patients and control individuals. There was no significant association observed in the distribution of allelic and genotypic frequencies between SLE patients and healthy controls. The result obtained is similar to a previous study carried out on SLE Chinese patients in Taiwan.
Role of endothelial dysfunction in cardiovascular disease: a review

Amudha K, Tan KH, Choy AM, Mohd Rais M, Lang CC

JUMMEC, 2000;5:59-66.
Depression, hormonal status and erectile dysfunction in the aging male: results from a community study in Malaysia

Low WY, Khoo EM, Tan HM, Hew FL, Teoh SH

Journal of Men's Health and Gender, 2006;3:263-270.
DOI: 10.1016/j.jmhg.2006.02.007

Background: Depression and erectile dysfunction (ED) are common in aging and the two conditions often co-exist. These conditions have been shown to be associated with hormonal changes in men. This paper examines the association between depression, ED, and hormonal status of men aged above 50 years in the Klang Valley, Malaysia.
Methods: Five hundred men aged 50 years and above were randomly selected via the electoral roll and invited to participate in a community-based study on men's health: 351 men responded. Respondents were interviewed individually based on a self-developed questionnaire, which included information on socio-demographic data. Erectile function was measured using the International Index for Erectile Function-5 (IIEF-5) and depression was measured using the 15 item Geriatric Depression Scale (GDS-15).
Results: Sixty-nine percent of the men were diagnosed with ED. Mean GDS score was 3.33 (SD = 3.29). Nineteen percent (n = 67) of the men had abnormal levels of testosterone (≤11 nmol/l) and this comprised 73% of men with ED (n = 49) and 27% of men without ED (n = 18). There was no significant association between testosterone level and ED (χ2 = 0.68, p = 0.41). Significant association was found between depression (GDS ≥ 5) and men with ED (χ2 = 6.07, p = 0.014). Sex hormone binding globulin and luteinising hormone were negatively correlated with erectile function. Results of the multiple linear regression showed that age and depression are predictors of erectile function.
Conclusion: Depression and ED should be screened for when either exists in the male patient and treatment directed accordingly. © 2006 WPMH GmbH.
Erectile dysfunction (ED), lower urinary tract symptoms (LUTS) and testosterone deficiency (TD): Men's major concern (MMC) - An important portal for promoting men's health?

Tan WP, Fong ZV, Tong SF, Low WY, Tan HM

Journal of Men's Health, 2011;8:S81-S83.
DOI: 10.1016/S1875-6867(11)60029-8

Background: Men's major concern (MMC) is a common quality of life issue affecting the aging male population. This paper examines the association between MMC and cardiometabolic diseases in Malaysian men in an urban setting.
Methods: A cross-sectional community based study was conducted in an urban area involving 1046 men, aged over 40 years and above, who were randomly selected using the electoral roll in Selangor Malaysia. Participants were assessed by trained doctors using structured questionnaire which included medical history, IIEF-5 to assess men's erectile dysfunction and IPSS score to assess prostatic symptoms. Clinical assessments including blood pressure, weight, height and waist circumference were performed. In addition, biochemical assessments for fasting blood glucose and lipid profile were done.
Results: The response rate was 62.8%. The mean age of the participants was 55.8±8.4 (range: 41-93) years. Around two fifth of the participants (39.8%) had no MMC, 28.4% were diagnosed with 1 MMC, 9.9% with 2MMCs and 1.2% with 3MMCs. Increasing number of MMCs were significantly associated with increasing rate of cardiometabolic diseases (No MMC = 26.2%, 1 MMC = 43.4%, 2 MMCs = 63.5% and 3 MMCs = 69.2%) (P < 0.01) and metabolic syndrome based on Asian criteria (No MMC = 21.5%, 1 MMC = 39.0%, 2 MMCs = 49.1%, 3 MMCs = 62.5%) (P < 0.01). Participants with one or more MMC significantly showed a higher proportion of undiagnosed diabetes as well as reported diabetes (No MMC = 1.7%, 1 or more MMCs = 3.6%) when compared to participants without MMC (P ≤ 0.01).
Conclusions: Men's Major Concerns (e.g., ED, LUTS and TD) were associated with cardiometabolic diseases and collectively, they could be used as an important portal to men's health. © 2011 WPMH GmbH.
Fulltext Psychiatrists in malaysia: the ratio and distribution

Lee, Toh Chin, Ng, Chong Guan, Benedict Francis, Tan, Shean Yen

Malaysian Journal of Psychiatry, 2018;27(1):0-0.
MyJurnal

We are aware of the shortage of psychiatrists in Malaysia.
However, there is no formal report on the distribution and ratio of
psychiatrists in each state in Malaysia. We aim to do a detailed count of the
number of psychiatrists in the country. Methods: We obtained the figures for
the psychiatrists practicing in the government, private and university settings
by accessing the Ministry of Health database, information from the
Malaysian Psychiatric Association, Malaysian Mental Health Association,
National Specialist Register and websites of the respective Universities. The
total number and ratio of psychiatrists per 100,000 population are calculated.
Results: There was a total of 410 registered psychiatrists in Malaysia in the
private universities, private clinics, public universities and government
hospitals. The state with the highest number of psychiatrists is Wilayah
Persekutuan Kuala Lumpur with a total of 94 psychiatrists which has a ratio
of 5.24 per 100, 000 population followed by Wilayah Persekutuan Putrajaya
with 3.38 per 100,000 population. The states with the least ratio of
psychiatrists are Sabah with 0.54 and Kedah with 0.55 per 100, 000
population. Conclusion: There is a discrepancy in the geographical
distribution of psychiatrists in Malaysia. People living in larger, urban states
have better access to mental health care whereas the smaller states face a
serious lack of psychiatrists. More effort should be taken to improve mental
healthcare in Malaysia as recommended by WHO with one psychiatrist for
every 10, 000 population.
Problem-based learning (PBL): making an impact in education

Azila NMA, Sim SM, Tan CPL, Alhady SF

JUMMEC, 1999;4<I> </I>:94-98.

Problem-based learning (PBL) i s an educational reform that is now becoming a household word in higher education, particularly in medical schools. Many medical schools have implemented a full problem-based learning curriculum (PBLC) whiIe some have included PBL into selected units of the course in an otherwise conventional cumculum (embedded PBL) and others run their tutorials in a PBL manner within a modified conventional curriculum (hybrid curriculum). Yet there are others who claim that small components of PBL in a conventional curriculum are not PBL at all. Thus amateurs in the subject matter find difficulty in evaluating the logistics and outcome of these variations. This article focuses or, the general characteristics of PBL and how this learning method can help enhance independent learning and critical thinking, whether in a full, embedded or hybrid curriculum. The extent of PBL to be included and which of the three types is to be adopted depends on the objective of the undergraduate medical course as determined by the faculty, resources available, limitations, feedback on the existing curriculum and various other factors. KEYWORDS: Problem-based Learning (PBL); Embedded PBL; Hybrid PBL; New Integrated Curriculum (NIC).
Fulltext Effectiveness of vision screening training module in improving the level of knowledge on vision screening among pre-school teachers in Klang Valley Malaysia

Omar, R., Knight, V.F., Tan, X.L., Ahmad Zahidi A.A., Mohd Saat, N.Z.

JUMMEC, 2019;22(1):58-65.
MyJurnal

Empowering lay screeners, such as pre-school teachers, on vision screening is a cost-effective way to
ensure larger populations of children can be screened. Although the validity of lay screeners in conducting
vision screening were reported in several studies, none showed data concerning improvement of the level
of knowledge among lay screeners after completing vision screening training, which could indicate the
effectiveness of the training program. This study aimed to determine the level of knowledge of pre-school
teachers before and after attending a training program. Sixty pre-school teachers from Tabika and Taska KEMAS
were randomly selected. The Study Group (n = 30) was given theory and practical training on vision screening,
whereas the Control Group (n = 30) was only given brief verbal instructions on how to conduct the screening. A
theory test containing 15 questions related to the training modules were administered to both groups, before
and after their training/briefing respectively. The findings showed that the level of knowledge among preschool
teachers in the Study Group (73.24 ± 11.73%) was significantly higher than the Control Group (56.22
± 13.11%) (p < 0.01). There was also a significant improvement in the level of knowledge among pre-school
teachers in the Study Group after the training (p
Older age and duration of exposure to type 2 diabetes in selective screening of asymptomatic carotid artery stenosis for primary stroke prevention-A single institution experience

Safri LS, Lip HTC, Saripan MI, Huei TJ, Krishna K, Md Idris MA, et al.

Prim Care Diabetes, 2020 08;14(4):364-369.
PMID: 31744790 DOI: 10.1016/j.pcd.2019.10.001

AIMS: To evaluate the incidence and risk factors for carotid artery stenosis amongst asymptomatic type 2 diabetes from a single Malaysian tertiary institution.
METHODS: This is a prospective cross-sectional study of asymptomatic type 2 diabetics selected from the outpatient ophthalmology and endocrine clinics for carotid duplex ultrasound scanning performed by a single radiologist. The duplex ultrasound criteria were based on the North American Symptomatic Carotid Endarterectomy Trial (NASCET) classification of carotid artery stenosis. Univariate and multivariate analysis was performed to identify possible risk factors of carotid artery stenosis.
RESULTS: Amongst the 200 patients, the majority were males (56%) and Malay predominance (58.5%). There were 12/200 patients (6%) with mean age of 69.2 years identified to have carotid artery stenosis. Univariate analysis of patients with asymptomatic carotid artery stenosis identified older age of 69.2 years (p=0.027) and duration of exposure to diabetes of 17.9 years (p=0.024) as significant risk factors.
CONCLUSION: Patients with longer exposure of diabetes and older age were risk factors of carotid artery stenosis in asymptomatic type 2 diabetics. These patients should be considered for selective screening of carotid artery stenosis during primary care visit for early identification and closer surveillance for stroke prevention.
Fulltext Predictors of mortality and validation of burn mortality prognostic scores in a Malaysian burns intensive care unit

Lip HTC, Idris MAM, Imran FH, Azmah TN, Huei TJ, Thomas M

BMC Emerg Med, 2019 11 07;19(1):66.
PMID: 31699024 DOI: 10.1186/s12873-019-0284-8

BACKGROUND: Majority burn mortality prognostic scores were developed and validated in western populations. The primary objective of this study was to evaluate and identify possible risk factors which may be used to predict burns mortality in a local Malaysian burns intensive care unit. The secondary objective was to validate the five well known burn prognostic scores (Baux score, Abbreviated Burn Severity Index (ABSI) score, Ryan score, Belgium Outcome Burn Injury (BOBI) score and revised Baux score) to predict burn mortality prediction.
METHODS: Patients that were treated at the Hospital Sultan Ismail's Burns Intensive Care (BICU) unit for acute burn injuries between 1 January 2010 to 31 December 2017 were included. Risk factors to predict in-patient burn mortality were gender, age, mechanism of injury, total body surface area burn (TBSA), inhalational injury, mechanical ventilation, presence of tracheotomy, time from of burn injury to BICU admission and initial centre of first emergency treatment was administered. These variables were analysed using univariate and multivariate analysis for the outcomes of death. All patients were scored retrospectively using the five-burn mortality prognostic scores. Predictive ability for burn mortality was analysed using the area under receiver operating curve (AUROC).
RESULTS: A total of 525 patients (372 males and 153 females) with mean age of 34.5 ± 14.6 years were included. There were 463 survivors and 62 deaths (11.8% mortality rate). The outcome of the primary objective showed that amongst the burn mortality risk factors that remained after multivariate analysis were older age (p = 0.004), wider TBSA burn (p
Fulltext Hepatitis B virus (HBV) and Hepatitis C virus (HCV) screening among pregnant women: a systematic review

Nur Farhana Mohamad, Izzuna Mudla Mohamed Ghazali, Junainah Sabirin, Tan Soek Siam, Rohani Jahis

Malaysian Journal of Medicine and Health Sciences, 2019;15(104):58-58.
MyJurnal

Introduction: Universal HBV and HCV screening among pregnant women is not a current practice in Malaysia. It is aimed to conduct a systematic review on available evidences in an effort to strengthen the national hepatitis screen-ing programs. Methods: Systematic search was performed from databases; Medline, Cochrane, PubMed and IN-AHTA. Relevant studies according to inclusion/exclusion criteria were critically appraised and evidence graded. Re-sults: From 782 titles identified, two systematic reviews, two retrospective cohort studies, two cross-sectional studies, one cost-utility analysis and one cost-effectiveness analysis were included. Universal antenatal HBV screening was associated with almost complete vaccination coverage for newborns. Replacing targeted screening with universal HBV screening was associated with increased identification of newborns indicated for HBV-immunization from 50% to 96%. Universal antenatal HBV screening had incremental cost-effectiveness ratio (ICER)s ranged from €2,032 to €26,181 per life year (LY) gained. As for HCV, targeted antenatal screening was associated with low HCV prevalence. Universal screening did not detect significantly more women with HCV infection than did targeted screening. One cost-effectiveness analysis found that universal antenatal HCV screening had ICER of €52,473 per LY gained and one cost-utility analysis reported ICER of £9,139 per QALY gained. Conclusion: Based on the above review, universal HBV screening in pregnant women is effective in increasing vaccination coverage for newborns. However, the ICERs had wide range. Therefore, local economic evaluation is needed to estimate cost implications before considering addition into national screening program. While for HCV, both universal and targeted screening in pregnant women had low detection rate thus high-risk approach screening is appropriate in Malaysia.
Could bacteriophages isolated from the sewage be the solution to methicillin-resistant Staphylococcus aureus?

Tan CS, Aqiludeen NA, Tan R, Gowbei A, Mijen AB, Santhana Raj L, et al.

Med J Malaysia, 2020 03;75(2):110-116.
PMID: 32281590

INTRODUCTIONS: The emergence of multidrug-resistant bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA) complicates the treatment of the simplest infection. Although glycopeptides such as vancomycin still proves to be effective in treating MRSA infections, the emergence of vancomycin-resistant strains limits the long term use of this antibiotic. Bacteriophages are ubiquitous bacterial viruses which is capable of infecting and killing bacteria including its antibiotic-resistant strains. Bactericidal bacteriophages use mechanisms that is distinct from antibiotics and is not affected by the antibioticresistant phenotypes.
OBJECTIVES: The study was undertaken to evaluate the possibility to isolate bacteriolytic bacteriophages against S.aureus from raw sewage water and examine their efficacy as antimicrobial agents in vitro.
METHODS: Bacteriophages were isolated from the raw sewage using the agar overlay method. Isolated bacteriophages were plaque purified to obtain homogenous bacteriophage isolates. The host range of the bacteriophages was determined using the spot test assay against the 25 MRSA and 36 MSSA isolates obtained from the Sarawak General Hospital. Staphylococcus saprophyticus, Staphylococcus sciuri and Staphylococcus xylosus were included as non-SA controls. The identity of the bacteriophages was identified via Transmission Electron Microscopy and genomic size analysis. Their stability at different pH and temperature were elucidated.
RESULTS: A total of 10 lytic bacteriophages infecting S.aureus were isolated and two of them namely ΦNUSA-1 and ΦNUSA-10 from the family of Myoviridae and Siphoviridae respectively exhibited exceptionally broad host range against >80% of MRSA and MSSA tested. Both bacteriophages were specific to S.aureus and stable at both physiologic pH and temperature.
CONCLUSION: This study demonstrated the abundance of S.aureus specific bacteriophages in raw sewage. Their high virulence against both MSSA and MRSA is an excellent antimicrobial characteristic which can be exploited for bacteriophage therapy against MRSA.
Fulltext Isolation of lignocellulose bioconversion bacteria for reducing sugar production from coconut husk

Mariam Aisha Fatima, Foo, Y.H, Tan, J.W, Nurain Nasrudin, Sangaran, P

Journal of Management & Science, 2019;17(2):23-31.
MyJurnal

Lignocellulose is the most abundant renewable resource naturally available with great potential for bioconversion to value-added bio-products in this case from coconut husk to reducing sugar. This study was to isolate the lignocellulose bioconversion bacteria from cow dung and termite gut for reducing sugar production. CMC and Congo red dye was used as a selective media to screen for cellulose degrading bacteria while alkaline lignin media used to screen for lignin degrading bacteria. Coconut husk was pretreated using alkaline precipitation method. The 16s rRNA sequence analysis, Gram staining, motility test, crystal formation test and penicillin sensitivity test were used to confirm the bacteria identity. The reducing sugars production was monitor using 3, 5-dinitrosalicyclic acid (DNS) method. Bacterial enzymatic hydrolysis were facilitated through fermentation of 5 locally isolated bacteria at 6 shaking regimes which were 0rpm, 50rpm, 100rpm, 150rpm, 200rpm and 250rpm and conditions were fixed at pH7.0 and temperature 25oC. The total of 39 and 28 colonies were isolated from cow dung and termite gut respectively. The isolates T10, T19, T22, T24, C19, and C37 were identified as Bacillus thuringiensis, Enterobacter aerogenes, Bacillus pumilus, Bacillus vireti, Bacillus anthracis, and Bacillus thuringiensis. In PC medium, 2 bacteria strains, T19 and C37 showed the highest concentration of reducing sugar production (after 18 to 20 hours) which was 1.28 g/L at 150rpm (T19) and 1.27 g/L at 200rpm (C37). While in CMC medium, T19 and C37 also showed the highest concentration of reducing sugar production which was 0.72 g/L at 150rpm (T19) and 0.70 g/L at 200rpm (C37). These bacteria demonstrated significant (p < 0.05) to degrade cellulose was shown by measuring the diameter of clearing zone signifying metabolism of cellulose and also the ability to convert both PC and CMC to reducing sugars measurable by DNS method. This study suggests that the PC resulted in higher reducing sugar production compare to CMC at 150rpm and 200rpm by E. aerogenes and B. thuringiensis which these bacteria isolated from cow dung and termite gut. Future studies on metabolic pathway and specific reducing sugar produced may be done using High Performance Liquid Chromatography (HPLC) analysis.
Fulltext Genome editing: a comparative study on the efficiency of CRISPR/Cas9 nuclease versus nickase using HIV as a model system

Ravichantar Nithya, Ishola Oluwaseun Ayodeji, Tan Benjy Jek Yang, Ong Jing Kai, Theva Das Kumitaa

Malaysian Journal of Medicine and Health Sciences, 2019;15(109):122-129.
MyJurnal

Introduction: CRISPR/Cas9 nuclease has gained popularity as a genome editing tool due to its straight-forward mechanism. However, there are concerns that CRISPR nuclease would cause off-target and toxicity. The CRISPR/ Cas9 D10A nickase was designed to enhance genome editing. Nevertheless, this raised the question of whether the efficiency of nickase is compromised compared to CRISPR/Cas9 nuclease. Targeting HIV genes, we investigated if CRISPR nuclease performed better than the nickase in efficacy and safety. Methods: CRISPR nucleases and nickases were designed to target Gag, Pol, Rev, Vif, Tat and LTR. HIV latently infected cell line, ACH-2, was transfected with the nucleases and nickases. Changes to viral load after CRISPR treatment was measured using p24 ELISA. Safety of nuclease and nickase was monitored using GFP expression with fluorescence microscopy and flow cytometry. Targeting two sites within the same gene, and targeting multiple genes concurrently were also studied to determine efficacy of CRISPR in reducing viral load. Results: A 44.9 to 68.1% and a 34.4 to 49.7% decrease in viral load was seen in CRISPR nuclease and nickase respectively. Microscopy and flow cytometry results showed that the nickase system was slightly toxic with a 0.31 to 0.7-fold cell death. There was a 34% decrease in viral load when two sites were targeted within a gene, and the largest decrease was seen when all the nucleases were combined, giving a 75.4% decrease in viral load at day 5. Conclusion: The knowledge gained from this study will be employed to im- prove genome editing in other disease models.
Fulltext Towards the data security and digital evidence based solution in Bangladesh perspective

Ahmed, Shekh Abdullah-Al-Musa, Nik Zulkarnaen Khidzir, Tan,Tse Guan

ZULFAQAR International Journal of Defence Science, Engineering & Technology, 2019;2(1):1-8.
MyJurnal

Data is anything in a form suitable for use with a computer. Data is often distinguished from programs. A program is a set of instructions that detail a task for the computer to perform. In this sense, data is thus everything that is not program code. Generally and in science, data is a gathered body of facts. Data security refers to protective digital privacy measures that are applied to prevent unauthorized access to computers data. Data security also protects data from corruption. However in this article would show a method for encryption text data by java program. It will encrypt is such a way that data could not read in plain text. Whereas Forensic science is a combine science. Scientific Evidence is a part of forensic science. By using the forensic science collecting information and present it on the court. So the meaning of evidence of science representing the understanding of collection information by establishes science. For example to create a DNA profile, following the establish protocol to make a DNA profile. So by testing it thousand times it will give the same result. This is the establish rule of science. When applying this rule in the court then it will call scientific Evidence. In Bangladesh most of the time it is seen that judiciary process is depends on confession based. Justice in the lower court does not depend on Digital forensic rather depend on Confession based. The definition of document is given in Section 3 at Evidence Act, 1872 and it is amended by ICT Act 2006 by Section 87 ,it is said that creating document by electronic is also a document .So any picture or video or audio are electronic document is a document .However for digital evidence based solution in this project using autopsy forensic tools, which will run on Kali Linux Forensic mode. It will generate a report paper and calculates MD5 hash values and confirms the integrity of the data before closing the files. Not all computer offence we can called cybercrime , but if a person created forged certificate or steal computer file , may called it as a Digital Crime .In real space , there are some physical force such as robbery , theft etc. But in Digital crime, there is no physical force, but doing the crime by technology.
Fulltext The role of negative automatic thoughts as a mediator in the relationship between maternal attachment and depressive symptoms among late adolescents of Pakistan

Siddrah Irfan, Nor Sheereen Zulkefly, Tan Kit Aun, Siti Irma Fadhillah Ismail

Malaysian Journal of Medicine and Health Sciences, 2019;15(101):21-29.
MyJurnal

Introduction: Negative automatic thoughts refer to subconscious thinking patterns in response to irrational and self-defeating stimuli. Individuals who are depressed tend to have biased negative automatic thoughts. Therefore, there is a need to refine our understanding of what contributes to the development of adolescent depressive symp- toms. The current study examined the mediating role of negative automatic thoughts in the link between maternal attachment and depressive symptoms among late adolescents in Pakistan. Methods: This cross-sectional study fo- cused on 936 (males=423, females=513) students aged 17 to 19 years old, who were selected through multistage cluster sampling technique. Respondents answered self-report questionnaires which assessed maternal attachment, depressive symptoms, and negative automatic thoughts. Structural equation modelling was utilised to analyse the relationships among the research variables. Results: The findings indicated that 39.3% had negative automatic thoughts; whereas, 5.6% experienced severe depressive symptoms. Correlational analysis showed that maternal attachment had a negative association with depressive symptoms and negative automatic thoughts. In contrast, de- pressive symptoms were positively related to negative automatic thoughts. Structural analysis revealed that negative automatic thoughts partially mediated the association of maternal attachment with depressive symptoms among late adolescents. Findings specifically demonstrated that those with insecure maternal attachment tend to have negative automatic thoughts, which subsequently lead to depressive symptoms. Conclusion: In conclusion, findings highlight the importance of secure attachment to the mother and positive automatic thoughts in combating depressive symp- toms; thus ensuring a healthy psychological development for adolescents.
Fulltext The effectiveness of educational programs on parenting stress and coping mechanism among parents of children with Autism Spectrum Disorder: a systematic review

Haytham Mohammad Al-Oran, Khuan Lee, Kit-Aun Tan, Poh Ying Lim

Malaysian Journal of Medicine and Health Sciences, 2019;15(3):135-141.
MyJurnal

The aim of this systematic review was to evaluate the effectiveness of educational programs on parenting stress and coping mechanism among parents of children with Autism Spectrum Disorder. Our current review retrieved the articles from databases such as CINAHL, Springer, Ovid, PubMed, Google Scholar, and EBSCO host. Only articles published between the years of 2000 and 2018 in these databases were recruited using keywords such as Autism Spectrum Disorder, education program, parenting stress, coping mechanism, and coping strategies. The search gen- erated 17 articles; 8 articles were relevant. This systematic review provides an important opportunity to advance our understanding of the effectiveness of the educational program for reducing parenting stress and improving coping mechanism among parents of children with Autism Spectrum Disorder. Nurses could also have a pivotal role in delivering the educational program for parents of children with ASD.
Fulltext A black finger does not equal a dead finger

Shalimar, A., William, H., Levin, K.B., Parminder G.S., Nur Azuatul, A.K., Tan, J.A.

Medicine & Health, 2018;13(2):229-234.
MyJurnal

Revascularisation and replant surgery occasionally produces sub-optimal results. We present two cases of near total amputation of the digits. The first patient was an 11-year-old boy with a middle finger avulsion injury sustained after the finger was caught in a washing machine with the flexor digitorum profundus still attached. The second patient was a 42-year-old male who sustained a machete attack and had near total amputation of his small and ring fingers with a 2 cm skin bridge still attached. All patients underwent revascularization with repair of only one digital artery for each finger as the opposite artery was non-viable. No digital veins were repaired. Relief of venous congestion was done by pin-prick six times a day up till one week. At one week post-surgery, the fingers were discoloured black but had good pulp turgor and full contour with arterial bleed on pin-prick. Inexperienced surgeons may view this as gangrene and amputate the fingers. However, we continued observation and at one month post-surgery, the black skin had desloughed and the underlying skin was pink.

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