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  1. Khare SK, Acharya UR
    Comput Biol Med, 2023 Mar;155:106676.
    PMID: 36827785 DOI: 10.1016/j.compbiomed.2023.106676
    BACKGROUND: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects a person's sleep, mood, anxiety, and learning. Early diagnosis and timely medication can help individuals with ADHD perform daily tasks without difficulty. Electroencephalogram (EEG) signals can help neurologists to detect ADHD by examining the changes occurring in it. The EEG signals are complex, non-linear, and non-stationary. It is difficult to find the subtle differences between ADHD and healthy control EEG signals visually. Also, making decisions from existing machine learning (ML) models do not guarantee similar performance (unreliable).

    METHOD: The paper explores a combination of variational mode decomposition (VMD), and Hilbert transform (HT) called VMD-HT to extract hidden information from EEG signals. Forty-one statistical parameters extracted from the absolute value of analytical mode functions (AMF) have been classified using the explainable boosted machine (EBM) model. The interpretability of the model is tested using statistical analysis and performance measurement. The importance of the features, channels and brain regions has been identified using the glass-box and black-box approach. The model's local and global explainability has been visualized using Local Interpretable Model-agnostic Explanations (LIME), SHapley Additive exPlanations (SHAP), Partial Dependence Plot (PDP), and Morris sensitivity. To the best of our knowledge, this is the first work that explores the explainability of the model prediction in ADHD detection, particularly for children.

    RESULTS: Our results show that the explainable model has provided an accuracy of 99.81%, a sensitivity of 99.78%, 99.84% specificity, an F-1 measure of 99.83%, the precision of 99.87%, a false detection rate of 0.13%, and Mathew's correlation coefficient, negative predicted value, and critical success index of 99.61%, 99.73%, and 99.66%, respectively in detecting the ADHD automatically with ten-fold cross-validation. The model has provided an area under the curve of 100% while the detection rate of 99.87% and 99.73% has been obtained for ADHD and HC, respectively.

    CONCLUSIONS: The model show that the interpretability and explainability of frontal region is highest compared to pre-frontal, central, parietal, occipital, and temporal regions. Our findings has provided important insight into the developed model which is highly reliable, robust, interpretable, and explainable for the clinicians to detect ADHD in children. Early and rapid ADHD diagnosis using robust explainable technologies may reduce the cost of treatment and lessen the number of patients undergoing lengthy diagnosis procedures.

    Matched MeSH terms: Electroencephalography/methods
  2. Kannan SK, Esakkiappa S, Anthonysamy E, Sudalaimuthu S, Sulaiman Y, Khan MM, et al.
    Mikrochim Acta, 2023 Feb 10;190(3):87.
    PMID: 36759372 DOI: 10.1007/s00604-023-05664-8
    Spermine (SPM) is considered a biomarker for prostate cancer and detecting it becomes highly challenging due to its electro- and optical-inactive nature. SPM has a tendency to interact with groups such as phosphates and sulfides to form macrocyclic arrangements known as nuclear aggregates of polyamines. Using this tendency, an electrochemical sensor has been developed using a polysulfide (PS) modified Au electrode (PS@Au electrode). PS has been synthesized from elemental sulfur by hydrothermal method and characterized using UV-Vis, fluorescence, FTIR, SEM, and XPS analyses. The PS@Au electrode was employed for electrochemical sensing of SPM. In the presence of SPM, a decrease in gold oxide reduction current was noted which is proportional to the concentration of SPM. The decrease in gold oxide reduction (0.5 V) current was attributed to the complexing nature of SPM-PS at the electrode interface. The reason for the decrease in current has been substantiated using XRF, XPS, and spectroelectrochemical studies. Under the optimized conditions, the PS@Au electrode exhibited a linear range of 1.55-250 µM with LOD of 0.511 ± 0.02 µM (3σ). The electrochemical strategy for SPM sensing exhibited better selectivity even in the presence of possible interferents. The selectivity stems from the selective interaction of SPM with PS on the Au electrode surface; the tested amino acids, and other molecules do not complex with PS and hence they could not interfere. The PS@Au electrode has been subjected to the determination of SPM in artificial urine samples and exhibited outstanding performance in the synthetic sample.
    Matched MeSH terms: Electrochemical Techniques/methods
  3. Aziz MHN, Safaruddin ADA, Hamzah NA, Supadi SS, Yuhao Z, Aziz MA
    Acta Biotheor, 2022 Nov 17;71(1):2.
    PMID: 36394646 DOI: 10.1007/s10441-022-09453-3
    A modified version of the SEIR model with the effects of vaccination and inter-state movement is proposed to simulate the spread of COVID-19 in Malaysia. A mathematical analysis of the proposed model was performed to derive the basic reproduction number. To enhance the model's forecasting capabilities, the model parameters were estimated using the Nelder-Mead simplex method by fitting the model outputs to the observed data. Our results showed a good fit between the model outputs and available data, where the model was then able to perform short-term predictions. In line with the rapid vaccination program, our model predicted that the COVID-19 cases in the country would decrease by the end of August. Furthermore, our findings indicated that relaxing travel restrictions from a highly vaccinated region to a low vaccinated region would result in an epidemic outbreak.
    Matched MeSH terms: Vaccination/methods
  4. Garton K, Kraak V, Fanzo J, Sacks G, Vandevijvere S, Haddad L, et al.
    Public Health Nutr, 2022 Sep;25(9):2353-2357.
    PMID: 35570707 DOI: 10.1017/S1368980022001173
    There is widespread agreement among experts that a fundamental reorientation of global, regional, national and local food systems is needed to achieve the UN Sustainable Development Goals Agenda and address the linked challenges of undernutrition, obesity and climate change described as the Global Syndemic. Recognising the urgency of this imperative, a wide range of global stakeholders - governments, civil society, academia, agri-food industry, business leaders and donors - convened at the September 2021 UN Food Systems Summit to coordinate numerous statements, commitments and declarations for action to transform food systems. As the dust settles, how will they be pieced together, how will governments and food corporations be held to account and by whom? New data, analytical methods and global coalitions have created an opportunity and a need for those working in food systems monitoring to scale up and connect their efforts in order to inform and strengthen accountability actions for food systems. To this end, we present - and encourage stakeholders to join or support - an Accountability Pact to catalyse an evidence-informed transformation of current food systems to promote human and ecological health and wellbeing, social equity and economic prosperity.
    Matched MeSH terms: Food Industry/methods
  5. Al-Amshawee SKA, Yunus MYBM
    Environ Res, 2023 Feb 15;219:115115.
    PMID: 36574794 DOI: 10.1016/j.envres.2022.115115
    The incorporation of a spacer among membranes has a major influence on fluid dynamics and performance metrics. Spacers create feed channels and operate as turbulence promoters to increase mixing and reduce concentration/temperature polarization effects. However, spacer geometry remains unoptimized, and studies continue to investigate a wide range of commercial and custom-made spacer designs. The in-depth discussion of the present systematic review seeks to discover the influence of Reynolds number or solution flowrate on flow hydrodynamics throughout a spacer-filled channel. A fast-flowing solution sweeping one membrane's surface first, then the neighboring membrane's surface produces good mixing action, which does not happen commonly at laminar solution flowrates. A sufficient flowrate can suppress the polarization layer, which may normally require the utilization of a simple feed channel rather than complex spacer configurations. When a recirculation eddy occurs, it disrupts the continuous flow and effectively curves the linear fluid courses. The higher the flowrate, the better the membrane performance, the higher the critical flux (or recovery rate), and the lower the inherent limitations of spacer design, spacer shadow effect, poor channel hydrodynamics, and high concentration polarization. In fact, critical flow achieves an acceptable balance between improving flow dynamics and reducing the related trade-offs, such as pressure losses and the occurrence of concentration polarization throughout the cell. If the necessary technical flowrate is not used, the real concentration potential for transport is relatively limited at low velocities than would be predicted based on bulk concentrations. Electrodialysis stack therefore may suffer from the dissociation of water molecules. Next studies should consider that applying a higher flowrate results in greater process efficiency, increased mass transfer potential at the membrane interface, and reduced stack thermal and electrical resistance, where pressure drop should always be indicated as a consequence of the spacer and circumstances used, rather than a problem.
    Matched MeSH terms: Electrochemistry/methods
  6. Kam CA
    Singapore Med J, 1978 Jun;19(2):106-8.
    PMID: 751183
    A system of tutorials preparing students for the Primary F.F.A.R.A.C.S. examination is described. It is suggested that this system would be suitable for teaching students in a peripheral training hospital.
    Matched MeSH terms: Teaching/methods*
  7. Begum M, Masud MM, Alam L, Mokhtar MB, Amir AA
    Environ Sci Pollut Res Int, 2022 Dec;29(58):87923-87937.
    PMID: 35819668 DOI: 10.1007/s11356-022-21845-z
    Several studies have highlighted the significant impact of climate change on agriculture. However, there have been little empirical enquiries into the impact of climate change on marine fish production, particularly in Bangladesh. Hence, this study aims to investigate the impact of climate change on marine fish production in Bangladesh using data from 1961 to 2019. Data were obtained from the Food and Agriculture Organization, Bangladesh Meteorological Department, the World Development Indicators, and the National Oceanic and Atmospheric Administration. The autoregressive distributed lag (ARDL) model was used to describe the dynamic link between CO2 emissions, average temperature, Sea Surface Temperature (SST), rainfall, sunshine, wind and marine fish production. The ARDL approach to cointegration revealed that SST (β = 0.258), rainfall (β =0.297), and sunshine (β =0.663) significantly influence marine fish production at 1% and 10% levels in the short run and at 1% level in the long run. The results also found that average temperature has a significant negative impact on fish production in both short and long runs. On the other hand, CO2 emissions have a negative impact on marine fish production in the short run. Specifically, for every 1% rise in CO2 emissions, marine fish production will decline by 0.11%. The findings of this study suggest that policymakers formulate better policy frameworks for climate change adaptation and sustainable management of marine fisheries at the national level. Research and development in Bangladesh's fisheries sector should also focus on marine fish species that can resist high sea surface temperatures, CO2 emissions, and average temperatures.
    Matched MeSH terms: Agriculture/methods
  8. Khare SK, Bajaj V, Acharya UR
    Physiol Meas, 2023 Mar 08;44(3).
    PMID: 36787641 DOI: 10.1088/1361-6579/acbc06
    Objective.Schizophrenia (SZ) is a severe chronic illness characterized by delusions, cognitive dysfunctions, and hallucinations that impact feelings, behaviour, and thinking. Timely detection and treatment of SZ are necessary to avoid long-term consequences. Electroencephalogram (EEG) signals are one form of a biomarker that can reveal hidden changes in the brain during SZ. However, the EEG signals are non-stationary in nature with low amplitude. Therefore, extracting the hidden information from the EEG signals is challenging.Approach.The time-frequency domain is crucial for the automatic detection of SZ. Therefore, this paper presents the SchizoNET model combining the Margenau-Hill time-frequency distribution (MH-TFD) and convolutional neural network (CNN). The instantaneous information of EEG signals is captured in the time-frequency domain using MH-TFD. The time-frequency amplitude is converted to two-dimensional plots and fed to the developed CNN model.Results.The SchizoNET model is developed using three different validation techniques, including holdout, five-fold cross-validation, and ten-fold cross-validation techniques using three separate public SZ datasets (Dataset 1, 2, and 3). The proposed model achieved an accuracy of 97.4%, 99.74%, and 96.35% on Dataset 1 (adolescents: 45 SZ and 39 HC subjects), Dataset 2 (adults: 14 SZ and 14 HC subjects), and Dataset 3 (adults: 49 SZ and 32 HC subjects), respectively. We have also evaluated six performance parameters and the area under the curve to evaluate the performance of our developed model.Significance.The SchizoNET is robust, effective, and accurate, as it performed better than the state-of-the-art techniques. To the best of our knowledge, this is the first work to explore three publicly available EEG datasets for the automated detection of SZ. Our SchizoNET model can help neurologists detect the SZ in various scenarios.
    Matched MeSH terms: Electroencephalography/methods
  9. Athmalingam VKR, Wong KW
    Semin Dial, 2022 May;35(3):245-250.
    PMID: 34390274 DOI: 10.1111/sdi.13012
    BACKGROUND: Tenckhoff catheter (TC) insertion for patients with previous intraperitoneal surgery (IPS) is challenging.

    METHODS: This is a case series of 11 patients with history of previous IPS who underwent TC insertion under combined fluoroscopic and sonographic (CFS) guidance with preperitoneal tunneling at our center.

    RESULTS: This is an interim result of our study. The mean age of the patients was 49.1 (±12.7). Seven were females, and four were males. Only two patients underwent more than one IPS prior to this procedure. The mean body mass index (BMI) of patients was 29.2 kg/m2 (±6.2). All patients underwent the procedure successfully. One patient developed post-procedure exit site bleeding which resolved spontaneously. One patient had urgent-start peritoneal dialysis (PD) (less than 72 h), and two patients had early-start PD (less than 2 weeks). Median catheter survival is 8 months at the time of writing.

    CONCLUSION: CFS-assisted TC insertion with preperitoneal tunneling for patients with previous IPS is a safe and effective technique.

    Matched MeSH terms: Catheterization/methods
  10. Meng X, Wen K, Citartan M, Lin Q
    Analyst, 2023 Feb 13;148(4):787-798.
    PMID: 36688616 DOI: 10.1039/d2an01767a
    Aptamers are single-stranded oligonucleotide molecules that bind with high affinity and specificity to a wide range of target molecules. The method of systematic evolution of ligands by exponential enrichment (SELEX) plays an essential role in the isolation of aptamers from a randomized oligonucleotide library. To date, significant modifications and improvements of the SELEX process have been achieved, engendering various forms of SELEX from conventional SELEX to microfluidics-based full-chip SELEX. While full-chip SELEX is generally considered advantageous over conventional SELEX, there has not yet been a conclusive comparison between the methods. Herein, we present a comparative study of three SELEX strategies for aptamer isolation, including those using conventional agarose bead-based partitioning, microfluidic affinity selection, and fully integrated microfluidic affinity selection and PCR amplification. Using immunoglobulin E (IgE) as a model target molecule, we compare these strategies in terms of the time and cost for each step of the SELEX process including affinity selection, amplification, and oligonucleotide conditioning. Target-binding oligonucleotides in the enriched pools are sequenced and compared to assess the relative efficacy of the SELEX strategies. We show that the microfluidic strategies are more time- and cost-efficient than conventional SELEX.
    Matched MeSH terms: SELEX Aptamer Technique/methods
  11. Low TY, Lee PY
    Methods Mol Biol, 2023;2690:69-80.
    PMID: 37450137 DOI: 10.1007/978-1-0716-3327-4_6
    Proteins often interact with each other to form complexes and play functional roles in almost all cellular processes. The study of protein-protein interactions is therefore critical to understand protein function and biological pathways. Affinity Purification coupled with Mass Spectrometry (AP-MS) is an invaluable technique for identifying the interaction partners in protein complexes. In this approach, the protein of interest is fused to an affinity tag, followed by the expression and purification of the fusion protein. The affinity-purified sample is then analyzed by mass spectrometry to identify the interaction partners of the bait proteins. In this chapter, we detail the protocol for tandem affinity purification (TAP) based on the use of the FLAG (a fusion tag with peptide sequence DYKDDDDK) and hemagglutinin (HA) peptide epitopes. The immunoprecipitation using dual-affinity tags offers the advantage of increasing the specificity of the purification with lower nonspecific-background interactions.
    Matched MeSH terms: Chromatography, Affinity/methods
  12. Kundu M, Ng JC, Awuah WA, Huang H, Yarlagadda R, Mehta A, et al.
    Postgrad Med J, 2023 May 22;99(1170):240-243.
    PMID: 36892407 DOI: 10.1093/postmj/qgad002
    The tremendous evolution in modern technology has led to a paradigm shift in neurosurgery. The latest advancements such as augmented reality, virtual reality, and mobile applications have been incorporated into neurosurgical practice. NeuroVerse, representing the application of the metaverse in neurosurgery, brings enormous potential to neurology and neurosurgery. Implementation of NeuroVerse could potentially elevate neurosurgical and interventional procedures, enhance medical visits and patient care, and reshape neurosurgical training. However, it is also vital to consider the challenges that may be associated with its implementation, such as privacy issues, cybersecurity breaches, ethical concerns, and widening of existing healthcare inequalities. NeuroVerse adds phenomenal dimensions to the neurosurgical environment for patients, doctors, and trainees, and represents an incomparable advancement in the delivery of medicine. Therefore, more research is needed to encourage widespread use of the metaverse in healthcare, particularly focusing on the areas of morality and credibility. Although the metaverse is expected to expand rapidly during and after the COVID-19 pandemic, it remains to be seen whether it represents an emerging technology that will revolutionize our society and healthcare or simply an immature condition of the future.
    Matched MeSH terms: Neurosurgical Procedures/methods
  13. Gan RK, Sanchez Martinez A, Abu Hasan MA, Castro Delgado R, Arcos González P
    J Ultrasound, 2023 Jun;26(2):343-353.
    PMID: 36694072 DOI: 10.1007/s40477-022-00761-5
    INTRODUCTION: Necrotizing fasciitis (NF) is a rapidly progressive necrosis of the fascial layer with a high mortality rate. It is a life-threatening medical emergency that requires urgent treatment. Lack of skin finding in NF made diagnosis difficult and required a high clinical index of suspicion. The use of ultrasound may guide clinicians in improving diagnostic speed and accuracy, thus leading to improved management decisions and patient outcomes. This literature search aims to review the use of point-of-care ultrasonography in diagnosing necrotizing fasciitis.

    METHOD: We searched relevant electronic databases, including PUBMED, MEDLINE, and SCOPUS, and performed a systematic review. Keywords used were "necrotizing fasciitis" or "necrotising fasciitis" or "necrotizing soft tissue infections" and "point-of-care ultrasonography" "ultrasonography" or "ultrasound". No temporal limitation was set. An additional search was performed via google scholar, and the top 100 entry was screened.

    RESULTS: Among 540 papers screened, only 21 were related to diagnosing necrotizing fasciitis using ultrasonography. The outcome includes three observational studies, 16 case reports, and two case series, covering the period from 1976 to 2022.

    CONCLUSION: Although the use of ultrasonography in diagnosing NF was published in several papers with promising results, more studies are required to investigate its diagnostic accuracy and potential to reduce time delay before surgical intervention, morbidity, and mortality.

    Matched MeSH terms: Ultrasonography/methods
  14. Lee JM, Wasserman RJ, Wilson RF, Cuthbert RN, Rahman S, Yek SH
    Ecohealth, 2023 Mar;20(1):65-73.
    PMID: 37129695 DOI: 10.1007/s10393-023-01629-8
    Fogging with insecticides is one of the main control measures for adult mosquito populations employed in countries that are affected by dengue. In many such countries, urban communities are increasingly characterised by high-density residence in high-rise condominia. Although fogging is typically applied at the ground level, its efficacy in three-dimensional urban environments is poorly understood. Here, we investigated the effect of fogging on vector mosquito distribution and abundance in high-rise condominia by conducting a before-after fogging survey. We showed that although mosquitoes were significantly concentrated at the lower levels in high-rise condominia, they were found throughout the three-dimensional environments. Fogging did not significantly alter this distribution or abundance pattern across any floor level. Thus, any fogging effect was short-lived as mosquito populations recovered within a few days before the subsequent scheduled treatment. In addition, increasing fogging frequency within practicable limits did not prolong the intended control effect. As urban mosquitoes are increasingly insusceptible to fogging due to insecticide resistance and vertical avoidance, this study demonstrates the need to implement other mosquito control strategies for high-rise condominia to manage mosquito populations.
    Matched MeSH terms: Mosquito Control/methods
  15. Then SM, Kokolski M, Mbaki Y, Merrick D, Anderson S
    Anat Histol Embryol, 2023 Jan;52(1):21-30.
    PMID: 36373558 DOI: 10.1111/ahe.12888
    Histology is often taught in higher education settings using online virtual microscopes (VM). This study aimed to develop and evaluate the use of VM in teaching on a BSc degree at the University of Nottingham by surveying students and staff. A key development was the use of an e-workbook so that students were actively engaged in creating their own bespoke revision material. Subsequently, this approach was used in a second study evaluating the use of VM in teaching the histology and pathology of the gastrointestinal (GI) tract via group work with students from two BSc courses at the University of Nottingham; one based at Derby (RDHC) and the other in Malaysia (UNMC). Students worked together in groups to complete an e-workbook, develop a presentation, and decide how to collaborate and communicate. An evaluation of these activities revealed advantages in developing transferrable skills, and good engagement with both the histology topic and group work. Analysis of assessment of the module at UNMC showed that student performance improved in the histology-based module after the intervention (p 
    Matched MeSH terms: Microscopy/methods
  16. Wimalasiri EM, Ashfold MJ, Jahanshiri E, Walker S, Azam-Ali SN, Karunaratne AS
    PLoS One, 2023;18(3):e0283298.
    PMID: 36952502 DOI: 10.1371/journal.pone.0283298
    Current agricultural production depends on very limited species grown as monocultures that are highly vulnerable to climate change, presenting a threat to the sustainability of agri-food systems. However, many hundreds of neglected crop species have the potential to cater to the challenges of climate change by means of resilience to adverse climate conditions. Proso millet (Panicum miliaceum L.), one of the underutilised minor millets grown as a rainfed subsistence crop, was selected in this study as an exemplary climate-resilient crop. Using a previously calibrated version of the Agricultural Production Systems Simulator (APSIM), the sensitivity of the crop to changes in temperature and precipitation was studied using the protocol of the Coordinated Climate Crop Modelling Project (C3MP). The future (2040-2069) production was simulated using bias-corrected climate data from 20 general circulation models of the Coupled Model Intercomparison Project (CMIP5) under RCP4.5 and 8.5 scenarios. According to the C3MP analysis, we found a 1°C increment of temperature decreased the yield by 5-10% at zero rainfall change. However, Proso millet yields increased by 5% within a restricted climate change space of up to 2°C of warming with increased rainfall. Simulated future climate yields were lower than the simulated yields under the baseline climate of the 1980-2009 period (mean 1707 kg ha-1) under both RCP4.5 (-7.3%) and RCP8.5 (-16.6%) though these changes were not significantly (p > 0.05) different from the baseline yields. Proso millet is currently cultivated in limited areas of Sri Lanka, but our yield mapping shows the potential for expansion of the crop to new areas under both current and future climates. The results of the study, indicating minor impacts from projected climate change, reveal that Proso millet is an excellent candidate for low-input farming systems under changing climate. More generally, through this study, a framework that can be used to assess the climate sensitivity of underutilized crops was also developed.
    Matched MeSH terms: Agriculture/methods
  17. Concepcion NDP, Laya BF, Andronikou S, Abdul Manaf Z, Atienza MIM, Sodhi KS
    Pediatr Radiol, 2023 Aug;53(9):1773-1781.
    PMID: 37081179 DOI: 10.1007/s00247-023-05654-1
    Tuberculosis (TB) remains a global health problem and is the second leading cause of death from a single infectious agent, behind the novel coronavirus disease of 2019. Children are amongst the most vulnerable groups affected by TB, and imaging manifestations are different in children when compared to adults. TB primarily involves the lungs and mediastinal lymph nodes. Clinical history, physical examination, laboratory examinations and various medical imaging tools are combined to establish the diagnosis. Even though chest radiography is the accepted initial radiological imaging modality for the evaluation of children with TB, this paper, the first of two parts, aims to discuss the advantages and limitations of the various medical imaging modalities and to provide recommendations on which is most appropriate for the initial diagnosis and assessment of possible complications of pulmonary TB in children. Practical, evidence-based imaging algorithms are also presented.
    Matched MeSH terms: Radiography, Thoracic/methods
  18. Imran M, Ahmed S, Abdullah AZ, Hakami J, Chaudhary AA, Rudayni HA, et al.
    Luminescence, 2023 Jul;38(7):1064-1086.
    PMID: 36378274 DOI: 10.1002/bio.4408
    The penicillin derivative amoxicillin (AMX) plays an important role in treating various types of infections caused by bacteria. However, excessive use of AMX may have negative health effects. Therefore, it is of utmost importance to detect and quantify the AMX in pharmaceutical drugs, biological fluids, and environmental samples with high sensitivity. Therefore, this review article provides valuable and up-to-date information on nanostructured material-based optical and electrochemical sensors to detect AMX in various biological and chemical samples. The role of using different nanostructured materials on the performance of important optical sensors such as colorimetric sensors, fluorescence sensors, surface-enhanced Raman scattering sensors, chemiluminescence/electroluminescence sensors, optical immunosensors, optical fibre-based sensors, and several important electrochemical sensors based on different electrode types have been discussed. Moreover, nanocomposites, polymer, and MXenes-based electrochemical sensors have also been discussed, in which such materials are being used to further enhance the sensitivity of these sensors. Furthermore, nanocomposite-based photo-electrochemical sensors and the market availability of biosensors including AMX have also been discussed briefly. Finally, the conclusion, challenges, and future perspectives of the above-mentioned sensing techniques for AMX detection are presented.
    Matched MeSH terms: Electrochemical Techniques/methods
  19. Yusefi M, Shameli K, Jahangirian H, Teow SY, Afsah-Hejri L, Mohamad Sukri SNA, et al.
    Int J Nanomedicine, 2023;18:3535-3575.
    PMID: 37409027 DOI: 10.2147/IJN.S375964
    Chemotherapy is the most prominent route in cancer therapy for prolonging the lifespan of cancer patients. However, its non-target specificity and the resulting off-target cytotoxicities have been reported. Recent in vitro and in vivo studies using magnetic nanocomposites (MNCs) for magnetothermal chemotherapy may potentially improve the therapeutic outcome by increasing the target selectivity. In this review, magnetic hyperthermia therapy and magnetic targeting using drug-loaded MNCs are revisited, focusing on magnetism, the fabrication and structures of magnetic nanoparticles, surface modifications, biocompatible coating, shape, size, and other important physicochemical properties of MNCs, along with the parameters of the hyperthermia therapy and external magnetic field. Due to the limited drug-loading capacity and low biocompatibility, the use of magnetic nanoparticles (MNPs) as drug delivery system has lost traction. In contrast, MNCs show higher biocompatibility, multifunctional physicochemical properties, high drug encapsulation, and multi-stages of controlled release for localized synergistic chemo-thermotherapy. Further, combining various forms of magnetic cores and pH-sensitive coating agents can generate a more robust pH, magneto, and thermo-responsive drug delivery system. Thus, MNCs are ideal candidate as smart and remotely guided drug delivery system due to a) their magneto effects and guide-ability by the external magnetic fields, b) on-demand drug release performance, and c) thermo-chemosensitization under an applied alternating magnetic field where the tumor is selectively incinerated without harming surrounding non-tumor tissues. Given the important effects of synthesis methods, surface modifications, and coating of MNCs on their anticancer properties, we reviewed the most recent studies on magnetic hyperthermia, targeted drug delivery systems in cancer therapy, and magnetothermal chemotherapy to provide insights on the current development of MNC-based anticancer nanocarrier.
    Matched MeSH terms: Drug Delivery Systems/methods
  20. Nguyen TA, Kirubakaran R, Schultz HB, Wong S, Reuter SE, McMullan B, et al.
    Clin Chem, 2023 Jun 01;69(6):637-648.
    PMID: 37116191 DOI: 10.1093/clinchem/hvad036
    BACKGROUND: Therapeutic drug monitoring (TDM) of aminoglycosides and vancomycin is used to prevent oto- and nephrotoxicity in neonates. Analytical and nonanalytical factors potentially influence dosing recommendations. This study aimed to determine the impact of analytical variation (imprecision and bias) and nonanalytical factors (accuracy of drug administration time, use of non-trough concentrations, biological variation, and dosing errors) on neonatal antimicrobial dosing recommendations.

    METHODS: Published population pharmacokinetic models and the Australasian Neonatal Medicines Formulary were used to simulate antimicrobial concentration-time profiles in a virtual neonate population. Laboratory quality assurance data were used to quantify analytical variation in antimicrobial measurement methods used in clinical practice. Guideline-informed dosing recommendations based on drug concentrations were applied to compare the impact of analytical variation and nonanalytical factors on antimicrobial dosing.

    RESULTS: Analytical variation caused differences in subsequent guideline-informed dosing recommendations in 9.3-12.1% (amikacin), 16.2-19.0% (tobramycin), 12.2-45.8% (gentamicin), and 9.6-19.5% (vancomycin) of neonates. For vancomycin, inaccuracies in drug administration time (45.6%), use of non-trough concentrations (44.7%), within-subject biological variation (38.2%), and dosing errors (27.5%) were predicted to result in more dosing discrepancies than analytical variation (12.5%). Using current analytical performance specifications, tolerated dosing discrepancies would be up to 14.8% (aminoglycosides) and 23.7% (vancomycin).

    CONCLUSIONS: Although analytical variation can influence neonatal antimicrobial dosing recommendations, nonanalytical factors are more influential. These result in substantial variation in subsequent dosing of antimicrobials, risking inadvertent under- or overexposure. Harmonization of measurement methods and improved patient management systems may reduce the impact of analytical and nonanalytical factors on neonatal antimicrobial dosing.

    Matched MeSH terms: Drug Monitoring/methods
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