Displaying publications 21 - 40 of 137 in total

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  1. Mohamed R, Yip C, Singh S
    Eur J Gastroenterol Hepatol, 2023 Jul 01;35(7):742-752.
    PMID: 37161976 DOI: 10.1097/MEG.0000000000002548
    BACKGROUND: Viral hepatitis B and C (HBV, HCV) and non-alcoholic fatty liver disease (NAFLD) are the commonest etiologies of liver-related deaths in Malaysia. Herein, this study aims to explore and understand the knowledge and attitudes of the public toward liver-related health and diseases in Malaysia.

    METHODS: A cross-sectional, self-reported, web-based questionnaire was conducted among 500 adults between February and March 2020. Questionnaire items pertained to the knowledge and attitudes toward liver-related health and diseases.

    RESULTS: Half of the respondents were aged ≥35 years and 52.0% were males. Gaps in knowledge included the lack of awareness of different types of hepatitis, including the potential transmission risks and complications of HBV and HCV. About half acknowledged liver fibrosis and cirrhosis as key determinants of liver-related disease progression. A higher proportion rightly recognized the diagnostic test for HCV (40.8%) than HBV (30.0%) despite more being aware of HBV than HCV. Less than one-third were aware of the risk factors, screening tests, and complications of NAFLD. Despite the majority (92.8%) agreeing that regular screening was important for liver health, only 67.0% attended recent health screening and one-fifth were unlikely to seek medical consultation upon exposure to viral hepatitis risk factors. Reasons for this low urgency included the perception of being healthy, cost-related concerns, and societal discrimination.

    CONCLUSION: Robust education efforts are needed to raise awareness and empower the community with knowledge of liver-related diseases, particularly viral hepatitis and NAFLD in Malaysia.

  2. Wan Manan W.M., Noor Izani N.J., Nik Nurain N.H., Razlina A.R., Nik Rosmawati N.H.
    JUMMEC, 2018;21(1):21-27.
    MyJurnal
    Background: School children are at great risk of nutrition-related chronic diseases. Their eating pattern in
    school is influenced by the availability of food served within the school environment. The purpose of this
    paper was to determine the effectiveness of a canteen-based food nutrition intervention, designed according
    to the theory of planned behaviour, on the knowledge, perception and choices of healthy food among primary
    schoolchildren.
    Methods: This was a prospective intervention study using a two-group design. Six school canteens from the
    ninety-eight primary schools were randomly allocated to an intervention group or a control group. All the
    food handlers in the intervention group were given a twelve-week training course in food nutrition. On the
    completion of the course, 293 Standard Six students from these schools, were interviewed with a nutritional
    questionnaire on food in their school canteens. The training programme for the intervention group and the
    questionnaire for the students were developed and validated for the study, and a power calculation made for
    the sample size of students.
    Results: The students in both groups were homogeneous in gender and body mass. The mean body mass
    index (BMI) was 19.26 (SD: 0.38) and 19.47 (SD: 0.39) for the intervention and the control group respectively.
    There were more schoolchildren in the intervention group who purchased foods in school canteens (p 0.0036)
    of milk and milk products (p 0.034), and white rice (p
  3. Siti Aishah Muhadi, Habsah Hasan, Nurahan Maning, Nik Rosmawati Nik Husain, Fatimah Haslina Abdullah
    MyJurnal
    Introduction: HIV is the leading cause of mortality and morbidity worldwide. There are two types of HIV, HIV-1, and HIV-2, which are geographically different in epidemiology and determinants. Objective: To determine the epidemiology and determinants of HIV-1 and HIV-1&2 in the three tertiary hospitals of Eastern Peninsular Malaysia. Method: A cross-sectional study of confirmed serologically HIV-1 and HIV-1&2 from January 2016 until December 2018. SPSS analysed all collected data, descriptive statistics for sociodemographic data and Pearson chi-square for the association between type of HIV with HCV, HBV, syphilis, and tuberculosis. In identifying the risk factor associated with HIV-1&2, several variables were tested by the Multiple Logistic Regression Model. A P-value of
  4. Nik Nor Ronaidi, N.M., Mohd, N.S., Wan Mohammad, Z., Sharina, D., Nik Rosmawati, N.H.
    MyJurnal
    Treating tuberculosis (TB) remains a public health challenge in many developing countries. Treatment success rate in Malaysia is on declining pattern from 78% in 2000 to 48% in 2006. This study will determine factors associated with unsuccessful treatment among pulmonary TB patients. A retrospective cohort study was conducted on notified and treated pulmonary TB cases in Kota Bharu district Kelantan between January 2006 and December 2007. To identify the associated factors, univariate comparison and multiple logistic regressions were performed. Among the 765 patients identified, 472 (61.7%) cases fulfilled the criteria and were analyzed. There were 316 males and 156 females and their mean (SD) age was 45 (17.9) years. In univariable analysis, age, gender, educational level, employment status, family incomes as well as coexistence of extra-pulmonary TB, smoking, co-morbid disease (diabetes mellitus), HIV status, sputum cultures, chest X-ray findings and duration of delay for diagnosis were all found to have significant relationship with unsuccessful pulmonary TB treatment outcome. After adjusted for the confounders, the significant predictors for unsuccessful tuberculosis treatment were age (Adj. OR 1.09; 95% CI: 1.03-1.15), HIV positive (Adj. OR 23.04; 95% CI: 3.01-176.22) and advanced chest X-ray findings (Adj. OR 6.51; 95% CI: 1.50-28.23). A large proportion of unsuccessfully treated cases could be identified at entry
    by screening for age, chest X-rays and HIV status and specially targeted measures could be taken. The use of directly observed treatment short-course (DOTS) should be given more focus and priority among this high risk group of patients.
  5. Nur Syakilah Mahyiddin, Rosmawati Mohamed, Hamid Jan Jan Mohamed, Norfarhana Ramly
    MyJurnal
    Dengue incidence has grown dramatically in recent decades and become a global incidence especially in the
    urban area. Ministry of Health (MOH) Malaysia reported that the number of dengue cases since January
    2014 until June 2014 was 38. 411 cases which was higher compared to the number reported in year 2013 (10,
    910 cases). The objective of the study was to determine the knowledge and preventive practices of dengue
    among residents in Flat Pandan Indah, Ampang Selangor. A cross sectional study design with convenience
    sampling method was adopted in this study. A total of 298 respondents living in Flat Pandan Indah were
    recruited based on the inclusion criteria. A validated questionnaire that measures knowledge and preventive
    practices of dengue was completed by respondents. More than half of the respondents had scored 61.7%
    with good knowledge regarding dengue. However, 50.3% of the respondents had a limited extent of dengue
    practice. Almost all of the respondents (95.6%) reported that television or radio as the main source for
    information on dengue. The common preventive measures used by the respondents were cleaning the
    house (n=148, 49.7%), eliminating standing water around the house (n=123, 41.3%) and covering water
    containers in the home (n=120, 40.a3%). There was no correlation between dengue knowledge and
    preventive practice score (p = 0.109). The study revealed that majority of the respondents had good
    knowledge regarding dengue but the practices to prevent dengue are poor. Thus, health education program
    focusing on dengue prevention practices should be done on the community members in the future.
  6. Nik Rosmawati N, Wan Manan W, Noor Izani N, Nik Nurain N, Razlina A
    Sains Malaysiana, 2018;47:2119-2128.
    This study aims to assess the effectiveness of a newly developed food safety education to improve the handwashing
    practices of food handlers in school canteens. A community-based intervention study was carried out over a 2-year period.
    Sixteen out of 98 primary schools were randomly selected and assigned into intervention and control groups using a
    simple random sampling method. The study population included food handlers who worked in the canteens of the school
    selected. The Food Safety Education Programme (FSEP) for the intervention group was developed based on the theory of
    planned behaviour. The main outcome measures used were handwashing practices from the observations carried out at
    baseline, 6-weeks (Post1) and 12-weeks (Post2) after the intervention. Out of 79 food handlers who participated in this
    study, 33 (41.8%) were in the intervention group and 46 (58.2%) were in the control group. Prior to FSEP, handwashing
    was not commonly practiced following critical events and the majority did not perform correctly. The time-effect of the
    mixed design analysis of variance showed a significant increase (p=0.004) in the mean percentage of the total observed
    handwashing practices from 29% at the baseline to 50.8% at Post1 (p=0.004). However, the intervention-effect of mixed
    design ANOVA did not show any significant difference in the handwashing practices (p=0.210). The FSEP was effective in
    improving the handwashing practices of the food handlers in the selected primary school canteens.
  7. Nik Rosmawati, N.H., Wan Manan, W.M., Noor Izani, N.J., Nik Nurain, N.H., Razlina, A.R.
    MyJurnal
    The aim of this study was to evaluate the impact of Food Nutrition Intervention (FNI) for
    improving food handlers’ knowledge and serving of competitive food in the primary school
    canteens. We randomized 16 out of 98 primary schools into intervention and control groups
    using a multistage sampling method. The training programme for the intervention group and
    questionnaires for evaluating knowledge were developed. On-site observations were done to
    document all competitive foods served in school canteens. Out of the 79 food handlers who
    participated in this study, 33 (41.8%) were in the intervention group and 46 (58.2%) were
    in the control group. The majority of food handlers were female, Malay, had education at
    middle levels, and at middle-aged. The commonest food category served was carbohydrate
    (75%), high-fat foods (34.4%) and food not recommended for sale (34.4%). Knowledge about
    healthy food choice in the intervention group at 6 weeks and 12 weeks post intervention were
    significantly higher than at baseline. The intervention group also demonstrated significantly
    better knowledge composite score at 6 weeks and 12 weeks following intervention. These
    improvements were also observed between intervention and control regardless of time. By
    six weeks, the number of vegetable menu served significantly increased in the intervention
    (p=0.040) and by 12 weeks, the number of milk and milk products served also significantly
    increased (p=0.015) as compared to the control group. Thus, the provision of FNI was
    associated with significantly improved healthy food knowledge amongst food handlers, the
    serving of vegetables and milk or milk products in school canteens.
  8. Muhammad J, Muhamad R, Husain NRN, Daud N
    Korean J Fam Med, 2019 Jan;40(1):45-52.
    PMID: 30613064 DOI: 10.4082/kjfm.17.0136
    BACKGROUND: Pelvic floor muscle exercise is effective for pelvic floor muscle dysfunction. Despite the high prevalence of pelvic floor muscle dysfunction in women in the community, pelvic floor muscle exercise recognition is low in Malaysian society; thus, this intervention is not frequently used . This study assessed the knowledge of, attitudes towards, and use of pelvic floor muscle exercise among antenatal women before and after educational classes. The study also determined factors associated with exercise practice.

    METHODS: An interventional study was conducted in 121 antenatal women selected through systematic random sampling between June and December 2010. Baseline knowledge of, attitude towards, and practice of pelvic floor muscle exercise were assessed using self-administered validated questionnaires at first visit and at 2 months postpartum. All participants attended two exercise education classes on their next two visits before delivery. A paired ttest and multivariate analysis were used for data evaluation.

    RESULTS: The mean pre-intervention scores for knowledge, attitudes, and practice were 24.98, 24.25, and 3.51, respectively, with statistically significant mean score increments after intervention (P<0.001). The mean differences were 4.67 (95% confidence interval [CI], 3.86-5.49), 3.77 (95% CI, 3.05-4.50) and 3.45 (95% CI, 2.90-4.00) for knowledge, attitudes, and practice, respectively. Lack of baseline information on pelvic floor muscle exercise was significantly associated with practice change following an educational class.

    CONCLUSION: Education is effective in improving knowledge of, attitude towards, and practice of pelvic floor muscle exercise. There is a need for greater effort to increase exercise awareness in our community, especially during antenatal class.

  9. Jikal M, Riduan T, Aarifin R, Jeffree MS, Ahmed K
    Int J Infect Dis, 2019 Jun;83:83-85.
    PMID: 30986543 DOI: 10.1016/j.ijid.2019.04.008
    OBJECTIVES: In this study we investigated an outbreak of Vibrio cholera O1 Ogawa serotype, occurred during December 2014 in Kudat district, situated in Sabah state of the Malaysian part of Borneo.

    METHODS: Active case detection and contact tracing were done at respective localities by house to house survey. Passive case detection was done among acute gastroenteritis patients attended at various health facilities. To determine the source, samples from food, water and environment were taken. A case control study was also done to determine the risk factors.

    RESULTS: A total of 44 symptomatic and 34 asymptomatic cases from 19 localities were investigated. 39 cases were detected through passive case detection. Median age of cases was 23 years. All cases belonged to serogroup O1 and Ogawa serotype. The epidemiological investigation of time, place, and person identified that V. cholerae cross-transmission might have occurred in two fish markets and the fish-loading port. Circumstantial evidences indicated that cholera was possibly transmitted through contaminated sea foods.

    CONCLUSIONS: We concluded that the life-style of Sea Gypsies is a challenge in cholera control; therefore vaccination might be an effective way to mitigate cholera in an outbreak prone area like Kudat.

  10. Riazalhosseini B, Mohamed Z, Apalasamy YD, Eng HS, Mohamed R
    Pharmacogenet Genomics, 2016 Feb;26(2):74-9.
    PMID: 26529280 DOI: 10.1097/FPC.0000000000000187
    MicroRNAs (miRNAs) are small noncoding RNAs that have been implicated in mechanisms underlying various types of cancers including hepatocellular carcinoma (HCC). Reports have indicated that single nucleotide polymorphisms in miRNA-196A2 and miRNA-146A genes may contribute to the risk of progression of hepatitis B virus (HBV) infection to cirrhosis and HCC. This study aimed to examine the effect of miRNA-196A2 and miRNA-146A polymorphisms on the progression of HBV infection to cirrhosis and/or HCC in HBV patients in the Malaysian population.
  11. Tan HL, Mohamed R, Mohamed Z, Zain SM
    Pharmacogenet Genomics, 2016 Feb;26(2):88-95.
    PMID: 26636496 DOI: 10.1097/FPC.0000000000000193
    Phosphatidylethanolamine N-methyltransferase (PEMT) governs the secretion of hepatic triglycerides in the form of very low-density lipoprotein and has been implicated in nonalcoholic fatty liver disease (NAFLD). Studies on the role of the PEMT rs7946 polymorphism as a genetic modifier of NAFLD have reported inconsistent results. This meta-analysis was carried out to evaluate and summarize the association of PEMT rs7946 with susceptibility to NAFLD.
  12. Ooi A, Tan S, Mohamed R, Rahman NA, Othman RY
    J Biotechnol, 2006 Feb 24;121(4):471-81.
    PMID: 16271415
    A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.
  13. Riazalhosseini B, Mohamed R, Apalasamy YD, Langmia IM, Mohamed Z
    Rev Soc Bras Med Trop, 2017 Mar-Apr;50(2):161-166.
    PMID: 28562750 DOI: 10.1590/0037-8682-0416-2016
    INTRODUCTION: Hepatitis B virus (HBV) constitutes an important risk factor for cirrhosis and hepatocellular carcinoma (HCC). The link between circulating microRNAs and HBV has been previously reported, although not as a marker of liver disease progression in chronic hepatitis B (CHB). The aim of this study was to characterize miRNA expression profiles between CHB with and without cirrhosis or HCC.

    METHODS:: A total of 12 subjects were recruited in this study. We employed an Affymetrix Gene Chip miRNA 3.0 Array to provide universal miRNA coverage. We compared microRNA expression profiles between CHB with and without cirrhosis/HCC to discover possible prognostic markers associated with the progression of CHB.

    RESULTS:: Our results indicated 8 differently expressed microRNAs, of which miRNA-935, miRNA-342, miRNA-339, miRNA-4508, miRNA-3615, and miRNA-3200 were up-regulated, whereas miRNA-182 and miRNA-4485 were down-regulated in patients with CHB who progressed to cirrhosis/HCC as compared to those without progression.

    CONCLUSIONS:: We demonstrated the differential expression of miRNA-935, miRNA-342, miRNA-339, miRNA-4508, miRNA-3615, miRNA-3200, miRNA-182, and miRNA-4485 between patients with HBV without cirrhosis/HCC and those who had progressed to these more severe conditions. These miRNAs may serve as novel and non-invasive prognostic markers for early detection of CHB-infected patients who are at risk of progression to cirrhosis and/or HCC.
  14. Raihan R, Azzeri A, H Shabaruddin F, Mohamed R
    Euroasian J Hepatogastroenterol, 2018 05 01;8(1):54-56.
    PMID: 29963463 DOI: 10.5005/jp-journals-10018-1259
    Hepatocellular carcinoma (HCC) is one of the leading causes of death globally. In Malaysia liver cancer is the eighth most common cause of cancer for both gender and fifth most common cause of cancer for males. Liver cancer is a cause of premature death in Malaysia: The trend from 1990 to 2010 was observed upward. Since 1990, the annual years of life lost (YLLs) from liver cancer have increased by 31.5%. Older persons are at higher risk and there is male predominance observed. Curative surgical resection, liver transplantation, and supportive symptomatic care, including percutaneous ethanol injection and radiofrequency ablation (RFA), and noncurative transarterial chemoembolization (TACE) are among available treatment facilities. Yet the survival rate is very poor as majority of patients present at very advanced stage. Hepatitis B virus (HBV) remained the leading cause of HCC in Malaysia. Several studies showed cryptogenic causes, which are mainly nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) among the predominant causes of HCC in Malaysia than hepatitis C virus (HCV), alcohol, or any other reason. This mainly correlates with the increasing incidence of diabetes and obesity in Malaysia. How to cite this article: Raihan R, Azzeri A, Shabaruddin FH, Mohamed R. Hepatocellular Carcinoma in Malaysia and Its Changing Trend. Euroasian J Hepato-Gastroenterol 2018;8(1):54-56.
  15. Mohamed R, Shabaruddin FH, Azzeri A, McDonald SA, Dahlui M
    Journal of virus eradication, 2019 Nov 04;5(4):253.
    PMID: 31754450
  16. Masrani AS, Nik Husain NR, Musa KI, Yasin AS
    Biomed Res Int, 2021;2021:3540964.
    PMID: 34734083 DOI: 10.1155/2021/3540964
    Introduction: Dengue, a vector-borne viral illness, shows worldwide widening spatial distribution beyond its point of origination, namely, the tropical belt. The persistent hyperendemicity in Malaysia has resulted in the formation of the dengue early warning system. However, weather variables are yet to be fully utilized for prevention and control activities, particularly in east-coast peninsular Malaysia where limited studies have been conducted. We aim to provide a time-based estimate of possible dengue incidence increase following weather-related changes, thereby highlighting potential dengue outbreaks.

    Method: All serologically confirmed dengue patients in Kelantan, a northeastern state in Malaysia, registered in the eDengue system with an onset of disease from January 2016 to December 2018, were included in the study with the exclusion of duplicate entry. Using a generalized additive model, climate data collected from the Kota Bharu weather station (latitude 6°10'N, longitude 102°18'E) was analysed with dengue data.

    Result: A cyclical pattern of dengue cases was observed with annual peaks coinciding with the intermonsoon period. Our analysis reveals that maximum temperature, mean temperature, rainfall, and wind speed have a significant nonlinear effect on dengue cases in Kelantan. Our model can explain approximately 8.2% of dengue incidence variabilities.

    Conclusion: Weather variables affect nearly 10% of the dengue incidences in Northeast Malaysia, thereby making it a relevant variable to be included in a dengue early warning system. Interventions such as vector control activities targeting the intermonsoon period are recommended.

  17. Masrani AS, Nik Husain NR, Musa KI, Yasin AS
    J Prev Med Public Health, 2022 Jan;55(1):80-87.
    PMID: 35135051 DOI: 10.3961/jpmph.21.461
    OBJECTIVES: Dengue remains hyperendemic in Malaysia despite extensive vector control activities. With dynamic changes in land use, urbanisation and population movement, periodic updates on dengue transmission patterns are crucial to ensure the implementation of effective control strategies. We sought to assess shifts in the trends and spatial patterns of dengue in Kelantan, a north-eastern state of Malaysia (5°15'N 102°0'E).

    METHODS: This study incorporated data from the national dengue monitoring system (eDengue system). Confirmed dengue cases registered in Kelantan with disease onset between January 1, 2016 and December 31, 2018 were included in the study. Yearly changes in dengue incidence were mapped by using ArcGIS. Hotspot analysis was performed using Getis-Ord Gi to track changes in the trends of dengue spatial clustering.

    RESULTS: A total of 10 645 dengue cases were recorded in Kelantan between 2016 and 2018, with an average of 10 dengue cases reported daily (standard deviation, 11.02). Areas with persistently high dengue incidence were seen mainly in the coastal region for the 3-year period. However, the hotspots shifted over time with a gradual dispersion of hotspots to their adjacent districts.

    CONCLUSIONS: A notable shift in the spatial patterns of dengue was observed. We were able to glimpse the shift of dengue from an urban to peri-urban disease with the possible effect of a state-wide population movement that affects dengue transmission.

  18. Raihan R, Mohamed R, Radzi Abu Hassan M, Md Said R
    Euroasian J Hepatogastroenterol, 2017 Jan-Jun;7(1):65-67.
    PMID: 29201775 DOI: 10.5005/jp-journals-10018-1214
    Malaysia is a country where an estimated 1 million people are chronically infected with hepatitis B virus (HBV) and an estimated 2.5% of the adult population are positive for antibody to hepatitis C virus (HCV). Effective nationwide vaccine coverage seems to be a highly effective measure to prevent new HBV infection. Treatment of HCV infection is also a regular practice in Malaysia. These measures highlight the possibility to reach the World Health Organization elimination target by 2030. To achieve this target, the Health Ministry and other nongovernmental organizations, such as My Commitment to Cure (MyC2C) are working together to develop a strategic road map to reach the global elimination target in Malaysia by 2030. How to cite this article: Raihan R, Mohamed R, Hasan MRA, Rosaida MS. Chronic Viral Hepatitis in Malaysia: "Where are we now?" Euroasian J Hepato-Gastroenterol 2017;7(1):65-67.
  19. Ahmad A, Sutanto MH, Ahmad NR, Mohamad ME, Bujang M
    Materials (Basel), 2022 Dec 20;16(1).
    PMID: 36614356 DOI: 10.3390/ma16010018
    Peat is a renowned problematic soil and needs stabilization to enhance its engineering properties. Silica fume (SF) and Ordinary Portland Cement (OPC) were extensively adopted to increase the mechanical properties of peat; however, their microstructural analysis is lacking. Investigated herein is the microstructural evolution caused by the OPC and SF implementation in peat soil stabilization. Initially, the compositional analysis (elements and oxides) of peat and binders was carried out via energy-dispersive X-ray (EDX) and X-ray fluorescence (XRF). Subsequently, the microstructural changes that occurred in the stabilized peat were examined through a series of microstructural analyses. The analysis includes scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FTIR), and thermogravimetric analysis (TGA) for morphological, mineralogical, functional group analysis, and bond thermal analysis, respectively. The SEM micrographs evidence the transformation of loosely packed with large micropores of untreated peat into a compact dense peat matrix. This transformation is due to the formation of newly developed minerals, i.e., calcium hydrates (CH), calcium silicate hydrates (C-S-H), calcium aluminate hydrate (CAH), ettringite (Aft) caused by the pozzolanic reaction of binders as recorded by the XRD. Similarly, different molecular functional groups were found in the FTIR analysis with the incorporation of SF and OPC. Finally, the percentage of mass loss was assessed through TGA analysis revealing the decomposition of stabilized in the second and third stages.
  20. Ting YW, Jalaludin MY, Zaini AA, Mohamed R
    Front Endocrinol (Lausanne), 2022;13:1071350.
    PMID: 36589844 DOI: 10.3389/fendo.2022.1071350
    BACKGROUND: Insulin resistance (IR), one of the key components of the metabolic syndrome, is recognized as the pathophysiological hallmark of non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the relationship between surrogate markers of IR and the severity of NAFLD among overweight or obese children.

    METHODOLOGY: A total of 56 consecutive children aged 6 to 18 years old were recruited from the pediatric obesity and type 2 diabetes mellitus (T2DM) clinic in University Malaya Medical Centre (UMMC) from 2016 to 2019. Data on anthropometric measurements, clinical components of metabolic syndrome and fasting serum insulin were collected. Triglyceride to high-density lipoprotein cholesterol ratio (TG: HDL-C), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) and Single Point Insulin Sensitivity Estimator (SPISE) were calculated. Transient elastography was performed with hepatic steatosis and liver fibrosis assessed by controlled attenuation parameter (CAP) and liver stiffness measurement (LSM), respectively.

    RESULTS: A total of 44 children (78.6%) had liver steatosis and 35.7% had presence of significant liver fibrosis (stage F≥2). Majority (89.3%) are obese and 24 children (42.9%) were diagnosed with metabolic syndrome. Higher number of children with T2DM and significant liver fibrosis were associated with higher tertiles of TG: HDL-C ratio (p<0.05). Top tertile of TG: HDL-C ratio was an independent predictor of liver fibrosis (OR=8.14, 95%CI: 1.24-53.36, p=0.029). ROC analysis showed that the area under the curve (AUC) of HOMA-IR (0.77) and TG: HDL-C ratio (0.71) were greater than that of metabolic syndrome (0.70), T2DM (0.62) and SPISE (0.22). The optimal cut-off values of HOMA-IR and TG: HDL-C ratio for detecting liver fibrosis among children with NAFLD are 5.20 and 1.58, respectively.

    CONCLUSION: Children with NAFLD and higher TG: HDL-C ratio are more likely to have liver fibrosis. TG: HDL-C ratio is a promising tool to risk stratify those with NAFLD who are at risk of developing advanced liver disease.

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