MyMedR

Displaying publications 21 - 40 of 167 in total

Abstract:

Sort:

Survey of microhymenoptera (Hymenoptera: Chalcidoidea) parasitizing filth flies (Diptera: Muscidae, Calliphoridae) breeding in refuse and poultry farms in peninsular Malaysia

Sulaiman S, Omar B, Omar S, Jeffery J, Ghauth I, Busparani V

J Med Entomol, 1990 Sep;27(5):851-5.
PMID: 2231622

Nine species of parasitoids were found parasitizing the pupae of filth flies breeding in refuse dumps and poultry farms throughout peninsular Malaysia. Spalangia were most common, consisting of Spalangia endius Walker, S. cameroni Perkins, S. gemina Boucek, S. nigroaenea Curtis, and two undescribed species. Other parasitoids collected were Pachycrepoideus vindemmiae Rondani, Dirhinus himalayanus Westwood, and an unidentified Hymenoptera. The parasitized fly hosts included Musca domestica L., Chrysomya megacephala (F.), Fannia sp., and Ophyra sp. S. endius was the most common parasitoid attacking M. domestica and C. megacephala at refuse dumps and poultry farms D. himalayanus were found to parasitize only M. domestica pupae collected at poultry farms.
Seasonal population patterns of Spalangia endius Walker (Hymenoptera: Chalcidoidea) at a refuse dumping ground in Malaysia

Sulaiman S, Omar B, Omar S, Jeffery J, Ghauth I, Busparani V

J Med Entomol, 1991 Nov;28(6):757-9.
PMID: 1770508

A study on population patterns of the parasitoid Spalangia endius Walker at a dumping ground near Kuala Lumpur city showed that the percentage of S. endius adult emergence varied seasonally. During the relatively heavy rainfall months of August and November 1988, and January, March, and April 1989, the population of S. endius adult emergence were low (0-14.2%) compared to the less rainy months of July, September, and December 1988, and May 1989 (29.3-39.6%). This information could be useful in formulating strategies to reduce house fly population at the refuse dumping ground through integrated pest management programs.
Fulltext Screening of antenatal mothers and prevention of perinatal group B streptococcal infection

Raj, M., Razali N,, Sulaiman, S.

JUMMEC, 2009;12(1):27-30.
MyJurnal

The objectives of this study were: (1) to detect group B streptococcus (GBS) carriers among antenatal patients during pregnancy ( 35 to 37 weeks) in the University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia, and (2) to give antibiotic prophylaxis during the intrapartum period for the GBS carrier patients. The aim is to prevent early onset of GBS infection in newborn. This pilot study was carried out from 1st July 2005 to 31st August 2005. It is a prospective study involving 56 pregnant women who were seen at the antenatal clinic in the UMMC between 35 to 37 weeks gestation. High vaginal swab and low vaginal together with endoanal swab were taken for culture and sensitivity. The antibiotic prophylaxis was started early in the intrapartum period for patients with positive culture for GBS. GBS was detected in 18 patients which contributes to about 32% of the study population. The proper choice of antibiotic is important in successful disease prevention.

Study site: Obstretic and gynaecological clinic, University Malaya Medical Centre (UMMC)
Screening antimicrobial activity of various extracts of Urtica dioica

Modarresi-Chahardehi A, Ibrahim D, Fariza-Sulaiman S, Mousavi L

Rev. Biol. Trop., 2012 Dec;60(4):1567-76.
PMID: 23342511

Urtica dioica or stinging nettle is traditionally used as an herbal medicine in Western Asia. The current study represents the investigation of antimicrobial activity of U. dioica from nine crude extracts that were prepared using different organic solvents, obtained from two extraction methods: the Soxhlet extractor (Method I), which included the use of four solvents with ethyl acetate and hexane, or the sequential partitions (Method II) with a five solvent system (butanol). The antibacterial and antifungal activities of crude extracts were tested against 28 bacteria, three yeast strains and seven fungal isolates by the disc diffusion and broth dilution methods. Amoxicillin was used as positive control for bacteria strains, vancomycin for Streptococcus sp., miconazole nitrate (30 microg/mL) as positive control for fungi and yeast, and pure methanol (v/v) as negative control. The disc diffusion assay was used to determine the sensitivity of the samples, whilst the broth dilution method was used for the determination of the minimal inhibition concentration (MIC). The ethyl acetate and hexane extract from extraction method I (EA I and HE I) exhibited highest inhibition against some pathogenic bacteria such as Bacillus cereus, MRSA and Vibrio parahaemolyticus. A selection of extracts that showed some activity was further tested for the MIC and minimal bactericidal concentrations (MBC). MIC values of Bacillus subtilis and Methicillin-resistant Staphylococcus aureus (MRSA) using butanol extract of extraction method II (BE II) were 8.33 and 16.33mg/mL, respectively; while the MIC value using ethyl acetate extract of extraction method II (EAE II) for Vibrio parahaemolyticus was 0.13mg/mL. Our study showed that 47.06% of extracts inhibited Gram-negative (8 out of 17), and 63.63% of extracts also inhibited Gram-positive bacteria (7 out of 11); besides, statistically the frequency of antimicrobial activity was 13.45% (35 out of 342) which in this among 21.71% belongs to antimicrobial activity extracts from extraction method I (33 out of 152 of crude extracts) and 6.82% from extraction method II (13 out of 190 of crude extracts). However, crude extracts from method I exhibited better antimicrobial activity against the Gram-positive bacteria than the Gram-negative bacteria. The positive results on medicinal plants screening for antibacterial activity constitutes primary information for further phytochemical and pharmacological studies. Therefore, the extracts could be suitable as antimicrobial agents in pharmaceutical and food industry.
Fulltext Risk perception and knowledge regarding diabetes among offspring of type 2 diabetics at a tertiary institution

Sulaiman S, Leelavathi M, Norasyikin AW

Med J Malaysia, 2020 11;75(6):655-659.
PMID: 33219173

INTRODUCTION: The rapid increase in Type 2 Diabetes Mellitus (T2DM) among the younger age groups is a growing concern worldwide. Thus, assessing the perception of risk and knowledge among those at risk may provide an opportunity for early intervention, delay or even prevent the onset.
OBJECTIVE: The aim of this study was to determine the perceived risk of developing T2DM and its knowledge among the offspring of diabetic patients.
METHODS: A cross sectional study using a self-administered questionnaire was conducted among university students whose parents had type 2 diabetes to determine their perceived risk and knowledge on T2DM.
RESULTS: A total of 336 university students participated in this study and 56.5% of them correctly identified themselves at risk of developing T2DM. About half of them (52.7%) had higher knowledge of T2DM. Male students appeared to have better risk perception (p=0.024) compared to females while those with mothers affected by T2DM appear to have higher knowledge regarding diabetes (p=0.007). Most of their information regarding T2DM was obtained from the internet (87.5%) and other family members (77.9%).
CONCLUSION: The students whose mothers had T2DM appear to have better knowledge and information regarding T2DM and this was mostly obtained from the internet and family members. Awareness regarding diabetes and healthy lifestyles advice through primary patients may be beneficial for their offspring.
Study site: Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Fulltext Review on grain size effects on thermal conductivity in ZnO thermoelectric materials

Sulaiman S, Izman S, Uday MB, Omar MF

RSC Adv, 2022 Feb 10;12(9):5428-5438.
PMID: 35425550 DOI: 10.1039/d1ra06133j

Thermoelectric materials have recently attracted a lot of attention due to their ability to convert waste heat into electricity. Based on the extensive research in this area, the nanostructuring approach has been viewed as an effective strategy for increasing thermoelectric performance. This approach focuses on the formation and growth of the superfine, pure and uniform grain size. Since the grain size has a strong influence on the thermal conductivity, this can be reduced by increasing the phonon scattering at grain boundaries and refining the grain sizes. Therefore, this review aims to discuss the mechanism of reduction in thermal conductivity in small-grain zinc oxide (ZnO) and the optimization techniques for obtaining ZnO nanoparticles with desirably low thermal conductivity and excellent thermoelectric performance.
Fulltext Resource Utilization Pattern And Cost Of Tuberculosis Treatment In Penang, Malaysia

Atif M, Sulaiman SA, Shafie AA, Asif M

Value Health, 2014 Nov;17(7):A725-6.
PMID: 27202579 DOI: 10.1016/j.jval.2014.08.051
Fulltext Residual effects of TMOF-Bti formulations against 1(st) instar Aedes aegypti Linnaeus larvae outside laboratory

Saiful AN, Lau MS, Sulaiman S, Hidayatulfathi O

Asian Pac J Trop Biomed, 2012 Apr;2(4):315-9.
PMID: 23569922 DOI: 10.1016/S2221-1691(12)60031-8

To evaluate the effectiveness and residual effects of trypsin modulating oostatic factor-Bacillus thuringiensis israeliensis (TMOF-Bti) formulations against Aedes aegypti (Ae. aegypti) (L.) larvae at UKM Campus Kuala Lumpur.
Repellency of essential oil of Piper aduncum against Aedes albopictus in the laboratory

Misni N, Sulaiman S, Othman H, Omar B

J Am Mosq Control Assoc, 2009 Dec;25(4):442-7.
PMID: 20099591

The repellent activity of Piper aduncum essential oil against Aedes albopictus was investigated under laboratory conditions with human volunteers. The lowest median effective dose (ED50) value was 1.5 microg/cm2 at 60 sec of exposure when compared to 90 sec (2.1 microg/cm2) and 120 sec (1.8 microg/cm2) of exposure. At 0.4 g, the essential oil gave a high protection (95.2%) against Ae. albopictus bites or landing at 2 h postapplication. The percentage of protection was reduced to 83.3% after 4 h, 64.5% after 6 h, and 51.6% after 8 h postapplication. As a comparison, treatment with 10% deet gave 100% protection against mosquito biting/landing for 4 h postapplication. There was no significant difference in percentage protection reduction between the plant extract and the commercial product deet, respectively (P = 0.739). The essential oil, which was not as good as deet, still gave moderate protection against Ae. albopictus biting even until 4 h postapplication. In conclusion, the P. aduncum essential oil has the potential to be used as a repellent against the dengue/dengue hemorrhagic fever vector, Ae. albopictus.
Fulltext Relationship of objectively measured physical activity and sedentary behaviour with health-related quality of life among breast cancer survivors

Nurnazahiah A, Shahril MR, Nor Syamimi Z, Ahmad A, Sulaiman S, Lua PL

Health Qual Life Outcomes, 2020 Jul 10;18(1):222.
PMID: 32650777 DOI: 10.1186/s12955-020-01478-x

BACKGROUND: Lack of physical activity throughout one's lifetime has been associated with obesity and it is also an important risk factor of breast cancer. This study aimed to determine the relationship between objectively measured physical activity and sedentary behaviour with health-related quality of life (HRQoL) among breast cancer survivors in the East Coast region of Peninsular Malaysia.
METHODS: A cross-sectional study involving 83 breast cancer survivors was carried out in two main government referral hospitals in the region. Participants wore the ActivPAL3™ microdevice physical activity monitor for seven consecutive days. The validated European Organization for Research and Treatment of Cancer Quality of Life Questionnaires (EORTC QLQ-C30) and Breast Cancer Supplementary Measure (EORTC QLQ-BR23) were used to measure their HRQoL. Multiple linear regression analysis was conducted to determine the relationship between objectively measured physical activity and sedentary behaviour with HRQoL.
RESULTS: Longer time spent on moderate to vigorous physical activity (MVPA) was significantly associated with an improvement of HRQoL (p = 0.039) whereas longer time spent on sedentary behaviour significantly reduced the functioning score (p = 0.005). In addition, prolonged sedentary bouts were also significantly associated with better body image that led to improved HRQoL (p = 0.013).
CONCLUSIONS: The study findings suggest that an increase in the time spent on MVPA was associated with improved HRQoL while sedentary behaviour was associated with poorer HRQoL among breast cancer survivors. Thus, it is essential to displace sedentary behaviour with MVPA to improve the quality of life of breast cancer survivors.
Relationship between Breteau and House indices and cases of dengue/dengue hemorrhagic fever in Kuala Lumpur, Malaysia

Sulaiman S, Pawanchee ZA, Arifin Z, Wahab A

J Am Mosq Control Assoc, 1996 Sep;12(3 Pt 1):494-6.
PMID: 8887232

The relationship between the Breteau index, the House index, and the occurrence of dengue/dengue hemorrhagic fever in the 6 zones of Kuala Lumpur was studied throughout 1994. Cases of dengue/dengue hemorrhagic fever varied between zones and between months, ranging from 0 to 21 cases. In most of the zones in Kuala Lumpur, the occurrence of dengue/dengue hemorrhagic fever has no relationship with the Breteau and House indices. Cases of dengue/dengue hemorrhagic fever occurred in all zones despite the low Breteau and House indices.
Quantitative determination of propranolol by ultraviolet HPLC in human plasma

Salman SA, Sulaiman SA, Ismail Z, Gan SH

Toxicol. Mech. Methods, 2010 Mar;20(3):137-42.
PMID: 20128736 DOI: 10.3109/15376511003602112

Many previous published methods for the quantitative determination of propranolol (PRN) in human plasma have poor recoveries and were not validated according to the FDA guideline. The aim of this study is to develop a simple HPLC method for detecting PRN in human plasma and to validate it so that it can be applied to a clinical study. Chromatographic separation was achieved using a mixture of a mobile phase consisting of 160 ml water, 180 ml methanol, 70 ml acetonitrile, 2.5 ml acetic acid, and 125 microl triethylamine (v/v). The pH of the whole mixture was adjusted to 3.4. A flow rate of 0.5 ml/min was employed throughout with a 15 microl injection volume. Detection was done using a UV detector at 291 nm. The validated method was linear for concentrations ranging from 15-180 ng/ ml with a good separation and specificity for both PRN and its internal standard, oxprenolol (OXP), with excellent recoveries, precision, and accuracies. The limit of detection (LOD) and limit of quantification (LOQ) were 1 and 10 ng/ml, respectively. The stability studies demonstrated that PRN is stable in the autosampler vials and also up to 3.5 months. To the authors' knowledge, the recovery, that ranged between 97.9-102.7%, is the highest among all previously reported methods that used HPLC with UV detection. The developed and validated method for PRN analysis is excellent and applicable to a clinical study.
Quantifying the ultrastructure changes of air-dried and irradiated human amniotic membrane using atomic force microscopy: a preliminary study

Mohd S, Ghazali MI, Yusof N, Sulaiman S, Ramalingam S, Kamarul T, et al.

Cell Tissue Bank, 2018 Dec;19(4):613-622.
PMID: 30056604 DOI: 10.1007/s10561-018-9711-4

Air-dried and sterilized amnion has been widely used as a dressing to treat burn and partial thickness wounds. Sterilisation at the standard dose of 25 kGy was reported to cause changes in the morphological structure as observed under the scanning electron microscope. This study aimed to quantify the changes in the ultrastructure of the air-dried amnion after gamma-irradiated at several doses by using atomic force microscope. Human placentae were retrieved from mothers who had undergone cesarean elective surgery. Amnion separated from chorion was processed and air-dried for 16 h. It was cut into 10 × 10 mm, individually packed and exposed to gamma irradiation at 5, 15, 25 and 35 kGy. Changes in the ultrastructural images of the amnion were quantified in term of diameter of the epithelial cells, size of the intercellular gap and membrane surface roughness. The longest diameter of the amnion cells reduced significantly after radiation (p
Fulltext Pyrethroid Resistance in Malaysian Populations of Dengue Vector Aedes aegypti Is Mediated by CYP9 Family of Cytochrome P450 Genes

Ishak IH, Kamgang B, Ibrahim SS, Riveron JM, Irving H, Wondji CS

PLoS Negl Trop Dis, 2017 01;11(1):e0005302.
PMID: 28114328 DOI: 10.1371/journal.pntd.0005302

BACKGROUND: Dengue control and prevention rely heavily on insecticide-based interventions. However, insecticide resistance in the dengue vector Aedes aegypti, threatens the continued effectiveness of these tools. The molecular basis of the resistance remains uncharacterised in many endemic countries including Malaysia, preventing the design of evidence-based resistance management. Here, we investigated the underlying molecular basis of multiple insecticide resistance in Ae. aegypti populations across Malaysia detecting the major genes driving the metabolic resistance.
METHODOLOGY/PRINCIPAL FINDINGS: Genome-wide microarray-based transcription analysis was carried out to detect the genes associated with metabolic resistance in these populations. Comparisons of the susceptible New Orleans strain to three non-exposed multiple insecticide resistant field strains; Penang, Kuala Lumpur and Kota Bharu detected 2605, 1480 and 425 differentially expressed transcripts respectively (fold-change>2 and p-value ≤ 0.05). 204 genes were commonly over-expressed with monooxygenase P450 genes (CYP9J27, CYP6CB1, CYP9J26 and CYP9M4) consistently the most up-regulated detoxification genes in all populations, indicating that they possibly play an important role in the resistance. In addition, glutathione S-transferases, carboxylesterases and other gene families commonly associated with insecticide resistance were also over-expressed. Gene Ontology (GO) enrichment analysis indicated an over-representation of GO terms linked to resistance such as monooxygenases, carboxylesterases, glutathione S-transferases and heme-binding. Polymorphism analysis of CYP9J27 sequences revealed a high level of polymorphism (except in Joho Bharu), suggesting a limited directional selection on this gene. In silico analysis of CYP9J27 activity through modelling and docking simulations suggested that this gene is involved in the multiple resistance in Malaysian populations as it is predicted to metabolise pyrethroids, DDT and bendiocarb.
CONCLUSION/SIGNIFICANCE: The predominant over-expression of cytochrome P450s suggests that synergist-based (PBO) control tools could be utilised to improve control of this major dengue vector across Malaysia.
Fulltext Pulmonary atelectasis from compression of the left main bronchus by an aortic aneurysm

Yap KH, Sulaiman S

Singapore Med J, 2009 Jul;50(7):e247-9.
PMID: 19644610

Pulmonary atelectasis may be caused by endobronchial lesions or by extrinsic compression of the bronchus. However, lung collapse due to compression from a thoracic aneurysm is uncommon. We report a 76-year-old hypertensive female patient who has pulmonary atelectasis due to an extrinsic compression from a descending thoracic aortic aneurysm, and discuss possible treatment options.
Psychometric properties and osteoprotective behaviors among type 2 diabetic patients: osteoporosis self-efficacy scale Malay version (OSES-M)

Abdulameer SA, Syed Sulaiman SA, Hassali MA, Subramaniam K, Sahib MN

Osteoporos Int, 2013 Mar;24(3):929-40.
PMID: 22790611 DOI: 10.1007/s00198-012-2071-1

In type 2 diabetic patients (T2DM), only 22 % have normal bone mineral density and almost three quarters of the sample population had low self-efficacy towards osteoporosis. These results reflect the need for screening and educational programs to increase the awareness of T2DM towards osteoporosis.
INTRODUCTION: Our aim was to translate and examine the psychometric properties of the Malay version of the osteoporosis self-efficacy scale (OSES-M) among T2DM and to determine the best cut-off value with optimum sensitivity and specificity. In addition, to assess factors that affects diabetic patients' osteoporosis self-efficacy.
METHODS: A standard "forward-backward" procedure was used to translate the OSES into Malay language, which was then validated with a convenience sample of 250 T2DM. The sensitivity and specificity of the OSES-M was calculated using receiver operating characteristic curve analysis. Bivariate and multivariate approaches were used to examine multiple independent variables on each dependent variable.
RESULTS: The mean score of OSES-M was 731.74 ± 197.15. Fleiss' kappa, content validity ratio range, and content validity index were 0.99, 0.75-1, and 0.96, respectively. Two factors were extracted from exploratory factor analysis and were confirmed through confirmatory factor analysis. Internal consistency and test-retest reliability were 0.92 and 0.86, respectively. The optimum cut-off point of OSES-M to predict osteoporosis/osteopenia was 858. Regression analysis revealed that knowledge, health belief, and some demographic data had an impact on OSES-M.
CONCLUSIONS: The results show that the OSES-M is a reliable and valid instrument for measuring osteoporosis self-efficacy in the Malaysian clinical setting.
Fulltext Product structure initialization, the bottleneck of ERP implementation in customer driven environments: a case study

Niestanak, S. Abedi, Faieza, A.A., Sulaiman, S., Rosnah, M.Y.

Pertanika Journal of Science & Technology, 2011;19(1):77-92.
MyJurnal

This study of the implementation of enterprise resource planning (ERP) in a customer driven environment analyzes the critical success factors throughout the initialization phase. The dynamic and stochastic nature of customer driven environments results in a massive workload of product structure configuration tasks related to new arrivals on one hand and a constant updating process on the other. Meanwhile, the development and implementation of an ERP system was studied from the very first step (i.e. the feasibly study for implementing an ERP) to the last step (i.e. testing the outputs of the implemented system) in an office furniture company for three years. The study involved analyzing of the data collected that were from a series of interviews, as well as direct observations and reviewing of the company’s documents. Based on the output of the analysis phase, a top-down hierarchical analysis of goals and CSFs were carried out according to the CSF analysis method. Three top level objectives included reducing project failure risk, project cost, and project time. Analysing the primary results of the study (i.e. activity model, data flow diagram DFD of different levels, system problems and potential solutions descriptions, etc.) revealed that the critical phase of the implementation project would be product structure initialization and this should be taken into consideration as the bottleneck of production planning in customer driven environment, which dramatically reduced the ERP efficiency in this kind of environment. Moreover, initializing issues of the same process is the main obstacle to the success of the ERP implementation, as it considerably raises the project failure risk and cost. Therefore, the simplification, facilitation, and automation of the PSCM process, which lead to acceleration of this process, are the most significant success factors for the ERP implementation projects in customer driven environment.
Fulltext Prevalence of plasmid-bearing and plasmid-free Chlamydia trachomatis infection among women who visited obstetrics and gynecology clinics in Malaysia

Yeow TC, Wong WF, Sabet NS, Sulaiman S, Shahhosseini F, Tan GM, et al.

BMC Microbiol, 2016 Mar 18;16:45.
PMID: 26987367 DOI: 10.1186/s12866-016-0671-1

BACKGROUND: The 7.5 kb cryptic plasmid of Chlamydia trachomatis has been shown to be a virulence factor in animal models, but its significance in humans still remains unknown. The aim of this study was to investigate the prevalence and potential involvement of the C. trachomatis cryptic plasmid in causing various clinical manifestations; including infertility, reproductive tract disintegrity, menstrual disorder, and polycystic ovarian syndrome (PCOS) among genital C. trachomatis-infected patients.
RESULTS:A tot l of 180 female patients of child bearing age (mean 30.9 years old, IQR:27-35) with gynecological complications and subfertility issues, who visited Obstetrics and Gynecology clinics in Kuala Lumpur, Malaysia were recruited for the study. Prevalence of genital chlamydial infection among these patients was alarmingly high at 51.1% (92/180). Of the 92 chlamydia-infected patients, 93.5% (86/92) were infected with plasmid-bearing (+) C. trachomatis while the remaining 6.5% (6/92) were caused by the plasmid-free (-) variant. Our data showed that genital C. trachomatis infection was associated with infertility issues, inflammation in the reproductive tract (mucopurulent cervicitis or endometriosis), irregular menstrual cycles and polycystic ovarian syndrome (PCOS). However, no statistical significance was detected among patients with plasmid (+) versus plasmid (-) C. trachomatis infection. Interestingly, plasmid (+) C. trachomatis was detected in all patients with PCOS, and the plasmid copy numbers were significantly higher among PCOS patients, relative to non-PCOS patients.
CONCLUSION: Our findings show a high incidence of C. trachomatis infection among women with infertility or gynecological problems in Malaysia. However, due to the low number of plasmid (-) C. trachomatis cases, a significant role of the plasmid in causing virulence in human requires further investigation of a larger cohort.
KEYWORDS: Chlamydia trachomatis; Infertility; Plasmid; Reproductive system disorders
Study site: Obstetrics and Gynecology clinic, University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia
Fulltext Prevalence of metabolic syndrome among breast cancer survivors in East Coast of Peninsular Malaysia

Shahril MR, Amirfaiz S, Lua PL, Nurnazahiah A, Zakarai NS, Kow VL, et al.

BMC Public Health, 2021 01 28;21(1):238.
PMID: 33509143 DOI: 10.1186/s12889-021-10288-9

BACKGROUND: To date, limited data are available on metabolic syndrome prevalence among breast cancer survivors in Malaysia. Therefore, this study was conducted to determine the prevalence of metabolic syndrome and abnormal metabolic syndrome components among breast cancer survivors in East Coast of Peninsular Malaysia.
METHODS: This cross-sectional study included 95 breast cancer survivors (age 53.7 ± 7.6 years) who have completed main cancer treatments for ≥6 months. Cancer survivors were recruited from two main government hospitals in Kelantan and Terengganu using a purposive sampling method.
RESULTS: According to the Harmonized criteria, the metabolic syndrome prevalence was 50.5%. Among those with metabolic syndrome, the most prevalent abnormal metabolic components were triglycerides (91.2%), fasting blood glucose (79.6%) and HDL-c level (78.4%). Except for total cholesterol and LDL-c, all other metabolic syndrome components were significantly different (p
Physical and Natural Crosslinking Approaches on Three-Dimensional Gelatin Microspheres for Cartilage Regeneration

Sulaiman S, Rani RA, Mohamad Yahaya NH, Tabata Y, Hiraoka Y, Seet WT, et al.

Tissue Eng Part C Methods, 2022 10;28(10):557-569.
PMID: 35615885 DOI: 10.1089/ten.TEC.2022.0073

The use of gelatin microspheres (GMs) as a cell carrier has been extensively researched. One of its limitations is that it dissolves rapidly in aqueous settings, precluding its use for long-term cell propagation. This circumstance necessitates the use of crosslinking agents to circumvent the constraint. Thus, this study examines two different methods of crosslinking and their effect on the microsphere's physicochemical and cartilage tissue regeneration capacity. Crosslinking was accomplished by physical (dehydrothermal [DHT]) and natural (genipin) crosslinking of the three-dimensional (3D) GM. We begin by comparing the microstructures of the scaffolds and their long-term resistance to degradation under physiological conditions (in an isotonic solution, at 37°C, pH = 7.4). Infrared spectroscopy indicated that the gelatin structure was preserved after the crosslinking treatments. The crosslinked GM demonstrated good cell adhesion, viability, proliferation, and widespread 3D scaffold colonization when seeded with human bone marrow mesenchymal stem cells. In addition, the crosslinked microspheres enhanced chondrogenesis, as demonstrated by the data. It was discovered that crosslinked GM increased the expression of cartilage-related genes and the biosynthesis of a glycosaminoglycan-positive matrix as compared with non-crosslinked GM. In comparison, DHT-crosslinked results were significantly enhanced. To summarize, DHT treatment was found to be a superior approach for crosslinking the GM to promote better cartilage tissue regeneration.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links