MyMedR

Displaying publications 561 - 580 of 1094 in total

Abstract:

Sort:

Reflexin A, a new indole alkaloid from Rauvolfia reflexa induces apoptosis against colon cancer cells

Fadaeinasab M, Karimian H, Omar H, Taha H, Khorasani A, Banisalam B, et al.

J Asian Nat Prod Res, 2020 May;22(5):474-488.
PMID: 30945944 DOI: 10.1080/10286020.2019.1588888

One new indole alkaloid, reflexin A (1), and two known indoles, macusine B (2) and vinorine (3), were isolated from the bark of Rauvolfia reflexa. Their structures were elucidated by 1D and 2D NMR, UV, IR, and MS spectroscopic analyses. Compound 1 displayed anticancer activity against HCT-116 colon cancer cells with an IC50 value of 30.24 ± 0.75 µM. The results implied that the newly isolated 1 induced apoptosis in HCT-116 cells, suggesting its possible role as an anticancer agent. In vivo acute toxicity study was performed on compound 1 to evaluate its safety profile.
Comparative evaluation of bioburden and sterility of indigenously prepared bone allograft with and without gentamicin

Makker K, Lamba AK, Faraz F, Tandon S, Sheikh Ab Hamid S, Aggarwal K, et al.

Cell Tissue Bank, 2019 Jun;20(2):243-253.
PMID: 30903410 DOI: 10.1007/s10561-019-09763-w

During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden and sterility in the obtained grafts to evaluate the role of antibiotic in enhancing graft safety. Fifty samples of demineralized freeze-dried bone allograft were prepared from suitable donors according to international standards. Randomly selected 25 samples were placed in 8 mg gentamicin/gram bone solution for 1 h. Packaging and sealing was done to ensure no microbial ingress during transportation. 40 samples were selected for bioburden testing. Remaining 10 were subjected to 25 kGy gamma radiation and tested for sterility. Microbiological evaluation revealed no evidence of colony forming units in all the samples of both the groups (Bioburden = 0). Post-radiation sterility testing also revealed no bacterial colony in the tested samples from both the groups. Favorable results validate the processing protocol while comparable results in both groups indicate no additive benefit of gentamicin addition. Nil bioburden may be used in further studies to determine a lower radiation dose to achieve adequate sterility and minimize the disadvantages of radiation like collagen cross-linking and decreased osteoinductive capacity.
Rosmarinic acid attenuates inflammation in experimentally induced arthritis in Wistar rats, using Freund's complete adjuvant

Gautam RK, Gupta G, Sharma S, Hatware K, Patil K, Sharma K, et al.

Int J Rheum Dis, 2019 Jul;22(7):1247-1254.
PMID: 31155849 DOI: 10.1111/1756-185X.13602

AIM: The purpose of our investigation is to evaluate the anti-arthritic potential of isolated rosmarinic acid from the rind of Punica granatum.
METHOD: Rosmarinic acid was isolated by bioactivity-guided isolation from butanolic fraction of Punica granatum and acute toxicity of rosmarinic acid was carried out. The experiment was conducted at doses of 25 and 50 mg/kg, in Freund's complete adjuvant (FCA)-induced arthritic rats. Various parameters, that is arthritic score, paw volume, thickness of paw, hematological, antioxidant and inflammatory parameters such as glutathione (GSH), superoxide dismutase (SOD), malonaldehyde (MDA) and tumor necrosis factor-α (TNF-α) were also estimated.
RESULTS: Rosmarinic acid significantly decreased the arthritic score, paw volume, joint diameter, white blood cell count and erythrocyte sedimentation rate. It also significantly increased body weight, hemoglobin and red blood cells. The significantly decreased levels of TNF-α were observed in treated groups as compared to arthritic control rats (P
Fulltext COVID-19 transmission through host cell directed network of GPCR

Singh Y, Gupta G, Satija S, Pabreja K, Chellappan DK, Dua K

Drug Dev Res, 2020 09;81(6):647-649.
PMID: 32329083 DOI: 10.1002/ddr.21674
Fulltext RAAS blockers in hypertension posing a higher risk toward the COVID-19

Singh Y, Gupta G, Satija S, Negi P, Chellappan DK, Dua K

Dermatol Ther, 2020 Jul;33(4):e13501.
PMID: 32359088 DOI: 10.1111/dth.13501
Fulltext Allelopathic Effect of Selected Rice (Oryza sativa) Varieties against Barnyard Grass (Echinochloa cruss-gulli)

Rahaman F, Juraimi AS, Rafii MY, Uddin MK, Hassan L, Chowdhury AK, et al.

Plants (Basel), 2021 Sep 26;10(10).
PMID: 34685826 DOI: 10.3390/plants10102017

Rice has been subjected to a great deal of stress during its brief existence, but it nevertheless ranked first among cereal crops in terms of demand and productivity. Weeds are characterized as one of the major biotic stresses by many researchers. This research aims to determine the most potential allelopathic rice variety among selected rice accessions. For obtaining preeminent varieties, seventeen rice genotypes were collected from Bangladesh and Malaysia. Two prevalent procedures, relay seeding and the sandwich technique were employed to screen the seventeen rice (donor) accessions against barnyard grass (tested plant). In both approaches, only the BR17 variety demonstrated substantial inhibition of germination percentage, root length, and dry matter of barnyard grass. The rice variety BR17 exclusively took the zenith position, and it inhibited the development of barnyard grass by more than 40-41% on an average. BR17 is originated from KN-1B-361-1-8-6-10 (Indonesia) and developed by the Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh in 1985, having a high yielding capacity of more than 6 t/ha. Our study suggested that the usage of the allelopathy-weed inverse relationship to treat the weed problem can be a fantastic choice in the twenty-first century.
EEG feature fusion for motor imagery: A new robust framework towards stroke patients rehabilitation

Al-Qazzaz NK, Alyasseri ZAA, Abdulkareem KH, Ali NS, Al-Mhiqani MN, Guger C

Comput Biol Med, 2021 10;137:104799.
PMID: 34478922 DOI: 10.1016/j.compbiomed.2021.104799

Stroke is the second foremost cause of death worldwide and is one of the most common causes of disability. Several approaches have been proposed to manage stroke patient rehabilitation such as robotic devices and virtual reality systems, and researchers have found that the brain-computer interfaces (BCI) approaches can provide better results. Therefore, the most challenging tasks with BCI applications involve identifying the best technique(s) that can reveal the neuron stimulus information from the patients' brains and extracting the most effective features from these signals as well. Accordingly, the main novelty of this paper is twofold: propose a new feature fusion method for motor imagery (MI)-based BCI and develop an automatic MI framework to detect the changes pre- and post-rehabilitation. This study investigated the electroencephalography (EEG) dataset from post-stroke patients with upper extremity hemiparesis. All patients performed 25 MI-based BCI sessions with follow up assessment visits to examine the functional changes before and after EEG neurorehabilitation. In the first stage, conventional filters and automatic independent component analysis with wavelet transform (AICA-WT) denoising technique were used. Next, attributes from time, entropy and frequency domains were computed, and the effective features were combined into time-entropy-frequency (TEF) attributes. Consequently, the AICA-WT and the TEF fusion set were utilised to develop an AICA-WT-TEF framework. Then, support vector machine (SVM), k-nearest neighbours (kNN) and random forest (RF) classification technique were tested for MI-based BCI rehabilitation. The proposed AICA-WT-TEF framework with RF classifier achieves the best results compared with other classifiers. Finally, the proposed framework and feature fusion set achieve a significant performance in terms of accuracy measures compared to the state-of-the-art. Therefore, the proposed methods could be crucial for improving the process of automatic MI rehabilitation and are recommended for implementation in real-time applications.
Current trends in polymerase chain reaction based detection of three major human pathogenic vibrios

Bonny SQ, Hossain MAM, Uddin SMK, Pulingam T, Sagadevan S, Johan MR

Crit Rev Food Sci Nutr, 2022;62(5):1317-1335.
PMID: 33146031 DOI: 10.1080/10408398.2020.1841728

Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus are the most significant aquatic pathogens of the genera Vibrio, account for most Vibrio-associated outbreaks worldwide. Rapid identification of these pathogens is of great importance for disease surveillance, outbreak investigations and food safety maintenance. Traditional culture dependent methods are time-consuming and labor-intensive whereas culture-independent polymerase chain reaction (PCR) based assays are reliable, consistent, rapid and reproducible. This review covers the recent development and applications of PCR based techniques, which have accelerated advances in the analysis of nucleic acids to identify three major pathogenic vibrios. Emphasis has been given to analytical approaches as well as advantages and limits of the available methods. Overall, this review article possesses the substantial merit to be used as a reference guide for the researchers to develop improved PCR based techniques for the differential detection and quantification of Vibrio species.
Fulltext Overcoming drug delivery barriers and challenges in topical therapy of atopic dermatitis: A nanotechnological perspective

Hemrajani C, Negi P, Parashar A, Gupta G, Jha NK, Singh SK, et al.

Biomed Pharmacother, 2022 Mar;147:112633.
PMID: 35030434 DOI: 10.1016/j.biopha.2022.112633

Atopic dermatitis (AD) is an inflammatory disorder centered around loss of epidermal barrier function, and T helper 2 (Th2) immune responses. The current understanding of disease heterogeneity and complexity, limits the rational use of existing topical, systemic therapeutic agents, but paves way for development of advanced therapeutic agents. Additionally, advanced nanocarriers that deliver therapeutics to target cells, seem to offer a promising strategy, to overcome intrinsic limitations and challenges of conventional, and traditional drug delivery systems. Ever-evolving understanding of molecular target sites and complex pathophysiology, adverse effects of current therapeutic options, inefficient disease recapitulation by existing animal models are some of the challenges that we face. Also, despite limited success in market translatibility, nanocarriers have demonstrated excellent preclinical results and have been extensively studied for AD. Detailed research on behavior of nanocarriers in different patients and tailored therapy to account for phenotypic variability of the disease are the new research avenues that we look forward to.
Metformin: A Salutary Candidate for Colorectal Cancer Treatment in Patients with Diabetes

Samuel VP, Dahiya R, Singh Y, Gupta G, Sah SK, Gubbiyappa SK, et al.

J Environ Pathol Toxicol Oncol, 2019;38(2):133-141.
PMID: 31679276 DOI: 10.1615/JEnvironPatholToxicolOncol.2019029388

The current study is a review of the literature on patients with diabetes who are diagnosed with colorectal cancer (CRC), encompassing recent research on CRC and the molecular level changes occurring in these patients on the basis of varying environmental as well as non-environmental factors. It has been noted that nearly 50% of all patients undergo the systemic treatment module; however, most of them exhibit drug resistance. In addition, targeted gene therapy has also been used in treatment but has been found to be effective only in patients with a specified molecular profile (or else this might lead to an increased risk of developing resistant mutations). This has led to increasing interest among researchers in finding innovative treatment options. Metformin, a biguanide, has been widely used in treating diabetes. The drug has been reportedly used in cases of hypothesis-generating retrospective population studies of diabetic patients showing reduced incidence of cancer. Metformin helps in reduction of excess insulin levels that possess various effects on cell signaling and metabolism. Nonetheless, there is need for an in-depth study on its molecular mechanism to fill any existing research gaps.
Protective effect of pioglitazone, a PPARγ agonist against acetaminophen-induced hepatotoxicity in rats

Gupta G, Krishna G, Chellappan DK, Gubbiyappa KS, Candasamy M, Dua K

Mol Cell Biochem, 2014 Aug;393(1-2):223-8.
PMID: 24771068 DOI: 10.1007/s11010-014-2064-9

Acetaminophen has a reasonable safety profile when consumed in therapeutic doses. However, it could induce hepatotoxicity and even acute liver failure when taken at an overdose. Pioglitazone, PPARγ ligand, is clinically tested and used in treatment of diabetes. PPARγ is a key nuclear hormone receptor of lipid metabolisms and regulates several gene transcriptions associated with differentiation, growth arrest, and apoptosis. The aim of our study was to evaluate the hepatoprotective activity of pioglitazone on acetaminophen-induced hepatotoxicity and to understand the relationship between the PPARγ and acetaminophen-induced hepato injury. For the experiment, Sprague-Dawley rats (160-180 g) were used and divided into four groups. Groups I and II were normal and experimental controls, respectively. Groups III and IV received the pioglitazone 20 mg/kg for 10 days. Hepatotoxicity was induced in Groups II and III on the eighth day with acetaminophen (i.p. 350 mg/kg body weight). The hepatoprotective effect was evaluated by performing an assay of the total protein, total bilirubin, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and α-fetoprotein as well as glutathione peroxidase, lipid peroxidation, catalase, superoxide dismutase, and glutathione transferase and liver histopathology. The assay results were presented as mean and standard error of mean for each group. The study group was compared with the control group by one-way ANOVA test. A p value of <0.05 was considered significant. Pioglitazone significantly reduced the elevated level of above serum marker enzymes and also inhibits the free radical formation by scavenging hydroxyl ions. It also restored the level of LPO and significantly elevated the levels of endogenous antioxidant enzymes in acetaminophen-challenged hepatotoxicity. Liver histopathological examination showed that pioglitazone administration antagonized acetaminophen -induced liver pathological damage. Various biochemical estimations of different hepatic markers and antioxidant enzymes and histopathological studies of liver tissues glimpse a support to its significant hepatoprotective activity on acetaminophen -induced hepatotoxicity.
Inclusion of gerodontology in dental curriculum: An urgent case for India

Shigli K, Nayak SS, Lagali-Jirge V, Kusurkar RA, Nerali JT, Oginni FO

Gerontol Geriatr Educ, 2022 04 15;43(4):468-481.
PMID: 35422199 DOI: 10.1080/02701960.2022.2058936

The proportion of older adults is on the rise. Management of dental problems in this group is different from the general population, and hence requires special training. Gerodontology is yet to find its place in the Indian dental curriculum. A lack of training would result in inadequate care delivery. In this article, we share our views on the need for inclusion of the subject, potential challenges, and a guide for incorporation of gerodontology in undergraduate and postgraduate curriculum in the Indian dental institutes. We propose a framework based on the salient features of Kern's 6-step approach for curriculum development and Kotter's 8-step change management model. Some features are common to both the models. A combination of these models includes the following salient features: Problem identification and general needs assessment, beginning with a sense of urgency and targeted needs assessment, communication of the vision for change, working in guided coalitions and defining clear goals and objectives, adopting the relevant educational strategies, implementation strategies to enable change and generating short-term wins, evaluating the effectiveness of the curricular reform and sustaining and anchoring the change. The proposed framework may also be useful for countries where gerodontology is yet to be implemented.
Berberine loaded liquid crystalline nanostructure inhibits cancer progression in adenocarcinomic human alveolar basal epithelial cells in vitro

Mehta M, Malyla V, Paudel KR, Chellappan DK, Hansbro PM, Oliver BG, et al.

J Food Biochem, 2021 11;45(11):e13954.
PMID: 34609010 DOI: 10.1111/jfbc.13954

Metastasis represents the leading cause of death in lung cancer patients. C-X-C Motif Chemokine Ligand 8 (CXCL-8), Chemokine (C-C motif) ligand 20 (CCL-20) and heme oxygenase -1 (HO-1) play an important role in cancer cell proliferation and migration. Berberine is an isoquinoline alkaloid isolated from several herbs in the Papaveraceae family that exhibits anti-inflammatory, anticancer and antidiabetic properties. Therefore, the aim of present study is to investigate the inhibitory potential of berberine monoolein loaded liquid crystalline nanoparticles (berberine-LCNs) against cancer progression. Berberine-LCNs were prepared by mixing berberine, monoolein and poloxamer 407 (P407) using ultrasonication method. A549 cells were treated with or without 5 µM dose of berberine LCNs for 24 hr and total cellular protein was extracted and further analyzed for the protein expression of CCl-20, CXCL-8 and HO-1 using human oncology array kit. Our results showed that berberine-LCNs significantly reduced the expression of CCl-20, CXCL-8 and HO-1 at dose of 5µM. Collectively, our findings suggest that berberine-LCNs have inhibitory effect on inflammation/oxidative stress related cytokines i.e. CCL20, CXCL-8, and HO-1 which could be a novel therapeutic target for the management of lung cancer. PRACTICAL APPLICATIONS: Berberine is an isoquinoline alkaloid extracted from various plants of Papaveraceae family. CXCL-8, CCL-20 and HO-1 play an important role in cancer progression. Our study showed that Berberine LCNs significantly downregulate the expression of CXCL-8, CCL-20 and HO-1 which suggests that Berberine loaded nanoparticles could be a promising therapeutic alternative for the management of lung cancer.
Fulltext Circadian Rhythm Disruption and Alzheimer's Disease: The Dynamics of a Vicious Cycle

Sharma A, Sethi G, Tambuwala MM, Aljabali AAA, Chellappan DK, Dua K, et al.

Curr Neuropharmacol, 2021;19(2):248-264.
PMID: 32348224 DOI: 10.2174/1570159X18666200429013041

All mammalian cells exhibit circadian rhythm in cellular metabolism and energetics. Autonomous cellular clocks are modulated by various pathways that are essential for robust time keeping. In addition to the canonical transcriptional translational feedback loop, several new pathways of circadian timekeeping - non-transcriptional oscillations, post-translational modifications, epigenetics and cellular signaling in the circadian clock - have been identified. The physiology of circadian rhythm is expansive, and its link to the neurodegeneration is multifactorial. Circadian rhythm disruption is prevelant in contamporary society where light-noise, shift-work, and transmeridian travel are commonplace, and is also reported from the early stages of Alzheimer's disease (AD). Circadian alignment by bright light therapy in conjunction with chronobiotics is beneficial for treating sundowning syndrome and other cognitive symptoms in advanced AD patients. We performed a comprehensive analysis of the clinical and translational reports to review the physiology of the circadian clock, delineate its dysfunction in AD, and unravel the dynamics of the vicious cycle between two pathologies. The review delineates the role of putative targets like clock proteins PER, CLOCK, BMAL1, ROR, and clock-controlled proteins like AVP, SIRT1, FOXO, and PK2 towards future approaches for management of AD. Furthermore, the role of circadian rhythm disruption in aging is delineated.
Bacterial biofilms associated skin disorders: Pathogenesis, advanced pharmacotherapy and nanotechnology-based drug delivery systems as a treatment approach

Vyas T, Rapalli VK, Chellappan DK, Dua K, Dubey SK, Singhvi G

Life Sci, 2021 Dec 15;287:120148.
PMID: 34785190 DOI: 10.1016/j.lfs.2021.120148

BACKGROUND: Biofilms are microcolonies of microbes that form communities with a variety of microbes, exhibit the same gene composition but differ in gene expression. Biofilm-associated infections have been in existence for a long, however, biofilm-associated skin disorders have not been investigated much.
OBJECTIVES: Biofilms, which are made mostly of the matrix can be thought of as communities of microbes that are more virulent and more difficult to eradicate as compared to their planktonic counterparts. Currently, several formulations are available in the market which have the potential to treat biofilm-assisted skin disorders. However, the existing pharmacotherapies are not competent enough to cure them effectively and entirely, in several cases.
KEY FINDINGS: Especially with the rising resistance towards antibiotics, it has become particularly challenging to ameliorate these disorders completely. The new approaches are being used to combat biofilm-associated skin disorders, some of them being photodynamic therapy, nanotherapies, and the use of novel drug delivery systems. The focus of attention, however, is nanotherapy. Micelles, solid lipid nanoparticles, quatsomes, and many others are being considered to find a better solution for the biofilm-associated skin disorders.
SIGNIFICANCE: This review is an attempt to give a perspective on these new approaches for treating bacterial biofilms associated with skin disorders.
Can dextran-based nanoparticles mitigate inflammatory lung diseases?

Prasher P, Sharma M, R Wich P, Jha NK, Singh SK, Chellappan DK, et al.

Future Med Chem, 2021 12;13(23):2027-2031.
PMID: 34596425 DOI: 10.4155/fmc-2021-0218
Authentication of Halal and Kosher meat and meat products: Analytical approaches, current progresses and future prospects

Hossain MAM, Uddin SMK, Sultana S, Wahab YA, Sagadevan S, Johan MR, et al.

Crit Rev Food Sci Nutr, 2022;62(2):285-310.
PMID: 32914638 DOI: 10.1080/10408398.2020.1814691

Meat and meat products are widely consumed worldwide as a source of high-quality proteins, essential amino acids, vitamins, and necessary minerals. The acceptability of Halal and Kosher meat products relies not only on the species origin but also on the manner of slaughtering of animals. Both Islam and Judaism have their own dietary laws in their holy books regarding acceptance and forbiddance of dietary items particularly meat and meat products. They also include many strictures to follow for ritual cleanliness of foods. Since the authenticity of Halal and Kosher food created increased concerns among consumers, the integrity of Halal and Kosher meat and meat products must be assured so that consumers can be accomplished with the originality of products. There is an increasing demand for reliable and sensitive techniques for the authentication of various Halal and Kosher meat products. This up-to-date review intends to provide an updated and extensive overview critically on the present situation, progress, and challenges of analytical techniques to authenticate animal species in meat items. It also addresses slaughtering procedure with brief discussion on Halal and Kosher laws with a view to creating consumer awareness against fraudulent practices. The available methods are schematically presented, and their salient features are comparatively elucidated in tables. Potential future technologies are predicted, and probable challenges are summarized. Overall, the present review article possesses substantial merits to be served as a reference guide in the field of academia and industry for the preparation/processing and identification of Halal and Kosher meat and meat products as well as may act as a platform to help improve existing authentication methods.
Fulltext A novel state space reduction algorithm for team formation in social networks

Rehman MZ, Zamli KZ, Almutairi M, Chiroma H, Aamir M, Kader MA, et al.

PLoS One, 2021;16(12):e0259786.
PMID: 34855771 DOI: 10.1371/journal.pone.0259786

Team formation (TF) in social networks exploits graphs (i.e., vertices = experts and edges = skills) to represent a possible collaboration between the experts. These networks lead us towards building cost-effective research teams irrespective of the geolocation of the experts and the size of the dataset. Previously, large datasets were not closely inspected for the large-scale distributions & relationships among the researchers, resulting in the algorithms failing to scale well on the data. Therefore, this paper presents a novel TF algorithm for expert team formation called SSR-TF based on two metrics; communication cost and graph reduction, that will become a basis for future TF's. In SSR-TF, communication cost finds the possibility of collaboration between researchers. The graph reduction scales the large data to only appropriate skills and the experts, resulting in real-time extraction of experts for collaboration. This approach is tested on five organic and benchmark datasets, i.e., UMP, DBLP, ACM, IMDB, and Bibsonomy. The SSR-TF algorithm is able to build cost-effective teams with the most appropriate experts-resulting in the formation of more communicative teams with high expertise levels.
Fulltext Optically Transparent Tri-Wideband Mosaic Frequency Selective Surface with Low Cross-Polarisation

Mohamed Nafis NB, Himdi M, A Rahim MK, Ayop O, Dewan R

Materials (Basel), 2022 Jan 14;15(2).
PMID: 35057336 DOI: 10.3390/ma15020622

Acquiring an optically transparent feature on the wideband frequency selective surface (FSS), particularly for smart city applications (building window and transportation services) and vehicle windows, is a challenging task. Hence, this study assessed the performance of optically transparent mosaic frequency selective surfaces (MFSS) with a conductive metallic element unit cell that integrated Koch fractal and double hexagonal loop fabricated on a polycarbonate substrate. The opaque and transparent features of the MFSS were studied. While the study on opaque MFSS revealed the advantage of having wideband responses, the study on transparent MFSS was performed to determine the optical transparency application with wideband feature. To comprehend the MFSS design, the evolutionary influence of the unit cell on the performance of MFSS was investigated and discussed thoroughly in this paper. Both the opaque and transparent MFSS yielded wideband bandstop and bandpass responses with low cross-polarisation (-37 dB), whereas the angular stability was limited to only 25°. The transparent MFSS displayed high-level transparency exceeding 70%. Both the simulated and measured performance comparison exhibited good correlation for both opaque and transparent MFSS. The proposed transparent MFSS with wideband frequency response and low cross-polarisation features signified a promising filtering potential in multiple applications.
Acacia catechu seed extract provokes cytotoxicity via apoptosis by intrinsic pathway in HepG2 cells

Thangavelu L, Geetha RV, Devaraj E, Dua K, Chellappan DK, Balusamy SR

Environ Toxicol, 2022 Mar;37(3):446-456.
PMID: 34800081 DOI: 10.1002/tox.23411

Acacia catechu Willd (Fabaceae) is a thorny tree widely distributed in India and commonly used as traditional Ayurvedic medicine for various ailments. The current study evaluates the cytotoxic potentials of A. catechu ethanolic seed extract (ACSE) in HepG2 cells, a human hepatocellular carcinoma cell line. The HepG2 cells were treated with 0.1, 0.3, 1, 3, 10, 30, 100, 300 and 1000 μg/ml of ACSE and the cytotoxic effect was evaluated by MTT and lactate dehydrogenase (LDH) leakage assays. The IC50 of ACSE was found at 77.04 μg/ml and therefore, further studies were carried out with the concentrations of 35 and 70 μg/ml. The intracellular reactive oxygen species (ROS) generation and apoptosis-related morphological changes were evaluated. Gene expressions of Bax, Bcl-2, cytochrome C (Cyt-c), caspases-9 and 3 were analyzed by qPCR. The ACSE treatments caused LDH leakage was associated with an increased ROS generation. The increased ROS generation was associated with the downregulation of intracellular antioxidant enzyme superoxide dismutase and reduced glutathione content. AO/EB and PI staining also confirmed chromatin condensation and apoptosis. The flow cytometric analysis showed an accumulation of HepG2 cells at sub G0/G1 (apoptotic) phase upon ACSE treatments. The ACSE induced cytotoxicity and oxidative stress were related to increased apoptotic marker gene expressions such as Bax, Cyt-c, caspase-9 and 3, and decreased anti-apoptotic marker Bcl-2. The current finding suggests that ACSE has apoptosis-inducing potential via the mitochondrial pathway in HepG2 cells.

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links