Penentuan kumpulan bangsa dalam analisis rambut pada kepala adalah satu cabaran yang dihadapi dalam analisis forensik untuk mengenal pasti mangsa, suspek dan tempat kejadian jenayah. Kajian ini bertujuan untuk mengenal pasti perbezaan antara dua kaum terbesar di Malaysia iaitu Melayu dan Cina melalui penelitian ciri-ciri rambut. Ini termasuklah ciri umum rambut, corak kutikel, warna, taburan pigmen kortek dan ciri medula – jenis, ketebalan dan indeks medula. Kajian ini telah dijalankan dengan menggunakan mikroskop cahaya dan terdiri daripada lima langkah iaitu penyediaan sampel, penelitian ciri umum rambut, penyediaan dan penelitian slaid acuan corak sisik kutikel, slaid kortek serta medula. Hasil daripada kajian in mendapati bahawa kebanyakan Melayu mempunyai warna pigmen yang bercoklat gelap manakala Cina mempunyai pigmen coklat cerah. Kami juga mendapati bahawa ketebalan medula boleh juga digunakan untuk membezakan antara Melayu dan Cina di mana nilai purata bagi Melayu dan Cina masing-masing adalah 7.59 ± 1.45 μm dan 9.12 ± 1.85 μm. Nilai purata indeks medula bagi Melayu dan Cina pula adalah 0.17 ± 0.02 μm dan 0.18 ± 0.02 μm masing-masing. Walaupun begitu, ciri-ciri seperti tekstur, ketebalan rambut, corak sisik kutikel, taburan pigmen dan jenis medula pula menunjukkan perbezaan yang tidak ketara antara Melayu dan Cina. Kesimpulannya, kajian ini telah menunjukkan bahawa warna pigmen, ketebalan medula dan indeks medula adalah ciri-ciri penting yang boleh digunakan untuk menentukan bangsa.
Red seaweed Gracilaria, one of the largest genus in Division Rhodophyta inhabits Sarawak coastal water. This study was designed to identify the species of Gracilaria using morphological approach and to assess selected water quality parameters in Gracilaria habitats. Three field samplings were carried out in Santubong and Asajaya, Sarawak from November 2013 to December 2014. Overall, three species were identified namely Gracilaria changii, G. blodgettii and G. coronopifolia, attached to net of cage culture in Santubong and root of mangrove trees in Asajaya. In addition, three different taxa of aquatic macroinvertebrates (polychaete, small crab, bivalve) and single species of red seaweed (Acanthophora sp.) were observed in Gracilaria assemblages. An estimate of 37% to 40% of the upper part of the cage net in Santubong was covered by seaweeds and only 16% to 20% in Asajaya's mangrove. The study had provided better information on identification of Gracilaria and their habitat in Sarawak. Future work involving DNA barcoding of each species is in progress.
Benthic faunal communities are important components in the intertidal zones.
The diversity and abundance of the benthic communities are subjected to different natural
and anthropogenic disturbances. The study was conducted as one off sampling on 6th
November 2013 (1) to investigate the abundance and distribution of soft sediment
communities in relation to environmental variables and (2) investigate the changes of
population structure and diversity using spatial scales of 1 m, 10 m, and 100 m. Results
indicated a total of 110 individuals of macrobenthos consisting of 7 different groups
(Annelida, Bivalvia, Crustacea, Gastropoda, Nematoda, Nemertea, Polychaeta) and 4
different groups of meiobenthos (Copepoda, Nematoda, Ostracoda, Polychaeta)
consisting 920 individuals were recorded. Dissolved oxygen played the most significant
role in affecting the distribution of soft sediment communities while ammonia
concentrations only affected marcobenthic organisms. However, sediment grain size did
not show significant correlation (p>0.05) on soft sediment communities. Hence,
understanding how different properties of benthos respond to changes in environmental
variables is crucial in determining how the impacts on the sediment are tolerated by the
benthic organisms.
Angelwing clam (Pholas orientalis) hydrolysate was prepared by hydrolysis using bromelain. The hydrolysate named as bromelain hydrolysate (BH) was then treated with β-cyclodextrin in the ratio of 1:0.8 (v/w) by physical mixing and kneading methods producing the physical mixed hydrolysate (PMH) and kneaded method hydrolysate (KMH), respectively. The masking effect of β-cyclodextrin on bitterness was evaluated based on sensory analysis, amino acid analysis and determination of flavor compound by gas chromatography- mass spectrometry (GC-MS) and field emission scanning electron microscope (FESEM). Sensory analysis showed that KMH has least bitter taste compared to BH. Amino acids analysis showed that hydrophobic amino acids content that contributed to the bitter taste were lower in KMH and PMH compared to BH. GC-MS analysis also showed that benzothiazole compounds were present in KMH. The absence of benzene, 1-phenyl-4-2-(2-cyano-2-phenylethyl) in KMH and PMH indicated that phenylalanine in BH had been masked by β-cyclodextrin. FESEM showed that the new solid phase formed by kneading method has a crystal structure which was completely different from the original morphology of BH and β-cyclodextrin. Therefore, the bitterness in BH had successfully been masked by β-cyclodextrin, thus indicates its potential to be used as food ingredient..
Physicochemical properties of mud clam (Polymesoda erosa) hydrolysate produced using two microbial enzymes; alcalase and flavourzyme were determined. Hydrolysis using alcalase at 20.28% degree of hydrolysis (DH) resulted in 25.06 % yield and 45.37% protein while flavourzyme hydrolysis showed 22.93 % DH, 46.67 % protein and 30.68 % yield. Both hydrolysates were yellowish. Better emulsifying properties, foaming properties and water and oil holding capacity were exhibited by flavourzyme hydrolysate compared to the alcalase hydrolysate. However, in terms of amino acid composition, alcalase hydrolysate contained higher amino acid composition (75.06%) than flavourzyme hydrolysate (62.37%). The study suggested that mud clam hydrolysate had the potential to be used in food formulations for human consumption.
In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties
Pengecaman senjata api semakin serius dan amat penting di dalam bidang penyiasatan jenayah khususnya untuk tempoh dua dekad ini. Dalam makalah ini, suatu sistem untuk pengecaman senjata api berasaskan tapak kelongsong peluru telah diperkenalkan. Tapak kelongsong peluru adalah satu daripada penunjuk yang amat penting dalam membantu menyelesaikan masalah pengecaman pistol. Peluru yang telah digunakan akan meninggalkan lebih daripada 30 ciri yang amat berharga pada tapak kelongsong bagi membantu pihak tertentu mengecam pistol yang telah diguna. Ciri-ciri tersebut sebagai suatu gabungan membentuk apa yang dikenali sebagai kesan cap jari bagi pistol. Lantaran itu, kajian ini adalah untuk mendapatkan fitur yang sesuai bagi pengecaman senjata api. Terlebih dahulu imej tapak kelongsong peluru ini telah ditemberengkan kepada tiga bahagian, membentuk tiga set imej yang berlainan. Imej-imej ini juga dilakukan prapemprosesan untuk membentuk tiga set imej lagi. Fitur-fitur seterusnya disaring daripada imej asal tapak kelongsong peluru dan imej yang telah melalui prapemprosesan. Dua puluh fitur yang berbeza secara signifikan telah diperoleh dan dikirakan untuk imej-imej asal dan yang telah dilakukan prapemprosesan. Kesemua pemprosesan telah dilakukan menggunakan pengaturcaraan MATLAB. Suatu skim berdasarkan analisis korelasi seterusnya telah diperkenalkan untuk pencarian fitur berdasarkan konsep meminimumkan lewahan data tetapi memastikan ciri-ciri unik tersimpan. Fitur-fitur yang berkorelasi tinggi akan digugurkan pasangannya dan hasilnya tinggal cuma tujuh fitur sahaja. Ketujuh-tujuh fitur ini telah diuji sebagai suatu vektor fitur untuk mengelaskan lima pistol daripada model yang sama menggunakan analisis diskriminan. Hasil pengelasan menunjukkan lebih 80% daripada imej-imej tapak kelongsong peluru itu telah dikelaskan dengan jayanya.
Recruitment constraints on Singapore's dwindling fluted giant clam, Tridacna squamosa, population were studied by modelling fertilisation, larval transport, and settlement using real-time hydrodynamic forcing combined with knowledge of spawning characteristics, larval development, behaviour, and settlement cues. Larval transport was simulated using a finite-volume advection-diffusion model coupled to a three-dimensional hydrodynamic model. Three recruitment constraint hypotheses were tested: 1) there is limited connectivity between Singapore's reefs and other reefs in the region, 2) there is limited exchange within Singapore's Southern Islands, and 3) there exist low-density constraints to fertilisation efficacy (component Allee effects). Results showed that connectivity among giant clam populations was primarily determined by residual hydrodynamic flows and spawning time, with greatest chances of successful settlement occurring when spawning and subsequent larval dispersal coincided with the period of lowest residual flow. Simulations suggested poor larval transport from reefs located along the Peninsular Malaysia to Singapore, probably due to strong surface currents between the Andaman Sea and South China Sea combined with a major land barrier disrupting larval movement among reefs. The model, however, predicted offshore coral reefs to the southeast of Singapore (Bintan and Batam) may represent a significant source of larvae. Larval exchange within Singapore's Southern Islands varied substantially depending on the locations of source and sink reefs as well as spawning time; but all simulations resulted in low settler densities (2.1-68.6 settled individuals per 10,000 m(2)). Poor fertilisation rates predicted by the model indicate that the low density and scattered distribution of the remaining T. squamosa in Singapore are likely to significantly inhibit any natural recovery of local stocks.
<b>Background and Objective:</b> Bamboo clam <i>Solen lamarckii</i> is one of the bivalve's species that have important economic value in Indonesian waters. Knowledge of the reproductive biology of a species is essential for proper and sustainable fisheries management. Monthly variations in the gonad maturity level of the clams from the coastal waters of Bengkalis Island Indonesia, were examined. <b>Materials and Methods:</b> Samples of the clam were collected monthly at the intertidal zone during low tide from May to September, 2020. A total of 239 specimens of which 116 females and 123 males were examined. Reproductive aspects observed in this study included sex ratio, gonadosomatic index, monthly gonadal maturity level and female fecundity. Mature female or near spawning phases were used for fecundity estimation. The sex ratios were analysed using a Chi-quadrat-Test, whereas to determine differences in fecundity between female individuals tested by Analysis of Variance. <b>Results:</b> Based on the gonadosomatic index (GSI) and histological observation of both sexes's gonads, it can be concluded that early spawning occurred in August and the peak seemed to take place during October-November. The sex ratios were analysed using a Chi-quadrat-Test which was not different from parity. Gonad maturation time between males and females indicates synchrony and no hermaphrodites were observed in the collection. Females shell length range of 75.5 to 89.3 mm produced 4.63×103-7.43×103 eggs ind-1 female (N = 10). Females with a shell length between 75.5 and 89.3 mm had varying fecundities, but analysis of variance showed no significant difference (Sig>0.05). <b>Conclusion:</b> Based on the gonadosomatic index (GSI) and histological analysis, <i>S. lamarckii</i> in Bengkalis waters spawned in August. Males and females showed spawning synchrony.
We analyzed 68 green and blue mussels collected from Cambodia, China, Hong Kong, India, Indonesia, Japan, Korea, Malaysia, Philippines, Vietnam and the USA during 2003 and 2007, to elucidate the occurrence and widespread distributions of emerging pollutants, synthetic musks and benzotriazole UV stabilizers (BUVSs) in Asia-Pacific coastal waters. Synthetic musks and BUVSs were detected in mussels from all countries, suggesting their ubiquitous contamination and widespread distribution. High concentrations of musks and BUVSs were detected in mussels from Japan and Korea, where the levels were comparable or greater than those of PCBs, DDTs and PBDEs. Significant correlations were found between the concentrations of HHCB and AHTN, and also between the concentrations of UV-327 and UV-328, which suggest similar sources and compositions of these compounds in commercial and industrial products. To our knowledge, this is the first study of large-scale monitoring of synthetic musks and BUVSs in Asia-Pacific coastal waters.
Tabiat merokok adalah perilaku yang tidak sihat malah membahayakan bukan sahaja diri sendiri malahan masyarakat sekeliling. Walaupun terdapat banyak kempen anti-merokok telah diperkenalkan oleh kerajaan, namun keberkesanannya masih di tahap rendah. Oleh itu objektif kajian ini adalah untuk meneroka faktor utama kegagalan kempen anti-merokok di Malaysia, meneroka keberkesanan “Klinik Berhenti Merokok” dan menemui penyelesaian terbaik untuk memastikan kempen yang dijalankan berkesan pada masa akan datang. Ini adalah kajian rentas kualitatif di mana temuduga dijalankan di kalangan lima sukarela responden yang terdiri dari perokok dan bekas perokok di kawasan awam dan tiga klinik berhenti merokok yang terpilih. Data dianalisis dengan menggunakan analisis bertema. Kajian ini mendapati beberapa faktor penyumbang dari perspektif perokok dan pihak berkuasa perlu diberi keutamaan untuk penambahbaikan iaitu kurang kreativiti yang minimum dalam menguruskan promosi anti-merokok, kurang kreatitivi dah terhadap kesihatan, permintaan bahan pencetus semangat di tempat kerja, dan dalam pengurusan tekanan yang lemah. Hasil kajian mengesyorkan bahawa kempen bermula di sekolah rendah dan menengah, improvisasi tanda amaran penyakit, meluaskan kawasan promosi, memberi ganjaran semasa kempen dan perokok perlu mengambil inisiatif melibatkan diri dalam program ini. Kerajaan perlu menumpukan perhatian kepada program pencegahan merokok terlebih dahulu, daripada program intervensi berhenti merokok yang mungkin melibatkan kos kewangan yang tinggi.
Fosil radiolaria berusia Perm Awal telah ditemui daripada batuan silika-rijang di Pos Blau, baratdaya Kelantan,
Semenanjung Malaysia. Sebanyak empat puluh spesies radiolaria tergolong dalam lima belas genera dan tujuh famili
telah dikenal pasti. Fauna radiolaria yang diperoleh termasuklah spesies penunjuk usia seperti Pseudoalbaillella
lomentaria, Ps. sakmarensis, Ps. scalprata scalprata, Ps. scalprata postscalprata dan Ps. scalprata rhombothoracata.
Kesemua radiolaria ini mewakili usia Perm awal merujuk kepada Zon Himpunan Pseudoalbaillella lomentaria
(Sakmarian Awal) dan Zon Himpunan Pseudoalbaillella scalprata m. rhombothoracata (Sakmarian Akhir). Keputusan
ini dapat menambah baik maklumat biostratigrafi radiolaria dan usia batuan bersilika-rijang di kawasan Pos Blau
dan di dalam Zon Sutura Bentong-Raub.
Contamination of persistent organochlorines (OCs) such as PCBs (polychlorinated biphenyls), DDT and its metabolites (DDTs), HCH (hexachlorocyclohexane) isomers (HCHs), chlordane compounds (CHLs), and HCB (hexachlorobenzene) were examined in mussels collected from coastal waters of Asian countries such as Cambodia, China, Hong Kong, India, Indonesia, Japan, Korea, Malaysia, Philippines, Far East Russia, Singapore, and Vietnam in 1994, 1997, 1998, 1999, and 2001 to elucidate the contamination status, distribution and possible pollution sources and to assess the risks on aquatic organisms and human. OCs were detected in all mussels collected from all the sampling sites investigated. Considerable residue levels of p,p(')-DDT and alpha-HCH were found in mussels and the concentrations of DDTs and HCHs found in mussels from Asian developing countries were higher than those in developed nations suggesting present usage of DDTs and HCHs along the coastal waters of Asian developing countries. On the other hand, lower concentrations of PCBs detected in mussels from Asian developing countries than those in developed countries indicate that PCBs contamination in mussels is strongly related to industrial and activities. To our knowledge, this is a first comprehensive report on monitoring OCs pollution in the Asia-Pacific region.
Suatu kajian untuk menilai kesan gabungan aktiviti guna tanah dan perubahan musim terhadap kualiti sumber air telah dijalankan di Terusan Utara, Kedah. Kajian ini dijalankan di lima stesen terpilih selama 12 bulan berdasarkan Indeks Kualiti Air (WQI) dan Piawaian Interim Kualiti Air Kebangsaan (INWQS). Kajian mendapati kualiti air di Terusan Utara berada pada Kelas III dan berlaku peningkatan kualiti air dari hulu ke hilir. Hampir semua stesen berpotensi sebagai punca pencemaran berdasarkan aktiviti guna tanah terutamanya di Pelubang, Jitra serta Tunjang. Kajian menunjukkan terdapat perbezaan yang signifikan ketika perubahan musim pada DO, TSS serta BOD, namun tidak pada ammonia, pH dan COD. Secara amnya, kombinasi aktiviti guna tanah dan perubahan musim boleh mempengaruhi atau memberi kesan terhadap kualiti sumber air. Oleh itu, tindakan segera perlu dilakukan untuk mengawal punca pencemaran bagi memastikan kualiti sumber air di Terusan Utara kekal terpelihara.
Kajian ini menemui faktor-faktor cabaran kempen anti-merokok dari perspektif petugas kesihatan di Kuantan. Objektif kajian ini adalah untuk menerokai cabaran kempen anti-merokok daripada pandangan petugas kesihatan, kesedaran tentang kewujudan Klinik Berhenti Merokok dan cara untuk menambah baik kempen antimerokok. Kajian ini adalah penting untuk membantu pihak berkuasa Malaysia untuk membangunkan strategi yang lebih baik dalam mempromosikan kempen anti-merokok pada masa akan datang. Persampelan “purposive” digunakan dalam pengumpulan data dan wawancara separa berstruktur telah dilakukan di kalangan lima petugas kesihatan dari tiga Klinik Berhenti Merokok yang terpilih. Kajian ini mendapati petugas kesihatan berpendapat bahawa perokok yang menerima rawatan dari Pusat Kesihatan mempunya pemahaman yang rendah mengenai tatacara piawai (SOP) dalam pemberhentian merokok, komitmen yang rendah dan senang dipengaruhi oleh masyarakat. Petugas kesihatan juga berpendapat tugas pelbagai disiplin menyebabkan tumpuan yang rendah terhadap pesakit yang merokok. Di samping itu, responden merasakan bahawa kewujudan Klinik Berhenti Merokok perlu diperluaskan penghebahannya kepada komuniti. Melalui penemuan ini, beberapa strategi dicadangkan untuk menambah baik pendekatan semasa dalam pengurangan bilangan perokok di Malaysia seperti penambahbaikan penghebahan kepada orang ramai, meningkatkan penguatkuasaan undang-undang serta menyediakan insentif.
Formasi Singa yang tersingkap di Kepulauan Langkawi terdiri daripada jujukan batuan sedimen argilit dan boleh dibahagikan kepada empat ahli iaitu Rebak, Kentut, Ular dan Selang. Formasi Singa kaya dengan fosil terutamanya daripada jenis brakiopod, bryozoa dan bivalvia yang boleh ditemui dalam Ahli Rebak dan Ahli Selang. Fosil yang ditemui semasa kajian dijalankan serta fosil yang pernah dilaporkan oleh penyelidik terdahulu telah dibuat penelitian semula untuk menentukan usia Formasi Singa dengan lebih tepat. Himpunan fosil Formasi Singa boleh dibahagikan kepada tiga julat usia, iaitu Visean, Asselian Akhir - Sakmarian Awal dan Sakmarian Akhir. Jujukan berusia Visean diwakili oleh Ahli Rebak, manakala jujukan Asselian Akhir - Sakmarian Awal dan jujukan Sakmarian Akhir diwakili oleh Ahli Selang. Dalam Ahli Ular dan Ahli Kentut tidak ditemui sebarang fosil. Keputusan daripada kertas ini memerlukan satu kajian semula dijalankan terhadap litostratigrafi Formasi Singa.
The purpose of this paper was to provide a practical guide assisting field workers in identification and interpretation of frequently occurring bioerosional textures created in limestone by intertidal organisms along the coasts of Langkawi Islands, Malaysia. The discussion follows the textural succession from the supratidal down to the lower intertidal zone. Traces left by lichens, boring sponges, molluscs (littorinid snails, the chiton Acanthopleura, the bivalve Lithophaga) and the echinoid Echinometra are illustrated. Products of bioconstructing organisms, specifically oysters and barnacle are also described. Ecological tolerance of each group is given.
Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody clams to non-food contact surfaces (hands and kitchen utensils) to lettuce (via slicing), was evaluated; 2) perfunctory decontamination, the efficacy of two superficial cleaning treatments: a) rinsing in a pail of water, and b) wiping with a kitchen towel, were determined; and 3) secondary cross-contamination, the microbial transfer from cleaning residuals (wash water or stained kitchen towel) to lettuce was assessed. The mean of percent transfer rates through direct contact was 3.6%, and an average of 3.5% of total V. parahaemolyticus was recovered from sliced lettuce. The attempted treatments reduced the transferred population by 99.0% (rinsing) and 94.5% (wiping), and the relative amount of V. parahaemolyticus on sliced lettuce was reduced to 0.008%. V. parahaemolyticus exposure via secondary cross-contamination was marginal. The relative amount of V. parahaemolyticus recovered from washed lettuce was 0.07%, and the transfers from stained kitchen towel to lettuce were insubstantial. Our study highlights that V. parahaemolyticus was readily spread in the kitchen, potentially through sharing of non-food contact surfaces. Results from this study can be used to better understand and potentially raising the awareness of proper handling practices to avert the spread of foodborne pathogens.
Two Unionida (freshwater mussel) families are present in the Northern Hemisphere; the Margaritiferidae, representing the most threatened of unionid families, and the Unionidae, which include several genera of unresolved taxonomic placement. The recent reassignment of the poorly studied Lamprotula rochechouartii from the Unionidae to the Margaritiferidae motivated a new search for other potential species of margaritiferids from members of Gibbosula and Lamprotula. Based on molecular and morphological analyses conducted on newly collected specimens from Vietnam, we here assign Gibbosula crassa to the Margaritiferidae. Additionally, we reanalyzed all diagnostic characteristics of the Margaritiferidae and examined museum specimens of Lamprotula and Gibbosula. As a result, two additional species are also moved to the Margaritiferidae, i.e. Gibbosula confragosa and Gibbosula polysticta. We performed a robust five marker phylogeny with all available margaritiferid species and discuss the taxonomy within the family. The present phylogeny reveals the division of Margaritiferidae into four ancient clades with distinct morphological, biogeographical and ecological characteristics that justify the division of the Margaritiferidae into two subfamilies (Gibbosulinae and Margaritiferinae) and four genera (Gibbosula, Cumberlandia, Margaritifera, and Pseudunio). The systematics of the Margaritiferidae family is re-defined as well as their distribution, potential origin and main biogeographic patterns.