MyMedR

Displaying publications 61 - 80 of 162 in total

Abstract:

Sort:

Fulltext Enzyme-Assisted Discovery of Antioxidant Peptides from Edible Marine Invertebrates: A Review

Chai TT, Law YC, Wong FC, Kim SK

Mar Drugs, 2017 Feb 16;15(2).
PMID: 28212329 DOI: 10.3390/md15020042

Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates. Antioxidant peptides have potential applications in food, pharmaceuticals and cosmetics. In this review, we first give a brief overview of the current state of progress of antioxidant peptide research, with special attention to marine antioxidant peptides. We then focus on 22 investigations which identified 32 antioxidant peptides from enzymatic hydrolysates of edible marine invertebrates. Strategies adopted by various research groups in the purification and identification of the antioxidant peptides will be summarized. Structural characteristic of the peptide sequences in relation to their antioxidant activities will be reviewed. Potential applications of the peptide sequences and future research prospects will also be discussed.

Matched MeSH terms: Seafood
Fulltext Evaluation of cloves (Syzygium aromaticum) against antibiotics resistant Vibrio parahaemolyticus on seafood

Elexson, N., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M.

International Food Research Journal, 2016;23(5):2229-2233.
MyJurnal

Vibrio parahaemolyticus is a main foodborne disease in seafood and generally seafood is
easily deteriorates in quality of color and flavor. In this study, clove (Syzygium aromaticum)
extract shows potent antibacterial activity against growth of antibiotics resistant Vibrio
parahaemolyticus on seafood samples (cockles and shrimps). Vibrio parahaemolyticus was
artificial contaminates on the samples with 106 CFU/ml. The samples were treated with different
concentration of cloves extract with 10 mg/ml which are 0.5%, 5% and 10% concentration
from methanol food grade extraction in 0 hr, 5 min, 10 min, 15 min, 30 min, 60 min and
120 min. Tab water and deionized water were selected as a negative control. As a result, the
amount of 10 % cloves managed to mitigates the number of V. parahaemolyticus on seafood
samples in 5 minutes and 15 min on both samples. Therefore, our results signify the fact that
cloves can be apply as natural sanitizer which could meet consumer demands for safe and
traditionally consumed either raw without any undesirable effect when applied in the seafood
system industries.

Matched MeSH terms: Seafood
Fulltext Evaluation of umaminess in green mussel hydrolysate (Perna viridis) produced in the presence of sodium tripolyphosphate and NaCl

Normah, I., Nurdalila Diyana, M.R.

International Food Research Journal, 2018;25(6):524-530.
MyJurnal

This study was conducted to evaluate umami taste in protein hydrolysate produced from green mussel (Perna viridis) by hydrolysing with flavorzyme at pH 8, enzyme substrate ratio (E/S) 3% with or without the presence of 0.4% sodium tripolyphosphate (STPP) and 1.5% NaCI. Degree of hydrolysis (DH), yield, amino acid compositions, molecular weight distribution and sensory evaluation were determined. The highest DH (23.18%), darkest color and highest yield (8.34%) were recorded for hydrolysate produced in the presence of both STPP and NaCI. Electrophoresis analysis showed the presence of protein bands between 10 to 70 kDa where hydrolysate with addition of STPP and NaCI had bands with lower intensities. Amino acids which contribute to the umami taste such as glutamic acid, glycine and aspartic acid were higher in hydrolysate produced with STPP and NaCI addition. The hydrolysate has lesser fishy odor and flavor than those produced with only in the presence of flavorzyme and was also rated with highest score for all the five basic tastes including bitterness. However, the score for bitterness was still lower than the reference solutions. Therefore, green mussel hydrolysate produced in this study has a good potential as a food flavorant.

Matched MeSH terms: Seafood
Fulltext Evidence of Marine Microplastics in Commercially Harvested Seafood

Curren E, Leaw CP, Lim PT, Leong SCY

Front Bioeng Biotechnol, 2020;8:562760.
PMID: 33344429 DOI: 10.3389/fbioe.2020.562760

Microplastic pollution is a global issue that has a detrimental impact on food safety. In marine environments, microplastics are a threat to marine organisms, as they are often the same size range as prey and are mistaken as food. Consumption of microplastics has led to the damage of digestive organs and a reduction in growth and reproductive output. In this study, microplastic pollution was assessed across three commercially available shrimp species that were obtained from the supermarkets of Singapore. A total of 93 individuals were studied from the Pacific white leg shrimp, Litopenaeus vannamei, the Argentine red shrimp Pleoticus muelleri and the Indian white shrimp Fenneropenaeus indicus. Microplastic fibers, fragments, film and spheres were identified from the digestive tract of these organisms. Microplastic abundance ranged from 13.4 to 7050 items. F. indicus exhibited the highest number of microplastics. Microplastic film was the most abundant in L. vannamei individuals (93-97%) and spheres were the most abundant in P. muelleri (70%) and F. indicus (61%) individuals. This study demonstrates that microplastic contamination is evident in commonly consumed shrimp and highlights the role of shrimp in the trophic transfer and accumulation of microplastics in seafood. The consumption of microplastic-containing seafood is a route of exposure to humans and has implications on human health and food security. Capsule: Microplastics were examined in three shrimp species from the supermarkets of Singapore. Microplastics ranged from 13.4 to 7050 items of shrimp.

Matched MeSH terms: Seafood
Fulltext Exposure Assessment of methyl mercury from consumption of fish and seafood in Peninsular Malaysia

Ahmad NI, Mahiyuddin WRW, Azmi WNFW, Azlee RSR, Shaharudin R, Sulaiman LH

Environ Sci Pollut Res Int, 2022 Apr;29(17):24816-24832.
PMID: 34826068 DOI: 10.1007/s11356-021-17483-6

The concentration of meHg in freshwater fish and seafood was investigated, as well as the consumption patterns of fish and seafood by different demographic groups (age, ethnicity, gender). A potential alarm for human health hazards was also assessed, and the results were compared to the provisional tolerable weekly intakes (PTWIs) and the hazard quotient parameter (HQ). The results showed that meHg levels of 67 species ranged from 0.013 to 0.252 mg/kg of wet weight (WW) with significant differences between different fish and seafood groups (χ2KW = 49.09; p seafood groups in descending orders are as follows: demersal fish (0.1006 mg/kg WW) > pelagic fish (0.0686 mg/kg WW) > freshwater fish 0.045 mg/kg WW) > cephalopods (0.0405 mg/kg WW) crustaceans (0.0356 mg/kg WW). The results revealed that older population (> 40 years old) consumed significantly (p = 0.000) more fish compared to younger generations and the elderly consumed the highest amounts of fish (104.0 ± 113.0 g/day). The adolescents (10-17 years old) consumed more than double of amount for both cephalopod and crustacean compared to the older populations (p seafood compared to other ethnicities, similar to male subjects (95.2 ± 102 g/day; p = 0.026) when compared to the female (86 ± 96.3 g/day). The estimated weekly intake (EWI) values showed results below 1.6 µg/kg BW/week, the tolerable levels recommended by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) for all different demographic factors except for higher consumers at 75th percentile and above. Consumption of marine fish contributed to a higher value of PTWI to all different demographic groups (the estimated weekly intake (EWI) range: 0.2988-0.6893 µg/kg BW/week) but for the adolescents, where from the consumption of crustaceans (0.3488 µg/kg BW/week or 21.8% of PTWI) and cephalopods (0.504 µg/kg BW/week or 31.5% of PTWI). The results from this study also revealed the HQ value for overall consumption of fish and seafood by the adolescents and elderly exceeded one. This was contributed from the consumption of demersal fish and cephalopods, thus indicating the nonacceptable level of noncarcinogenic adverse health effects.

Matched MeSH terms: Seafood/analysis
Fulltext Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett-Burman and response surface methodological approaches

Fan H, Dumont MJ, Simpson BK

J Food Sci Technol, 2017 Nov;54(12):4000-4008.
PMID: 29085142 DOI: 10.1007/s13197-017-2864-5

Gelatin from salmon (Salmo salar) skin with high molecular weight protein chains (α-chains) was extracted using trypsin-aided process. Response surface methodology was used to optimise the extraction parameters. Yield, hydroxyproline content and protein electrophoretic profile via sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of gelatin were used as responses in the optimization study. The optimum conditions were determined as: trypsin concentration at 1.49 U/g; extraction temperature at 45 °C; and extraction time at 6 h 16 min. This response surface optimized model was significant and produced an experimental value (202.04 ± 8.64%) in good agreement with the predicted value (204.19%). Twofold higher yields of gelatin with high molecular weight protein chains were achieved in the optimized process with trypsin treatment when compared to the process without trypsin.

Matched MeSH terms: Seafood
Fulltext Fat content and EPA and DHA levels of selected marine, freshwater fish and shellfish species from the east coast of Peninsular Malaysia

Wan Rosli W. I., Rohana, A. J., Gan, S. H., Noor Fadzlina, H., Rosliza, H., Helmy, H., et al.

International Food Research Journal, 2012;19(3):815-821.
MyJurnal

The total lipid contents and the concentration of eicosapentaenoic (EPA) and docosahexaenoic
(DHA) fatty acid of fourteen selected marine, three freshwater fish species, four shellfish species and two selected canned fish species of East Coast of Peninsular Malaysia were determined. The fat content of all samples ranged from 1.01 % to 15.83 % with silver catfish reported to have the highest value. Most of the fish had fat amounts lower than 10% of their total weight. In general, DHA concentrations (50.50-165.21 µg/g) were significantly higher than EPA (11.12-55.38 µg/g) in all of the fish species analyzed. Among all marine fish species, the sixbar grouper recorded the highest concentration of DHA (165.21 µg/g) while barramundi had the highest concentration of EPA (55.38 µg/g). In conclusion, all fish and shellfish species are considered a good source of EPA and DHA, representing a very valuable essential nutrient for maintenance of human health.

Matched MeSH terms: Seafood
Fulltext First record of the Gloiopotes huttoni (Thomson, 1890) (Copepoda: Caligidae) parasitic on the swordfish Xiphias gladius along the southeast coast of India

Karthick Rajan D, Ravichandran S, Venmathi Maran BA

J Parasit Dis, 2018 Sep;42(3):458-461.
PMID: 30166796 DOI: 10.1007/s12639-018-1013-z

Parasitic copepods infect a large number of fishes. In particular, the family Caligidae (Crustacea) comprises more than 450 species worldwide, some of which are harmful to cultured and wild fishes. Recently, we found a parasitic copepod on the host swordfish Xiphias gladius (Xiphidae) in the coastal waters of Parangipettai, southeast coast of India. It was identified as Gloiopotes huttoni by its morphological features, and it belongs to the family Caligidae (order Siphonostomatoida). The swordfish was highly infected with about 72 caligids on a single host. Both male and female forms of G. huttoni were colonized on the beak of the swordfish and distributed mostly along the upper and lower jaws of the host, with 42 and 30 copepod parasites, respectively. Most of the copepods were ovigerous, and more females than males were recorded. It is the first record of the occurrence of G. huttoni in India.

Matched MeSH terms: Seafood
Fulltext Fish nutrient composition: a review of global data from poorly assessed inland and marine species

Byrd KA, Thilsted SH, Fiorella KJ

Public Health Nutr, 2021 Feb;24(3):476-486.
PMID: 33308365 DOI: 10.1017/S1368980020003857

OBJECTIVE: Our understanding of the nutrient contribution of fish and other aquatic species to human diets relies on nutrient composition data for a limited number of species. Yet particularly for nutritionally vulnerable aquatic food consumers, consumption includes a wide diversity of species whose nutrient composition data are disparate, poorly compiled or unknown.
DESIGN: To address the gap in understanding fish and other aquatic species' nutrient composition data, we reviewed the literature with an emphasis on species of fish that are under-represented in global databases. We reviewed 164 articles containing 1370 entries of all available nutrient composition data (e.g. macronutrients, micronutrients and fatty acids) and heavy metals (e.g. Pb and Hg) for 515 species, including both inland and marine species of fish, as well as other aquatic species (e.g. crustaceans, molluscs, etc.) when those species were returned by our searches.
RESULTS: We highlight aquatic species that are particularly high in nutrients of global importance, including Fe, Zn, Ca, vitamin A and docosahexaenoic acid (DHA), and demonstrate that, in many cases, a serving can fill critical nutrient needs for pregnant and lactating women and young children.
CONCLUSIONS: By collating the available nutrient composition data on species of fish and other aquatic species, we provide a resource for fisheries and nutrition researchers, experts and practitioners to better understand these critical species and include them in fishery management as well as food-based programmes and policies.

Matched MeSH terms: Seafood/analysis*
Fulltext Food consumption and expenditures in Singapore: implications to Malaysia’s agricultural exports

Tey, Y.S., Suryani, D., Emmy, F.A., Illisriyani, I.

International Food Research Journal, 2009;16(2):119-126.
MyJurnal

Imports are the only source of food supply to Singapore. Such condition offers a very attractive proposition for agricultural oriented countries. The dependence on type of food imports is determined by consumer demand. This study intends to do an exploratory study on the current conditions of Singaporean food consumption and expenditures with implications on Malaysia as an important agricultural exporting country to Singapore. It is identified that Singaporean consumers tend to demand for high quality poultry, pork, seafood, vegetables, and fruits in future, particularly in response to income growth. At the mean time, Malaysia is seemingly rich in the production of these food commodities and commits to exports for the excess of the supplies. While facing competition from various countries, Malaysia still possesses various competitive advantages over the other countries.

Matched MeSH terms: Seafood
Geosesarma sodalis, a new species of vampire crab (Crustacea, Brachyura, Sesarmidae) from a limestone cave in central Sarawak, Malaysia

Ng PKL

Zookeys, 2021;1031:133-141.
PMID: 33958910 DOI: 10.3897/zookeys.1031.63134

A new species of semi-terrestrial crab of the genus Geosesarma (Sesarmidae) is described from a limestone cave in central Sarawak, Malaysian Borneo. Geosesarma sodalissp. nov. is characterised by its quadrate carapace, absence of a flagellum on the exopod of the third maxilliped, presence of 10 or 11 sharp tubercles on the dactylus of the chela and a diagnostic male first gonopod structure. This is the sixth species of Geosesarma reported from Sarawak, and the first member of the genus collected from inside caves.

Matched MeSH terms: Seafood
Glycemic responses of patients with type 2 diabetes to individual carbohydrate-rich foods and mixed meals

Robert SD, Ismail AA

Ann Nutr Metab, 2012;60(1):27-32.
PMID: 22212476 DOI: 10.1159/000335224

Our purpose was to determine whether the glycemic index (GI) of individual foods applies to mixed meals.

Matched MeSH terms: Seafood
Growth and epiphytic behavior of three Gambierdiscus species (Dinophyceae) associated with various macroalgal substrates

Mustapa NI, Yong HL, Lee LK, Lim ZF, Lim HC, Teng ST, et al.

Harmful Algae, 2019 Nov;89:101671.
PMID: 31672230 DOI: 10.1016/j.hal.2019.101671

Species of the benthic dinoflagellate Gambierdiscus produce polyether neurotoxins that caused ciguatera fish/shellfish poisoning in human. The toxins enter marine food webs by foraging of herbivores on the biotic substrates like macroalgae that host the toxic dinoflagellates. Interaction of Gambierdiscus and their macroalgal substrate hosts is believed to shape the tendency of substrate preferences and habitat specialization. This was supported by studies that manifested epiphytic preferences and behaviors in Gambierdiscus species toward different macroalgal hosts. To further examine the supposition, a laboratory-based experimental study was conducted to examine the growth, epiphytic behaviors and host preferences of three Gambierdiscus species towards four macroalgal hosts over a culture period of 40 days. The dinoflagellates Gambierdiscus balechii, G. caribaeus, and a new ribotype, herein designated as Gambierdiscus type 7 were initially identified based on the thecal morphology and molecular characterization. Our results showed that Gambierdiscus species tested in this study exhibited higher growth rates in the presence of macroalgal hosts. Growth responses and attachment behaviors, however, differed among different species and strains of Gambierdiscus over different macroalgal substrate hosts. Cells of Gambierdiscus mostly attached to substrate hosts at the beginning of the experiments but detached at the later time. Localized Gambierdiscus-host interactions, as demonstrated in this study, could help to better inform efforts of sampling and monitoring of this benthic toxic dinoflagellate.

Matched MeSH terms: Seafood
Health risk assessments of heavy metal exposure via consumption of marine mussels collected from anthropogenic sites

Yap CK, Cheng WH, Karami A, Ismail A

Sci Total Environ, 2016 May 15;553:285-96.
PMID: 26925739 DOI: 10.1016/j.scitotenv.2016.02.092

A total of 40 marine mussel Perna viridis populations collected (2002-2009) from 20 geographical sites located in two busy shipping lanes namely the Straits of Malacca (10 sites; 16 populations) and the Straits of Johore (8 sites; 21 populations) and three populations (2 sites) on the east coast of Peninsular Malaysia, was determined for Cd, Cu, Fe, Ni, Pb and Zn concentrations. In comparison with the maximum permissible limits (MPLs) set by existing food safety guidelines, all metal concentrations found in all the mussel populations were lower than the prescribed MPLs. In terms of the provisional tolerable weekly intake prescribed by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) and oral reference doses (ORDs) by the USEPA, all the studied metals (except for Pb) were unlikely to become the limiting factors or unlikely to pose a risk for the consumption of the mussel populations. The estimated daily intake (EDI) for average level mussel (ALM) and high level mussel (HLM) consumers of mussels was found to be lower than the ORD guidelines for Cd, Cu, Fe, Ni and Zn. Furthermore, the target hazard quotient (THQ) was found to be less than 1 for ALM consumers but higher than 1 for HLM consumers in some sites. Therefore, there were no potential human health risks to the ALM consumers of the mussels. However, for Pb THQ values, the Pb levels in some mussel populations could create a health risk problem. Present results indicate that the consumption amounts of mussels should be limited for minimizing potential health risks of heavy metals to the HLM consumers.

Matched MeSH terms: Seafood
Fulltext High-value components and bioactives from sea cucumbers for functional foods--a review

Bordbar S, Anwar F, Saari N

Mar Drugs, 2011;9(10):1761-805.
PMID: 22072996 DOI: 10.3390/md9101761

Sea cucumbers, belonging to the class Holothuroidea, are marine invertebrates, habitually found in the benthic areas and deep seas across the world. They have high commercial value coupled with increasing global production and trade. Sea cucumbers, informally named as bêche-de-mer, or gamat, have long been used for food and folk medicine in the communities of Asia and Middle East. Nutritionally, sea cucumbers have an impressive profile of valuable nutrients such as Vitamin A, Vitamin B1 (thiamine), Vitamin B2 (riboflavin), Vitamin B3 (niacin), and minerals, especially calcium, magnesium, iron and zinc. A number of unique biological and pharmacological activities including anti-angiogenic, anticancer, anticoagulant, anti-hypertension, anti-inflammatory, antimicrobial, antioxidant, antithrombotic, antitumor and wound healing have been ascribed to various species of sea cucumbers. Therapeutic properties and medicinal benefits of sea cucumbers can be linked to the presence of a wide array of bioactives especially triterpene glycosides (saponins), chondroitin sulfates, glycosaminoglycan (GAGs), sulfated polysaccharides, sterols (glycosides and sulfates), phenolics, cerberosides, lectins, peptides, glycoprotein, glycosphingolipids and essential fatty acids. This review is mainly designed to cover the high-value components and bioactives as well as the multiple biological and therapeutic properties of sea cucumbers with regard to exploring their potential uses for functional foods and nutraceuticals.

Matched MeSH terms: Seafood/analysis
Fulltext Higher fish but lower micronutrient intakes: Temporal changes in fish consumption from capture fisheries and aquaculture in Bangladesh

Bogard JR, Farook S, Marks GC, Waid J, Belton B, Ali M, et al.

PLoS One, 2017;12(4):e0175098.
PMID: 28384232 DOI: 10.1371/journal.pone.0175098

Malnutrition is one of the biggest challenges of the 21st century, with one in three people in the world malnourished, combined with poor diets being the leading cause of the global burden of disease. Fish is an under-recognised and undervalued source of micronutrients, which could play a more significant role in addressing this global challenge. With rising pressures on capture fisheries, demand is increasingly being met from aquaculture. However, aquaculture systems are designed to maximise productivity, with little consideration for nutritional quality of fish produced. A global shift away from diverse capture species towards consumption of few farmed species, has implications for diet quality that are yet to be fully explored. Bangladesh provides a useful case study of this transition, as fish is the most important animal-source food in diets, and is increasingly supplied from aquaculture. We conducted a temporal analysis of fish consumption and nutrient intakes from fish in Bangladesh, using nationally representative household expenditure surveys from 1991, 2000 and 2010 (n = 25,425 households), combined with detailed species-level nutrient composition data. Fish consumption increased by 30% from 1991-2010. Consumption of non-farmed species declined by 33% over this period, compensated (in terms of quantity) by large increases in consumption of farmed species. Despite increased total fish consumption, there were significant decreases in iron and calcium intakes from fish (P<0.01); and no significant change in intakes of zinc, vitamin A and vitamin B12 from fish, reflecting lower overall nutritional quality of fish available for consumption over time. Our results challenge the conventional narrative that increases in food supply lead to improvements in diet and nutrition. As aquaculture becomes an increasingly important food source, it must embrace a nutrition-sensitive approach, moving beyond maximising productivity to also consider nutritional quality. Doing so will optimise the complementary role that aquaculture and capture fisheries play in improving nutrition and health.

Matched MeSH terms: Seafood*
Fulltext Human Health Risk Assessments of Trace Metals on the Clam Corbicula javanica in a Tropical River in Peninsular Malaysia

Yap CK, Wong KW, Al-Shami SA, Nulit R, Cheng WH, Aris AZ, et al.

Int J Environ Res Public Health, 2020 12 29;18(1).
PMID: 33383875 DOI: 10.3390/ijerph18010195

This study aimed to analyse ten trace metal concentrations in the edible part of the freshwater clam Corbicula javanica and to provide a critical assessment of the potential risks to human health through consumption of this clam as food based on well-established indices and food safety guidelines. The clams were captured from a pristine original site and transplanted to other sites with different environmental qualities. The trace metal levels in the edible total soft tissue (TST) of the clam were below those of the food safety guidelines referred to except for Pb, which exceeded the permissible limit set by the European Commission (2006) and the US Food and Drug Administration/ Center for Food Safety and Applied Nutrition); Interstate Shellfish Sanitation Conference. (USFDA/CFSAN; ISSC) (2007). The estimated daily intake (EDI) values of the clam were found to be lower than the oral reference dose and the calculated target hazard quotient (THQ) and total THQ were found to be less than 1. Therefore, in conclusion, the human health risk for consumption of TST of C. javanica at both average and high-level were insignificant regardless of the environment it was exposed to.

Matched MeSH terms: Seafood*
Fulltext Identification of Fish Species and Toxins Implicated in a Snapper Food Poisoning Event in Sabah, Malaysia, 2017

Dao HV, Uesugi A, Uchida H, Watanabe R, Matsushima R, Lim ZF, et al.

Toxins (Basel), 2021 09 15;13(9).
PMID: 34564661 DOI: 10.3390/toxins13090657

In the coastal countries of Southeast Asia, fish is a staple diet and certain fish species are food delicacies to local populations or commercially important to individual communities. Although there have been several suspected cases of ciguatera fish poisoning (CFP) in Southeast Asian countries, few have been confirmed by ciguatoxins identification, resulting in limited information for the correct diagnosis of this food-borne disease. In the present study, ciguatoxin-1B (CTX-1B) in red snapper (Lutjanus bohar) implicated in a CFP case in Sabah, Malaysia, in December 2017 was determined by single-quadrupole selected ion monitoring (SIM) liquid chromatography/mass spectrometry (LC/MS). Continuous consumption of the toxic fish likely resulted in CFP, even when the toxin concentration in the fish consumed was low. The identification of the fish species was performed using the molecular characterization of the mitochondrial cytochrome c oxidase subunit I gene marker, with a phylogenetic analysis of the genus Lutjanus. This is the first report identifying the causative toxin in fish-implicated CFP in Malaysia.

Matched MeSH terms: Seafood/classification; Seafood/toxicity*
Fulltext Identification of major and minor allergens of black tiger prawn (Penaeus monodon) and king prawn (Penaeus latisulcatus)

Sahabudin S, Misnan R, Yadzir ZH, Mohamad J, Abdullah N, Bakhtiar F, et al.

Malays J Med Sci, 2011 Jul;18(3):27-32.
PMID: 22135598 MyJurnal

BACKGROUND: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species.
METHODS: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts.
RESULTS: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses.
CONCLUSION: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.
KEYWORDS: Penaeus; allergens; allergy and clinical immunology; hypersensitivity; immunoblotting; tropomyosin

Matched MeSH terms: Seafood
Investigating the biometric and physicochemical characteristics of freshly harvested Pacific white shrimp (Litopenaeus vannamei): a comparative approach

Okpala CO, Bono G

J Sci Food Agric, 2016 Mar 15;96(4):1231-40.
PMID: 25866918 DOI: 10.1002/jsfa.7211

The practicality of biometrics of seafood cannot be overemphasized, particularly for competent authorities of the shrimp industry. However, there is a paucity of relevant literature on the relationship between biometric and physicochemical indices of freshly harvested shrimp. This work therefore investigated the relationship between biometric (standard length (SL), total weight (TW) and condition factor (CF)) and physicochemical (moisture content, pH, titratable acidity, water activity, water retention index, colour values and fracturability) characteristics of freshly harvested Pacific white shrimp (Litopenaeus vannamei) obtained from three different farms. The relationships between these parameters were determined using correlation and regression analyses.

Matched MeSH terms: Seafood

Filters

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links