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Gene Therapy for Duchenne Muscular Dystrophy: Unlocking the Opportunities in Countries in the Middle East and Beyond

Elbashir H, Fathalla W, Mundada V, Iqbal M, Al Tawari AA, Chandratre S, et al.

J Neuromuscul Dis, 2022;9(6):787-801.
PMID: 36245386 DOI: 10.3233/JND-221528

BACKGROUND: Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder which leads to progressive muscle degeneration and weakness. Most patients die from cardiac or respiratory failure. Gene transfer therapy offers a promising approach to treating this disorder.
OBJECTIVE: Given the genetic disease burden, family size, and the high consanguinity rates in the Middle East, our objective is to address current practices and challenges of DMD patient care within two countries in this region, namely the United Arab Emirates and Kuwait, and to outline readiness for gene therapy.
METHODS: An expert panel meeting was held to discuss the DMD patient journey, disease awareness, current management of DMD, challenges faced and recommendations for improvement. Opportunities and challenges for gene therapy in both countries were also deliberated. A pre-meeting survey was conducted, and the results were used to guide the discussion during the meeting.
RESULTS: DMD awareness is poor resulting in a delay in referral and diagnosis of patients. Awareness and education initiatives, along with an interconnected referral system could improve early diagnosis. Genetic testing is available in both countries although coverage varies. Corticosteroid therapy is the standard of care however there is often a delay in treatment initiation. Patients with DMD should be diagnosed and managed by a multi-disciplinary team in centers of excellence for neuromuscular disorders. Key success factors to support the introduction of gene therapy include education and training, timely and accessible genetic testing and resolution of reimbursement and cost issues.
CONCLUSION: There are many challenges facing the management of DMD patients in the United Arab Emirates and Kuwait and most likely other countries within the Middle East. Successful introduction of gene therapy to treat DMD will require careful planning, education, capacity building and prioritization of core initiatives.

Matched MeSH terms: Genetic Therapy/methods
Fulltext Two-Dimensional Non-Carbon Materials-Based Electrochemical Printed Sensors: An Updated Review

Falina S, Anuar K, Shafiee SA, Juan JC, Manaf AA, Kawarada H, et al.

Sensors (Basel), 2022 Dec 01;22(23).
PMID: 36502059 DOI: 10.3390/s22239358

Recently, there has been increasing interest in electrochemical printed sensors for a wide range of applications such as biomedical, pharmaceutical, food safety, and environmental fields. A major challenge is to obtain selective, sensitive, and reliable sensing platforms that can meet the stringent performance requirements of these application areas. Two-dimensional (2D) nanomaterials advances have accelerated the performance of electrochemical sensors towards more practical approaches. This review discusses the recent development of electrochemical printed sensors, with emphasis on the integration of non-carbon 2D materials as sensing platforms. A brief introduction to printed electrochemical sensors and electrochemical technique analysis are presented in the first section of this review. Subsequently, sensor surface functionalization and modification techniques including drop-casting, electrodeposition, and printing of functional ink are discussed. In the next section, we review recent insights into novel fabrication methodologies, electrochemical techniques, and sensors' performances of the most used transition metal dichalcogenides materials (such as MoS2, MoSe2, and WS2), MXenes, and hexagonal boron-nitride (hBN). Finally, the challenges that are faced by electrochemical printed sensors are highlighted in the conclusion. This review is not only useful to provide insights for researchers that are currently working in the related area, but also instructive to the ones new to this field.

Matched MeSH terms: Electrochemical Techniques/methods
Utilizing 3D Slicer to incorporate tomographic images into GATE Monte Carlo simulation for personalized dosimetry in yttrium-90 radioembolization

Abdul Hadi MFR, Abdullah AN, Hashikin NAA, Ying CK, Yeong CH, Yoon TL, et al.

Med Phys, 2022 Dec;49(12):7742-7753.
PMID: 36098271 DOI: 10.1002/mp.15980

PURPOSE: Monte Carlo (MC) simulation is an important technique that can help design advanced and challenging experimental setups. GATE (Geant4 application for tomographic emission) is a useful simulation toolkit for applications in nuclear medicine. Transarterial radioembolization is a treatment for liver cancer, where microspheres embedded with yttrium-90 (90 Y) are administered intra-arterially to the tumor. Personalized dosimetry for this treatment may provide higher dosimetry accuracy compared to the conventional partition model (PM) calculation. However, incorporation of three-dimensional tomographic input data into MC simulation is an intricate process. In this article, 3D Slicer, free and open-source software, was utilized for the incorporation of patient tomographic images into GATE to demonstrate the feasibility of personalized dosimetry in hepatic radioembolization with 90 Y.
METHODS: In this article, the steps involved in importing, segmenting, and registering tomographic images using 3D Slicer were thoroughly described, before importing them into GATE for MC simulation. The absorbed doses estimated using GATE were then compared with that of PM. SlicerRT, a 3D Slicer extension, was then used to visualize the isodose from the MC simulation.
RESULTS: A workflow diagram consisting of all the steps taken in the utilization of 3D Slicer for personalized dosimetry in 90 Y radioembolization has been presented in this article. In comparison to the MC simulation, the absorbed doses to the tumor and normal liver were overestimated by PM by 105.55% and 20.23%, respectively, whereas for lungs, the absorbed dose estimated by PM was underestimated by 25.32%. These values were supported by the isodose distribution obtained via SlicerRT, suggesting the presence of beta particles outside the volumes of interest. These findings demonstrate the importance of personalized dosimetry for a more accurate absorbed dose estimation compared to PM.
CONCLUSION: The methodology provided in this study can assist users (especially students or researchers who are new to MC simulation) in navigating intricate steps required in the importation of tomographic data for MC simulation. These steps can also be utilized for other radiation therapy related applications, not necessarily limited to internal dosimetry.

Matched MeSH terms: Radiometry/methods
Fulltext Regression Analysis of the Dielectric and Morphological Properties for Porous Nanohydroxyapatite/Starch Composites: A Correlative Study

Beh CY, Cheng EM, Mohd Nasir NF, Abdul Majid MS, Khor SF, Mohd Jamir MR, et al.

Int J Mol Sci, 2022 May 19;23(10).
PMID: 35628505 DOI: 10.3390/ijms23105695

This paper aims to investigate the dielectric properties, i.e., dielectric constant (ε'), dielectric loss factor (ε″), dielectric tangent loss (tan δ), electrical conductivity (σ), and penetration depth (Dp), of the porous nanohydroxyapatite/starch composites in the function of starch proportion, pore size, and porosity over a broad band frequency range of 5 MHz-12 GHz. The porous nanohydroxyapatite/starch composites were fabricated using different starch proportions ranging from 30 to 90 wt%. The results reveal that the dielectric properties and the microstructural features of the porous nanohydroxyapatite/starch composites can be enhanced by the increment in the starch proportion. Nevertheless, the composite with 80 wt% of starch proportion exhibit low dielectric properties (ε', ε″, tan δ, and σ) and a high penetration depth because of its highly interconnected porous microstructures. The dielectric properties of the porous nanohydroxyapatite/starch composites are highly dependent on starch proportion, average pore size, and porosity. The regression models are developed to express the dielectric properties of the porous nanohydroxyapatite/starch composites (R2 > 0.96) in the function of starch proportion, pore size, and porosity from 1 to 11 GHz. This dielectric study can facilitate the assessment of bone scaffold design in bone tissue engineering applications.

Matched MeSH terms: Tissue Engineering/methods
Impact of age and breast thickness on mean glandular dose of standard digital mammography and digital breast tomosynthesis

Norsuddin NM, Mei Sin JG, Ravintaran R, Arasaratnam S, Abdul Karim MK

Appl Radiat Isot, 2023 Feb;192:110525.
PMID: 36436228 DOI: 10.1016/j.apradiso.2022.110525

This study compares the mean glandular dose (MGD) across 2D, 3D projection and Contrast-Enhanced Digital Mammography (CEDM) mammographic techniques. The important metadata were extracted from the digital mammography console. 650 subjects were clustered based on projections, age and CBT. The MGD of 2D, 3D, and CEDM was positively correlated with CBT but inversely correlated with the age factor. This study indicate MGD of CEDM was 16% and 22% lower compared to 2D and 3D techniques, respectively.

Matched MeSH terms: Mammography/methods
Effect of curing regime on the immobilization of municipal solid waste incineration fly ash in sustainable cement mortar

Liu B, Yang L, Shi J, Zhang S, Yalçınkaya Ç, Alshalif AF

Environ Pollut, 2023 Jan 15;317:120839.
PMID: 36493937 DOI: 10.1016/j.envpol.2022.120839

Stabilizing/solidificating municipal solid waste incineration fly ash (MIFA) with cement is a common strategy, and it is critical to study the high-value utilization of MIFA in ordinary Portland cement (OPC) components. With this aim, binary-binding-system mortar was produced by partially replacing OPC (∼50%) with MIFA, and the effects of different curing regimes (steam curing and carbonation curing) on the properties of the cement mortar were studied. The results showed that the setting time of the cement paste was shorten with the increase of MIFA content, and steam curing accelerated the hardening of the mixture. Although the incorporation of MIFA reduced the strength of the mortar, compared to conventional curing method, steam curing and carbonation curing increased the 3-d strength of the mortar. For high-volume MIFA mortars, the CO2-cured samples had the highest long-term strength and lowest permeability. The incorporation of MIFA increased the initial porosity of the mortar, thereby significantly increasing the carbonation degree and crystallinity of the reaction product - CaCO3. Steam curing also further narrowed the difference in the hydration degree between MIFA-modified sample and plain paste, which may be due to the enhanced hydraulic reactivity of MIFA at high temperatures. Although the incorporation of MIFA increased the porosity of the mortar, this waste-derived SCM refined the bulk pore structure and decreased the interconnected porosity. Additionally, the heavy metal leaching contents of MIFA-modified mortars were all below 1%, which meet the requirements of Chinese standards. Compared with standard curing, steam curing and carbonation curing made the early-age and long-term performance of MIFA-modified mortar better, which can promote the efficient application of MIFA in OPC products.

Matched MeSH terms: Incineration/methods
Assessment of male patients' average glandular dose during mammography procedure

Sulieman A, Salah H, Rabbaa M, Abuljoud M, Alkhorayef M, Tahir D, et al.

Appl Radiat Isot, 2023 Mar;193:110626.
PMID: 36640699 DOI: 10.1016/j.apradiso.2022.110626

Breast cancer is a common malignancy for females (25% of female cancers) and also has low incidence in males. It was estimated that 1% of all breast malignancies occur in males with mortality rate about 20%, with annual increase in incidence. Risk factors include age, family history, exposure to ionizing radiation and high estrogen and low of androgens hormones level. Diagnosis and screening are challenging due to limiting effectiveness of breast cancer screening. Therefore, patients may expose to ionizing radiation that may contribute in breast cancer incidence in males. In literature, limited studies were published regarding radiation exposure for males during mammography. The objective of this research is to quantify patient doses during male mammogram and to estimate the projected radiogenic risk during the procedure. In total, 42 male patients were undergone mammogram for breast cancer diagnosis during two consecutive years. The mean and range of patient age (years) is 45 (23-80). The mean and standard deviation (SD) of the peak tube potential and tube current time product are 28.64 ± 2. and 149 ± 35.1, respectively. The mean, and range of patients' entrance surface air kerma (ESAK, mGy) per single breast procedure was 5.3 (0.47-27.5). Male patient's received comparable radiation dose per mammogram compared to female procedures. With increasing incidence of male breast cancer, proper guidelines are necessary for the mammographic procedure are necessary to reduce unnecessary radiation doses and radiogenic risk.

Matched MeSH terms: Mammography/methods
Foodomics Reveals Anti-Obesity Properties of Cannabinoids from Hemp Oil

Ding Z, Jiang F, Shi J, Wang Y, He M, Tan CP, et al.

Mol Nutr Food Res, 2023 Jan;67(2):e2200508.
PMID: 36382382 DOI: 10.1002/mnfr.202200508

SCOPE: Molecular networking (MN) analysis intends to provide chemical insight of untargeted mass spectrometry (MS) data to the user's underlying biological questions. Foodomics is the study of chemical compounds in food using advanced omics methods. In this study, an MS-MN-based foodomics approach is developed to investigate the composition and anti-obesity activity of cannabinoids in hemp oil.
METHODS AND RESULTS: A total of 16 cannabinoids are determined in optimized microwave pretreatment of hemp oil using the developed approach. Untargeted metabolomics analysis reveals that cannabinoid extract (CE) and its major constituent (cannabidiol, CBD), can alleviate high glucose-induced increases in lipids and carbohydrates, and decreases in amino acid and nucleic acid. Moreover, CE and CBD are also found to suppress the expression levels of mdt-15, sbp-1, fat-5, fat-6, fat-7, daf-2, and elevate the expression level of daf-1, daf-7, daf-16, sod-3, gst-4, lipl-4, resulting in the decrease of lipid synthesis and the enhance of kinetism. Canonical correspondence analysis (CCA) uncovers strong associations between specific metabolic alterations and gene expression levels.
CONCLUSION: These findings from this exploratory study offer a new insight into the roles of cannabinoids in the treatment of obesity and related complications.

Matched MeSH terms: Tandem Mass Spectrometry/methods
Fulltext Detection of Colorectal Polyps from Colonoscopy Using Machine Learning: A Survey on Modern Techniques

ELKarazle K, Raman V, Then P, Chua C

Sensors (Basel), 2023 Jan 20;23(3).
PMID: 36772263 DOI: 10.3390/s23031225

Given the increased interest in utilizing artificial intelligence as an assistive tool in the medical sector, colorectal polyp detection and classification using deep learning techniques has been an active area of research in recent years. The motivation for researching this topic is that physicians miss polyps from time to time due to fatigue and lack of experience carrying out the procedure. Unidentified polyps can cause further complications and ultimately lead to colorectal cancer (CRC), one of the leading causes of cancer mortality. Although various techniques have been presented recently, several key issues, such as the lack of enough training data, white light reflection, and blur affect the performance of such methods. This paper presents a survey on recently proposed methods for detecting polyps from colonoscopy. The survey covers benchmark dataset analysis, evaluation metrics, common challenges, standard methods of building polyp detectors and a review of the latest work in the literature. We conclude this paper by providing a precise analysis of the gaps and trends discovered in the reviewed literature for future work.

Matched MeSH terms: Colonoscopy/methods
Fulltext Supervised Machine Learning Based Noninvasive Prediction of Atrial Flutter Mechanism from P-to-P Interval Variability under Imbalanced Dataset Conditions

Gul MU, Kamarul Azman MH, Kadir KA, Shah JA, Hussen S

Comput Intell Neurosci, 2023;2023:8162325.
PMID: 36909967 DOI: 10.1155/2023/8162325

Atrial flutter (AFL) is a common arrhythmia with two significant mechanisms, namely, focal (FAFL) and macroreentry (MAFL). Discrimination of the AFL mechanism through noninvasive techniques can improve radiofrequency ablation efficacy. This study aims to differentiate the AFL mechanism using a 12-lead surface electrocardiogram. P-P interval series variability is hypothesized to be different in FAFL and MAFL and may be useful for discrimination. 12-lead ECG signals were collected from 46 patients with known AFL mechanisms. Features for a proposed classifier are extracted through descriptive statistics of the interval series. On the other hand, the class ratio of MAFL and FAFL was 41 : 5, respectively, which was highly imbalanced. To resolve this, different data augmentation techniques (SMOTE, modified-SMOTE, and smoothed-bootstrap) have been applied on the interval series to generate synthetic interval series and minimize imbalance. Modification is introduced in the classic SMOTE technique (modified-SMOTE) to properly produce data samples from the original distribution. The characteristics of modified-SMOTE are found closer to the original dataset than the other two techniques based on the four validation criteria. The performance of the proposed model has been evaluated by three linear classifiers, namely, linear discriminant analysis (LDA), logistic regression (LOG), and support vector machine (SVM). Filter and wrapper methods have been used for selecting relevant features. The best average performance was achieved at 400% augmentation of the FAFL interval series (90.24% sensitivity, 49.50% specificity, and 76.88% accuracy) in the LOG classifier. The variation of consecutive P-wave intervals has been shown as an effective concept that differentiates FAFL from MAFL through the 12-lead surface ECG.

Matched MeSH terms: Electrocardiography/methods
Prevalence and implications of microplastics in potable water system: An update

Menon V, Sharma S, Gupta S, Ghosal A, Nadda AK, Jose R, et al.

Chemosphere, 2023 Mar;317:137848.
PMID: 36642147 DOI: 10.1016/j.chemosphere.2023.137848

Synthetic plastics, which are lightweight, durable, elastic, mouldable, cheap, and hydrophobic, were originally invented for human convenience. However, their non-biodegradability and continuous accumulation at an alarming rate as well as subsequent conversion into micro/nano plastic scale structures via mechanical and physio-chemical degradation pose significant threats to living beings, organisms, and the environment. Various minuscule forms of plastics detected in water, soil, and air are making their passage into living cells. High temperature and ambient humidity increase the degradation potential of plastic polymers photo-catalytically under sunlight or UV-B radiations. Microplastics (MPs) of polyethylene terephthalate, polyethylene, polystyrene, polypropylene, and polyvinyl chloride have been detected in bottled water. These microplastics are entering into the food chain cycle, causing serious harm to all living organisms. MPs entering into the food chain are usually inert in nature, possessing different sizes and shapes. Once they enter a cell or tissue, it causes mechanical damage, induces inflammation, disturbs metabolism, and even lead to necrosis. Various generation routes, types, impacts, identification, and treatment of microplastics entering the water bodies and getting associated with various pollutants are discussed in this review. It emphasizes potential detection techniques like pyrolysis, gas chromatography-mass spectrometry (GC-MS), micro-Raman spectroscopy, and fourier transform infrared spectroscopy (FT IR) spectroscopy for microplastics from water samples.

Matched MeSH terms: Environmental Monitoring/methods
Histopathologic Diagnosis Discrepancies Between Preoperative Endoscopic Forceps Biopsies and Specimens Resected by Endoscopic Submucosal Dissection in Superficial Gastric Neoplasms

Aliaga Ramos J, Pedrosa MS, Yoshida N, Abdul Rani R, Arantes VN

J Clin Gastroenterol, 2023 Jan 01;57(1):74-81.
PMID: 33883515 DOI: 10.1097/MCG.0000000000001541

BACKGROUND: The standard of practice when a superficial lesion was identified during upper GI endoscopy is to take an endoscopic forceps biopsy (EFB) of the lesion. The histopathologic findings then will determine the management plan. Endoscopic submucosal dissection (ESD) enables en-bloc resection for early neoplasms of the gastrointestinal tract and provides an adequate specimen that permits a more reliable histopathologic assessment. The objective of this study was to determine the rate of histopathologic discrepancy between EFB and specimens resected by ESD, and to identify the predisposing risk factors for this discordance.
MATERIALS AND METHODS: This is a retrospective study, enrolling patients with superficial gastric neoplasms that underwent EFB followed by ESD. We divided cases to concordant or discordant group according to the histopathologic diagnosis of EFB and ESD specimens. We also analyzed the features that may have influenced the occurrence of histopathologic discordance and the association between discordant samples of adenocarcinoma and neoplastic invasion to deeper layers.
RESULTS: A total of 115 gastric ESD procedures were performed with 84 patients meeting the inclusion criteria. Histopathologic discordance between EFB and ESD specimens were observed in 35.8% of cases (30/84 lesions). The univariant-bivariant analysis and multivariate logistic regression analysis showed that histologic discordance was closely related to the size of the lesions ( P =0.028).
CONCLUSION: Histopathologic discrepancy between EFB and ESD specimens may occur in approximately one-third of cases, particularly for lesions over 20 mm, which may lead to crucial delays in gastric cancer precise diagnosis and treatment.

Matched MeSH terms: Biopsy/methods
Fulltext Genomic selection strategies to increase genetic gain in tea breeding programs

Lubanga N, Massawe F, Mayes S, Gorjanc G, Bančič J

Plant Genome, 2023 Mar;16(1):e20282.
PMID: 36349831 DOI: 10.1002/tpg2.20282

Tea [Camellia sinensis (L.) O. Kuntze] is mainly grown in low- to middle-income countries (LMIC) and is a global commodity. Breeding programs in these countries face the challenge of increasing genetic gain because the accuracy of selecting superior genotypes is low and resources are limited. Phenotypic selection (PS) is traditionally the primary method of developing improved tea varieties and can take over 16 yr. Genomic selection (GS) can be used to improve the efficiency of tea breeding by increasing selection accuracy and shortening the generation interval and breeding cycle. Our main objective was to investigate the potential of implementing GS in tea-breeding programs to speed up genetic progress despite the low cost of PS in LMIC. We used stochastic simulations to compare three GS-breeding programs with a Pedigree and PS program. The PS program mimicked a practical commercial tea-breeding program over a 40-yr breeding period. All the GS programs achieved at least 1.65 times higher genetic gains than the PS program and 1.4 times compared with Seed-Ped program. Seed-GSc was the most cost-effective strategy of implementing GS in tea-breeding programs. It introduces GS at the seedlings stage to increase selection accuracy early in the program and reduced the generation interval to 2 yr. The Seed-Ped program outperformed PS by 1.2 times and could be implemented where it is not possible to use GS. Our results indicate that GS could be used to improve genetic gain per unit time and cost even in cost-constrained tea-breeding programs.

Matched MeSH terms: Genomics/methods
Fulltext Assessment of common errors and subjective quality of digital panoramic radiographs in dental institution, Riyadh

Lingam AS, Koppolu P, Abdulsalam R, Reddy RL, Anwarullah A, Koppolu D

Ann Afr Med, 2023;22(1):49-54.
PMID: 36695222 DOI: 10.4103/aam.aam_213_21

AIM: The study aims to determine the frequency of positioning errors, record the number of diagnostically inappropriate images, and assess the quality of panoramic radiographs.
MATERIALS AND METHODS: In this study, a total of 2629 patients for whom orthopantomogram were selected out of 3900 new patients between August 2020 and June 2021. The positioning errors of the radiographs were evaluated and categorized into ten groups. The quality of the radiographs is further assessed as "Excellent," "Diagnostically Acceptable," and "Diagnostically Unacceptable."
RESULTS: Out of the total radiographs, 32.8% had no errors, and 77.2% of the radiographs had one or more positioning errors. The radiographs were analyzed in detail, and the errors found in the panoramic radiographs were recorded. The most common positioning error observed in the radiograph was error 8, failure to place the tongue close to the palate.
CONCLUSION: The results and evaluation inferred that attention to patient positioning and focusing on reducing diagnostically indecent images could improve the quality of panoramic radiographs. Proper instructions to the patient, patient preparation, appropriate positioning of the patient, and the technician's skill plays a vital role in reducing diagnostic errors in Panoramic Radiography.

Matched MeSH terms: Radiography, Panoramic/methods
International Alliance of Urolithiasis guideline on retrograde intrarenal surgery

Zeng G, Traxer O, Zhong W, Osther P, Pearle MS, Preminger GM, et al.

BJU Int, 2023 Feb;131(2):153-164.
PMID: 35733358 DOI: 10.1111/bju.15836

OBJECTIVES: To set out the second in a series of guidelines on the treatment of urolithiasis by the International Alliance of Urolithiasis that concerns retrograde intrarenal surgery (RIRS), with the aim of providing a clinical framework for urologists performing RIRS.
MATERIALS AND METHODS: After a comprehensive search of RIRS-related literature published between 1 January 1964 and 1 October 2021 from the PubMed database, systematic review and assessment were performed to inform a series of recommendations, which were graded using modified GRADE methodology. Additionally, quality of evidence was classified using a modification of the Oxford Centre for Evidence-Based Medicine Levels of Evidence system. Finally, related comments were provided.
RESULTS: A total of 36 recommendations were developed and graded that covered the following topics: indications and contraindications; preoperative imaging; preoperative ureteric stenting; preoperative medications; peri-operative antibiotics; management of antithrombotic therapy; anaesthesia; patient positioning; equipment; lithotripsy; exit strategy; and complications.
CONCLUSION: The series of recommendations regarding RIRS, along with the related commentary and supporting documentation, offered here should help provide safe and effective performance of RIRS.

Matched MeSH terms: Ureteroscopy/methods
Comparison of LED and LASER Colonoscopy About Linked Color Imaging and Blue Laser/Light Imaging of Colorectal Tumors in a Multinational Study

Yoshida N, Draganov PV, John S, Neumann H, Rani RA, Hsu WH, et al.

Dig Dis Sci, 2023 Oct;68(10):3943-3952.
PMID: 37558800 DOI: 10.1007/s10620-023-08057-2

INTRODUCTION: In light-emitting diode (LED) and LASER colonoscopy, linked color imaging (LCI) and blue light/laser imaging (BLI) are used for lesion detection and characterization worldwide. We analyzed the difference of LCI and BLI images of colorectal lesions between LED and LASER in a multinational study.
METHODS: We prospectively observed lesions with white light imaging (WLI), LCI, and BLI using both LED and LASER colonoscopies from January 2020 to August 2021. Images were graded by 27 endoscopists from nine countries using the polyp visibility score: 4 (excellent), 3 (good), 2 (fair), and 1 (poor) and the comparison score (LED better/similar/LASER better) for WLI/LCI/BLI images of each lesion.
RESULTS: Finally, 32 lesions (polyp size: 20.0 ± 15.2 mm) including 9 serrated lesions, 13 adenomas, and 10 T1 cancers were evaluated. The polyp visibility scores of LCI/WLI for international and Japan-expert endoscopists were 3.17 ± 0.73/3.17 ± 0.79 (p = 0.92) and 3.34 ± 0.78/2.84 ± 1.22 (p

Matched MeSH terms: Colonoscopy/methods
Quantification of nimetazepam and its metabolite in human hair samples using liquid chromatography-tandem mass spectrometry: Case report

Dai Y, Han L, Wang Y, Zhao K, Gu J, Bai H, et al.

Leg Med (Tokyo), 2023 Nov;65:102303.
PMID: 37598646 DOI: 10.1016/j.legalmed.2023.102303

Nimetazepam (marketed brand names; Erimin and Lavol) is an intermediate acting benzodiazepine derivative, which was widely used mainly in East and Southeast Asian region countries including Japan, Malaysia, Brunei, the Philippines, Thailand, Indonesia, Hong Kong, Singapore and China. Nimetazepam and its metabolite 7-aminonimetazepam were quantified from human hair samples by liquid chromatography tandem-mass spectrometry (LC-MS/MS), under selective reaction monitoring mode. Using diazepam-d5 as an internal standard, the concentration of nimetazepam and its metabolite 7-aminonimetazepam could be determined by matrix matched calibration method. Extraction of the target compounds was performed by using methanol, followed by evaporation and being concentrated with nitrogen. The Limit of quantification concentrations of nimetazepam and its metabolite 7-aminonimetazepam in hair samples were both 25 pg/mg by established method. The concentrations of nimetazepam in hair samples obtained from 2 users were 27.4, and 22.0 pg/mg, respectively; the concentrations of 7-animonimetazepam in hair samples were 54.2 and 29.1 pg/mg, respectively. In our study, the 7-aminonimetazepam concentrations in hair was higher than those of nimetazepam in the authentic hair samples. To our knowledge, this is the first report to establish the detailed procedure for quantificating nimetazepam and 7-aminonimetazepam in human hair by LC-MS/MS.

Matched MeSH terms: Chromatography, Liquid/methods
Fulltext De Garengeot hernia: a rare case in an elderly woman and a review of operative approaches

Zainudin S, Hayati F, Arumugam T, Ho KY

BMJ Case Rep, 2021 Apr 16;14(4).
PMID: 33863769 DOI: 10.1136/bcr-2020-240557

De Garengeot hernia is a rare finding of the vermiform appendix inside a femoral hernia sac. We report this occurrence in a 73-year-old woman who presented in the acute setting. There are no standardised surgical approaches and many different techniques have been described in case reports in the literature. We conducted a literature review of and found a total of 113 cases with addition of our case 114 unique cases were included for analysis. Inguinal incision was most cited (n=89). Concomitant laparotomy was needed in 13 patients, however, the association between type of incision and additional laparotomy was not significant (p>0.05). Laparoscopic surgery alone was performed in eight patients. Nine patients had hybrid surgery. The most common hernia repair was through suture technique with non-absorbable material (n=31). Mesh repair was used in 28 cases. More laparoscopic surgeries were done when the disease was diagnosed preoperatively (7/39, p<0.05).

Matched MeSH terms: Laparoscopy/methods
Fulltext Massage manipulation and progression of osteosarcoma, does it really correlate: a combination of prospective and retrospective cohort study

Karda IWAM, Wan Ismail WF, Kamal AF

Sci Rep, 2023 Oct 29;13(1):18541.
PMID: 37899365 DOI: 10.1038/s41598-023-45808-7

In Indonesia, the challenge of osteosarcoma progression is further worsened by patients' dependence on traditional massage therapy, low socio-economy, and educational status. This study aims to analyze the differences in the characteristics, laboratory findings, surgery techniques, degree of histopathological necrosis, and metastasis between osteosarcoma patients with and without prior massage manipulation therapy. This research is an analytical observational study with a prospective and retrospective cohort design. Patients were treated and followed for one year to evaluate the occurrence of metastasis. Prospective data was collected through interviews, and secondary data was collected from the patient's medical record. Of 84 subjects analyzed, 69% had a history of massage. There was an increase in LDH and ALP in patients with massage manipulation (p = 0.026). The median time to metastasis from baseline in the massage group (4 months) was statistically significant compared to the non-manipulation group (12 months) (p

Matched MeSH terms: Massage/methods
Utility of public knowledge bases for the interpretation of comprehensive tumor molecular profiling results

Lebedeva A, Timokhin G, Ignatova E, Kavun A, Veselovsky E, Sharova M, et al.

Clin Exp Med, 2023 Oct;23(6):2663-2674.
PMID: 36752890 DOI: 10.1007/s10238-023-01011-6

With the growing use of comprehensive tumor molecular profiling (CTMP), the therapeutic landscape of cancer is rapidly evolving. NGS produces large amounts of genomic data requiring complex analysis and subsequent interpretation. We sought to determine the utility of publicly available knowledge bases (KB) for the interpretation of the cancer mutational profile in clinical practice. Analysis was performed across patients who previously underwent CTMP. Independent interpretation of the CTMP was performed manually, and then, the recommendations were compared to ones present in KBs (OncoKB, CIViC, CGI, CGA, VICC, MolecularMatch). A total of 222 CTMP reports from 222 patients with 932 genomic alterations (GA) were identified. For 368 targetable GA identified in 171 (77%) of the patients, 1381 therapy recommendations were compiled. Except for CGA, therapy ESCAT LOE I, II, IIIA and IIIB therapy options were equally represented in the majority of KB. Personalized treatment options with ESCAT LOE I-II were provided for 35 patients (16%); MolecularMatch/CIViC allowed to collect ESCAT I-II treatment options for 34 of them (97%), OncoKB/CGI-for 33 of them (94%). Employing VICC and CGA 6 (17%) and 20 (57%) of patients were left without ESCAT I or II treatment options. For 88 patients with ESCAT level III-B therapy recommendations: only 2 (2%), 3 (3%), 4 (5%) and 6 (7%) of patients were left without options with CIViC, MolecularMatch, CGI and OncoKB, and with VICC-12 (14%). Highest overlap ratio was observed for IIIA (0.81) biomarkers, with the comparable results for LOE I-II. Meanwhile, overlap ratio for ESCAT LOE IV was 0.22. Public KBs provide substantial information on ESCAT-I/R1 biomarkers, but the information on ESCAT II-IV and resistance biomarkers is underrepresented. Manual curation should be considered the gold standard for the CTMP interpretation.

Matched MeSH terms: Genomics/methods

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