Displaying publications 121 - 140 of 305 in total

Abstract:
Sort:
  1. Fulltext Corporate culture and health care organization
    Izamin, I., Jamsiah, M., Aniza, I.
    Malaysian Journal of Community Health, 2007;13(1):44-54.
    MyJurnal
    Each organization has a particular culture, due to personal interactions, with certain values shared by its members. Corporate culture is defined as ‘the set of shared, taken-for-granted implicit assumptions that a group holds and that determines how it perceives, thinks about, and reacts to its various environments’. Positive corporate culture is linked to increased staff alignment, advanced level of employee commitment, increased employee productivity, enhanced organizational effectiveness and increased profitability. Researchers claimed that most studies suggesting the associations between culture and performance are methodologically weak. Cultural transformation has been a big part of NHS reforms and health system redesign in United States to deliver improvements in quality and performance. Environment, market competition, technology advancement, information age and government policies will influence the cultural change within the organization. Undesirable culture might emerge if the organization does not act appropriately to manage its corporate culture. There are six critical success factors for the implementation of corporate culture changes: committed and effective leadership, clear definition of the desired goals, rigorous implementation of a change management model, effective mitigation of change resistance, active governance structure and a design model, and effective communication. Among the issues in implementing corporate culture within healthcare organizations are middle managers dilemma, cultural diversity and subcultures within the organization, size of healthcare organization and critical mass.
    Matched MeSH terms: Goals
  2. Fulltext Participation and empowerment: determining the healthcare organization’s success
    Haidar, R.T., Ahmad Fareed, A.R., Jamsiah, M.
    Malaysian Journal of Community Health, 2010;16(1):38-47.
    MyJurnal
    Introduction : An organization performs well with proper management methods. Participation is a process involving cooperation and collaboration within all levels of individuals, groups or community for the purpose of organization improvement. Empowerment brings the organization further by delegation of power and responsibilities to others for better result.
    Methodology : This is a systematic review on various publications, articles and studies based on the findings and researches conducted by experts and scholars involved with participation and empowerment of organizations.
    Results : Participation and empowerment are crucial in every organization including healthcare. Its success depends on factors such as proper planning, mutual understanding, regular training and continuous education. They contribute towards better outcome in goal setting, decision making, problem solving and organizational development. There are also various levels of empowerment and participation depending on the type of activities, organisation’s performance and level of authority.
    Conclusion : The prerequisite for an organisation’s success is dependent upon holistic participation and appropriate empowerment. The benefits gained from these measures are indeed invaluable. It creates sense of ownership, increases motivation and satisfaction, optimizing resources, promote innovations, enhancing quality and improving efficiency.
    Matched MeSH terms: Goals
  3. Fulltext Knowledge On Writing A Good Scientific Paper
    Low, W.Y.
    Malaysian Journal of Psychiatry, 2009;18(2):1-5.
    MyJurnal
    Research publication is an essential part of scientific research process. Writing is a means of communication of scientific work, a means to disseminate the research findings to the public and also to their peers. A scientific paper is a written and published report describing original research results. Getting a paper published in a well-respected peer-reviewed journal is an important goal for any researcher. It is an indication of research success. There are many reasons why people write and this ranged from pure altruism to pleasure to intellectual pursuits to contribute to the scientific knowledge, to improve patient care and to benefit the community and mankind. Reasons to write vary from one individual to another individual. Writing can be difficult and it is seen as a chore, such as, fulfilling a minimum requirement of an organization to get a job, a job confirmation or a promotion, and career development.
    Matched MeSH terms: Goals
  4. Fulltext The COMBI (Communication for Behavioural Impact ) program in the prevention and control of dengue - the Hulu Langat experienc
    Rozhan, S., Jamsiah, M., Rahimah, A., Ang, K.T.
    Malaysian Journal of Community Health, 2006;12(1):0-0.
    MyJurnal
    Background: The COMBI concept is a novel approach by the WHO to control communicable diseases which are influenced by community behaviour. The Ministry of Health is currently evaluating its use against dengue in selected areas throughout Malaysia. COMBI doctrine differs from previous dengue campaigns. It acknowledges that the factors contributing to dengue proliferation may differ between areas. Factors for a given area are analysed, then a single precise behavioural goal to overcome those problems is formulated. To inculcate this behavioural change, the target community is subjected to an intensive campaign using Integrated Marketing Communication techniques adapted from the advertisingindustry, particularly involving volunteers from the community itself.
    Methodology: In Selangor the pilot project was implemented in Section 3 and Section 4 of Bandar Baru Bangi, in the district of Hulu Langat. Here, Aedes breeding was found to occur mainly in water containers of semi permanent nature (eg. ‘kolah’, aquatic plant jars, flower pot bases etc). A total of 172 volunteers were recruited to disperse the message of “Suluh – Suluh, Basuh - Basuh” whilst distributing leaflets and flashlights to 2666 homes. Residents were instructed to illuminate such water containers twice weekly and scrub any containers found to contain larvae. The program commenced on 23/5/2004 and lasted 16 weeks.
    Results : During this period, the initial Aedes Index of 5 was reduced to 0.96 while combined cases of Dengue Fever / Dengue Haemorraghic Fever in Sections 3 and 4 reported to the Hulu Langat District Health Office also dropped to 1 (unconfirmed).
    Conclusion : The COMBI approach in Hulu Langat successfully demonstrated that correct problem identification synergized with community engagement can potentially reduce Aedes proliferationand dengue morbidity.
    Matched MeSH terms: Goals
  5. Fulltext Perception towards health promotion activities: findings from a community survey in the state of Penang, Malaysia
    Hassali, M.A., Saleem, F., Shafie, A.A., Aljadhey, H., Chua, G.N., Masood, I., et al.
    Malaysian Journal of Public Health Medicine, 2012;12(2):6-14.
    MyJurnal
    The study aimed to explore the perceptions towards health promotion activities among population of Penang Island,
    Malaysia. The study was designed as a questionnaire based cross sectional analysis. General public from the district of
    Jelutong, located in the state of Penang, Malaysia was conveniently approached for the study. Descriptive statistics
    were used to ascertain demographic characteristics where as inferential statistics were employed to measure the
    extent of association among study variables. Out of 480 respondents, a response rate of 82.7% was achieved. The study
    cohort was dominated by females (63.0%) and majority of the participants belonged to Malay ethnicity (88.1%). One
    hundred and seventy two (43.3%) never attended a health promotional campaign and mentioned lack of time and
    transport as potential barriers. Among those who attended such activities, one third was satisfied with the benefits of
    health campaigns. Approximately 90% of the participants demanded accessible locations, common language as mode of
    communication and complete medical checkups with professional advice at health promotional campaigns. General
    public can encouraged to participate in the health promotion activities by considering their priorities before designing
    a health promotion program. This will help in targeting and achieving the goal “health for all”.
    Matched MeSH terms: Goals
  6. Fulltext Comparative review of fourteen years maternal mortality in achieving MDG5 in Malaysia and UKMMC
    Abd Rahman, R., Ismail, N.M., Yassin, M.A., Sulaiman, A.S.
    Malaysian Journal of Public Health Medicine, 2013;13(1):59-63.
    MyJurnal
    Worldwide maternal mortality rate had reduced tremendously including Malaysia. At the 2000 Millenium Summit, eight Millenium Development Goals (MDGs) were established with target for the year 2015. Three years remained until the deadline to achieve MDG5, which comprised of two targets and one being reducing the maternal mortality ratio by 75%.This review compared the trend of maternal mortality and its causes in our centre to the national data. The national data had shown a reduction from 44 to 27.6 in 100,000 live births in1991 and 2008 respectively. The subsequent rate had stabilized for the past seven years. In contrast the UKM Medical Centre (UKMMC) data in the past 15 years had not been stable. The target of MDG5 seemed to be achievable by our country but may require longer time as we have yet to find ways to overcome medical care inadequacy in remote areas.
    Matched MeSH terms: Goals
  7. Factors affecting molecular self-assembly and its mechanism
    Tan, Hueyling
    Scientific Research Journal, 2012;9(1):43-61.
    MyJurnal
    Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use of peptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study of biological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries of existing disciplines. Many self-assembling systems are range from bi- and tri-block copolymers to DNA structures as well as simple and complex proteins and peptides. The ultimate goal is to harness molecular self-assembly such that design and control of bottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes of life and non-life science applications. Such aspirations can be achieved through understanding the fundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.
    Matched MeSH terms: Goals
  8. Fulltext Development of a green combat armour from Rame-Kevlar-polyester composite
    Zainab Shakir Radif, Aidy Ali, Khalina Abdan
    Pertanika Journal of Science & Technology, 2011;19(2):339-348.
    MyJurnal
    This study was conducted for the development of the green protection garments. For this purpose, laminate composite material was developed from Kevlar 29-ramie-unsaturated polyester resin. The aim of this study was to develop a solid body armour that meets the specific requirements of ballistic resistance. This composite is subjected to high impact loading. The target was shot using gas gun machine that is supported by camera hardware to capture the projectile speed. In order to achieve the goal of the research, several experiments were conducted with the aim to estimate the ballistic limit, maximum energy absorption, composite failure mode, life time rupture, target geometry, and environmental effect. The results of these experiments indicated that the maximum ballistic limit validated at impact speed is in the range of 250 m/s to 656.8 m/s for the second protection level. The targets are improved in term of the impact response with the increase in the relative humidity, i.e. the range of 50% ± 20%, whereas, reduction of resistance results in the increase of temperature. The range of temperatures was between 20oC and 70oC. A limited delamination was generated under multiple shots. Targets geometry plays a major role in increasing the impact response. Hence, the results present a high resistant impact for pairs from the panels with total thickness arrived to 15
    mm ± 3 mm. This body armour is one of the most economical armour products, in which common materials are used in its production, particularly to reduce the amount of Kevlar, and this could further lead to a decrease in its production cost. On the other hand, this armour meets the ballistic threats under 623 m/s of 15 mm ± 3 mm target thickness and 837.5 m/s of 25 mm ± 2.mm. Thus, the armour is equivalent to the third level of protective ballistic limits in the National Institute of Justice (NIJ) standards.
    Matched MeSH terms: Goals
  9. Fulltext Pulmonary rehabilitation using mechanical insufflation-exsufflation therapy for spinal cord injury – two case studies in the University Malaya Medical Centre
    Tan, J.H., Aishah A. Fauzi, Hasnan, N.
    JUMMEC, 2017;20(2):31-33.
    MyJurnal
    There is a high incidence of 36% to 83% of respiratory dysfunction in patients with acute cervical spinal cord
    injury. Complications arising from respiratory dysfunction remain one of the most common causes of morbidity
    and mortality in the spinal cord injured population. Specialized pulmonary care and therapy can help individuals
    with tetraplegia to maintain a stable respiratory status allowing active participation in active rehabilitation.
    This would allow them to achieve rehabilitation goals of independent function and community reintegration.
    Mechanical insufflation-exsufflation (MIE) therapy is an option for secretion management in individuals with
    acute spinal cord injury. In this paper, we present our experience using MIE as an adjunct to management of
    secretions in the spinal cord injured population at the University of Malaya Medical Centre.
    Matched MeSH terms: Goals
  10. Fulltext The effect of using games in teaching conservation
    Tan CKW, Lee JW, Hii A, Loo YY, Campos-Arceiz A, Macdonald DW
    PeerJ, 2018;6:e4509.
    PMID: 29736327 DOI: 10.7717/peerj.4509
    Games are an increasingly popular approach for conservation teaching. However, we know little about the effectiveness of the games on students' experiences and knowledge acquisition. Many current games are supplemental games (SG) that have no meaningful interaction with the subject matter. We adapted the experiential gaming (EG) model where students were immersed in goal-orientated tasks found in real-life situations, and they tackled questions to complete actions for their main task. Classroom-based games were created for eight different conservation topics for an annual Wildlife Conservation Course and an annual Diploma in International Wildlife Conservation Practice. Data were collected over two cycles, a total sample size of 55 multinational students. We used a combination of repeated-measures design and counterbalanced measures design; each student was subjected at least twice to each of the EG and didactic instruction (DI) treatments, and at least once to the SG approach. We compared students' perception, learning and behavioural responses to the treatments, including measures of student personality types and learning styles as explanatory variables. Findings revealed multiple benefits of the classroom EG compared to the DI approach, such as increased attention retention, increased engagement and added intrinsic motivation. The improved level of intrinsic motivation was mainly facilitated by increased social bonding between participants. Further, we show that this EG approach appeals to a wide range of learning styles and personalities. The performance of SG was generally intermediate between that of EG and DI. We propose EG as a beneficial complement to traditional classroom teaching and current gamified classes for conservation education.
    Matched MeSH terms: Goals
  11. Fulltext Sustainable development goals, universal health coverage and equity in health systems: the Orang Asli commons approach
    Wong YS, Allotey P, Reidpath DD
    Glob Health Epidemiol Genom, 2016;1:e12.
    PMID: 29868204 DOI: 10.1017/gheg.2016.8
    Universal health coverage is a key health target in the Sustainable Development Goals (SDGs) that has the means to link equitable social and economic development. As a concept firmly based on equity, it is widely accepted at international and national levels as important for populations to attain 'health for all' especially for marginalised groups. However, implementing universal coverage has been fraught with challenges and the increasing privatisation of health care provision adds to the challenge because it is being implemented in a health system that rests on a property regime that promotes inequality. This paper asks the question, 'What does an equitable health system look like?' rather than the usual 'How do you make the existing health system more equitable?' Using an ethnographic approach, the authors explored via interviews, focus group discussions and participant observation a health system that uses the commons approach such as which exists with indigenous peoples and found features that helped make the system intrinsically equitable. Based on these features, the paper proposes an alternative basis to organise universal health coverage that will better ensure equity in health systems and ultimately contribute to meeting the SDGs.
    Matched MeSH terms: Goals
  12. Fulltext Optimising drug dosing in patients receiving extracorporeal membrane oxygenation
    Cheng V, Abdul-Aziz MH, Roberts JA, Shekar K
    J Thorac Dis, 2018 Mar;10(Suppl 5):S629-S641.
    PMID: 29732181 DOI: 10.21037/jtd.2017.09.154
    Optimal pharmacological management during extracorporeal membrane oxygenation (ECMO) involves more than administering drugs to reverse underlying disease. ECMO is a complex therapy that should be administered in a goal-directed manner to achieve therapeutic endpoints that allow reversal of disease and ECMO wean, minimisation of complications (treatment of complications when they do occur), early interruption of sedation and rehabilitation, maximising patient comfort and minimising risks of delirium. ECMO can alter both the pharmacokinetics (PK) and pharmacodynamics (PD) of administered drugs and our understanding of these alterations is still evolving. Based on available data it appears that modern ECMO circuitry probably has a less significant impact on PK when compared with critical illness itself. However, these findings need further confirmation in clinical population PK studies and such studies are underway. The altered PD associated with ECMO is less understood and more research is indicated. Until robust dosing guidelines become available, clinicians will have to rely on the principles of drug dosing in critically ill and known PK alterations induced by ECMO itself. This article summarises the PK alterations and makes preliminary recommendations on possible dosing approaches.
    Matched MeSH terms: Goals
  13. Fulltext Factors influencing functional outcome of Philos Plate fixation for proximal humerus fractures
    Hazwan Ab. Wahid, Khairul Nizam bin Siron, Ahmad Zakiran
    International Medical Journal Malaysia, 2017;16(11):42-42.
    MyJurnal
    Displaced and unstable proximal humerus fractures are difficult to treat
    and they have high morbidity. The main goal is to achieve painless shoulder with full
    recovery of the shoulder joint motion. Impingement syndrome is one of the commonest
    postoperative complication. This study aim is to appreciate the functional outcomes of
    Philos-plate fixation for proximal humerus fractures and to establish association with
    high plate positioning with impingement syndrome of the shoulder after Philos-plate
    fixation. (Copied from article).
    Matched MeSH terms: Goals
  14. Fulltext Male reproductive system
    Assi, Mohammed Abdulrazzaq, Abdul Wahid Haron, Md Sabri Mohd Yusoff, Mohammed Ali Rajion, Mohd Hezmee Mohd Noor
    The Pertanika Journal of Scholarly Research Reviews, 2017;3(1):1-16.
    MyJurnal
    The overall reproductive process consists of both the human sex organs which include the male and female reproductive system. The ability to produce offsprings that have similar characteristic as their parents is the goal of reproduction. The sexual type of reproduction takes place in human and both male and female reproductive system is required. Male reproductive system is mainly concerned with production of semen (whitish viscous fluid emitted from the male reproductive tract that contains sperm and fluids) and transferring it into the female reproductive tract. In this review, we will discuss the latest findings in the research pertaining the male reproductive system and its contribution towards the research in advancement of reproductive physiology.
    Matched MeSH terms: Goals
  15. The effectiveness of seated combined extension-compression and transverse load traction in increasing cervical lordosis- challenging the underlying framework
    Tamara Gien Pooke, Rozi Mahmud, Suraini Mohamad Saini, Yap, Poh Sin, Sharifah Roohi Syed Waseem Ahmad, Harwant Singh
    International e-Journal of Science, Medicine & Education, 2016;10(3):24-33.
    MyJurnal
    Introduction: The goal of this study was to assess the effectiveness of seated combined extension-compression and transverse load (ECTL) traction as a new method for increasing a reduced lordosis of less than 30 degrees in a Malaysian population between the ages of 18 and 60 years. Possible changes in disc height were measured in accordance with the underlying theoretical framework, that suggests the anterior cervical structures would elongate due to creep over the fulcrum of the traction device.
    Method: This was a single centre, randomised, blinded controlled clinical trial with parallel groups, used to test the superiority of the seated combined ECTL traction together with physiotherapy exercises when compared with the same physiotherapy exercises used as a control. Fifty randomly allocated subjects who completed the forty treatments over the fourteen weeks were analysed using non-parametric tests for changes in outcomes.
    Results: There were no significant changes in outcomes for disc height changes seen in this study. The findings of a greater overall increase in posterior disc height changes compared with anterior disc height changes were in contrast with the proposed underlying theoretical framework for this type of ECTL traction. The greater height changes occurring in the control group were also unexpected.
    Conclusion: The findings in this study of the contrasting changes in disc height of greater posterior than anterior height changes, question the underlying theoretical framework as postulated for this type of traction.
    Matched MeSH terms: Goals
  16. Fulltext Teachers’ Pedagogical content knowledge and implementation of physical education teaching in Malaysia-Indonesia secondary schools
    Julismah Jani, Syed Kamaruzaman Syed Ali
    Jurnal Sains Sukan & Pendidikan Jasmani, 2012;1(1):1-13.
    MyJurnal
    The objectives of this study were to determine level of acquisition, implementation and differences of pedagogical content knowledge among physical education teachers It also examined the relationship between pedagogical content knowledge and its implementation during physical education lesson. About 149 of Form 4 physical education teachers in Ulu Kinta, Perak, Malaysia secondary schools and 125 physical education teachers of X class in upper secondary schools of Surabaya, Indonesia were involved in this study. Data of the study was collected through questionnaires The findings delineate a medium level (M = 2.90) of pedagogical content knowledge of Malaysian physical education teachers and 86.3% have implemented in their lessons. Whilst the Indonesian physical education teachers acquired a high level of pedagogical content knowledge (M = 3.69) and 94.2% have implemented during their physical education lessons. There was a significant (p = .00) difference among Malaysian and Indonesian physical education teachers on acquisition of pedagogical content knowledge. The findings also delineate a medium positive and significant relationship between pedagogical content knowledge and its implementation during physical education lesson of Malaysian (r = .622, p < .01) and Indonesian (r = .647, p < .01) physical education teachers respectively. The results of the study implied that pedagogical content knowledge is a specific domain to teaching process. Teachers are able to develop their current dimension and goals or contemporary of pedagogical content knowledge based on current needs and their own ability and contentment. Therefore, teachers should acquire pedagogical content knowledge so that their teaching would be meaningful and effective for students learning.
    Matched MeSH terms: Goals
  17. Fulltext Optimizing the multidimensional aspects of the patient-physician relationship in the management of inflammatory bowel disease
    Chew D, Zhiqin W, Ibrahim N, Ali RAR
    Intest Res, 2018 10;16(4):509-521.
    PMID: 30369231 DOI: 10.5217/ir.2018.00074
    The patient-physician relationship has a pivotal impact on the inflammatory bowel disease (IBD) outcomes. However, there are many challenges in the patient-physician relationship; lag time in diagnosis which results in frustration and an anchoring bias against the treating gastroenterologist, the widespread availability of medical information on the internet has resulted in patients having their own ideas of treatment, which may be incongruent from the treating physicians' goals resulting in patient physician discordance. Because IBD is an incurable disease, the goal of treatment is to sustain remission. To achieve this, patients may have to go through several lines of treatment. The period of receiving stepping up, top down or even accelerated stepping up medications may result in a lot of frustration and anxiety for the patient and may compromise the patient-physician relationship. IBD patients are also prone to psychological distress that further compromises the patient-physician relationship. Despite numerous published data regarding the medical and surgical treatment options available for IBD, there is a lack of data regarding methods to improve the therapeutic patient-physician relationship. In this review article, we aim to encapsulate the challenges faced in the patient-physician relationship and ways to overcome in for an improved outcome in IBD.
    Matched MeSH terms: Goals
  18. Feedforward backpropagation, Genetic Algorithm approaches for predicting reference evapotranspiration
    Shafika Sultan Abdullah, M.A. Malek, Namiq Sultan Abdullah, A. Mustapha
    Sains Malaysiana, 2015;44:1053-1059.
    Water scarcity is a global concern, as the demand for water is increasing tremendously and poor management of water resources will accelerates dramatically the depletion of available water. The precise prediction of evapotranspiration (ET), that consumes almost 100% of the supplied irrigation water, is one of the goals that should be adopted in order to avoid more squandering of water especially in arid and semiarid regions. The capabilities of feedforward backpropagation neural networks (FFBP) in predicting reference evapotranspiration (ET0) are evaluated in this paper in comparison with the empirical FAO Penman-Monteith (P-M) equation, later a model of FFBP+Genetic Algorithm (GA) is implemented for the same evaluation purpose. The study location is the main station in Iraq, namely Baghdad Station. Records of weather variables from the related meteorological station, including monthly mean records of maximum air temperature (Tmax), minimum air temperature (Tmin), sunshine hours (Rn), relative humidity (Rh) and wind speed (U2), from the related meteorological station are used in the prediction of ET0 values. The performance of both simulation models were evaluated using statistical coefficients such as the root of mean squared error (RMSE), mean absolute error (MAE) and coefficient of determination (R2). The results of both models are promising, however the hybrid model shows higher efficiency in predicting ET0 and could be recommended for modeling of ET0 in arid and semiarid regions.
    Matched MeSH terms: Goals
  19. Properties enhancement of TPNR-MWNTS-OMMT hybrid nanocomposites by using ultrasonic treatment
    Mou‘ad A.Tarawneh, Sahrim Hj. Ahmad, Ku Zarina K, Ibrahim N. Hassan, Yu Lih jiun, Moayad Husein Flaifel, et al.
    Sains Malaysiana, 2013;42:503-507.
    The main goal of this paper was to study the effect of ultrasonic treatment time on the mechanical properties of thermoplastic natural rubber(TPNR) reinforced with hybrid MWNTs-OMMT. The intercalation of TPNR enhancement into layers of clay by increasing the d-spacing was found using X-ray diffraction. The tensile properties of nanocomposites treated with ultrasonic increased when compared with untreated nanocomposites. The optimum ultrasonic treatment time was obtained at 3 h. The transmission electron microscope micrograph showed a combination of intercalated-exfoliated structure of the TPNR composites with organic clay and dispersion of MWNTs. The ultrasonic treatment can promote the dispersion of MWNTs-OMMT in TPNR and also improved the compatibility of hybrid filler and the TPNR matrix.
    Matched MeSH terms: Goals
  20. Fulltext Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID)
    Mad' Atari MFB, Folta KM
    BMC Res Notes, 2019 Mar 15;12(1):144.
    PMID: 30876440 DOI: 10.1186/s13104-019-4117-3
    OBJECTIVE: The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.

    RESULTS: We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.

    Matched MeSH terms: Goals
Related Terms
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links