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Conductivity comparison of Calix[8]arene-MWCNTs through spin coating technique

Faridah Lisa Supian, Darvina Lim Choo Kheng, Amira Shakila Razali

Sains Malaysiana, 2017;46:91-96.

In this study, we investigated the conductivity enhancement of calix[8]arene-multi-walled CNTs (MWCNTs) thin film.
Two types of calix[8]arenes were used, which were 5,11,17,23,29,35,41,47-p-tert-butyl-49,50,51,52,53,54,55,56-
oktakis[(carboxy)-pentoxy] -calix[8]arene (C[8]1) and 49,50,51,52,53,54,55,56 -octahydroxycalix[8]arene (C[8]2).
The monolayer properties of these two types of calix[8]arene on water subphase were examined. Later, the thin films
were fabricated by combining different ratios of each types of calix[8]arene with MWCNTs using spin coating deposition
technique. Then, the developed thin films were characterized using surface potential meter and four point probe. Thin
films of C[8]2 with hydroxyl groups at lower rims demonstrated higher surface potential and conductivity as compared
to the thin films of C[8]1 with upper rims of tert-butyl groups and lower rims of carboxyl groups. These results indicated
that the conductivity of calixarene thin films can be enhanced by MWCNTs through simple spin coating technique.

Matched MeSH terms: Nuclear Proteins
Fulltext A rare X-linked inherited mucocutaneous syndrome in two siblings

Chong LA, Ariffin H

Med J Malaysia, 2009 Dec;64(4):327-9.
PMID: 20954562 MyJurnal

We report on an 11 year-old boy with dyskeratosis congenita who presented with dystrophic nails, dysphagia, hyperpigmentation and oral leukoplakia. He had a brother who died 14 years earlier with similar presenting symptoms and aplastic anaemia. Genetic studies of our patient demonstrated the presence of a DKC1 mutation and confirmed our diagnosis. Further genetic screening revealed that his mother and one of his four sisters are heterozygous for the same mutation.

Matched MeSH terms: Nuclear Proteins/genetics
X-linked dyskeratosis congenita in Malaysia

Hamidah A, Rashid RA, Jamal R, Zhao M, Kanegane H

Pediatr Blood Cancer, 2008 Feb;50(2):432.
PMID: 17417794
Matched MeSH terms: Nuclear Proteins/genetics*
Changes in the Proteome Profile of A549 Cells Following Helichrysetin-Induced Apoptosis Suggest the Involvement of DNA Damage Response and Cell Cycle Arrest-Associated Proteins

Ho YF, Yajit NLM, Shiau JY, Malek SNA, Shyur LF, Karsani SA

Appl Biochem Biotechnol, 2023 Nov;195(11):6867-6880.
PMID: 36947367 DOI: 10.1007/s12010-023-04384-2

Our previous findings demonstrated that Helichrysetin possessed promising anti-cancer activity. It was able to induce apoptosis in the A549 cell line. However, its mechanism of action is unknown. The present study aimed to unravel possible underlying molecular mechanisms of helichrysetin-induced apoptosis in A549 (human lung carcinoma) cells using comparative quantitative proteomics (iTRAQ labeled), followed by an exhaustive bioinformatics analysis. Our results suggested that DNA damage response (DDR) and cell cycle arrest were responsible for lung cancer cell death with helichrysetin treatment. Among proteins that changed in abundance were Nrf2 and HMOX1. They are oxidative stress-related proteins and were increased in abundance. BRAT1 was also increased in abundance, suggesting an increase in DNA damage repair, indicating the occurrence of DNA damage due to oxidative stress. However, several essential DDR downstream proteins such as p-ATM, BRCA1, FANCD2, and Rb1 that would further increase DNA damage were found to be dramatically decreased in relative abundance. Cell cycle-related proteins, p53, p21, and cyclin D1, were increased while cyclin A, cyclin E, and cdk2 were decreased. This is predicted to facilitate S-phase arrest. Furthermore, excessive DNA damage and prolonged arrest would in turn result in the induction of mitochondrial-mediated apoptosis. Based on these observations, we postulate that the effects of helichrysetin were in part via the suppression of DNA damage response which led to DNA damage and prolonged cell cycle arrest. Subsequently, this event initiated mitochondrial-mediated apoptosis in A549 lung cancer cells.

Matched MeSH terms: Nuclear Proteins/pharmacology
Fulltext Analysis of Malaysia’s single stock futures and its spot price

Marzuki, R.M., Mohd, M.A., Nawawi, A.H.M., Redzwan, N.M.

Pertanika Journal of Science & Technology, 2017;25(3):735-744.
MyJurnal

Single Stock Futures (SSFs) was introduced in Bursa Malaysia on 28th April 2006. There have been many studies on derivative instruments in Malaysia; however, none is on SSFs. Various statistical methods have been used to analyse the SSFs and its spot returns, namely Descriptive Statistics, Unit Root test, VAR, Johansen and Juselius Co-integration test, Granger Causality test, Variance Decomposition test, VECM, and GARCH model. This study analyses the SSFs and spot returns of eight companies listed in Bursa Malaysia. It found that Berjaya Sports Toto Bhd and Genting Bhd have no long-run and short-run causality (Genting Bhd has bi-directional causality) while AirAsia Bhd and AMMB Holdings Bhd’s spot returns’ volatility decreased after the introduction of SSFs; it increased in the other seven companies. In addition, only AMMB Holdings Bhd futures return did not affect its spot return. Bursa Malaysia Bhd and RHB Capital Bhd spot returns lead their futures returns

Matched MeSH terms: Nuclear Proteins
Fulltext Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4

Psychiatric GWAS Consortium Bipolar Disorder Working Group

Nat Genet, 2011 Sep 18;43(10):977-83.
PMID: 21926972 DOI: 10.1038/ng.943

We conducted a combined genome-wide association study (GWAS) of 7,481 individuals with bipolar disorder (cases) and 9,250 controls as part of the Psychiatric GWAS Consortium. Our replication study tested 34 SNPs in 4,496 independent cases with bipolar disorder and 42,422 independent controls and found that 18 of 34 SNPs had P < 0.05, with 31 of 34 SNPs having signals with the same direction of effect (P = 3.8 × 10(-7)). An analysis of all 11,974 bipolar disorder cases and 51,792 controls confirmed genome-wide significant evidence of association for CACNA1C and identified a new intronic variant in ODZ4. We identified a pathway comprised of subunits of calcium channels enriched in bipolar disorder association intervals. Finally, a combined GWAS analysis of schizophrenia and bipolar disorder yielded strong association evidence for SNPs in CACNA1C and in the region of NEK4-ITIH1-ITIH3-ITIH4. Our replication results imply that increasing sample sizes in bipolar disorder will confirm many additional loci.

Matched MeSH terms: Nuclear Proteins/genetics*; Nuclear Proteins/metabolism
Clinicopathologic and immunohistochemical differences in complete and partial hydatidiform moles in a multiracial Malaysian population

Hayati AR, Tan GC

Int J Gynecol Pathol, 2005 Jul;24(3):277-85.
PMID: 15968205
Matched MeSH terms: Nuclear Proteins/biosynthesis*; Nuclear Proteins/immunology
Fulltext Observations of travelling ionospheric disturbances during storm events over Antarctica

Momani, M.A., Yatim, B., Ali, M.A.M., Abdullah, M.

ASM Science Journal, 2009;3(2):121-130.
MyJurnal

The paper examines the propagation direction and speed of large scale travelling ionospheric disturbances (LSTIDs) obtained from GPS observations of extreme geomagnetic storms during the 23rd solar cycle; these are the October 2003 and November 2003 geomagnetic storms. In the analysis, the time delay between total electron content (TEC) structures at Scott Base station (SBA) (Lat. –77.85º, Long. 166.76º), McMurdo (McM4), (Lat. –77.84º, Long. 166.95º), Davis (DAV1), (Lat. –68.58º, Long. 77.97º) and Casey station (CAS1) (Lat. –66.28º, Long. 110.52º) GPS stations as well as the distance between these stations were employed in the analysis. The measurements during the October 2003 storm showed obvious time delay between the TEC enhancement occurrences at SBA/MCM4, DAV1 and CAS1 stations. The time delay indicated a movement of the ionospheric structures from higher to lower latitudes in a velocity ranging between 0.8 km/s – 1.2 km/s. The first sudden TEC enhancement was observed at SBA/McM4 (Lat. –75.84º) followed by CAS1 station (Lat. –66.28º) and the final TEC enhancement was seen at DAV1 station (Lat. –68.58º) with TEC magnitude decreasing while moving from higher to lower latitudes. One important observation was that although the latitude of the CAS1 station was lower than the DAV1 station, the TEC enhancement was firstly seen at the CAS1 station due to the shorter distance between SBA and CAS1 compared with the distance between SBA and CAS1 of about 500 km. The TEC measurements during the November 2003 storm showed an opposite propagation direction (i.e. poleward direction from lower to higher latitudes) which was seen with a velocity ranging between 0.3 km/s – 0.4 km/s. As similar response was observed using vertical TEC measurements obtained from individual PRN satellites but with higher velocity ranges (1.2 km/s – 2.4 km/s during October
and 0.5 km/s – 0.7 km/s during November). The equatorward or poleward expansion of LSTIDs during the October and November 2003 storms was probably caused by the disturbances in the neutral temperature which occurred close to the dayside convection throat or due to the neutral wind oscillation induced by atmospheric gravity waves launched from the aurora region.

Matched MeSH terms: Nuclear Proteins
Fulltext Effects of different types and concentration of salts on the rheological and thermal properties of sin croaker and shortfin scad skin gelatin

Sarbon, N.M., Cheow, C.S., Kyaw. Z.W., Howell, N.K.

International Food Research Journal, 2014;21(1):317-324.
MyJurnal

The aims of this study were to examine the effect of salts (CaCl2, CaSO4 and MgSO4) on the rheological and thermal properties of gelatin extracted from the skins of tropical fishes, sin croaker (Johnius dussumeiri) and shortfin scad (Decapterus macrosoma). It was found that the melting temperatures of fish skin gelatins were increased by 1.5 times as compared to bovine gelatin which was only increased by 0.5 times after holding for 2 h at 5°C. The storage (G’) and loss (G”) modulus of fish skin gelatins were improved with the addition of calcium sulphate (CaSO4) and magnesium sulphate (MgSO4), respectively. However, the storage (G’) and loss (G”) modulus of gelatin solutions were decreased with the addition of calcium chloride (CaCl2). Magnesium sulphate (MgSO4) was found to be an effective salt to improve the bloom value, elastic and viscous moduli of the fish skin gelatin. This study showed that shortfin scad skin gelatin with salt addition possessed better thermal and rheological properties than sin croaker gelatin.

Matched MeSH terms: Nuclear Proteins
Fulltext Ki-67 protein as a prognostic indicator in ovarian carcinoma

Rima Melati Mat Satar, Zed Zakari Abdul Hamid, Hartini Yusuf, Maimunah Mustakim

The Pertanika Journal of Scholarly Research Reviews, 2018;4(2):71-77.
MyJurnal

Ki-67 expression is strongly correlated with tumour cell proliferation and growth. It is widely used as a proliferation marker in the routine pathological investigation. The nuclear protein Ki- 67 (pKi67) is recognised prognostic and predictive indicator for the biopsies assessment for cancer patients. Clinically, pKi67 has been revealed to associate with metastasis and the clinical stage of tumours. Furthermore, it has been presented that the expression of Ki-67 is significantly higher in malignant tissues with poorly differentiated tumour cells, as compared with normal tissue. The Ki-67 labelling index plays a vital role as an independent prognostic factor for survival rate, which includes all stages and grade categories. There is an association between the ratios of Ki-67 positive malignant cells and patient survival. This review provides an overview of recent advances in detecting Ki-67 in ovarian carcinoma.

Matched MeSH terms: Nuclear Proteins
Fulltext Buffy coat signatures of breast cancer risk in a prospective cohort study

Chung FF, Maldonado SG, Nemc A, Bouaoun L, Cahais V, Cuenin C, et al.

Clin Epigenetics, 2023 Jun 12;15(1):102.
PMID: 37309009 DOI: 10.1186/s13148-023-01509-6

BACKGROUND: Epigenetic alterations are a near-universal feature of human malignancy and have been detected in malignant cells as well as in easily accessible specimens such as blood and urine. These findings offer promising applications in cancer detection, subtyping, and treatment monitoring. However, much of the current evidence is based on findings in retrospective studies and may reflect epigenetic patterns that have already been influenced by the onset of the disease.
METHODS: Studying breast cancer, we established genome-scale DNA methylation profiles of prospectively collected buffy coat samples (n = 702) from a case-control study nested within the EPIC-Heidelberg cohort using reduced representation bisulphite sequencing (RRBS).
RESULTS: We observed cancer-specific DNA methylation events in buffy coat samples. Increased DNA methylation in genomic regions associated with SURF6 and REXO1/CTB31O20.3 was linked to the length of time to diagnosis in the prospectively collected buffy coat DNA from individuals who subsequently developed breast cancer. Using machine learning methods, we piloted a DNA methylation-based classifier that predicted case-control status in a held-out validation set with 76.5% accuracy, in some cases up to 15 years before clinical diagnosis of the disease.
CONCLUSIONS: Taken together, our findings suggest a model of gradual accumulation of cancer-associated DNA methylation patterns in peripheral blood, which may be detected long before clinical manifestation of cancer. Such changes may provide useful markers for risk stratification and, ultimately, personalized cancer prevention.

Matched MeSH terms: Nuclear Proteins
Fulltext Do cryptic species exist in Hoplobatrachus rugulosus? An examination using four nuclear genes, the cyt b gene and the complete MT genome

Yu D, Zhang J, Li P, Zheng R, Shao C

PLoS One, 2015;10(4):e0124825.
PMID: 25875761 DOI: 10.1371/journal.pone.0124825

he Chinese tiger frog Hoplobatrachus rugulosus is widely distributed in southern China, Malaysia, Myanmar, Thailand, and Vietnam. It is listed in Appendix II of CITES as the only Class II nationally-protected frog in China. The bred tiger frog known as the Thailand tiger frog, is also identified as H. rugulosus. Our analysis of the Cyt b gene showed high genetic divergence (13.8%) between wild and bred samples of tiger frog. Unexpected genetic divergence of the complete mt genome (14.0%) was also observed between wild and bred samples of tiger frog. Yet, the nuclear genes (NCX1, Rag1, Rhod, Tyr) showed little divergence between them. Despite this and their very similar morphology, the features of the mitochondrial genome including genetic divergence of other genes, different three-dimensional structures of ND5 proteins, and gene rearrangements indicate that H. rugulosus may be a cryptic species complex. Using Bayesian inference, maximum likelihood, and maximum parsimony analyses, Hoplobatrachus was resolved as a sister clade to Euphlyctis, and H. rugulosus (BT) as a sister clade to H. rugulosus (WT). We suggest that we should prevent Thailand tiger frogs (bred type) from escaping into wild environments lest they produce hybrids with Chinese tiger frogs (wild type).

Matched MeSH terms: Nuclear Proteins/genetics*
A rare case of metastatic lung carcinoma with t(15;19)(q13;p13.1) and trisomy 12

Tay Za K, Bee PC, Shanmugam H

Pathology, 2020 Feb;52(2):273-276.
PMID: 31883672 DOI: 10.1016/j.pathol.2019.10.013
Matched MeSH terms: Nuclear Proteins/genetics*
Fulltext Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins

Heim A, Grimm C, Müller U, Häußler S, Mackeen MM, Merl J, et al.

Nucleic Acids Res, 2014 Jul;42(12):7833-50.
PMID: 24914048 DOI: 10.1093/nar/gku488

The Fe(II) and 2-oxoglutarate dependent oxygenase Jmjd6 has been shown to hydroxylate lysine residues in the essential splice factor U2 auxiliary factor 65 kDa subunit (U2AF65) and to act as a modulator of alternative splicing. We describe further evidence for the role of Jmjd6 in the regulation of pre-mRNA processing including interactions of Jmjd6 with multiple arginine-serine-rich (RS)-domains of SR- and SR-related proteins including U2AF65, Luc7-like protein 3 (Luc7L3), SRSF11 and Acinus S', but not with the bona fide RS-domain of SRSF1. The identified Jmjd6 target proteins are involved in different mRNA processing steps and play roles in exon dependent alternative splicing and exon definition. Moreover, we show that Jmjd6 modifies splicing of a constitutive splice reporter, binds RNA derived from the reporter plasmid and punctually co-localises with nascent RNA. We propose that Jmjd6 exerts its splice modulatory function by interacting with specific SR-related proteins during splicing in a RNA dependent manner.

Matched MeSH terms: Nuclear Proteins/metabolism*; Nuclear Proteins/chemistry
Fulltext Pattern of hMLH1, hMSH2 and hMSH6 expression and clinical characteristics in a sample of Malaysian colorectal carcinoma cases

Khoo JJ, Gunn A, Peh SC

Malays J Pathol, 2013 Jun;35(1):45-57.
PMID: 23817394 MyJurnal

Malignant transformation from normal colonic mucosa to carcinomas may be accelerated by genetic loss or inactivation of genes of the DNA mismatch repair system. The aim of the study was to determine the local incidence and pattern of immunohistochemical expression of mismatch repair proteins namely: hMLH1, hMSH2 and hMSH6 in a series of colorectal carcinomas (CRCs) and correlate this to their clinical and pathological features. Forty-three out of 298 cases of CRCs (14.4%) showed abnormal staining pattern for mismatch repair proteins with a majority (65.1%) showing single hMLH1 loss. Tumours with mismatch repair defect (MMR-d) were frequently found at the right side of colon (p<0.001), poorly differentiated carcinomas (p<0.001), produced more mucin (p=0.007), exophytic growth (p=0.007) and were bigger (p=0.002) than tumours with no mismatch repair defect. Immunohistochemical stains for mismatch repair proteins could be done in local laboratories on these selected cases before referring for the expensive molecular test.

Matched MeSH terms: Nuclear Proteins/analysis; Nuclear Proteins/biosynthesis*
Tocotrienol-treated MCF-7 human breast cancer cells show down-regulation of API5 and up-regulation of MIG6 genes

Ramdas P, Rajihuzzaman M, Veerasenan SD, Selvaduray KR, Nesaretnam K, Radhakrishnan AK

Cancer Genomics Proteomics, 2011 Jan-Feb;8(1):19-31.
PMID: 21289334

Tocotrienols belong to the vitamin E family and have multiple anticancer effects, such as antiproliferative, antioxidant, pro-apoptosis and antimetastatic. This study aimed to identify the genes that are regulated in human breast cancer cells following exposure to various isomers of vitamin E as these may be potential targets for the treatment of breast cancer.

Matched MeSH terms: Nuclear Proteins/antagonists & inhibitors; Nuclear Proteins/genetics*
Bcp1 Is the Nuclear Chaperone of Rpl23 in Saccharomyces cerevisiae

Ting YH, Lu TJ, Johnson AW, Shie JT, Chen BR, Kumar S S, et al.

J Biol Chem, 2017 Jan 13;292(2):585-596.
PMID: 27913624 DOI: 10.1074/jbc.M116.747634

Eukaryotic ribosomes are composed of rRNAs and ribosomal proteins. Ribosomal proteins are translated in the cytoplasm and imported into the nucleus for assembly with the rRNAs. It has been shown that chaperones or karyopherins responsible for import can maintain the stability of ribosomal proteins by neutralizing unfavorable positive charges and thus facilitate their transports. Among 79 ribosomal proteins in yeast, only a few are identified with specific chaperones. Besides the classic role in maintaining protein stability, chaperones have additional roles in transport, chaperoning the assembly site, and dissociation of ribosomal proteins from karyopherins. Bcp1 has been shown to be necessary for the export of Mss4, a phosphatidylinositol 4-phosphate 5-kinase, and required for ribosome biogenesis. However, its specific function in ribosome biogenesis has not been described. Here, we show that Bcp1 dissociates Rpl23 from the karyopherins and associates with Rpl23 afterward. Loss of Bcp1 causes instability of Rpl23 and deficiency of 60S subunits. In summary, Bcp1 is a novel 60S biogenesis factor via chaperoning Rpl23 in the nucleus.

Matched MeSH terms: Nuclear Proteins/genetics; Nuclear Proteins/metabolism*
Fulltext Reduced cardiomyocyte Na+ current in the age-dependent murine Pgc-1β-/- model of ventricular arrhythmia

Ahmad S, Valli H, Smyth R, Jiang AY, Jeevaratnam K, Matthews HR, et al.

J Cell Physiol, 2019 Apr;234(4):3921-3932.
PMID: 30146680 DOI: 10.1002/jcp.27183

Peroxisome proliferator-activated receptor-γ coactivator-1 deficient (Pgc-1β-/- ) murine hearts model the increased, age-dependent, ventricular arrhythmic risks attributed to clinical conditions associated with mitochondrial energetic dysfunction. These were accompanied by compromised action potential (AP) upstroke rates and impaired conduction velocities potentially producing arrhythmic substrate. We tested a hypothesis implicating compromised Na+ current in these electrophysiological phenotypes by applying loose patch-clamp techniques in intact young and aged, wild-type (WT) and Pgc-1β-/- , ventricular cardiomyocyte preparations for the first time. This allowed conservation of their in vivo extracellular and intracellular conditions. Depolarising steps elicited typical voltage-dependent activating and inactivating inward Na+ currents with peak amplitudes increasing or decreasing with their respective activating or preceding inactivating voltage steps. Two-way analysis of variance associated Pgc-1β-/- genotype with independent reductions in maximum peak ventricular Na+ currents from -36.63 ± 2.14 (n = 20) and -35.43 ± 1.96 (n = 18; young and aged WT, respectively), to -29.06 ± 1.65 (n = 23) and -27.93 ± 1.63 (n = 20; young and aged Pgc-1β-/- , respectively) pA/μm2 (p

Matched MeSH terms: Nuclear Proteins/deficiency*; Nuclear Proteins/genetics
Expression of RING Finger Protein 38 in Serotonergic Neurons in the Brain of Nile Tilapia, Oreochromis niloticus

Cham KL, Soga T, Parhar IS

Front Neuroanat, 2018;12:109.
PMID: 30574074 DOI: 10.3389/fnana.2018.00109

Serotonin (5-hydroxytryptamine, 5-HT) is one of the major neurotransmitters, modulating diverse behaviours and physiological functions. Really interesting new gene (RING) finger protein 38 (RNF38) is an E3 ubiquitin ligase whose function remains unclear. A recent study has shown a possible regulatory relationship between RNF38 and the 5-HT system. Therefore, to gain insight into the role of RNF38 in the central 5-HT system, we identified the neuroanatomical location of 5-HT positive cells and investigated the relationship between RNF38 and the 5-HT system in the brain of the Nile tilapia, Oreochromis niloticus. Immunocytochemistry revealed three neuronal populations of 5-HT in the brain of tilapia; the paraventricular organ (PVO), the dorsal and ventral periventricular pretectal nuclei (PPd and PPv), and, the superior and inferior raphe (SR and IR). The 5-HT neuronal number was highest in the raphe (90.4 in SR, 284.6 in IR), followed by the pretectal area (22.3 in PPd, 209.8 in PPv). Double-label immunocytochemistry showed that the majority of 5-HT neurons express RNF38 nuclear proteins (66.5% in PPd; 77.9% in PPv; 35.7% in SR; 49.1% in IR). These findings suggest that RNF38 could be involved in E3 ubiquitination in the central 5-HT system.

Matched MeSH terms: Nuclear Proteins
Fulltext The rare primary Low Grade Papillary Adenocarcinoma of Nasopharynx: a diagnostic challenge and management

Noor Dina, H., Gendeh, B.S.

Medicine & Health, 2015;10(2):137-140.
MyJurnal

Low Grade Papillary Nasopharyngeal Adenocarcinoma (LGPAC) is a very rare tumour of mucosal origin compared to a higher incidence of well differentiated keratinized/non-keratinized nasopharyngeal carcinoma. It is an epithelial tumour with glandular differentiation. Its papillary figure seen histologically, is also seen in metastatic papillary thyroid carcinoma. This has caused a significant challenge to the Pathologist to differentiate primary papillary nasopharyngeal adenocarcinoma and metastatic tumour. Thyroid Transcription Factor-1 (TTF-1) is also expressed in subsets of papillary nasopharyngeal adenocarcinoma, which is valuable as a diagnostic tool. LGPAC is a benign-like malignant neoplasm. An early diagnosis with a complete tumour removal via conventional excision or endoscopic approach has offered a good prognosis with low risk of recurrence.

Matched MeSH terms: Nuclear Proteins

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