Displaying publications 1 - 20 of 184 in total

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  1. Fulltext Rapid test for Swine flu
    Wiwanitkit V
    Med J Malaysia, 2011 Mar;66(1):75.
    PMID: 23765153
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype*
  2. Detection of H5, H7 and H9 subtypes of avian influenza viruses by multiplex reverse transcription-polymerase chain reaction
    Chaharaein B, Omar AR, Aini I, Yusoff K, Hassan SS
    Microbiol Res, 2009;164(2):174-9.
    PMID: 17336046
    Subtype-specific multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to simultaneously detect three subtypes (H5, H7 and H9) of avian influenza virus (AIV) type A. The sensitivity of the multiplex RT-PCR was evaluated and compared to that of RT-PCR-enzyme-linked immunosorbent assay (ELISA) and conventional RT-PCR. While the sensitivity of the multiplex RT-PCR is as sensitive as the conventional RT-PCR, it is 10 times less sensitive than RT-PCR-ELISA. The multiplex RT-PCR is also as sensitive as the virus isolation method in detecting H9N2 from tracheal samples collected at day 3 and 5 post inoculation. Hence, the developed multiplex RT-PCR assay is a rapid, sensitive and specific assay for detecting of AIV subtypes.
    Matched MeSH terms: Influenza A virus/genetics; Influenza A virus/isolation & purification*; Influenza A Virus, H5N1 Subtype/genetics; Influenza A Virus, H5N1 Subtype/isolation & purification; Influenza A Virus, H9N2 Subtype/genetics; Influenza A Virus, H9N2 Subtype/isolation & purification
  3. Isolations of influenza A viruses from passerine birds in Malaysia
    Ibrahim HM, Awang IP, Alexander DJ, Manvell RJ, Aini I, Ibrahim AL
    Vet Rec, 1990 Nov 24;127(21):528.
    PMID: 2281590
    Matched MeSH terms: Influenza A virus/classification; Influenza A virus/isolation & purification*
  4. Fulltext Covid-19 pandemic in the lens of food safety and security
    Ma NL, Peng W, Soon CF, Noor Hassim MF, Misbah S, Rahmat Z, et al.
    Environ Res, 2021 Feb;193:110405.
    PMID: 33130165 DOI: 10.1016/j.envres.2020.110405
    The recently emerged coronavirus disease (COVID-19), which has been characterised as a pandemic by the World Health Organization (WHO), is impacting all parts of human society including agriculture, manufacturing, and tertiary sectors involving all service provision industries. This paper aims to give an overview of potential host reservoirs that could cause pandemic outbreak caused by zoonotic transmission. Amongst all, continues surveillance in slaughterhouse for possible pathogens transmission is needed to prevent next pandemic outbreak. This paper also summarizes the potential threats of pandemic to agriculture and aquaculture sector that control almost the total food supply chain and market. The history lesson from the past, emerging and reemerging infectious disease including the Severe Acute Respiratory Syndrome (SARS) in 2002, Influenza A H1N1 (swine flu) in 2009, Middle East Respiratory Syndrome (MERS) in 2012 and the recent COVID-19 should give us some clue to improve especially the governance to be more ready for next coming pandemic.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype*
  5. Fulltext Pandemic preparedness in the 21st century: which way forward?
    Khor SK, Heymann DL
    Lancet Public Health, 2021 06;6(6):e357-e358.
    PMID: 33964228 DOI: 10.1016/S2468-2667(21)00101-8
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype*
  6. Biosensing epidemic and pandemic respiratory viruses: Internet of Things with Gaussian noise channel algorithmic model
    Gopinath SCB, Ismail ZH, Sekiguchi K
    Biotechnol Appl Biochem, 2022 Dec;69(6):2507-2516.
    PMID: 34894363 DOI: 10.1002/bab.2300
    The current world condition is dire due to epidemics and pandemics as a result of novel viruses, such as influenza and the coronavirus, causing acute respiratory syndrome. To overcome these critical situations, the current research seeks to generate a common surveillance system with the assistance of a controlled Internet of Things operated under a Gaussian noise channel. To create the model system, a study with an analysis of H1N1 influenza virus determination on an interdigitated electrode (IDE) sensor was validated by current-volt measurements. The preliminary data were generated using hemagglutinin as the target against gold-conjugated aptamer/antibody as the probe, with the transmission pattern showing consistency with the Gaussian noise channel algorithm. A good fit with the algorithmic values was found, displaying a similar pattern to that output from the IDE, indicating reliability. This study can be a model for the surveillance of varied pathogens, including the emergence and reemergence of novel strains.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype*
  7. Fulltext Comparison of egg and high yielding MDCK cell-derived live attenuated influenza virus for commercial production of trivalent influenza vaccine: in vitro cell susceptibility and influenza virus replication kinetics in permissive and semi-permissive cells
    Hussain AI, Cordeiro M, Sevilla E, Liu J
    Vaccine, 2010 May 14;28(22):3848-55.
    PMID: 20307595 DOI: 10.1016/j.vaccine.2010.03.005
    Currently MedImmune manufactures cold-adapted (ca) live, attenuated influenza vaccine (LAIV) from specific-pathogen free (SPF) chicken eggs. Difficulties in production scale-up and potential exposure of chicken flocks to avian influenza viruses especially in the event of a pandemic influenza outbreak have prompted evaluation and development of alternative non-egg based influenza vaccine manufacturing technologies. As part of MedImmune's effort to develop the live attenuated influenza vaccine (LAIV) using cell culture production technologies we have investigated the use of high yielding, cloned MDCK cells as a substrate for vaccine production by assessing host range and virus replication of influenza virus produced from both SPF egg and MDCK cell production technologies. In addition to cloned MDCK cells the indicator cell lines used to evaluate the impact of producing LAIV in cells on host range and replication included two human cell lines: human lung carcinoma (A549) cells and human muco-epidermoid bronchiolar carcinoma (NCI H292) cells. The influenza viruses used to infect the indicators cell lines represented both the egg and cell culture manufacturing processes and included virus strains that composed the 2006-2007 influenza seasonal trivalent vaccine (A/New Caledonia/20/99 (H1N1), A/Wisconsin/67/05 (H3N2) and B/Malaysia/2506/04). Results from this study demonstrate remarkable similarity between influenza viruses representing the current commercial egg produced and developmental MDCK cell produced vaccine production platforms. MedImmune's high yielding cloned MDCK cells used for the cell culture based vaccine production were highly permissive to both egg and cell produced ca attenuated influenza viruses. Both the A549 and NCI H292 cells regardless of production system were less permissive to influenza A and B viruses than the MDCK cells. Irrespective of the indicator cell line used the replication properties were similar between egg and the cell produced influenza viruses. Based on these study results we conclude that the MDCK cell produced and egg produced vaccine strains are highly comparable.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype/growth & development*; Influenza A Virus, H1N1 Subtype/immunology; Influenza A Virus, H1N1 Subtype/physiology; Influenza A Virus, H3N2 Subtype/growth & development*; Influenza A Virus, H3N2 Subtype/immunology; Influenza A Virus, H3N2 Subtype/physiology
  8. Fulltext Influenza A Virus: Host-Virus Relationships
    Lal SK
    Viruses, 2020 08 09;12(8).
    PMID: 32784813 DOI: 10.3390/v12080870
    We are in the midst of a pandemic where the infective agent has been identified, but how it causes mild disease in some and fatally severe disease in other infected individuals remains a mystery [...].
    Matched MeSH terms: Influenza A virus/genetics; Influenza A virus/immunology; Influenza A virus/physiology*
  9. Fulltext Serological response to influenza vaccination among children vaccinated for multiple influenza seasons
    Rafiq S, Russell ML, Webby R, Fonseca K, Smieja M, Singh P, et al.
    PLoS One, 2012;7(12):e51498.
    PMID: 23240030 DOI: 10.1371/journal.pone.0051498
    BACKGROUND: To evaluate if, among children aged 3 to 15 years, influenza vaccination for multiple seasons affects the proportion sero-protected.

    METHODOLOGY/PRINCIPAL FINDINGS: Participants were 131 healthy children aged 3-15 years. Participants were vaccinated with trivalent inactivated seasonal influenza vaccine (TIV) over the 2005-06, 2006-07 and 2007-8 seasons. Number of seasons vaccinated were categorized as one (2007-08); two (2007-08 and 2006-07 or 2007-08 and 2005-06) or three (2005-06, 2006-07, and 2007-08). Pre- and post-vaccination sera were collected four weeks apart. Antibody titres were determined by hemagglutination inhibition (HAI) assay using antigens to A/Solomon Islands/03/06 (H1N1), A/Wisconsin/67/05 (H3N2) and B/Malaysia/2506/04. The proportions sero-protected were compared by number of seasons vaccinated using cut-points for seroprotection of 1:40 vs. 1:320. The proportions of children sero-protected against H1N1 and H3N2 was high (>85%) regardless of number of seasons vaccinated and regardless of cut-point for seroprotection. For B Malaysia there was no change in proportions sero-protected by number of seasons vaccinated; however the proportions protected were lower than for H1N1 and H3N2, and there was a lower proportion sero-protected when the higher, compared to lower, cut-point was used for sero-protection.

    CONCLUSION/SIGNIFICANCE: The proportion of children sero-protected is not affected by number of seasons vaccinated.

    Matched MeSH terms: Influenza A Virus, H1N1 Subtype/immunology; Influenza A Virus, H3N2 Subtype/immunology
  10. Influenza viruses circulating in Thailand in 2004 and 2005
    Waicharoen S, Thawatsupha P, Chittaganpitch M, Maneewong P, Thanadachakul T, Sawanpanyalert P
    Jpn J Infect Dis, 2008 Jul;61(4):321-3.
    PMID: 18653981
    Determining the local circulating strain of influenza is essential to prevent and control epidemics. In the years 2004 and 2005, the National Influenza Center of Thailand received 3,854 and 3,834 specimens, respectively, from patients throughout the country, including submissions from 4 established influenza surveillance sentinel sites. In 2004, of 539 influenza-positive specimens, 461 were positive for influenza A and 78 were positive for influenza B by isolation. Influenza A subtyping revealed that 249, 197, and 15 isolates were H1N1, H3N2, and H5N1, respectively. In 2005, of 748 influenza-positive specimens, 492 were influenza A and the remaining 256 were influenza B. The results of influenza A subtyping indicated that 55, 437, and 5 isolates were H1N1, H3N2, and H5N1. All isolated strains of subtype H1N1 were A/New Caledonia/20/99-like. The isolated strains of H3N2 were A/Fujian/411/2002-like in the first half of the year 2004, while those in the latter half of 2004 gradually drifted to a mixture of A/Wellington/1/2004-like, A/California/7/2004-like, and A/Wisconsin/67/2005-like, and this mixture continued through the end of 2005. The influenza B strains were B/Sichuan/379/99-like, B/Hong Kong/330/2001-like, B/Shanghai/361/2002-like and B/Malaysia/2506/2004-like. The strains circulating in the years 2004 and 2005 were antigenically similar to the vaccine formulas recommended in the same period by WHO. Our results underscore that local influenza surveillance plays an important role in responding to epidemics and potential pandemics.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype/isolation & purification; Influenza A Virus, H3N2 Subtype/isolation & purification
  11. Site-directed fragment-based generation of virtual sialic acid databases against influenza A hemagglutinin
    Al-qattan MN, Mordi MN
    J Mol Model, 2010 May;16(5):975-91.
    PMID: 19856192 DOI: 10.1007/s00894-009-0606-y
    In this study fragment-based drug design is combined with molecular docking simulation technique, to design databases of virtual sialic acid (SA) analogues with new substitutions at C2, C5 and C6 positions of SA scaffold. Using spaces occupied by C2, C5 and C6 natural moieties of SA when bound to hemagglutinin (HA) crystallographic structure, new fragments that are commercially available were docked independently in all the pockets. The oriented fragments were then connected to the SA scaffold with or without incorporation of linker molecules. The completed analogues were docked to the whole SA binding site to estimate their binding conformations and affinities, generating three databases of HA-bound SA analogues. Selected new analogues showed higher estimated affinities than the natural SA when tested against H3N2, H5N1 and H1N1 subtypes of influenza A. An improvement in the binding energies indicates that fragment-based drug design when combined with molecular docking simulation is capable to produce virtual analogues that can become lead compound candidates for anti-flu drug discovery program.
    Matched MeSH terms: Influenza A Virus, H5N1 Subtype/immunology; Influenza A Virus, H5N1 Subtype/metabolism*; Influenza A Virus, H5N1 Subtype/chemistry
  12. [Development of new nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and assessment of growth characteristics of influenza virus vaccine strains cultivated on them]
    Mazurkova NA, Troshkova, Shishkina LN, Stavskiĭ EA, Drozdov IG
    Zh. Mikrobiol. Epidemiol. Immunobiol., 2011 3 31.
    PMID: 21449080
    AIM: To develop nutrient medium for MDCK and Vero cells based on soy hydrolysate obtained using bromeline and to assess of growth characteristics of influenza virus vaccine strains cultivated on them.

    MATERIALS AND METHODS: Physico-chemical characteristics of hydrolysate were assessed according to FS 42-3874-99. Growth characteristics of nutrient medium based on soy hydrolysate and vaccine strains of influenza virus A/Solomon Islands/03/06 (H1N1), A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004 were studied on MDCK and Vero cells.

    RESULTS: MDCK and Vero cells grew well on medium based on soy hydrolysate obtained using bromeline with decreased (to 2% and 3% respectively) content of fetal calf serum and allowed effective production of vaccine strains of influenza virus.

    CONCLUSION: Technology for producing of nutrient medium based on hydrolysate of soy flour obtained using bromeline was developed. This medium could successively used for cultivation of continued cell cultures MDCK and Vero used as substrate for tissue culture-based vaccines against influenza.

    Matched MeSH terms: Influenza A Virus, H1N1 Subtype/growth & development*; Influenza A Virus, H3N2 Subtype/growth & development*
  13. Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice
    Joan SS, Pui-Fong J, Song AA, Chang LY, Yusoff K, AbuBakar S, et al.
    Biotechnol Lett, 2016 May;38(5):793-9.
    PMID: 26758876 DOI: 10.1007/s10529-016-2034-2
    An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype
  14. Intercontinental circulation of human influenza A(H1N2) reassortant viruses during the 2001-2002 influenza season
    Xu X, Smith CB, Mungall BA, Lindstrom SE, Hall HE, Subbarao K, et al.
    J Infect Dis, 2002 Nov 15;186(10):1490-3.
    PMID: 12404167
    Reassortant influenza A viruses bearing the H1 subtype of hemagglutinin (HA) and the N2 subtype of neuraminidase (NA) were isolated from humans in the United States, Canada, Singapore, Malaysia, India, Oman, Egypt, and several countries in Europe during the 2001-2002 influenza season. The HAs of these H1N2 viruses were similar to that of the A/New Caledonia/20/99(H1N1) vaccine strain both antigenically and genetically, and the NAs were antigenically and genetically related to those of recent human H3N2 reference strains, such as A/Moscow/10/99(H3N2). All 6 internal genes of the H1N2 reassortants examined originated from an H3N2 virus. This article documents the first widespread circulation of H1N2 reassortants on 4 continents. The current influenza vaccine is expected to provide good protection against H1N2 viruses, because it contains the A/New Caledonia/20/99(H1N1) and A/Moscow/10/99(H3N2)-like viruses, which have H1 and N2 antigens that are similar to those of recent H1N2 viruses.
    Matched MeSH terms: Influenza A virus/classification; Influenza A virus/genetics; Influenza A virus/physiology*; Influenza A virus/chemistry
  15. Fulltext Edible bird's nest modulate intracellular molecular pathways of influenza A virus infected cells
    Haghani A, Mehrbod P, Safi N, Kadir FA, Omar AR, Ideris A
    BMC Complement Altern Med, 2017 Jan 05;17(1):22.
    PMID: 28056926 DOI: 10.1186/s12906-016-1498-x
    Edible Bird's Nest (EBN) as a popular traditional Chinese medicine is believed to have health enhancing and antiviral activities against influenza A virus (IAV); however, the molecular mechanism behind therapeutic effects of EBN is not well characterized.
    Matched MeSH terms: Influenza A virus/drug effects*; Influenza A virus/physiology; Influenza A Virus, H1N1 Subtype/drug effects*; Influenza A Virus, H1N1 Subtype/physiology
  16. [The 2005-2006 influenza season in the Netherlands and the vaccine composition for the 2006-2007 season]
    Rimmelzwaan GF, de Jong JC, Donker GA, Meijer A, Fouchier RA, Osterhaus AD
    Ned Tijdschr Geneeskd, 2006 Oct 7;150(40):2209-14.
    PMID: 17061434
    The first sign of influenza activity in the Netherlands during the 2005-2006 influenza season was the isolation of influenza viruses in the last week of 2005. From Week 1 of 2006 onwards, an increase in clinical influenza activity was also observed that did not return to baseline levels until Week 15. Two waves of influenza activity were observed with peak incidences of 13.8 and 9.8 influenza-like illnesses per 10,000 inhabitants on Weeks 7 and 12, respectively. The first wave of influenza was caused primarily by influenza B viruses, whereas the second wave was caused predominantly by influenza A/H3N2 viruses. The influenza B viruses appeared to belong to two different phylogenetic lineages and were antigenically distinguishable from the vaccine strain. The isolated influenza A/H3N2 viruses were closely related to the vaccine strain for this subtype and only minor antigenic differences with the vaccine strain were observed for a limited number of isolates. Only a small number of influenza A/H1N1 viruses were isolated, which all closely resembled the H1N1 vaccine strain. For the 2006-2007 influenza season, the World Health Organization has recommended the following vaccine composition: A/Wisconsin/67/05 (H3N2), A/New Caledonia/20/99 (H1N1) and B/Malaysia/2506/05.
    Matched MeSH terms: Influenza A Virus, H1N1 Subtype/immunology*; Influenza A Virus, H1N1 Subtype/isolation & purification; Influenza A Virus, H3N2 Subtype/immunology*; Influenza A Virus, H3N2 Subtype/isolation & purification
  17. Anti-influenza virus activity of tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone
    Yazawa K, Kurokawa M, Obuchi M, Li Y, Yamada R, Sadanari H, et al.
    Antivir Chem Chemother, 2011;22(1):1-11.
    PMID: 21860068 DOI: 10.3851/IMP1782
    We examined the anti-influenza virus activity of tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, against five viruses: A/Solomon islands/3/2006 (H1N1), A/Hiroshima/52/2005 (H3N2), A/California/07/2009 (H1N1pdm), A/Narita/1/2009 (H1N1pdm) and B/Malaysia/2506/2004 strains in vitro and against A/PR/8/34 virus in vivo.
    Matched MeSH terms: Influenza A virus/drug effects*; Influenza A Virus, H1N1 Subtype/drug effects; Influenza A Virus, H3N2 Subtype/drug effects
  18. Fulltext Host Lipid Rafts Play a Major Role in Binding and Endocytosis of Influenza A Virus
    Verma DK, Gupta D, Lal SK
    Viruses, 2018 11 18;10(11).
    PMID: 30453689 DOI: 10.3390/v10110650
    Influenza still remains one of the most challenging diseases, posing a significant threat to public health. Host lipid rafts play a critical role in influenza A virus (IAV) assembly and budding, however, their role in polyvalent IAV host binding and endocytosis had remained elusive until now. In the present study, we observed co-localization of IAV with a lipid raft marker ganglioside, GM1, on the host surface. Further, we isolated the lipid raft micro-domains from IAV infected cells and detected IAV protein in the raft fraction. Finally, raft disruption using Methyl-β-Cyclodextrin revealed significant reduction in IAV host binding, suggesting utilization of host rafts for polyvalent binding on the host cell surface. In addition to this, cyclodextrin mediated inhibition of raft-dependent endocytosis showed significantly reduced IAV internalization. Interestingly, exposure of cells to cyclodextrin two hours post-IAV binding showed no such reduction in IAV entry, indicating use of raft-dependent endocytosis for host entry. In summary, this study demonstrates that host lipid rafts are selected by IAV as a host attachment factors for multivalent binding, and IAV utilizes these micro-domains to exploit raft-dependent endocytosis for host internalization, a virus entry route previously unknown for IAV.
    Matched MeSH terms: Influenza A virus/physiology*
  19. Fulltext Influenza A Viral Infection with Septic Shock in Pregnancy
    Lwin S, San Yi M, Shi Leong M, Suharjono H, Moe Nwe T
    Case Rep Obstet Gynecol, 2019;2019:2470352.
    PMID: 31139480 DOI: 10.1155/2019/2470352
    The influenza virus is RNA virus and is classified into four subtypes, influenza A, influenza B, influenza C, and influenza D. One of the subtypes of influenza A, the H1N1 strain, also known as swine flu, is especially of high risk for development of complications in pregnant women. The influenza A virus infection is difficult to diagnose clinically because its presenting symptoms are similar to those of the common cold but are more severe, last longer, and can be potentially life-threatening. This case also presented with common cold symptoms but her condition worsened later. Fortunately, obstetric health providers were vigilant enough to address the developing infection and its related complications. It was the cooperative effort of multidisciplinary team care which resulted in a favourable outcome in both mother and baby.
    Matched MeSH terms: Influenza A virus; Influenza A Virus, H1N1 Subtype
  20. Fulltext Clinical and laboratory analysis of influenza B infection in children in Taichung, Taiwan during the 2006-2007 flu season
    Wang CC, Chen PY, Wang JD, Liu FC, Huang FL, Lee CY
    Pediatr Neonatol, 2009 Apr;50(2):54-8.
    PMID: 19453079 DOI: 10.1016/S1875-9572(09)60033-4
    An epidemic of influenza B/Malaysia/2506/2004 was reported in Taiwan during the 2006-2007 flu season. We investigated the flu vaccination history and clinical and Laboratory characteristics of children with influenza B infection.
    Matched MeSH terms: Influenza A virus/isolation & purification
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