Browse publications by year: 2012

  1. Mohamed R, Ng CJ, Tong WT, Abidin SZ, Wong LP, Low WY
    BMC Public Health, 2012;12:601.
    PMID: 22856889 DOI: 10.1186/1471-2458-12-601
    BACKGROUND: Hepatitis B (HBV) is the leading cause of cirrhosis and hepatocellular carcinoma worldwide. This study assessed the knowledge, attitudes and practices of people with chronic HBV and the associated factors.
    METHODS: This cross-sectional study was conducted at an outpatient adult hepatology clinic at a tertiary hospital in Kuala Lumpur. A self-administered questionnaire was administered on a one-to-one basis to assess knowledge, attitudes, and lifestyle practices of people with chronic HBV.
    RESULTS: The response rate was 89% (n = 483/543). Participants had a mean age of 46.3 (±14.7) years and the mean duration of HBV from time of diagnosis was 12.2 (±8.8) years. The mean knowledge score was 12.57/20 (standard deviation: ±4.4, range: 0-19). Participants aged 30-39 years, with higher educational attainment, employed in professional jobs, longer duration of diagnosis and those without cirrhosis had significantly higher knowledge scores. Age, education level and duration of diagnosis were significant predictors of the knowledge score on standard multiple regression analysis. More than half of the participants were worried of spreading HBV infection to family and friends and worried since the diagnosis. A third of the participants (33.5%) were embarrassed to reveal their diagnosis to the public but most of them (93.6%) would inform their family. Those who reported feeling worried since their diagnosis were more likely to be middle-aged, of Malay ethnicity, have shorter duration of diagnosis of less than 10 years and have received therapy. About half of the participants (50.6%) did not share dining utensils and the majority (93.2%) believed that HBV can be transmitted by sharing of eating and drinking utensils. Older patients were significantly less likely to share utensils. Those who felt worried since diagnosis had significant higher knowledge of HBV.
    CONCLUSION: The findings highlight the stigma and misconceptions that still exist among the HBV patients. More patient and public education about HBV and its prevention are essential to increase awareness and to demystify the disease.
    Study site: Hepatology clinic, University of Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia
    MeSH terms: Adult; Cross-Sectional Studies; Female; Hospitals, University; Humans; Health Knowledge, Attitudes, Practice*; Life Style*; Malaysia; Male; Middle Aged; Outpatient Clinics, Hospital; Surveys and Questionnaires; Hepatitis B, Chronic/psychology*; Hepatitis B, Chronic/therapy*; Tertiary Care Centers
  2. Wong YM, Brigham CJ, Rha C, Sinskey AJ, Sudesh K
    Bioresour Technol, 2012 Oct;121:320-7.
    PMID: 22858502 DOI: 10.1016/j.biortech.2012.07.015
    The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conversion of P(3HB-co-3HHx) copolymer. Crude palm kernel oil (CPKO) at a concentration of 2.5 g/L was found to be most suitable for production of copolymer with a 3HHx content of approximately 70 mol%. The time profile of these cells was also examined in order to study the trend of 3HHx monomer incorporation, PHA production and PHA synthase activity. (1)H NMR and (13)C NMR analyses confirmed the presence of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction, in which monomers were not randomly distributed. The results of various characterization analyses revealed that the copolymers containing a high 3HHx monomer fraction demonstrated soft and flexible mechanical properties.
    MeSH terms: Biotechnology/methods*; Caproates/analysis*; Chromatography, Gas; Models, Biological; Magnetic Resonance Spectroscopy; Plant Oils/chemistry*; Time Factors; Cupriavidus necator/genetics; Cupriavidus necator/metabolism*; Polyhydroxyalkanoates/biosynthesis*; Polyhydroxyalkanoates/chemistry
  3. Kavitha N, Noordin R, Kit-Lam C, Sasidharan S
    Molecules, 2012 Aug 02;17(8):9207-19.
    PMID: 22858841 DOI: 10.3390/molecules17089207
    The inhibitory effect of active fractions of Eurycoma longifolia (E. longifolia) root, namely TAF355 and TAF401, were evaluated against Toxoplasma gondii (T. gondii). In our previous study, we demonstrated that T. gondii was susceptible to TAF355 and TAF401 with IC₅₀ values of 1.125 µg/mL and 1.375 µg/mL, respectively. Transmission (TEM) and scanning electron microscopy (SEM) observations were used to study the in situ antiparasitic activity at the IC₅₀ value. Clindamycin was used as positive control. SEM examination revealed cell wall alterations with formation of invaginations followed by completely collapsed cells compared to the normal T. gondii cells in response to the fractions. The main abnormality noted via TEM study was decreased cytoplasmic volume, leaving a state of structural disorganization within the cell cytoplasm and destruction of its organelles as early as 12 h of treatment, which indicated of rapid antiparasitic activity of the E. longifolia fractions. The significant antiparasitic activity shown by the TAF355 and TAF401 active fractions of E. longifolia suggests their potential as new anti-T. gondii agent candidates.
    MeSH terms: Animals; Antiprotozoal Agents/pharmacology*; Cercopithecus aethiops; Host-Parasite Interactions/drug effects; Microscopy, Electron, Scanning; Plant Extracts/pharmacology*; Toxoplasma/drug effects*; Toxoplasma/ultrastructure; Vero Cells; Plant Roots/chemistry*; Inhibitory Concentration 50; Eurycoma/chemistry*; Microscopy, Electron, Transmission
  4. Khajotia R, Raman S
    Can Fam Physician, 2012 Jul;58(7):757-60.
    PMID: 22859639
    MeSH terms: Chylothorax/complications; Chylothorax/etiology*; Cystic Fibrosis/diagnosis; Diagnosis, Differential; Emphysema/diagnosis; Histiocytosis, Langerhans-Cell/diagnosis; Humans; Lung Neoplasms/complications; Lung Neoplasms/diagnosis*; Male; Pneumothorax/complications; Pneumothorax/etiology*; Lymphangioleiomyomatosis/complications; Lymphangioleiomyomatosis/diagnosis*; Young Adult
  5. Su AT, Darus A, Bulgiba A, Maeda S, Miyashita K
    J Occup Health, 2012;54(5):349-60.
    PMID: 22863899
    The internationally accepted limit values and the health effects of hand-transmitted vibration exposure have been described extensively in the literature from temperate climate countries but not from a tropical climate environment.

    OBJECTIVES: We conducted a systematic review of the health effects of hand-transmitted vibration exposure in tropical countries to determine the characteristics of hand-arm vibration syndrome in a warm environment and compared the findings with the results of the systematic reviews published by the US NIOSH.

    METHODS: We searched major medical databases including MEDLINE, PubMed, Embase, CINAHL, Ovid and Cochrane based on the terms "hand arm vibration syndrome," "hand transmitted vibration," "vibration white finger" and "Raynaud" up to January 2011. Only studies conducted in a tropical or subtropical environment were selected for the review. The quality of the selected papers was assessed independently by two investigators using predefined criteria. A standard set of information was abstracted from the papers for review.

    RESULTS: Only six papers from tropical countries and three papers from subtropical countries were available in the literature. No vibration white finger was reported in the tropical countries. Neurological symptoms were prevalent in the vibration-exposed workers. Finger coldness seems to be an important surrogate for vascular disorder in a tropical environment. Meta-analysis could not be performed due to inadequacy of the information reported in these papers.

    CONCLUSIONS: The current dose-response relationship in ISO5349-1 for hand-transmitted vibration exposure is not applicable to a tropical environment. Further studies on hand-arm vibration syndromes in tropical countries are needed.

    MeSH terms: Humans; Tropical Climate*; Hand-Arm Vibration Syndrome/physiopathology*
  6. Thevi T, Mimiwati Z, Reddy SC
    Nepal J Ophthalmol, 2012;4(2):263-70.
    PMID: 22864032 DOI: http://dx.doi.org/10.3126/nepjoph.v4i2.6542
    To determine the factors affecting the visual outcome in patients with open globe injuries of eye.
    MeSH terms: Adolescent; Adult; Aged; Child; Female; Follow-Up Studies; Humans; Male; Middle Aged; Prognosis; Prospective Studies; Ophthalmologic Surgical Procedures/methods*; Tomography, X-Ray Computed; Visual Acuity/physiology*; Trauma Severity Indices; Eye Injuries, Penetrating/diagnosis; Eye Injuries, Penetrating/physiopathology*; Eye Injuries, Penetrating/surgery; Young Adult
  7. Hwu WL, Okuyama T, But WM, Estrada S, Gu X, Hui J, et al.
    Mol Genet Metab, 2012 Sep;107(1-2):136-44.
    PMID: 22864057 DOI: 10.1016/j.ymgme.2012.07.019
    Mucopolysaccharidosis (MPS) type VI (Maroteaux-Lamy syndrome) is a clinically heterogeneous lysosomal storage disorder. It presents significant diagnostic and treatment challenges due to the rarity of the disease and complexity of the phenotype. As information about MPS VI in Asia-Pacific countries is limited, a survey was conducted to assess current practices for diagnosis and management of MPS VI in this region. The participants were selected based on their experience in diagnosing and managing MPS patients.
    MeSH terms: Asia; Australia; Humans; Mucopolysaccharidosis VI/diagnosis*; Mucopolysaccharidosis VI/therapy*; Physicians; Surveys and Questionnaires; Health Care Surveys
  8. Carran M, Shaw IC
    N Z Med J, 2012;125(1358):52-63.
    PMID: 22864157
    It is well known that the endocrine-disrupting chemical (EDC) dibutylphthalate (DBP) inhibits testosterone synthesis and can lead to feminisation in male laboratory animals. Moreover, it has long been speculated that human exposure would result in the similar effects, but this is difficult to study because specific human exposure cohorts are rare. We report increases in the incidences of hypospadias (p<0.05), cryptorchidism (p<0.05) and breast cancer (p<0.05) in the children of New Zealand soldiers who served in Malaya (1948-1960) and were exposed to DBP applied daily to their clothing as an acaricide to prevent tick-transmitted bush typhus. In addition, we modelled absorption of DBP from the soldiers' clothing and using published data for skin absorption, and calculated a large theoretical absorbed dose of 64 mg/kg body weight/day which is similar to DBP's lowest observed adverse effect level (LOAEL) of 50 mg/kg body weight/day and thus indicates a biological effect is possible. This is the first report of a multigenerational developmental effect following DBP exposure in human males.
    MeSH terms: Adult; Breast Neoplasms/etiology*; Breast Neoplasms/epidemiology; Clothing; Cryptorchidism/etiology*; Cryptorchidism/epidemiology; Dibutyl Phthalate/adverse effects*; Dibutyl Phthalate/chemistry; Female; Genital Diseases, Female/etiology; Genital Diseases, Female/epidemiology; Genital Diseases, Male/etiology; Genital Diseases, Male/epidemiology; Humans; Hypospadias/etiology*; Hypospadias/epidemiology; Malaysia; Male; New Zealand/epidemiology; Plasticizers/adverse effects*; Plasticizers/chemistry; Puberty, Precocious/etiology; Puberty, Precocious/epidemiology; Retrospective Studies; Skin Absorption; Veterans*; Incidence; Paternal Exposure/adverse effects*; Acaricides/adverse effects*; Acaricides/chemistry
  9. Hashim H, Mughrabi FF, Ameen M, Khaledi H, Ali HM
    Molecules, 2012 Aug 03;17(8):9306-20.
    PMID: 22864239 DOI: 10.3390/molecules17089306
    Indolic compounds have attracted a lot of attention due to their interesting biological properties. The present study was performed to evaluate the subacute toxicity and anti-ulcer activity of BClHC against ethanol-induced gastric ulcers. Experimental animal groups were orally pre-treated with different doses of BClHC (50, 100, 200 and 400 mg/kg) in 10% Tween 20 solution (vehicle). Blank and ulcer control groups were pre-treated with vehicle. The positive group was orally pretreated with 20 mg/kg omeprazole. After one hour, all groups received absolute ethanol (5 mL/kg) to generate gastric mucosal injury except the blank control group which was administered the vehicle solution. After an additional hour, all rats were sacrificed, and the ulcer areas of the gastric walls determined. Grossly, the ulcer control group exhibited severe mucosal injury, whereas pre-treatment with either derivative or omeprazole resulted in significant protection of gastric mucosal injury. Flattening of gastric mucosal folds was also observed in rats pretreated with BClHC. Histological studies of the gastric wall of ulcer control group revealed severe damage of gastric mucosa, along with edema and leucocytes infiltration of the submucosal layer compared to rats pre-treated with either BClHC or omeprazole where there were marked gastric protection along with reduction or absence of edema and leucocytes infiltration of the submucosal layer. Subacute toxicity study with a higher dose of derivative (5 g/kg) did not manifest any toxicological signs in rats. In conclusions, the present finding suggests that benzyl N'-(5-chloroindol-3-ylmethylidene)hydrazinecarbodithioate promotes ulcer protection as ascertained by the comparative decreases in ulcer areas, reduction of edema and leucocytes infiltration of the submucosal layer.
    MeSH terms: Ethanol; Animals; Anti-Ulcer Agents/pharmacology*; Anti-Ulcer Agents/therapeutic use; Benzyl Compounds/pharmacology*; Benzyl Compounds/therapeutic use; Drug Evaluation, Preclinical; Female; Gastric Mucosa/drug effects*; Gastric Mucosa/metabolism; Gastric Mucosa/pathology; Glycoproteins/metabolism; Hydrazines/pharmacology*; Hydrazines/therapeutic use; Kidney/drug effects; Kidney/pathology; Kidney/physiopathology; Liver/drug effects; Liver/pathology; Liver/physiology; Male; Omeprazole/pharmacology; Omeprazole/therapeutic use; Stomach Ulcer/chemically induced; Stomach Ulcer/prevention & control*; Rats, Wistar; Cytoprotection; Rats
  10. Wahab MS, Nyfort-Hansen K, Kowalski SR
    Int J Clin Pharm, 2012 Dec;34(6):855-62.
    PMID: 22864867 DOI: 10.1007/s11096-012-9681-8
    BACKGROUND: The elderly population is increasing worldwide. Due to age-related physiological changes that affect the pharmacokinetics and pharmacodynamics of drugs, the elderly are predisposed to adverse drug reactions. Prescribing of potentially inappropriate medications (PIMs) has been found to be prevalent among the elderly and PIM use has been associated with hospitalisations and mortality.

    OBJECTIVES: This study aims to identify the prevalence and nature of pre-admission inappropriate prescribing by using the STOPP (screening tool of older people's prescriptions) criteria amongst a sample of hospitalised elderly inpatients in South Australia.

    SETTING: Medical, surgical and rehabilitation wards of a public teaching hospital in Adelaide, South Australia.

    MAIN OUTCOME MEASURE: Pre-admission prevalence of PIM.

    METHOD: Medication management plans of 100 patients of ≥65 years old were prospectively studied to determine the prevalence of pre-admission PIM use. Sixty-five criteria of STOPP were applied to identify PIMs.

    RESULTS: The total number of pre-admission medications screened during the study period was 949; the median number of medicines per patient was nine (range 2-28). Overall the STOPP criteria identified 138 PIMs in 60 % of patients. The most frequently encountered PIM was opiates prescribed in patients with recurrent falls (12.3 %), followed by benzodiazepines in fallers (10.1 %) and proton pump inhibitors when prescribed for peptic ulcer disease for long-term at maximum doses (9.4 %). The number of medications were found to have a positive correlation with pre-admission PIM use (r(s) = 0.49, P < 0.01).

    CONCLUSIONS: Pre-admission PIM use is highly prevalent among the studied population. Strategies to reduce PIM use should be undertaken by physicians and pharmacists. The use of the STOPP criteria in clinical practice to reduce prescriptions of inappropriate medications requires further investigation.

    MeSH terms: Age Factors; Aged; Aged, 80 and over; Cross-Sectional Studies; Drug Interactions; Female; Health Services for the Aged*; Hospitals, Public; Hospitals, Teaching; Humans; Inpatients*; Male; Drug Prescriptions; Prospective Studies; South Australia; Polypharmacy; Medication Reconciliation*
  11. Mohd Zain Z, Ab Ghani S, O'Neill RD
    Amino Acids, 2012 Nov;43(5):1887-94.
    PMID: 22865247 DOI: 10.1007/s00726-012-1365-0
    This paper discusses the application of a reagentless, selective microbiosensor as a useful alternative tool for monitoring D-serine in neural samples. The main components of the 125-μm-diameter disk biosensor were D-amino acid oxidase for D-serine sensitivity (linear region slope, 61 ± 7 μA cm(-2) mM(-1); limit of detection, 20 nM), and poly-phenylenediamine for rejection of electroactive interference. The response time of the biosensor was of the order of 1 s, ideal for 'real-time' monitoring, and detection of systemically administered D-serine in brain extracellular fluid is demonstrated. Exploitation of this probe might resolve queries involving regulation of D-serine in excitotoxicity, and modulation of N-methyl-D-aspartate receptor function by D-serine and glycine in the central nervous system.
    MeSH terms: Animals; Brain/drug effects*; Brain/physiology; Brain Chemistry/drug effects*; D-Amino-Acid Oxidase/metabolism; D-Amino-Acid Oxidase/chemistry; Electrodes, Implanted; Glycine/pharmacology; Kinetics; Neurons/drug effects*; Neurons/metabolism; Phenylenediamines/chemistry; Serine/metabolism*; Serine/pharmacology; Stereoisomerism; Biosensing Techniques*; Receptors, N-Methyl-D-Aspartate/metabolism; Extracellular Fluid/drug effects*; Extracellular Fluid/chemistry; Rats; Limit of Detection
  12. Qi Qi C, Ajit Singh V
    BMJ Case Rep, 2012;2012.
    PMID: 22865804 DOI: 10.1136/bcr-2012-006401
    Marjolin's ulcers are malignancies that arise from previously traumatised, chronically inflamed or scarred skin. We present a case with childhood burns, who had repeated irritation of his forearm skin with palm oil thorns that eventually led to malignant change.
    MeSH terms: Activities of Daily Living; Agriculture; Burns/complications*; Burns/surgery; Carcinoma, Squamous Cell/diagnosis*; Carcinoma, Squamous Cell/etiology*; Carcinoma, Squamous Cell/surgery; Cell Transformation, Neoplastic*; Cicatrix/complications*; Cicatrix/etiology; Forearm Injuries/complications*; Humans; Male; Middle Aged; Pain, Intractable; Plant Oils; Skin Neoplasms/diagnosis*; Skin Neoplasms/etiology; Skin Neoplasms/surgery; Skin Ulcer/etiology; Wound Healing; Wounds, Penetrating; Weight Loss; Treatment Outcome; Reconstructive Surgical Procedures
  13. Muhamad H, Zainol M, Sahid I, Abu Seman I
    Drug Test Anal, 2012 Aug;4 Suppl 1:112-7.
    PMID: 22851367 DOI: 10.1002/dta.1351
    In oil palm plantations, the fungicide hexaconazole is used to control Ganoderma infection that threatens to destroy or compromisethe palm. The application of hexaconazole is usually through soil drenching, trunk injection, or a combination of these two methods. It is therefore important to have a method to determine the residual amount of hexaconazole in the field such as in samples of water, soil, and leaf to monitor the use and fate of the fungicide in oil palm plantations. This study on the behaviour of hexaconazole in oil palm agro-environment was carried out at the UKM-MPOB Research Station, Bangi Lama, Selangor. Three experimental plots in this estate with 7-year-old Dura x Pisifera (DxP) palms were selected for the field trial. One plot was sprayed with hexaconazole at the manufacturer's recommended dosage, one at double the recommended dosage, and the third plot was untreated control. Hexaconazole residues in the soil, leaf, and water were determined before and after fungicide treatment. Soil samples were randomly collected from three locations at different depths (0-50 cm) and soil collected fromthe same depth were bulked together. Soil, water, and palm leaf were collected at -1 (day before treatment), 0 (day of treatment), 1, 3, 7, 14, 21, 70, 90, and 120 days after treatment. Hexaconazole was detected in soil and oil palm leaf, but was not detected in water from the nearby stream.
    MeSH terms: Environmental Monitoring; Fresh Water/analysis*; Fungicides, Industrial/analysis*; Fungicides, Industrial/isolation & purification; Soil Pollutants/analysis; Soil Pollutants/isolation & purification; Triazoles/analysis*; Triazoles/isolation & purification; Water Pollutants, Chemical/analysis; Water Pollutants, Chemical/isolation & purification; Plant Leaves/chemistry*; Arecaceae/chemistry*; Solid Phase Extraction
  14. Kon TW, Bong CF, King JH, Leong CT
    Pak J Biol Sci, 2012 Feb 01;15(3):108-20.
    PMID: 22866541
    Termites are the major decomposers in tropical region but yet their occurrences in oil palm plantation especially in peat soil are generally treated as pest. Study of termite species in peat land was conducted in selected oil palm plantations in North Sarawak with 5-7 years old palms and South Sarawak with 13-15 years old palms with two sites in each area. Results of quadrate (25 x 25 x 30 cm) sampling showed termite was significantly higher in relative density with increasing depth of soil (0-10 = 21.23, 10-20 = 42.52 and 20-30 cm = 81.12%) which could be advantaged from being predated by ants (Hymenoptera: Formicidae) which were higher in density from soil surface to 10 cm soil depth with relative density of 31.84%. Modified transect sampling (50x6 m) had successfully sampled 18 species of termites from 2 families (Rhinotermitidae and Termitidae), 5 subfamilies (Rhinotermitinae, Coptotermitinae, Termitinae, Macrotermitinae and Nasutitermitinae) and 11 genera (Coptotermes, Schedorhinotermes, Termes, Macrotermes, Nasutitermes, Globitermes, Amitermes, Parrhinotermes, Pericapritermes, Havilanditermes and Prohamitermes). Both plantation sites have termite dominantly feeding on rotten wood as a result of abundant dead woods. However, Coptotermes curvignathus Holmgren was identified to feed on the living tissues of oil palm causing damage or death of the tree. Study showed higher encounter of soil-feeding termite in longer established plantation. It indicates the gradually shifting of soil condition towards a stabilized environment which favors the successful settlement of soil feeder termite species. Termite control should be more targets specific to avoid harming beneficial termites.
    MeSH terms: Animals; Behavior, Animal; Environment; Malaysia; Pesticides; Plant Oils/chemistry; Soil; Species Specificity; Trees; Isoptera/genetics*; Isoptera/physiology*; Arecaceae/metabolism*; Biodiversity*
  15. Wey MC, Shim CN, Lee MY, Jamaluddin M, Ngeow WC
    Aust Orthod J, 2012 May;28(1):17-21.
    PMID: 22866589
    This study aimed to establish a safety zone for the placement of mini-implants in the buccal surface between the second maxillary premolar (PM2) and first maxillary molar (M1) of Mongoloids.
    MeSH terms: Alveolar Process/anatomy & histology; Alveolar Process/surgery; Bicuspid; Dental Arch/anatomy & histology*; Humans; Maxilla; Molar; Odontometry; Radiography, Panoramic; Dental Implants; Tooth Apex/anatomy & histology; Asian Continental Ancestry Group*; Orthodontic Anchorage Procedures/instrumentation*
  16. Al-Khatib AR, Rajion ZA, Masudi SM, Hassan R, Townsend GC
    Aust Orthod J, 2012 May;28(1):22-9.
    PMID: 22866590
    The development of three-dimensional computer imaging has many applications in dentistry, including the analysis of dental casts.
    MeSH terms: Dental Arch/anatomy & histology*; Dental Models; Humans; Image Processing, Computer-Assisted; Odontometry/methods*; Photogrammetry/instrumentation*; Reproducibility of Results; Observer Variation; Tooth Crown/anatomy & histology*; Imaging, Three-Dimensional/instrumentation; Imaging, Three-Dimensional/methods*
  17. A Hamid MS, Mohamed Ali MR, Yusof A, George J
    BMC Musculoskelet Disord, 2012 Aug 06;13:138.
    PMID: 22866670 DOI: 10.1186/1471-2474-13-138
    BACKGROUND: Muscle injuries are one of the commonest injuries affecting athletes. It often leads to significant pain and disability causing loss of training and competition time. With current treatment, the duration to return-to-play ranges form six weeks to never, depending on injury severity. Recent researches have suggested that autologous platelet-rich plasma (PRP) injection into the injured site may hasten soft tissues healing. To-date, there has been no randomised clinical trials to evaluate the effects of PRP on muscle healing. The aim of this study is to examine the effects of autologous PRP on duration to return-to-play after muscle injury.

    METHODS AND DESIGN: A randomised, single blind controlled trial will be conducted. Twenty-eight patients aged 18 years and above with a recent grade-2 hamstring injury will be invited to take part. Participants will be randomised to receive either autologous PRP injection with rehabilitation programme, or rehabilitation programme only. Participants will be followed up at day three of study and then weekly for 16 weeks. At each follow up visit, participants will be assessed on readiness to return-to-play using a set of criteria. The primary end-point is when participants have fulfilled the return-to-play criteria or end of 16 weeks.The main outcome measure of this study is the duration to return-to-play after injury.

    CONCLUSION: This study protocol proposes a rigorous and potential significant evaluation of PRP use for grade-2 hamstring injury. If proven effective such findings could be of great benefit for patients with similar injuries.

    TRIAL REGISTRATION: Current Controlled Trials ISCRTN66528592.

    MeSH terms: Athletic Injuries/diagnosis; Athletic Injuries/physiopathology; Athletic Injuries/rehabilitation*; Biomechanical Phenomena; Combined Modality Therapy; Humans; Injections, Intramuscular; Leg Injuries/diagnosis; Leg Injuries/physiopathology; Leg Injuries/rehabilitation*; Malaysia; Research Design*; Severity of Illness Index; Time Factors; Single-Blind Method; Range of Motion, Articular; Treatment Outcome; Ultrasonography, Interventional; Muscle, Skeletal/injuries*; Muscle, Skeletal/physiopathology; Recovery of Function; Physical Therapy Modalities*; Muscle Strength; Platelet-Rich Plasma*
  18. ul Haq N, Hassali MA, Shafie AA, Saleem F, Aljadhey H
    Health Qual Life Outcomes, 2012 Aug 06;10:91.
    PMID: 22866752 DOI: 10.1186/1477-7525-10-91
    OBJECTIVE: The study aims to assess Health Related Quality of Life (HRQoL) among Hepatitis B (HB) patients and to identify significant predictors of the HRQoL in HB patients of Quetta, Pakistan.

    METHODS: A cross sectional study by adopting European Quality of Life scale (EQ-5D) for the assessment of HRQoL was conducted. All registered HB patients attending two public hospitals in Quetta, Pakistan were approached for study. Descriptive statistics were used to describe demographic and disease related characteristics of the patients. HRQoL was scored using values adapted from the United Kingdom general population survey. EQ-5D scale scores were compared with Mann-Whitney and Kruskal-Wallis test. Standard multiple regression analysis was performed to identify predictors of HRQoL. All analyses were performed using SPSS v 16.0.

    RESULTS: Three hundred and ninety HB patients were enrolled in the study. Majority of the participants (n = 126, 32.3%) were categorized in the age group of 18-27 years (36.07 ± 9.23). HRQoL was measured as poor in the current study patients (0.3498 ± 0.31785). The multivariate analysis revealed a significant model (F(10, 380) = 40.04, P 

    MeSH terms: Adolescent; Adult; Age Factors; Cross-Sectional Studies; Female; Health Status Indicators*; Hepatitis B/epidemiology; Hepatitis B/psychology*; Hospitals, Public; Humans; Male; Middle Aged; Pakistan/epidemiology; Psychometrics; Quality of Life*; Surveys and Questionnaires; Regression Analysis; Severity of Illness Index; Sex Factors; Social Class; Sickness Impact Profile
  19. Lim KL, Jazayeri SD, Yeap SK, Alitheen NB, Bejo MH, Ideris A, et al.
    BMC Vet Res, 2012;8:132.
    PMID: 22866758 DOI: 10.1186/1746-6148-8-132
    DNA vaccines offer several advantages over conventional vaccines in the development of effective vaccines against avian influenza virus (AIV). However, one of the limitations of the DNA vaccine in poultry is that it induces poor immune responses. In this study, chicken interleukin (IL) -15 and IL-18 were used as genetic adjuvants to improve the immune responses induced from the H5 DNA vaccination in chickens. The immunogenicity of the recombinant plasmid DNA was analyzed based on the antibody production, T cell responses and cytokine production, following inoculation in 1-day-old (Trial 1) and 14-day-old (Trial 2) specific-pathogen-free chickens. Hence, the purpose of the present study was to explore the role of chicken IL-15 and IL-18 as adjuvants following the vaccination of chickens with the H5 DNA vaccine.
    MeSH terms: Animals; Antibodies, Viral/blood; Cercopithecus aethiops; Chickens; DNA, Viral/genetics; DNA, Viral/immunology*; Influenza in Birds/immunology; Influenza in Birds/prevention & control*; Hemagglutination Inhibition Tests; Influenza Vaccines/immunology; Interleukin-1/immunology*; Plasmids/genetics*; Vero Cells; T-Lymphocyte Subsets; Hemagglutinin Glycoproteins, Influenza Virus/immunology*; Vaccines, DNA/immunology; Reverse Transcriptase Polymerase Chain Reaction/veterinary; Interleukin-18/immunology*
  20. Al-Sheraji SH, Ismail A, Manap MY, Mustafa S, Yusof RM, Hassan FA
    Food Chem, 2012 Nov 15;135(2):356-61.
    PMID: 22868099 DOI: 10.1016/j.foodchem.2012.04.120
    The effect of a yoghurt supplement containing Bifidobacterium pseudocatenulatum G4 or Bifidobacterium longum BB536 on plasma lipids, lipid peroxidation and the faecal excretion of bile acids was examined in rats fed a cholesterol-enriched diet. After 8 weeks, the rats in the positive control (PC) group who were fed the cholesterol-enriched diet showed significant increases in plasma total cholesterol (TC), low-density lipoprotein (LDL) cholesterol, and malondialdehyde (MDA). However, groups fed a cholesterol-enriched diet supplemented with yoghurt containing B. pseudocatenulatum G4 or B. longum BB536 had significantly lower plasma TC, LDL-C, very-low-density lipoprotein (VLDL) cholesterol, and MDA than had the PC group after 8 weeks of treatment. In addition, faecal excretion of bile acids was markedly increased in the rats fed the yoghurt containing B. pseudocatenulatum G4 or B. longum BB536 as compared to the PC and NC groups.
    MeSH terms: Animals; Anticholesteremic Agents/metabolism*; Bifidobacterium/metabolism*; Cholesterol/blood; Cholesterol/metabolism; Humans; Hypercholesterolemia/diet therapy*; Hypercholesterolemia/metabolism; Hypercholesterolemia/microbiology; Male; Yogurt/analysis; Yogurt/microbiology*; Rats, Sprague-Dawley; Probiotics/metabolism; Probiotics/therapeutic use*; Rats
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