Browse publications by year: 2012

Fulltext Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent

Gumel AM, Annuar MS, Heidelberg T

PLoS One, 2012;7(9):e45214.
PMID: 23028854 DOI: 10.1371/journal.pone.0045214

The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. (± 0.2) (o)C, glass transition temperature (T(g)) of -1.0 (± 0.2) (o)C and apparent melting enthalpy of fusion (ΔH(f)) of 100.9 (± 0.1) J g(-1).

MeSH terms: Carbon/metabolism; Fatty Acids/analysis*; Gas Chromatography-Mass Spectrometry; Molecular Conformation; Molecular Weight; Magnetic Resonance Spectroscopy; Plant Oils/chemistry*; Temperature; Thermodynamics; Pseudomonas putida/metabolism*; Arecaceae/microbiology; Phase Transition; Polyhydroxyalkanoates/biosynthesis*; Polyhydroxyalkanoates/isolation & purification*
Fulltext IGF2BP2 alternative variants associated with glutamic acid decarboxylase antibodies negative diabetes in Malaysian subjects

Salem SD, Saif-Ali R, Ismail IS, Al-Hamodi Z, Poh R, Muniandy S

PLoS One, 2012;7(9):e45573.
PMID: 23029108 DOI: 10.1371/journal.pone.0045573

The association of Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) common variants (rs4402960 and rs1470579) with type 2 diabetes (T2D) has been performed in different populations. The aim of this study was to evaluate the association of alternative variants of IGF2BP2; rs6777038, rs16860234 and rs7651090 with glutamic acid decarboxylase antibodies (GADA) negative diabetes in Malaysian Subjects.

MeSH terms: Adult; Aged; Alleles; Diabetes Mellitus, Type 2/genetics*; Diabetes Mellitus, Type 2/immunology; Female; Gene Frequency; Genotype; Humans; Malaysia; Male; Middle Aged; Linkage Disequilibrium; RNA-Binding Proteins/genetics*; Polymorphism, Single Nucleotide*; Asian Continental Ancestry Group/genetics*
Fulltext Does the axillary lymph node ratio have any added prognostic value over pN staging for South East Asian breast cancer patients?

Saxena N, Hartman M, Yip CH, Bhoo-Pathy N, Khin LW, Taib NA, et al.

PLoS One, 2012;7(9):e45809.
PMID: 23029254 DOI: 10.1371/journal.pone.0045809

Lymph node ratio (LNR, i.e. the ratio of the number of positive nodes to the total number of nodes excised) is reported to be superior to the absolute number of nodes involved (pN stage) in classifying patients at high versus low risk of death following breast cancer. The added prognostic value of LNR over pN in addition to other prognostic factors has never been assessed.

MeSH terms: Adult; Aged; Aged, 80 and over; Axilla; Breast Neoplasms/metabolism; Breast Neoplasms/mortality; Breast Neoplasms/pathology*; Female; Humans; Lymph Nodes/pathology*; Lymphatic Metastasis; Middle Aged; Prognosis; Receptors, Estrogen/metabolism; Receptors, Progesterone/metabolism; Risk; ROC Curve; Singapore; Multivariate Analysis; Proportional Hazards Models; Area Under Curve; Kaplan-Meier Estimate; Young Adult
Fulltext Ethnic differences in the prevalence of metabolic syndrome: results from a multi-ethnic population-based survey in Malaysia

Rampal S, Mahadeva S, Guallar E, Bulgiba A, Mohamed R, Rahmat R, et al.

PLoS One, 2012;7(9):e46365.
PMID: 23029497 DOI: 10.1371/journal.pone.0046365

The prevalence of metabolic syndrome is increasing disproportionately among the different ethnicities in Asia compared to the rest of the world. This study aims to determine the differences in the prevalence of metabolic syndrome across ethnicities in Malaysia, a multi-ethnic country.

MeSH terms: Adolescent; Adult; Age Factors; Aged; Blood Glucose/metabolism; Blood Pressure; Cholesterol/blood; Cross-Sectional Studies; Ethnic Groups*; Female; Humans; Cholesterol, HDL; Cholesterol, LDL/blood; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Body Mass Index; Prevalence; Metabolic Syndrome X/blood; Metabolic Syndrome X/ethnology*; Metabolic Syndrome X/epidemiology*; Metabolic Syndrome X/physiopathology; Young Adult; Obesity, Abdominal/blood; Obesity, Abdominal/ethnology*; Obesity, Abdominal/epidemiology*; Obesity, Abdominal/physiopathology
Adsorption, desorption and mobility of metsulfuron-methyl in soils of the oil palm agroecosystem in Malaysia

Ismail BS, Ooi KE

J Environ Biol, 2012 May;33(3):573-7.
PMID: 23029905

Laboratory experiments were conducted to evaluate adsorption, desorption and mobility of metsulfuron-methyl in soils of the oil palm agroecosystem consisting of the Bernam, Selangor, Rengam and Bongor soil series. The lowest adsorption of metsulfuron-methyl occurred in the Bongor soil (0.366 ml g(-1)), and the highest in the Bemam soil (2.837 ml g(-1). The K(fads) (Freundlich) values of metsulfuron-methyl were 0.366, 0.560, 1.570 and 2.837 ml g(-1) in Bongor, Rengam, Selangor and Bemam soil, respectively. The highest K(fdes) value of metsulfuron-methyl, observed in the Bemam soil, was 2.563 indicating low desorption 0.280 (relatively strong retention). In contrast, the lowest K(fdes) value of 0.564 was observed for the Bongor soil, which had the lowest organic matter (1.43%) and clay content (13.2%). Soil organic matter and clay content were the main factors affecting the adsorption of metsulfuron-methyl. The results of the soil column leaching studies suggested that metsulfuron-methyl has a moderate potential for mobility in the Bernam and Bongor soil series with 19.3% and 39%, respectively for rainfall at 200 mm. However, since metsulfuron-methyl is applied at a very low rate (the maximum field application rate used was 30 g ha(-1)) and is susceptible to biodegradation, the potential forground water contamination is low.

MeSH terms: Adsorption; Agriculture; Arylsulfonates/analysis*; Herbicides/analysis*; Malaysia; Pesticide Residues/analysis*; Arecaceae*
Abstracts of the 14th Congress of the International Society for Peritoneal Dialysis. Kuala Lumpur, Malaysia. September 9-12, 2012

Perit Dial Int, 2012 Sep;32 Suppl 3:S89-188.
PMID: 23030011
MeSH terms: Humans; Peritoneal Dialysis*
Statistical optimization of green fluorescent protein production from Escherichia coli BL21(DE3)

Chew FN, Tan WS, Boo HC, Tey BT

Prep Biochem Biotechnol, 2012;42(6):535-50.
PMID: 23030465 DOI: 10.1080/10826068.2012.660903

An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. Three variables (agitation rate, temperature, and time of induction) exerted significant effects on functional GFP production in E. coli shake flask cultivation and were optimized subsequently using the Box-Behnken design. An agitation rate of 206 rpm at 31°C and induction of the protein expression when the cell density (OD(600nm)) reaches 1.04 could enhance the yield of functional GFP production from 0.025 g/L to 0.241 g/L, which is about ninefold higher than the unoptimized conditions. Unoptimized cultivation conditions resulted in protein aggregation and hence reduced the quantity of functional GFP. The model and regression equation based on the shake flask cultivation could be applied to a 2-L bioreactor for maximum functional GFP production.

MeSH terms: Culture Media/chemistry; Electrophoresis, Polyacrylamide Gel; Escherichia coli/growth & development; Escherichia coli/chemistry*; Fermentation; Genetic Vectors/chemistry; Hydrogen-Ion Concentration; Plasmids/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/standards; Recombinant Proteins/chemistry; Temperature; Time Factors; Models, Statistical; Cell Culture Techniques/methods; Cell Culture Techniques/standards; Bioreactors/standards; Green Fluorescent Proteins/isolation & purification*; Green Fluorescent Proteins/standards*; Green Fluorescent Proteins/chemistry; Bacterial Load
Fulltext Peripheral PDLIM5 expression in bipolar disorder and the effect of olanzapine administration

Zain MA, Jahan SN, Reynolds GP, Zainal NZ, Kanagasundram S, Mohamed Z

BMC Med Genet, 2012;13:91.
PMID: 23031404 DOI: 10.1186/1471-2350-13-91

One of the genes suggested to play an important role in the pathophysiology of bipolar disorder (BPD) is PDLIM5, which encodes LIM domain protein. Our main objective was to examine the effect of olanzapine treatment on PDLIM5 mRNA expression in the peripheral blood leukocytes of BPD patients.

MeSH terms: Adult; Benzodiazepines/therapeutic use*; Bipolar Disorder/blood; Bipolar Disorder/drug therapy*; Bipolar Disorder/genetics*; Female; Genetic Markers; Humans; Leukocytes/drug effects; Leukocytes/metabolism; Male; Middle Aged; RNA, Messenger/blood; RNA, Messenger/genetics; Antipsychotic Agents/therapeutic use*; Gene Expression/drug effects; Case-Control Studies; Polymorphism, Single Nucleotide; Adaptor Proteins, Signal Transducing/genetics*; LIM Domain Proteins/genetics*
Fulltext Use of multiple data sources to estimate the economic cost of dengue illness in Malaysia

Shepard DS, Undurraga EA, Lees RS, Halasa Y, Lum LCS, Ng CW

Am J Trop Med Hyg, 2012 Nov;87(5):796-805.
PMID: 23033404 DOI: 10.4269/ajtmh.2012.12-0019

Dengue represents a substantial burden in many tropical and sub-tropical regions of the world. We estimated the economic burden of dengue illness in Malaysia. Information about economic burden is needed for setting health policy priorities, but accurate estimation is difficult because of incomplete data. We overcame this limitation by merging multiple data sources to refine our estimates, including an extensive literature review, discussion with experts, review of data from health and surveillance systems, and implementation of a Delphi process. Because Malaysia has a passive surveillance system, the number of dengue cases is under-reported. Using an adjusted estimate of total dengue cases, we estimated an economic burden of dengue illness of US$56 million (Malaysian Ringgit MYR196 million) per year, which is approximately US$2.03 (Malaysian Ringgit 7.14) per capita. The overall economic burden of dengue would be even higher if we included costs associated with dengue prevention and control, dengue surveillance, and long-term sequelae of dengue.

MeSH terms: Dengue/economics; Dengue/epidemiology*; Humans; Malaysia/epidemiology; Cost of Illness*
Fulltext Cerebral venous sinus thrombosis presenting with diplopia in pregnancy: a case report

Munira Y, Sakinah Z, Zunaina E

J Med Case Rep, 2012;6:336.
PMID: 23034080 DOI: 10.1186/1752-1947-6-336
Fulltext Discrepancy in patient-rated and oncologist-rated performance status on depression and anxiety in cancer: a prospective study protocol

Mei Hsien CC, Wan Azman WA, Md Yusof M, Ho GF, Krupat E

BMJ Open, 2012;2(5).
PMID: 23035016 DOI: 10.1136/bmjopen-2012-001799

Psychological distress is common in patients with cancer. We need a rapid means of screening for and identifying depression and anxiety in patients with cancer. The present study evaluates the potential of the Eastern Cooperative Oncology Group (ECOG) performance status (PS) scoring as a brief screening tool to assess psychological distress in routine cancer care. The ECOG PS is widely used by oncologists and the WHO, as a standardised measure to assess general well-being in patients with cancer and quality of life in cancer trials. We examine the discrepancy between patient-rated and oncologist-rated PS scores on the ECOG in a comparative assessment against the Hospital Anxiety and Depression Scale (HADS).

MeSH terms: Anxiety; Anxiety Disorders; Depression; Humans; Neoplasms; Quality of Life; Early Detection of Cancer; Oncologists
Fulltext Hexaplex PCR detection system for identification of five human Plasmodium species with an internal control

Chew CH, Lim YA, Lee PC, Mahmud R, Chua KH

J Clin Microbiol, 2012 Dec;50(12):4012-9.
PMID: 23035191 DOI: 10.1128/JCM.06454-11

Malaria remains one of the major killers of humankind and persists to threaten the lives of more than one-third of the world's population. Given that human malaria can now be caused by five species of Plasmodium, i.e., Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, and the recently included Plasmodium knowlesi, there is a critical need not only to augment global health efforts in malaria control but also, more importantly, to develop a rapid, accurate, species-sensitive/species-specific, and economically effective diagnostic method for malaria caused by these five species. Therefore, in the present study, a straightforward single-step hexaplex PCR system targeting five human Plasmodium 18S small-subunit rRNAs (ssu rRNAs) was designed, and the system successfully detected all five human malaria parasites. In addition, this system enables the differentiation of single infection as well as mixed infections up to the two-species level. This assay was validated with 50 randomly blinded test and 184 clinical samples suspected to indicate malaria. This hexaplex PCR system is not only an ideal alternative for routine malaria diagnosis in laboratories with conventional PCR machines but also adds value to diagnoses when there is a lack of an experienced microscopist or/and when the parasite morphology is confusing. Indeed, this system will definitely enhance the accuracy and accelerate the speed in the diagnosis of malaria, as well as improve the efficacy of malaria treatment and control, in addition to providing reliable data from epidemiological surveillance studies.

MeSH terms: DNA, Ribosomal/genetics; Humans; Malaria/diagnosis*; Malaria/parasitology*; Parasitology/methods*; Parasitology/standards; Plasmodium/classification*; Plasmodium/genetics; Plasmodium/isolation & purification*; Reference Standards; RNA, Ribosomal, 18S/genetics; DNA, Protozoan/genetics; Molecular Diagnostic Techniques/methods*; Molecular Diagnostic Techniques/standards; Multiplex Polymerase Chain Reaction/methods*; Multiplex Polymerase Chain Reaction/standards
Nanowire dimensions effect on ON/OFF current ratio and sub-threshold slope in silicon nanowire transistors

Hashim Y, Sidek O

J Nanosci Nanotechnol, 2012 Sep;12(9):7101-4.
PMID: 23035439

This paper shows the effect of the dimensions of nanowires on threshold voltage, ON/OFF current ratio, and sub-threshold slope. These parameters are critical factors of the characteristics of silicon nanowire transistors. The MuGFET simulation tool was used to investigate the characteristics of a transistor. Current-voltage characteristics with different dimensions were simulated. Results show that long nanowires with low diameter and oxide thickness tend to have the best transistor characteristics.

MeSH terms: Organic Chemicals; Oxides; Silicon; Transistors, Electronic; Nanowires; Physical Phenomena
Motorcycle-related spinal injury: crash characteristics

Zulkipli ZH, Abdul Rahmat AM, Mohd Faudzi SA, Paiman NF, Wong SV, Hassan A

Accid Anal Prev, 2012 Nov;49:237-44.
PMID: 23036400 DOI: 10.1016/j.aap.2011.12.011

This study presents an analysis of crash characteristics of motorcyclists who sustained spinal injuries in motorcycle crashes. The aim of the study is to identify the salient crash characteristics that would help explain spinal injury risks for motorcyclists. Data were retrospectively collected from police case reports that were archived at MIROS from year 2005 to 2007. The data were categorized into two subcategories; the first group was motorcycle crashes with spinal injury (case) and the second group was motorcycle crashes without spinal injury (control). A total of 363 motorcyclists with spinal injury and 873 motorcyclists without spinal injury were identified and analyzed. Descriptive analysis and multivariate analysis were performed in order to determine the odds of each characteristic in contributing to spinal injury. Single vehicle crash, collision with fixed objects and crash configuration were found to have significant influence on motorcyclists in sustaining spinal injury (p<0.05). Although relatively few than other impact configurations, the rear-end impacted motorcyclist shows the highest risk of spinal injury. Helmets have helped to reduce head injury but they did not seem to offer corresponding protection for the spine in the study. With a growing number of young motorcyclists, further efforts are needed to find effective measures to help reduce the crash incidents and severity of spinal injury. In sum, the study provides some insights on some vital crash characteristics associated with spinal injury that can be further investigated to determine the appropriate counter-measures and prevention strategies to reduce spinal injury.

MeSH terms: Accidents, Traffic/mortality; Accidents, Traffic/statistics & numerical data*; Adolescent; Adult; Aged; Aged, 80 and over; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Motorcycles*; Retrospective Studies; Risk Factors; Spinal Cord Injuries/etiology*; Spinal Cord Injuries/mortality; Spinal Injuries/etiology*; Spinal Injuries/mortality; Multivariate Analysis; Logistic Models; Odds Ratio; Case-Control Studies; Databases, Factual; Young Adult
Fulltext Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

Mohamed EA, Siddiqui MJ, Ang LF, Sadikun A, Chan SH, Tan SC, et al.

BMC Complement Altern Med, 2012;12:176.
PMID: 23039079 DOI: 10.1186/1472-6882-12-176

In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities.

MeSH terms: alpha-Amylases/antagonists & inhibitors*; Diabetes Mellitus, Type 2/drug therapy; Enzyme Inhibitors/isolation & purification; Enzyme Inhibitors/pharmacology*; Humans; Hypoglycemic Agents/isolation & purification; Hypoglycemic Agents/pharmacology*; Plant Extracts/isolation & purification; Plant Extracts/pharmacology*; Orthosiphon/chemistry*; Glycoside Hydrolase Inhibitors*
The effect of dialect on the phonological analysis of Chinese-influenced Malaysian English speaking children

Phoon HS, Abdullah AC, Maclagan M

Int J Speech Lang Pathol, 2012 Dec;14(6):487-98.
PMID: 23039125 DOI: 10.3109/17549507.2012.719549

This study investigates the effect of dialect on phonological analyses in Chinese-influenced Malaysian English (ChME) speaking children. A total of 264 typically-developing ChME speaking children aged 3-7 years participated in this cross-sectional study. A single word naming task consisting of 195 words was used to elicit speech from the children. The samples obtained were transcribed phonetically and analysed descriptively and statistically. Phonological analyses were completed for speech sound accuracy, age of consonant acquisition, percentage of phonological process occurrence, and age of suppression for phonological processes. All these measurements differed based on whether or not ChME dialectal features were considered correct, with children gaining higher scores when ChME dialect features were considered correct. The findings of the present study provide guidelines for Malaysian speech-language pathologists and stress the need to appropriately consider ChME dialectal features in the phonological analysis of ChME speaking children. They also highlight the issues in accurate differential diagnosis of speech impairment for speech-language pathologists working with children from any linguistically diverse background.

MeSH terms: Age Factors; Child; Child Language*; Child, Preschool; Cross-Sectional Studies; Female; Humans; Language Tests; Malaysia; Male; Phonetics*; Reference Values; Speech Acoustics*; Speech Disorders/diagnosis; Speech Disorders/physiopathology; Speech Disorders/psychology; Speech-Language Pathology/methods; Speech Production Measurement; Voice Quality*; Reproducibility of Results; Multilingualism*
Assessing bilingual Chinese-English young children in Malaysia using language sample measures

Ooi CC, Wong AM

Int J Speech Lang Pathol, 2012 Dec;14(6):499-508.
PMID: 23039126 DOI: 10.3109/17549507.2012.712159

One reason why specific language impairment (SLI) is grossly under-identified in Malaysia is the absence of locally- developed norm-referenced language assessment tools for its multilingual and multicultural population. Spontaneous language samples provide quantitative information for language assessment, and useful descriptive information on child language development in complex language and cultural environments. This research consisted of two studies and investigated the use of measures obtained from English conversational samples among bilingual Chinese-English Malaysian preschoolers. The research found that the language sample measures were sensitive to developmental changes in this population and could identify SLI. The first study examined the relationship between age and mean length of utterance (MLU(w)), lexical diversity (D), and the index of productive syntax (IPSyn) among 52 typically-developing (TD) children aged between 3;4-6;9. Analyses showed a significant linear relationship between age and D (r = .450), the IPsyn (r = .441), and MLU(w) (r = .318). The second study compared the same measures obtained from 10 children with SLI, aged between 3;8-5;11, and their age-matched controls. The children with SLI had significantly shorter MLU(w) and lower IPSyn scores than the TD children. These findings suggest that utterance length and syntax production can be potential clinical markers of SLI in Chinese-English Malaysian children.

MeSH terms: Age Factors; Child; Child Language*; Child, Preschool; Female; Humans; Language Disorders/diagnosis*; Language Disorders/physiopathology; Language Disorders/psychology; Language Tests*; Malaysia; Male; Phonetics*; Predictive Value of Tests; Reference Values; Speech Acoustics*; Speech-Language Pathology/methods*; Speech Production Measurement*; Voice Quality*; Linear Models; Case-Control Studies; Multilingualism*
Fulltext An efficient clustering algorithm for partitioning Y-short tandem repeats data

Seman A, Bakar ZA, Isa MN

BMC Res Notes, 2012;5:557.
PMID: 23039132 DOI: 10.1186/1756-0500-5-557

Y-Short Tandem Repeats (Y-STR) data consist of many similar and almost similar objects. This characteristic of Y-STR data causes two problems with partitioning: non-unique centroids and local minima problems. As a result, the existing partitioning algorithms produce poor clustering results.

MeSH terms: Algorithms*; Alleles; Haplotypes*; Humans; Pattern Recognition, Automated/statistics & numerical data*; Cluster Analysis; Microsatellite Repeats*; Computational Biology/methods*; Databases, Genetic; Chromosomes, Human, Y*
Fulltext Enhanced cell disruption strategy in the release of recombinant hepatitis B surface antigen from Pichia pastoris using response surface methodology

Tam YJ, Allaudin ZN, Lila MA, Bahaman AR, Tan JS, Rezaei MA

BMC Biotechnol, 2012 Oct 05;12:70.
PMID: 23039947 DOI: 10.1186/1472-6750-12-70

BACKGROUND: Cell disruption strategies by high pressure homogenizer for the release of recombinant Hepatitis B surface antigen (HBsAg) from Pichia pastoris expression cells were optimized using response surface methodology (RSM) based on the central composite design (CCD). The factors studied include number of passes, biomass concentration and pulse pressure. Polynomial models were used to correlate the above mentioned factors to project the cell disruption capability and specific protein release of HBsAg from P. pastoris cells.
RESULTS: The proposed cell disruption strategy consisted of a number of passes set at 20 times, biomass concentration of 7.70 g/L of dry cell weight (DCW) and pulse pressure at 1,029 bar. The optimized cell disruption strategy was shown to increase cell disruption efficiency by 2-fold and 4-fold for specific protein release of HBsAg when compared to glass bead method yielding 75.68% cell disruption rate (CDR) and HBsAg concentration of 29.20 mg/L respectively.
CONCLUSIONS: The model equation generated from RSM on cell disruption of P. pastoris was found adequate to determine the significant factors and its interactions among the process variables and the optimum conditions in releasing HBsAg when validated against a glass bead cell disruption method. The findings from the study can open up a promising strategy for better recovery of HBsAg recombinant protein during downstream processing.

MeSH terms: Biomechanical Phenomena; Biophysics/methods*; Hepatitis B Surface Antigens/genetics*; Hepatitis B Surface Antigens/isolation & purification; Hepatitis B Surface Antigens/metabolism; Industrial Microbiology/methods*; Pichia/genetics*; Pichia/metabolism; Pichia/chemistry*; Pressure; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Gene Expression*
Fulltext People living with HIV/AIDS (PLWHA) and HIV/AIDS associated oral lesions; a study in Malaysia

Khan SA, Moorthy J, Omar H, Hasan SS

BMC Public Health, 2012;12:850.
PMID: 23043358 DOI: 10.1186/1471-2458-12-850

The continuous increase in number of people living with HIV/AIDS (PLWHA) represents a serious health and economic burden. HIV positive individuals with oral lesions have significantly lower oral health-related quality of life than HIV positive individuals without oral lesions. The objective of this study was to assess the knowledge, attitude and practices (KAP) within a cohort of HIV/AIDS positive patients towards HIV/AIDS associated oral lesions.

MeSH terms: Adult; Cross-Sectional Studies; Female; Follow-Up Studies; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Mouth Diseases/virology*; Patient Education as Topic; Surveys and Questionnaires; Program Evaluation; HIV Infections/complications*; Qualitative Research

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