Browse publications by year: 2015

  1. Nik Ramli NN, Omar N, Husin A, Ismail Z, Siran R
    Neurosci Lett, 2015 Feb 19;588:137-41.
    PMID: 25562631 DOI: 10.1016/j.neulet.2014.12.062
    Glutamate receptors are the integral cellular components associated with excitotoxicity mechanism induced by the ischemic cascade events. Therefore the glutamate receptors have become the major molecular targets of neuroprotective agents in stroke researches. Recent studies have demonstrated that a Group I metabotropic glutamate receptor agonist, (S)-3,5-dihydroxyphenylglycine ((S)-3,5-DHPG) preconditioning elicits neuroprotection in the hippocampal slice cultures exposed to toxic level of N-methyl-d-aspartate (NMDA). We further investigated the preconditioning effects of (S)-3,5-DHPG on acute ischemic stroke rats. One 10 or 100μM of (S)-3,5-DHPG was administered intrathecally to Sprague-Dawley adult male rats, 2h prior to induction of acute ischemic stroke by middle cerebral artery occlusion (MCAO). After 24h, neurological deficits were evaluated by modified stroke severity scores and grid-walking test. All rats were sacrificed and infarct volumes were determined by 2,3,5-triphenyltetrazolium chloride staining. The serum level of neuron-specific enolase (NSE) of each rat was analyzed by enzyme-linked immunosorbent assay (ELISA). One and 10μM of (S)-3,5-DHPG preconditioning in the stroke rats showed significant improvements in motor impairment (P<0.01), reduction in the infarct volume (P<0.01) and reduction in the NSE serum level (P<0.01) compared to the control stroke rats. We conclude that 1 and 10μM (S)-3,5-DHPG preconditioning induced protective effects against acute ischemic insult in vivo.
    MeSH terms: Animals; Brain Ischemia/etiology; Brain Ischemia/physiopathology; Brain Ischemia/prevention & control*; Glycine/analogs & derivatives*; Glycine/therapeutic use; Glycine/chemistry; Injections, Spinal; Male; Phosphopyruvate Hydratase/blood; Resorcinols/therapeutic use*; Resorcinols/chemistry; Stereoisomerism; Rats, Sprague-Dawley; Neuroprotective Agents/therapeutic use*; Neuroprotective Agents/chemistry; Infarction, Middle Cerebral Artery/complications; Infarction, Middle Cerebral Artery/physiopathology; Infarction, Middle Cerebral Artery/prevention & control*; Brain Infarction/etiology; Brain Infarction/pathology; Brain Infarction/prevention & control
  2. Byrne EM, Psychiatric Genetics Consortium Major Depressive Disorder Working Group, Raheja UK, Stephens SH, Heath AC, Madden PA, et al.
    J Clin Psychiatry, 2015 Feb;76(2):128-34.
    PMID: 25562672 DOI: 10.4088/JCP.14m08981
    OBJECTIVE: To test common genetic variants for association with seasonality (seasonal changes in mood and behavior) and to investigate whether there are shared genetic risk factors between psychiatric disorders and seasonality.

    METHOD: Genome-wide association studies (GWASs) were conducted in Australian (between 1988 and 1990 and between 2010 and 2013) and Amish (between May 2010 and December 2011) samples in whom the Seasonal Pattern Assessment Questionnaire (SPAQ) had been administered, and the results were meta-analyzed in a total sample of 4,156 individuals. Genetic risk scores based on results from prior large GWAS studies of bipolar disorder, major depressive disorder (MDD), and schizophrenia were calculated to test for overlap in risk between psychiatric disorders and seasonality.

    RESULTS: The most significant association was with rs11825064 (P = 1.7 × 10⁻⁶, β = 0.64, standard error = 0.13), an intergenic single nucleotide polymorphism (SNP) found on chromosome 11. The evidence for overlap in risk factors was strongest for schizophrenia and seasonality, with the schizophrenia genetic profile scores explaining 3% of the variance in log-transformed global seasonality scores. Bipolar disorder genetic profile scores were also associated with seasonality, although at much weaker levels (minimum P value = 3.4 × 10⁻³), and no evidence for overlap in risk was detected between MDD and seasonality.

    CONCLUSIONS: Common SNPs of large effect most likely do not exist for seasonality in the populations examined. As expected, there were overlapping genetic risk factors for bipolar disorder (but not MDD) with seasonality. Unexpectedly, the risk for schizophrenia and seasonality had the largest overlap, an unprecedented finding that requires replication in other populations and has potential clinical implications considering overlapping cognitive deficits in seasonal affective disorders and schizophrenia.

    MeSH terms: Adult; Aged; Alcoholism/genetics; Alcoholism/psychology; Bipolar Disorder/diagnosis; Bipolar Disorder/genetics*; Bipolar Disorder/psychology; Depressive Disorder, Major/diagnosis; Depressive Disorder, Major/genetics*; Depressive Disorder, Major/psychology; Diseases in Twins/diagnosis; Diseases in Twins/genetics; Diseases in Twins/psychology; Female; Genotype; Humans; Life Change Events; Male; Middle Aged; Psychiatric Status Rating Scales; Queensland; Schizophrenia/diagnosis; Schizophrenia/genetics*; Schizophrenic Psychology*; United States; Multifactorial Inheritance/genetics*; Young Adult; Amish/genetics; Amish/psychology
  3. Kuok SS, Ramli N, Tan CT, Goh KJ
    Clin Neurol Neurosurg, 2015 Mar;130:8-10.
    PMID: 25562719 DOI: 10.1016/j.clineuro.2014.12.003
    MeSH terms: Adult; Brain/pathology*; Diagnosis, Differential; Humans; Male; Masticatory Muscles/physiopathology*; Melkersson-Rosenthal Syndrome/complications*; Melkersson-Rosenthal Syndrome/diagnosis
  4. Jegannathan B, Kullgren G, Deva P
    Asian J Psychiatr, 2015 Feb;13:75-80.
    PMID: 25563073 DOI: 10.1016/j.ajp.2014.12.006
    Cambodia had suffered enormously due to war and internecine conflict during the latter half of the twentieth century, more so during the Vietnam War. Total collapse of education and health systems during the Pol Pot era continues to be a challenge for developing the necessary infrastructure and human resources to provide basic minimum mental health care which is compounded by the prevailing cultural belief and stigma over mental, neurological and substance abuse disorders (MNSDs). The mental health research and services in Cambodia had been predominantly 'trauma focused', a legacy of war, and there is a need to move toward epidemiologically sound public health oriented mental health policy and service development. Integrating mental health program with primary health care services with specifically stated minimum package of activities at primary level and complementary package of activities at secondary level is an opportunity to meet the needs and rights of persons with mental, neurological and substance abuse disorders (PWMNSDs) in Cambodia, provided there is mental health leadership, government commitment and political will.
    MeSH terms: Mental Disorders/therapy*; Cambodia; Health Policy; Health Services Accessibility*; Humans; Mental Health Services*
  5. Khoo EM, Sararaks S, Lee WK, Liew SM, Cheong AT, Abdul Samad A, et al.
    Asia Pac J Public Health, 2015 Sep;27(6):670-7.
    PMID: 25563351 DOI: 10.1177/1010539514564007
    This study aimed to develop an intervention to reduce medical errors and to determine if the intervention can reduce medical errors in public funded primary care clinics. A controlled interventional trial was conducted in 12 conveniently selected primary care clinics. Random samples of outpatient medical records were selected and reviewed by family physicians for documentation, diagnostic, and management errors at baseline and 3 months post intervention. The intervention package comprised educational training, structured process change, review methods, and patient education. A significant reduction was found in overall documentation error rates between intervention (Pre 98.3% [CI 97.1-99.6]; Post 76.1% [CI 68.1-84.1]) and control groups (Pre 97.4% [CI 95.1-99.8]; Post 89.5% [85.3-93.6]). Within the intervention group, overall management errors reduced from 54.0% (CI 49.9-58.0) to 36.6% (CI 30.2-43.1) and medication error from 43.2% (CI 39.2-47.1) to 25.2% (CI 19.9-30.5). This low-cost intervention was useful to reduce medical errors in resource-constrained settings.
    MeSH terms: Ambulatory Care Facilities/organization & administration*; Ambulatory Care Facilities/statistics & numerical data; Humans; Malaysia; Patient Education as Topic; Primary Health Care/organization & administration*; Primary Health Care/statistics & numerical data; Teaching; Program Evaluation; Public Sector/organization & administration*; Public Sector/statistics & numerical data; Medical Errors/prevention & control*
  6. Lee LH, Azman AS, Zainal N, Yin WF, Mutalib NA, Chan KG
    Int J Syst Evol Microbiol, 2015 Mar;65(Pt 3):996-1002.
    PMID: 25563924 DOI: 10.1099/ijs.0.000053
    Strain MUSC 117(T) was isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish-white pigmented, Gram-staining-positive, rod-coccus shaped and non-motile. On the basis of 16S rRNA gene sequence, strain MUSC 117(T) exhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127(T) (98.0 %), Sinomonas albida LC13(T) (97.9 %) and Sinomonas soli CW 59(T) (97.8 %), and lower (<97.6 %) sequence similarity to other species of the genus Sinomonas. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 27 %) between strain MUSC 117(T) and closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and a smaller amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipids. The major fatty acids (>10.0 %) of the cell membrane were anteiso-C15 : 0 (39.4 %), C18 : 1ω7c (17.7 %), anteiso-C17 : 0 (17.2 %) and iso-C16 : 0 (11.4 %). The predominant respiratory quinones detected were MK-9(H2) and MK-9. The DNA G+C content was 67.3 mol%. A comparison of BOX-PCR fingerprints indicated that strain MUSC 117(T) represented a unique DNA profile. Results based on a polyphasic approach showed that strain MUSC 117(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi sp. nov. is MUSC 117(T) ( = DSM 29362(T) = MCCC 1K00410(T) = NBRC 110653(T)).
    MeSH terms: Base Composition; Cell Wall/chemistry; DNA, Bacterial/genetics; Fatty Acids/chemistry; Glycolipids/chemistry; Malaysia; Micrococcaceae/classification*; Micrococcaceae/genetics; Micrococcaceae/isolation & purification; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan/chemistry; Phylogeny*; RNA, Ribosomal, 16S/genetics; Soil Microbiology*; Bacterial Typing Techniques; Sequence Analysis, DNA; Avicennia/microbiology*; Forests*
  7. Shaharuddin S, Muhamad II
    Carbohydr Polym, 2015 Mar 30;119:173-81.
    PMID: 25563958 DOI: 10.1016/j.carbpol.2014.11.045
    The aim of this research was to enhance the survivability of Lactobacillus rhamnosus NRRL 442 against heat exposure via a combination of immobilization and microencapsulation processes using sugarcane bagasse (SB) and sodium alginate (NaA), respectively. The microcapsules were synthesized using different alginate concentration of 1, 2 and 3% and NaA:SB ratio of 1:0, 1:1 and 1:1.5. This beneficial step of probiotic immobilization before microencapsulation significantly enhanced microencapsulation efficiency and cell survivability after heat exposure of 90°C for 30s. Interestingly, the microcapsule of SB-immobilized probiotic could obtain protection from heat using microencapsulation of NaA concentration as low as 1%. SEM images illustrated the incorporation of immobilized L. rhamnosus within alginate matrices and its changes after heat exposure. FTIR spectra confirmed the change in functional bonding in the presence of sugarcane bagasse, probiotic and alginate. The results demonstrated a great potential in the synthesis of heat resistant microcapsules for probiotic.
    MeSH terms: Adaptation, Physiological/drug effects*; Alginates/pharmacology*; Capsules; Cellulose/pharmacology*; Drug Compounding/methods*; Hexuronic Acids/pharmacology; Microscopy, Electron, Scanning; Particle Size; Surface Properties; Temperature*; Spectroscopy, Fourier Transform Infrared; Cells, Immobilized/cytology; Cells, Immobilized/drug effects; Glucuronic Acid/pharmacology; Microbial Viability/drug effects*; Lactobacillus rhamnosus/cytology*; Lactobacillus rhamnosus/drug effects
  8. Idriss AA, Ahmad AK
    Bull Environ Contam Toxicol, 2015 Feb;94(2):204-8.
    PMID: 25564001 DOI: 10.1007/s00128-014-1452-x
    This study examined the concentration of heavy metals in 13 fish species. The results indicated that shellfish species (clams) have the highest metal concentrations, followed by demersal and pelagic fishes. The mean concentration of metals in clams are Zn 88.74 ± 11.98 µg/g, Cu 4.96 ± 1.06 µg/g, Pb 1.22 ± 0.19 µg/g, Cd 0.34 ± 0.04 µg/g dry wt. basis, whereas the same measure in fish tissues was 58.04 ± 18.51, 2.47 ± 1.21, 0.58 ± 0.27 and 0.17 ± 0.08 µg/g dry wt. basis. The concentrations of heavy metals in clams and fish tissues were still lower than the maximum allowable concentrations as suggested by the Malaysian Food Act (1983) and are considered safe for local human consumption.
    MeSH terms: Animals; Environmental Monitoring*; Fishes/metabolism*; Humans; Water Pollutants, Chemical/chemistry*; Metals, Heavy/metabolism*; Metals, Heavy/chemistry*; Rivers/chemistry*
  9. Lee CC, Harun F, Jalaludin MY, Heh CH, Othman R, Junit SM
    BMJ Open, 2015 Jan 05;5(1):e006121.
    PMID: 25564141 DOI: 10.1136/bmjopen-2014-006121
    OBJECTIVES: The c.2268dup mutation in the thyroid peroxidase (TPO) gene is the most common TPO alteration reported in Taiwanese patients with thyroid dyshormonogenesis. The ancestors of these patients are believed to originate from the southern province of China. Our previous study showed that this mutation leads to reduced abundance of the TPO protein and loss of TPO enzyme activity in a Malaysian-Chinese family with goitrous hypothyroidism. The aim of our study was to provide further data on the incidence of the c.2268dup mutation in a cohort of Malaysian-Chinese and its possible phenotypic effects.

    SETTING: Cohort study.

    PARTICIPANTS: Twelve biologically unrelated Malaysian-Chinese patients with congenital hypothyroidism were recruited in this study. All patients showed high thyrotropin and low free thyroxine levels at the time of diagnosis with proven presence of a thyroid gland.

    PRIMARY OUTCOME MEASURE: Screening of the c.2268dup mutation in the TPO gene in all patients was carried out using a PCR-direct DNA sequencing method.

    SECONDARY OUTCOME MEASURE: Further screening for mutations in other exonic regions of the TPO gene was carried out if the patient was a carrier of the c.2268dup mutation.

    RESULTS: The c.2268dup mutation was detected in 4 of the 12 patients. Apart from the c.2268dup and a previously documented mutation (c.2647C>T), two novel TPO alterations, c.670_672del and c.1186C>T, were also detected in our patients. In silico analyses predicted that the novel alterations affect the structure/function of the TPO protein.

    CONCLUSIONS: The c.2268dup mutation was detected in approximately one-third of the Malaysian-Chinese patients with thyroid dyshormonogenesis. The detection of the novel c.670_672del and c.1186C>T alterations expand the mutation spectrum of TPO associated with thyroid dyshormonogenesis.

    MeSH terms: China/ethnology; Congenital Hypothyroidism/enzymology*; Congenital Hypothyroidism/genetics*; Congenital Hypothyroidism/epidemiology; Exons; Female; Goiter/enzymology; Goiter/genetics*; Humans; Infant; Infant, Newborn; Iodide Peroxidase/genetics*; Malaysia/epidemiology; Male; Mutation*; Cohort Studies; Prevalence; Sequence Analysis, DNA/methods; Genetic Predisposition to Disease/genetics*; Asian Continental Ancestry Group/genetics
  10. Martin AG, Abdullah JY, Jaafar A, Ghani AR, Rajion ZA, Abdullah JM
    J Clin Neurosci, 2015 Apr;22(4):735-9.
    PMID: 25564264 DOI: 10.1016/j.jocn.2014.09.021
    Decompressive craniectomy (DC) is a surgical option in managing uncontrolled raised intracranial pressure refractory to medical therapy. The authors evaluate the addition of zygomatic arch (ZA) resection with standard DC and analyze the resulting increase in brain volume using three-dimensional volumetric CT scans. Measurements of brain expansion dimension morphometrics from CT images were also analyzed. Eighteen patients were selected and underwent DC with ZA resection. The pre- and post-operative CT images were analyzed for volume and dimensional changes. CT images of 29 patients previously operated on at the same center were retrieved from the picture archiving and communication system (PACS) and were similarly studied. The findings obtained from the two groups were compared and analyzed. Analysis from three-dimensional CT volumetric techniques revealed an significant increase of 27.97ml (95% confidence interval [CI]: 39.98-180.36; p=0.048) when compared with standard DC. Brain expansion analysis of maximum hemicraniectomy diameter revealed a mean difference of 0.82cm (95% CI: 0.25-1.38; p=0.006). Analysis of the ratio of maximum hemicraniectomy diameter to maximum anteroposterior diameter gave a mean difference of 0.04 (95% CI: 0.05-0.07; p=0.026). The addition of ZA resection to standard DC may prove valuable in terms of absolute brain volume gain. This technique is comparable to other maneuvers used to provide maximum brain expansion in the immediate post-operative period.
    MeSH terms: Adolescent; Adult; Aged; Brain/radiography; Brain/surgery*; Female; Humans; Male; Middle Aged; Organ Size; Postoperative Period; Tomography, X-Ray Computed; Zygoma/radiography; Zygoma/surgery*; Treatment Outcome; Intracranial Hypertension/radiography; Intracranial Hypertension/surgery*; Young Adult; Decompressive Craniectomy/methods*
  11. Harston GW, Tee YK, Blockley N, Okell TW, Thandeswaran S, Shaya G, et al.
    Brain, 2015 Jan;138(Pt 1):36-42.
    PMID: 25564491 DOI: 10.1093/brain/awu374
    The original concept of the ischaemic penumbra suggested imaging of regional cerebral blood flow and metabolism would be required to identify tissue that may benefit from intervention. Amide proton transfer magnetic resonance imaging, a chemical exchange saturation transfer technique, has been used to derive cerebral intracellular pH in preclinical stroke models and has been proposed as a metabolic marker of ischaemic penumbra. In this proof of principle clinical study, we explored the potential of this pH-weighted magnetic resonance imaging technique at tissue-level. Detailed voxel-wise analysis was performed on data from a prospective cohort of 12 patients with acute ischaemic stroke. Voxels within ischaemic core had a more severe intracellular acidosis than hypoperfused tissue recruited to the final infarct (P < 0.0001), which in turn was more acidotic than hypoperfused tissue that survived (P < 0.0001). In addition, when confined to the grey matter perfusion deficit, intracellular pH (P < 0.0001), but not cerebral blood flow (P = 0.31), differed between tissue that infarcted and tissue that survived. Within the presenting apparent diffusion coefficient lesion, intracellular pH differed between tissue with early apparent diffusion lesion pseudonormalization and tissue with true radiographic recovery. These findings support the need for further investigation of pH-weighted imaging in patients with acute ischaemic stroke.
    MeSH terms: Aged, 80 and over; Analysis of Variance; Brain Ischemia/complications*; Cerebrovascular Circulation/physiology*; Female; Humans; Image Processing, Computer-Assisted; Male; Middle Aged; Cohort Studies; Brain Infarction/etiology; Stroke/diagnosis*; Stroke/etiology*; Diffusion Magnetic Resonance Imaging
  12. Chua KH, Lian LH, Khor WC, Lee WS, Hilmi I, Goh KL, et al.
    J Dig Dis, 2015 Apr;16(4):205-16.
    PMID: 25564941 DOI: 10.1111/1751-2980.12229
    The study aimed to investigate the association between the interferon regulatory factor 5 (IRF5) gene polymorphisms and the onset of Crohn's disease (CD) in a Malaysian cohort.
    MeSH terms: Adult; Alleles; Crohn Disease/genetics*; Female; Genotype; Humans; Malaysia; Male; Middle Aged; Risk Factors; Cohort Studies; Case-Control Studies; Age of Onset; Genetic Predisposition to Disease/genetics*; Polymorphism, Single Nucleotide/genetics*; Asian Continental Ancestry Group/genetics*; Interferon Regulatory Factors/genetics*; Real-Time Polymerase Chain Reaction
  13. Tan HY, Yong YK, Andrade BB, Shankar EM, Ponnampalavanar S, Omar SF, et al.
    AIDS, 2015 Feb 20;29(4):421-31.
    PMID: 25565499 DOI: 10.1097/QAD.0000000000000557
    Tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is a substantial problem in HIV/TB coinfected patients commencing antiretroviral therapy (ART). The immunopathogenesis of TB-IRIS includes increased production of proinflammatory chemokines and cytokines, including interleukin-18, which is a signature cytokine of the nucleotide-binding domain and leucine-rich repeat pyrin containing protein-3 inflammasome. We compared plasma levels of interleukin-18 and other biomarkers of monocyte/macrophage activation in the prediction and characterization of TB-IRIS.
    MeSH terms: Adult; Antigens, Bacterial/immunology; Antitubercular Agents/therapeutic use; Female; Humans; Immunity, Innate/immunology*; Male; Prospective Studies; Tuberculosis/blood; Tuberculosis/immunology*; Tuberculosis/physiopathology; Biomarkers/blood; HIV Infections/blood; HIV Infections/immunology*; HIV Infections/physiopathology; CD4 Lymphocyte Count; Anti-HIV Agents/therapeutic use; Interleukin-18/blood; Interleukin-18/immunology*; Immune Reconstitution Inflammatory Syndrome/immunology*; Immune Reconstitution Inflammatory Syndrome/physiopathology; Coinfection
  14. Momtaz YA, Haron SA, Hamid TA, Ibrahim R, Masud J
    Clin Interv Aging, 2015;10:49-53.
    PMID: 25565786 DOI: 10.2147/CIA.S69220
    Despite several studies attempting to identify the risk factors for dementia, little is known about the impact of childhood living conditions on cognitive function in later life. The present study aims to examine the unique contribution of food insufficiency in childhood to dementia in old age.
    MeSH terms: Age Factors; Aged; Child; Cross-Sectional Studies; Female; Humans; Intelligence Tests; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Social Conditions/statistics & numerical data; Socioeconomic Factors; Time; Geriatric Assessment; Prevalence
  15. Ebrahimiasl S, Zakaria A, Kassim A, Basri SN
    Int J Nanomedicine, 2015;10:217-27.
    PMID: 25565815 DOI: 10.2147/IJN.S69740
    An antibacterial and conductive bionanocomposite (BNC) film consisting of polypyrrole (Ppy), zinc oxide (ZnO) nanoparticles (NPs), and chitosan (CS) was electrochemically synthesized on indium tin oxide (ITO) glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO-Ppy/CS and ITO-Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%), while it was increased gradually for higher ratios (10, 15, and 20 wt%). The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa. The thermal stability and physical properties of BNC films were increased by an increase in the weight ratio of ZnO NPs, promising novel applications for the electrically conductive polysaccharide-based nanocomposites, particularly those that may exploit the antimicrobial nature of Ppy/ZnO/CS BNCs.
  16. Mohamad Zamberi Z, Zakaria Z, Abdul Aziz AT, Heng BS, Zaid M, Chong CL, et al.
    PMID: 25566870 DOI: 10.1186/s12952-014-0020-6
    Dengue is a major public health problem in many tropical and sub-tropical countries. Vascular leakage and shock are identified as the major causes of deaths in patients with severe dengue. Studies have suggested the potential role of Fc gamma receptors I (FcγRI) in the pathogenesis of dengue. We hypothesized that the circulating level of Fcγ receptor I could potentially be used as an indicator in assisting early diagnosis of severe dengue.
    MeSH terms: Adolescent; Adult; Child; Dengue/blood*; Dengue/diagnosis*; Female; Humans; Immunoglobulin G/blood*; Male; Middle Aged; Cohort Studies; Biomarkers/blood; Receptors, IgG/blood*; Young Adult; Vascular System Injuries/blood*; Vascular System Injuries/diagnosis*
  17. Cao N, Zhao A, Zhao G, Wang X, Han B, Lin R, et al.
    Integr Cancer Ther, 2015 Mar;14(2):133-9.
    PMID: 25567328 DOI: 10.1177/1534735414564185
    BACKGROUND: In China, traditional Chinese herbal medicine (TCHM) has been widely used for pancreatic cancer. This retrospective, matched case-control study aimed to assess factors affecting the survival time of patients with pancreatic cancer.
    METHODS: From 2004 to 2012, a total of 411 patients with pathologically confirmed pancreatic cancer were enrolled, and 272 patients were matched and divided into TCHM and non-TCHM groups (control group) based on received TCHM or not. The match was according to gender, age of onset, radiotherapy, and chemotherapy. Both groups received comprehensive treatments, the TCHM group simultaneously received the TCHM spleen-invigorating compound for more than 3 months. The Cox model was used for prognostic factor analysis and the Kaplan-Meier method for estimating median overall survival (OS) and disease-free survival (DFS).
    RESULTS: In 130 patients with advanced pancreatic cancer, COX analysis showed the Karnofsky Performance Scale (KPS; P = .000), radiotherapy (P = .003), and TCHM (P = .001) were independent prognostic factors for OS, with median OS of 12.7 and 9.9 months in TCHM and non-TCHM groups, respectively (hazard ratio [HR] = 0.520; 95% confidence interval [CI] = 0.353-0.766; P = .033). In 142 patients undergoing radical surgery, KPS (P = .000) and TCHM (P = .000) were independent prognostic factors for OS and DFS, median OS was 23.8 and 12.4 months in TCHM and non-TCHM groups, respectively (HR = 0.373; 95% CI = 0.251-0.554; P = .000), and the median DFS was 21.5 and 10.2 months in TCHM and non-TCHM groups, respectively (HR = 0.352; 95% CI = 0.237-0.522; P = .000).
    CONCLUSIONS: KPS was an important prognostic factor of pancreatic cancer. Spleen-invigorating compounds could have an effect on improving the prognosis of pancreatic cancer patients.
    MeSH terms: Aged; China; Combined Modality Therapy; Female; Follow-Up Studies; Humans; Male; Medicine, Chinese Traditional/methods*; Middle Aged; Pancreatic Neoplasms/pathology; Pancreatic Neoplasms/therapy*; Prognosis; Retrospective Studies; Survival Rate; Proportional Hazards Models; Survival Analysis; Case-Control Studies; Treatment Outcome; Karnofsky Performance Status; Disease-Free Survival; Kaplan-Meier Estimate
  18. HIV-CAUSAL Collaboration, Cain LE, Phillips A, Olson A, Sabin C, Jose S, et al.
    Clin Infect Dis, 2015 Apr 15;60(8):1262-8.
    PMID: 25567330 DOI: 10.1093/cid/ciu1167
    BACKGROUND: Current clinical guidelines consider regimens consisting of either ritonavir-boosted atazanavir or ritonavir-boosted lopinavir and a nucleoside reverse transcriptase inhibitor (NRTI) backbone among their recommended and alternative first-line antiretroviral regimens. However, these guidelines are based on limited evidence from randomized clinical trials and clinical experience.

    METHODS: We compared these regimens with respect to clinical, immunologic, and virologic outcomes using data from prospective studies of human immunodeficiency virus (HIV)-infected individuals in Europe and the United States in the HIV-CAUSAL Collaboration, 2004-2013. Antiretroviral therapy-naive and AIDS-free individuals were followed from the time they started a lopinavir or an atazanavir regimen. We estimated the 'intention-to-treat' effect for atazanavir vs lopinavir regimens on each of the outcomes.

    RESULTS: A total of 6668 individuals started a lopinavir regimen (213 deaths, 457 AIDS-defining illnesses or deaths), and 4301 individuals started an atazanavir regimen (83 deaths, 157 AIDS-defining illnesses or deaths). The adjusted intention-to-treat hazard ratios for atazanavir vs lopinavir regimens were 0.70 (95% confidence interval [CI], .53-.91) for death, 0.67 (95% CI, .55-.82) for AIDS-defining illness or death, and 0.91 (95% CI, .84-.99) for virologic failure at 12 months. The mean 12-month increase in CD4 count was 8.15 (95% CI, -.13 to 16.43) cells/µL higher in the atazanavir group. Estimates differed by NRTI backbone.

    CONCLUSIONS: Our estimates are consistent with a lower mortality, a lower incidence of AIDS-defining illness, a greater 12-month increase in CD4 cell count, and a smaller risk of virologic failure at 12 months for atazanavir compared with lopinavir regimens.

    MeSH terms: Atazanavir Sulfate/therapeutic use*; Adolescent; Adult; Cooperative Behavior; Europe; Female; Humans; Male; Middle Aged; Prospective Studies; United States; Cohort Studies; HIV Infections/drug therapy*; HIV Infections/immunology; HIV Infections/virology; Treatment Outcome; CD4 Lymphocyte Count; Developed Countries; Anti-HIV Agents/therapeutic use*; Viral Load; Antiretroviral Therapy, Highly Active/methods*; Young Adult; Lopinavir/therapeutic use*
  19. Teoh BT, Sam SS, Tan KK, Danlami MB, Shu MH, Johari J, et al.
    J Clin Microbiol, 2015 Mar;53(3):830-7.
    PMID: 25568438 DOI: 10.1128/JCM.02648-14
    A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without cross-reacting with other arboviruses. We assessed the diagnostic performance of the RT-RPA assay for the detection of DENV RNA in 203 serum samples of patients with clinically suspected dengue. The sera were simultaneously tested for DENV using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and IgG-capture enzyme-linked immunosorbent assays (ELISA). Acute DENV infection was confirmed in 130 samples and 61 of the samples (46.9%) were classified as viremic with qRT-PCR. The RT-RPA assay showed good concordance (κ of ≥0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic samples. When used in combination with ELISA, both the RT-RPA and RT-LAMP assays increased the detection of acute DENV infection to ≥95.7% (≥45/47) in samples obtained within 5 days of illness. The results from the study suggest that the RT-RPA assay is the most rapid molecular diagnostic tool available for the detection of DENV. Hence, it is possible to use the RT-RPA assay in a laboratory to complement routine serology testing for dengue.
    MeSH terms: Adolescent; Adult; Aged; Child; Child, Preschool; Dengue/diagnosis*; Dengue/virology; Dengue Virus/genetics; Dengue Virus/isolation & purification*; Female; Genotype; Humans; Male; Middle Aged; RNA, Viral/analysis; RNA, Viral/genetics; Sensitivity and Specificity; Time Factors; Cohort Studies; Reverse Transcriptase Polymerase Chain Reaction/methods*; Molecular Diagnostic Techniques/methods*; Early Diagnosis; Young Adult
  20. Omar H, Lim CR, Chao S, Lee MM, Bong CW, Ooi EJ, et al.
    J Clin Gastroenterol, 2015 Feb;49(2):150-7.
    PMID: 25569223 DOI: 10.1097/MCG.0000000000000112
    Up to 25% of chronic hepatitis B (CHB) patients eventually develop hepatocellular carcinoma (HCC), a disease with poor prognosis unless detected early. This study identifies a blood-based RNA biomarker panel for early HCC detection in CHB.
    MeSH terms: Adult; Female; Genetic Testing*; Carcinoma, Hepatocellular/blood; Carcinoma, Hepatocellular/diagnosis*; Carcinoma, Hepatocellular/genetics*; Carcinoma, Hepatocellular/virology; Humans; Liver Neoplasms/blood; Liver Neoplasms/diagnosis*; Liver Neoplasms/genetics*; Liver Neoplasms/virology; Malaysia; Male; Middle Aged; Predictive Value of Tests; RNA, Neoplasm/blood; RNA, Neoplasm/genetics*; ROC Curve; Biomarkers, Tumor/blood; Biomarkers, Tumor/genetics*; Reproducibility of Results; Gene Expression Regulation, Neoplastic; Case-Control Studies; Area Under Curve; Hepatitis B, Chronic/complications*; Hepatitis B, Chronic/diagnosis; Reverse Transcriptase Polymerase Chain Reaction; Oligonucleotide Array Sequence Analysis; Gene Expression Profiling; Databases, Genetic; Gene Regulatory Networks; Early Detection of Cancer/methods*; Genome-Wide Association Study; Real-Time Polymerase Chain Reaction
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