Browse publications by year: 2016

  1. Jeyachelvi K, Juwita S, Norwati D
    Asian Pac J Cancer Prev, 2016;17(8):3983-8.
    PMID: 27644649
    BACKGROUND: Cervical cancer though preventable is still the leading cause of cancer death among women secondary to breast cancer. Persistent infection with HPV has been causally linked to the disease. A school based HPV vaccination program was introduced in late 2010 in Malaysia and nurse support is essential for its success.
    OBJECTIVES: To determine nurses knowledge and attitudes about HPV infection and its vaccines, and factors associated with their knowledge.
    MATERIALS AND METHODS: This cross-sectional study was conducted among nurses working at primary health clinics in Kelantan from mid-June till the end of July 2014. Its involved 330 nurses selected through multistage random sampling. A validated self-administered questionnaire consisting of 11 items for the knowledge domain and eight items for the attitude domain was used.
    RESULTS: The response rate of the study was 93.7%. The mean knowledge and mean attitude (SD) scores were 5.37 (1.76) and 29.8 (3.51) respectively. Only 24% knew that HPV is the most common sexually transmitted infection and 67% correctly answered that Gardasil vaccine can protect against four types of HPV. Nearly 60% of participants wrongly answered that HPV vaccines cannot be offered to sexually active women. Likewise, 70.9% participants were not aware that HPV vaccine may be appropriate for females aged 9 through 26 years. Though 90% of participants believed that the vaccine is safe, nearly half of them were unsure about efficacy. From multiple linear regression analysis, among the factors tested only participant's level of education showed a statistically significant association with the HPV knowledge score (<0.001).
    CONCLUSIONS: This study indicates nurses have favorable attitudes towards HPV vaccination; however they have significant knowledge deficit and major misunderstanding in critical knowledge items. Among the factors tested, nursing qualification is the only factor that is significantly associated with the nurses knowledge score.
    MeSH terms: Adult; Ambulatory Care Facilities; Awareness; Uterine Cervical Neoplasms/immunology; Uterine Cervical Neoplasms/prevention & control; Uterine Cervical Neoplasms/psychology; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice; Malaysia; Nurses/psychology*; Patient Acceptance of Health Care/psychology*; Primary Health Care/methods; Surveys and Questionnaires; Vaccination/psychology*; Immunization Programs/methods; Papillomavirus Infections/immunology*; Papillomavirus Infections/psychology*; Papillomavirus Vaccines/immunology*
  2. Rahman FA, Naidu J, Ngiu CS, Yaakob Y, Mohamed Z, Othman H, et al.
    Asian Pac J Cancer Prev, 2016;17(8):4037-41.
    PMID: 27644658
    BACKGROUND: Hepatocellular carcinoma (HCC) is a common cancer that is frequently diagnosed at an advanced stage. Transarterial chemoembolisation (TACE) is an effective palliative treatment for patients who are not eligible for curative treatment. The two main methods for performing TACE are conventional (c-TACE) or with drug eluting beads (DEB-TACE). We sought to compare survival rates and tumour response between patients undergoing c-TACE and DEB-TACE at our centre.

    MATERIALS AND METHODS: A retrospective cohort study of patients undergoing either treatment was carried out from January 2009 to December 2014. Tumour response to the procedures was evaluated according to the modified Response Evaluation Criteria in Solid Tumors (mRECIST). Kaplan-Meier analysis was used to assess and compare the overall survival in the two groups.

    RESULTS: A total of 79 patients were analysed (34 had c-TACE, 45 had DEB-TACE) with a median follow-up of 11.8 months. A total of 20 patients in the c-TACE group (80%) and 12 patients in the DEB-TACE group (44%) died during the follow up period. The median survival durations in the c-TACE and DEB-TACE groups were 4.9 ± 3.2 months and 8.3 ± 2.0 months respectively (p=0.008). There was no statistically significant difference noted among the two groups with respect to mRECIST criteria.

    CONCLUSIONS: DEB-TACE demonstrated a significant improvement in overall survival rates for patients with unresectable HCC when compared to c-TACE. It is a safe and promising approach and should potentially be considered as a standard of care in the management of unresectable HCC.

    MeSH terms: Adult; Aged; Aged, 80 and over; Doxorubicin/administration & dosage*; Female; Carcinoma, Hepatocellular/pathology; Carcinoma, Hepatocellular/therapy*; Humans; Liver Neoplasms/pathology; Liver Neoplasms/therapy*; Malaysia; Male; Middle Aged; Retrospective Studies; Survival Rate; Chemoembolization, Therapeutic/methods; Treatment Outcome; Kaplan-Meier Estimate; Young Adult; Tertiary Care Centers; Response Evaluation Criteria in Solid Tumors
  3. Yen SH, Knight A, Krishna M, Muda W, Rufai A
    Asian Pac J Cancer Prev, 2016;17(8):4083-8.
    PMID: 27644665
    BACKGROUND: Physical inactivity has been identified as the fourth leading risk factor for global mortality and is associated with increased breast cancer diagnosis and recurrence.

    PURPOSE: To examine the association between adult lifetime physical activity and breast cancer risk in a case-control analysis.

    MATERIALS AND METHODS: This study involved 122 cases of breast cancer and 121 controls in the state of Kelantan in Malaysia. A comprehensive measure of lifetime physical activity was used to assess occupational, household, and recreational/sports activity. For every type of activity, a metabolic equivalent (MET) score was assigned using the compendium of physical activities. MET-hours/week per year for all types of activities at different levels of intensities for different age groups were calculated. Logistic regression analysis was used to estimate odds ratios between various measures of physical activity and breast cancer risk.

    CONCLUSIONS: The mean MET-hours/week per year for all activities were 120.0 and 132.9 of MET-hours/week per year for cases and controls respectively. Household activities accounted for about 70% of the total lifetime physical activities. Only about 2.5% of the total lifetime physical activities were in the form of recreational/sports. This study found no association between lifetime occupational and recreational/sports physical activities with breast cancer risk among Kelantanese women. However, higher intensity lifetime household activities seemed to significantly reduce risk of breast cancer.

    MeSH terms: Breast Neoplasms/epidemiology*; Breast Neoplasms/physiopathology*; Female; Humans; Malaysia/epidemiology; Middle Aged; Neoplasm Recurrence, Local/epidemiology; Neoplasm Recurrence, Local/physiopathology; Risk Factors; Exercise/physiology*; Case-Control Studies
  4. Ramli R, Oxley J
    Injury, 2016 Nov;47(11):2442-2449.
    PMID: 27645615 DOI: 10.1016/j.injury.2016.09.022
    INTRODUCTION: In Malaysia, motorcyclists continue to outnumber other road users in injuries and deaths. The objective of this study was to determine the association between helmet fixation and helmet type with head injury and severity of head injury among Malaysian motorcyclists.

    METHODS: The study design was a prospective cross-sectional study. The participants involved injured motorcyclists who were admitted in five selected hospitals in Klang Valley, Malaysia. Participants who sustained head injury were selected as the cases while those with injury below the neck (IBN) were selected as the controls. Questionnaire comprising motorcyclist, vehicle, helmet and crash factors was examined. Diagnoses of injuries were obtained from the participants' medical records.

    RESULTS: The total subjects with head injuries were 404 while those with IBN were 235. Majority of the cases (76.2%) and controls (80.4%) wore the half-head and open-face helmets, followed by the tropical helmets (5.4% and 6.0% of the cases and controls, respectively). Full-face helmets were used by 1.2% of the cases and 4.7% of the controls. 5.7% of the cases and 6.0% of the controls did not wear a helmet. 32.7% of the cases and 77.4% of the controls had their helmets fixed. Motorcyclists with ejected helmets were five times as likely to sustain head injury [adjusted odds ratio, AOR 5.73 (95% CI 3.38-9.73)] and four times as likely to sustain severe head injury [AOR of 4.83 (95% CI 2.76-8.45)]. The half head and open face helmets had AOR of 0.24 (95% CI 0.10-0.56) for severe head injury when compared to motorcyclists who did not wear a helmet.

    CONCLUSION: Helmet fixation is more effective than helmet type in providing protection to the motorcyclists.

    MeSH terms: Accidents, Traffic/mortality; Accidents, Traffic/statistics & numerical data*; Adolescent; Adult; Cross-Sectional Studies; Facial Injuries/mortality; Facial Injuries/prevention & control*; Female; Craniocerebral Trauma/mortality; Craniocerebral Trauma/prevention & control*; Head Protective Devices/adverse effects; Head Protective Devices/utilization*; Humans; Health Knowledge, Attitudes, Practice; Malaysia/epidemiology; Male; Middle Aged; Motorcycles*; Prospective Studies; Surveys and Questionnaires; Risk Factors; Odds Ratio; Young Adult
  5. Trache D, Hussin MH, Hui Chuin CT, Sabar S, Fazita MR, Taiwo OF, et al.
    Int J Biol Macromol, 2016 Dec;93(Pt A):789-804.
    PMID: 27645920 DOI: 10.1016/j.ijbiomac.2016.09.056
    Considering its widespread usage in various fields, such as food, pharmaceutical, medical, cosmetic and polymer composites industries, microcrystalline cellulose (MCC) is becoming impellent due to increasing demand of alternatives to non-renewable and scarce fossil materials. Although it still suffers from some drawbacks, MCC has recently gained more interest owing to its renewability, non-toxicity, economic value, biodegradability, high mechanical properties, high surface area and biocompatibility. New sources, new isolation processes, and new treatments are currently under development to satisfy the increasing demand of producing new types of MCC-based materials on an industrial scale. Therefore, this review assembles the current knowledge on the isolation of MCC from different sources using various procedures, its characterization, and its application in bio-composites. Challenges and future opportunities of MCC-based composites are discussed as well as obstacles remaining for their extensive uses.
    MeSH terms: Cellulose/isolation & purification*; Cellulose/chemistry*
  6. Fong SY, Piva T, Dekiwadia C, Urban S, Huynh T
    BMC Complement Altern Med, 2016 Sep 20;16:368.
    PMID: 27646974 DOI: 10.1186/s12906-016-1348-x
    Clinacanthus nutans (Burm. f.) Lindau leaves are widely used by cancer patients and the leaf extracts possess cytotoxic and antiproliferative effects on several human cancer cell lines. However, the effect of C. nutans leaf extract on human melanoma, which is the least common but most fatal form of skin cancer and one of the most common cancers diagnosed in both sexes worldwide, is unknown. There is also limited information on whether the bioactivity of extracts differs between C. nutans leaves grown in different geographical locations with varying environmental conditions.
    MeSH terms: Antineoplastic Agents/pharmacology*; Antineoplastic Agents/chemistry; Humans; Plant Extracts/toxicity*; Plant Extracts/chemistry; Thailand; Apoptosis/drug effects*; Plant Leaves/chemistry*; Acanthaceae/chemistry*; Cell Line, Tumor
  7. Hoo FK, Foo YL, Lim SM, Ching SM, Boo YL
    Pak J Med Sci, 2016 Jul-Aug;32(4):841-845.
    PMID: 27648025
    Acute coronary syndrome (ACS) is one of the leading cause of morbidity and mortality worldwide. It is relatively uncommon in young adults as compared to the older population. Our objective was to assess the prevalence, demographic distribution, and risk factors for acute coronary syndrome (ACS) in patients less than 45 years of age admitted to a Malaysian tertiary care centre.
    MeSH terms: Adult; Hospitalization; Humans; Morbidity; Risk Factors; Prevalence; Acute Coronary Syndrome; Tertiary Care Centers
  8. Sing DY, Boo YL, Mukhlis R, Chin PW, Hoo FK
    Pak J Med Sci, 2016 Jul-Aug;32(4):999-1004.
    PMID: 27648056
    Backgrounds & Objective: Antimicrobial resistance is an alarming public health threat that requires urgent global solution. Implementation of antimicrobial stewardship program (ASP) is an essential practice element for healthcare institutions in gate-keeping judicious antimicrobial use. This study highlighted the development, first year experience, and result of the implementation of ASP utilizing persuasive and restrictive approaches in a Malaysian district hospital.
    Methods: An observational study was conducted between January 2015 to December 2015 on implementation of ASP among hospitalized inpatients age 12 years old and above.
    Results: Recommendations were provided for 60% of cases (110 patients) with the average acceptance rate of 83.33%. Majority of the interventions were to stop the antimicrobial therapy (30.3%), and the most common audited antimicrobials was Piperacillin/Tazobactam (25.5%), followed by Meropenem (11.82%), Amoxicillin/Clavulanate and Vancomycin (8.18%) respectively. The concordance rate towards authorization policy was increased in 2015 (71.59% of cases) as compared before the implementation of ASP in 2014 (60.6% of cases). Restrictive enforcement under ASP had been shown to improve significantly adherence rate towards antimicrobials authorization policy (p-value: 0.004).
    Conclusion: ASP was successfully implemented in a district hospital. Future studies on its clinical outcomes are important to evaluate its effectiveness as well as focus on the improvement to the pre-existing strategies and measures.
    KEY WORDS: Antimicrobial stewardship, Anti-Infective agents, Drug resistance, Malaysia, Rural hospital
    MeSH terms: Anti-Infective Agents; Anti-Bacterial Agents; Child; Hospitals, District; Hospitals, Rural; Humans; Inpatients; Malaysia; Persuasive Communication; Public Health; Vancomycin; Amoxicillin-Potassium Clavulanate Combination; Drug Resistance, Bacterial
  9. Chong HC, Chai FY, Balakrishnan D, Asilah SM, Adila IN, Syibrah KZ
    Case Rep Surg, 2016;2016:6067374.
    PMID: 27648337
    Subhepatically located caecum and appendix is a very rare entity. It occurs due to the anomaly in fetal gut rotation that results in an incomplete rotation and fixation of the intestine. Appendicitis, which is a common surgical emergency, in combination with the abnormal subhepatic location, presents a great challenge in its diagnosis and management. Here, we describe a 42-year-old male with chronic dyspepsia who presented with sepsis and severe pain at his right hypochondriac and epigastric region. The final diagnosis was acute appendicitis of the subhepatic appendix. Our discussion focuses on the diagnostic approach and clinical and surgical management. We hope that our report will increase the awareness among the clinicians and hasten the management of such rare condition to avoid complications.
    MeSH terms: Acute Disease; Appendicitis; Appendix; Dyspepsia; Emergencies; Male; Pain; Rotation; Sepsis
  10. Loong SK, Soh YH, Mahfodz NH, Johari J, AbuBakar S
    Emerg Infect Dis, 2016 10;22(10):1834-5.
    PMID: 27648477 DOI: 10.3201/eid2210.151114
    MeSH terms: Adult; Arthroscopy; Biopsy; Humans; Male; RNA, Ribosomal, 16S/genetics; Synovitis/diagnosis*; Synovitis/microbiology*; Biomarkers; Sequence Analysis, DNA; Burkholderia Infections/diagnosis*; Burkholderia Infections/microbiology*; Arthroplasty, Replacement, Knee/adverse effects
  11. Aziz MY, Abu N, Yeap SK, Ho WY, Omar AR, Ismail NH, et al.
    Molecules, 2016 Sep 14;21(9).
    PMID: 27649120 DOI: 10.3390/molecules21091228
    Despite progressive research being done on drug therapy to treat breast cancer, the number of patients succumbing to the disease is still a major issue. Combinatorial treatment using different drugs and herbs to treat cancer patients is of major interest in scientists nowadays. Doxorubicin is one of the most used drugs to treat breast cancer patients. The combination of doxorubicin to other drugs such as tamoxifen has been reported. Nevertheless, the combination of doxorubicin with a natural product-derived agent has not been studied yet. Morinda citrifolia has always been sought out for its remarkable remedies. Damnacanthal, an anthraquinone that can be extracted from the roots of Morinda citrifolia is a promising compound that possesses a variety of biological properties. This study aimed to study the therapeutic effects of damnacanthal in combination with doxorubicin in breast cancer cells. Collectively, the combination of both these molecules enhanced the efficacy of induced cell death in MCF-7 as evidenced by the MTT assay, cell cycle, annexin V and expression of apoptosis-related genes and proteins. The effectiveness of doxorubicin as an anti-cancer drug was increased upon addition of damnacanthal. These results could provide a promising approach to treat breast cancer patients.
    MeSH terms: Anthraquinones/pharmacokinetics; Anthraquinones/chemistry; Antineoplastic Combined Chemotherapy Protocols/pharmacology*; Antineoplastic Combined Chemotherapy Protocols/chemistry; Breast Neoplasms/drug therapy*; Breast Neoplasms/metabolism; Doxorubicin/pharmacology; Doxorubicin/chemistry; Drug Screening Assays, Antitumor; Female; Humans; Morinda/chemistry; MCF-7 Cells
  12. Qazi HH, Mohammad AB, Ahmad H, Zulkifli MZ
    Sensors (Basel), 2016 Sep 15;16(9).
    PMID: 27649195 DOI: 10.3390/s16091505
    A D-shaped polarization-maintaining fiber (PMF) as fiber optic sensor for the simultaneous monitoring of strain and the surrounding temperature is presented. A mechanical end and edge polishing system with aluminum oxide polishing film is utilized to perform sequential polishing on one side (lengthwise) of the PMF in order to fabricate a D-shaped cross-section. Experimental results show that the proposed sensor has high sensitivity of 46 pm/µε and 130 pm/°C for strain and temperature, respectively, which is significantly higher than other recently reported work (mainly from 2013) related to fiber optic sensors. The easy fabrication method, high sensitivity, and good linearity make this sensing device applicable in various applications such as health monitoring and spatial analysis of engineering structures.
    MeSH terms: Aluminum Oxide; Dietary Fiber; Fiber Optic Technology; Temperature; Spatial Analysis
  13. Zahran MH, Fahmy O, El-Hefnawy AS, Ali-El-Dein B
    Climacteric, 2016 Dec;19(6):546-550.
    PMID: 27649461
    OBJECTIVES: To evaluate the impact of radical cystectomy and urinary diversion on female sexual function.

    MATERIALS AND METHODS: A Medline search was conducted according to the PRISMA statement for all English full-text articles published between 1980 and 2016 and assessing female sexual function post radical cystectomy and urinary diversion. Eligible studies were subjected to critical analysis and revision. The primary outcomes were the reporting methods for female sexual dysfunction (FSD), manifestations of FSD, and factors associated with FSD, postoperative recoverability of FSD, and awareness level regarding FSD.

    RESULTS: From the resulting 117 articles, 11 studies were finally included in our systematic review, with a total of 361 women. Loss of sexual desire and orgasm disorders were the most frequently reported (49% and 39%). Dyspareunia and vaginal lubrication disorders were reported in 25% and 9.5%, respectively. The incidence of sexual dysfunction was 10% in 30 patients receiving genital- or nerve-sparing cystectomy vs. 59% receiving conventional cystectomy.

    CONCLUSION: Although female sexual function is an important predictor of health-related quality of life post radical cystectomy and urinary diversion, the available literature is not enough to provide proper information for surgeons and patients.

    MeSH terms: Adult; Aged; Dyspareunia/epidemiology; Female; Humans; Middle Aged; Postoperative Complications/epidemiology*; Quality of Life; Sexual Dysfunction, Physiological/epidemiology*; Urinary Diversion/adverse effects*; Cystectomy/adverse effects*; MEDLINE; Sexual Dysfunctions, Psychological/epidemiology*
  14. Weiland F, Arentz G, Klingler-Hoffmann M, McCarthy P, Lokman NA, Kaur G, et al.
    J Proteome Res, 2016 11 04;15(11):4073-4081.
    PMID: 27569743
    Although acetylation is regarded as a common protein modification, a detailed proteome-wide profile of this post-translational modification may reveal important biological insight regarding differential acetylation of individual proteins. Here we optimized a novel peptide IEF fractionation method for use prior to LC-MS/MS analysis to obtain a more in depth coverage of N-terminally acetylated proteins from complex samples. Application of the method to the analysis of the serous ovarian cancer cell line OVCAR-5 identified 344 N-terminally acetylated proteins, 12 of which are previously unreported. The protein peptidyl-prolyl cis-trans isomerase A (PPIA) was detected in both the N-terminally acetylated and unmodified forms and was further analyzed by data-independent acquisition in carboplatin-responsive parental OVCAR-5 cells and carboplatin-resistant OVCAR-5 cells. This revealed a higher ratio of unacetylated to acetylated N-terminal PPIA in the parental compared with the carboplatin-resistant OVCAR-5 cells and a 4.1-fold increase in PPIA abundance overall in the parental cells relative to carboplatin-resistant OVCAR-5 cells (P = 0.015). In summary, the novel IEF peptide fractionation method presented here is robust, reproducible, and can be applied to the profiling of N-terminally acetylated proteins. All mass spectrometry data is available as a ProteomeXchange repository (PXD003547).
    MeSH terms: Acetylation*; Antineoplastic Agents/pharmacology; Female; Chemical Fractionation/methods; Humans; Ovarian Neoplasms/drug therapy*; Peptide Fragments/analysis*; Protein Processing, Post-Translational; Mass Spectrometry; Carboplatin/pharmacology; Drug Resistance, Neoplasm*; Databases, Protein; Proteomics/methods; Cell Line, Tumor
  15. Ghoussaini M, French JD, Michailidou K, Nord S, Beesley J, Canisus S, et al.
    Am J Hum Genet, 2016 Oct 06;99(4):903-911.
    PMID: 27640304 DOI: 10.1016/j.ajhg.2016.07.017
    Genome-wide association studies (GWASs) have revealed increased breast cancer risk associated with multiple genetic variants at 5p12. Here, we report the fine mapping of this locus using data from 104,660 subjects from 50 case-control studies in the Breast Cancer Association Consortium (BCAC). With data for 3,365 genotyped and imputed SNPs across a 1 Mb region (positions 44,394,495-45,364,167; NCBI build 37), we found evidence for at least three independent signals: the strongest signal, consisting of a single SNP rs10941679, was associated with risk of estrogen-receptor-positive (ER+) breast cancer (per-g allele OR ER+ = 1.15; 95% CI 1.13-1.18; p = 8.35 × 10-30). After adjustment for rs10941679, we detected signal 2, consisting of 38 SNPs more strongly associated with ER-negative (ER-) breast cancer (lead SNP rs6864776: per-a allele OR ER- = 1.10; 95% CI 1.05-1.14; p conditional = 1.44 × 10-12), and a single signal 3 SNP (rs200229088: per-t allele OR ER+ = 1.12; 95% CI 1.09-1.15; p conditional = 1.12 × 10-05). Expression quantitative trait locus analysis in normal breast tissues and breast tumors showed that the g (risk) allele of rs10941679 was associated with increased expression of FGF10 and MRPS30. Functional assays demonstrated that SNP rs10941679 maps to an enhancer element that physically interacts with the FGF10 and MRPS30 promoter regions in breast cancer cell lines. FGF10 is an oncogene that binds to FGFR2 and is overexpressed in ∼10% of human breast cancers, whereas MRPS30 plays a key role in apoptosis. These data suggest that the strongest signal of association at 5p12 is mediated through coordinated activation of FGF10 and MRPS30, two candidate genes for breast cancer pathogenesis.
    MeSH terms: Alleles; Breast Neoplasms/genetics*; Breast Neoplasms/metabolism*; Chromosomes, Human, Pair 5/genetics*; Enhancer Elements, Genetic/genetics; Haplotypes/genetics; Humans; Promoter Regions, Genetic/genetics; Receptors, Estrogen/metabolism*; Case-Control Studies; Genetic Predisposition to Disease/genetics*; Polymorphism, Single Nucleotide/genetics*; Quantitative Trait Loci/genetics; Cell Line, Tumor; Receptor, Fibroblast Growth Factor, Type 2/metabolism; Fibroblast Growth Factor 10/genetics*; Fibroblast Growth Factor 10/metabolism
  16. Yaakop AS, Chan KG, Ee R, Lim YL, Lee SK, Manan FA, et al.
    Sci Rep, 2016 09 19;6:33660.
    PMID: 27641516 DOI: 10.1038/srep33660
    Jeotgalibacillus malaysiensis, a moderate halophilic bacterium isolated from a pelagic area, can endure higher concentrations of sodium chloride (NaCl) than other Jeotgalibacillus type strains. In this study, we therefore chose to sequence and assemble the entire J. malaysiensis genome. This is the first report to provide a detailed analysis of the genomic features of J. malaysiensis, and to perform genetic comparisons between this microorganism and other halophiles. J. malaysiensis encodes a native megaplasmid (pJeoMA), which is greater than 600 kilobases in size, that is absent from other sequenced species of Jeotgalibacillus. Subsequently, RNA-Seq-based transcriptome analysis was utilised to examine adaptations of J. malaysiensis to osmotic stress. Specifically, the eggNOG (evolutionary genealogy of genes: Non-supervised Orthologous Groups) and KEGG (Kyoto Encyclopaedia of Genes and Genomes) databases were used to elucidate the overall effects of osmotic stress on the organism. Generally, saline stress significantly affected carbohydrate, energy, and amino acid metabolism, as well as fatty acid biosynthesis. Our findings also indicate that J. malaysiensis adopted a combination of approaches, including the uptake or synthesis of osmoprotectants, for surviving salt stress. Among these, proline synthesis appeared to be the preferred method for withstanding prolonged osmotic stress in J. malaysiensis.
    MeSH terms: Adaptation, Biological; Ion Channels/metabolism; Models, Biological; Osmotic Pressure*; Phylogeny; Plasmids/genetics; Stress, Physiological*; Cluster Analysis; Genome, Bacterial; Metabolic Networks and Pathways; Planococcaceae/classification; Planococcaceae/genetics*; Planococcaceae/metabolism*; Transcriptome*
  17. Almeida HF, Carvalho PJ, Kurnia KA, Lopes-da-Silva JA, Coutinho JA, Freire MG
    Fluid Phase Equilib, 2016 Feb 15;409:458-465.
    PMID: 27642224
    Ionic liquids (ILs) with cyano-functionalized anions are a set of fluids that are still poorly characterized despite their remarkably low viscosities and potential applications. Aiming at providing a comprehensive study on the influence of the number of -CN groups through the surface tension and surface organization of ILs, the surface tensions of imidazolium-based ILs with cyano-functionalized anions were determined at atmospheric pressure and in the (298.15 to 343.15) K temperature range. The ILs investigated are based on 1-alkyl-3-methylimidazolium cations (alkyl = ethyl, butyl and hexyl) combined with the [SCN]-, [N(CN)2]-, [C(CN)3]- and [B(CN)4]-anions. Although the well-known trend regarding the surface tension decrease with the increase of the size of the aliphatic moiety at the cation was observed, the order obtained for the anions is more intricate. For a common cation and at a given temperature, the surface tension decreases according to: [N(CN)2]- > [SCN]- > [C(CN)3]- > [B(CN)4]-. Therefore, the surface tension of this homologous series does not decrease with the increase of the number of -CN groups at the anion as has been previously shown by studies performed with a more limited matrix of ILs. A maximum in the surface tension and critical temperature was observed for [N(CN)2]-based ILs. Furthermore, a minimum in the surface entropy, indicative of a highly structured surface, was found for the same class of ILs. All these evidences seem to be a result of stronger hydrogen-bonding interactions occurring in [N(CN)2]-based ILs, when compared with the remaining CN-based counterparts, and as sustained by cation-anion interaction energies derived from the Conductor Like Screening Model for Real Solvents (COSMO-RS).
  18. Wali S, Gupta R, Yu JJ, Mfuh A, Gao X, Guentzel MN, et al.
    Metabolomics, 2016 Apr;12(4).
    PMID: 27642272
    INTRODUCTION: Chlamydia trachomatis (Ct), is the leading cause of sexually transmitted infections worldwide. Host transcriptomic- or proteomic profiling studies have identified key molecules involved in establishment of Ct infection or the generation of anti Ct-immunity. However, the contribution of the host metabolome is not known.

    OBJECTIVES: The objective of this study was to determine the contribution of host metabolites in genital Ct infection.

    METHODS: We used high-performance liquid chromatography-mass spectrometry, and mapped lipid profiles in genital swabs obtained from female guinea pigs at days 3, 9, 15, 30 and 65 post Ct serovar D intravaginal infection.

    RESULTS: Across all time points assessed, 13 distinct lipid species including choline, ethanolamine and glycerol were detected. Amongst these metabolites, phosphatidylcholine (PC) was the predominant phospholipid detected from animals actively shedding bacteria i.e., at 3, 9, and 15 days post infection. However, at days 30 and 65 when the animals had cleared the infection, PC was observed to be decreased compared to previous time points. Mass spectrometry analyses of PC produced in guinea pigs (in vivo) and 104C1 guinea pig cell line (in vitro) revealed distinct PC species following Ct D infection. Amongst these, PC 16:0/18:1 was significantly upregulated following Ct D infection (p < 0.05, >twofold change) in vivo and in vitro infection models investigated in this report. Exogenous addition of PC 16:0/18:1 resulted in significant increase in Ct D in Hela 229 cells.

    CONCLUSION: This study demonstrates a role for host metabolite, PC 16:0/18:1 in regulating genital Ct infection in vivo and in vitro.

    MeSH terms: Animals; Chlamydia Infections; Chlamydia trachomatis; Choline; Chromatography, High Pressure Liquid; Ethanolamines; Female; Genitalia; Glycerol; Guinea Pigs; HeLa Cells; Humans; Mass Spectrometry; Ethanolamine; Proteomics; Lecithins; Metabolome; Serogroup
  19. Mohammed NK, Abd Manap MY, Tan CP, Muhialdin BJ, Alhelli AM, Meor Hussin AS
    PMID: 27642353 DOI: 10.1155/2016/6273817
    The Nigella sativa L. popularly referred to as black seeds are widely used as a form of traditional nutrition and medicine. N. sativa seeds were used for the extraction of their oil by way of supercritical fluid extraction (SFE) and cold press (CP) to determine the physicochemical properties, antioxidant activity, and thermal behavior. The GC-MS results showed the primary constituents in the Nigella sativa oil (NSO) were Caryophyllene (17.47%) followed by thymoquinone (TQ) (11.80%), 1,4-Cyclohexadiene (7.17%), longifolene (3.5%), and carvacrol (1.82%). The concentration of TQ was found to be 6.63 mg/mL for oil extracted using SFE and 1.56 mg/mL for oil extracted by CP method. The antioxidant activity measured by DPPH and the IC50 was 1.58 mg/mL and 2.30 mg/mL for SFE oil and cold pressed oil, respectively. The ferric reducing/antioxidant power (FRAP) activity for SFE oil and CP oil was 538.67 mmol/100 mL and 329.00 mmol/100 mL, respectively. The total phenolic content (TPC) of SFE oil was 160.51 mg/100 mL and 94.40 mg/100 mL for CP oil presented as gallic acid equivalents (GAE). This research showed that a high level of natural antioxidants could be derived from NSO extracted by SFE.
    MeSH terms: Antioxidants; Gallic Acid; Gas Chromatography-Mass Spectrometry; Plant Oils; Sesquiterpenes; Benzoquinones; Inhibitory Concentration 50; Chromatography, Supercritical Fluid; Nigella sativa
  20. Chia YC
    J Hypertens, 2016 Sep;34 Suppl 1 - ISH 2016 Abstract Book:e16-e17.
    PMID: 27753834
    Conference abstract SY04-4: Many cardiovascular disease (CVD) risk prediction tools have been developed in an attempt to identify those at highest risk in order for them to benefit from interventional treatment. The first CVD risk tool that was developed was the coronary heart disease risk tool by the Framingham Heart Study in 1998 (1). However the Framingham Risk Score could overestimate (or underestimate) risk in populations other than the US population. Hence several other risk engines have also been developed, primarily for a better fit in the communities in which the tools are to be used (2, 3). Having said that the Framingham Heart Study risk tool has been validated in several populations (4, 5) and found to work reasonably well after some recalibration.Most risk prediction tools predict short term risk ie over a period of 10 years but since more recently risk tools now attempt to predict life-time risk or at least risk over the next 30 years. (6-8). The practical use of these risk prediction tools is that it is able to separate those at high risk (ie > 20% risk of a CVD event fatal or non-fatal event in the next 10 years) from those with the lowest risk (< 10% risk over 10 years). It then helps practitioners to triage them to either receive preventive therapy (high risk group) or none at all (low risk group) respectively. However in those with medium risk ie between 10-20%, the decision to offer treatment or not is less clear. In such a situation, other CVD risk factors for example family history of premature coronary heart disease, other biomarkers like elevated hs-CRP, presence of chronic kidney disease or albuminuria can be employed to further stratify risk.It is known that risk prediction tools are very much age dependent and in a younger individual with mildly raised CVD risk factors, his global CVD risk may be grossly under-estimated. Here additional CVD risk factors beyond those traditionally used in risk engines should be sought in order to recalibrate that individual's seemingly low risk and earlier intervention introduced if indeed he is of higher risk than what has been predicted by the conventional risk tools. Here too the use of life-time risk is probably of more importance than the traditional 10 year risk tool, again in order to identify those seemingly at "low" risk 10 year risk to receive treatment if the life-time risk is greater compared to an individual of the same age with optimal parameters. Furthermore while it is known that those with highest risk benefit the most from intervention, it is the population at large with the low or lower risk which contributes most to total CV morbidity and mortality in a country or community.Hence while short term risk prediction to identify those at highest risk is useful particularly in the presence of limited resources, attention should also be paid to those with short term low risk if the aim is to reduce CVD morbidity and mortality in any substantial way.
    MeSH terms: Albuminuria; Attention; Cardiovascular Diseases; Coronary Disease; Male; Morbidity; Risk Factors; Triage; Biomarkers; Renal Insufficiency, Chronic
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