Browse publications by year: 2016

  1. Danish-Daniel M, Han Ming G, Noor ME, Yeong YS, Usup G
    Genom Data, 2016 Dec;10:12-4.
    PMID: 27625991 DOI: 10.1016/j.gdata.2016.08.015
    Mameliella alba strain UMTAT08 was isolated from clonal culture of paralytic shellfish toxin producing dinoflagellate, Alexandrium tamiyavanichii. Genome of the strain UMTAT08 was sequenced in order to gain insights into the dinoflagellate-bacteria interactions. The draft genome sequence of strain UMTAT08 contains 5.84Mbp with an estimated G + C content of 65%, 5717 open reading frames, 5 rRNAs and 49 tRNAs. It contains genes related to nutrients uptake, quorum sensing and environmental tolerance related genes. Gene clusters for the biosynthesis of type 1 polyketide synthase, bacteriocin, microcin, terpene and ectoine were also identified. This is suggesting that the bacterium possesses diverse adaptation strategy to survive within the dinoflagellate phycosphere. The draft genome sequence and annotation have been deposited at DDBJ/EMBL/GenBank under the accession number JSUQ00000000.
  2. Wongrattanakamon P, Lee VS, Nimmanpipug P, Jiranusornkul S
    Data Brief, 2016 Dec;9:35-42.
    PMID: 27626051 DOI: 10.1016/j.dib.2016.08.004
    The data is obtained from exploring the modulatory activities of bioflavonoids on P-glycoprotein function by ligand-based approaches. Multivariate Linear-QSAR models for predicting the induced/inhibitory activities of the flavonoids were created. Molecular descriptors were initially used as independent variables and a dependent variable was expressed as pFAR. The variables were then used in MLR analysis by stepwise regression calculation to build the linear QSAR data. The entire dataset consisted of 23 bioflavonoids was used as a training set. Regarding the obtained MLR QSAR model, R of 0.963, R (2)=0.927, [Formula: see text], SEE=0.197, F=33.849 and q (2)=0.927 were achieved. The true predictabilities of QSAR model were justified by evaluation with the external dataset (Table 4). The pFARs of representative flavonoids were predicted by MLR QSAR modelling. The data showed that internal and external validations may generate the same conclusion.
    MeSH terms: Flavonoids; Ligands; Quantitative Structure-Activity Relationship; Flavones
  3. Abdullah NF, Teo PS, Foo LH
    Nutrients, 2016 Sep 12;8(9).
    PMID: 27626444 DOI: 10.3390/nu8090551
    OBJECTIVE: The aim of the study was to identify the ethnic differences in dietary patterns and its association with socio-economic, dietary and lifestyle practices among adolescents in Kelantan, Malaysia.

    METHODS: A population-based study of 454 adolescents aged 12 to 19 years was included. A validated food frequency questionnaire was used to assess dietary patterns and three dietary patterns were identified based on the principal component analysis method.

    RESULTS: Malay adolescents had significantly higher scores for the Western-based food pattern and local-based food pattern, whereas Chinese adolescents showed higher scores for the healthy-based food pattern. Multivariate analyses show that age and physical activity (PA) levels were positively associated with healthy-based food pattern in Malay (All, p < 0.001), whereas higher consumption of eating-out from home (EatOut) (p = 0.014) and fast food (p = 0.041) were negatively associated. High weekly breakfast skipping (p < 0.001) and EatOut (p = 0.003) were positively associated with a Western-based pattern, whereas age (p < 0.001) and household income (p = 0.005) were negatively associated. Higher frequency of daily snacking (p = 0.013) was positively associated with local-based food pattern. For Chinese adolescents, age (p < 0.001), PA levels (p < 0.001) and maternal education level (p = 0.035) showed positive associations with the healthy-based pattern, whereas high EatOut (p = 0.001) and fast food intakes (p = 0.001) were negatively associated. Higher weekly consumption of EatOut (p = 0.007), fast food (p = 0.023) and carbonated beverages (p = 0.023), and daily snacking practice (p = 0.004) were positively associated with higher Western-based food pattern, whereas age (p = 0.004) was inversely associated.

    CONCLUSION: This study showed that there were significant differences in dietary patterns and its association factors between Malay and Chinese adolescents. More importantly, these findings suggest that unhealthy dietary and lifestyle practices could increase the risk of adherence to unhealthy Western-based food pattern that is high in fat, sugar and salt contents, and, consequently, increase the risk of developing obesity and metabolic-related disorders during these critical years of growth.

    MeSH terms: Adolescent; Child; Diet Surveys; Eating/ethnology*; Eating/physiology*; Feeding Behavior/ethnology*; Feeding Behavior/physiology*; Female; Humans; Malaysia; Male; Socioeconomic Factors; Adolescent Nutritional Physiological Phenomena/ethnology*; Young Adult
  4. Saidi M, Sadeghifard N, Kazemian H, Sekawi Z, Badakhsh B, Friadian S, et al.
    Drug Res (Stuttg), 2016 Sep 14.
    PMID: 27626607
    Herbal medicines are defined as traditionally used natural products. The current study in an attempt try to investigate the antibacterial activity on extended spectrum beta-lactamases (ESBL), methalo beta-lactamases (MBL) producing gram negative bacteria and methicillin resistant Staphylococcus aureus (MRSA) and subsequently, to determine the antioxidant activity of Thymus daenensis. For this reasons, firstly cytotoxicity of T. daenensis was determined by MTT assay. Then, essential oil was subjected for antibacterial and antioxidant activity. Our results demonstrated that 15 mg/ml concentration of T. daenensis inhibited both P. aeruginosa producing MBL and E.coli producing ESBL, while this value was 25 mg/ml concentration for MRSA inhibition. The association between phenolic compound and antioxidant activity was found for the ABTS(•+) method (43.52%) in the lowest level, while, for FRAD and DPPH(•) methods the opposite story occurred (70.5% correlation for DPPH(•) and 50.9% for FRAD). Our findings suggested that T. daenensis has a potential herbal medicine that should be considered as an antibacterial and antioxidant with very low toxicity.
  5. Toh YF, Yew SM, Chan CL, Na SL, Lee KW, Hoh CC, et al.
    PLoS One, 2016;11(9):e0162095.
    PMID: 27626635 DOI: 10.1371/journal.pone.0162095
    Pyrenochaeta unguis-hominis is a rare human pathogen that causes infection in human skin and nail. P. unguis-hominis has received little attention, and thus, the basic biology and pathogenicity of this fungus is not fully understood. In this study, we performed in-depth analysis of the P. unguis-hominis UM 256 genome that was isolated from the skin scraping of a dermatitis patient. The isolate was identified to species level using a comprehensive multilocus phylogenetic analysis of the genus Pyrenochaeta. The assembled UM 256 genome has a size of 35.5 Mb and encodes 12,545 putative genes, and 0.34% of the assembled genome is predicted transposable elements. Its genomic features propose that the fungus is a heterothallic fungus that encodes a wide array of plant cell wall degrading enzymes, peptidases, and secondary metabolite biosynthetic enzymes. Antifungal drug resistance genes including MDR, CDR, and ERG11/CYP51 were identified in P. unguis-hominis UM 256, which may confer resistance to this fungus. The genome analysis of P. unguis-hominis provides an insight into molecular and genetic basis of the fungal lifestyles, understanding the unrevealed biology of antifungal resistance in this fungus.
    MeSH terms: Antifungal Agents/pharmacology; Ascomycota/drug effects; Ascomycota/genetics*; Ascomycota/metabolism; Dermatomycoses/microbiology*; DNA Transposable Elements/genetics; Genes, Fungal/genetics; Humans; Phylogeny; Skin/microbiology; Genome, Fungal/genetics*; Drug Resistance, Multiple, Fungal; Multilocus Sequence Typing
  6. Leong CR, Funami K, Oshiumi H, Mengao D, Takaki H, Matsumoto M, et al.
    Oncotarget, 2016 10 18;7(42):68179-68193.
    PMID: 27626689 DOI: 10.18632/oncotarget.11907
    Hepatitis B virus (HBV) barely induces host interferon (IFN)-stimulated genes (ISGs), which allows efficient HBV replication in the immortalized mouse hepatocytes as per human hepatocytes. Here we found that transfection of Isg20 plasmid robustly inhibits the HBV replication in HBV-infected hepatocytes irrespective of IRF3 or IFN promoter activation. Transfection of Isg20 is thus effective to eradicate HBV in the infected hepatocytes. Transfection of HBV genome or ε-stem of HBV pgRNA (active pgRNA moiety) failed to induce Isg20 in the hepatocytes, while control polyI:C (a viral dsRNA analogue mimic) activated MAVS pathway leading to production of type I IFN and then ISGsg20 via the IFN-α/β receptor (IFNAR). Consistently, addition of IFN-α induced Isg20 and partially suppressed HBV replication in hepatocytes. Chasing HBV RNA, DNA and proteins by blotting indicated that ISG20 expression decreased HBV RNA and replicative DNA in HBV-transfected cells, which resulted in low HBs antigen production and virus titer. The exonuclease domains of ISG20 mainly participated in HBV-RNA decay. In vivo hydrodynamic injection, ISG20 was crucial for suppressing HBV replication without degrading host RNA in the liver. Taken together, ISG20 acts as an innate anti-HBV effector that selectively degrades HBV RNA and blocks replication of infectious HBV particles. ISG20 would be a critical effector for ameliorating chronic HBV infection in the IFN therapy.
    MeSH terms: Animals; Antiviral Agents/pharmacology; Cell Line; Exonucleases/genetics*; Exonucleases/metabolism; Hepatitis B virus/genetics*; Hepatitis B virus/physiology; Humans; Liver/drug effects; Liver/metabolism; Liver/virology; Mice, Inbred C57BL; RNA, Viral/genetics*; RNA, Viral/metabolism; Virus Replication/drug effects; Virus Replication/genetics*; Interferon-alpha/pharmacology; Mice, Knockout; Hepatitis B, Chronic/genetics; Hepatitis B, Chronic/metabolism; Hepatitis B, Chronic/virology; Gene Expression Profiling/methods; RNA Stability; Hepatocytes/metabolism*; Hepatocytes/virology; Cell Line, Tumor; Receptor, Interferon alpha-beta/genetics; Receptor, Interferon alpha-beta/metabolism; Hep G2 Cells
  7. Setha T, Chantha N, Benjamin S, Socheat D
    PLoS Negl Trop Dis, 2016 09;10(9):e0004973.
    PMID: 27627758 DOI: 10.1371/journal.pntd.0004973
    A multi-phased study was conducted in Cambodia from 2005-2011 to measure the impact of larviciding with the bacterial larvicide, Bacillus thuringiensis israelensis (Bti), a water dispersible granule (WG) formulation on the vector, Aedes aegypti (L.) and the epidemiology. In our studies, all in-use containers were treated at 8 g/1000 L, including smaller containers and animal feeders which were found to contribute 23% of Ae aegypti pupae. The treated waters were subjected to routine water exchange activities. Pupal production was suppressed by an average 91% for 8 weeks. Pupal numbers continued to remain significantly lower than the untreated commune (UTC) for 13 weeks post treatment in the peak dengue vector season (p<0.05). Suppression of pupal production was supported by very low adult numbers in the treated commune. An average 70% of the household harbored 0-5 Ae aegypti mosquitoes per home for 8 weeks post treatment, but in the same period of time >50% of the household in the UTC harbored ≥11 mosquitoes per home. The adult population continued to remain at significantly much lower numbers in the Bti treated commune than in the UTC for 10-12 weeks post treatment (p<0.05). In 2011, a pilot operational program was evaluated in Kandal Province, a temephos resistant site. It was concluded that 2 cycles of Bti treatment in the 6 months monsoon season with complete coverage of the target districts achieved an overall dengue case reduction of 48% in the 6 treated districts compared to the previous year, 2010. Five untreated districts in the same province had an overwhelming increase of 352% of dengue cases during the same period of time. The larvicide efficacy, treatment of all in-use containers at the start of the monsoon season, together with treatment coverage of entire districts interrupted disease transmission in the temephos resistant province.
    MeSH terms: Temefos; Aedes/growth & development*; Animals; Bacillus thuringiensis/physiology*; Cambodia; Dengue/prevention & control*; Drug Resistance; Family Characteristics; Humans; Insect Vectors; Insecticides; Mosquito Control/methods*; Population Density; Pupa/growth & development; Surveys and Questionnaires; Seasons; Water Microbiology; Ecosystem
  8. Matin MM, Nath AR, Saad O, Bhuiyan MM, Kadir FA, Abd Hamid SB, et al.
    Int J Mol Sci, 2016 Aug 27;17(9).
    PMID: 27618893 DOI: 10.3390/ijms17091412
    Benzyl α-l-rhamnopyranoside 4, obtained by both conventional and microwave assisted glycosidation techniques, was subjected to 2,3-O-isopropylidene protection to yield compound 5 which on benzoylation and subsequent deprotection of isopropylidene group gave the desired 4-O-benzoylrhamnopyranoside 7 in reasonable yield. Di-O-acetyl derivative of benzoate 7 was prepared to get newer rhamnopyranoside. The structure activity relationship (SAR) of the designed compounds was performed along with the prediction of activity spectra for substances (PASS) training set. Experimental studies based on antimicrobial activities verified the predictions obtained by the PASS software. Protected rhamnopyranosides 5 and 6 exhibited slight distortion from regular ¹C₄ conformation, probably due to the fusion of pyranose and isopropylidene ring. Synthesized rhamnopyranosides 4-8 were employed as test chemicals for in vitro antimicrobial evaluation against eight human pathogenic bacteria and two fungi. Antimicrobial and SAR study showed that the rhamnopyranosides were prone against fungal organisms as compared to that of the bacterial pathogens. Interestingly, PASS prediction of the rhamnopyranoside derivatives 4-8 were 0.49 < Pa < 0.60 (where Pa is probability 'to be active') as antibacterial and 0.65 < Pa < 0.73 as antifungal activities, which showed significant agreement with experimental data, suggesting rhamnopyranoside derivatives 4-8 were more active against pathogenic fungi as compared to human pathogenic bacteria thus, there is a more than 50% chance that the rhamnopyranoside derivative structures 4-8 have not been reported with antimicrobial activity, making it a possible valuable lead compound.
    MeSH terms: Anti-Infective Agents/pharmacology*; Anti-Infective Agents/chemistry*; Bacteria/drug effects; Carbohydrate Sequence; Fungi/drug effects; Microbial Sensitivity Tests; Oligosaccharides/chemistry*; Rhamnose/chemistry*; Software; Structure-Activity Relationship
  9. Mohamed Yusoff A, Tan TK, Hari R, Koepfli KP, Wee WY, Antunes A, et al.
    Sci Rep, 2016 09 13;6:28199.
    PMID: 27618997 DOI: 10.1038/srep28199
    Pangolins are scale-covered mammals, containing eight endangered species. Maintaining pangolins in captivity is a significant challenge, in part because little is known about their genetics. Here we provide the first large-scale sequencing of the critically endangered Manis javanica transcriptomes from eight different organs using Illumina HiSeq technology, yielding ~75 Giga bases and 89,754 unigenes. We found some unigenes involved in the insect hormone biosynthesis pathway and also 747 lipids metabolism-related unigenes that may be insightful to understand the lipid metabolism system in pangolins. Comparative analysis between M. javanica and other mammals revealed many pangolin-specific genes significantly over-represented in stress-related processes, cell proliferation and external stimulus, probably reflecting the traits and adaptations of the analyzed pregnant female M. javanica. Our study provides an invaluable resource for future functional works that may be highly relevant for the conservation of pangolins.
    MeSH terms: Adaptation, Biological; Animals; Female; Species Specificity; Sequence Analysis, DNA/methods*; Sequence Analysis, RNA/methods*; Gene Expression Profiling/methods*; Metabolic Networks and Pathways; Endangered Species; High-Throughput Nucleotide Sequencing/methods*; Gene Ontology
  10. Yew CC, Alam MK, Rahman SA
    PMID: 27619027 DOI: 10.1016/j.ijporl.2016.07.026
    OBJECTIVES: This study is to evaluate the dental arch relationship and palatal morphology of unilateral cleft lip and palate patients by using EUROCRAN index, and to assess the factors that affect them using multivariate statistical analysis.

    METHOD: A total of one hundred and seven patients from age five to twelve years old with non-syndromic unilateral cleft lip and palate were included in the study. These patients have received cheiloplasty and one stage palatoplasty surgery but yet to receive alveolar bone grafting procedure. Five assessors trained in the use of the EUROCRAN index underwent calibration exercise and ranked the dental arch relationships and palatal morphology of the patients' study models. For intra-rater agreement, the examiners scored the models twice, with two weeks interval in between sessions. Variable factors of the patients were collected and they included gender, site, type and, family history of unilateral cleft lip and palate; absence of lateral incisor on cleft side, cheiloplasty and palatoplasty technique used. Associations between various factors and dental arch relationships were assessed using logistic regression analysis.

    RESULT: Dental arch relationship among unilateral cleft lip and palate in local population had relatively worse scoring than other parts of the world. Crude logistics regression analysis did not demonstrate any significant associations among the various socio-demographic factors, cheiloplasty and palatoplasty techniques used with the dental arch relationship outcome.

    CONCLUSIONS: This study has limitations that might have affected the results, example: having multiple operators performing the surgeries and the inability to access the influence of underlying genetic predisposed cranio-facial variability. These may have substantial influence on the treatment outcome. The factors that can affect unilateral cleft lip and palate treatment outcome is multifactorial in nature and remained controversial in general.

    MeSH terms: Child; Child, Preschool; Cleft Lip/surgery*; Cleft Palate/surgery*; Dental Arch*; Female; Humans; Male; Retrospective Studies; Multivariate Analysis; Logistic Models; Bone Transplantation; Treatment Outcome; Reconstructive Surgical Procedures
  11. Yadav P, Sarkale P, Patil D, Shete A, Kokate P, Kumar V, et al.
    Infect Genet Evol, 2016 11;45:224-229.
    PMID: 27619056 DOI: 10.1016/j.meegid.2016.09.010
    Bat-borne viral diseases are a major public health concern among newly emerging infectious diseases which includes severe acute respiratory syndrome, Nipah, Marburg and Ebola virus disease. During the survey for Nipah virus among bats at North-East region of India; Tioman virus (TioV), a new member of the Paramyxoviridae family was isolated from tissues of Pteropus giganteus bats for the first time in India. This isolate was identified and confirmed by RT-PCR, sequence analysis and electron microscopy. A range of vertebrate cell lines were shown to be susceptible to Tioman virus. Negative electron microscopy study revealed the "herringbone" morphology of the nucleocapsid filaments and enveloped particles with distinct envelope projections a characteristic of the Paramyxoviridae family. Sequence analysis of Nucleocapsid gene of TioV demonstrated sequence identity of 99.87% and 99.99% nucleotide and amino acid respectively with of TioV strain isolated in Malaysia, 2001. This report demonstrates the first isolation of Tioman virus from a region where Nipah virus activity has been noticed in the past and recent years. Bat-borne viruses have become serious concern world-wide. A Survey of bats for novel viruses in this region would help in recognizing emerging viruses and combating diseases caused by them.
    MeSH terms: Animals; Cell Line; Chick Embryo; Chiroptera/virology*; India
  12. Venugopalan SK, T S S, V N, S M M, S R
    Biomed Pharmacother, 2016 Oct;83:1485-1492.
    PMID: 27619103 DOI: 10.1016/j.biopha.2016.08.068
    Thymus mitochondria play a crucial role in immune function. This study identifies the novel protective role of N-Acetylglucosamine (NAG) in dexamethasone (DEX) induced mitochondrial perturbations in mice thymus. Mice were induced with DEX (5mg/kg) and treated with NAG i.p. (266μg/kg, 400μg/kg and 800μg/kg) for 14 days, Withanolide A (800μg/kg) has been used as positive control. Dose dependent treatment of NAG against DEX significantly restored the mitochondrial enzyme levels (ICDH, KDH, SDH and MDH) and elevated the mitochondrial glutathione antioxidants defense (GSH, SOD, GPX and GST) thus improving the ATP status which was confirmed by ultrastructural alterations in mitochondria and nucleus using TEM studies. Further histopathological studies also revealed that NAG attenuate DEX induced thymotoxicity. Finally, the study concludes that dose dependent treatment of NAG supports a potential role in preventing DEX induced thymotoxicity and NAG acts as a beneficial pharmacological intervention in the DEX induced thymic repercussions.
    MeSH terms: Acetylglucosamine/physiology*; Animals; Anti-Inflammatory Agents/pharmacology; Dexamethasone/pharmacology*; Dose-Response Relationship, Drug; Male; Mitochondria/drug effects*; Mitochondria/metabolism*; Thymus Gland/drug effects*; Thymus Gland/metabolism*; Mice
  13. Salih NF, Jafri ZM, Jaafar MS
    Environ Sci Pollut Res Int, 2016 Dec;23(23):23662-23674.
    PMID: 27619370
    This study was carried out to determine the concentration of (222)Rn, (226)Ra, and (238)U in 25 different toothpastes available in the local market in Penang, Malaysia, using a CR-39 detector. The results showed the maximum concentration of radon/ radium/uranium to be 4197.644 Bq.m(-3), 54.369 Bq.Kgm(-1), and 0.044 ppm in Colgate4; the annual effective dose was found (0.402 mSvy(-1)) in S07. The average concentration of radon (42 %, 3.224 KBq.m(-3)) was higher than the concentration of (214)Po, (218)Po in POS (32 %, 2.415 KBq.m(-3)) and POW (26 %, 1.979 KBq.m(-3)). Also the values of pH of samples ranged from 4.21 (highly acidic) in S04 to 9.97 (highly basic) in S07, with an average of 6.33 which tended towards an acidic behavior; a low or high pH for a long period of time can cause harmful side-effects and enamel erosion. Concentrations of heavy metals varied from the maximum value 56.156 ppm in the Ca elements in the Colgate 4 sample to a minimum value of -0.858 ppm in the Cd elements in Colgate 6 (Ca 56.156 ppm > Cd 51.572 ppm > Zn 41.039 ppm > Mg 11.682 ppm > Pb 11.009 ppm]. Monitoring the accumulation of these metals in toothpaste samples is very important: the average annual effective dose (0.3118 mSvy(-1)) was below the range (3-10 mSvy(-1)) reported by ICRP (1993), and therefore there is no evidence of health problems. Significant strong positive correlations were found (r = 1, Pearson correlation, p 
    MeSH terms: Environmental Monitoring*; Hydrogen-Ion Concentration; Malaysia; Radioactivity; Radioisotopes/analysis*; Soil Pollutants, Radioactive/analysis*; Toothpastes/chemistry*; Metals, Heavy/analysis*
  14. Jeevanaraj P, Hashim Z, Elias SM, Aris AZ
    Environ Sci Pollut Res Int, 2016 Dec;23(23):23714-23729.
    PMID: 27619374
    We identified marine fish species most preferred by women at reproductive age in Selangor, Malaysia, mercury concentrations in the fish muscles, factors predicting mercury accumulation and the potential health risk. Nineteen most preferred marine fish species were purchased (n = 175) from selected fisherman's and wholesale market. Length, weight, habitat, feeding habit and trophic level were recognised. Edible muscles were filleted, dried at 80 °C, ground on an agate mortar and digested in Multiwave 3000 using HNO3 and H2O2. Total mercury was quantified using VP90 cold vapour system with N2 carrier gas. Certified reference material DORM-4 was used to validate the results. Fish species were classified as demersal (7) and pelagic (12) or predators (11), zoo benthos (6) and planktivorous (2). Length, weight and trophic level ranged from 10.5 to 75.0 cm, 0.01 to 2.50 kg and 2.5 to 4.5, respectively. Geometric mean of total mercury ranged from 0.21 to 0.50 mg/kg; maximum in golden snapper (0.90 mg/kg). Only 9 % of the samples exceeded the JECFA recommendation. Multiple linear regression found demersal, high trophic (≥4.0) and heavier fishes to accumulate more mercury in muscles (R (2) = 27.3 %), controlling for all other factors. About 47 % of the fish samples contributed to mercury intake above the provisional tolerable level (45 μg/day). While only a small portion exceeded the JECFA fish Hg guideline, the concentration reported may be alarming for heavy consumers. Attention should be given in risk management to avoid demersal and high trophic fish, predominantly heavier ones.
    MeSH terms: Adolescent; Adult; Animals; Female; Fishes/metabolism*; Food Contamination/analysis*; Humans; Malaysia; Mercury/metabolism*; Water Pollutants, Chemical/metabolism*; Seafood/analysis*; Risk Assessment; Young Adult
  15. Akram Z, Abduljabbar T, Sauro S, Daood U
    Photodiagnosis Photodyn Ther, 2016 Dec;16:142-153.
    PMID: 27619532 DOI: 10.1016/j.pdpdt.2016.09.004
    BACKGROUND: To assess the efficacy of antimicrobial photodynamic therapy (aPDT) or laser therapy (LT) alone as an adjunct to scaling and root planing (SRP) on the gingival crevicular fluid (GCF) inflammatory proteins in periodontal disease.

    METHODS: Databases (MEDLINE via PubMed; EMBASE; Cochrane Central Register of Controlled Trials and Cochrane Oral Health Group Trials Register databases) were searched from 1980 up to and including July 2016. The addressed PICO question was: "What effect does aPDT and/or LT as an adjunct to SRP have on the GCF inflammatory proteins in periodontal disease patients?"

    RESULTS: Eight studies used aPDT while 10 studies used laser alone. Eight cytokines including tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-8, IL-10, interferon gamma (IFN-γ), matrix metalloproteinase (MMP)-8 and granulocyte colony-stimulating factor (GM-CSF) were eligible for qualitative analysis for aPDT and LT studies. Four aPDT studies showed significant reduction in IL-1β while one study showed significant reduction in TNF-α levels after aPDT application at follow-up. One study showed significant reduction of IFN-γ, IL-8 and GM-CSF levels after aPDT at follow-up. IL-1β significantly reduced in 4 LT studies, while one study showed significant decrease for IL-6 and TIMP-1 levels. MMP-8 and TNF-α showed significant reduction in three and one study respectively.

    CONCLUSION: It remains debatable whether adjunctive aPDT or LT is effective in the reduction of GCF inflammatory proteins in periodontal disease due to non-standard laser parameters and short follow up period. These findings should be considered preliminary and further studies with long-term follow up and standardized laser parameters are recommended.

    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Combined Modality Therapy/methods; Gingival Crevicular Fluid/immunology*; Humans; Middle Aged; Periodontitis/immunology*; Periodontitis/therapy*; Photochemotherapy/methods*; Dental Scaling/methods; Cytokines/immunology*; Root Planing/methods*; Treatment Outcome; Photosensitizing Agents/administration & dosage; Laser Therapy/methods*; Young Adult
  16. Kato M, Azimi MD, Fayaz SH, Shah MD, Hoque MZ, Hamajima N, et al.
    Chemosphere, 2016 Dec;165:27-32.
    PMID: 27619645 DOI: 10.1016/j.chemosphere.2016.08.124
    Toxic elements in drinking water have great effects on human health. However, there is very limited information about toxic elements in drinking water in Afghanistan. In this study, levels of 10 elements (chromium, nickel, copper, arsenic, cadmium, antimony, barium, mercury, lead and uranium) in 227 well drinking water samples in Kabul, Afghanistan were examined for the first time. Chromium (in 0.9% of the 227 samples), arsenic (7.0%) and uranium (19.4%) exceeded the values in WHO health-based guidelines for drinking-water quality. Maximum chromium, arsenic and uranium levels in the water samples were 1.3-, 10.4- and 17.2-fold higher than the values in the guidelines, respectively. We next focused on uranium, which is the most seriously polluted element among the 10 elements. Mean ± SD (138.0 ± 1.4) of the (238)U/(235)U isotopic ratio in the water samples was in the range of previously reported ratios for natural source uranium. We then examined the effect of our originally developed magnesium (Mg)-iron (Fe)-based hydrotalcite-like compounds (MF-HT) on adsorption for uranium. All of the uranium-polluted well water samples from Kabul (mean ± SD = 190.4 ± 113.9 μg/L; n = 11) could be remediated up to 1.2 ± 1.7 μg/L by 1% weight of our MF-HT within 60 s at very low cost (<0.001 cents/day/family) in theory. Thus, we demonstrated not only elevated levels of some toxic elements including natural source uranium but also an effective depurative for uranium in well drinking water from Kabul. Since our depurative is effective for remediation of arsenic as shown in our previous studies, its practical use in Kabul may be encouraged.
    MeSH terms: Afghanistan; Aluminum Hydroxide/pharmacology; Aluminum Hydroxide/chemistry*; Arsenic/analysis; Cadmium/analysis; Environmental Monitoring; Humans; Iron/pharmacology; Iron/chemistry*; Magnesium/pharmacology; Magnesium/chemistry*; Magnesium Hydroxide/pharmacology; Magnesium Hydroxide/chemistry*; Pakistan; Uranium/analysis*; Uranium/isolation & purification; Uranium/metabolism; Water Pollutants, Chemical/analysis*; Water Pollutants, Chemical/isolation & purification; Water Pollutants, Chemical/metabolism; Water Supply; Water Purification/economics; Water Purification/methods; Environmental Restoration and Remediation/economics; Environmental Restoration and Remediation/methods*; Water Quality; Drinking Water/analysis*; Water Wells
  17. Chaubal TV, Bapat RA, Patil PG, Shetty A
    Contact Derm., 2016 Oct;75(4):256-7.
    PMID: 27620128 DOI: 10.1111/cod.12625
    MeSH terms: Hand Dermatoses/diagnosis; Hand Dermatoses/etiology*; Humans; Male; Dermatitis, Occupational/diagnosis; Dermatitis, Occupational/etiology*; Skin Tests; Students, Dental*; Dermatitis, Allergic Contact/diagnosis; Dermatitis, Allergic Contact/etiology*; Gloves, Protective/adverse effects*; Latex Hypersensitivity/complications*; Latex Hypersensitivity/diagnosis; Young Adult
  18. Puttarak P, Sawangjit R, Chaiyakunapruk N
    J Ethnopharmacol, 2016 Dec 24;194:316-323.
    PMID: 27620659 DOI: 10.1016/j.jep.2016.09.021
    ETHNOPHARMACOLOGICAL RELEVANCE: Derris scandens (Roxb.) Benth. has been used as active ingredient in Thai traditional medicine recipes for pain treatment. Dry stem powder and ethanolic extract also recommended in Thailand National List of Essential Medicines (NLEMs) for musculoskeletal pain treatment as herbal medicine. However, no summarization of clinical effect and safety has been evaluated.

    OBJECTIVE: Our study aimed to determine the clinical effects and safety of D. scandens for musculoskeletal pain treatment compared with standard regimen, nonsteroidal anti-inflammatory drugs (NSAIDs).

    METHODS: International and Thai databases were searched from inception through August 2015. Comparative randomized controlled trials investigating oral D. scandens for musculoskeletal pain were included. Outcomes of interest included level of pain and adverse event. Mean changes of the outcomes from baseline were compared between D. scandens and NSAIDs by calculating mean difference.

    RESULTS: From 42 articles identified, 4 studies involving a total of 414 patients were included for efficacy analysis. The effects of oral D. scandens on reducing pain score were no different from those of non-steroidal anti-inflammatory drugs at any time points (3, 7, 14 days and overall). The overall pain reduction in the D. scandens group was not inferior to treatment with NSAIDs (weighted mean difference 0.06; 95% CI: -0.20, 0.31) without evident of heterogeneity (I(2)=0.00%, p=0.768). When compared, the adverse events (AEs) of D. scandens showed no different relative risk with NSAIDs. The major adverse events were gastrointestinal symptoms.

    CONCLUSION: D. scandens may be considered as an alternative for musculoskeletal pain reduction.

    MeSH terms: Humans; Plant Extracts/adverse effects; Plant Extracts/therapeutic use*; Thailand; Randomized Controlled Trials as Topic*; Derris/chemistry*; Musculoskeletal Pain/drug therapy*
  19. Jayaprakash PT, Hadi H, Srinivasan A, Chellamuthu P, Abd Aziz Mohd Yusop R, Hashim N
    J Forensic Sci, 2016 09;61(5):1390-3.
    PMID: 27621069 DOI: 10.1111/1556-4029.13187
    MeSH terms: Photography; Skull/anatomy & histology*; Reproducibility of Results; Forensic Anthropology*
  20. Abdullah AF, Chang KH
    J Forensic Sci, 2016 09;61(5):1411-2.
    PMID: 27621078 DOI: 10.1111/1556-4029.13166
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