Browse publications by year: 2016

  1. Pinnagoda K, Larsson HM, Vythilingam G, Vardar E, Engelhardt EM, Thambidorai RC, et al.
    Acta Biomater, 2016 10 01;43:208-217.
    PMID: 27450527 DOI: 10.1016/j.actbio.2016.07.033
    The treatment of congenital malformations or injuries of the urethra using existing autologous tissues can be associated with post-operative complications. Using rat-tail collagen, we have engineered an acellular high-density collagen tube. These tubes were made of 2 layers and they could sustain greater burst pressures than the monolayered tubes. Although it remains a weak material this 2 layered tube could be sutured to the native urethra. In 20 male New Zealand white rabbits, 2cm long grafts were sutured in place after subtotal excision of the urethra. This long-term study was performed in Lausanne (Switzerland) and in Kuala Lumpur (Malaysia). No catheter was placed post-operatively. All rabbits survived the surgical implantation. The animals were evaluated at 1, 3, 6, and 9months by contrast voiding cysto-urethrography, histological examination and immunohistochemistry. Spontaneous re-population of urothelial and smooth muscle cells on all grafts was demonstrated. Cellular organization increased with time, however, 20% of both fistula and stenosis could be observed post-operatively. This off-the shelf scaffold with a promising urethral regeneration has a potential for clinical application.

    STATEMENT OF SIGNIFICANCE: In this study we have tissue engineered a novel cell free tubular collagen based scaffold and used it as a urethral graft in a rabbit model. The novelty of our technique is that the tube can be sutured. Testing showed better burst pressures and the grafts could then be successfully implanted after a urethral excision. This long term study demonstrated excellent biocompatibility of the 2cm graft and gradual regeneration with time, challenging the current literature. Finally, the main impact is that we describe an off-the-shelf and cost-effective product with comparable surgical outcome to the cellular grafts.

    MeSH terms: Animals; Collagen/pharmacology*; Immunohistochemistry; Male; Materials Testing; Rabbits; Regeneration/drug effects*; Urethra/drug effects; Urethra/pathology; Urethra/physiology*; Urethra/surgery; Implants, Experimental; Tissue Engineering/methods*; Rats
  2. Lee YM, Sheppard E
    Accid Anal Prev, 2016 Oct;95(Pt A):202-8.
    PMID: 27450792 DOI: 10.1016/j.aap.2016.07.011
    Failure in making the correct judgment about the intention of an approaching vehicle at a junction could lead to a collision. This paper investigated the impact of dynamic information on drivers' judgments about the intentions of approaching cars and motorcycles, and whether a valid or invalid signal was provided was also manipulated. Participants were presented with videoclips of vehicles approaching a junction which terminated immediately before the vehicle made any manoeuvre, or images of the final frame of each video. They were asked to judge whether or not the vehicle would turn. Drivers were better in judging the manoeuvre of approaching vehicles in dynamic than static stimuli, for both vehicle types. Drivers were better in judging the manoeuvre of cars than motorcycles for videos, but not for photographs. Drivers were also better in judging the manoeuvre of approaching vehicles when a valid signal was provided than an invalid signal, demonstrating the importance of providing a valid signal while driving. However, drivers were still somewhat successful in their judgments in most of the conditions with an invalid signal, suggesting that drivers were able to focus on other cues to intention. Finally, given that dynamic stimuli more closely reflect the demands of real-life driving there may be a need for drivers to adopt a more cautious approach while inferring a motorcyclist's intentions.
    MeSH terms: Accidents, Traffic/prevention & control*; Accidents, Traffic/psychology*; Adolescent; Adult; Automobile Driving/psychology*; Automobiles; Female; Humans; Judgment; Lighting/instrumentation*; Malaysia; Male; Motion Perception*; Motorcycles; Visual Perception*; Intention*; Young Adult
  3. Karami A, Romano N, Galloway T, Hamzah H
    Environ Res, 2016 Nov;151:58-70.
    PMID: 27451000 DOI: 10.1016/j.envres.2016.07.024
    Despite the ubiquity of microplastics (MPs) in aquatic environments and their proven ability to carry a wide variety of chemicals, very little is known about the impacts of virgin or contaminant-loaded MPs on organisms. The primary aim of this study was to investigate the impacts of virgin or phenanthrene (Phe)-loaded low-density polyethylene (LDPE) fragments on a suite of biomarker responses in juvenile African catfish (Clarias gariepinus). Virgin LDPE (50 or 500µg/L) were preloaded with one of two nominal Phe concentrations (10 or 100µg/L) and were exposed to the fish for 96h. Our findings showed one or both Phe treatments significantly increased the degree of tissue change (DTC) in the liver while decreased the transcription levels of forkhead box L2 (foxl2) and tryptophan hydroxylase2 (tph2) in the brain of C. gariepinus. Exposure to either levels of virgin MPs increased the DTC in the liver and plasma albumin: globulin ratio while decreased the transcription levels of tph2. Moreover, MPs modulated (interacted with) the impact of Phe on the DTC in the gill, plasma concentrations of cholesterol, high-density lipoprotein (HDL), total protein (TP), albumin, and globulin, and the transcription levels of fushi tarazu-factor 1 (ftz-f1), gonadotropin-releasing hormone (GnRH), 11 β-hydroxysteroid dehydrogenase type 2 (11β-hsd2), and liver glycogen stores. Results of this study highlight the ability of virgin LDPE fragments to cause toxicity and to modulate the adverse impacts of Phe in C. gariepinus. Due to the wide distribution of MPs and other classes of contaminants in aquatic environments, further studies are urgently needed to elucidate the toxicity of virgin or contaminant-loaded MPs on organisms.
    MeSH terms: Animals; Catfishes*; Gills/drug effects; Gills/pathology; Liver/drug effects; Liver/pathology; Phenanthrenes/analysis; Phenanthrenes/toxicity*; Phthalic Acids/analysis; Polychlorinated Biphenyls/analysis; Water Pollutants, Chemical/analysis; Water Pollutants, Chemical/toxicity*; Biomarkers; Metals, Heavy/analysis; Polyethylene/toxicity*
  4. Lau S, Ng KH, Abdul Aziz YF
    Br J Radiol, 2016 Oct;89(1066):20160258.
    PMID: 27452264 DOI: 10.1259/bjr.20160258
    OBJECTIVE: To investigate the sensitivity and robustness of a volumetric breast density (VBD) measurement system to errors in the imaging physics parameters including compressed breast thickness (CBT), tube voltage (kVp), filter thickness, tube current-exposure time product (mAs), detector gain, detector offset and image noise.

    METHODS: 3317 raw digital mammograms were processed with Volpara(®) (Matakina Technology Ltd, Wellington, New Zealand) to obtain fibroglandular tissue volume (FGV), breast volume (BV) and VBD. Errors in parameters including CBT, kVp, filter thickness and mAs were simulated by varying them in the Digital Imaging and Communications in Medicine (DICOM) tags of the images up to ±10% of the original values. Errors in detector gain and offset were simulated by varying them in the Volpara configuration file up to ±10% from their default values. For image noise, Gaussian noise was generated and introduced into the original images.

    RESULTS: Errors in filter thickness, mAs, detector gain and offset had limited effects on FGV, BV and VBD. Significant effects in VBD were observed when CBT, kVp, detector offset and image noise were varied (p 

    MeSH terms: Female; Humans; Mammography/methods*; Physics; Quality Control; Radiographic Image Interpretation, Computer-Assisted; Retrospective Studies; Sensitivity and Specificity; Breast Density*
  5. Sulaiman I, Lim JC, Soo HL, Stanslas J
    Pulm Pharmacol Ther, 2016 Oct;40:52-68.
    PMID: 27453494 DOI: 10.1016/j.pupt.2016.07.005
    Extensive research into the therapeutics of asthma has yielded numerous effective interventions over the past few decades. However, adverse effects and ineffectiveness of most of these medications especially in the management of steroid resistant severe asthma necessitate the development of better medications. Numerous drug targets with inherent airway smooth muscle tone modulatory role have been identified for asthma therapy. This article reviews the latest understanding of underlying molecular aetiology of asthma towards design and development of better antiasthma drugs. New drug candidates with their putative targets that have shown promising results in the preclinical and/or clinical trials are summarised. Examples of these interventions include restoration of Th1/Th2 balance by the use of newly developed immunomodulators such as toll-like receptor-9 activators (CYT003-QbG10 and QAX-935). Clinical trials revealed the safety and effectiveness of chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) antagonists such as OC0000459, BI-671800 and ARRY-502 in the restoration of Th1/Th2 balance. Regulation of cytokine activity by the use of newly developed biologics such as benralizumab, reslizumab, mepolizumab, lebrikizumab, tralokinumab, dupilumab and brodalumab are at the stage of clinical development. Transcription factors are potential targets for asthma therapy, for example SB010, a GATA-3 DNAzyme is at its early stage of clinical trial. Other candidates such as inhibitors of Rho kinases (Fasudil and Y-27632), phosphodiesterase inhibitors (GSK256066, CHF 6001, roflumilast, RPL 554) and proteinase of activated receptor-2 (ENMD-1068) are also discussed. Preclinical results of blockade of calcium sensing receptor by the use of calcilytics such as calcitriol abrogates cardinal signs of asthma. Nevertheless, successful translation of promising preclinical data into clinically viable interventions remains a major challenge to the development of novel anti-asthmatics.
    MeSH terms: Animals; Asthma/drug therapy*; Asthma/physiopathology; Humans; Immunologic Factors/adverse effects; Immunologic Factors/pharmacology; Immunologic Factors/therapeutic use; Drug Design*; Anti-Asthmatic Agents/adverse effects; Anti-Asthmatic Agents/pharmacology; Anti-Asthmatic Agents/therapeutic use*; Translational Medical Research; Th1-Th2 Balance/drug effects; Molecular Targeted Therapy
  6. Azilawati MI, Dzulkifly MH, Jamilah B, Shuhaimi M, Amin I
    J Pharm Biomed Anal, 2016 Sep 10;129:389-397.
    PMID: 27454091 DOI: 10.1016/j.jpba.2016.07.012
    A detailed procedure for estimating uncertainty according to the Laboratory of Government Chemists/Valid Analytical Measurement (LGC/VAM) protocol for determination of 18 amino acids in gelatin is proposed. The expanded uncertainty was estimated using mainly the method validation data (precision and trueness). Other sources of uncertainties were contributed by components in standard preparation measurements. The method scope covered a single matrix (gelatin) under a wide range of analyte concentrations. The uncertainty of method precision, μ(P) was 0.0237-0.1128pmolμl(-1) in which hydroxyproline and histidine represented the lowest and highest values of uncertainties, respectively. Proline and phenylalanine represented the lowest and highest uncertainties value for method recovery, μ(R) that was estimated within 0.0064-0.0995pmolμl(-1). The uncertainties from other sources, μ(Std) were 0.0325, 0.0428 and 0.0413pmolμl(-1) that were contributed by hydroxyproline, other amino acids and cystine, respectively. Hydroxyproline and phenylalanine represented the lowest and highest values of expanded uncertainty, U(y) that were determined at 0.0949 and 0.2473pmolμl(-1), respectively. The data were accurately defined and fulfill the technical requirements of ISO 17025:2005.
    MeSH terms: Amino Acids/chemistry*; Aminoquinolines/chemistry*; Carbamates/chemistry*; Gelatin/chemistry*; Hydroxyproline/chemistry; Indicators and Reagents/chemistry; Reference Standards; Reproducibility of Results; Uncertainty; Chromatography, Reverse-Phase/methods
  7. Taha M, Ismail NH, Imran S, Rahim F, Wadood A, Khan H, et al.
    Bioorg Chem, 2016 10;68:56-63.
    PMID: 27454618 DOI: 10.1016/j.bioorg.2016.07.008
    Hybrid bisindole-thiosemicarbazides analogs (1-18) were synthesized and screened for β-glucuronidase activity. All compounds showed varied degree of β-glucuronidase inhibitory potential when compared with standard d-saccharic acid 1,4-lactone (IC50=48.4±1.25μM). Compounds 4, 7, 9, 6, 5, 12, 17 and 18 showed exceptional β-glucuronidase inhibition with IC50 values ranging from 0.1 to 5.7μM. Compounds 1, 3, 8, 16, 13, 2 and 14 also showed better activities than standard with IC50 values ranging from 7.12 to 15.0μM. The remaining compounds 10, 11, and 15 showed good inhibitory potential with IC50 values 33.2±0.75, 21.4±0.30 and 28.12±0.25μM respectively. Molecular docking studies were carried out to confirm the binding interaction of the compounds.
    MeSH terms: Dose-Response Relationship, Drug; Glucuronidase/antagonists & inhibitors*; Glucuronidase/metabolism; Glycoproteins/chemical synthesis; Glycoproteins/pharmacology*; Glycoproteins/chemistry; Humans; Indoles/pharmacology*; Indoles/chemistry; Semicarbazides/pharmacology*; Semicarbazides/chemistry; Structure-Activity Relationship; Molecular Structure; Molecular Docking Simulation*
  8. Lai LC, Lopez J, Kallner A
    PMID: 27454667
  9. Abas A, Gan ZL, Ishak MH, Abdullah MZ, Khor SF
    PLoS One, 2016;11(7):e0159357.
    PMID: 27454872 DOI: 10.1371/journal.pone.0159357
    This paper studies the three dimensional (3D) simulation of fluid flows through the ball grid array (BGA) to replicate the real underfill encapsulation process. The effect of different solder bump arrangements of BGA on the flow front, pressure and velocity of the fluid is investigated. The flow front, pressure and velocity for different time intervals are determined and analyzed for potential problems relating to solder bump damage. The simulation results from Lattice Boltzmann Method (LBM) code will be validated with experimental findings as well as the conventional Finite Volume Method (FVM) code to ensure highly accurate simulation setup. Based on the findings, good agreement can be seen between LBM and FVM simulations as well as the experimental observations. It was shown that only LBM is capable of capturing the micro-voids formation. This study also shows an increasing trend in fluid filling time for BGA with perimeter, middle empty and full orientations. The perimeter orientation has a higher pressure fluid at the middle region of BGA surface compared to middle empty and full orientation. This research would shed new light for a highly accurate simulation of encapsulation process using LBM and help to further increase the reliability of the package produced.
    MeSH terms: Algorithms; Computer Simulation; Models, Theoretical*
  10. Azbel L, Polonsky M, Wegman M, Shumskaya N, Kurmanalieva A, Asanov A, et al.
    Int J Drug Policy, 2016 Nov;37:9-20.
    PMID: 27455177 DOI: 10.1016/j.drugpo.2016.06.007
    BACKGROUND: Central Asia is afflicted with increasing HIV incidence, low antiretroviral therapy (ART) coverage and increasing AIDS mortality, driven primarily by people who inject drugs (PWID). Reliable data about HIV, other infectious diseases, and substance use disorders in prisoners in this region is lacking and could provide important insights into how to improve HIV prevention and treatment efforts in the region.

    METHODS: A randomly sampled, nationwide biobehavioural health survey was conducted in 8 prisons in Kyrgyzstan among all soon-to-be-released prisoners; women were oversampled. Consented participants underwent computer-assisted, standardized behavioural health assessment surveys and testing for HIV, HCV, HBV, and syphilis. Prevalence and means were computed, and generalized linear modelling was conducted, with all analyses using weights to account for disproportionate sampling by strata.

    RESULTS: Among 381 prisoners who underwent consent procedures, 368 (96.6%) were enrolled in the study. Women were significantly older than men (40.6 vs. 36.5; p=0.004). Weighted prevalence (%), with confidence interval (CI), for each infection was high: HCV (49.7%; CI: 44.8-54.6%), syphilis (19.2%; CI: 15.1-23.5%), HIV (10.3%; CI: 6.9-13.8%), and HBV (6.2%; CI: 3.6-8.9%). Among the 31 people with HIV, 46.5% were aware of being HIV-infected. Men, compared to women, were significantly more likely to have injected drugs (38.3% vs.16.0%; p=0.001). Pre-incarceration and within-prison drug injection, primarily of opioids, was 35.4% and 30.8%, respectively. Independent correlates of HIV infection included lifetime drug injection (adjusted odds ratio [AOR]=38.75; p=0.001), mean number of years injecting (AOR=0.93; p=0.018), mean number of days experiencing drug problems (AOR=1.09; p=0.025), increasing duration of imprisonment (AOR=1.08; p=0.02 for each year) and having syphilis (AOR=3.51; p=0.003), while being female (AOR=3.06; p=0.004) and being a recidivist offender (AOR=2.67; p=0.008) were independently correlated with syphilis infection.

    CONCLUSION: Drug injection, syphilis co-infection, and exposure to increased risk during incarceration are likely to be important contributors to HIV transmission among prisoners in Kyrgyzstan. Compared to the community, HIV is concentrated 34-fold higher in prisoners. A high proportion of undiagnosed syphilis and HIV infections presents a significant gap in the HIV care continuum. Findings highlight the critical importance of evidence-based responses within prison, including enhanced testing for HIV and sexually transmitted infections, to stem the evolving HIV epidemic in the region.

    MeSH terms: Adult; Aged; Cross-Sectional Studies; Female; Hepatitis C/diagnosis; Hepatitis C/epidemiology*; Hepatitis C/therapy; Humans; Kyrgyzstan/epidemiology; Male; Middle Aged; Opioid-Related Disorders/epidemiology; Prisoners/statistics & numerical data*; Risk Factors; Syphilis/diagnosis; Syphilis/epidemiology*; Syphilis/therapy; HIV Infections/diagnosis; HIV Infections/epidemiology*; HIV Infections/therapy; Substance Abuse, Intravenous/epidemiology; Prevalence; Multivariate Analysis; Linear Models; Odds Ratio; Young Adult; Epidemics*; Coinfection*
  11. Sukumaran SD, Chee CF, Viswanathan G, Buckle MJ, Othman R, Abd Rahman N, et al.
    Molecules, 2016 Jul 22;21(7).
    PMID: 27455222 DOI: 10.3390/molecules21070955
    A series of 2'-hydroxy- and 2'-hydroxy-4',6'-dimethoxychalcones was synthesised and evaluated as inhibitors of human acetylcholinesterase (AChE). The majority of the compounds were found to show some activity, with the most active compounds having IC50 values of 40-85 µM. Higher activities were generally observed for compounds with methoxy substituents in the A ring and halogen substituents in the B ring. Kinetic studies on the most active compounds showed that they act as mixed-type inhibitors, in agreement with the results of molecular modelling studies, which suggested that they interact with residues in the peripheral anionic site and the gorge region of AChE.
    MeSH terms: Acetylcholinesterase/metabolism; Binding Sites; Cholinesterase Inhibitors/chemical synthesis; Cholinesterase Inhibitors/pharmacology*; Cholinesterase Inhibitors/chemistry*; Enzyme Activation/drug effects; Humans; Hydrogen Bonding; Hydrolysis; Models, Molecular*; Molecular Conformation; Protein Binding; Structure-Activity Relationship; Molecular Structure; Chalcones/chemical synthesis; Chalcones/pharmacology*; Chalcones/chemistry*; Molecular Dynamics Simulation; Chemistry Techniques, Synthetic; Molecular Docking Simulation
  12. Tripathy A, Pramanik S, Manna A, Bhuyan S, Azrin Shah NF, Radzi Z, et al.
    Sensors (Basel), 2016 Jul 21;16(7).
    PMID: 27455263 DOI: 10.3390/s16071135
    Despite the many attractive potential uses of ceramic materials as humidity sensors, some unavoidable drawbacks, including toxicity, poor biocompatibility, long response and recovery times, low sensitivity and high hysteresis have stymied the use of these materials in advanced applications. Therefore, in present investigation, we developed a capacitive humidity sensor using lead-free Ca,Mg,Fe,Ti-Oxide (CMFTO)-based electro-ceramics with perovskite structures synthesized by solid-state step-sintering. This technique helps maintain the submicron size porous morphology of the developed lead-free CMFTO electro-ceramics while providing enhanced water physisorption behaviour. In comparison with conventional capacitive humidity sensors, the presented CMFTO-based humidity sensor shows a high sensitivity of up to 3000% compared to other materials, even at lower signal frequency. The best also shows a rapid response (14.5 s) and recovery (34.27 s), and very low hysteresis (3.2%) in a 33%-95% relative humidity range which are much lower values than those of existing conventional sensors. Therefore, CMFTO nano-electro-ceramics appear to be very promising materials for fabricating high-performance capacitive humidity sensors.
    MeSH terms: Ceramics; Humidity; Oxides; Titanium; Water; Calcium Compounds
  13. Mottalib A, Mohd-Yusof BN, Shehabeldin M, Pober DM, Mitri J, Hamdy O
    Nutrients, 2016 Jul 22;8(7).
    PMID: 27455318 DOI: 10.3390/nu8070443
    Diabetes-specific nutritional formulas (DSNFs) are frequently used as part of medical nutrition therapy for patients with diabetes. This study aims to evaluate postprandial (PP) effects of 2 DSNFs; Glucerna (GL) and Ultra Glucose Control (UGC) versus oatmeal (OM) on glucose, insulin, glucagon-like peptide-1 (GLP-1), free fatty acids (FFA) and triglycerides (TG). After an overnight fast, 22 overweight/obese patients with type 2 diabetes were given 200 kcal of each of the three meals on three separate days in random order. Blood samples were collected at baseline and at 30, 60, 90, 120, 180 and 240 min. Glucose area under the curve (AUC0-240) after GL and UGC was lower than OM (p < 0.001 for both). Insulin positive AUC0-120 after UGC was higher than after OM (p = 0.02). GLP-1 AUC0-120 and AUC0-240 after GL and UGC was higher than after OM (p < 0.001 for both). FFA and TG levels were not different between meals. Intake of DSNFs improves PP glucose for 4 h in comparison to oatmeal of similar caloric level. This is achieved by either direct stimulation of insulin secretion or indirectly by stimulating GLP-1 secretion. The difference between their effects is probably related to their unique blends of amino acids, carbohydrates and fat.
    MeSH terms: Aged; Diabetes Mellitus, Type 2/blood; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/diet therapy*; Diet, Diabetic/methods; Diet, Reducing/methods; Dietary Carbohydrates/therapeutic use; Dietary Fats, Unsaturated/therapeutic use; Female; Food, Formulated*; Hemoglobin A, Glycosylated/analysis; Humans; Hyperglycemia/prevention & control*; Hyperinsulinism/prevention & control*; Hyperlipidemias/prevention & control*; Male; Middle Aged; Seeds/chemistry; Body Mass Index; Avena/chemistry; Cross-Over Studies; Glycemic Index; Overweight/complications; Overweight/diet therapy*; Glucagon-Like Peptide 1/blood; Meals
  14. Nyein N, Tan WK, Kawamura G, Matsuda A, Lockman Z
    Nanotechnology, 2016 Sep 02;27(35):355605.
    PMID: 27456036 DOI: 10.1088/0957-4484/27/35/355605
    Self-organized, 23 μm-thick anodic TiO2 nanotube (TNT) arrays were formed in sodium hydroxide/fluoride/ethylene glycol (EG) electrolyte at 60 V for 60 min. The presence of sodium hydroxide (NaOH) in the fluoride/EG electrolyte accelerates the formation of the TiO2 nanotube arrays. The anodic film was then decorated with silver nanoparticles (Ag NPs) by the photodeposition process and used as a photoanode in a rear-side-illuminated dye-sensitized solar cell. The Ag NPs decorated TNT arrays, with the former having diameters of 10-30 nm formed from 0.2 M of Ag-precursor solution and exhibiting the highest photoconversion efficiency (η) of 3.7% and a short-circuit current density of 12.2 mA cm(-2) compared to η = 3% and short-circuit current density of 9.1 mA cm(-2) for a sample without Ag NPs. The increase in η is thought to be due to the surface plasmon resonance and excess electrons from the nanoparticles.
  15. C SK, M S, K R
    Int J Biol Macromol, 2016 Nov;92:682-693.
    PMID: 27456125 DOI: 10.1016/j.ijbiomac.2016.07.062
    Response Surface Methodology (RSM) was used to optimize the parameters for microwave-assisted extraction of polysaccharides from Cyphomandra betacea. The results showed a good fit with a second-order polynomial equation that was statistically acceptable at P<0.05. Optimal conditions for the extraction of polysaccharides were: extraction time, 2h; microwave power, 400W; extraction temperature, 60°C; and ratio of raw material to water 1:40 (g/mL). Under the optimized conditions, the yield of polysaccharides was found to be relatively high (about 36.52%). The in vitro biological activities of antioxidant and antitumor were evaluated. The IC50 value of polysaccharides was found to be 3mg/mL. The percentage of Cell viability was determined by MTT assay. Our results showed that polysaccharides inhibited proliferation of MCF-7 (Breast carcinoma), A549 (Human lung carcinoma) and HepG2 (Liver carcinoma) with an IC50 of 0.23mg/mL, 0.17mg/mL and 0.62mg/mL respectively after 48h incubation. Polysaccharides were shown to promote apoptosis as seen in the nuclear morphological examination study using acridine orange (AO) and ethidium bromide (EB) staining. This is the first report on the effects of polysaccharides extracted from Cyphomandra betacea which exhibited stronger antioxidant and antitumor activities.
    MeSH terms: Analysis of Variance; Antineoplastic Agents/pharmacology; Antioxidants/pharmacology; Chromatography, Liquid; Humans; Microwaves*; Models, Theoretical; Polysaccharides/isolation & purification*; Polysaccharides/pharmacology*; Polysaccharides/chemistry; Temperature; Time Factors; Water/chemistry; Reproducibility of Results; Apoptosis/drug effects; Spectrometry, Mass, Electrospray Ionization; Solanum/chemistry*; Cell Line, Tumor; Cell Shape/drug effects; Tandem Mass Spectrometry
  16. Baharuddin H, Taib T, Zain MM, Ch'ng S
    Int J Rheum Dis, 2016 Oct;19(10):1035-1038.
    PMID: 27456320 DOI: 10.1111/1756-185X.12916
    Leprosy is a chronic granulomatous infection caused by Mycobacterium leprae with predominant involvement of skin and nerves. We present a 70-year-old man with leprosy whose initial presentation resembled rheumatologic disease, due to leprae reaction. He presented with an 8-week history of worsening neuropathic pain in the right forearm, associated with necrotic skin lesions on his fingers that had ulcerated. Physical examination revealed two tender necrotic ulcers at the tip of the right middle finger and the dorsal aspect of the left middle finger. The patient had right wrist tenosynovitis and right elbow bursitis. Apart from raised inflammatory markers, the investigations for infection, connective tissue disease, vasculitis, thromboembolic disease and malignancy were negative. During the fourth week of hospitalization, we noticed a 2-cm hypoesthetic indurated plaque on the right inner arm. Further examination revealed thickened bilateral ulnar, radial and popliteal nerves. A slit skin smear was negative. Two skin biopsies and a biopsy of the olecranon bursa revealed granulomatous inflammation. He was diagnosed with paucibacillary leprosy with neuritis. He responded well to multidrug therapy and prednisolone; his symptoms resolved over a few weeks. This case illustrates the challenges in diagnosing a case of leprosy with atypical presentation in a non-endemic country.
    MeSH terms: Aged; Biopsy; Diagnosis, Differential; Humans; Leprostatic Agents/therapeutic use; Malaysia; Male; Mycobacterium leprae/isolation & purification*; Predictive Value of Tests; Rheumatic Diseases/diagnosis*; Leprosy, Lepromatous/diagnosis*; Leprosy, Lepromatous/diet therapy; Leprosy, Lepromatous/microbiology; Leprosy, Tuberculoid/diagnosis*; Leprosy, Tuberculoid/drug therapy; Leprosy, Tuberculoid/microbiology; Treatment Outcome
  17. Bhoo-Pathy N, Balakrishnan N, See MH, Taib NA, Yip CH
    World J Surg, 2016 12;40(12):2913-2921.
    PMID: 27456497 DOI: 10.1007/s00268-016-3658-z
    BACKGROUND: Factors associated with surgery, adjuvant radiotherapy, and chemotherapy and whether there were missed opportunities for treatment in elderly patients were determined in an Asian setting.

    METHODS: All 5616 patients, diagnosed with breast cancer in University Malaya Medical Centre from 1999 to 2013 were included. In 945 elderly patients (aged 65 years and above), multivariable logistic regression was performed to identify factors associated with treatment, following adjustment for age, ethnicity, tumor, and other treatment characteristics. The impact of lack of treatment on survival of the elderly was assessed while accounting for comorbidities.

    RESULTS: One in five elderly patients had comorbidities. Compared to younger patients, the elderly had more favorable tumor characteristics, and received less loco-regional treatment and chemotherapy. Within stage I-IIIa elderly breast cancer patients, 10 % did not receive any surgery. These patients were older, more likely to be Malays, have comorbidities, and bigger tumors. In elderlies with indications for adjuvant radiotherapy, no irradiation (30 %) was associated with increasing age, comorbidity, and the absence of systemic therapy. Hormone therapy was optimal, but only 35 % of elderly women with ER negative tumors received chemotherapy. Compared to elderly women who received adequate treatment, those not receiving surgery (adjusted hazard ratio: 2.30, 95 %CI: 1.10-4.79), or radiotherapy (adjusted hazard ratio: 1.56, 95 %CI: 1.10-2.19), were associated with higher mortality. Less than 25 % of the survival discrepancy between elderly women receiving loco-regional treatment and no treatment were attributed to excess comorbidities in untreated patients.

    CONCLUSION: While the presence of comorbidities significantly influenced loco-regional treatment decisions in the elderly, it was only able to explain the lower survival rates in untreated patients up to a certain extent, suggesting missed opportunities for treatment.

    MeSH terms: Age Factors; Aged; Breast Neoplasms/ethnology; Breast Neoplasms/pathology*; Breast Neoplasms/therapy*; Female; Humans; Malaysia; Middle Aged; Neoplasm Staging; Mastectomy, Segmental; Comorbidity*; Survival Rate; Logistic Models; Proportional Hazards Models; Chemotherapy, Adjuvant; Radiotherapy, Adjuvant; Antineoplastic Agents, Hormonal/therapeutic use*; Tumor Burden
  18. Ramli NS, Ismail P, Rahmat A
    BMC Complement Altern Med, 2016 Jul 26;16:243.
    PMID: 27456968 DOI: 10.1186/s12906-016-1200-3
    Red pitaya (Hylocereus polyrhizus) or known as buah naga merah in Malay belongs to the cactus family, Cactaceae. Red pitaya has been shown to give protection against liver damage and may reduce the stiffness of the heart. Besides, the beneficial effects of red pitaya against obesity have been reported; however, the mechanism of this protection is not clear. Therefore, in the present study, we have investigated the red pitaya-targeted genes in obesity using high-carbohydrate, high-fat diet-induced metabolic syndrome rat model.
    MeSH terms: Fruit and Vegetable Juices; Animals; Gene Expression Regulation/drug effects; Homeostasis/drug effects*; Obesity/genetics*; Obesity/metabolism; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Metabolic Syndrome X/genetics*; Metabolic Syndrome X/metabolism; Cactaceae/chemistry*; Abdominal Fat/drug effects; Rats; Diet, High-Fat*
  19. Oroji A, Omar M, Yarahmadian S
    J Theor Biol, 2016 10 21;407:128-137.
    PMID: 27457094 DOI: 10.1016/j.jtbi.2016.07.035
    In this paper, a new mathematical model is proposed for studying the population dynamics of breast cancer cells treated by radiotherapy by using a system of stochastic differential equations. The novelty of the model is essentially in capturing the concept of the cell cycle in the modeling to be able to evaluate the tumor lifespan. According to the cell cycle, each cell belongs to one of three subpopulations G, S, or M, representing gap, synthesis and mitosis subpopulations. Cells in the M subpopulation are highly radio-sensitive, whereas cells in the S subpopulation are highly radio-resistant. Therefore, in the process of radiotherapy, cell death rates of different subpopulations are not equal. In addition, since flow cytometry is unable to detect apoptotic cells accurately, the small changes in cell death rate in each subpopulation during treatment are considered. Subsequently, the proposed model is calibrated using experimental data from previous experiments involving the MCF-7 breast cancer cell line. Consequently, the proposed model is able to predict tumor lifespan based on the number of initial carcinoma cells. The results show the effectiveness of the radiation under the condition of stability, which describes the decreasing trend of the tumor cells population.
    MeSH terms: Algorithms; Breast Neoplasms/pathology*; Breast Neoplasms/radiotherapy*; Calibration; Cell Cycle; Computer Simulation; Female; Humans; Models, Biological*; Monte Carlo Method; Stochastic Processes; Confidence Intervals; MCF-7 Cells
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