Browse publications by year: 2017

  1. Periasamy U, Mohd Sidik S, Rampal L, Fadhilah SI, Akhtari-Zavare M, Mahmud R
    Health Qual Life Outcomes, 2017 May 15;15(1):104.
    PMID: 28506305 DOI: 10.1186/s12955-017-0680-2
    BACKGROUND: Cancer is now becoming a leading cause of death. Chemotherapy is an important treatment for cancer patients. These patients also need consultation during their treatment to improve quality of life and decrease psychological disorders. The objectives of the study were to develop, implement and evaluate the effectiveness of a chemotherapy counseling module by pharmacists among oncology patients on their quality of life and psychological outcomes in Malaysia.

    METHOD: A single-blind randomized controlled trial was carried out among 162 oncology patients undergoing chemotherapy from July 2013 to February 2014 in a government hospital with oncology facilities in Malaysia. Participants were randomized to either the intervention group or the control group. Chemotherapy counseling using the module on 'Managing Patients on Chemotherapy' by Pharmacists was delivered to the intervention group. The outcome measures were assessed at baseline, first follow-up and second follow-up and third follow-up post-intervention. Chi-square, independent samples t-test and two-way repeated measures ANOVA were conducted in the course of the data analyses.

    RESULTS: In assessing the impact of the chemotherapy counseling module, the study revealed that the module along with repetitive counseling showed significant improvement of quality of life in the intervention group as compared to the control group with a large effect size in physical health (p = 0.001, partial Ƞ2 = 0.66), psychological (p = 0.001, partial Ƞ2 = 0.65), social relationships (p = 0.001, partial Ƞ2 = 0.30), and environment (p = 0.001, partial Ƞ2 = 0.67) and decrease in the anxiety (p = 0.000; partial Ƞ2 = 0.23), depression (p = 0.000; partial Ƞ2 = 0.40).

    CONCLUSION: The module on 'Managing Patients on Chemotherapy' along with repetitive counseling by pharmacists has been shown to be effective in improving quality of life and decreasing anxiety and depression among oncology patients undergoing chemotherapy.

    TRIAL REGISTRATION NUMBER: National Medical Research Register (NMRR) of Malaysia and given a registration number NMRR-12-1057-12,363 on 21 December 2012.

    MeSH terms: Aged; Analysis of Variance; Anxiety/psychology; Anxiety/therapy; Counseling/methods*; Depression/psychology; Depression/therapy; Female; Humans; Malaysia; Male; Middle Aged; Neoplasms/drug therapy; Neoplasms/psychology*; Pharmacists*; Quality of Life*; Surveys and Questionnaires; Single-Blind Method; Outcome Assessment (Health Care)
  2. Wong WK, Foo PC, Olivos-Garcia A, Noordin R, Mohamed Z, Othman N, et al.
    Acta Trop, 2017 Aug;172:208-212.
    PMID: 28506795 DOI: 10.1016/j.actatropica.2017.05.017
    Crude soluble antigen (CSA) produced from Entamoeba histolytica trophozoite is conventionally used for serodiagnosis of invasive amoebiasis. However, high background seropositivities by CSA-assay in endemic areas complicate the interpretation of positive result in clinical settings. Instead, incorporating a second assay which indicates active or recent infection into the routine amoebic serology could possibly complement the limitations of CSA-assay. Hence, the present study aimed to evaluate the diagnostic efficacies of indirect ELISAs using CSA and excretory-secretory antigen (ESA) for serodiagnosis of amoebic liver abscess (ALA). Reference standard for diagnosis of ALA at Hospital Universiti Sains Malaysia is based on clinical presentation, radiological imaging and positive indirect haemagglutination assay (titer ≥256). Five groups of human serum samples collected from the hospital included Group I - ALA diagnosed by the reference standard and pus aspirate analysis using real-time PCR (n=10), Group II - ALA diagnosed by the reference standard only (n=41), Group III - healthy control (n=45), Group IV - other diseases control (n=51) and Group V - other infectious diseases control (n=31). For serodiagnosis of ALA serum samples (Group I and II), CSA-ELISA showed sensitivities of 100% for both groups, while ESA-ELISA showed sensitivities of 100% and 88%, respectively. For serodiagnosis of non-ALA serum samples (Group III, IV and V), CSA-ELISA showed specificities of 91%, 75% and 100%, respectively; while ESA-ELISA showed specificities of 96%, 98% and 100%, respectively. Indirect ELISAs using CSA and ESA have shown distinct strength for serodiagnosis of ALA, in terms of sensitivity and specificity, respectively. In conclusion, parallel analysis by both assays improved the overall efficacies of amoebic serology as compared to either single assay.
    MeSH terms: Animals; Antibodies, Protozoan/blood*; Antigens, Protozoan/immunology*; Enzyme-Linked Immunosorbent Assay/methods*; Hemagglutination Tests; Humans; Liver Abscess, Amebic/blood; Liver Abscess, Amebic/diagnosis*; Liver Abscess, Amebic/epidemiology; Liver Abscess, Amebic/parasitology; Malaysia/epidemiology; Sensitivity and Specificity; Serologic Tests/methods; Real-Time Polymerase Chain Reaction
  3. Nijman V, Shepherd CR
    J Ethnopharmacol, 2017 Jul 12;206:101-106.
    PMID: 28506903 DOI: 10.1016/j.jep.2017.05.010
    ETHNOPHARMACOLOGICAL RELEVANCE: Wild animals are widely used in traditional Asian medicine but information from Myanmar is lacking. We show that a wide range of animals are used at a pilgrimage site, mostly for their rendered fats and oils to be used in mixed concoctions. The majority of species were sold to be used to treat aching joints, muscle ache and skin diseases.

    AIM OF THE STUDY: To assess wildlife for sale for medicinal purposes, and document their medicinal use at Kyaiktiyo, a pilgrimage site at a 1100m tall mountain, with many of the pilgrims climbing to the top. In addition we address legal issues relating to the production and sale of traditional medicine that contain legally protected animals.

    MATERIAL AND METHODS: Four visits were made to Kyaiktiyo, Myanmar, between 2000 and 2017 to quantify animal parts on display and through discussions with vendors to obtain information on medicinal use of these parts.

    RESULTS: Twenty-three species, mostly mammals, were recorded to be used for traditional medicine. The most common were Chinese serow Capricornis milneedwardsii, Asian elephant Elephas maximus, and Asiatic black bear Ursus thibetanus. Over 600 bodies or body parts were present. Combined, these parts purportedly provided cures or relief for at least 15 ailments or diseases. The most commonly mentioned treatment was that of using rendered animal fats/oils externally to relieve/cure aching joints or muscles. This treatment allegedly provides instant relief to pilgrims after an arduous climb up the mountain. Purported cures for various skin diseases was the next common use for the animal species on offer. Ten of the species observed for sale at Kyaiktiyo are listed as globally threatened, and 15 are protected and cannot be legally traded. Ambiguities in Myanmar's legislation mean that protected animals or their body parts cannot be traded, however traditional medicines can be made out of them provided rules relating to the manufacturing of traditional medicines are adhered to.

    CONCLUSION: This study indicated that animals and their parts continue to be openly offered for sale at Kyaiktiyo to treat various illnesses. Despite these products potential medical, traditional or cultural importance, solutions have to been found on how to ensure that, in line with Myanmar's laws, use of traditional local medicine does not impede the conservation of imperilled species.

    MeSH terms: Animals; Animals, Wild*; Myanmar; Medicine, East Asian Traditional*
  4. Cartland SP, Harith HH, Genner SW, Dang L, Cogger VC, Vellozzi M, et al.
    Sci Rep, 2017 05 15;7(1):1898.
    PMID: 28507343 DOI: 10.1038/s41598-017-01721-4
    Non-alcoholic fatty liver disease (NAFLD) incorporates steatosis, non-alcoholic steato-hepatitis (NASH) and liver cirrhosis, associating with diabetes and cardiovascular disease (CVD). TNF-related apoptosis-inducing ligand (TRAIL) is protective of CVD. We aimed to determine whether TRAIL protects against insulin resistance, NAFLD and vascular injury. Twelve-week high fat diet (HFD)-fed Trail -/- mice had increased plasma cholesterol, insulin and glucose compared to wildtype. Insulin tolerance was impaired with TRAIL-deletion, with reduced p-Akt, GLUT4 expression and glucose uptake in skeletal muscle. Hepatic triglyceride content, inflammation and fibrosis were increased with TRAIL-deletion, with elevated expression of genes regulating lipogenesis and gluconeogenesis. Moreover, Trail -/- mice exhibited reduced aortic vasorelaxation, impaired insulin signaling, and >20-fold increased mRNA expression for IL-1β, IL-6, and TNF-α. In vitro, palmitate treatment of hepatocytes increased lipid accumulation, inflammation and fibrosis, with TRAIL mRNA significantly reduced. TRAIL administration inhibited palmitate-induced hepatocyte lipid uptake. Finally, patients with NASH had significantly reduced plasma TRAIL compared to control, simple steatosis or obese individuals. These findings suggest that TRAIL protects against insulin resistance, NAFLD and vascular inflammation. Increasing TRAIL levels may be an attractive therapeutic strategy, to reduce features of diabetes, as well as liver and vascular injury, so commonly observed in individuals with NAFLD.
    MeSH terms: Adult; Aged; Animals; Body Weights and Measures; Diabetes Mellitus; Disease Models, Animal; Female; Glucose/metabolism; Humans; Insulin/metabolism; Insulin Resistance*; Liver Function Tests; Male; Middle Aged; Vasculitis/complications*; Vasculitis/metabolism; Vasculitis/pathology; Biomarkers; Gene Deletion*; Mice, Knockout; Lipid Metabolism; Mice; TNF-Related Apoptosis-Inducing Ligand/blood; TNF-Related Apoptosis-Inducing Ligand/deficiency*; TNF-Related Apoptosis-Inducing Ligand/metabolism; Diet, High-Fat; Non-alcoholic Fatty Liver Disease/diagnosis; Non-alcoholic Fatty Liver Disease/etiology*; Non-alcoholic Fatty Liver Disease/metabolism*
  5. Alhammami M, Ooi CP, Tan WH
    Data Brief, 2017 Jun;12:480-484.
    PMID: 28508027 DOI: 10.1016/j.dib.2017.04.026
    We present in this paper a novel dataset (MMU VAAC) for violent actions against children recognition. This original dataset has been recorded using Microsoft Kinect with the usage of a child mannequin. MMU VAAC dataset contains skeleton joints, depth, and RGB modalities.
    MeSH terms: Child; Humans; Manikins; Musculoskeletal System; Skeleton
  6. Alt F, Chong PW, Teng E, Uebelhack R
    Phytother Res, 2017 Jul;31(7):1056-1062.
    PMID: 28508427 DOI: 10.1002/ptr.5826
    Irritable bowel syndrome (IBS) is a functional bowel disorder of unknown aetiology. There is currently no known cure, and pharmacological interventions are usually targeting symptomatic relief, where natural and herbal remedies also play a role. This study aimed to evaluate the benefit and tolerability of IQP-CL-101 in symptomatic IBS relief. A double-blinded, randomised, placebo-controlled trial was conducted over 8 weeks. A total of 99 subjects fulfilling ROME-III criteria for IBS were randomised into two groups, given either two IQP-CL-101 softgels or matching placebo twice daily before main meals. The primary endpoint was the difference in change of IBS Symptom Severity Score (IBS-SSS) after an 8-week intake of IQP-CL-101 compared to placebo. After 8 weeks, subjects on IQP-CL-101 showed a significant reduction in IBS-SSS (113.0 ± 64.9-point reduction) compared to subjects on placebo (38.7 ± 64.5-point reduction) (p 
    MeSH terms: Adult; Biological Products/therapeutic use*; Double-Blind Method; Female; Gastrointestinal Agents/therapeutic use*; Humans; Male; Phytotherapy; Middle Aged; Quality of Life; Abdominal Pain/drug therapy; Treatment Outcome; Irritable Bowel Syndrome/drug therapy*
  7. Saksit N, Tassaneeyakul W, Nakkam N, Konyoung P, Khunarkornsiri U, Chumworathayi P, et al.
    Pharmacogenet Genomics, 2017 07;27(7):255-263.
    PMID: 28509689 DOI: 10.1097/FPC.0000000000000285
    BACKGROUND: Allopurinol is one of the most common causes of severe cutaneous adverse drug reactions (SCARs) including drug reactions with eosinophilia and systemic symptoms (DRESS), Stevens-Johnson syndrome (SJS), and toxic epidermal necrolysis (TEN). This study identified the risk factors associated with the development of allopurinol-induced SCARs in a Thai population.

    PATIENTS AND METHODS: Eighty-six allopurinol-induced SCARs (i.e. 19 DRESS and 67 SJS/TEN) and 182 allopurinol-tolerant patients were enrolled in the study. The HLA-B*58:01 allele was determined. Clinical and medicinal data were collected.

    RESULTS: Results from multivariate analysis showed that only the HLA-B*58:01 and female sex were identified as risk factors of allopurinol-induced SCARs in this Thai population. Patients who carried the HLA-B*58:01 allele were at a higher risk of allopurinol-induced DRESS [odds ratio (OR)=149.2, 95% confidence interval (CI)=24.0-∞, P<1.00×10]. Similar results were observed in allopurinol-induced SJS/TEN (OR=175.0, 95% CI=44.3-690.9, P=1.69×10). The risk of allopurinol-induced SCARs in women was higher than that in men (OR=4.6, 95% CI=1.4-15.6, P=1.44×10). The overall mortality rate of allopurinol-induced SCARs was 11.39% and a higher mortality rate was observed in elderly women.

    CONCLUSION: Among the risk factors identified, the HLA-B*58:01 allele had the greatest impact on the development of both phenotypes of allopurinol-induced SCARs in this studied Thai population. In case HLA-B*58:01 genotyping cannot be accessed, close monitoring of allopurinol usage, especially in elderly women with impaired renal function, is necessary to reduce the mortality rate of these life-threatening SCARs.

    MeSH terms: Aged; Alleles; Allopurinol/adverse effects*; Female; Humans; Male; Middle Aged; Risk Factors; Skin Diseases/chemically induced*; Skin Diseases/epidemiology*; Thailand/epidemiology; Multivariate Analysis
  8. Fartas FM, Abdullah J, Yusof NA, Sulaiman Y, Saiman MI
    Sensors (Basel), 2017 May 16;17(5).
    PMID: 28509848 DOI: 10.3390/s17051132
    In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.
  9. Toh TH, Lim BC, Bujang MAB, Haniff J, Wong SC, Abdullah MR
    Pediatr Int, 2017 Aug;59(8):861-868.
    PMID: 28510345 DOI: 10.1111/ped.13325
    BACKGROUND: We examined the parental perception and accuracy of the Mandarin translation of the Parents' Evaluation of Developmental Status, a screening questionnaire for parent concerns about children's various developmental skills.

    METHODS: The questionnaire was translated into Mandarin. Upon enrollment, caregivers completed the Mandarin PEDS and answered four questions about its acceptability and usefulness, and its ease of understanding and completion. The Mandarin PEDS was independently evaluated by a pediatrician and a community nurse, and classified as high risk (≥two predictive concerns), medium risk (one predictive concern), low risk (any non-predictive concerns) or no risk (if no concern) for developmental delays. The caregivers repeated Mandarin PEDS at a 2 week interval for test-retest reliability, while the children underwent testing for accuracy using a developmental assessment test.

    RESULTS: The majority (≥85%) of the 73 caregivers perceived the Mandarin PEDS as acceptable and useful, as well as easy to understand and complete. Fifteen (20.5%) and 24 responses (33.9%) were classified as high and moderate risk, respectively. The test-retest and inter-rater reliabilities were excellent, with an intra-class correlation coefficient of 0.812 (95% CI: 0.701-0.881, P 

    MeSH terms: Attitude to Health; Child; Developmental Disabilities/diagnosis*; Child, Preschool; China; Cross-Sectional Studies; Female; Humans; Infant; Male; Parents*; Surveys and Questionnaires*; Sensitivity and Specificity; Translations; Reproducibility of Results; Observer Variation; Logistic Models; Risk Assessment; Early Diagnosis
  10. Chong SE, Mohd Nikman A, Saedah A, Wan Mohd Nazaruddin WH, Kueh YC, Lim JA, et al.
    Br J Anaesth, 2017 05 01;118(5):799-801.
    PMID: 28510752 DOI: 10.1093/bja/aex108
    MeSH terms: Adolescent; Adult; Aged; Anesthesia, Spinal/methods*; Computer Systems; Female; Humans; Male; Middle Aged; Needles; Palpation; Ultrasonography, Interventional/methods*; Young Adult
  11. Watts MP, Spurr LP, Gan HM, Moreau JW
    Appl Microbiol Biotechnol, 2017 Jul;101(14):5889-5901.
    PMID: 28510801 DOI: 10.1007/s00253-017-8313-6
    Thiocyanate (SCN-) forms as a by-product of cyanidation during gold ore processing and can be degraded by a variety of microorganisms utilizing it as an energy, nitrogen, sulphur and/or carbon source. In complex consortia inhabiting bioreactor systems, a range of metabolisms are sustained by SCN- degradation; however, despite the addition or presence of labile carbon sources in most bioreactor designs to date, autotrophic bacteria have been found to dominate key metabolic functions. In this study, we cultured an autotrophic SCN--degrading consortium directly from gold mine tailings. In a batch-mode bioreactor experiment, this consortium degraded 22 mM SCN-, accumulating ammonium (NH4+) and sulphate (SO42-) as the major end products. The consortium consisted of a diverse microbial community comprised of chemolithoautotrophic members, and despite the absence of an added organic carbon substrate, a significant population of heterotrophic bacteria. The role of eukaryotes in bioreactor systems is often poorly understood; however, we found their 18S rRNA genes to be most closely related to sequences from bacterivorous Amoebozoa. Through combined chemical and phylogenetic analyses, we were able to infer roles for key microbial consortium members during SCN- biodegradation. This study provides a basis for understanding the behaviour of a SCN- degrading bioreactor under autotrophic conditions, an anticipated approach to remediating SCN- at contemporary gold mines.
    MeSH terms: Bacteria/genetics; Bacteria/metabolism; Biodegradation, Environmental; Carbon/metabolism; Environmental Microbiology*; Mining; RNA, Ribosomal, 18S; Sulfates/metabolism; Thiocyanates/metabolism*; Bioreactors*; Autotrophic Processes*; Chemoautotrophic Growth; Eukaryota/genetics; Eukaryota/metabolism; Amoebozoa/genetics; Carbon Cycle; Microbial Consortia/physiology*; Ammonium Compounds/metabolism
  12. Gagliano MC, Ismail SB, Stams AJM, Plugge CM, Temmink H, Van Lier JB
    Water Res, 2017 09 15;121:61-71.
    PMID: 28511041 DOI: 10.1016/j.watres.2017.05.016
    For the anaerobic biological treatment of saline wastewater, Anaerobic Digestion (AD) is currently a possibility, even though elevated salt concentrations can be a major obstacle. Anaerobic consortia and especially methanogenic archaea are very sensitive to fluctuations in salinity. When working with Upflow Sludge Blanket Reactor (UASB) technology, in which the microorganisms are aggregated and retained in the system as a granular biofilm, high sodium concentration negatively affects aggregation and consequently process performances. In this research, we analysed the structure of the biofilm and granules formed during the anaerobic treatment of high salinity (at 10 and 20 g/L of sodium) synthetic wastewater at lab scale. The acclimated inoculum was able to accomplish high rates of organics removal at all the salinity levels tested. 16S rRNA gene clonal analysis and Fluorescence In Situ Hybridization (FISH) analyses identified the acetoclastic Methanosaeta harundinacea as the key player involved acetate degradation and microbial attachment/granulation. When additional calcium (1 g/L) was added to overcome the negative effect of sodium on microbial aggregation, during the biofilm formation process microbial attachment and acetate degradation decreased. The same result was observed on granules formation: while calcium had a positive effect on granules strength when added to UASB reactors, Methanosaeta filaments were not present and the degradation of the partially acidified substrate was negatively influenced. This research demonstrated the possibility to get granulation at high salinity, bringing to the forefront the importance of a selection towards Methanosaeta cells growing in filamentous form to obtain strong and healthy granules.
    MeSH terms: Anaerobiosis; RNA, Ribosomal, 16S; Sewage; Waste Disposal, Fluid*; In Situ Hybridization, Fluorescence; Biofilms*; Bioreactors; Salinity*
  13. Refsum E, Mörtberg A, Dahl J, Meinke S, Auvinen MK, Westgren M, et al.
    Transfus Med, 2017 Feb;27(1):43-51.
    PMID: 27862486 DOI: 10.1111/tme.12375
    OBJECTIVES: To investigate the specificities and level of HLA class I antibodies in selected cases referred for suspected foetal and neonatal alloimmune thrombocytopenia (FNAIT).

    BACKGROUND: FNAIT occurs in 1 : 1-2000 live births, whereas maternal immunisation against human leukocyte antigen (HLA) class I is common. Whether HLA class I antibodies alone can cause FNAIT is debatable.

    MATERIAL AND METHODS: A total of 260 patient samples were referred between 2007 and 2012. Referrals with maternal HLA class I antibodies and no other cause for the neonatal thrombocytopenia were included for analysis (cases, n = 23). HPA-1a negative mothers were excluded. Control groups were screened positive mothers of healthy neonates (controls, n = 33) and female blood donors (blood donors, n = 19). LABScreen single antigen HLA class I beads was used for antibody analysis. Clinical records were reviewed for cases.

    RESULTS: All groups had broad antibody reactivity. Cases had more antibodies with high SFI levels compared with the controls (SFI>9999; medians 26, 6 and 0; P 

    MeSH terms: Autoantibodies/blood*; Female; Fetomaternal Transfusion/blood*; Fetomaternal Transfusion/complications; Humans; Infant, Newborn; Male; Pregnancy; Histocompatibility Antigens Class I*; Thrombocytopenia, Neonatal Alloimmune/blood*; Thrombocytopenia, Neonatal Alloimmune/etiology
  14. Seyedan A, Alshawsh MA, Alshagga MA, Mohamed Z
    Planta Med, 2017 May;83(8):684-692.
    PMID: 27992939 DOI: 10.1055/s-0042-121754
    The present study investigated the antiobesity and lipid lowering effects of an ethanolic extract of leaves obtained from Orthosiphon stamineus (200 and 400 mg/kg) and its major compound (rosmarinic acid, 10 mg/kg) in obese mice (C57BL/6) induced by a high-fat diet. Continuous supplementation with O. stamineus extract (200 and 400 mg/kg) for 8 weeks significantly decreased body weight gain (p 
    MeSH terms: Animals; Antioxidants/metabolism; Cinnamates/therapeutic use; Insulin/blood; Lipids/blood; Male; Phytotherapy*; Mice, Inbred C57BL; Mice, Obese; Obesity/blood; Obesity/drug therapy*; Plant Extracts/therapeutic use*; Lipid Peroxidation; Anti-Obesity Agents/therapeutic use*; Orthosiphon/chemistry*; Mice; Adiponectin/blood; Depsides/therapeutic use; Diet, High-Fat
  15. Loh SY, Giribabu N, Gholami K, Salleh N
    Arch Biochem Biophys, 2017 Jan 15;614:41-49.
    PMID: 28024836 DOI: 10.1016/j.abb.2016.12.008
    We hypothesized that higher blood pressure in males than females could be due to testosterone effects on aquaporin (AQP) expression in kidneys.

    METHODS: Orchidectomized adult male Sprague-Dawley (SD) rats received seven days subcutaneous testosterone treatment (125 μg/kg/day or 250 μg/kg/day), with or without flutamide or finasteride. Following completion of treatment, MAP was determined in rats under anaesthesia via carotid artery cannulation. In another cohort of rats, kidneys were removed following sacrifice and AQP-1, 2, 3, 4, 6 and 7 protein and mRNA levels were determined by Western blotting and Real-time PCR respectively. Distribution of AQP subunits' protein in the nephrons were visualized by immunofluorescence.

    RESULTS: Testosterone caused MAP, AQP-1, 2, 4, 6 and 7 protein and mRNA levels in kidneys to increase while AQP-3 protein and mRNA levels in kidneys to decrease (p 

    MeSH terms: Animals; Blood Pressure; Flutamide/chemistry; Gene Expression Regulation; Kidney Tubules/metabolism*; Kidney Tubules, Collecting/metabolism*; Male; Microscopy, Fluorescence; Orchiectomy; RNA, Messenger/metabolism; Testosterone/chemistry*; Cohort Studies; Rats, Sprague-Dawley; Finasteride/chemistry; Aquaporins/genetics*; Aquaporins/metabolism*; Rats
  16. Razavi M, Karimian H, Yeong CH, Fadaeinasab M, Khaing SL, Chung LY, et al.
    Drug Des Devel Ther, 2017;11:1-15.
    PMID: 28031701 DOI: 10.2147/DDDT.S115466
    This study aimed to formulate floating gastroretentive tablets containing metformin hydrochloric acid (HCl), using various grades of hydrogel such as tamarind powders and xanthan to overcome short gastric residence time of the conventional dosage forms. Different concentrations of the hydrogels were tested to determine the formulation that could provide a sustained release of 12 h. Eleven formulations with different ratios of tamarind seed powder/tamarind kernel powder (TKP):xanthan were prepared. The physical parameters were observed, and in vitro drug-release studies of the prepared formulations were carried out. Optimal formulation was assessed for physicochemical properties, thermal stability, and chemical interaction followed by in vivo gamma scintigraphy study. MKP3 formulation with a TKP:xanthan ratio of 3:2 was found to have 99.87% release over 12 h. Furthermore, in vivo gamma scintigraphy study was carried out for the optimized formulation in healthy New Zealand White rabbits, and the pharmacokinetic parameters of developed formulations were obtained. 153Sm2O3 was used to trace the profile of release in the gastrointestinal tract of the rabbits, and the drug release was analyzed. The time (Tmax) at which the maximum concentration of metformin HCl in the blood (Cmax) was observed, and it was extended four times for the gastroretentive formulation in comparison with the formulation without polymers. Cmax and the half-life were found to be within an acceptable range. It is therefore concluded that MKP3 is the optimal formulation for sustained release of metformin HCl over a period of 12 h as a result of its floating properties in the gastric region.
    MeSH terms: Administration, Oral; Animals; Gamma Rays*; Male; Metformin/administration & dosage*; Metformin/analysis*; Metformin/blood; Metformin/pharmacokinetics; Rabbits; Radioisotopes; Radionuclide Imaging*; Samarium; Seeds/chemistry*; Reproducibility of Results; Tamarindus/chemistry*; Gastrointestinal Tract/metabolism*
  17. Makaremi M, Pasbakhsh P, Cavallaro G, Lazzara G, Aw YK, Lee SM, et al.
    ACS Appl Mater Interfaces, 2017 May 24;9(20):17476-17488.
    PMID: 28481104 DOI: 10.1021/acsami.7b04297
    Pectin bionanocomposite films filled with various concentrations of two different types of halloysite nanotubes were prepared and characterized in this study as potential films for food packaging applications. The two types of halloysite nanotubes were long and thin (patch) (200-30 000 nm length) and short and stubby (Matauri Bay) (50-3000 nm length) with different morphological, physical, and dispersibility properties. Both matrix (pectin) and reinforcer (halloysite nanotubes) used in this study are considered as biocompatible, natural, and low-cost materials. Various characterization tests including Fourier transform infrared spectroscopy, field emission scanning electron microscopy, release kinetics, contact angle, and dynamic mechanical analysis were performed to evaluate the performance of the pectin films. Exceptional thermal, tensile, and contact angle properties have been achieved for films reinforced by patch halloysite nanotubes due to the patchy and lengthy nature of these tubes, which form a bird nest structure in the pectin matrix. Matauri Bay halloysite nanotubes were dispersed uniformly and individually in the matrix in low and even high halloysite nanotube concentrations. Furthermore, salicylic acid as a biocidal agent was encapsulated in the halloysite nanotubes lumen to control its release kinetics. On this basis, halloysite nanotubes/salicylic acid hybrids were dispersed into the pectin matrix to develop functional biofilms with antimicrobial properties that can be extended over time. Results revealed that shorter nanotubes (Matauri Bay) had better ability for the encapsulation of salicylic acid into their lumen, while patchy structure and longer tubes of patch halloysite nanotubes made the encapsulation process more difficult, as they might need more time and energy to be fully loaded by salicylic acid. Moreover, antimicrobial activity of the films against four different strains of Gram-positive and Gram-negative bacteria indicated the effective antimicrobial properties of pectin/halloysite functionalized films and their potential to be used for food packaging applications.
    MeSH terms: Aluminum Silicates; Pectins; Food Packaging; Nanotubes*
  18. See-Too WS, Salazar S, Ee R, Convey P, Chan KG, Peix Á
    Syst Appl Microbiol, 2017 Jun;40(4):191-198.
    PMID: 28501448 DOI: 10.1016/j.syapm.2017.03.002
    In this study we analysed three bacterial strains coded L10.10T, A4R1.5 and A4R1.12, isolated in the course of a study of quorum-quenching bacteria occurring in Antarctic soil. The 16S rRNA gene sequence was identical in the three strains and showed 99.7% pairwise similarity with respect to the closest related species Pseudomonas weihenstephanensis WS4993T. Therefore, the three strains were classified within the genus Pseudomonas. Analysis of housekeeping genes (rpoB, rpoD and gyrB) sequences showed similarities of 84-95% with respect to the closest related species of Pseudomonas, confirming its phylogenetic affiliation. The ANI values were less than 86% to the closest related species type strains. The respiratory quinone is Q9. The major fatty acids are C16:0, C16:1 ω7c/ C16:1 ω6c in summed feature 3 and C18:1 ω7c / C18:1 ω6c in summed feature 8. The strains are oxidase- and catalase-positive. Growth occurs at 4-30°C, and at pH 4.0-10. The DNA G+C content is 58.2-58.3mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains L10.10T, A4R1.5 and A4R1.12 into a novel species of Pseudomonas, for which the name P. versuta sp. nov. is proposed. The type strain is L10.10T (LMG 29628T, DSM 101070T).
    MeSH terms: Antarctic Regions; Base Composition/genetics; Base Sequence; DNA, Bacterial/genetics; Fatty Acids/analysis; Phylogeny; DNA-Directed RNA Polymerases/genetics; RNA, Ribosomal, 16S/genetics; Soil; Soil Microbiology*; Bacterial Typing Techniques; Sequence Analysis, DNA; DNA Gyrase/genetics; Quorum Sensing/genetics*
  19. Mani V, Jaafar SM, Azahan NSM, Ramasamy K, Lim SM, Ming LC, et al.
    Life Sci, 2017 Jul 01;180:23-35.
    PMID: 28501482 DOI: 10.1016/j.lfs.2017.05.013
    AIM: The present study is aimed to investigate the ability of ciproxifan, a histamine H3 receptor antagonist to inhibit β-amyloid (Aβ)-induced neurotoxicity in SK-N-SH cells and APP transgenic mouse model.

    MATERIALS AND METHODS: In vitro studies was designed to evaluate the neuroprotective effects of ciproxifan in Aβ25-35 - induced SK-N-SH cells. For the in vivo study, ciproxifan (1 and 3mg/kg, i.p.) was administrated to transgenic mice for 15days and behaviour was assessed using the radial arm maze (RAM). Brain tissues were collected to measure Aβ levels (Aβ1-40 and Aβ1-42), acetylcholine (ACh), acetylcholinesterase (AChE), nitric oxide (NO), lipid peroxidation (LPO), antioxidant activities, cyclooxygenases (COX) and cytokines (IL-1α, IL-1β and IL-6), while plasma was collected to measure TGF-1β.

    RESULTS: The in vitro studies demonstrated neuroprotective effect of ciproxifan by increasing cell viability and inhibiting reactive oxygen species (ROS) in Aβ25-35-induced SK-N-SH cells. Ciproxifan significantly improved the behavioural parameters in RAM. Ciproxifan however, did not alter the Aβ levels in APP transgenic mice. Ciproxifan increased ACh and showed anti-oxidant properties by reducing NO and LPO levels as well as enhancing antioxidant levels. The neuroinflammatory analysis showed that ciproxifan reduced both COX-1 and COX-2 activities, decreased the level of pro-inflammatory cytokines IL-1α, IL-1β and IL-6 and increased the level of anti-inflammatory cytokine TGF-1β.

    CONCLUSION: This present study provides scientific evidence of the use of ciproxifan via antioxidant and cholinergic pathways in the management of AD.

    MeSH terms: Acetylcholine; Acetylcholinesterase; Animals; Antioxidants; Mice, Transgenic; Nitric Oxide; Lipid Peroxidation; Interleukin-6; Cytokines; Reactive Oxygen Species; Neuroprotective Agents; Mice; Cyclooxygenase 2; Histamine H3 Antagonists
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