Browse publications by year: 2017

  1. Zhu F, Storey S, Ashaari MM, Clipson N, Doyle E
    Environ Sci Pollut Res Int, 2017 Feb;24(6):5404-5414.
    PMID: 28025788 DOI: 10.1007/s11356-016-8251-3
    Benzo(a)pyrene degradation was compared in soil that was either composted, incubated at a constant temperature of 22 °C, or incubated under a temperature regime typical of a composting process. After 84 days, significantly more (61%) benzo(a)pyrene was removed from composted soil compared to soils incubated at a constant temperature (29%) or at composting temperatures (46%). Molecular fingerprinting approaches indicated that in composted soils, bacterial community changes were driven by both temperature and organic amendment, while fungal community changes were primarily driven by temperature. Next-generation sequencing data revealed that the bacterial community in composted soil was dominated by Actinobacteria (order Actinomycetales), Firmicutes (class Bacilli), and Proteobacteria (classes Gammaproteobacteria and Alphaproteobacteria), regardless of whether benzo(a)pyrene was present or not. The relative abundance of unclassified Actinomycetales (Actinobacteria) was significantly higher in composted soil when degradation was occurring, indicating a potential role for these organisms in benzo(a)pyrene metabolism. This study provides baseline data for employing straw-based composting strategies for the removal of high molecular weight PAHs from soil and contributes to the knowledge of how microbial communities respond to incubation conditions and pollutant degradation.
    MeSH terms: Benzo(a)pyrene*; Biodegradation, Environmental*; Fungi/metabolism; Polycyclic Hydrocarbons, Aromatic; Soil; Soil Microbiology*; Soil Pollutants*; Temperature; Alphaproteobacteria/metabolism; Actinobacteria/metabolism
  2. Velaithan V, Okuda KS, Ng MF, Samat N, Leong SW, Faudzi SM, et al.
    Invest New Drugs, 2017 04;35(2):166-179.
    PMID: 28058624 DOI: 10.1007/s10637-016-0423-y
    Zebrafish represents a powerful in vivo model for phenotype-based drug discovery to identify clinically relevant small molecules. By utilizing this model, we evaluated natural product derived compounds that could potentially modulate Notch signaling that is important in both zebrafish embryogenesis and pathogenic in human cancers. A total of 234 compounds were screened using zebrafish embryos and 3 were identified to be conferring phenotypic alterations similar to embryos treated with known Notch inhibitors. Subsequent secondary screens using HEK293T cells overexpressing truncated Notch1 (HEK293TΔE) identified 2 compounds, EDD3 and 3H4MB, to be potential Notch antagonists. Both compounds reduced protein expression of NOTCH1, Notch intracellular domain (NICD) and hairy and enhancer of split-1 (HES1) in HEK293TΔE and downregulated Notch target genes. Importantly, EDD3 treatment of human oral cancer cell lines demonstrated reduction of Notch target proteins and genes. EDD3 also inhibited proliferation and induced G0/G1 cell cycle arrest of ORL-150 cells through inducing p27KIP1. Our data demonstrates the utility of the zebrafish phenotypic screen and identifying EDD3 as a promising Notch antagonist for further development as a novel therapeutic agent.
    MeSH terms: Animals; Antineoplastic Agents/pharmacology*; Cell Cycle/drug effects; Cell Survival/drug effects; Curcumin/analogs & derivatives*; Curcumin/pharmacology*; Embryo, Nonmammalian/abnormalities; Embryo, Nonmammalian/drug effects; Humans; Phenotype; Triterpenes/pharmacology*; Zebrafish; Zebrafish Proteins/antagonists & inhibitors; Zebrafish Proteins/metabolism; Cell Line, Tumor; Cell Proliferation/drug effects; Receptors, Notch/antagonists & inhibitors*; Receptors, Notch/metabolism; HEK293 Cells
  3. Liew MH, Ng S, Chew CC, Koo TW, Chee YL, Chee EL, et al.
    Invest New Drugs, 2017 04;35(2):145-157.
    PMID: 28070719 DOI: 10.1007/s10637-016-0415-y
    The sex-divergent pharmacokinetics and interaction of tyrosine kinase inhibitor sunitinib with paracetamol was evaluated in male and female mice. Mice (control groups) were administered 60 mg/kg PO sunitinib alone or with 200 mg/kg PO paracetamol (study groups). Sunitinib concentration in plasma, brain, kidney and liver were determined and non-compartmental pharmacokinetic analysis performed. Female control mice showed 36% higher plasma sunitinib AUC0→∞, 31% and 27% lower liver and kidney AUC0→∞ and 2.2-fold higher AUC0→∞ in brain (all p 
    MeSH terms: Acetaminophen/pharmacology*; Administration, Oral; Animals; Brain/metabolism; Drug Interactions; Female; Indoles/blood; Indoles/pharmacokinetics*; Kidney/metabolism; Liver/metabolism; Male; Mice, Inbred ICR; Pyrroles/blood; Pyrroles/pharmacokinetics*; Sex Characteristics; Analgesics, Non-Narcotic/pharmacology*; Area Under Curve; Angiogenesis Inhibitors/blood; Angiogenesis Inhibitors/pharmacokinetics*; Protein Kinase Inhibitors/blood; Protein Kinase Inhibitors/pharmacokinetics*
  4. Chen LK, Arai H, Chen LY, Chou MY, Djauzi S, Dong B, et al.
    BMC Infect Dis, 2017 03 15;17(1):213.
    PMID: 28298208 DOI: 10.1186/s12879-017-2198-y
    BACKGROUND: Herpes zoster (HZ) is a prevalent viral disease that inflicts substantial morbidity and associated healthcare and socioeconomic burdens. Current treatments are not fully effective, especially among the most vulnerable patients. Although widely recommended, vaccination against HZ is not routine; barriers in Asia-Pacific include long-standing neglect of adult immunisation and sparse local data. To address knowledge gaps, raise awareness, and disseminate best practice, we reviewed recent data and guidelines on HZ from the Asia-Pacific region.

    METHODS: We searched PubMed, Scopus, and World Health Organization databases for articles about HZ published from 1994 to 2014 by authors from Australia, China, Hong Kong, India, Indonesia, Japan, Korea, Malaysia, New Zealand, the Philippines, Singapore, Taiwan, Thailand, and Vietnam. We selected articles about epidemiology, burden, complications, comorbidities, management, prevention, and recommendations/guidelines. Internet searches retrieved additional HZ immunisation guidelines.

    RESULTS: From 4007 retrieved articles, we screened-out 1501 duplicates and excluded 1264 extraneous articles, leaving 1242 unique articles. We found guidelines on adult immunisation from Australia, India, Indonesia, Malaysia, New Zealand, the Philippines, South Korea, and Thailand. HZ epidemiology in Asia-Pacific is similar to elsewhere; incidence rises with age and peaks at around 70 years - lifetime risk is approximately one-third. Average incidence of 3-10/1000 person-years is rising at around 5% per year. The principal risk factors are immunosenescence and immunosuppression. HZ almost always causes pain, and post-herpetic neuralgia is its most common complication. Half or more of hospitalised HZ patients have post-herpetic neuralgia, secondary infections, or inflammatory sequelae that are occasionally fatal. These disease burdens severely diminish patients' quality of life and incur heavy healthcare utilisation.

    CONCLUSIONS: Several countries have abundant data on HZ, but others, especially in South-East Asia, very few. However, Asia-Pacific countries generally lack data on HZ vaccine safety, efficacy and cost-effectiveness. Physicians treating HZ and its complications in Asia-Pacific face familiar challenges but, with a vast aged population, Asia bears a unique and growing burden of disease. Given the strong rationale for prevention, most adult immunisation guidelines include HZ vaccine, yet it remains underused. We urge all stakeholders to give higher priority to adult immunisation in general and HZ in particular.

    MeSH terms: Asia/epidemiology; Cost-Benefit Analysis; Health Surveys; Herpes Zoster/epidemiology*; Herpes Zoster/prevention & control; Humans; Health Knowledge, Attitudes, Practice; Pacific Islands/epidemiology; Vaccination/statistics & numerical data*; Practice Guidelines as Topic; Neuralgia, Postherpetic/epidemiology; Neuralgia, Postherpetic/prevention & control*; Herpes Zoster Vaccine/immunology; Herpes Zoster Vaccine/therapeutic use*; Clinical Audit
  5. Riddell MA, Edwards N, Thompson SR, Bernabe-Ortiz A, Praveen D, Johnson C, et al.
    Global Health, 2017 03 15;13(1):17.
    PMID: 28298233 DOI: 10.1186/s12992-017-0242-8
    BACKGROUND: The imperative to improve global health has prompted transnational research partnerships to investigate common health issues on a larger scale. The Global Alliance for Chronic Diseases (GACD) is an alliance of national research funding agencies. To enhance research funded by GACD members, this study aimed to standardise data collection methods across the 15 GACD hypertension research teams and evaluate the uptake of these standardised measurements. Furthermore we describe concerns and difficulties associated with the data harmonisation process highlighted and debated during annual meetings of the GACD funded investigators. With these concerns and issues in mind, a working group comprising representatives from the 15 studies iteratively identified and proposed a set of common measures for inclusion in each of the teams' data collection plans. One year later all teams were asked which consensus measures had been implemented.

    RESULTS: Important issues were identified during the data harmonisation process relating to data ownership, sharing methodologies and ethical concerns. Measures were assessed across eight domains; demographic; dietary; clinical and anthropometric; medical history; hypertension knowledge; physical activity; behavioural (smoking and alcohol); and biochemical domains. Identifying validated measures relevant across a variety of settings presented some difficulties. The resulting GACD hypertension data dictionary comprises 67 consensus measures. Of the 14 responding teams, only two teams were including more than 50 consensus variables, five teams were including between 25 and 50 consensus variables and four teams were including between 6 and 24 consensus variables, one team did not provide details of the variables collected and two teams did not include any of the consensus variables as the project had already commenced or the measures were not relevant to their study.

    CONCLUSIONS: Deriving consensus measures across diverse research projects and contexts was challenging. The major barrier to their implementation was related to the time taken to develop and present these measures. Inclusion of consensus measures into future funding announcements would facilitate researchers integrating these measures within application protocols. We suggest that adoption of consensus measures developed here, across the field of hypertension, would help advance the science in this area, allowing for more comparable data sets and generalizable inferences.

    MeSH terms: Chronic Disease/therapy*; Cooperative Behavior; Humans; Hypertension; Research Personnel; Global Health*; Consensus*
  6. Fauzi NA, Farid MM, Silva F
    J Food Sci Technol, 2017 Mar;54(3):802-809.
    PMID: 28298695 DOI: 10.1007/s13197-017-2526-7
    This paper investigates the effect of high pressure liquid food compressibility onS. cerevisaeinactivation. Honey with various adjusted sugar with different values of compressibility was selected as a model food.S. cerevisiaecells in different honey concentrations (0-80°Brix), 600 MPa (at ambient temperature) showed an increasing resistance to inactivation with increasing °Brix.D-values ofS. cerevisiaeat 200, 400 and 600 MPa, for 20 min/80°Brix were 136.99 ± 7.97, 29.24 ± 6.44 and 23.47 ± 0.86 min, respectively. TheseD-values resulted the Z
    p
    -value of 526 ± 39 MPa. A significant correlation (p 
    MeSH terms: Honey; Preservation, Biological; Pressure; Temperature
  7. Mazlan-Kepli W, Macisaac RL, Walters M, Bath PMW, Dawson J, VISTA Collaborators
    Br J Clin Pharmacol, 2017 Sep;83(9):2045-2055.
    PMID: 28299795 DOI: 10.1111/bcp.13290
    AIMS: Antiplatelet drugs are often discontinued early after ischaemic stroke, either because of poor compliance, complications or withdrawal of care. It is unclear whether this places patients at increased risk of recurrence. We explored the association between cardiovascular event rate and persistence with prescribed antiplatelet drugs.

    METHODS: We used a matched case-control design using the Virtual International Stroke Trials Archive (VISTA). Cases were patients who had an acute coronary syndrome, recurrent stroke or transient ischaemic attack within 90 days post-stroke and were matched for age ± 10 years and sex with up to four controls. Antiplatelet use was categorized as persistent (used for >3 days and continued up to day 90), early cessation (used antiplatelet <3 days) or stopped/interrupted users (used for >3 days but stopped prior to day 90). These categories were compared in cases and controls using a conditional logistic regression model that adjusted for potential confounders.

    RESULTS: A total of 970 patients were included, of whom 194 were cases and 776 were matched controls. At 90 days, 10 cases (5.2%) and 58 controls (7.5%) stopped/interrupted their antiplatelet. The risk of cardiovascular event was not different in stopped/interrupted users (adjusted odds ratio 0.70, 95% confidence interval 0.33, 1.48; P = 0.352) and early cessations (adjusted odds ratio 1.04, 95% confidence interval 0.62, 1.74; P = 0.876) when compared to persistent users.

    CONCLUSION: We found no increased risk in patients who stopped and interrupted antiplatelets early after stroke but the study was limited by a small sample size and further research is needed.

    MeSH terms: Aged; Ischemic Attack, Transient/complications; Female; Humans; Male; Platelet Aggregation Inhibitors/adverse effects*; Platelet Aggregation Inhibitors/therapeutic use; Recurrence; Case-Control Studies; Stroke/drug therapy; Stroke/epidemiology*; Withholding Treatment/statistics & numerical data*; Acute Coronary Syndrome/complications
  8. Vijayasingham L
    Mult Scler, 2017 09;23(10):1430-1431.
    PMID: 28299951 DOI: 10.1177/1352458517701315
    MeSH terms: Australia; Employment*; Disabled Persons; Humans; Multiple Sclerosis*
  9. Sushma R, Sathe TT, Farias A, Sanyal PK, Kiran S
    Ann Afr Med, 2017;16(1):6-12.
    PMID: 28300045 DOI: 10.4103/aam.aam_43_16
    BACKGROUND: Candida albicans is one of the microorganisms which harbor the oral cavity, especially in elderly. However, the incidence of existence of this increases in patients using removable dental prosthesis. There is therefore a need to test the anticandidal efficacy of these cost-effective, easily available products to be used as routine denture cleansers.

    AIM AND OBJECTIVES: (1) To evaluate antifungal properties of triphala churna on the heat cure denture base material. (2) To evaluate the antifungal effect of chlorhexidine gluconate on the heat cure denture base material. (3) To compare the antifungal effect of triphala churna and chlorhexidine gluconate with a control. (4) To evaluate which among triphala churna and chlorhexidine gluconate has a better antifungal property on the heat cure denture base material.

    MATERIALS AND METHODS: Study population consisted of sixty dentures wearers from those attending the Outpatient Department of Prosthodontics of the School of Dentistry, Krishna Institute of Medical Sciences Deemed University, Karad. Swabs were collected from the dentures before and after the use of triphala and chlorhexidine. The swabs were cultured on Sabouraud dextrose agar and the total Candida counts were determined.

    CONCLUSION: Triphala as an antifungal is shown to have more efficacy than the conventional chlorhexidine mouthwash. Résumé Arrière-plan: Candida albicans est l'un des micro-organismes qui abritent la cavité buccale surtout chez les personnes âgées. Cependant, l'incidence de l'existence de cette augmentation chez les patients utilisant des prothèses dentaires amovibles. Il est donc nécessaire de tester l'efficacité anticancédique de ces produits rentables et faciles à utiliser pour être utilisés comme nettoyants de routine pour prothèses dentaires. Buts et Objectifs: (1) Évaluer les propriétés antifongiques de Triphala churna sur le matériau de base de la prothèse thermo-durcissable. (2) Évaluer l'effet antifongique du gluconate de chlorhexidine sur le matériau de base de la prothèse thermo-durcissable. (3) Comparer l'effet antifongique de Triphala churna et du gluconate de chlorhexidine avec un témoin. (4) Évaluer lequel parmi Triphala churna et le gluconate de chlorhexidine a une meilleure propriété antifongique sur le matériel de base de la prothèse de durcissement à chaud. Matériaux et Méthode: La population de l'étude était constituée de soixante porteurs de prothèses dentaires de ceux qui fréquentaient le Département de Prosthodontie de l'École des Sciences Dentaires de l'Institut Krishna des Sciences Médicales de l'Université de Karad. Des prélèvements ont été effectués sur les prothèses avant et après l'utilisation de Triphala et de chlorhexidine. On a cultivé les écouvillons sur de l'agar Sabouraud dextrose et on a déterminé le nombre total de candida.

    CONCLUSION: Triphala comme un anti fongique est démontré pour avoir plus d'efficacité que le lavage de la bouche classique chlorhexidine.

    MeSH terms: Aged; Aged, 80 and over; Anti-Infective Agents, Local/pharmacology; Anti-Infective Agents, Local/therapeutic use*; Antifungal Agents/pharmacology; Antifungal Agents/therapeutic use*; Candida albicans/drug effects*; Candida albicans/isolation & purification; Candidiasis/drug therapy*; Chlorhexidine/analogs & derivatives*; Chlorhexidine/pharmacology; Chlorhexidine/therapeutic use; Denture Cleansers/pharmacology; Denture Cleansers/therapeutic use*; Denture, Partial, Removable/microbiology; Fruit*; Humans; Middle Aged; Plant Extracts/pharmacology; Plant Extracts/therapeutic use*; Socioeconomic Factors; Colony Count, Microbial; Treatment Outcome; Plant Preparations/therapeutic use; Terminalia/chemistry; Phyllanthus emblica/chemistry
  10. Dzaki N, Ramli KN, Azlan A, Ishak IH, Azzam G
    Sci Rep, 2017 03 16;7:43618.
    PMID: 28300076 DOI: 10.1038/srep43618
    The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with regards to Ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiologically important insect is presently lacking. Here, the usability of seven widely-utilized reference genes i.e. actin (ACT), eukaryotic elongation factor 1 alpha (eEF1α), alpha tubulin (α-tubulin), ribosomal proteins L8, L32 and S17 (RPL8, RPL32 and RPS17), and glyceraldeyde 3-phosphate dehydrogenase (GAPDH) were investigated. Expression patterns of the reference genes were observed in sixteen pre-determined developmental stages and in cell culture. Gene stability was inferred from qPCR data through three freely available algorithms i.e. BestKeeper, geNorm, and NormFinder. The consensus rankings generated from stability values provided by these programs suggest a combination of at least two genes for normalization. ACT and RPS17 are the most dependably expressed reference genes and therefore, we propose an ACT/RPS17 combination for normalization in all Ae. aegypti derived samples. GAPDH performed least desirably, and is thus not a recommended reference gene. This study emphasizes the importance of validating reference genes in Ae. aegypti for qPCR based research.
    MeSH terms: Aedes/embryology; Aedes/genetics*; Animals; Life Cycle Stages; Reproducibility of Results; Genes, Insect; Gene Expression Regulation, Developmental*; Computational Biology/methods; Gene Expression Profiling; Transcriptome*; Real-Time Polymerase Chain Reaction
  11. Chin PS, Yu CY, Ang GY, Yin WF, Chan KG
    J Glob Antimicrob Resist, 2017 06;9:41-42.
    PMID: 28300643 DOI: 10.1016/j.jgar.2016.12.017
    OBJECTIVES: Salmonella spp. represent one of the main diarrhoeal pathogens that are transmitted via the food supply chain. Here we report the draft genome sequence of a multidrug-resistant Salmonella enterica serovar Brancaster (PS01) that was isolated from poultry meat in Malaysia.

    METHODS: Genomic DNA was extracted from Salmonella strain PS01 and was sequenced using an Illumina HiSeq 2000 platform. The generated reads were de novo assembled using CLC Genomics Workbench. The draft genome was annotated and the presence of antimicrobial resistance genes was identified.

    RESULTS: The 5 036 442bp genome contains various antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, fosfomycin, macrolides, phenicols, sulphonamides, tetracyclines and trimethoprim. The β-lactamase gene blaTEM-176 encoding TEM-176 was also found in this strain.

    CONCLUSIONS: The genome sequence will aid in the understanding of drug resistance mechanisms in foodborne Salmonella Brancaster and highlights the need to ensure the judicious use of antibiotics in animal husbandry as well as the importance of implementing proper food handling and preparation practices.

    MeSH terms: Animals; Chickens; Genes, Bacterial; Malaysia; Meat/microbiology*; Genome*; Sequence Analysis, DNA*; Computational Biology; Salmonella enterica/classification; Salmonella enterica/genetics*; Drug Resistance, Bacterial; Molecular Sequence Annotation; High-Throughput Nucleotide Sequencing; Serogroup
  12. Thevi T, Maizura Z, Abas AL
    Indian J Ophthalmol, 2017 Jan;65(1):24-29.
    PMID: 28300736 DOI: 10.4103/ijo.IJO_452_16
    INTRODUCTION: As in all surgeries, complications during cataract surgery are unavoidable and cause distress to the surgeon and the patient. This study was conducted to know the factors contributing to complications in our settings, to be able to counsel patients about complications and outcomes and to reduce litigations.

    MATERIALS AND METHODS: The secondary data analysis was conducted using the National Eye Database from 2007 to 2014. Demographic features, ocular comorbidities, technique of surgery, grade of surgeons, types of intraoperative complications, and reasons for not obtaining good visual acuity following intraoperative complications were studied. Statistics was done using Statistical Package for Social Sciences version 20.

    RESULTS: Out of 12,992 eyes, 6.1% had intraoperative complications. The highest rate of complications was when more trainees (medical officers [MOs] and gazetting specialists) operated. Posterior capsule rupture (PCR) was the most common complication followed by vitreous loss and zonular dehiscence. Those aged below 40 years had more complications (P < 0.05), and females had more complications. Ethnicity did not affect complications. Pseudoexfoliation was the only comorbidity causing complications (P < 0.05). Phacolytic lenses had 8.118 times the odds of getting intraoperative complications. MOs and gazetting specialists got more complications. Good outcomes were obtained in cases without complications and those operated by specialists. High astigmatism was the main reason for poorer outcomes.

    CONCLUSION: Intraoperative complications were caused mostly by less experienced doctors and had poorer outcomes. Age below 40 years, females, the presence of pseudoexfoliation and phacolytic lenses had more complications. PCR was the most common complication.

    MeSH terms: Adult; Aged; Aged, 80 and over; Cataract Extraction/adverse effects*; Female; Hospitals/statistics & numerical data*; Humans; Intraoperative Complications/epidemiology*; Malaysia/epidemiology; Male; Middle Aged; Postoperative Complications/epidemiology*; Retrospective Studies
  13. Enche Ady CNA, Lim SM, Teh LK, Salleh MZ, Chin AV, Tan MP, et al.
    J Neurosci Res, 2017 Oct;95(10):2005-2024.
    PMID: 28301062 DOI: 10.1002/jnr.24048
    The rapid increase in the older population has made age-related diseases like Alzheimer's disease (AD) a global concern. Given that there is still no cure for this neurodegenerative disease, the drastic growth in the number of susceptible individuals represents a major emerging threat to public health. The poor understanding of the mechanisms underlying AD is deemed the greatest stumbling block against progress in definitive diagnosis and management of this disease. There is a dire need for biomarkers that can facilitate early diagnosis, classification, prognosis, and treatment response. Efforts have been directed toward discovery of reliable and distinctive AD biomarkers but with very little success. With the recent emergence of high-throughput technology that is able to collect and catalogue vast datasets of small metabolites, metabolomics offers hope for a better understanding of AD and subsequent identification of biomarkers. This review article highlights the potential of using multiple metabolomics platforms as useful means in uncovering AD biomarkers from body fluids. © 2017 Wiley Periodicals, Inc.
    MeSH terms: Alzheimer Disease/diagnosis*; Body Fluids/chemistry*; Humans; Biomarkers/analysis*; Metabolomics/methods*
  14. Tan SC, Ismail MP, Duski DR, Othman NH, Bhavaraju VM, Ankathil R
    Cancer Invest, 2017 Mar 16;35(3):163-173.
    PMID: 28301252 DOI: 10.1080/07357907.2017.1278767
    This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.
    MeSH terms: Adult; Carcinoma, Squamous Cell/diagnosis*; Carcinoma, Squamous Cell/genetics; Carcinoma, Squamous Cell/virology; Uterine Cervical Neoplasms/diagnosis*; Uterine Cervical Neoplasms/genetics; Uterine Cervical Neoplasms/virology; Cervix Uteri/metabolism; Female; Humans; Middle Aged; Reference Standards; Gene Expression Profiling/standards; Molecular Diagnostic Techniques/standards*; Papillomavirus Infections/diagnosis*; Papillomavirus Infections/genetics; Papillomavirus Infections/virology; Human papillomavirus 16/physiology*; Transcriptome; Real-Time Polymerase Chain Reaction/standards*
  15. Shadab M, Haque S, Sheshala R, Meng LW, Meka VS, Ali J
    Curr Pharm Des, 2017;23(3):440-453.
    PMID: 27784250 DOI: 10.2174/1381612822666161026163201
    BACKGROUND: The drug delivery of macromolecules such as proteins and peptides has become an important area of research and represents the fastest expanding share of the market for human medicines. The most common method for delivering macromolecules is parenterally. However parenteral administration of some therapeutic macromolecules has not been effective because of their rapid clearance from the body. As a result, most macromolecules are only therapeutically useful after multiple injections, which causes poor compliance and systemic side effects.

    METHOD: Therefore, there is a need to improve delivery of therapeutic macromolecules to enable non-invasive delivery routes, less frequent dosing through controlled-release drug delivery, and improved drug targeting to increase efficacy and reduce side effects.

    RESULT: Non-invasive administration routes such as intranasal, pulmonary, transdermal, ocular and oral delivery have been attempted intensively by formulating macromolecules into nanoparticulate carriers system such as polymeric and lipidic nanoparticles.

    CONCLUSION: This review discusses barriers to drug delivery and current formulation technologies to overcome the unfavorable properties of macromolecules via non-invasive delivery (mainly intranasal, pulmonary, transdermal oral and ocular) with a focus on nanoparticulate carrier systems. This review also provided a summary and discussion of recent data on non-invasive delivery of macromolecules using nanoparticulate formulations.

    MeSH terms: Drug Carriers/administration & dosage; Drug Carriers/chemistry; Drug Compounding; Drug Delivery Systems*; Macromolecular Substances/chemistry; Nanoparticles/administration & dosage; Nanoparticles/chemistry*
  16. Crawford B, Hashim SS, Prepageran N, See GB, Meier G, Wada K, et al.
    Drugs Real World Outcomes, 2017 Mar;4(1):21-31.
    PMID: 27888477 DOI: 10.1007/s40801-016-0099-9
    BACKGROUND: Acute otitis media (AOM) affects both child and parental quality of life (QoL). Data on QoL associated with AOM in Malaysia is sparse, and the burden of indirect costs have not been previously reported.

    OBJECTIVE: To determine the effect of pediatric AOM on child and parental QoL in Malaysia and its economic impact (indirect costs).

    METHODS: We utilized a set of QoL questionnaires (PAR-AOM-QOL, OM-6, and EQ-5D) combined with questions addressing work/productivity loss and financial costs associated with caring for a child during his or her illness in an observational, multicenter, prospective study.

    RESULTS: One hundred and ten AOM patients aged ≤5 years were included in the analysis. The majority of respondents were the patient's mother. Parental QoL was negatively affected for both emotional and daily disturbance scales, but the level of disturbance was low. Using OM-6, the greatest negative impact was on the child's QoL, followed by caregiver concerns, physical suffering, and emotional distress. Using EQ-5D, a moderately positive relationship between parents' emotional disturbance and daily disturbance, and a weak, negative correlation between parental emotional disturbance and parental health status was found. Parents with paid employment took an average of 21 h from work to care for their child, at an average cost of 321.8 Malaysian ringgit (US$97) in addition to their contribution to direct medical costs. Productivity losses whilst at work, uncompensated wage losses, and leisure time losses are also reported.

    CONCLUSIONS: This study found that AOM is associated with some negative impact on parental QoL and significant economic impact at both patient and societal levels. The findings provide useful data on healthcare resource utilization and disease burden of AOM in Malaysia.

    MeSH terms: Child; Child, Preschool; Efficiency; Employment; Female; Health Status; Humans; Leisure Activities; Malaysia; Mothers; Otitis Media; Pain; Prospective Studies; Quality of Life; Surveys and Questionnaires; Salaries and Fringe Benefits; Caregivers
  17. Mustafa KB, Keane KN, Walz NL, Mitrovic KI, Hinchliffe PM, Yovich JL
    Reprod Biol, 2017 Mar;17(1):34-41.
    PMID: 27964842 DOI: 10.1016/j.repbio.2016.11.004
    This seven-year retrospective study analysed the live birth rate (LBR) for women undergoing IVF treatment with various antral follicle counts (AFC). The LBR decreased with lower AFC ratings, and in 290 treatment cycles for women in the poorest AFC category, ≤4 follicles (group E), the LBR was the lowest at 10.7%. The pregnancy loss rate (PLR) significantly increased with poorer AFC categories, from 21.8% in AFC group A (≥20 follicles), to 54.4% in AFC group E (p<0.0001). This trend was repeated with advancing age, from 21.6% for younger women (<35years), to 32.9, 48.5 and 100% for ages 35-39, 40-44 and ≥45 years, respectively (p<0.0001). However, LBR within the specific AFC group E cohort was also age-dependent and decreased significantly from 30.0% for <35 years old, to 13.3, 3.9 and 0% for patients aged 35-39, 40-44 and ≥45 years, respectively. Most, importantly, LBR rates within these age groups were not dependent on the number of IVF attempts (1st, 2nd, 3rd or ≥4 cycles), which indicated that cycle number should not be the primary deciding factor for cessation of IVF treatment in responding women <45years old.
    MeSH terms: Adult; Birth Rate; Corpus Luteum Maintenance/drug effects*; Embryo Transfer*; Female; Fertility Agents, Female/pharmacology; Fertilization in Vitro*; Chorionic Gonadotropin/pharmacology; Humans; Infertility, Female/diagnosis; Infertility, Female/therapy*; Maternal Age; Middle Aged; Ovulation Induction*; Pregnancy; Prognosis; Retrospective Studies; Western Australia/epidemiology; Cohort Studies; Embryo Loss/epidemiology; Embryo Culture Techniques*; Live Birth; Ovarian Reserve*
  18. Harilal M, Vidyadharan B, Misnon II, Anilkumar GM, Lowe A, Ismail J, et al.
    ACS Appl Mater Interfaces, 2017 Mar 29;9(12):10730-10742.
    PMID: 28266837 DOI: 10.1021/acsami.7b00676
    A one-dimensional morphology comprising nanograins of two metal oxides, one with higher electrical conductivity (CuO) and the other with higher charge storability (Co3O4), is developed by electrospinning technique. The CuO-Co3O4 nanocomposite nanowires thus formed show high specific capacitance, high rate capability, and high cycling stability compared to their single-component nanowire counterparts when used as a supercapacitor electrode. Practical symmetric (SSCs) and asymmetric (ASCs) supercapacitors are fabricated using commercial activated carbon, CuO, Co3O4, and CuO-Co3O4 composite nanowires, and their properties are compared. A high energy density of ∼44 Wh kg-1 at a power density of 14 kW kg-1 is achieved in CuO-Co3O4 ASCs employing aqueous alkaline electrolytes, enabling them to store high energy at a faster rate. The current methodology of hybrid nanowires of various functional materials could be applied to extend the performance limit of diverse electrical and electrochemical devices.
  19. Taheri Rouhi SZ, Sarker MMR, Rahmat A, Alkahtani SA, Othman F
    BMC Complement Altern Med, 2017 Mar 14;17(1):156.
    PMID: 28288617 DOI: 10.1186/s12906-017-1667-6
    BACKGROUND: Type 2 diabetes mellitus (T2DM) is associated with hyperglycemia, inflammatory disorders and abnormal lipid profiles. Several functional foods have therapeutic potential to treat chronic diseases including diabetes. The therapeutic potential of pomegranate has been stated by multitudinous scientists. The present study aimed to evaluate the effects of pomegranate juice and seed powder on the levels of plasma glucose and insulin, inflammatory biomarkers, lipid profiles, and health of the pancreatic islets of Langerhans in streptozotocin (STZ)-nicotinamide (NAD) induced T2DM Sprague Dawley (SD) rats.

    METHODS: Forty healthy male SD rats were induced to diabetes with a single dose intra-peritoneal administration of STZ (60 mg/kg b.w.) - NAD (120 mg/kg b.w.). Diabetic rats were orally administered with 1 mL of pomegranate fresh juice (PJ) or 100 mg pomegranate seed powder in 1 mL distilled water (PS), or 5 mg/kg b.w. of glibenclamide every day for 21 days. Rats in all groups were sacrificed on day 22. The obtained data was analyzed by SPSS software (v: 22) using One-way analysis of variance (ANOVA).

    RESULTS: The results showed that PJ and PS treatment had slight but non-significant reduction of plasma glucose concentration, and no impact on plasma insulin compared to diabetic control (DC) group. PJ lowered the plasma total cholesterol (TC) and triglyceride (TG) significantly, and low-density lipoproteins (LDL) non-significantly compared to DC group. In contrast, PS treatment significantly raised plasma TC, LDL, and high-density lipoproteins (HDL) levels compared to the DC rats. Moreover, the administration of PJ and PS significantly reduced the levels of plasma inflammatory biomarkers, which were actively raised in diabetic rats. Only PJ treated group showed significant repairment and restoration signs in islets of Langerhans. Besides, PJ possessed preventative impact against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals almost 2.5 folds more than PS.

    CONCLUSIONS: Our findings suggest that active constituents with high antioxidant properties present in PJ are responsible for its anti-hyperlipidemic and anti-inflammatory effects, likewise the restoration effect on the damaged islets of Langerhans in experimental rats. Hence, the pharmacological, biochemical, and histopathological profiles of PJ treated rats obviously indicated its helpful effects in amelioration of diabetes-associated complications.

    MeSH terms: Fruit and Vegetable Juices/analysis*; Animals; Cholesterol/blood; Diabetes Mellitus, Type 2/blood; Diabetes Mellitus, Type 2/diet therapy*; Diabetes Mellitus, Type 2/drug therapy*; Diabetes Mellitus, Type 2/pathology; Disease Models, Animal; Humans; Hypoglycemic Agents/administration & dosage*; Insulin/blood; Islets of Langerhans/drug effects; Islets of Langerhans/metabolism; Islets of Langerhans/pathology; Lipoproteins/blood; Male; Niacinamide; Plant Extracts/administration & dosage*; Seeds/chemistry; Streptozocin; Triglycerides/blood; Biomarkers/blood; Rats, Sprague-Dawley; Lythraceae/chemistry*; Rats
  20. Ahmed RH, Huri HZ, Muniandy S, Al-Hamodi Z, Al-Absi B, Alsalahi A, et al.
    Clin Biochem, 2017 Sep;50(13-14):746-749.
    PMID: 28288852 DOI: 10.1016/j.clinbiochem.2017.03.008
    OBJECTIVES: Soluble DPP4 (sDPP4) is a novel adipokine that degrades glucagon-like peptide (GLP-1). We evaluated the fasting serum levels of active GLP-1 and sDPP4 in obese, overweight and normal weight subjects to assess the association between sDPP4 levels, active GLP-1 levels and insulin resistance in obese subjects.

    METHODS: The study involved 235 Malaysian subjects who were randomly selected (66 normal weight subjects, 97 overweight, 59 obese subjects, and 13 subjects who were underweight). Serum sDPP4 and active GLP-1 levels were examined by enzyme-linked immunosorbent assay (ELISA). Also, body mass index kg/m(2) (BMI), lipid profiles, insulin and glucose levels were evaluated. Insulin resistance (IR) was estimated via the homeostasis model assessment for insulin resistance (HOMA-IR).

    RESULTS: Serum sDPP4 levels were significantly higher in obese subjects compared to normal weight subjects (p=0.034), whereas serum levels of active GLP-1 were lower (p=0.021). In obese subjects, sDPP4 levels correlated negatively with active GLP-1 levels (r(2)=-0.326, p=0.015). Furthermore, linear regression showed that sDPP4 levels were positively associated with insulin resistance (B=82.28, p=0.023) in obese subjects.

    CONCLUSION: Elevated serum sDPP4 levels and reduced GLP-1 levels were observed in obese subjects. In addition, sDPP4 levels correlated negatively with active GLP-1 levels but was positively associated with insulin resistance. This finding provides evidence that sDPP4 and GLP-1 may play an important role in the pathogenesis of obesity, suggesting that sDPP4 may be valuable as an early marker for the augmented risk of obesity and insulin resistance.

    MeSH terms: Adult; Aged; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Obesity/blood*; Obesity/ethnology; Obesity/metabolism; Obesity/epidemiology; Risk; Solubility; Thinness/blood; Thinness/ethnology; Thinness/metabolism; Thinness/epidemiology; Biomarkers/blood; Down-Regulation; Up-Regulation; Body Mass Index; Linear Models; Dipeptidyl Peptidase 4/blood*; Dipeptidyl Peptidase 4/chemistry; Overweight/blood*; Overweight/ethnology; Overweight/metabolism; Overweight/epidemiology; Glucagon-Like Peptide 1/blood*
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