Browse publications by year: 2019

  1. Jajere SM, Hassan L, Abdul Aziz S, Zakaria Z, Abu J, Nordin F, et al.
    Poult Sci, 2019 Nov 01;98(11):5961-5970.
    PMID: 31392329 DOI: 10.3382/ps/pez392
    Village chicken or Ayam Kampung, common to Southeast Asian countries, has always been regarded as superior in comparison to commercial broiler chicken in terms of wholesomeness and health benefits. The current study investigates the prevalence and risk factors of Salmonella among village chicken flocks from the central and southern states of Peninsular Malaysia. A total of 35 village flocks were sampled from Selangor (n = 19), Melaka (n = 10), Johor (n = 4), and Negeri Sembilan (n = 2). In total, 1,042 samples were collected; these included cloacal swabs (n = 675), eggs (n = 62), pooled drinking water (n = 175), pooled feeds (n = 70), and pooled flies (n = 60). Isolation of Salmonella from cloacal swabs, poultry drinking water, and feeds was carried out according to the protocols and recommendations of the World Organization for Animal Health (OIE) terrestrial manual. The prevalence of Salmonella at an individual bird-level was 2.5% (17/675, 95% CI: 1.6 to 4.0). All eggs screened were negative; in the case of environmental samples, however, Salmonella was detected in 5.14% (9/175), 7.14% (5/70), and 5.0% (3/60) for water, feed, and flies, respectively. A total of 34 isolates and 8 Salmonella serotypes were identified. Weltevreden (20.6%) was the most common, followed by Typhimurium and Agona (17.6%), Albany and Enteritidis (8.8%), Molade (5.9%), Corvallis and Schleissheim (2.9%), and others grouped as Salmonella spp. (11.8%). Multivariable logistic regression models revealed that Salmonella positivity among flocks could be strongly predicted by storage of feeds (uncovered feeds; OR = 10.38; 95% CI: 1.25 to 86.39; p = 0.030) and uncovered water tanks (uncovered tank; OR = 6.43; 95% CI: 1.02 to 40.60; p = 0.048). The presence of Salmonella in village chickens in the study area was lower than that of commercial chickens in Malaysia.
    MeSH terms: Animals; Chickens; Malaysia/epidemiology; Poultry Diseases/microbiology; Poultry Diseases/epidemiology*; Risk Factors; Salmonella/isolation & purification*; Salmonella Infections, Animal/microbiology; Salmonella Infections, Animal/epidemiology*; Prevalence; Farms
  2. Sahudin MA, Su'ait MS, Tan LL, Lee YH, Abd Karim NH
    Anal Bioanal Chem, 2019 Sep;411(24):6449-6461.
    PMID: 31392436 DOI: 10.1007/s00216-019-02025-4
    Biogenic amines have attracted interest among researchers because of their importance as biomarkers in determining the quality of food freshness in the food industry. A rapid and simple technique that is able to detect biogenic amines is needed. In this work, a new optical sensing material for one of the biogenic amines, histamine, based on a new zinc(II) salphen complex was developed. The binding of zinc(II) complexes without an electron-withdrawing group (complex 1) and with electron-withdrawing groups (F, complex 2; Cl, complex 3) to histamine resulted in enhancement of fluorescence. All complexes exhibited high affinity for histamine [binding constant of (7.14 ± 0.80) × 104, (3.33 ± 0.03) × 105, and (2.35 ± 0.14) × 105 M-1, respectively]. Complex 2 was chosen as the sensing material for further development of an optical sensor for biogenic amines in the following step since it displayed enhanced optical properties in comparison with complexes 1 and 3. The optical sensor for biogenic amines used silica microparticles as the immobilisation support and histamine as the analyte. The optical sensor had a limit of detection for histamine of 4.4 × 10-12 M, with a linear working range between 1.0 × 10-11 and 1.0 × 10-6 M (R2 = 0.9844). The sensor showed good reproducibility, with a low relative standard deviation (5.5 %). In addition, the sensor exhibited good selectivity towards histamine and cadaverine over other amines, such as 1,2-phenylenediamine, triethylamine, and trimethylamine. Recovery and real sample studies suggested that complex 2 could be a promising biogenic amine optical sensing material that can be applied in the food industry, especially in controlling the safety of food for it to remain fresh and healthy for consumption.
    MeSH terms: Biogenic Amines/analysis*; Fluorometry/methods*; Histamine/analysis; Phenylenediamines/chemistry*; Spectrophotometry, Ultraviolet/instrumentation*; Reproducibility of Results; Zinc Compounds/chemistry*; Limit of Detection
  3. Rajendiran T, Sabarathinam C, Chandrasekar T, Keesari T, Senapathi V, Sivaraman P, et al.
    Environ Sci Pollut Res Int, 2019 Oct;26(28):29173-29190.
    PMID: 31392611 DOI: 10.1007/s11356-019-05962-w
    This study considered the temporal variations in rainfall and water level patterns as governing factors, which influence the geochemical process of coastal aquifer around Pondicherry, South India. Rainfall and water level data were collected from 2006 to 2016, which showed that the amount of rainfall from 2006 to 2011 was higher than that of 2011 to 2016. To understand the geochemical process governing groundwater, samples were collected during 2006 (n = 54), followed by 2011 (n = 93), and during 2016 (n = 63) as part of continuous observation. The major ions and stable isotopes (δ18O and δD) were analyzed in the samples to determine the geochemical variations. The predominant types were noted as Na-HCO3 and Na-Cl; Ca-HCO3 and Ca-Mg-Cl; and Na-Cl and Ca-Mg-Cl in 2006, 2011, and 2016, respectively. Saturation states of sulfate and carbonate minerals were compared for the study periods and it indicates that the saturation index (SI) values were increased from 2006 to 2011, but decreased from 2011 to 2016. PHREEQC inverse modeling revealed the predominance for the dissolution and leaching of carbonate minerals during increased rainy periods, and the increase of halite saturation during lesser rainfall period. AQUACHEM mixing studies suggested that geochemical signatures of 2006 and 2011 were preserved in samples of 2016 in different proportions. Considering the major factors, the main processes prevailing in the study area were inferred to be dissolution and leaching during 2006~2011 years and seawater intrusion along with ion exchange during 2011~2016 years. In all these periods of study, anthropogenic impact was also identified in the groundwater samples. Hence, this study revealed that the rainfall and water level gave a significant variation in the geochemical process of groundwater in the coastal aquifer system.
    MeSH terms: Deuterium/analysis; Environmental Monitoring; India; Ion Exchange; Oxygen Isotopes/analysis; Rain; Seawater; Groundwater/analysis*; Groundwater/chemistry*
  4. Lee BW, Park JG, Ha TKQ, Pham HTT, An JP, Noh JR, et al.
    J Nat Prod, 2019 08 23;82(8):2201-2210.
    PMID: 31393125 DOI: 10.1021/acs.jnatprod.9b00224
    Melicope pteleifolia has long been consumed as a popular vegetable and tea in Southeast Asian countries, including Malaysia and southern mainland China, and is effective in the treatment of colds and inflammation. In the search for active metabolites that can explain its traditional use as an antipyretic, six new phloroacetophenone derivatives (3-8) along with seven known compounds (1, 2, and 9-13) were isolated from the leaves of M. pteleifolia. Their chemical structures were confirmed by extensive spectroscopic analysis including NMR, IR, ECD, and HRMS. All compounds isolated from the leaves of M. pteleifolia (1-13) have a phloroacetophenone skeleton. Notably, the new compound 8 contains an additional cyclobutane moiety in its structure. The bioactivities of the isolated compounds were evaluated, and compounds 1, 6, and 7 inhibited tumor necrosis factor-α-induced prostaglandin E2. Moreover, the major constituent, 3,5-di-C-β-d-glucopyranosyl phloroacetophenone (1), was found to be responsible for the antipyretic activity of M. pteleifolia based on in vivo experiments.
    MeSH terms: Analgesics/pharmacology*; Animals; Cells, Cultured; Humans; Male; Mice, Inbred ICR; Plants, Edible/chemistry; Spectrum Analysis/methods; Molecular Structure; Plant Leaves/chemistry*; Rutaceae/chemistry*; Mice
  5. Zafar MN, Masood S, Chaudhry GE, Muhammad TST, Dalebrook AF, Nazar MF, et al.
    Dalton Trans, 2019 Aug 08.
    PMID: 31393494 DOI: 10.1039/c9dt01923e
    The two cationic palladium(ii) complexes, [Pd(Len)2][OTf]2 (4) and [Pd(Lphen)2][OTf]2 (5), were synthesized by treatment of bis(benzonitrile)dichloropalladium(ii) with [H2Len][OTf]2 (2) or [H2Lphen][OTf]2 (3), respectively, in the presence of a weak base. The pro-ligands 2 and 3 were synthesized by melt reactions between N-methyl-4-chloropyridinium triflate (1) and the amines ethylenediamine or phenylenediamine, respectively. The water-soluble compounds 2-5 were fully characterized, including by single-crystal X-ray crystal structure determinations for 2-4. UV-Vis and fluorescence spectroscopy were used to study the binding interactions of 2-5 with CT-DNA. The spectroscopic data suggested the presence of intercalative and groove binding modes and this was supported by molecular docking studies. The in vitro cytotoxicity studies (IC50 values) showed that the human breast cancer cell lines MCF-7 and T47D were more sensitive towards 3, 4 and 5 than cisplatin. The cytotoxicity of the new compounds decreased in the order 5 > 4 > 3 > 2. Furthermore, the annexin V-FITC staining method strongly suggested the presence of phosphatidylserine (PS) on the outer membrane of the treated cells, which is a hallmark of apoptosis.
    MeSH terms: Amines; Breast Neoplasms; Cisplatin; DNA; Ethylenediamines; Humans; Ligands; Nitriles; Palladium; Phenylenediamines; Phosphatidylserines; Spectrometry, Fluorescence; Staining and Labeling; Tomography, X-Ray Computed; Water; X-Rays; Fluorescein-5-isothiocyanate; Apoptosis; Annexin A5; Inhibitory Concentration 50; MCF-7 Cells; Molecular Docking Simulation
  6. Yusof S, Mohamad Dzomir AZ, Yaakop S
    J Econ Entomol, 2019 12 09;112(6):2808-2816.
    PMID: 31393572 DOI: 10.1093/jee/toz217
    The aim of this study was to investigate the effect of gamma irradiation on survivability (adult emergence, sex ratio, adult longevity), fecundity (eggs hatchability, number of eggs produced), and morphological differences in the size of the ovary and testes of unirradiated and irradiated adults of Oriental fruit fly [Bactrocera dorsalis (Hendel)]. A dose of 100 Gy was determined as the minimum needed for inhibitory effects against B. dorsalis that would not deter adult emergence, sex ratio (male:female), and adult longevity, with 82.6 ±7.02, 1:1.09, and 107 ± 24.5, respectively. Doses from 50 to 400 Gy range did not completely prevent the adult emergence; however, the emergence decreased by increasing the radiation dose. Adult survivability significantly decreased among all the treatment groups of B. dorsalis, except for the 50 Gy and unirradiated flies. At a dose of 50 Gy, fertile females showed a significant reduction in fecundity by not producing eggs after mating with the sterile males. Meanwhile, the number of eggs laid decreased with increasing dose and no fertile egg was hatched starting at 100 Gy of irradiation. Testes and ovaries of 20-d old flies irradiated as pupae were smaller than those of control flies. In this study, 100 Gy was concluded as the minimum effective dose for the disinfestation and sterilization of B. dorsalis puparia. Results represent new findings used as a basis for sterile insect technique and quarantine programs for managing B. dorsalis, particularly in Malaysia.
    MeSH terms: Animals; Female; Fertility; Malaysia; Male; Pupa; Quarantine; Tephritidae*
  7. Łuniewska M, Wodniecka Z, Miller CA, Smolík F, Butcher M, Chondrogianni V, et al.
    PLoS One, 2019;14(8):e0220611.
    PMID: 31393919 DOI: 10.1371/journal.pone.0220611
    We present a new set of subjective Age of Acquisition (AoA) ratings for 299 words (158 nouns, 141 verbs) in seven languages from various language families and cultural settings: American English, Czech, Scottish Gaelic, Lebanese Arabic, Malaysian Malay, Persian, and Western Armenian. The ratings were collected from a total of 173 participants and were highly reliable in each language. We applied the same method of data collection as used in a previous study on 25 languages which allowed us to create a database of fully comparable AoA ratings of 299 words in 32 languages. We found that in the seven languages not included in the previous study, the words are estimated to be acquired at roughly the same age as in the previously reported languages, i.e. mostly between the ages of 1 and 7 years. We also found that the order of word acquisition is moderately to highly correlated across all 32 languages, which extends our previous conclusion that early words are acquired in similar order across a wide range of languages and cultures.
    MeSH terms: Age Factors; Child; Child, Preschool; Culture; Humans; Infant; Language*; Learning; Vocabulary*
  8. Latif MT, Abd Hamid HH, Ahamad F, Khan MF, Mohd Nadzir MS, Othman M, et al.
    Chemosphere, 2019 Dec;237:124451.
    PMID: 31394440 DOI: 10.1016/j.chemosphere.2019.124451
    This study aims to determine the composition of BTEX (benzene, toluene, ethylbenzene and xylene) and assess the risk to health at different sites in Malaysia. Continuous monitoring of BTEX in Kuala Lumpur City Centre, Kuala Terengganu, Kota Kinabalu and Fraser Hill were conducted using Online Gas Chromatograph. For comparison, BTEX at selected hotspot locations were determined by active sampling method using sorbent tubes and Thermal Desorption Gas Chromatography Mass Spectrometry. The hazard quotient (HQ) for non-carcinogenic and the life-time cancer risk (LTCR) of BTEX were calculated using the United States Environmental Protection Agency (USEPA) health risk assessment (HRA) methods. The results showed that the highest total BTEX concentrations using continuous monitoring were recorded in the Kuala Lumpur City Centre (49.56 ± 23.71 μg/m3). Toluene was the most dominant among the BTEX compounds. The average concentrations of benzene ranged from 0.69 ± 0.45 μg/m3 to 6.20 ± 3.51 μg/m3. Measurements using active sampling showed that BTEX concentrations dominated at the roadside (193.11 ± 114.57 μg/m3) in comparison to petrol station (73.08 ± 30.41 μg/m3), petrochemical industry (32.10 ± 13.13 μg/m3) and airport (25.30 ± 6.17 μg/m3). Strong correlations among BTEX compounds (p<0.01, r>0.7) at Kuala Lumpur City Centre showed that BTEX compounds originated from similar sources. The values of HQ at all stations were <1 indicating the non-carcinogenic risk are negligible and do not pose threats to human health. The LTCR value based on benzene inhalation (1.59 × 10-5) at Kuala Lumpur City Centre were between 1 × 10-4 and 1 × 10-5, representing a probable carcinogenic risk.
    MeSH terms: Benzene/analysis*; Benzene/toxicity; Benzene Derivatives/analysis*; Benzene Derivatives/toxicity; Carcinogens/analysis; Carcinogens/toxicity; Environmental Monitoring/methods*; Humans; Malaysia; Gas Chromatography-Mass Spectrometry; Toluene/analysis*; Toluene/toxicity; Xylenes/analysis*; Xylenes/toxicity; Risk Assessment*
  9. Aftab RA, Khan AH, Adnan AS, Sulaiman SAS, Khan TM
    Blood Purif., 2019;48(3):233-242.
    PMID: 31387098 DOI: 10.1159/000500997
    AIMS AND OBJECTIVE: To estimate the effect of losartan 50 mg on survival of post-dialysis euvolemic hypertensive patients.

    METHODOLOGY: A single center, prospective, single-blind randomized trial was conducted to estimate the survival of post-dialysis euvolemic hypertensive patients when treated with lorsartan 50 mg every other day. Post-dialysis euvolemic assessment was done by a body composition monitor. Covariate Adaptive Randomization was used for allocation of participants to the standard or intervention arm, and the follow-up duration was twelve months. The primary end point was achieving targeted blood pressure (BP) of <140/90 mm Hg and maintaining for 4 weeks, whereas secondary end point was all cause of mortality. Pre-, intra-, and post-dialysis session BP measurements were recorded, and survival trends were analyzed using Kaplan-Meier analysis.

    RESULTS: Of the total 229 patients, 96 (41.9%) were identified as post-dialysis euvolemic hypertensive. Final samples of 88 (40.1%) patients were randomized into standard (n = 44) and intervention arms (n = 44), and 36 (81.8%) patients in each arm completed a follow-up of 12 months. A total of eight patients passed away during the 12-month follow-up period (6 deaths among standard arm and 2 in intervention arm). However, the probability of survival between both arms was not significant (p = 0.13). Cox regression analysis revealed that chances of survival were higher among the patients in the intervention (OR 3.17) arm than the standard arm (OR 0.31); however, the survival was found not statistically significant.

    CONCLUSION: There was no statistical significant difference in 1 year survival of post-dialysis euvolemic hypertensive patients when treated with losartan 50 mg.

    MeSH terms: Adult; Aged; Antihypertensive Agents/administration & dosage; Antihypertensive Agents/therapeutic use; Blood Pressure; Female; Follow-Up Studies; Renal Dialysis; Humans; Hypertension/drug therapy*; Hypertension/mortality; Male; Middle Aged; Survival Analysis; Treatment Failure; Losartan/administration & dosage*; Losartan/pharmacology; Losartan/therapeutic use
  10. Harapan H, Rajamoorthy Y, Utomo PS, Anwar S, Setiawan AM, Alleta A, et al.
    BMC Infect Dis, 2019 Aug 06;19(1):693.
    PMID: 31387537 DOI: 10.1186/s12879-019-4297-4
    BACKGROUND: The aim of this study was to assess the knowledge and attitudes towards pregnancy-related issues of Zika virus (ZIKV) infection among general practitioners (GPs), a frontline healthcare worker group, in Indonesia.

    METHODS: A cross-sectional, online survey assessing knowledge and attitudes towards ZIKV infection on multiple-item scales was sent to GPs in the Sumatra and Java islands of Indonesia. The associations between independent factors and either knowledge or attitude were assessed with logistic regressions. The correlation and association between knowledge and attitude were estimated.

    RESULTS: We included 457 (53.7%) out of 850 responses in the analysis. Among these, 304 (66.5%) and 111 (24.2%) respondents had a good knowledge and attitude, respectively. No demographic, workplace, professional development, or experiential characteristics related to ZIKV infection were associated with knowledge. In the multivariate analysis, only contact experience was associated with attitude. There was a significant, positive correlation between knowledge and attitude scores.

    CONCLUSIONS: Although knowledge of pregnancy-related complications of ZIKV infection is relatively high among GPs in Indonesia, more than 75% of them had a poor attitude towards pregnancy-related issues of Zika. Strategies for enhancing the capacity of GPs to develop positive attitudes and respond to ZIKV infection are needed.

    MeSH terms: Zika Virus Infection/etiology*; Adult; Cross-Sectional Studies; Female; Health Surveys; Humans; Indonesia; Health Knowledge, Attitudes, Practice*; Male; Pregnancy; Pregnancy Complications, Infectious/virology*; Multivariate Analysis; General Practitioners*
  11. Hoshi T, Brugman VA, Sato S, Ant T, Tojo B, Masuda G, et al.
    Sci Rep, 2019 08 06;9(1):11412.
    PMID: 31388090 DOI: 10.1038/s41598-019-47511-y
    Mosquito surveillance is a fundamental component of planning and evaluating vector control programmes. However, logistical and cost barriers can hinder the implementation of surveillance, particularly in vector-borne disease-endemic areas and in outbreak scenarios in remote areas where the need is often most urgent. The increasing availability and reduced cost of 3D printing technology offers an innovative approach to overcoming these challenges. In this study, we assessed the field performance of a novel, lightweight 3D-printed mosquito light trap baited with carbon dioxide (CO2) in comparison with two gold-standard traps, the Centers for Disease Control and Prevention (CDC) light trap baited with CO2, and the BG Sentinel 2 trap with BG-Lure and CO2. Traps were run for 12 nights in a Latin square design at Rainham Marshes, Essex, UK in September 2018. The 3D-printed trap showed equivalent catch rates to the two commercially available traps. The 3D-printed trap designs are distributed free of charge in this article with the aim of assisting entomological field studies across the world.
    MeSH terms: Aedes*; Animals; Carbon Dioxide/chemistry; Equipment Design; Great Britain; Light*; Software; Ecological Parameter Monitoring/economics; Ecological Parameter Monitoring/instrumentation*; Printing, Three-Dimensional/economics*; Mosquito Vectors*
  12. Jairoun A, Hassan N, Ali A, Jairoun O, Shahwan M, Hassali M
    Int J Gen Med, 2019;12:235-246.
    PMID: 31388309 DOI: 10.2147/IJGM.S200641
    Purpose: Antibiotic resistance is one of the world's most fatal health crises. Medical students are the antibiotic prescribers of tomorrow, so better understanding of their knowledge, attitudes, and practice (KAP) pertaining to antibiotic use is crucial. Objectives: This study aimed to assess the KAP of antibiotics and associated factors among university students in the United Arab Emirates (UAE). Methods: This was a descriptive cross-sectional study conducted among a random sample of undergraduate students from Ajman University in the UAE. A self-administered pretested questionnaire was used to collect data on students' demographics and their KAP regarding antibiotic use. Data were analysed using STATA version 14.2. P<0.05 was considered statistically significant. Results: This study showed that university students have a high rate of antibiotic self-medication. The average KAP score was 56% (95% CI 55%-57%). Statistical modeling showed that major, study year, age, and sex were strong determinants of KAP regarding antibiotic use. Conclusion: There is a need to develop an effective and comprehensive antibiotic-stewardship program as part of undergraduate education. Moreover, reinforcing antibiotic-use policies, involving pharmacies, drug supply, distribution, and sale, are also urgently needed.
    MeSH terms: Anti-Bacterial Agents; Attitude; Cross-Sectional Studies; Demography; Drug Resistance, Microbial; Humans; Pharmacies; Surveys and Questionnaires; Self Medication; Students, Medical; United Arab Emirates; Universities
  13. Norhazrina N, Syazwana N, Aisyah M, Aznani H, Maideen H, M S Nizam
    PhytoKeys, 2019;128:57-72.
    PMID: 31388328 DOI: 10.3897/phytokeys.128.33860
    Gunung Senyum Recreational Forest harbours 59 species, two subspecies and five varieties of mosses in 32 genera and 16 families that had been identified from a total of 589 specimens collected from the area. These figures represent 11.8% out of the 558 taxa, 20.2% out of the 158 genera and 34.7% out of the 46 families of mosses reported for Peninsular Malaysia. The total also represents 14.9% of the 442 taxa, 24.0% of the 133 genera and 40.0% of the 40 families of mosses recorded in Pahang. The largest family of mosses found in this limestone forest is Calymperaceae followed by Fissidentaceae. There are two new records for Pahang, Calymperespallidum Mitt. and Taxitheliumbinsteadii Broth. & Dixon. The analysis of species similarities of mosses found in the study area with some other selected areas showed that Gunung Senyum Recreational Forest had a high percentage of species similarity with Perlis State Park at Wang Kelian, another limestone forest, at 38%. Corticol is the main habitat utilised by mosses in Gunung Senyum Recreational Forest with 47 taxa, followed by the lignicol and calcicol each with 35 and 26 taxa, respectively.
    MeSH terms: Calcium Carbonate; Malaysia; Recreation; Ecosystem; Bryopsida; Bryophyta; Forests
  14. Abd Manan TSB, Beddu S, Khan T, Wan Mohtar WHM, Sarwono A, Jusoh H, et al.
    MethodsX, 2019;6:1701-1705.
    PMID: 31388505 DOI: 10.1016/j.mex.2019.07.011
    Polycyclic aromatic hydrocarbons (PAHs) are carcinogenic compounds, composed of two or more fused benzene rings and abundantly found in mixed-use areas. Mixed-use areas consist of dense population, urbanization, industrial and agricultural activities. River pollution are common in mixed-use areas and 98% of Malaysia's fresh water supply originates from surface water. The biological degradation, adsorption and advanced oxidation process were documented as the available PAHs treatment for water. To date, the application of the photo-Fenton oxidation process has been reported for the treatment of PAHs from contaminated soil (review paper), landfill leachate, municipal solid waste leachate, sanitary landfill leachate, aniline wastewater, ammunition wastewater and saline aqueous solutions. As for potable water, the application of Fenton reagent was aided with photo treatment or electrolysis not focusing on PAHs removal. •The presented MethodsX was conducted for PAHs degradation analysis in potable water samples using photo-Fenton oxidation process.•The designed reactor for batch experiment is presented.•The batch experiment consists of parameters like concentration of 17 USEPA-PAHs in the prepared aqueous solution (fixed variable), reaction time, pH and molarity ratio of hydrogen peroxide (H2O2): ferrous sulfate (FeSO4).
  15. Kang IN, Lee CY, Tan SC
    Heliyon, 2019 Jul;5(7):e02156.
    PMID: 31388587 DOI: 10.1016/j.heliyon.2019.e02156
    Whilst the potential of neural stem cell (NSC)-based treatment is recognized worldwide and seems to offer a promising therapeutic option for stroke treatments, there is currently no full understanding regarding the effects of hypoxic and baicalein-enriched fraction (BEF) preconditioning approaches on the therapeutic potential of these cells for stroke. The potential of preconditioned NSC can be determined based on the expression of several key neuroprotective genes using qRT-PCR technique. However, prior to that, it is imperative and extremely important to carefully select reference gene(s) for accurate qRT-PCR data normalization to avoid error in data interpretation. This study aimed to evaluate the stability of ten candidate reference genes via comprehensive analysis using three algorithms software: geNorm, NormFinder and BestKeeper. Our results revealed that HPRT1 and RPL13A were the most reliable reference genes for BEF-preconditioned NSCs, but ironically, HPRT1 was ranked as the least stable reference gene for hypoxic-preconditioned NSCs. On the other hand, RPLP1 and RPL13A were selected as the most stably expressed pair of reference genes for hypoxic-preconditioned NSCs. In conclusion, this study has pointed out the importance of identifying valid reference genes and has presented the first significant validation on best reference genes recommended for qRT-PCR study involves NSC preconditioned with hypoxia or with BEF extracted from Oroxylum indicum medicinal plant.
  16. Yarmolinsky J, Relton CL, Lophatananon A, Muir K, Menon U, Gentry-Maharaj A, et al.
    PLoS Med, 2019 Aug;16(8):e1002893.
    PMID: 31390370 DOI: 10.1371/journal.pmed.1002893
    BACKGROUND: Various risk factors have been associated with epithelial ovarian cancer risk in observational epidemiological studies. However, the causal nature of the risk factors reported, and thus their suitability as effective intervention targets, is unclear given the susceptibility of conventional observational designs to residual confounding and reverse causation. Mendelian randomization (MR) uses genetic variants as proxies for risk factors to strengthen causal inference in observational studies. We used MR to evaluate the association of 12 previously reported risk factors (reproductive, anthropometric, clinical, lifestyle, and molecular factors) with risk of invasive epithelial ovarian cancer, invasive epithelial ovarian cancer histotypes, and low malignant potential tumours.

    METHODS AND FINDINGS: Genetic instruments to proxy 12 risk factors were constructed by identifying single nucleotide polymorphisms (SNPs) that were robustly (P < 5 × 10-8) and independently associated with each respective risk factor in previously reported genome-wide association studies. These risk factors included genetic liability to 3 factors (endometriosis, polycystic ovary syndrome, type 2 diabetes) scaled to reflect a 50% higher odds liability to disease. We obtained summary statistics for the association of these SNPs with risk of overall and histotype-specific invasive epithelial ovarian cancer (22,406 cases; 40,941 controls) and low malignant potential tumours (3,103 cases; 40,941 controls) from the Ovarian Cancer Association Consortium (OCAC). The OCAC dataset comprises 63 genotyping project/case-control sets with participants of European ancestry recruited from 14 countries (US, Australia, Belarus, Germany, Belgium, Denmark, Finland, Norway, Canada, Poland, UK, Spain, Netherlands, and Sweden). SNPs were combined into multi-allelic inverse-variance-weighted fixed or random effects models to generate effect estimates and 95% confidence intervals (CIs). Three complementary sensitivity analyses were performed to examine violations of MR assumptions: MR-Egger regression and weighted median and mode estimators. A Bonferroni-corrected P value threshold was used to establish strong evidence (P < 0.0042) and suggestive evidence (0.0042 < P < 0.05) for associations. In MR analyses, there was strong or suggestive evidence that 2 of the 12 risk factors were associated with invasive epithelial ovarian cancer and 8 of the 12 were associated with 1 or more invasive epithelial ovarian cancer histotypes. There was strong evidence that genetic liability to endometriosis was associated with an increased risk of invasive epithelial ovarian cancer (odds ratio [OR] per 50% higher odds liability: 1.10, 95% CI 1.06-1.15; P = 6.94 × 10-7) and suggestive evidence that lifetime smoking exposure was associated with an increased risk of invasive epithelial ovarian cancer (OR per unit increase in smoking score: 1.36, 95% CI 1.04-1.78; P = 0.02). In analyses examining histotypes and low malignant potential tumours, the strongest associations found were between height and clear cell carcinoma (OR per SD increase: 1.36, 95% CI 1.15-1.61; P = 0.0003); age at natural menopause and endometrioid carcinoma (OR per year later onset: 1.09, 95% CI 1.02-1.16; P = 0.007); and genetic liability to polycystic ovary syndrome and endometrioid carcinoma (OR per 50% higher odds liability: 0.89, 95% CI 0.82-0.96; P = 0.002). There was little evidence for an association of genetic liability to type 2 diabetes, parity, or circulating levels of 25-hydroxyvitamin D and sex hormone binding globulin with ovarian cancer or its subtypes. The primary limitations of this analysis include the modest statistical power for analyses of risk factors in relation to some less common ovarian cancer histotypes (low grade serous, mucinous, and clear cell carcinomas), the inability to directly examine the association of some ovarian cancer risk factors that did not have robust genetic variants available to serve as proxies (e.g., oral contraceptive use, hormone replacement therapy), and the assumption of linear relationships between risk factors and ovarian cancer risk.

    CONCLUSIONS: Our comprehensive examination of possible aetiological drivers of ovarian carcinogenesis using germline genetic variants to proxy risk factors supports a role for few of these factors in invasive epithelial ovarian cancer overall and suggests distinct aetiologies across histotypes. The identification of novel risk factors remains an important priority for the prevention of epithelial ovarian cancer.

    MeSH terms: Age Factors; Female; Humans; Menarche; Menopause; Ovarian Neoplasms/etiology*; Ovarian Neoplasms/genetics; Parity; Risk Factors; Smoking/adverse effects; Body Mass Index; Genetic Predisposition to Disease/genetics; Mendelian Randomization Analysis
  17. Sultana S, Hossain Z
    Dental Press J Orthod, 2019 Aug 01;24(3):44.e1-44.e9.
    PMID: 31390447 DOI: 10.1590/2177-6709.24.3.44.e1-9.onl
    OBJECTIVE: The purpose of the present study was to assess the prevalence of normative and perceived orthodontic treatment need in schoolchildren and adolescents, related risk factors, and children/parent's aesthetic perception, compared to orthodontist's opinion, in Dhaka city, Bangladesh.

    METHODS: A random sample of 800 schoolchildren aging 11-15 years was selected from different schools in the city of Dhaka, Bangladesh. The Dental Health Component (DHC) and Aesthetic Component (AC) of the Index of Orthodontic Treatment Need (IOTN) were assessed as normative treatment need. The Decayed, Missing, Filled Teeth (DMFT) index was used to record caries experience. Children were interviewed on the perception of orthodontic treatment need. Parents also completed a questionnaire on the perception of their child's orthodontic treatment need, assessed by AC/ IOTN.

    RESULTS: According to the DHC/IOTN, only 24.7% were in the category of definite need (grade 4-5) for orthodontic treatment. A significant difference was found between the clinician/children and clinician/parents perceived AC score of IOTN (p= 0.0001). Multiple logistic regression showed children with a higher DMFT were significantly more likely to need orthodontic treatment, according to the DHC of IOTN.

    CONCLUSION: A low proportion of schoolchildren needs normative orthodontic treatment in the city of Dhaka, Bangladesh. Children with a higher DMFT score were significantly more likely to need orthodontic treatment, according to the DHC of IOTN.

    MeSH terms: Adolescent; Bangladesh; Child; Esthetics, Dental*; Humans; Malocclusion*; Orthodontics, Corrective; Prevalence; Index of Orthodontic Treatment Need
  18. Ho, L.H., Zainal Abidin, N.F.S., Tan, T.C., Noroul Asyikeen, Z.
    MyJurnal
    The effect of partial substitution of pumpkin flour for rice flour on the physical properties and sensory attributes of gluten-free muffin were investigated. Pumpkin flour was used to replace 10, 15 and 20% rice flour in a control gluten-free muffin formulation (without pumpkin flour). The partial substitution of pumpkin flour for rice flour did not affect moisture content of gluten-free muffins. However, the pumpkin flour substitution caused significant reduction in water activity of gluten-free muffins. Results on the volume, specific volume and height of all gluten-free muffins showed no significant effect with the increasing percentage of pumpkin flour substitution. However, pumpkin flour substitution significantly reduced the firmness of composite muffins, and improved its springiness. The colour of crumb progressively became darker as the level of pumpkin flour substitution increased. Moreover, the results also showed that the substitution of pumpkin flour caused an increase in yellowness (b*) value of crust and crumb of gluten-free muffin. Sensory evaluation indicated that all gluten-free muffins incorporated with pumpkin flour received similar score when compared to that of control.
    MeSH terms: Bread; Color; Flour; Glutens; Oryza; Water; Cucurbita; Diet, Gluten-Free; Physical Phenomena
  19. Rahim, N.A., Sarbon, N.M.
    MyJurnal
    The present work was aimed to determine the physicochemical and sensory properties of Acacia honey lime ice cream incorporated with various types of hydrocolloids, including guar gum (GG), xanthan gum (XG), and carboxymethyl cellulose (CMC). The overrun, melting rate, hardness, colour, total soluble solid, microstructure, moisture content, pH, and sensory acceptability of the ice creams produced were characterised. The addition of 15% Acacia honey to the ice cream led to improvements in several characteristics as the hydrocolloids were incorporated. There were no significant differences in terms of total soluble solids, lightness, yellowness, pH and moisture content among all ice cream formulations. However, the overrun, melting rate, hardness, and redness values of ice cream experienced significant changes as hydrocolloids were added. Ice cream incorporated with GG had the highest overrun (9.30%), melting rate (48.33 mL/min) and a* (-1.68 ± 0.13) values. Meanwhile, ice cream incorporated with CMC was higher in hardness (1729.30 g), but lowest in terms of overrun (5.00%), melting rate (28.33 mL/min) and a* (-2.03 ± 0.35) values. An examination of the microstructure found differences in air cell sizes at the interfaces of different types of hydrocolloids. Sensory acceptability showed a significant difference between GG and other formulations. In conclusion, CMC in Acacia honey lime ice cream led to strong improvements in its physicochemical properties.
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