Browse publications by year: 2024

  1. Ten KE, Rahman S, Tan HS
    Microb Genom, 2024 Nov;10(11).
    PMID: 39565092 DOI: 10.1099/mgen.0.001327
    Despite being a major human pathogen, limited studies have reported RNA modifications in Acinetobacter baumannii. These post-transcriptional modifications play crucial regulatory roles in bacteria and have also been shown to modulate bacterial virulence. Using nanopore sequencing, we characterized RNA modifications in a virulent A. baumannii strain (Ab-C98) under free-living (mid-exponential phase in vitro culture) and during an early stage of infection (3 h post-infection) in Galleria mellonella larvae. Analysis revealed that m5C methylations are essential for ribosome synthesis, while m6A and Ψ are involved in metabolic pathways and translation processes. Iron-chelating genes exbD (m5C and m6A) and feoB (m6A and Ψ) and RNA polymerase subunit rpoC (m6A and Ψ) were selectively modified during infection. This first transcriptome-wide study highlights the potential regulatory roles of m5C, m6A and Ψ modifications in A. baumannii during infection.
    MeSH terms: Acinetobacter Infections/microbiology; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Larva/microbiology; Moths/microbiology; RNA Processing, Post-Transcriptional*; RNA, Bacterial/genetics; Virulence/genetics; Gene Expression Regulation, Bacterial; Transcriptome*
  2. Turkyilmaz I, Abdullah JY, Wilkins GN, Bernardi S, Varvara G
    Minerva Dent Oral Sci, 2024 Nov 20.
    PMID: 39565339 DOI: 10.23736/S2724-6329.24.04898-8
    BACKGROUND: The purpose of this study was to determine the accuracy of an intraoral scanner to digitally duplicate complete dentures and analyze the effects of mesh reduction of digital files on the surface area and volume accuracy of complete dentures, in vitro.

    METHODS: A mandibular complete denture was scanned by a desktop scanner to create a digital STL reference file (control). Fifteen identical scans were created by using an intraoral scanner and exported as STL files (test group). These 15 files were saved at 100% of the original scan resolution then reduced to 75%, 50%, 25%, and 10% of their original quality. These 75 scans were statistically analyzed by calculating The Hausdorff Distance (HD) and Dice Similarity Coefficients (DSC) to assess the variation between the mean reduced intraoral scanner files test and the control desktop scanner file and eventual inconsistencies. The volumes of the reduced mesh files were also compared with the 100% resolution intraoral mesh files to evaluate precision and trueness of the intraoral scanner.

    RESULTS: Reduced mesh files of 10%, 25%, 50%, 75% of the original scan yielded a percentage similarity average of 99.7%, indicating a very high precision value for the intraoral scanner. Also, the volumes of each associated mesh reduction slightly decreased with non-statistically significant results.

    CONCLUSIONS: This study concluded that the chosen intraoral scanner for this study provided very high trueness (98.34%) and precision (99.7%), and also the volumes of reduced mesh files slightly decreased but were not statistically significant.

  3. Jabri T, Daalah M, Alawfi BS, Gul J, Ahmed U, Shah MR, et al.
    Parasitol Res, 2024 Nov 20;123(11):387.
    PMID: 39565414 DOI: 10.1007/s00436-024-08389-6
    Acanthamoeba castellanii is the causative pathogen of a severe eye infection, known as Acanthamoeba keratitis and a life-threatening brain infection, named granulomatous amoebic encephalitis. Current treatments are problematic and costly and exhibit limited efficacy against Acanthamoeba parasite, especially the cyst stage. In parallel to drug discovery and drug repurposing efforts, drug modification is also an important approach to tackle infections, especially against neglected parasites such as free-living amoebae: Acanthamoeba. In this study, we determined whether modifying pentamidine and doxycycline through chitosan-functionalized graphene oxide loading enhances their anti-amoebic effects. Various concentrations of doxycycline, pentamidine, graphene oxide, chitosan-functionalized graphene oxide, and chitosan-functionalized graphene oxide loaded with doxycycline and pentamidine were investigated for amoebicidal effects against pathogenic A. castellanii belonging to the T4 genotype. Lactate dehydrogenase assays were performed to determine toxic effects of these various drugs and nanoconjugates against human cells. The findings revealed that chitosan-functionalized graphene oxide loaded with doxycycline demonstrated potent amoebicidal effects. Nanomaterials significantly (p 
    MeSH terms: Amebicides/pharmacology; Humans
  4. Asghar MA, Tang S, Wong LP, Yang P, Zhao Q
    J Ophthalmic Inflamm Infect, 2024 Nov 20;14(1):60.
    PMID: 39565496 DOI: 10.1186/s12348-024-00444-8
    BACKGROUND: Infectious uveitis is a significant cause of visual impairment worldwide, caused by diverse pathogens such as viruses, bacteria, fungi, and parasites. Understanding its prevalence, etiology, pathogenesis, molecular mechanism, and clinical manifestations is essential for effective diagnosis and management.

    METHODS: A systematic literature search was conducted using PubMed, Google Scholar, Web of Science, Scopus, and Embase, focusing on studies published in the last fifteen years from 2009 to 2023. Keywords included "uveitis," "infectious uveitis," "viral uveitis," and others. Rigorous inclusion and exclusion criteria were applied, and data were synthesized thematically. Gene symbols related to infectious uveitis were analyzed using protein-protein interaction (PPI) networks and pathway analyses to uncover molecular mechanisms associated with infectious uveitis.

    RESULTS: The search from different databases yielded 97 eligible studies. The review identified a significant rise in publications on infectious uveitis, particularly viral uveitis, over the past fifteen years. Infectious uveitis prevalence varies geographically, with high rates in developing regions due to systemic infections and limited diagnostic resources. Etiologies include viruses (39%), bacteria (17%), and other pathogens, substantially impacting adults aged 20-50 years. Pathogenesis involves complex interactions between infectious agents and the ocular immune response, with key roles for cytokines and chemokines. The PPI network highlighted IFNG, IL6, TNF, and CD4 as central nodes. Enriched pathways included cytokine-cytokine receptor interaction and JAK-STAT signaling. Clinical manifestations range from anterior to posterior uveitis, with systemic symptoms often accompanying ocular signs. Diagnostic strategies encompass clinical evaluation, laboratory tests, and imaging, while management involves targeted antimicrobial therapy and anti-inflammatory agents.

    CONCLUSION: This review underscores the complexity of infectious uveitis, driven by diverse pathogens and influenced by various geographical and systemic factors. Molecular insights from PPI networks and pathway analyses provide a deeper understanding of its pathogenesis. Effective management requires comprehensive diagnostic approaches and targeted therapeutic strategies.

  5. Loganathan T, Chan ZX, Hassan F, Kunpeuk W, Suphanchaimat R, Yi H, et al.
    PLoS One, 2024;19(11):e0314491.
    PMID: 39565764 DOI: 10.1371/journal.pone.0314491
    [This corrects the article DOI: 10.1371/journal.pone.0259546.].
  6. Abbasi SA, Rehman W, Rahim F, Hussain R, Hawsawi MB, Alluhaibi MS, et al.
    PMID: 39565952 DOI: 10.1515/znc-2024-0202
    Diabetes mellitus (DM) is a disorder which is raised at the alarming level and it is characterized by the hyperglycemia results from the impaired action of insulin, production of insulin or both of these simultaneously. Consequently, it causes problems or failure of different body organs such as kidneys, heart, eyes, nerve system. Since this disease cannot be completely cured until now, we aimed to design series of enzymes inhibitors and tested them for DM treatment. In this series, benzimidazole-based thiazolidinone bearing chalcone derivatives completed in a four step reaction and their structures were confirmed through various spectroscopic techniques. A significant efficacy on antidiabetic enzymes was observed, with IC50 values ranging from 25.05 ± 0.04 to 56.08 ± 0.07 μM for α-amylase and 22.07 ± 0.02 to 53.06 ± 0.07 μM for α-glucosidase. The obtained results were compared to those of the standard glimepiride drug (IC50 = 18.05 ± 0.07 µM for α-amylase and IC50 = 15.02 ± 0 .03 µM for α-glucosidase). The synthesized compounds showed promising antidiabetic potency. Moreover, a molecular docking study was conducted on the most active analogs of the compounds to better understand their interactions with the active sites of the targeted enzymes.
  7. Chong WN, Adiana G, Baharom IN, Kamaruzzaman BY, Takarina ND, Ong MC
    Mar Pollut Bull, 2024 Dec;209(Pt B):117281.
    PMID: 39566141 DOI: 10.1016/j.marpolbul.2024.117281
    Heavy metal pollution in aquatic ecosystems can lead to harmful accumulation in organisms like stingrays, posing potential health risks to humans. To assess this risk, 45 stingrays were sampled from Johor Waters (Pontian, Muar, and Batu Pahat) and analyzed for heavy metal content using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The average concentrations of Cu, Zn, As, Cd, Pb, and Hg in the fillets were 0.982±0.637, 31.3±5.93, 93.57±24.5, 0.039±0.047, 0.158±0.036, and 0.592±0.424 μg/g dw, respectively. Arsenic (As) was found at the highest concentration. The study indicates that fish size does not directly influence heavy metal accumulation and that metal levels vary by location. Calculated consumption limits for different metals are crucial for human safety. This data is valuable for evaluating contamination risks and guiding future research to protect marine ecosystems in Johor Waters.
    MeSH terms: Animals; Environmental Monitoring*; Malaysia; Skates (Fish)*
  8. Niu Q, Li D, Zhang J, Piao Z, Xu B, Xi Y, et al.
    Ageing Res Rev, 2024 Dec;102:102593.
    PMID: 39566741 DOI: 10.1016/j.arr.2024.102593
    Alzheimer's disease (AD), commonly known as senile dementia, is a neurodegenerative disease with insidious onset and gradually worsening course. The brain is particularly sensitive to senescence, and neuronal senescence is an important risk factor for the occurrence of AD. However, the exact pathogenesis between neuronal senescence and AD has not been fully elucidated so far. Neuronal senescence is characterized by the permanent stagnation of the cell cycle, and the changes in its structure, function, and microenvironment are closely related to the pathogenesis and progression of AD. In recent years, studies such as the Aβ cascade hypothesis and Tau protein phosphorylation have provided new strategies for the therapy of AD, but due to the complexity of the etiology of AD, there are still no effective treatment measures. This article aims to deeply analyze the pathogenesis between AD and neuronal senescence, and sort out various existing therapeutic methods, to provide new ideas and references for the clinical treatment of AD.
    MeSH terms: Aging/pathology; Aging/physiology; Animals; Brain/metabolism; Brain/pathology; Humans
  9. Saleh RO, Hamad HA, Najim MA, Menon SV, Kaur M, Sivaprasad GV, et al.
    Cell Biochem Biophys, 2024 Nov 20.
    PMID: 39567423 DOI: 10.1007/s12013-024-01617-x
    Exosomes are extracellular vesicles with a diameter ranging from 40 to 160 nm. They are produced by hepatocytes, cholangiocytes, hepatic stellate cells (HSCs), liver sinusoidal endothelial cells (LSECs) and Kupffer cells in liver tissue. The secretion of exosomes might vary in quantity and composition in reaction to multiple triggers and various stages of disease. They transport various payloads, such as proteins, DNAs, and RNAs, and enable cell interaction to regulate myriad physiological and pathological processes in liver tissue. Long non-coding RNAs (lncRNAs) are a crucial component of exosomes with an excellent capability to regulate multiple cellular activities such as differentiation, development, metabolism, proliferation, apoptosis, and activation. With the advancements in transcriptomic and genomic study methods and database management technology, the functions and mechanisms of exosomal lncRNAs in liver diseases have been well-studied. This article delves into the detailed role of exosomal lncRNAs in liver disease onset and progression, ranging from hepatocellular carcinoma (HCC) to liver fibrosis drug-induced liver damage (DILI) and steatotic liver diseases.
  10. Zhang J, Noor ZZ, Baharuddin NH, Setu SA, Hamzah MAAM, Zakaria ZA
    World J Microbiol Biotechnol, 2024 Nov 21;40(12):387.
    PMID: 39567441 DOI: 10.1007/s11274-024-04194-6
    This study highlights the biosorption capacity for Cd (II), Cu (II) and Pb (II) by a locally isolated Pseudomonas aeruginosa DR7. At initial concentrations of 150 mg L-1 and 240 min of contact time, P. aeruginosa DR7 showed a 62.56 mg/g removal capacity for Cd (II) at an optimum pH of 6.0, 72.49 mg/g for Cu (II) at an optimum pH of 6.0, and 94.2 mg/g for Pb (II) at an optimum pH of 7.0. The experimental data of Cd (II), Cu (II), and Pb (II) adsorbed by the pseudo-second-order kinetic model correlates well with P. aeruginosa DR7, with R2 all above 0.99, showing that the fitting effect was satisfactory. The isothermal adsorption processes of Cd (II) (0.980) and Cu (II) (0.986) were more consistent with the Freundlich model, whereas Pb (II) was more consistent with the Langmuir model (0.978). FTIR analysis suggested the involvement of hydroxyl, carbonyl, carboxyl, and amine groups present in the inner regions of P. aeruginosa cells during the biosorption process. SEM-EDS analysis revealed that after contact with metals, there were slight changes in the surface appearance of the cells, which confirmed the deposition of metals on the bacterial surface. There was also the possibility of the metals being translocated into the bacterial inner regions by the appearance of electron-dense particles, as observed using TEM. As a conclusion, the removal of metals from solutions using P. aeruginosa DR7 was a plausible alternative as a safe, cheap, and easily used biosorbent.
    MeSH terms: Adsorption; Biodegradation, Environmental*; Hydrogen-Ion Concentration; Kinetics; Soil Microbiology*; Spectroscopy, Fourier Transform Infrared
  11. Karthika A, Sudhakar C, Karuppasamy P, Tamilselvi B, Meena S, Anantharaju KS, et al.
    Sci Rep, 2024 Nov 20;14(1):28714.
    PMID: 39567594 DOI: 10.1038/s41598-024-80146-2
    A highly selective, sensitive caffeic acid (CA) detection based on calcium oxide nanoparticles (CaO NPs) derived from extract of Moringa oleifera leaves decorated graphitic carbon nitride covalently grafted poly vinyl alcohol (CaO/g-C3N4/PVA) nanocomposite modified glassy carbon electrode (GCE) was studied. A facile sonochemical method was adapted to synthesis nanomaterials and characterized by HR-TEM (High resolution transmission electron microscopy), FT-IR (Fourier transform infrared spectroscopy), XRD (X-ray diffraction), FE-SEM (Field emission scanning electron microscopy), EDX (Energy dispersive X-ray analysis), Mapping and BET (Brunauer-Emmett-Teller) analysis, and electrochemical techniques. The nanocomposite modified GCE exhibited an excellent catalytic performance to the oxidation of CA under optimized conditions owing to better electron transfer efficiency, conductivity and high surface area of the electrode material. The present electrochemical sensor showed high selectivity towards the determination of 10 µM CA in the presence of 100-fold higher concentrations of interferents. The modified CA sensor exhibited a wide sensing linear range from 0.01 µM to 70 µM and the detection limit (LOD) was found to be 0.0024 µM (S/N = 3) in 0.1 M phosphate buffer saline (PBS) as a supporting electrolyte at pH 7.0. The fabricated CA sensor provides an excellent stability, reproducibility and selectivity for the determination of CA. The modified CA sensor was applied to real blood plasma samples and obtained good recovery (97.6-100.1%) results.
  12. Shahrul AI, Pauzzi ER, Abas NDA, Yahya NA, Khan KAM, Nor MM
    BMC Public Health, 2024 Nov 20;24(1):3224.
    PMID: 39567923 DOI: 10.1186/s12889-024-20460-6
    BACKGROUND: The increasing number of young adults seeking cheaper and easily accessible orthodontic treatment from unlicensed practitioners in Malaysia poses significant risks to patients. Therefore, it is essential to evaluate their motivations and awareness regarding such practices. The objective of our study was to assess the knowledge, awareness, and perceptions of non-dentists offering orthodontic treatment among the Malaysian young adult population.

    METHODS: This was a cross-sectional study. An online self-administered questionnaire was distributed to Malaysian citizens aged 18-37 years. The questionnaire consisted of 11 questions that investigated their awareness of non-dentists offering orthodontic treatment, the harmful effects of braces fitted by non-dentists, and potential strategies to mitigate this phenomenon.

    RESULTS: The study was completed by 426 participants, predominantly Malay, with a mean age of 22.9 years. A total of 76.1% reported awareness of braces fixed by non-dentists, primarily through social media platforms such as Instagram and Facebook. Lower cost emerged as the predominant motive (83.6%) for opting for non-dentist orthodontic treatment, followed by no waiting list (48.8%). Notably, the majority of participants acknowledged the illegality (70%) and potential harm (77%) associated with non-dentists providing orthodontic treatment. Legal enforcement (53.1%) was identified as the preferred method for mitigating this practice. Occupation significantly influenced knowledge of illegal orthodontic treatment (p  0.05).

    CONCLUSION: The survey revealed that young adults are aware of and informed about non-dentists offering orthodontic treatment. While they identified cost as the primary reason for seeking such services, they also recognized legislation and public awareness through campaigns and social media as effective strategies to address this issue. Additionally, significant differences in legal awareness were observed among different occupational levels.

    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Orthodontics, Corrective/psychology; Surveys and Questionnaires; Young Adult
  13. Maspero J, De Paula Motta Rubini N, Zhang J, Sanclemente G, Amador JR, El Sayed MH, et al.
    World Allergy Organ J, 2024 Nov;17(11):100966.
    PMID: 39568731 DOI: 10.1016/j.waojou.2024.100966
    [This corrects the article DOI: 10.1016/j.waojou.2022.100724.].
  14. Farwa U, Sandhu ZA, Kiran A, Raza MA, Ashraf S, Gulzarab H, et al.
    RSC Adv, 2024 Nov 19;14(50):37164-37195.
    PMID: 39569125 DOI: 10.1039/d4ra05642f
    The global problem of ecological safety and public health necessitates, the development of new sustainable ideas for pollution remediation. In recent development, metal-organic frameworks (MOF) are the emerging technology with remarkable potential, which have been employed in environmental remediation. MOFs are networks that are created by the coordination of metals or polyanions with ligands and contain organic components that can be customized. The interesting features of MOFs are a large surface area, tuneable porosity, functional diversity, and high predictability of pollutant adsorption, catalysis, and degradation. It is a solid material that occupies a unique position in the war against environmental pollutants. One of the main benefits of MOFs is that they exhibit selective adsorption of a wide range of pollutants, including heavy metals, organics, greenhouse gases, water and soil. Only particles with the right combination of pore size and chemical composition will achieve this selectivity, derived from the high level of specificity. Besides, they possess high catalytic ability for the removal of pollutants by means of different methods such as photocatalysis, Fenton-like reactions, and oxidative degradation. By generating mobile active sites within the framework of MOFs, we can not only ensure high affinity for pollutants but also effective transformation of toxic chemicals into less harmful or even inert end products. However, the long-term stability of MOFs is becoming more important as eco-friendly parts are replaced with those that can be used repeatedly, and systems based on MOFs that can remove pollutants in more than one way are fabricated. MOFs can reduce waste production, energy consumption as compared to the other removal process. With its endless capacities, MOF technology brings a solution to the environmental cleansing problem, working as a flexible problem solver from one field to another. The investigation of MOF synthesis and principles will allow researchers to fully understand the potential of MOFs in environmental problem solving, making the world a better place for all of us.
  15. Zhang B, Liu L, Meng D, Kue CS
    Acta Radiol, 2024 Dec;65(12):1548-1559.
    PMID: 39569554 DOI: 10.1177/02841851241291931
    BACKGROUND: Cervical cancer is a major cause of morbidity and mortality among gynecological malignancies. Diagnostic imaging of lymph node (LN) metastasis for prognosis and staging is used; however, the accuracy in classifying the stage needs to improve.

    PURPOSE: To examine the accuracy of AI-based radiomics in diagnosis, prognosis assessment and predicting the diagnostic value of radiomics for pelvic LN metastasis in cervical cancer patients.

    MATERIAL AND METHODS: The study included 118 female patients with 660 LNs and 118 merged LNs. Four imaging histology models-decision tree, random forest, logistic regression, and support vector machine (SVM)-were created in this study. The imaging histology features were extracted from both the independent and merged LN groups. The AUC values for the test sets and the training sets of the four imaging histology models were compared for the independent LN group and the merged LN group. The DeLong test was used to compare the models.

    RESULT: The imaging histology prediction model developed in the merged LN group outperformed the independent LN group in terms of test set AUC (0.668 vs. 0.535 for decision tree, 0.841 vs. 0.627 for logistic regression, 0.785 vs. 0.637 for random forest, 0.85 vs. 0.648 for SVM) and accuracy (0.754 vs. 0.676 for decision tree, 0.780 vs. 0.671 for random forest, 0.848 vs. 0.685 for logistic regression, 0.822 vs. 0.657 for SVM).

    CONCLUSION: The constructed SVM imaging histology model for the merged LN group might be advantageous in predicting pelvic LN metastasis in cervical cancer.

    MeSH terms: Adult; Aged; Decision Trees; Female; Humans; Middle Aged; Neoplasm Staging*; Retrospective Studies; Support Vector Machine
  16. Wong KYR, Hakim I, Sawali H, Lim RCA, Mohd Mohsin NK
    Medeni Med J, 2024 Jun 28;39(2):132-135.
    PMID: 38940503 DOI: 10.4274/MMJ.galenos.2024.37729
    Tumors occurring in the submandibular space are infrequent among pediatric patients, and benign peripheral nerve tumors in this region are exceptionally rare. This study describes the uncommon occurrence of a schwannoma in the submandibular space in a child and describes its management. A 7-year-old child presented with a gradually enlarging swelling over a 7-month period in the submandibular region, clinically resembling a salivary gland tumor. There were no associated marginal mandibular, lingual, or hypoglossal nerve palsy. The mass was excised completely, and histopathological examination revealed it to be a schwannoma. It is appropriate to consider benign peripheral nerve tumors, such as schwannoma, in the differential diagnosis of submandibular space tumors in children.
  17. Elmahrouk A, Daoulah A, Panduranga P, Rajan R, Jamjoom A, Kanbr O, et al.
    Int J Surg, 2024 Nov 01;110(11):7142-7149.
    PMID: 39116452 DOI: 10.1097/JS9.0000000000002032
    BACKGROUND: Risk stratification for patients undergoing coronary artery bypass surgery (CABG) for left main coronary artery (LMCA) disease is essential for informed decision-making. This study explored the potential of machine learning (ML) methods to identify key risk factors associated with mortality in this patient group.

    METHODS: This retrospective cohort study was conducted on 866 patients from the Gulf Left Main Registry who presented between 2015 and 2019. The study outcome was hospital all-cause mortality. Various machine learning models [logistic regression, random forest (RF), k-nearest neighbor, support vector machine, naïve Bayes, multilayer perception, boosting] were used to predict mortality, and their performance was measured using accuracy, precision, recall, F1 score, and area under the receiver operator characteristic curve (AUC).

    RESULTS: Nonsurvivors had significantly greater EuroSCORE II values (1.84 (10.08-3.67) vs. 4.75 (2.54-9.53) %, P <0.001 for survivors and nonsurvivors, respectively). The EuroSCORE II score significantly predicted hospital mortality (OR: 1.13 (95% CI: 1.09-1.18), P <0.001), with an AUC of 0.736. RF achieved the best ML performance (accuracy=98, precision=100, recall=97, and F1 score=98). Explainable artificial intelligence using SHAP demonstrated the most important features as follows: preoperative lactate level, emergency surgery, chronic kidney disease (CKD), NSTEMI, nonsmoking status, and sex. QLattice identified lactate and CKD as the most important factors for predicting hospital mortality this patient group.

    CONCLUSION: This study demonstrates the potential of ML, particularly the Random Forest, to accurately predict hospital mortality in patients undergoing CABG for LMCA disease and its superiority over traditional methods. The key risk factors identified, including preoperative lactate levels, emergency surgery, chronic kidney disease, NSTEMI, nonsmoking status, and sex, provide valuable insights for risk stratification and informed decision-making in this high-risk patient population. Additionally, incorporating newly identified risk factors into future risk-scoring systems can further improve mortality prediction accuracy.

    MeSH terms: Machine Learning*; Aged; Female; Humans; Male; Middle Aged; Postoperative Complications/mortality; Postoperative Complications/epidemiology; Retrospective Studies; Risk Factors; Hospital Mortality; Risk Assessment/methods
  18. Xu XJ, Myint PK, Lee SWH, Ramasamy K, Lim SM, Majeed ABA, et al.
    Int J Clin Pharm, 2024 Dec;46(6):1453-1463.
    PMID: 39264491 DOI: 10.1007/s11096-024-01791-1
    BACKGROUND: While the effects of anticholinergic drug use have been increasingly highlighted, trends in anticholinergic use remain poorly understood.

    AIM: To determine the changes in frequency and pattern of anticholinergic drug use within a low- and middle-income country.

    METHOD: Comparisons were made between population-based datasets collected from Malaysian residents aged 55 years and older in 2013-15 and 2020-22. Anticholinergic exposure was determined using the anticholinergic cognitive burden (ACB) tool. Drugs with ACB were categorised according to the Anatomical Therapeutic Chemical (ATC) classification.

    RESULTS: A total number of 5707 medications were recorded from the 1616 participants included in the 2013-15 dataset. A total number of 6175 medications were recorded from 2733 participants in 2020-22. Two hundred and ninety-three (18.1%) and 280 (10.2%) participants consumed ≥ 1 medication with ACB ≥ 1 in 2013-15 and 2020-22 respectively. The use of nervous system drugs with ACB had increased (27 (0.47%) versus 39 (0.63%). The use of ACB drugs in the cardiovascular (224 (3.9%) versus 215 (3.4%)) and alimentary tract and metabolism (30 (0.52%) versus 4 (0.06%)) classes had reduced over time. Participants in 2020-22 were significantly less likely than those in 2013-15 to have total ACB = 1 - 2 (odds ratio [95% confidence interval] = 0.473[0.385-0.581]) and ACB ≥ 3 (0.251[0.137 - 0.460]) compared to ACB = 0 after adjustment for potential confounders (p 

    MeSH terms: Aged; Aged, 80 and over; Developing Countries; Drug Utilization/trends; Drug Utilization/statistics & numerical data; Female; Humans; Longitudinal Studies; Malaysia/epidemiology; Male; Middle Aged
  19. Teo HL, Abdul Wahab R, Zainal-Abidin MH, Mark-Lee WF, Susanti E
    Int J Biol Macromol, 2024 Nov;280(Pt 2):135787.
    PMID: 39304051 DOI: 10.1016/j.ijbiomac.2024.135787
    This study explores an eco-friendly delignification technique for raw oil palm leaves (OPL), highlighting the optimized conditions of choline chloride-lactic acid deep eutectic solvent (DES)-mediated ball milling pretreatment to maximize the co-production yields of highly crystalline cellulose and lignin. Our five-level-four-factor Taguchi design identified the optimal reaction settings for cellulose production (85.83 % yield, 47.28 % crystallinity) as 90-minute milling, 1500 rpm, mill-ball size ratio of 30:10, ball-to-sample mass ratio of 20:1, DES-to-sample mass ratio of 3:1. Conversely, the maximal lignin extraction yield (35.23 %) occurred optimally at 120-minute milling, 600 rpm, mill-ball size ratio of 25:5, ball-to-sample mass ratio of 20:1 and DES-to-sample mass ratio of 9:1. Statistical results showed that milling frequency (p-value ≤ 0.0001) was highly significant in improving cellulose crystallinity and yield, while DES-to-sample mass ratio (p-value ≤ 0.0001) was the most impacting on lignin yield. The thermogravimetric method affirmed the elevated cellulose thermal stability, corroborating the enhanced cellulose content (40.14 % to 73.67 %) alongside elevated crystallinity and crystallite size (3.31 to 4.72 nm) shown by X-ray diffractograms. The increased surface roughness seen in micrographs mirrored the above-said post-treatment changes. In short, our optimized one-pot dual-action pretreatment effectively delignified the raw OPL to produce cellulose-rich material with enhanced crystallinity and lignin solidity.
    MeSH terms: Solvents/chemistry; Arecaceae/chemistry
  20. Nordin AH, Yusoff AH, Husna SMN, Noor SFM, Norfarhana AS, Paiman SH, et al.
    Int J Biol Macromol, 2024 Nov;280(Pt 2):135799.
    PMID: 39307484 DOI: 10.1016/j.ijbiomac.2024.135799
    The long-term presence of pharmaceutical pollution in water bodies has raised public awareness. Nanocellulose is often used in adsorption to remove pollutants from wastewater since it is an abundant, green and sustainable material. This paper offers an extensive overview of the recent works reporting the potential of nanocellulose-based adsorbents to treat pharmaceutical wastewater. This study distinguishes itself by not only summarizing recent research findings but also critically integrating discussions on the improvements in nanocellulose production and sorts of alterations based on the type of pharmaceutical contaminants. Commonly, charged, or hydrophobic characteristics are introduced onto nanocellulose surfaces to accelerate and enhance the removal of pharmaceutical compounds. Although adsorbents based on nanocellulose have considerable potential, several significant challenges impede their practical application, particularly concerning cost and scalability. Large-scale synthesis of nanocellulose is technically challenging and expensive, which prevents its widespread use in wastewater treatment plants. Continued innovation in this area could lead to breakthroughs in the practical application of nanocellulose as a superior adsorbent. The prospects of utilization of nanocellulose are explained, providing a sustainable way to address the existing restriction and maximize the application of the modified nanocellulose in the field of pharmaceutical pollutants removal.
    MeSH terms: Adsorption; Pharmaceutical Preparations/isolation & purification; Pharmaceutical Preparations/chemistry; Nanostructures/chemistry
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