Browse publications by year: 2024

  1. Alnawafleh TM, Radzi Y, Alshipli M, Oglat AA, Alflahat A
    Curr Med Imaging, 2024;20(1):e15734056309829.
    PMID: 39492762 DOI: 10.2174/0115734056309829240909095801
    The most common primary malignant brain tumor is glioblastoma. Glioblastoma Multiforme (GBM) diagnosis is difficult. However, image segmentation and registration methods may simplify and automate Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) scan analysis. Medical practitioners and researchers can better identify and characterize glioblastoma tumors using this technology. Many segmentation and registration approaches have been proposed recently. Note that these approaches are not fully compiled. This review efficiently and critically evaluates the state-of-the-art segmentation and registration techniques for MRI and CT GBM images, providing researchers, medical professionals, and students with a wealth of knowledge to advance GBM imaging and inform decision-making. GBM's origins and development have been examined, along with medical imaging methods used to diagnose tumors. Image segmentation and registration were examined, showing their importance in this difficult task. Frequently encountered glioblastoma segmentation and registration issues were examined. Based on these theoretical foundations, recent image segmentation and registration advances were critically analyzed. Additionally, evaluation measures for analytical efforts were thoroughly reviewed.
    MeSH terms: Humans; Image Processing, Computer-Assisted/methods
  2. Shuvho MBA, Juri AZ, Basak AK, Kotousov A, Yin L
    J Mech Behav Biomed Mater, 2024 Nov 01;161:106794.
    PMID: 39504784 DOI: 10.1016/j.jmbbm.2024.106794
    While titanium alloy (Ti-6Al-4V) made by laser powder bed fusion (L-PBF) exhibits complex deformation behaviors, its important micromechanical properties in relation to loading directions are not fully understood. This research aims to investigate the micromechanical behaviors of printed L-PBF Ti-6Al-4V alloys under vertical (i.e., the loading direction perpendicular to printed layers) and horizontal (i.e., the loading direction parallel to printed layers) compressions using in-situ scanning electron microscopy (SEM) micropillar techniques. Ti-6Al-4V alloys were L-PBF-printed using a 45° rotate scanning strategy with vertical and horizontal build directions. The microstructures of the two alloys were analyzed using the SEM with energy-dispersive X-ray spectroscopy (EDS). The titanium alloy micropillars were produced using focused ion beam (FIB) milling in the SEM. In-situ SEM micropillar compressions were conducted using a flat diamond indenter. Vertical alloy had smaller cross-patterned finer α' martensite than horizontal one. While both vertical and horizontal micropillars showed elastic-plastic behaviors, the former had significantly higher yield, fracture, and compression strength values, as well as resilience and toughness, than the latter, leading to the formation of favorable shear bands. Both micropillars exhibited ductile fractures but had distinct failure mechanisms. The ductile fracture in the vertical micropillars was due to strain hardening, large plastic deformation, and shear band formation, while the ductile fracture in the horizontal ones was attributed to compression-induced bending and plastic buckling. The micromechanical characteristics of L-PBF Ti-6Al-4V materials provides an important insight into the small-scale deformation and failure mechanisms of the alloys influenced by loading directions.
  3. Zhang Y, Yusof FM
    Acta Psychol (Amst), 2024 Nov;251:104590.
    PMID: 39504842 DOI: 10.1016/j.actpsy.2024.104590
    Drawing on Bronfenbrenner's ecological system, the present study employs a qualitative longitudinal approach to delve into the emotional experiences and ecological factors that contribute to these emotions in eight novice EFL teachers from four Chinese public universities. Based on data collected from semi-structured interviews and case documents, this study identifies four types of emotional experiences-fear, anger, sadness, and joy-in their first school year. The findings revealed that their emotional experiences were shaped by college students, teachers' teaching competence, EFL teaching-related activities in the microsystem, interpersonal relationships in the mesosystem, novice teacher education and development systems in the exosystem, traditional cultural values and English curriculum reforms in the macrosystem, and changes in the emotional experiences over time in the chronosystem. The implications for novice EFL teachers, teacher educators, school leaders, and policymakers are proposed.
    MeSH terms: Adult; China; Female; Humans; Interpersonal Relations; Longitudinal Studies; Male; Students/psychology; Universities; Qualitative Research; Young Adult; School Teachers
  4. Lee JC, Urakami S, Hinou H
    Int J Biol Macromol, 2024 Dec;282(Pt 5):137178.
    PMID: 39505162 DOI: 10.1016/j.ijbiomac.2024.137178
    Escherichia coli O111 is a critical pathogenic E. coli serotype that causes severe, potentially fatal complications. Despite its reported variation, only one structure of the O-antigen polysaccharide from E. coli O111 has been reported. Here, a substructure of the O-antigen from E. coli O111 was characterized using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and NMR analysis. MALDI glycotyping revealed differing O-antigen repeating unit masses of Δm/z 787 and 828 in the E. coli strains and lipopolysaccharides from the O111 serogroup. This variation was caused by the replacement of the hexose residue with hexosamine in the repeating units, which was further confirmed by LIFT-TOF/TOF analysis. Structural elucidation of the O111 substructure by NMR analysis further demonstrated replacement of the hydroxyl group with an N-acetyl group on the terminal glucose residue of the O-antigen pentasaccharide repeating unit. To our knowledge, this study is the first to provide a detailed structural analysis of a new O-antigen substructure from the E. coli O111 serogroup.
    MeSH terms: Carbohydrate Sequence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization*; Serogroup
  5. Yusuf J, Firdaus AHM, Sapuan SM, Rashid U, Ilyas RA, Hassan MR, et al.
    Int J Biol Macromol, 2024 Dec;282(Pt 5):137244.
    PMID: 39505165 DOI: 10.1016/j.ijbiomac.2024.137244
    Nanocellulose-graphene hybrid composites for high-performance uses have been the focus of recent research. In contrast to graphene, which has great conductivity and mechanical strength, nanocellulose possesses special qualities like renewability and biocompatibility but lacks electrical conductivity. Since graphene-nanocellulose has such promising features, efforts to make flexible electronic composites employing them have accelerated. However, the environmental impacts are needed to be addressed prior to the applications of these hybrid composites. This review article explores environmental aspects for nanocellulose-graphene hybrid composites because of their sustainability, which is a major step in the right direction. The article also emphasizes how these composites have the potential to transform several industries and open the door to a more environmentally friendly future. This paper explores into the most recent developments in nanocellulose-graphene hybrid composites, highlighting its environmental benefits and adaptability. These composites offer remarkable performance by combining the strength and conductivity of graphene with the mechanical, electrical, and thermal capabilities of nanocellulose.
    MeSH terms: Electric Conductivity; Environment; Nanostructures/chemistry; Nanocomposites/chemistry
  6. Ang ZY, Kong YL, Nesran ZNM, Lee SWH
    BJU Int, 2024 Nov 06.
    PMID: 39505722 DOI: 10.1111/bju.16581
  7. Ali ASB, Al Allam AM, Ali SIA, Isleem HF, Babalghaith AM, Shaffie E, et al.
    Sci Rep, 2024 Nov 06;14(1):26873.
    PMID: 39505958 DOI: 10.1038/s41598-024-78032-y
    This study investigated the chemical properties of peat microparticles modified asphalt (Pt.M.A.). The originality of the study resides in the examination of the chemical characteristics of peat microparticles (Pt.) modified asphalt (Pt. M.A.) utilising FTIR, SEM, SFE, and XRD methodologies. This encompasses Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), surface free energy (SFE), and X-ray diffraction (XRD). Initially, FTIR examined the functional groups of both unaltered and altered asphalt binders. The SEM images reveal improved compatibility, showcasing superior diffusion of the modifier across the asphalt. A further critical factor is that improved adhesion properties, according to the SFE study, indicate that modified binders generally offer more SFE compared to unmodified binders. The XRD measurements revealed a semi-crystalline structure in the Pt. modifier and an amorphous structure in the basal asphalt binder. The integration of Pt. into the asphalt cement resulted in modifications to the phases of both constituents, culminating in the emergence of a new semi-crystalline phase inside the modified asphalt binder. These data suggest that peat microparticles (Pt.) can improve the efficacy of asphalt binders by enhancing compatibility, adhesion, and resistance to ageing.
  8. Nordin FZ, Shaharir SS, Mohamed Said MS, Mohd R, Sakthiswary R, Tengku Mohd TAM, et al.
    Lupus, 2024 Dec;33(14):1645-1653.
    PMID: 39506570 DOI: 10.1177/09612033241297548
    INTRODUCTION: Heterogeneity of the clinical manifestations of systemic lupus erythematosus (SLE) may lead to diagnostic delays. This study is aimed at determining the health-seeking behaviour patterns and factors associated with diagnostic delays in a multi-ethnic SLE cohort in Malaysia.

    METHODOLOGY: This was a cross-sectional study involving SLE patients who visited our institute between January 2020 and June 2021. A review of the medical records and face-to-face interviews were conducted to obtain sociodemographics, SLE disease characteristics and the intervals from the first symptoms to the diagnosis. Health-seeking behaviours were assessed by asking about the patients' first action during the initial symptoms and were divided into: (i) seeking professional health personnel; (ii) self-treatment; and (iii) the use of the internet as a primary source of information. Diagnostic delays were defined as the interval between initial symptoms and SLE diagnosis of more than 6 months. Low-level disease activity state (LLDAS) at 12 months was assessed from the medical records. Univariate and multivariate logistic regression analysis was subsequently conducted to determine factors associated with diagnostic delays.

    RESULTS: Among the 154 patients included in the study, 24% (n = 37) had delayed diagnosis. The delay was significantly higher among the Indian versus Malay versus Chinese (42.9% vs 28% vs 10.8%, p = 0.037). Patients with rash tend to have delayed diagnosis (37.8% vs 22.2%, p = 0.08) while fewer patients with frothy urine had delayed diagnosis (8.1% vs 21.4%, p = 0.09). No significant association was found between health-seeking behaviours and diagnostic delays. The rate of LLDAS at 12 months was significantly lower among patients with delayed diagnosis (43.2% vs 70.0%, p = 0.006). Chinese ethnicity remained the only significant factor associated with lesser diagnostic delays in the multivariate analysis, with OR 0.30 (CI 0.09-0.93), p = 0.037.

    CONCLUSION: There were ethnic disparities in the early diagnosis of SLE in Malaysia, with Indian patients having a longer interval between the first symptom and diagnosis while the Chinese were associated with lower diagnostic delays. Early diagnosis predicted early attainment of LLDAS, suggesting that prompt recognition of the initial SLE symptoms is important.

    MeSH terms: Adult; Cross-Sectional Studies; Ethnic Groups; Female; Humans; Malaysia; Male; Middle Aged; Cohort Studies; Health Behavior; Young Adult; Delayed Diagnosis*
  9. Azim T, Khan AH, Sadiq F, Sulaiman SAS, Khan A, Ain Q
    BMC Nephrol, 2024 Nov 06;25(1):399.
    PMID: 39506723 DOI: 10.1186/s12882-024-03821-6
    BACKGROUND: Recent reports have revealed that nephropathy leading to kidney injury (KI) is a prevalent complication of COVID-19 and is linked to high mortality and morbidity in diabetes mellitus type II (DM-T-II) patients. This systematic literature review and meta-analysis aimed to critically analyze existing studies and evidence on the impact of COVID-19 on nephropathy and kidney injury in diabetes mellitus type II (DM-T-II) patients.

    METHOD: A systematic search was conducted in the Web of Science (WoS), PubMed and Cochrane databases for relevant studies published between March 2020 and July 2023. To ensure the integrity of the systematic literature review and meta-analysis, observational studies that specifically reported post-COVID-19 kidney injury in DM-T2 patients were included, whereas we did not include articles in the press, meta-analyses, case reports, case series, Diabetes Type-I articles or non-English papers. The primary outcome was kidney injury in patients with type II diabetes after contracting COVID-19. The protocol for this study was published on PROSPERO (registration number CRD42023413887).

    RESULTS: Initially, 6,339 articles were included in the search, from which only 6 observational studies were selected by following the 2020 PRISMA statement. The quality of the evidence was assessed by a tool provided by the National Institutes of Health (observational studies). The total number of participants included in the studies was 14,723. Our systematic literature review and meta-analysis provide compelling evidence that kidney injury is a prevalent complication of COVID-19 infection in the type II diabetes population, with a pooled odds ratio of 2.27 (95% CI: 2.05-2.51; p 

    MeSH terms: Humans; Acute Kidney Injury/etiology; Acute Kidney Injury/epidemiology
  10. Mohanraj D, S D, Balachandran PK
    Heliyon, 2024 Oct 30;10(20):e39598.
    PMID: 39506935 DOI: 10.1016/j.heliyon.2024.e39598
    The robust rotor structure and fault-tolerance characteristics of the Switched Reluctance Motors (SRMs) are the best choice for next-generation Electric Vehicle (EV) applications. This machine has few restraints like high torque and flux ripples. However, the existing Model Predictive Control (MPC) using multiple control objectives and maximum sectors in the switching table results in high torque ripples due to the improper sector partition, Voltage Vectors (VVs) and weight factor (K 1 ) selection. This paper proposes a Finite Set-Model Predictive Control (FS-MPC) for an analytical model of a non-linearity SRM machine to analyze the torque ripple performance. The proposed VVs are derived using sector partition based on the rotor position. The control is designed as a single cost function with the weighting factor contributing to smooth torque by selecting optimal control signals. Simulation studies and experiments with a four-phase 8/6 SRM drive verifies the enhanced FS-MPC for real-time implementation. The dynamic speed and ripple values of SRM Drives are measured using a mixed signal oscilloscope and the sensor probes. The laboratory outcomes calculate the analytical equations to validate the findings. The calculated value of torque ripple is 9 % through this FS-MPC. The study reveals that the proposed method is well suited for torque ripple reduction than flux ripples.
  11. Jones SU, Kee BP, Chew CH, Yeo CC, Chua KH, Puah SM
    Heliyon, 2024 Oct 30;10(20):e39634.
    PMID: 39506957 DOI: 10.1016/j.heliyon.2024.e39634
    Bacterial small RNAs (sRNAs) play crucial roles in coordinating gene regulatory networks in various physiological processes, including biofilm formation. In this study, RNA sequencing was performed on biofilm (n = 4) and planktonic (n = 4) cells harvested at 10 h (pre-stationary phase of biofilm development) to identify biofilm-associated sRNAs in human methicillin-susceptible Staphylococcus aureus (MSSA) recovered from urine isolate. A total of 56 highly expressed sRNAs were identified with 15 overlapping sRNA genes (srn_9348, sprD, sRNA205, sRNA288, srn_2467, Sau-25, srn_2468, sRNA260, sRNA200, RsaE, sRNA397, Teg55, Teg60, RsaX05 and Teg140). Further validation through RT-qPCR analysis of nine sRNAs revealed that srn_9348 and sRNA260 were significantly expressed in the biofilm cells of urine sample. Both sRNAs were predicted to interact with mRNA genes including intracellular adhesin A (icaA) and host factor protein (hfq) involved in biofilm formation via cis-acting and trans-acting using CopraRNA analysis. Therefore, both sRNAs merit further investigations via reverse genetic approaches to elucidate their mechanism of translational regulation. In summary, the transcriptomic analysis conducted in this study offers new insights into the potential regulatory roles of sRNAs in MSSA biofilm development within the urinary environment.
  12. Fahmi MZ, Sugito SFA, Wibrianto A, Novania S, Widyastuti S, Ahmad MA, et al.
    Nanotheranostics, 2024;8(4):521-534.
    PMID: 39507106 DOI: 10.7150/ntno.96559
    This study introduces an innovative magnetic-based multifunctional anti-cancer drug carrier aiming to enhance the efficacy of curcumin in cancer therapy. The research investigates the potential of Graphene Quantum Dots (GQDs) as a curcumin drug delivery system for inhibiting in vivo cancer growth. GQDs with a particle diameter below 10 nm were synthesized via hydrothermal and Hummers methods, exhibiting homogeneity and crystalline structure according to AFM and XRD analyses. FTIR analysis confirmed functionalization success, revealing the formation of bonds between GQDs and curcumin. The optical properties of GQDs were assessed using a UV-Vis spectrophotometer and spectrofluorometer, resulting in vigorous fluorescence with a quantum yield of 1.32%. Subsequently, loading curcumin onto GQDs (CQDs/cur) resulted in an efficient system for delivering the anti-cancer drug, demonstrating significant in vivo efficacy. It was indicated by reduced tumor diameter and increased body weight in mice. Furthermore, the release kinetics of curcumin from GQDs were analyzed using the Peppas-Sahlin equation under varying pH conditions (4, 7, and 9), revealing the highest release rate in acidic conditions. In conclusion, this study highlights the potential of GQDs as highly efficient carriers for targeted curcumin delivery, showcasing promising prospects in cancer treatment.
    MeSH terms: Animals; Drug Carriers/chemistry; Humans; Kinetics; Neoplasms/drug therapy; Drug Delivery Systems/methods; Cell Line, Tumor; Mice; Drug Liberation
  13. McBenedict B, Hauwanga WN, Amadi ES, Abraham AA, Sivakumar R, Okere MO, et al.
    Cureus, 2024 Oct;16(10):e70993.
    PMID: 39507141 DOI: 10.7759/cureus.70993
    Hypertrophic cardiomyopathy (HCM) is a hereditary cardiovascular condition marked by heart muscle thickening, fibrosis, and myocardial disorders. It is often inherited in an autosomal dominant pattern. Symptoms include dyspnea, fatigue, palpitations, dizziness, syncope, and an increased risk of sudden cardiac death (SCD). Genetic studies have identified many asymptomatic carriers, indicating a higher prevalence of HCM. Advances in genetic testing (GT) and novel therapies, such as cardiac myosin inhibitors, have significantly impacted the diagnosis and management of HCM. This integrative review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and aimed to synthesize information regarding the impact of GT on the diagnosis and management of HCM patients. An electronic search was conducted on May 17, 2024, across PubMed, Embase, Scopus, Web of Science, and Cochrane databases, covering January 2020 to May 2024. Inclusion criteria were studies involving adult HCM patients who underwent GT and follow-up. Exclusion criteria included non-human studies, pediatric cases, non-HCM-related GT, non-peer-reviewed articles, systematic reviews, conference abstracts, and editorials. From 1,155 articles identified, 42 met the inclusion criteria after applying filters and removing duplicates. GT identified pathogenic variants in a significant proportion of HCM patients, enhancing diagnostic accuracy and management. Key mutations were found in myosin binding protein C3 and myosin heavy chain 7 genes. GT facilitated personalized management strategies, including risk stratification for SCD and family screening. Patients with identified mutations often required closer monitoring and tailored treatments. GT has revolutionized the diagnosis and management of HCM. The integration of genetic data has improved risk stratification, facilitated early intervention, and enhanced patient outcomes. Despite these advances, challenges remain in identifying genetic variants in some patients, emphasizing the need for continuous improvement in genetic panels and diagnostic methods. This review highlights the significant role of GT in optimizing HCM care through precise risk assessment and tailored treatment strategies.
  14. El Moshy S, Radwan IA, Matoug-Elwerfelli M, Abdou A, Abbass MMS
    Clin Cosmet Investig Dent, 2024;16:453-465.
    PMID: 39507288 DOI: 10.2147/CCIDE.S478045
    PURPOSE: This study aims to investigate the biomimetic effect of agarose hydrogel loaded with enamel matrix derivative (EMD-agarose) alone or in combination with nano-hydroxyapatite (n-HA-EMD-agarose) on the remineralization of human demineralized enamel.

    METHODS: Extracted human mandibular third molars were sectioned into 54 buccal and lingual halves. Acid-resistant nail varnish was applied to each half, except for two enamel windows. Enamel surface microhardness, energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM) analyses were conducted to evaluate enamel surfaces at baseline, following demineralization with 37% phosphoric acid, and after each hydrogel application and remineralization for two, four, and six days. Remineralization was performed using a phosphate solution at 37°C.

    RESULTS: At day 6 following remineralization, a statistically significant higher mean microhardness was recorded in n-HA-EMD-agarose hydrogel (260.87 ± 3.52) as compared to EMD-agarose hydrogel (244.63 ± 2.76) (p = 0.027). Similarly, n-HA-EMD-agarose hydrogel showed a higher mean calcium (46.31 ± 2.78), phosphorous (24.92 ± 0.826), and fluoride (0.909 ± 0.053) weight percentage compared to EMD-agarose hydrogel calcium (19.64 ± 1.092), phosphorous (19.64 ± 1.092), and fluoride (0.7033 ± 0.0624) weight percentage (p < 0.05). Further, SEM analysis revealed a substantial deposition of n-HA following the application of the n-HA-EMD-agarose hydrogel, whereas the EMD-agarose exhibited a relatively smooth enamel surface with less visible enamel rods due to mineral deposition.

    CONCLUSION: The combined n-HA-EMD-agarose hydrogel demonstrated improved surface microhardness of the remineralized enamel and enhanced mineral content deposition, indicating its potential as a biomimetic approach for dental enamel repair.

  15. Abdurabuh A, Hamid MD, Che Hassan CR, Fatani MI
    J Multidiscip Healthc, 2024;17:5021-5033.
    PMID: 39507375 DOI: 10.2147/JMDH.S480496
    BACKGROUND: The impact of hospital accreditation on the organizational safety culture among healthcare workers, an essential indicator of patient safety, has yet to be directly quantified in Saudi Arabia's healthcare system. This study aims to investigate this impact to sustain and maintain a positive safety culture in Saudi Arabia's healthcare institutions.

    METHODS: A cross-sectional assessment was conducted in five public hospitals in Makkah. Three hundred forty healthcare workers participated using a self-administered questionnaire. Data were analyzed using descriptive statistics, ANOVA, one-sample t-test, and multiple regression for a comprehensive understanding.

    RESULTS AND DISCUSSION: Regression analysis revealed significant gender differences in patient safety ratings (B = 0.480, p < 0.001). Age positively influenced scores, with higher ages resulting in higher scores (B = 0.127, p = 0.041). The ratings were also associated with respondents' nationality (B = 0.169, p < 0.001) and education levels (B = -0.186, p < 0.001). Respondents rated disasters and training as the highest in patient safety culture, followed by facility safety and security, hazards and hazardous materials safety, utility and building safety, fire safety, and quality improvement. At the same time, leadership, commitment, and support received the lowest score.

    CONCLUSION: This study illustrates a strong connection between accreditation and improved patient safety, emphasizing the importance of quality improvement and leadership commitment. These insights can guide policymakers and healthcare executives in Saudi Arabia and similar countries toward developing a robust patient safety culture. It stresses the importance of considering human factors and organizational culture when developing patient safety models.

  16. Ong YT, Sinnathamby A, Tan JH, Ravindran N, Lim SX, Hiew AWH, et al.
    PMID: 39508141 DOI: 10.1177/10499091241298281
    Background: The cost of caring for patients and their families in the midst of interconnected resource, ethical, moral, legal and practical considerations compromises a physician's emotional and physical well-being and therefore patient care. Whilst the cost of caring is historically best associated with compassion fatigue, data has suggested that this may extend to other related concepts, such as vicarious trauma, secondary traumatic stress and burnout. In particular, palliative care physicians are especially vulnerable as they witness and encounter more cases of death and dying. Methods: This study aims to provide a more clinically relevant notion of the cost of caring amongst palliative care physicians in Malaysia. 11 physicians underwent semi-structured interviews as part of the Systematic Evidence-Based Approach (SEBA) for prospective studies. Results: Analysis of the interview transcripts revealed the following domains: (1) conceiving the costs of caring; (2) risk factors; and (3) support mechanisms. Conclusion: This SEBA-guided study into the cost of caring amongst Malaysian palliative care physicians suggests that the costs of caring extend beyond encapsulating moral distress, compassion fatigue, vicarious trauma, secondary traumatic stress and burnout. Rather, the data suggests a personalized notion that varies with individual and contextual factors which are in flux and change over time. A longitudinal, personalized and holistic mentoring program is therefore proposed to counter this cost.
  17. Wardana AP, Kristanti AN, Aminah NS, Fahmi MZ, Raoov M, Indriani
    Int J Nanomedicine, 2024;19:11039-11053.
    PMID: 39502639 DOI: 10.2147/IJN.S474611
    BACKGROUND: After cardiovascular disease, cancer is one of the leading causes of death due to uncontrolled cell growth. Breast cancer is among the most prevalent types of cancer. Zingiber officinale Roscoe. rich in phenolic compounds, which can stimulate and function as endogenous antioxidants.

    PURPOSE: Investigation of the in vivo chemopreventive has the potential of nano Z. officinale Roscoe (Zo-NPs) in breast cancer.

    STUDY DESIGN: Using female Mus musculus Balb/c induced with benzo[α]pyrene, the chemopreventive action of Z. officinale Roscoe. nanoencapsulated using κ-carrageenan was assessed.

    RESULTS: Z. officinale Roscoe Extract. contains 58 compounds, with the main component being [6]-gingerol with [6]-gingerol content being 697.65 ± 8.52 mg/g extract. Nanoencapsulation of Z. officinale Roscoe. has been successfully prepared with a particle size of 483.30 ± 11.23 nm. Zo-NPs are generally resistant to pH, temperature, and salt content variations. Compared to group C1, which underwent ductular dilatation, the administration of Zo-NPs (group T2) to female Mus musculus Balb/c, induced by benzo[α]pyrene, revealed no histological alterations in breast tissue. Moreover, administering Zo-NPs can raise blood serum levels of CAT, GSH, and SOD. In addition, it showed a greater ability to lower TNF-α levels than the T1 group, which received Z. officinale Roscoe extract. (Zo).

    MeSH terms: Animals; Antioxidants/pharmacology; Antioxidants/chemistry; Benzo(a)pyrene; Breast Neoplasms/drug therapy; Breast Neoplasms/pathology; Breast Neoplasms/prevention & control; Carrageenan/pharmacology; Catechols/pharmacology; Catechols/chemistry; Fatty Alcohols/pharmacology; Fatty Alcohols/chemistry; Female; Mice, Inbred BALB C*; Particle Size; Tumor Necrosis Factor-alpha/blood; Tumor Necrosis Factor-alpha/metabolism; Chemoprevention/methods; Mice; Nanoparticles/administration & dosage; Nanoparticles/chemistry
  18. Balasubaramaniam D, Lim YW, Retnasabapathy S, A Qamarruddin F, Singh S
    Cutan Ocul Toxicol, 2024 Dec;43(4):390-395.
    PMID: 39498579 DOI: 10.1080/15569527.2024.2423265
    PURPOSE: To compare the thickness of the retinal nerve fibre layer (RNFL), ganglion cell layer (GCL), inner plexiform layer (IPL), and choroid in healthy electronic cigarette smokers and non-smokers using spectral domain optical coherence tomography (SD-OCT).

    MATERIAL AND METHOD: 25 healthy electronic cigarette smokers and 25 age- and gender-matched healthy non-smokers were included in the study. RNFL, GCL, IPL and choroidal thickness were measured by SD-OCT using an automated programme. After normality tests, an independent sample t-test was used to analyse the differences in RNFL, GCL, IPL, and choroidal thickness values between the groups.

    RESULTS: The mean age of electronic cigarette smokers and non-smokers was 33.68 and 33.64 years, respectively. The mean smoking history was 6.6 years (range 5-8 years). Most of the participants smoked 2-5 ml of e-liquid per day (52%), while 36% smoked more than 5 ml and 12% smoked less than 2 ml per day. The mean intraocular pressure in the electronic cigarette smoker group was 15.0 mmHg, while the non-smoker group was 15.32 mmHg. The mean axial length in the electronic cigarette smoker group and non-smoker group was 23.36 and 23.63 mm, respectively. No significant difference was observed regarding RNFL, GCL, IPL or choroidal thickness between both groups.

    CONCLUSION: The thickness of the RNFL, GCL, IPL, and choroid was found to be similar in both the healthy electronic cigarette smokers and non-smokers groups.

    MeSH terms: Adult; Female; Humans; Intraocular Pressure; Male; Middle Aged; Retina/pathology; Case-Control Studies; Tomography, Optical Coherence*; Young Adult; Electronic Cigarettes
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