Browse publications by year: 2024

  1. Brassington L, Arner AM, Watowich MM, Damstedt J, Ng KS, Lim YAL, et al.
    Evol Med Public Health, 2024;12(1):214-226.
    PMID: 39484023 DOI: 10.1093/emph/eoae014
    More than 60 years ago, James Neel proposed the Thrifty Genotype Hypothesis to explain the widespread prevalence of type 2 diabetes in Western, industrial contexts. This hypothesis posits that variants linked to conservative energy usage and increased fat deposition would have been favored throughout human evolution due to the advantages they could provide during periods of resource limitation. However, in industrial environments, these variants instead produce an increased risk of obesity, metabolic syndrome, type 2 diabetes, and related health issues. This hypothesis has been popular and impactful, with thousands of citations, many ongoing debates, and several spin-off theories in biomedicine, evolutionary biology, and anthropology. However, despite great attention, the applicability and utility of the Thrifty Genotype Hypothesis (TGH) to modern human health remains, in our opinion, unresolved. To move research in this area forward, we first discuss the original formulation of the TGH and its critiques. Second, we trace the TGH to updated hypotheses that are currently at the forefront of the evolutionary medicine literature-namely, the Evolutionary Mismatch Hypothesis. Third, we lay out empirical predictions for updated hypotheses and evaluate them against the current literature. Finally, we discuss study designs that could be fruitful for filling current knowledge gaps; here, we focus on partnerships with subsistence-level groups undergoing lifestyle transitions, and we present data from an ongoing study with the Orang Asli of Malaysia to illustrate this point. Overall, we hope this synthesis will guide new empirical research aimed at understanding how the human evolutionary past interacts with our modern environments to influence cardiometabolic health.
  2. Huda HS, Majid NBA, Chen Y, Adnan M, Ashraf SA, Roszko M, et al.
    Food Sci Nutr, 2024 Oct;12(10):6938-6955.
    PMID: 39479640 DOI: 10.1002/fsn3.4346
    Tea, a culturally significant beverage, originated around 2700 B.C. in ancient Chinese civilization, with a profound understanding of its therapeutic properties. Herbal medicines from diverse plant sources have been esteemed for their phytochemical content. Today, tea's appeal spans the globe, with various processing techniques creating distinct tea varieties. This review article comprehensively explores tea and herbal teas, encompassing their origins, types, trade history, health benefits, chemical composition, and market and future dynamics. This review examines tea's evolution from ancient China to its global significance and analyzes the impact of tea trade routes on cultural exchanges and trade dynamics. The review covers conventional teas (black, green, and oolong), blended teas, and herbal teas. It primarily focuses on herbal beverages' chemical composition and active components derived from diverse plants and botanicals, highlighting their traditional uses and health-promoting applications. The review provides valuable insights into the dynamic herbal tea market, growth, consumer preferences, industry trends, and future aspects of the herbal beverage. Additionally, it explores the proper classification and preparation of herbal drinks for maximum benefits, shedding light on tea manufacturing and preparation processes. This review is a valuable resource for tea enthusiasts, health-conscious individuals, and industry stakeholders, offering profound insights into teas and their multifaceted allure.
  3. Younas K, Afzaal M, Saeed F, Shankar A, Kumar Bishoyi A, Khare N, et al.
    J Sci Food Agric, 2024 Oct 30.
    PMID: 39474642 DOI: 10.1002/jsfa.13953
    Each year, approximately million tons of waste is generated from eggshells disposed of in landfills. This waste is challenging to manage because of the odor and microbiological development. However, eggshells have potential applications as a solid byproduct. They can be modified and used in various industries such as metal, polymer and ceramic composites, in the production of biodiesel, heavy metal absorption from wastewater, and even as a biomaterial to substitute bone tissues. Furthermore, eggshells can be used as a cheap adsorbent for the treatment of contaminated waterways. They are also a great source of calcium and fertilizer for plants, animals and humans. Chicken eggshells can even be used as a catalyst for converting waste frying oil into biofuel. This review highlights the challenges and opportunities of eggshell waste valorization in the food industry. © 2024 Society of Chemical Industry.
  4. Suhandi C, Wilar G, Narsa AC, Mohammed AFA, El-Rayyes A, Muchtaridi M, et al.
    Drug Des Devel Ther, 2024;18:4723-4748.
    PMID: 39469723 DOI: 10.2147/DDDT.S478388
    α-Mangostin, initially identified in 1855, is a xanthone derivative compound predominantly located in the pericarp of the mangosteen fruit (Garcinia mangostana L). This compound is known for its beneficial properties as an antioxidant and anti-inflammatory agent, still holding promise for potential benefits in other related pathologies. In the investigative process, computational studies have proven highly valuable in providing evidence and initial screening before progressing to preclinical and clinical studies. This review aims to present the pharmacological findings and mechanisms of action of α-mangostin based on computational studies. The compilation of this review is founded on the analysis of relevant articles obtained from PubMed, Scopus, and ScienceDirect databases. The study commences with an elucidation of the physicochemical characteristics, drug-likeness, pharmacokinetics, and toxicity profile of α-mangostin, which demonstrates that α-mangostin complies with the Lipinski's Rule of Five, exhibits favorable profiles of absorption, distribution, metabolism, and excretion, and presents low toxicity. Subsequent investigations have revealed that computational studies employing various software tools including ArgusLab, AutoDock, AutoDock Vina, Glide, HEX, and MOE, have been pivotal to comprehend the pharmacology of α-mangostin. Beyond its well established roles as an antioxidant and anti-inflammatory agent, α-mangostin is now recognized for its pharmacological effects in Alzheimer's disease, diabetes, cancer, chronic kidney disease, chronic periodontitis, infectious diseases, and rheumatoid arthritis. Moreover, α-mangostin is projected to have applications in pain management and as a potent mosquito larvicide. All of these findings are based on the attainment of adequate binding affinity to specific target receptors associated with each respective pathological condition. Consequently, it is anticipated that these findings will serve as a foundation for future scientific endeavours, encompassing both in vitro and in vivo studies, as well as clinical investigations, to better understand the pharmacological effects of α-mangostin.
    MeSH terms: Animals; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/chemistry; Antioxidants/pharmacology; Antioxidants/chemistry; Humans
  5. Zeng GH, Zhong W, Mazzon G, Zhu W, Lahme S, Khadgi S, et al.
    Mil Med Res, 2024 Oct 28;11(1):70.
    PMID: 39465407 DOI: 10.1186/s40779-024-00562-3
    Over the past three decades, there has been increasing interest in miniaturized percutaneous nephrolithotomy (mPCNL) techniques featuring smaller tracts as they offer potential solutions to mitigate complications associated with standard PCNL (sPCNL). However, despite this growing acceptance and recognition of its benefits, unresolved controversies and acknowledged limitations continue to impede widespread adoption due to a lack of consensus on optimal perioperative management strategies and procedural tips and tricks. In response to these challenges, an international panel comprising experts from the International Alliance of Urolithiasis (IAU) took on the task of compiling an expert consensus document on mPCNL procedures aimed at providing urologists with a comprehensive clinical framework for practice. This endeavor involved conducting a systematic literature review to identify research gaps (RGs), which formed the foundation for developing a structured questionnaire survey. Subsequently, a two-round modified Delphi survey was implemented, culminating in a group meeting to generate final evidence-based comments. All 64 experts completed the second-round survey, resulting in a response rate of 100.0%. Fifty-eight key questions were raised focusing on mPCNLs within 4 main domains, including general information (13 questions), preoperative work-up (13 questions), procedural tips and tricks (19 questions), and postoperative evaluation and follow-up (13 questions). Additionally, 9 questions evaluated the experts' experience with PCNLs. Consensus was reached on 30 questions after the second-round survey, while professional statements for the remaining 28 key questions were provided after discussion in an online panel meeting. mPCNL, characterized by a tract smaller than 18 Fr and an innovative lithotripsy technique, has firmly established itself as a viable and effective approach for managing upper urinary tract stones in both adults and pediatrics. It offers several advantages over sPCNL including reduced bleeding, fewer requirements for nephrostomy tubes, decreased pain, and shorter hospital stays. The series of detailed techniques presented here serve as a comprehensive guide for urologists, aiming to improve their procedural understanding and optimize patient outcomes.
  6. Taha BA, Addie AJ, Haider AJ, Kadhim AC, Azzahrani AS, Arsad N
    Langmuir, 2024 Oct 26.
    PMID: 39460975 DOI: 10.1021/acs.langmuir.4c03513
    This work explores bubble laser technology as an alternative to needles in injection systems for vaccination, cancer treatment, insulin delivery, and catheter hygiene. The technology leverages laser-induced microfiltration and bubble dynamics to create high-speed pneumatic jets that penetrate the skin without needles, addressing discomfort, infection risk, and needle-related concerns. The system's performance is analyzed based on laser wavelength, pulse duration, and Gaussian beam droplet size. The findings indicate a significant increase in spot size at 1064 nm compared with 400 nm, consistent with the diffraction theory. Induced bubble dynamics reveal bubble generation, jetting, and fluid interactions as the Weber number increases, as well as jet velocity and fluid inertia. For femtosecond pulses, increasing the pulse duration from 100 to 1500 fs reduces the bubble lifespan from 0.8 to 0.3 arbitrary units, and the collapse pressure decreases from 2.1 to 0.4 bar. For picosecond pulses, the bubble lifetime decreases from 0.9 to 0.5 arbitrary units, and the pressure drop decreases from 2.0 to 0.4 bar as the pulse length extends from 2000 to 8000 ps. Jet formation in laser jet injection systems is enhanced by short pulses in water that produce longer-lasting bubbles. Drug delivery based on the Rayleigh-Plesset equation is characterized by a low-pressure collapse and short bubble lifetime. Thus, this relationship suggests that bubble laser technology can provide a more controlled and safer method of needle-free procedures, increasing compliance and reducing tissue trauma.
  7. Song J, He X, Zhang F, Wang W, Chan NW, Shi J, et al.
    PLoS One, 2024;19(10):e0312388.
    PMID: 39453961 DOI: 10.1371/journal.pone.0312388
    With the rapid economic development of Xinjiang Uygur Autonomous Region (Xinjiang), energy consumption became the primary source of carbon emissions. The growth trend in energy consumption and coal-dominated energy structure are unlikely to change significantly in the short term, meaning that carbon emissions are expected to continue rising. To clarify the changes in energy-related carbon emissions in Xinjiang over the past 15 years, this paper integrates DMSP/OLS and NPP/VIIRS data to generate long-term nighttime light remote sensing data from 2005 to 2020. The data is used to analyze the distribution characteristics of carbon emissions, spatial autocorrelation, frequency of changes, and the standard deviation ellipse. The results show that: (1) From 2005 to 2020, the total carbon emissions in Xinjiang continued to grow, with noticeable urban additions although the growth rate fluctuated. In spatial distribution, non-carbon emission areas were mainly located in the northwest; low-carbon emission areas mostly small and medium-sized towns; and high-carbon emission areas were concentrated around the provincial capital and urban agglomerations. (2) There were significant regional differences in carbon emissions, with clear spatial clustering of energy consumption. The clustering stabilized, showing distinct "high-high" and "low-low" patterns. (3) Carbon emissions in central urban areas remained stable, while higher frequencies of change were seen in the peripheral areas of provincial capitals and key cities. The center of carbon emissions shifted towards southeast but later showed a trend of moving northwest. (4) Temporal and spatial variations in carbon emissions were closely linked to energy consumption intensity, population size, and economic growth. These findings provided a basis for formulating differentiated carbon emission targets and strategies, optimizing energy structures, and promoting industrial transformation to achieve low-carbon economic development in Xinjiang.
    MeSH terms: Air Pollutants/analysis; China; Remote Sensing Technology*; Spatio-Temporal Analysis
  8. Looi CK, Loo EM, Lim HC, Chew YL, Chin KY, Cheah SC, et al.
    Front Immunol, 2024;15:1484535.
    PMID: 39450176 DOI: 10.3389/fimmu.2024.1484535
    Nasopharyngeal carcinoma (NPC) is a distinct malignancy of the nasopharynx and is consistently associated with the Epstein-Barr virus (EBV) infection. Its unique anatomical location and complex aetiology often result in advanced-stage disease at first diagnosis. While radiotherapy (RT) and chemotherapy have been the mainstays of treatment, they often fail to prevent tumour recurrence and metastasis, leading to high rates of treatment failure and mortality. Recent advancement in cell-based therapies, such as chimeric antigen receptor (CAR)-T cell therapy, have shown great promise in hematological malignancies and are now being investigated for NPC. However, challenges such as targeting specific tumour antigens, limited T cell persistence and proliferation, and managing treatment-related toxicities must be addressed. Extensive research is needed to enhance the effectiveness and safety of these therapies, paving the way for their integration into standard clinical practice for better management of NPC and a better quality of life for human health.
    MeSH terms: Animals; Herpesvirus 4, Human; Genetic Engineering; Humans; Epstein-Barr Virus Infections/immunology; Epstein-Barr Virus Infections/therapy; Stem Cell Transplantation; Cell- and Tissue-Based Therapy/methods
  9. Shokrak NM, Khairi N, Hazrin-Chong NH, Mohamed RA, Abdella B
    Sci Rep, 2024 Oct 23;14(1):25019.
    PMID: 39443501 DOI: 10.1038/s41598-024-74534-x
    Aquaculture is an important component of the world food supply and a significant source of protein. However, this industry faces numerous problems. Including poor fish feed digestion and uneconomic nutrient utilization. This can result in unsatisfactory growth rates and poor stock performance. Utilizing probiotics, which are beneficial microbes that can enhance digestive systems and general fish health, is one possible way to address these issues. This study was designed to identify and evaluate a novel strain of Bacillus as a promising probiotic. The strain of Bacillus rugosus that was examined and coded NM007 showed promising probiotic characteristics that could help fish digest and utilize their feed more efficiently, reduce feed waste, and improve their digestive systems. B. rugosus NM007 exhibited the ability to produce digestive enzymes like protease, amylase, and lipase, which are the main digestive enzymes. It showed strong auto-aggregation activity and co-aggregation activity with Aeromonas sp. and Streptococcus sp. It also demonstrated tolerance to the presence of bile salt, acidic pH, and salinity up to 60 ppt. The sensitivity analysis towards antibiotics, hemolytic activity and the safety assessment on Nile tilapia fish (Oreochromis niloticus) confirmed the safety of this isolate. Based on the findings of this investigation and the isolate's characterization, Bacillus rugosus NM007 could serve as a new promising probiotic bacterium for aquaculture.
  10. Wong PK, Syafruddin SE, Cheah FC, Azmi N, Ng PY, Chua EW
    Per Med, 2024;21(6):353-366.
    PMID: 39440484 DOI: 10.1080/17410541.2024.2412514
    Aim: Rs16851030, a single-nucleotide variant located in the 3'-untranslated region of the ADORA1 gene, has been proposed as a potential marker of caffeine sensitivity in apnea of prematurity. Besides, it is associated with aspirin-induced asthma and the development of acute chest syndrome. However, its functional significance is still unconfirmed. This study aimed to elucidate the functional impact of rs16851030 by using CRISPR/Cas9 approach to induce the DNA variant and attendant physiological changes.Methods: Rs16851030 was introduced into HEK293 cells via homology-directed repair (HDR). Edited cells were fluorescence-enriched, sorted, isolated, and expanded into single-cell-derived clones. The edit was confirmed by Sanger sequencing. RNA sequencing was used to analyze affected pathways.Results: Rs16851030-mutant cells showed increased susceptibility to hypoxia, a condition related to apnea of prematurity. After 24 h of hypoxia, the viability of mutant clones 1 and 2 was low compared with wild-type cells (75.45% and 74.47% vs. 96.34%). RNA sequencing revealed transcriptomic changes linked to this increased vulnerability.Conclusion: Rs16851030 impairs cellular resistance to hypoxia, suggesting its role in conditions like apnea of prematurity. Further research should investigate the molecular mechanisms and transcriptomic alterations caused by rs16851030 under hypoxic conditions.
    MeSH terms: Anoxia/genetics; Caffeine/pharmacology; Humans; Cell Hypoxia/genetics; Genetic Predisposition to Disease/genetics; 3' Untranslated Regions/genetics; HEK293 Cells; Gene Editing/methods
  11. Makki M, Shafie AA, Awaisu A, Hussain R, Al Hail M, ElMotasim WM, et al.
    Explor Res Clin Soc Pharm, 2024 Dec;16:100517.
    PMID: 39435401 DOI: 10.1016/j.rcsop.2024.100517
    BACKGROUND: The COVID-19 outbreak had significant global healthcare implications, including the use of medications. This is specifically evident in the surge in use of some medication and a decline in the use of others. As a result, some medications end up unused, which may have subsequent health, economic, and environmental impacts.

    AIM: To explore patients' insights into unused medications during the COVID-19 outbreak.

    MATERIALS AND METHODS: Semi-structured interviews were conducted with 30 patients attending various public and private healthcare facilities in Qatar between January and July 2021. A thematic analysis approach was utilized, with 2 researchers independently analyzing, comparing, and discussing the coding. The resulting themes were further discussed in research group meetings until a consensus was reached.

    RESULTS: Seven themes emerged: awareness of unused medications and their impacts on health, economy, and the environment; barriers contributing to nonadherence/unused medications; sources of medications; factors facilitating medication use; patients' behaviors toward donated medications/reuse; patients' awareness of medication storage requirements; and patients' desire to appropriately dispose of unused medications.

    CONCLUSION: The COVID-19 outbreak disrupted medication supply and adherence, resulting in an increase in unused medications and inappropriate disposal. Adherence is crucial for improving patients' health and preserving medications. Implementing mail-return systems for unused medications could be a viable solution during disease outbreaks.

  12. Henry Basil J, Lim WH, Syed Ahmad SM, Menon Premakumar C, Mohd Tahir NA, Mhd Ali A, et al.
    Digit Health, 2024;10:20552076241286434.
    PMID: 39430694 DOI: 10.1177/20552076241286434
    OBJECTIVE: Neonates' physiological immaturity and complex dosing requirements heighten their susceptibility to medication administration errors (MAEs), with the potential for severe harm and substantial economic impact on healthcare systems. Developing an effective risk prediction model for MAEs is crucial to reduce and prevent harm.

    METHODS: This national-level, multicentre, prospective direct observational study was conducted in neonatal intensive care units (NICUs) of five public hospitals in Malaysia. Randomly selected nurses were directly observed during medication preparation and administration. Each observation was independently assessed for errors. Ten machine learning (ML) algorithms were applied with features derived from systematic reviews, incident reports, and expert consensus. Model performance, prioritising F1-score for MAEs, was evaluated using various measures. Feature importance was determined using the permutation-feature importance for robust comparison across ML algorithms.

    RESULTS: A total of 1093 doses were administered to 170 neonates, with mean age and birth weight of 33.43 (SD ± 5.13) weeks and 1.94 (SD ± 0.95) kg, respectively. F1-scores for the ten models ranged from 76.15% to 83.28%. Adaptive boosting (AdaBoost) emerged as the best-performing model (F1-score: 83.28%, accuracy: 77.63%, area under the receiver operating characteristic: 82.95%, precision: 84.72%, sensitivity: 81.88% and negative predictive value: 64.00%). The most influential features in AdaBoost were the intravenous route of administration, working hours, and nursing experience.

    CONCLUSIONS: This study developed and validated an ML-based model to predict the presence of MAEs among neonates in NICUs. AdaBoost was identified as the best-performing algorithm. Utilising the model's predictions, healthcare providers can potentially reduce MAE occurrence through timely interventions.

  13. Li Y, Hu J, Hou J, Lu S, Xiong J, Wang Y, et al.
    Sci Rep, 2024 Oct 19;14(1):24595.
    PMID: 39427096 DOI: 10.1038/s41598-024-76506-7
    The spread of SARS-CoV-2 to animals has the potential to evolve independently. In this study, we distinguished several sentinel animal species and genera for monitoring the re-emergence of COVID-19 or the new outbreak of COVID-19-like disease. We analyzed SARS-CoV-2 genomic data from human and nonhuman mammals in the taxonomic hierarchies of species, genus, family and order of their host. We find that SARS-CoV-2 carried by domestic dog (Canis lupus familiaris), domestic cat (Felis catus), mink (Neovison vison), and white-tailed deer (Odocoileus virginianus) cluster closely to human-origin viruses and show no differences in the majority of amino acids, but have the most positively selected sites and should be monitored to prevent the re-emergence of COVID-19 caused by novel variants of SARS-CoV-2. Viruses from the genera Panthera (especially lion (Panthera leo)), Manis and Rhinolophus differ significantly from human-origin viruses, and long-term surveillance should be undertaken to prevent the future COVID-19-like outbreaks. Investigation of the variation dynamics of sites 142, 501, 655, 681 and 950 within the S protein may be necessary to predict the novel animal SARS-CoV-2 variants.
    MeSH terms: Animals; Cats; Dogs; Humans; Mink/virology; Phylogeny; Genome, Viral; Sentinel Species/genetics
  14. Ma Y, Yuan X, Wei A, Li X, Patar A, Su S, et al.
    Redox Biol, 2024 Oct 05;77:103376.
    PMID: 39423458 DOI: 10.1016/j.redox.2024.103376
    The significance of protein S-palmitoylation in angiogenesis has been largely overlooked, leaving various aspects unexplored. Recent identification of Gpx1 as a palmitoylated protein has generated interest in exploring its potential involvement in novel pathological mechanisms related to angiogenesis. In this study, we demonstrate that Gpx1 undergoes palmitoylation at cysteine-76 and -113, with PPT1 playing a crucial role in modulating the depalmitoylation of Gpx1. Furthermore, we find that PPT1-regulated depalmitoylation negatively impacts Gpx1 protein stability. Interestingly, inhibiting Gpx1 palmitoylation, either through expression of a non-palmitoylated Gpx1 mutant or by expressing PPT1, significantly enhances neovascular angiogenesis. Conversely, in PPT1-deficient mice, angiogenesis is notably attenuated compared to wild-type mice in an Oxygen-Induced Retinopathy (OIR) model, which mimics pathological angiogenesis. Physiologically, under hypoxic conditions, Gpx1 palmitoylation levels are drastically reduced, suggesting that increasing Gpx1 palmitoylation may have beneficial effects. Indeed, enhancing Gpx1 palmitoylation by inhibiting PPT1 with DC661 effectively suppresses retinal angiogenesis in the OIR disease model. Overall, our findings highlight the pivotal role of protein palmitoylation in angiogenesis and propose a novel mechanism whereby the PPT1-Gpx1 axis modulates angiogenesis, thereby providing a potential therapeutic strategy for targeting PPT1 to combat angiogenesis.
  15. Beschastnov VV, Shirokova IY, Belyanina NA, Pogodin IE, Tulupov AA, Tochilina AG, et al.
    Sovrem Tekhnologii Med, 2024;16(1):45-52.
    PMID: 39421627 DOI: 10.17691/stm2024.16.1.05
    The aim of the investigation is to study the possibility of applying commercial wound coatings for treating infected wounds as a carrier matrix for bacteriophages.

    MATERIALS AND METHODS: Twelve varieties of commercial wound coverings based on biopolymers of natural and synthetic origin, a biological preparation Staphylophag produced by scientific-industrial association Microgen (Russia), registration certificate P N001973/01, and the S. aureus 3196 test strain (GenBank JARQZO000000000) isolated from a patient with a burn wound have been used in our work. The ability of commercial biological wound coatings to absorb solutions was examined by immersing them in a physiological solution (pH 7.0-7.2) followed by weighing. The lytic activity of three bacteriophage series against the test strain was studied using the Appelman method and a spot test. The lytic activity of the bacteriophage in the wound samples was studied within 7 days after its absorption by the wound coatings.

    RESULTS: The greatest volume of fluid was absorbed by the LycoSorb, NEOFIX FibroSorb Ag, Biatravm, and Chitocol-S wound coatings. All bacteriophage series have been found to have a high lytic activity against the test strain. It has also been shown that Chitocol-S, Collachit-FA, Algipran, and Aquacel Ag Extra possessed their own inherent antibacterial activity under in vitro conditions stable for 7 days; moreover, the lysis zones of the test strain increased after their saturation with bacteriophage. On day 0, a high level of bacteriophage lytic activity with the maximum size of the test strain lysis zones from 49 to 59 mm have been found to remain in all samples of the wound coverings. The bacteriophage activity persisted for 1 day in the samples of Hydrofilm, Polypran, and NEOFIX FibroCold Ag coatings, up to 4 days in Algipran, Nano-Aseptica, and Biatravm coatings; and for 7 days in the Chitocol-S, Collachit-FA, Opsite Post-Op Visible, NEOFIX FibroSorb Ag, Aquacel Ag Extra, and LycoSorb samples.

    CONCLUSION: Modern commercial wound dressings based on chitosan-collagen complex (Chitocol-S, Collachit-FA), polyurethane (Opsite Post-Op Visible, LycoSorb, NEOFIX FibroSorb Ag), and Hydrofiber (Aquacel Ag Extra) have a sufficient level of bacteriophage solution absorption, provide a stable preservation of the bacteriophage lytic activity under in vitro conditions up to 7 days. Thus, the in vitro studies prove the possibility of their use as a carrier matrix for bacteriophages.

    MeSH terms: Bacteriophages*; Bandages; Feasibility Studies; Humans; Staphylococcus aureus/drug effects; Staphylococcus aureus/virology; Wound Healing; Phage Therapy
  16. Bala Ganesh KA, Panda P, Gurawa T, Gopalakrishna PK, Jagadeesan S, Vishnumukkala T
    Bioinformation, 2024;20(8):872-876.
    PMID: 39411772 DOI: 10.6026/973206300200872
    Anatomy is a vital discipline in the realm of Medicine, and its primary means of study is through the use of cadavers. Experts in the medical, legal, and ethical domains have discussed the use of cadavers or their anatomical parts for educational purposes, which are considered a severe drawback. The advantages of using cadavers include their contribution to medical education, research, the investigation of innovative surgical procedures or techniques, the detection of anatomical differences at an individual or population level, the enhancement of surgical skills, and the support of other anatomical investigations. This review highlights the issues like consent, respect for the deceased and cultural beliefs on cadaver procurement practices and also it emphasizes the need for better body donation initiatives and public awareness campaigns to ensure sustainable cadaver procurement practices, ensuring consent, respect for the deceased and cultural beliefs.
  17. Sunaiwi R, Gaur R, Azhar Abdul Razab MK, Hadzuan FH, Nawi NM, Abdul Aziz MZ, et al.
    Heliyon, 2024 Oct 15;10(19):e38682.
    PMID: 39403514 DOI: 10.1016/j.heliyon.2024.e38682
    Patients undergoing high-dose radioiodine ablation (RAI) therapy in Nuclear Medicine Department need to be isolated in a special designed ward for a few days. Large amount of clinical radioactive wastewater from patient body is produced during high-activity RAI therapy. The radioactive wastewater needs to store in a delay tank until the radioactivity decayed below acceptable limit before being discharged and indirectly limit the patient admission and treatment. This study is to propose an alternative antibacterial adsorbent for I-131 extraction from clinical radioactive wastewater at the nuclear medicine department using graphene oxide silver (GOAg) and bamboo activated carbon (BAC). The synthesised adsorbents and their sediments (filtered sample) were analysed using field emission scanning electron microscopy (FESEM) for morphological analysis and analysed using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) and X-ray diffraction (XRD). XPS spectra for C 1s adsorbents show intensity peaks at 284.45 eV (C=C) and 285.3 eV (C-C) for GOAg and its sediments, and 284.35 eV (C-C), 287.00 eV (C=O), and 290.07 eV (π-π∗ transitions) for BAC and its sediments. FTIR spectra reveal various functional groups of adsorbents: C=C (1637.50772 cm-1), C=O (1340.48041 cm-1), and C-O-C (1031.88060 cm-1) for GOAg and its sediments, and C=C (1635.57897 cm-1), C-C (1257.54421 cm-1), and C-O (1188.10925 cm-1) for BAC and its sediments. XRD patterns exhibit peaks at 2θ = 27.82°, 29.39°, 32.24°, and 46.22°, which can be attributed to the (002) diffraction plane, (220) crystallographic plane, (111) plane of Ag2O, and (200) crystallographic plane, respectively, for GOAg and its sediments. Meanwhile, the peaks at 2θ = 26.56° and 42.41°, which correspond to (002) and (100) planes, respectively, for BAC and its sediments. The d-spacing and the crystallinity index of each adsorbent were also determined. The estimation of the remaining β- particles during the adsorption of I-131 was carried out using PHITS. The finding of this study is beneficial for alternative radionuclide extractions technique from clinical radioactive wastewater in nuclear medicine.
  18. Khazali AS, Hadrawi WH, Ibrahim F, Othman S, Nor Rashid N
    Expert Rev Mol Med, 2024 Oct 14;26:e26.
    PMID: 39397710 DOI: 10.1017/erm.2024.18
    Thrombocytopenia is a common symptom and one of the warning signs of dengue virus (DENV) infection. Platelet depletion is critical as it may lead to other severe dengue symptoms. Understanding the molecular events of this condition during dengue infection is challenging because of the multifaceted factors involved in DENV infection and the dynamics of the disease progression. Platelet levels depend on the balance between platelet production and platelet consumption or clearance. Megakaryopoiesis and thrombopoiesis, two interdependent processes in platelet production, are hampered during dengue infection. Conversely, platelet elimination via platelet activation, apoptosis and clearance processes are elevated. Together, these anomalies contribute to thrombocytopenia in dengue patients. Targeting the molecular events of dengue-mediated thrombocytopenia shows great potential but still requires further investigation. Nonetheless, the application of new knowledge in this field, such as immature platelet fraction analysis, may facilitate physicians in monitoring the progression of the disease.
    MeSH terms: Animals; Dengue Virus*; Humans; Platelet Activation; Apoptosis; Thrombopoiesis
  19. Si S, Huang X, Wang Q, Manickam S, Zhao D, Liu Y
    Int J Biol Macromol, 2024 Nov;281(Pt 4):136505.
    PMID: 39395516 DOI: 10.1016/j.ijbiomac.2024.136505
    Microbial contamination annually leads to substantial food resource loss. Effective food packaging can mitigate food contamination and waste, yet conventional materials such as plastics often lack bacteriostatic activity. This study aimed to synthesise FengycinA-M3@bacterial cellulose@polyvinyl alcohol composite hydrogels via dual cross-linking with hydrogen and borate bonding, with the goal of enhancing antibacterial properties and prolonging the preservation period of refrigerated chicken breast. The composite hydrogel was subjected to comprehensive characterisation for structural, mechanical, water absorption, slow peptide release, antimicrobial capacity, biocompatibility, and chicken breast freshness preservation. The results showed that the composite hydrogel had a porous network structure and excellent gel elasticity and biocompatibility. It was effective in inhibiting Staphylococcus aureus and Escherichia coli, and prolonged the storage time of frozen chicken breast for up to 12 days. These findings emphasise the potential of hydrogel food packaging to prolong storage periods and its suitability for food industry applications due to ease of manufacture.
    MeSH terms: Animals; Anti-Bacterial Agents/pharmacology; Anti-Bacterial Agents/chemistry; Chickens*; Escherichia coli/drug effects; Food Preservation/methods; Refrigeration; Staphylococcus aureus/drug effects
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