Browse publications by year: 2025

  1. Zàaba NF, Ogaili RH, Ahmad F, Mohd Isa IL
    Spine J, 2025 Jan 13.
    PMID: 39814205 DOI: 10.1016/j.spinee.2024.12.033
    Intervertebral disc (IVD) degeneration is a major cause of low back pain (LBP), which results in disability worldwide. However, the pathogenesis of IVD degeneration mediating LBP remains unclear. Current conservative treatments and surgical interventions are both to relieve the symptoms and minimise pain; nevertheless, they are unable to reverse the degeneration. Previous studies have shown that inflammation and nociception markers are important indicators of pain mechanisms in IVD degeneration underlying LBP. As such, multiomics profiling allows the discovery of these target markers to understand the key pathological mechanisms mediating IVD degeneration underpinnings of LBP. This article provides insights into a precision medicine approach for identifying and understanding the pathophysiology of IVD degeneration associated with LPB based on the severity of the disease from early and mild to severe degenerative stages. Molecular profiling of key markers in degenerative IVDs based on patient stratification at early, mild, and severe stages will contribute to the identification of target markers associated with signalling pathways in mediating neuroinflammation, innervation, and nociception underlying painful IVD degeneration. This approach will offer an understanding of establishing personalised clinical strategies tailored to the severity of IVD degeneration for the treatment of LBP.
  2. Miow YX, Gan WY, Fahmida U, Lim PY, Appannah G
    Food Nutr Bull, 2025 Jan 15.
    PMID: 39814526 DOI: 10.1177/03795721241302864
    BACKGROUND: Poor diet quality may contribute to undernutrition, particularly affecting young children from low-income households. Therefore, affordable and healthy local food-based recommendations (FBRs) are needed.

    OBJECTIVES: This study aimed to (1) identify problem nutrient(s), (2) identify locally available nutrient-dense food sources that can provide adequate nutrients to meet the recommended dietary intakes of undernourished urban poor children aged 2-5 years, and (3) use linear programming to recommend a daily diet based on the current food patterns that achieves dietary adequacy and meets average food costs for these children.

    METHODS: A cross-sectional study was conducted in Seremban, Malaysia. Three-day 24hour dietary recalls were collected from the mothers (n = 168). Food costs were estimated using market surveys. The FBRs were formulated by linear programming analysis using Optifood software.

    RESULTS: Folate was an "absolute problem nutrient" (such a nutrient does not achieve the 100% recommended nutrient intake even upon diet optimization). Full-cream milk, chocolate malted milk, fortified cornflakes, and chicken egg were the common locally available nutrient-dense foods that would provide adequate nutrients to meet the recommended dietary intakes of undernourished urban poor children. The final FBR set would ensure dietary adequacy for eight to nine of 11 nutrients at a cost of ringgit Malaysia (RM) 4.80/day (US $1.05/day) and RM6.20/day (US $1.34/day), depending on the age group.

    CONCLUSIONS: Interventions are required to ensure dietary adequacy for undernourished urban poor children under 5 years old. Dietary adequacy could be improved via realistic changes in habitual food consumption practices.

  3. Wong YY, Yap JS, Chu SY, Smith G, Woi PJ, Chai SC, et al.
    Child Care Health Dev, 2025 Jan;51(1):e70038.
    PMID: 39814563 DOI: 10.1111/cch.70038
    BACKGROUND AND OBJECTIVES: Some families faced difficulties accessing speech therapy in some areas of Malaysia and/or during the COVID-19 lockdown. Therefore, parent-mediated intervention could be one of the ways to solve this issue, as it has been proven to effectively improve communication skills among children with communication disorders. Hence, a Mandarin parental guidebook comprising a series of language stimulation activities was developed to explore the perception of parents of children with communication disorders, ranging from 3 to 6 years old, regarding the feasibility of (1) conducting speech-language home programs and (2) using a home-based parental guidebook as a tool to deliver a speech-language home program.

    DESIGN, SETTING AND PARTICIPANTS: Semistructured interviews were conducted with nine Malaysian parents of child (ren) with communication disorders, who fulfilled the inclusionary criteria. Parents had attempted the speech-language home program by using the guidebook prior to the interview. The participants were subjected to 45-min to 1-h interviews. The interviews were audio- or video-recorded for verbatim transcription. Thematic analysis was used for data interpretation.

    RESULTS: Four main themes were identified: (1) 'Golden Period': a guidebook to deliver speech-language home program, (2) perception of the speech-language home program among parents of children with communication disorders, (3) challenges faced by parents when practicing speech-language home program and (4) suggestions for improvements: parent's needs. The themes informed the perspective of parents towards the user experience of the parental guidebook, the feasibility of the speech-language home program and their recommendations. Overall, participants conveyed positive responses on the parental guidebook.

    CONCLUSIONS: Malaysian parents face difficulties in accessing speech therapy because of limited numbers of SLTs, geographical barriers, financial constraints, availability of facilities and so forth. Findings could assist SLTs in adopting a family-centred approach in their service delivery, thus increasing the cost-effectiveness of their service delivery.

    MeSH terms: Adult; Child; Child, Preschool; Communication Disorders/rehabilitation; Female; Humans; Language Therapy; Malaysia; Male; Speech Therapy; Qualitative Research
  4. Eachempati P, Komattil R, Arakala A
    Adv Physiol Educ, 2025 Mar 01;49(1):208-209.
    PMID: 39814611 DOI: 10.1152/advan.00191.2024
    As artificial intelligence (AI) continues to reshape education, concerns about the authenticity of student work have escalated, particularly in relation to written assignments influenced by AI-powered tools. This article explores the role of the oral examination as a valuable method for assessing true student understanding and considers its potential for broader use across various educational levels. We propose that oral exams could be effectively integrated into undergraduate courses, providing a means to verify student comprehension in an era of AI-generated content. By adopting oral assessments, educators can enhance academic integrity while fostering essential skills such as critical thinking and effective communication in today's technology-driven world.
    MeSH terms: Humans; Physiology/education
  5. Cheng C, Aruchunan E, Noor Aziz MH
    Sci Rep, 2025 Jan 15;15(1):2043.
    PMID: 39814760 DOI: 10.1038/s41598-025-85440-1
    A dynamics informed neural networks (DINNs) incorporating the susceptible-exposed-infectious-recovered-vaccinated (SEIRV) model was developed to enhance the understanding of the temporal evolution dynamics of infectious diseases. This work integrates differential equations with deep neural networks to predict time-varying parameters in the SEIRV model. Experimental results based on reported data from China between January 1, and December 1, 2022, demonstrate that the proposed dynamics informed neural networks (DINNs) method can accurately learn the dynamics and predict future states. Our proposed hybrid SEIRV-DNNs model can also be applied to other infectious diseases such as influenza and dengue, with some modifications to the compartments and parameters in the model to accommodate the related control measures. This approach will facilitate improving predictive modeling and optimizing public health intervention strategies.
    MeSH terms: China/epidemiology; Humans; Neural Networks (Computer)*
  6. Hai T, Basem A, Alizadeh A, Singh PK, Rajab H, Maatki C, et al.
    Sci Rep, 2025 Jan 15;15(1):1986.
    PMID: 39814861 DOI: 10.1038/s41598-025-85236-3
    The performance of nanofluids is largely determined by their thermophysical properties. Optimizing these properties can significantly enhance nanofluid performance. This study introduces a hybrid strategy based on computational intelligence to determine the optimal conditions for ternary hybrid nanofluids. The goal is to minimize dynamic viscosity and maximize thermal conductivity by varying the volume fraction, temperature, and nanomaterial mixing ratio. The proposed strategy integrates machine learning, multi-objective optimization, and multi-criteria decision-making. Three machine learning techniques-GMDH-type neural network, gene expression programming, and combinatorial algorithm-are applied to model dynamic viscosity and thermal conductivity as functions of the input variables. Then, the high-performing models provide the foundation for optimization using the well-established multi-objective particle swarm optimization algorithm. Finally, the decision-making technique TOPSIS is employed to identify the most desirable points from the Pareto front, based on various design scenarios. To validate the proposed strategy, a ternary hybrid nanofluid composed of graphene oxide (GO), iron oxide (Fe₃O₄), and titanium dioxide (TiO₂) was employed as a case study. The results demonstrated that the combinatorial approach excelled in accurately modeling (R = 0.99964-0.99993). The optimization process revealed that optimal VFs span a broad range across all mixing ratios, while optimal temperatures were consistently near the maximum value (65 °C). The decision-making outcomes indicated that the mixing ratio was consistent across all design scenarios, with the volume fraction serving as the key differentiating factor.
  7. Basurto X, Gutierrez NL, Franz N, Mancha-Cisneros MDM, Gorelli G, Aguión A, et al.
    Nature, 2025 Jan;637(8047):875-884.
    PMID: 39814892 DOI: 10.1038/s41586-024-08448-z
    Sustainable development aspires to "leave no one behind"1. Even so, limited attention has been paid to small-scale fisheries (SSF) and their importance in eradicating poverty, hunger and malnutrition. Through a collaborative and multidimensional data-driven approach, we have estimated that SSF provide at least 40% (37.3 million tonnes) of global fisheries catches and 2.3 billion people with, on average, 20% of their dietary intake across six key micronutrients essential for human health. Globally, the livelihood of 1 in every 12 people, nearly half of them women, depends at least partly on small-scale fishing, in total generating 44% (US$77.2 billion) of the economic value of all fisheries landed. Regionally, Asian SSF provide fish, support livelihoods and supply nutrition to the largest number of people. Relative to the total capture of the fisheries sector (comprising large-scale and small-scale fisheries), across all regions, African SSF supply the most catch and nutrition, and SSF in Oceania improve the most livelihoods. Maintaining and increasing these multidimensional SSF contributions to sustainable development requires targeted and effective actions, especially increasing the engagement of fisherfolk in shared management and governance. Without management and governance focused on the multidimensional contributions of SSF, the marginalization of millions of fishers and fishworkers will worsen.
    MeSH terms: Animals; Diet/veterinary; Female; Fishes; Food Supply/economics; Food Supply/statistics & numerical data; Humans; Male; Micronutrients/analysis; Malnutrition/epidemiology; Malnutrition/prevention & control
  8. Puan SL, Erriah P, Yahaya NM, Ali MSM, Ahmad SA, Oslan SN, et al.
    PMID: 39815115 DOI: 10.1007/s12602-025-10451-3
    The emergence of multidrug-resistant pathogens presents a significant global health challenge, which is primarily fuelled by overuse and misuse of antibiotics. Bacteria-derived antimicrobial metabolites offer a promising alternative strategy for combating antimicrobial resistance issues. Bacillus velezensis PD9 (BvPD9), isolated from stingless bee propolis, has been reported to have antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to characterise and identify the antimicrobial compounds (AMCs) synthesised by BvPD9 through integration of genome mining and liquid chromatography-mass spectrometry (LC-MS) analysis. The whole-genome sequence of BvPD9 contained 4,263,351 base pairs and 4101 protein-coding sequences, with 12 potential AMC biosynthetic gene clusters. Comparative genomic analysis highlighted the unique profile of BvPD9 that possesses the largest number of unknown proteins, indicating significant potential for further exploration. The combined genomics-metabolic profiling uncovered five AMCs in BvPD9 extract, including bacillibactin, bacilysin, surfactin A, fengycin A, and bacillomycin D. The extract exhibited a broad antibacterial spectrum against 25 pathogenic bacteria, including both Gram-positive and Gram-negative bacteria, with the lowest minimum inhibitory concentration (MIC, 0.032 mg/ml) against S. epidermidis ATCC 12228, and the lowest minimum bactericidal concentration (MBC; 0.128 mg/ml) against MRSA ATCC 700699 and Aeromonas hydrophilia. The robust stability of BvPD9 extract was demonstrated at high temperatures, over a wide range of pH conditions (6 to 12) and in the presence of various hydrolytic enzymes. Additionally, the extract showed 50% haemolytic and cytotoxicity activity at 0.158 and 0.250 mg/ml, respectively. These characteristics suggest potential applications of BvPD9 metabolites for tackling antimicrobial resistance and its applicability across diverse industries.
  9. Low CY, Gan WL, Lai SJ, Tam RS, Tan JF, Dietl S, et al.
    J Nanobiotechnology, 2025 Jan 15;23(1):16.
    PMID: 39815320 DOI: 10.1186/s12951-024-03062-7
    Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by insulin resistance, leading to elevated blood sugar levels. Exogenous insulin can counteract the diminished response to insulin and effectively controlling blood glucose levels, thereby minimizing diabetes-related complications. However, given the injectable nature of exogenous insulin, apprehensions regarding its safety and the difficulties associated with its administration have hindered its widespread and prompt utilization. In this context, advanced oral insulin formulations can improve medication adherence in patients with diabetes and enhance their quality of life. Over the last 20 years, sophisticated pharmaceutical technologies have been utilized to provide insulin through oral formulations. Despite the limited absorption of oral insulin, these studies have demonstrated encouraging outcomes in translating clinical discoveries into commercialization. This review examines the advancements of several oral insulin formulations in preclinical and clinical trials, their effectiveness and safety characteristics, and potential implications for future treatment options.
    MeSH terms: Administration, Oral; Animals; Blood Glucose/drug effects; Humans
  10. Jimoh KA, Hashim N, Shamsudin R, Man HC, Jahari M, Megat Ahmad Azman PN, et al.
    Curr Res Food Sci, 2025;10:100963.
    PMID: 39817041 DOI: 10.1016/j.crfs.2024.100963
    This study detected the macronutrients retained in glutinous rice (GR) under different drying conditions by innovatively applying visible-near infrared hyperspectral imaging coupled with different spectra preprocessing and effective wavelength selection techniques (EWs). Subsequently, predictive models were developed based on processed spectra for the detection of the macronutrients, which include protein content (PC), moisture content (MC), fat content (FC), and ash content (AC). The result shows the raw spectra-based model had a prediction accuracy ( R p 2 ) of 0.6493, 0.9521, 0.4594, and 0.9773 for PC, MC, FC, and AC, respectively. Applying Savitzky Golay first derivatives (SG1D) method increases the R p 2 value to 0.9972, 0.9970, 0.9857 and 0.9972 for PC, MC, FC, and AC, respectively. Using the variable iterative space shrinkage algorithm (VISSA) as EWs reduces the spectral bands by over 60%, and this increases the accuracy of the model (SG1D-VISSA-PLSR) to 100%. Therefore, the developed SGID-VISSA-PLSR can be used to build a smart and reliable spectral system for detecting the macronutrients in GR grains.
  11. Burud IA, Elhariri S, Eid N
    World J Gastrointest Oncol, 2025 Jan 15;17(1):99994.
    PMID: 39817130 DOI: 10.4251/wjgo.v17.i1.99994
    Gallbladder carcinoma (GBC) is the most common malignant tumor of biliary tract, with poor prognosis due to its aggressive nature and limited therapeutic options. Early detection of GBC is a major challenge, with most GBCs being detected accidentally during cholecystectomy procedures for gallbladder stones. This letter comments on the recent article by Deqing et al in the World Journal of Gastrointestinal Oncology, which summarized the various current methods used in early diagnosis of GBC, including endoscopic ultrasound (EUS) examination of the gallbladder for high-risk GBC patients, and the use of EUS-guided elastography, contrast-enhanced EUS, trans-papillary biopsy, natural orifice transluminal endoscopic surgery, magnifying endoscopy, choledochoscopy, and confocal laser endomicroscopy when necessary for early diagnosis of GBC. However, there is a need for novel methods for early GBC diagnosis, such as the use of artificial intelligence and non-coding RNA biomarkers for improved screening protocols. Additionally, the use of in vitro and animal models may provide critical insights for advancing early detection and treatment strategies of this aggressive tumor.
  12. El-Akabawy G, Eid N
    World J Diabetes, 2025 Jan 15;16(1):100066.
    PMID: 39817227 DOI: 10.4239/wjd.v16.i1.100066
    Diabetes mellitus, particularly type 2 diabetes mellitus (T2DM), poses a significant global health challenge. Traditional management strategies primarily focus on glycemic control; however, there is a growing need for comprehensive approaches addressing the complex pathophysiology of diabetes complications. The recent study by Attia et al explores the potential of a novel therapy combining metformin with cholecalciferol (vitamin D3) and taurine to mitigate T2DM-related complications in a rat model. The findings indicate that this treatment combination improves glycemic control and reduces oxidative stress, inflammation, and lipid abnormalities. However, the study is limited by a lack of safety profile data and in-depth molecular mechanism insights. This editorial critically highlights the study's strengths and weaknesses, compares it against other combination therapy research in T2DM, and underscores the need to explore further the mechanisms underpinning the observed therapeutic effects and investigate the safety profile of this novel approach.
  13. Akashanand, Khatib MN, Balaraman AK, Roopashree R, Kaur M, Srivastava M, et al.
    J Asthma, 2025 Feb 01.
    PMID: 39817407 DOI: 10.1080/02770903.2025.2453810
    OBJECTIVE: Asthma poses a significant health burden in South Asia, with increasing incidence and mortality despite a global decline in age-standardized prevalence rates. This study aims to analyze asthma trends from 1990 to 2021, focusing on prevalence, incidence, mortality, and disability-adjusted life years (DALYs) across South Asia. The study also assesses the impact of risk factors like high body mass index (BMI), smoking, and occupational exposures on asthma outcomes.

    METHOD: We extracted asthma data from the Global Burden of Disease database for South Asia (1990-2021). Joinpoint regression analysis was used to assess temporal trends in asthma burden. Total Percentage change (TPC) in age-standardized rates of incidence, mortality, and DALYs were calculated. Data were stratified by gender, and the contribution of risk factors was evaluated.

    RESULTS: Asthma-related mortality in South Asia decreased by 37%, from 27.78 per 100,000 (1990) to 17.54 per 100,000 (2021). The Maldives showed the most significant reduction in mortality (78.31%), while Bangladesh recorded a 47.44% reduction in prevalence and a 62.64% decrease in DALYs. High BMI, smoking, and environmental risks contributed significantly to DALYs, with environmental factors playing a major role in countries like Afghanistan (20.73%) and Bhutan (18.58%). Females, particularly those over 20, experienced higher asthma-related DALYs than males.

    CONCLUSION: Asthma burden in South Asia has reduced over the past three decades, yet the absolute number of cases continues to rise, driven by population growth and environmental risk factors. Targeted interventions addressing risk factors and healthcare disparities are essential for further reducing asthma burden.

  14. Md Najib A, Yusof KM, Wan Shuaib WMA, Shafee MS, Mohd Nor F
    Int J Legal Med, 2025 Jan;139(1):253-265.
    PMID: 39302346 DOI: 10.1007/s00414-024-03328-7
    Postmortem analysis of C-reactive protein (CRP) in autopsies has been extensively researched for its potential utility. This analysis could aid forensic pathologists in screening for and investigating the potential infectious or inflammatory causes of death, thereby guiding appropriate autopsy procedures. To assess the diagnostic accuracy of postmortem CRP analysis in autopsy settings, a thorough electronic literature search was conducted across databases such as PubMed, Scopus, Web of Science, and Cochrane Library. Two independent reviewers screened eligible studies, followed by a methodological quality assessment using the QUADAS-2 checklist. Utilising a random-effects model, hierarchical summary receiver operating characteristic (HSROC) curve analysis and bivariate model meta-analysis were performed to evaluate heterogeneity across studies. Of the 1286 studies initially identified, nine met the eligibility criteria for the final analysis. The pooled sensitivity of postmortem CRP analysis was 0.93 (95% CI, 0.76, 0.98), with a pooled specificity of 0.80 (95% CI, 0.71, 0.87). The prevalence across studies ranged from 0.23 to 0.68, with a median of 0.5. Moderate variability was observed in the heterogeneity assessment across the primary studies. In summary, the study findings indicate that postmortem serum CRP analysis demonstrates high diagnostic accuracy with moderate heterogeneity. Additionally, postmortem CRP testing may be useful as a screening tool in autopsy practice to rule out the likelihood of sepsis.
    MeSH terms: Autopsy*; Humans; Sensitivity and Specificity; Biomarkers/blood
  15. Im JH, Han M, Hong J, Kim H, Oh KS, Choi T, et al.
    ACS Nano, 2025 Jan 14;19(1):1177-1189.
    PMID: 39748139 DOI: 10.1021/acsnano.4c13217
    Perovskite nanocrystals (PNCs) are promising active materials because of their outstanding optoelectronic properties, which are finely tunable via size and shape. However, previous synthetic methods such as hot-injection and ligand-assisted reprecipitation require a high synthesis temperature or provide limited access to homogeneous PNCs, leading to the present lack of commercial value and real-world applications of PNCs. Here, we report a room-temperature approach to synthesize PNCs within a liquid crystalline antisolvent, enabling access to PNCs with a precisely defined size and shape and with reduced surface defects. We demonstrate that elastic strains and long-range molecular ordering of the liquid crystals play a key role in not only regulating the growth of PNCs but also promoting high surface passivation of PNCs with ligands. The approach is a simple, rapid, and room-temperature process, yet it enables access to highly homogeneous PNCs on a mass scale with substantially reduced surface defect states leading to significantly enhanced optoelectronic features. Our results provide a versatile and generalizable strategy to be broadly compatible with a range of nanomaterials and other synthetic methods such as ligand exchange and microfluidic processes.
  16. Queen J, Cing Z, Minsky H, Nandi A, Southward T, Ferri J, et al.
    bioRxiv, 2025 Jan 01.
    PMID: 39803475 DOI: 10.1101/2024.12.30.630810
    Fusobacterium nucleatum is an oral bacterium known to colonize colorectal tumors, where it is thought to play an important role in cancer progression. Recent advances in sequencing and phenotyping of F. nucleatum have revealed important differences at the subspecies level, but whether these differences impact the overall tumor ecology, and tumorigenesis itself, remain poorly understood. In this study, we sought to characterize Fusobacteria in the tumor microbiome of a cohort of individuals with CRC through a combination of molecular, spatial, and microbiologic analyses. We assessed for relative abundance of F. nucleatum in tumors compared to paired normal tissue, and correlated abundance with clinical and pathological features. We demonstrate striking enrichment of F. nucleatum and the recently discovered subspecies animalis clade 2 (Fna C2) specifically in colon tumors that have biofilms, highlighting the importance of complex community partnerships in the pathogenesis of this important organism.
  17. Sapkota B, Pariatamby A
    Environ Sci Pollut Res Int, 2025 Jan;32(5):2577-2591.
    PMID: 39808258 DOI: 10.1007/s11356-024-35817-y
    Wastewater treatment plant (WWTP) is a sustainable technique for making wastewater reusable for non-potable purposes. However, in developing countries, most conventional WWTPs are not equipped to trap all pharmaceutical residues (PRs) and pharmaceutically active chemicals (PhACs). This study aims to perform non-target screening of these contaminants in wastewater and explore health and environmental hazards and the removal efficiency of a WWTP in Malaysia. At Indah Water WWTP, a total of 28 wastewater samples (i.e., 2 L each of 14 influent and 14 effluent) were collected every day for a week from February to April 2023. The supernatant of the centrifuged sample was analyzed with the LCMS-QTOF system. Chromatographic profiles were analyzed, and the compounds were annotated using the METLIN database. Categorical data were statistically analyzed with SPSS 29.0 using a chi-square test and continuous variables using paired t-test and multiple regression. PRs like micronutrient (9, 2.3%) and PhACs like lipid (83, 20.8%) were more frequent. Detection frequencies of PRs and PhACs were 72 (18%) and 328 (82%), respectively. Efficiency of WWTP was 36.4 to 100% for PRs removal (mean ± SD: 65.85 ± 56.43%) and 20 to 100% for PhACs removal (mean ± SD: 49.30 ± 55.94%). A total of 943 (mean ± SD: 67.36 ± 43.28) and 400 (mean ± SD: 28.57 ± 32.44) unique PRs and PhACs were recorded. A total of 40 (10%) PRs and PhACs had the potential to irritate eyes, skin, and respiratory tract, and 46 (11.5%) chemicals needed to be avoided from being discharged into the environment. Though WWTP was 98.0% compliant with environmental standards, its efficiency should still be increased to remove the full range of PRs and PhACs. The research has implications for SDGs 6 and 14.
    MeSH terms: Pharmaceutical Preparations/analysis; Environmental Monitoring; Malaysia; Waste Disposal, Fluid*
  18. Mohd Zainal Abidin Shukri Y, Abd Rahim IN, Abdul Nasir NA, Osman CP, Mohd Kasim NA
    PLoS One, 2025;20(1):e0315178.
    PMID: 39808674 DOI: 10.1371/journal.pone.0315178
    Cataracts are significant causes of blindness, closely linked to prolonged hypercholesterolemia. While saffron has the potential for eye health, its effects on lens lesions remain understudied. This study aimed to investigate the effect of saffron on the lens changes in atherosclerotic-induced New Zealand white rabbits (NZWR). Thirty-five NZWRs were subjected to four to eight weeks of high-cholesterol diet to induce atherosclerosis, resulting in cataractous lens changes. The rabbits were categorised randomly into three groups: normal diet group, pre-treated group and treated group. The pre-treated group was divided into early atherosclerosis(HC4) and established atherosclerosis (HC8). The saffron-treated group was fed with the HCD diet followed by saffron treatment of 50mg/kg/day (TG450, TG840) and 100mg/kg/day (TG4100, TG8100) of saffron ethanolic extract (SEE) respectively. The normal diet group was given a normal diet over the 8 weeks. After completing the 16-week experimental protocol, the NZWR were euthanized, and their lenses were extracted for histopathological evaluation. The pre-treated group exhibited cataractous lens changes of grade 2, characterized by increased homogenisation, swollen lens fibers, and intracellular vacuolisation. Interestingly, these cataract changes showed a positive trend from grade 2 to grade 1 post-treatment with SEE. In the saffron-treated group, vacuoles and pinkish homogenised areas were reduced. Additionally, a uniform layer of anterior epithelium and decreased non-swollen lens fibers indicated significant cataract lesion improvement. The normal diet group displayed minimal to zero cataractous changes (Grade 0). HPLC analysis demonstrated the presence of crocin, crocetin, and picocrocin in the saffron ethanolic extract, with peak absorptions at 440nm (12.817min), 440nm (1.620min), and 254nm (6.553min) respectively. The phytochemical screening of saffron ethanolic extract was conducted and showed the presence of phytochemical compounds including saponins, flavonoids, tannins, and steroids. The positive effects on lenses in the TG groups could be due to crocin and crocetin, bioactive components of saffron, and its phytochemical compounds. This study highlights saffron's potential in managing cataract-induced conditions, emphasizing the importance of further research for its full therapeutic potential in cataract management.
    MeSH terms: Animals; Lens, Crystalline/drug effects; Lens, Crystalline/pathology; Male; Rabbits; Disease Progression
  19. Isa MF, Nazimi AJ, Ramli R
    PMID: 39809363 DOI: 10.1016/j.jormas.2025.102230
    INTRODUCTION: Orbital fractures are common among maxillofacial injuries. The primary objective of this study was to investigate the clinical and radiographic characteristics of pure orbital blowout fractures (OBFs) and their association with post-traumatic diplopia. The secondary objective was to identify predictors of diplopia in pure OBFs.

    METHOD: This retrospective cross-sectional study analysed the clinical records and computed tomography (CT) data of patients with pure OBFs, treated between 1st January 2008 and 28th February 2022 at a single tertiary trauma centre in Klang Valley, Kuala Lumpur, Malaysia. Data collected included demographic characteristics and the aetiology of OBFs. Specific factors such as fracture site, size, type, and soft tissue involvement were assessed using CT images, which were converted to DICOM format and analysed with the OsiriX v4.1.2 software. Diplopia was measured using the Hess area ratio percentage (HAR%).

    RESULTS: The analysis included records of 70 patients. Motor vehicle accidents (MVAs) accounted for 61.4 % of pure OBFs. The largest fracture observed involved both the orbital floor and the medial wall, with a mean size of 6.40 ± 3.05 cm². Among the patients, 41.6 % had Type 3 fractures, and 50 % (n = 35) had 2-point muscle-soft tissue contact. Fracture size, site, type, and muscle contact were all significantly associated with HAR92 % (p < 0.05). Logistic regression revealed that Type 3 fracture was the significant predictor of diplopia (HAR < 92 %), with an odds ratio of 19.80 (95 % CI: 5.76-68.07, p < 0.001).

    CONCLUSION: Fracture site, size, type, and muscle involvement were important factors associated with diplopia. Among these, Type 3 fractures were identified as the primary predictor of HAR < 92 %.

  20. Saheed IO, Ying LR, Yaacob SFFS, Hanafiah MAKM, Latip AFA, Suah FBM
    Int J Biol Macromol, 2025 Mar;296:139787.
    PMID: 39809393 DOI: 10.1016/j.ijbiomac.2025.139787
    Antibiotics are emerging environmental contaminants posing critical health risks due to their tendency to concentrate in living things and eventually infiltrate the human body. Sulfamethoxazole (SMZ) is among the commonly detected antibiotics in wastewater requiring effective removal approach. A sustainable, thermally stable and easily separable magnetic sporopollenin-cellulose triacetate (Msp-CTA) was developed via a simple step synthesis for eliminating SMZ from aqueous solution. The assessment of Msp-CTA characteristics via instrumentations revealed a mesoporous adsorbent having COO-, -OH, CO, and Fe - O as its potential adsorption binding sites. Improved stability gained from blending sporopollenin (Sp) and cellulose triacetate (CTA) was affirmed through TGA analysis. Minimal competition for the adsorption sites by H+ and OH- favours efficient SMZ adsorption onto Msp-CTA at pH 3 and 5 with %removal of 78.7 and 83.1 %, respectively, using 40 mg/L initial SMZ concentration. The monolayer adsorption capacities 15.14 and 15.52 mg/g were obtained from nonlinear and linear Langmuir isotherms, respectively. The adsorption process is best described by Temkin > Hill > Langmuir > Freundlich isotherms based on proximity of correlation coefficient R2 to 1. The best fit to Temkin suggests that SMZ adsorption's energy decreases proportionally with increasing surface coverage of Msp-CTA. The adsorption process is arbitrated to conform best to pseudo-second order (PSO) kinetic, though a bias is perceived in the linear fitting. Msp-CTA shows reusable potential with 65.9 % desorption at third cycle. The thermodynamic studies revealed an endothermic process. Hence, Msp-CTA demonstrates potential as an alternative adsorbent for sulfonamide-based antibiotics' removal from wastewater.
    MeSH terms: Adsorption; Biopolymers/chemistry; Carotenoids; Hydrogen-Ion Concentration; Kinetics; Water/chemistry; Water Purification/methods; Waste Water/chemistry
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