Browse publications by year: 2025

  1. Lee WS, Chew KS, Huang JG, Tanpowpong P, Mercado KSC, Reodica A, et al.
    Front Pediatr, 2025;13:1487253.
    PMID: 40051907 DOI: 10.3389/fped.2025.1487253
    BACKGROUND: There is a paucity of knowledge on disease phenotype and outcome of very early-onset (VEO) inflammatory bowel disease (VEO-IBD) from recently developed and developing countries, including from Southeast Asia. We studied disease phenotype, clinical characteristics, management and outcome of VEO-IBD in South and Southeast Asian children.

    MATERIALS AND METHODS: We extracted data from a multicentre Asian pediatric (onset <18 years) IBD registry. VEO- and later-onset pediatric (LO-p) IBD were defined as onset of disease <6 years and ≥6 years, respectively. We excluded monogenic IBD.

    RESULTS: Of 440 children with IBD cases; 112 (25.5%) were VEO-IBD; Crohn's disease (CD) 36 (32.1%); ulcerative colitis (UC) 68 (60.7%), and IBD-unspecified 7 (7.1%). UC was more common in VEO-IBD while CD more common in LO-pIBD (CD = 68.9% vs. UC = 25.9%; p 

  2. Said A, Said FM, Nambiar N
    Iran J Nurs Midwifery Res, 2025;30(1):18-24.
    PMID: 40052035 DOI: 10.4103/ijnmr.ijnmr_461_21
    BACKGROUND: In Indonesia, several hospitals have designed various forms of discharge planning in line with the guidelines provided by the Indonesian Endocrinology Association. These initiatives were implemented to enhance the quality of healthcare service. Despite the efforts made by the government, the rate of non-compliance (9%) and readmission (20%) has continued to increase. This indicates that there is a need to reevaluate the existing discharge planning module. Therefore, this study aims to validate the contents of the diabetes mellitus discharge planning module, which was incorporated into the summary of diabetes self-care activity. The effectiveness of the intervention in improving the compliance of patients with self-care activity was also evaluated.

    MATERIALS AND METHODS: This study used a mixed methodological approach, which combined an evaluation method and an experimental quantitative design. The content validity of the module used was carried out using professional judgment, involving competent experts in the bahteramas hospital in southeast Sulawesi, Indonesia. The sample population consisted of sixty-five randomly selected respondents, who participated in the validation of the summary of Diabetes Self-Care Activities (SDSCA) questionnaire, which had three phases, namely pre-test, during, fieldwork, and post-test.

    RESULTS: The content validity of the module was tested using Aiken's v, and the results ranged from 0.82 to 0.88 for each session, indicating that it was valid. Furthermore, the internal consistency (Cronbach's alpha) obtained in this study was acceptable, with values ranging from 0.60 to 0.92.

    CONCLUSIONS: The results showed that the diabetes mellitus discharge planning module could be used to improve self-care among patients.

  3. Yang Q, Bee YM, Lim CC, Sabanayagam C, Yim-Lui Cheung C, Wong TY, et al.
    EClinicalMedicine, 2025 Mar;81:103089.
    PMID: 40052065 DOI: 10.1016/j.eclinm.2025.103089
    BACKGROUND: Artificial Intelligence (AI) has been used to automate detection of retinal diseases from retinal images with great success, in particular for screening for diabetic retinopathy, a major complication of diabetes. Since persons with diabetes routinely receive retinal imaging to evaluate their diabetic retinopathy status, AI-based retinal imaging may have potential to be used as an opportunistic comprehensive screening for multiple systemic micro- and macro-vascular complications of diabetes.

    METHODS: We conducted a qualitative systematic review on published literature using AI on retina images to detect systemic diabetes complications. We searched three main databases: PubMed, Google Scholar, and Web of Science (January 1, 2000, to October 1, 2024). Research that used AI to evaluate the associations between retinal images and diabetes-associated complications, or research involving diabetes patients with retinal imaging and AI systems were included. Our primary focus was on articles related to AI, retinal images, and diabetes-associated complications. We evaluated each study for the robustness of the studies by development of the AI algorithm, size and quality of the training dataset, internal validation and external testing, and the performance. Quality assessments were employed to ensure the inclusion of high-quality studies, and data extraction was conducted systematically to gather pertinent information for analysis. This study has been registered on PROSPERO under the registration ID CRD42023493512.

    FINDINGS: From a total of 337 abstracts, 38 studies were included. These studies covered a range of topics related to prediction of diabetes from pre-diabetes or non-diabeticindividuals (n = 4), diabetes related systemic risk factors (n = 10), detection of microvascular complications (n = 8) and detection of macrovascular complications (n = 17). Most studies (n = 32) utilized color fundus photographs (CFP) as retinal image modality, while others employed optical coherence tomography (OCT) (n = 6). The performance of the AI systems varied, with an AUC ranging from 0.676 to 0.971 in prediction or identification of different complications. Study designs included cross-sectional and cohort studies with sample sizes ranging from 100 to over 100,000 participants. Risk of bias was evaluated by using the Newcastle-Ottawa Scale and AXIS, with most studies scoring as low to moderate risk.

    INTERPRETATION: Our review highlights the potential for the use of AI algorithms applied to retina images, particularly CFP, to screen, predict, or diagnose the various microvascular and macrovascular complications of diabetes. However, we identified few studies with longitudinal data and a paucity of randomized control trials, reflecting a gap between the development of AI algorithms and real-world implementation and translational studies.

    FUNDING: Dr. Gavin Siew Wei TAN is supported by: 1. DYNAMO: Diabetes studY on Nephropathy And other Microvascular cOmplications II supported by National Medical Research Council (MOH-001327-03): data collection, analysis, trial design 2. Prognositc significance of novel multimodal imaging markers for diabetic retinopathy: towards improving the staging for diabetic retinopathy supported by NMRC Clinician Scientist Award (CSA)-Investigator (INV) (MOH-001047-00).

  4. Kong AS, Lim SE, Cheng WH, Yuswan MH, Tan NP, Lai KS
    Pol J Microbiol, 2025 Mar 01;74(1):1-18.
    PMID: 40052212 DOI: 10.33073/pjm-2025-010
    Antimicrobial resistance (AMR) poses a formidable challenge in global healthcare, driving the exploration of natural products for novel antimicrobials. Among these, essential oils (EOs) derived from medicinal plants are rich sources of diverse bioactive compounds. Monoterpenes and monoterpenoids, critical constituents of EOs, have emerged as promising agents in combating multidrugresistant (MDR) pathogens. This review analyzed recent literature on the efficacy of monoterpenes against AMR, highlighting their broad-spectrum activity and potential as alternative therapeutic options for MDR infections. Mechanistic insights reveal their ability to disrupt cell membranes, inhibit biofilm formation, and modulate gene expression linked to virulence and resistance, thereby reducing microbial viability through alterations in membrane potential, enzymatic activity, and genetic regulation. Synergistic interactions between monoterpenes and conventional antibiotics are also elucidated. Innovative approaches in monoterpene research are explored, although challenges such as resistance, limited solubility, volatility, and potential toxicity are acknowledged, emphasizing the need for advanced formulation strategies and interdisciplinary research. The synergy observed with conventional antibiotics, coupled with their ability to target specific microbial resistance mechanisms, underscores the potential of monoterpenes in combating antibioticresistant infections. Future investigations should prioritize optimizing monoterpenes' therapeutic properties and assessing their safety profiles to fully exploit their potential in addressing AMR.
    MeSH terms: Bacteria/drug effects; Drug Synergism; Humans; Microbial Sensitivity Tests; Biofilms/drug effects; Drug Resistance, Multiple, Bacterial
  5. Tee HS, Liang J, Aziz NA, Zhou X, Hisham HA, Tan KE, et al.
    J Med Virol, 2025 Mar;97(3):e70269.
    PMID: 40042148 DOI: 10.1002/jmv.70269
    Epstein-Barr virus (EBV) is associated with cancers, including lymphomas and nasopharyngeal carcinoma (NPC). To date, risk variants for NPC were mainly identified from Chinese populations, which dominated the world's total number of cases. Although Southeast Asia (SEA) countries have among the world's top yet intriguingly diverse NPC age-standardized incidence rates across subpopulations, data on EBV from SEA remains scarce. In this study, we examined 83 NPC patients of different ancestries for the presence of risk haplotypes associated with the Southern Chinese NPC and generated and analyzed 67 EBV sequences (from tissue, patient-derived xenografts and lymphoblastoid cell lines of 60 NPC patients) together with 838 published EBV genomes. Our study revealed that NPC patients of non-Chinese ancestry had fewer risk variants and haplotypes that are associated with Southern Chinese NPC and clustered distinctly from lymphomas, Southern Chinese NPC, and non-cancer controls. The distribution of non-synonymous variants was similar among NPC patients of Chinese ancestry, irrespective of geographical location. Meanwhile, non-synonymous variants in genes related to packaging, latency, and structural proteins such as BPLF1, LF3, and LMP1 varied across different ancestries. Our findings suggest possibilities of EBV adaptation to host genetics for NPC pathogenesis and warrant further research for the understudied NPC subpopulations.
    MeSH terms: Adult; Aged; Asia, Southeastern/epidemiology; Carcinoma/genetics; Carcinoma/virology; Female; Haplotypes; Humans; Male; Middle Aged; Phylogeny; Genetic Variation*; Asian Continental Ancestry Group/genetics
  6. Chakravarthi KK, Nelluri V, Sugavasi R, Reghunadhan D
    Ann Afr Med, 2025 Mar 05.
    PMID: 40042524 DOI: 10.4103/aam.aam_291_24
    BACKGROUND: Atlanto-occipital and atlantoaxial assimilation are rare congenital abnormalities characterized by abnormal fusion of the atlas vertebra with the occipital bone or the axis vertebra, respectively, within the craniovertebral junction. These anomalies can significantly alter the biomechanics and structural integrity of the cervical spine, leading to diverse clinical presentations ranging from restricted neck mobility to neurological deficits. Accordingly, this study aims to investigate the prevalence, anatomical abnormalities, and clinical significance of atlanto-occipital and atlantoaxial assimilation in the Asian population.

    MATERIALS AND METHODS: A total of 82 human dry skulls and 82 sets of dry cervical vertebrae were examined for the presence of atlanto-occipital and atlantoaxial assimilation. Detailed morphometric analysis was performed to document the fusion patterns and variations in anatomical structures. The data were analyzed to determine the prevalence of these congenital anomalies and their implications for craniovertebral stability and neurological function.

    RESULTS: Among the 82 skulls and 82 cervical vertebrae examined, atlanto-occipital assimilation was identified in 3 skulls (3.65%), whereas atlantoaxial assimilation was observed in 2 cases (2.43%). Combined occurrences of both anomalies were found in 2 skulls (2.43%) of the sample.

    CONCLUSION: A thorough understanding of atlanto-occipital and atlantoaxial assimilation is clinically crucial due to its potential impact on the structural and functional dynamics of the craniovertebral junction. These congenital anomalies, though infrequent, can pose considerable clinical challenges, including compromised craniovertebral stability and an increased risk of neurological symptoms. Early diagnosis and careful clinical evaluation are crucial for managing potential complications associated with these conditions.

  7. Sivalingam S, Haranal MY, See WS
    Pediatr Cardiol, 2025 Mar 05.
    PMID: 40042536 DOI: 10.1007/s00246-025-03808-6
    Aortic valve reconstruction in the pediatric population presents significant surgical challenges as the ideal repair requires an individualized approach considering various factors. This study aims to analyze the factors influencing outcomes of aortic valve neocuspidization (AV Neo) in pediatric patients. A retrospective cohort study involving pediatric patients who underwent neocuspidization (congenital and acquired) between 2016 and 2023. Clinical outcomes assessed included mortality, reintervention, structural valve degeneration and infective endocarditis. Echocardiographic measurements included peak and mean gradient, indexed vena contracta jet width, annular diameter, and coaptation height. Freedom from events was analyzed using Kaplan-Meier analysis. Outcomes were compared based on congenital or acquired valve lesions. AV neo was performed in 53 patients with a mean age of 10.7 ± 3.91 years, of which 29 had congenital aortic valve disease. There was one early in-hospital mortality. At discharge, the mean coaptation height was 9.9 ± 1.0 mm, the peak gradient was 12.0 mmHg, and less than mild regurgitation was observed. The median follow up period was 27.4 months. Forty-four patients (83%) remained event-free. A Significant aortic annular growth was noted, with rheumatic etiology patients showed the most stable coaptation height over time. Truncus arteriosus patients demonstrated rapid growth of the annulus leading to accelerated truncal valve regurgitation progression. AV Neo is feasible with acceptable short-term results in pediatric patients with congenital and acquired etiology. The procedure allows significant physiological annular growth, particularly benefiting patients with rheumatic aortic valve disease, but less favorable among patients with truncal valve pathology.
  8. Yazid SNE, Selamat J, Ismail SI, Sanny M, Samsudin NIP
    J Appl Microbiol, 2025 Mar 03;136(3).
    PMID: 40042983 DOI: 10.1093/jambio/lxaf049
    AIM: The present work investigated the efficacy of native biocontrol candidates (antagonists) against aflatoxins, ochratoxin A (OTA), and fumonisins produced by native mycotoxigenic fungi isolated from Malaysia at different pathogen: antagonist inoculum ratios on grain corn agar and grain corn kernels.

    METHODS AND RESULTS: Five pathogen: antagonist inoculum ratios (100:0, 75:25, 50:50, 25:75, and 0:100) were employed. Non-aflatoxigenic Aspergillus flavus Af1KD and Af5TD, and Penicillium janthinellum were used against aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) by A. flavus. Against OTA by A. niger, and fumonisin B1 (FB1) and fumonisin B2 (FB2) by Fusarium verticillioides and F. proliferatum, respectively, the antagonists Trichoderma asperelloides, T. asperellum, and T. harzianum were used. Non-aflatoxigenic A. flavus Af1KD was the most effective against AFB1 and AFB2 at all tested ratios and substrates. All Trichoderma spp. were effective against OTA by A. niger on grain corn agar at all tested ratios. Trichoderma asperelloides and T. asperellum were effective against FB1 and FB2 produced by F. verticillioides at all tested ratios and substrates. Trichoderma asperelloides was effective against FB1 and FB2 by F. proliferatum at all tested ratios and substrates.

    CONCLUSION: The native biocontrol candidates were effective against mycotoxigenic fungi and mycotoxin production on grain corn agar and grain corn kernels, and could be developed into biocontrol agents.

    MeSH terms: Aflatoxins*; Agar; Aspergillus niger/growth & development; Aspergillus niger/metabolism; Hypocreales; Malaysia; Ochratoxins*; Aflatoxin B1/metabolism; Biological Control Agents/pharmacology
  9. Wan AJ, Foo JB, Loo JSE
    J Biomol Struct Dyn, 2025 Mar 05.
    PMID: 40043005 DOI: 10.1080/07391102.2025.2475223
    Designing small molecule inhibitors that are highly selective for Golgi alpha mannosidase II (GMII) over lysosomal alpha mannosidase (LM) remains crucial for the development of novel anticancer drugs targeting the N-glycosylation pathway. Studies have previously identified an unconserved 'anchor site' in GMII that represents an attractive target for achieving selectivity. In this study we conduct molecular dynamics simulations and free energy calculations of GMII and LM with their natural oligosaccharide substrates to investigate the potential of the anchor site. Our findings reveal that the corresponding N-acetylglucosamine residue remains tightly bound to the anchor site in GMII, helping stabilize overall substrate binding to GMII, while the lack of a similar conserved site in LM allows the substrate to fluctuate more freely. Our simulations also suggest that besides stabilizing the catalytic site mannose for cleavage, the anchor site residue may play a role in facilitating the release of the holding site mannose and its transition to the catalytic site in GMII. Subsequently, free energy calculations reveal that the anchor site N-acetylglucosamine contributes 4.107 kcal/mol to the free energy of binding in GMII, with a significantly smaller contribution of 1.035 kcal/mol in LM. This difference of 3.072 kcal/mol in the free energy of binding represents potential gains in selectivity that could be achieved by targeting the anchor site in GMII. Taken together, these findings provide further evidence on the potential of targeting the anchor site in the design of highly selective GMII inhibitors.
  10. Mamat Yusof MN, Chew KT, Kampan NC, Hafizz AMHA, Shafiee MN
    PLoS One, 2025;20(3):e0312765.
    PMID: 40043041 DOI: 10.1371/journal.pone.0312765
    Checkpoint programmed death-1 (PD-1) and programmed cell death ligands (PD-Ls) are negative immunoregulatory molecules that assist tumour cells in evading the immune system. The interaction of PD-1 and PD-Ls inhibits T cells and tumour-infiltrating lymphocytes (TILs) while increasing the function of immunosuppressive regulatory T cells (Tregs). This leads to the evasion of the immune response by tumour cells. The roles of PD-1, PD-L1, and PD-L2 in endometrial cancer (EC) have not been fully elucidated. This study investigates the mRNA gene expression and soluble protein levels of these molecules in EC compared to controls, with detailed analysis of clinical profiles. The results showed that EC had significantly higher mRNA gene expression and soluble protein levels of PD-L1 and PD-L2, but not PD-1. Specifically, PD-1 mRNA gene expression was significantly higher in cases with less than 50% myometrial invasion. Additionally, the soluble protein level of PD-1 was substantially higher in patients under the age of 60. Higher gene expression of PD-L1 was observed only in advanced stages of EC. However, the soluble PD-L1 protein level was significantly elevated in type II EC, advanced stage, higher grade, lympho-vascular space invasion (LVSI), and in cases with myometrial invasion of 50% or more. PD-L2 mRNA gene expression and soluble protein levels significantly differed across all clinical profiles except for LVSI. These findings suggest that PD-1, PD-L1, and PD-L2 may serve as potential predictive biomarkers, which could be beneficial for the management of endometrial cancer patients through immunotherapy.
    MeSH terms: Adult; Aged; Female; Humans; Middle Aged; RNA, Messenger/genetics; RNA, Messenger/metabolism; Gene Expression Regulation, Neoplastic
  11. Song JY, Liu M, Zhang M, Sulaiman Z, Ismail TAT, Li SM, et al.
    Cancer Nurs, 2025 Mar 04.
    PMID: 40043196 DOI: 10.1097/NCC.0000000000001476
    BACKGROUND: Improving family function and resilience among cancer caregivers is a vital clinical concern requiring thorough research. Psychosocial interventions are expected to enhance family function and strengthen resilience; however, the outcomes can differ significantly, highlighting the necessity for further investigation.

    OBJECTIVES: This study aimed to evaluate the comparative effectiveness of psychosocial interventions in enhancing family function and resilience among caregivers of cancer patients based on data derived from randomized controlled trials.

    METHODS: A systematic review was performed by searching 6 electronic databases and clinical trial registries until July 12, 2024. Pairwise and network meta-analyses (NMA) were performed using the network package in STATA 16.

    RESULTS: This review identified 12 studies with a total of 1162 participants. The pairwise meta-analysis revealed a significant positive impact of psychosocial interventions on family function: standardized mean difference (SMD), 1.34; 95% confidence interval [CI], 0.16-2.52. NMA showed family interaction approaches (SMD, 0.69; 95% CI, 0.22-1.15) and psychoeducation interventions (SMD, 0.66; 95% CI, 0.24-1.08) both led to improvements in family function. However, neither the pairwise meta-analysis nor the NMA revealed the positive effects of psychosocial interventions on resilience.

    CONCLUSION: Evidence suggests that psychosocial interventions can moderately improve family function. Among the various interventions explored, the family interactions demonstrated the most significant potential for effectiveness. However, resilience meta-analyses have not supported our psychosocial intervention recommendations.

    IMPLICATIONS FOR PRACTICE: This study offers valuable insights for nursing practice, emphasizing the need for nurses to consider the relationships and resources available within the families of cancer patients while providing care.

  12. Looi JC, Allison S, Bastiampillai T, Kisely S, Maguire PA, Woon L, et al.
    Aust J Gen Pract, 2025 Mar;54(3):91-94.
    PMID: 40043283 DOI: 10.31128/AJGP-09-23-6967
    BACKGROUND: Recently, there has been media and public interest regarding discontinuation of antidepressant treatment, especially in primary care. In this context, we provide a primary care update on the maintenance or discontinuation of antidepressant medication for the treatment of moderate-to-severe depression in adults.

    OBJECTIVE: This article aims to provide a primary care update on the maintenance or discontinuation of antidepressant medication for the treatment of moderate-to-severe depression in adults. To this end, we performed a qualitative narrative review and provide commentary on recent research and systematic reviews.

    DISCUSSION: In primary care, recent research has shown that there are substantial risks of depressive relapse resulting from antidepressant discontinuation. For a first episode of moderate-to-severe depression, antidepressants should be continued for 9-12 months after remission. Systematic reviews indicate that, overall, there is limited evidence of benefits from ceasing antidepressant treatment for recurrent depression. The existing evidence base on antidepressant withdrawal is limited in quality and extent, providing some evidence of harms, such as relapse, and not necessarily any gains, although reducing the burden of adverse effects is a consideration. There is a benefit-to-risk ratio in any decision to continue or withdraw antidepressant treatment.

    MeSH terms: Humans; Primary Health Care; Withholding Treatment
  13. Baharudin AS, Zainol N, Aziz NH
    J Environ Manage, 2025 Apr;378:124734.
    PMID: 40043568 DOI: 10.1016/j.jenvman.2025.124734
    The mushroom production industry generates a significant amount of expired mushroom blocks (EMBs), posing economic and environmental challenges. To address this issue, biotechnology research has focused on utilizing EMBs to produce biofertilizers rich in nitrogen and phosphorus. Therefore, this study aimed to evaluate the significant parameters influencing biofertilizer production from mushroom waste through design of experiment approach. Five factors were investigated: mushroom waste (MW) content (70% MW with 30% Bokashi Bran and 30% MW with 70% Bokashi Bran), Bokashi fermentation duration (5-10 days), agitation (presence or absence), waste size (cut or uncut), and drying temperature (60 °C-105 °C). A two-level factorial analysis was used to identify the most influential factors affecting biofertilizer production with high nitrogen and phosphorus content. Both nutrients were measured using a HACH Spectrophotometer. Based on the results, the optimal conditions for high nitrogen content included a drying temperature of 104 °C, 70% mushroom waste content, uncut waste size, and 10 days of fermentation with agitation, yielding 1.62 g/L nitrogen. The optimal conditions for achieving maximum phosphorus content were a drying temperature of 60 °C, 70% mushroom waste content, cut waste, and a 10-day fermentation period without agitation, resulting in a phosphorus concentration of 3.39 g/L. Overall, the best conditions for both nitrogen and phosphorus content were a drying temperature of 80 °C, 70% mushroom waste content, cut waste size, and 10 days of fermentation, with nitrogen and phosphorus contents of 1.10 g/L and 2.61 g/L, respectively. The identifed optimal conditions for producing biofertilizers with high nitrogen and phosphorus content from mushroom waste could offer a sustainable solution to both agricultural and environmental challenges.
    MeSH terms: Agaricales*; Fermentation*; Phosphorus*; Temperature
  14. Mao Y, Zheng Y, Xie R, Tan CP, Wang Y, Zhang Z
    Food Chem, 2025 Jun 15;477:143566.
    PMID: 40043605 DOI: 10.1016/j.foodchem.2025.143566
    Diacylglycerol (DAG), a functional lipid with two isomers, exhibits distinct physical properties that affect oil systems. This study investigated the crystallization behaviors and physical property stabilities in binary systems composed of sn-1,3 DAG and its migration equilibrium product (ME-DAG, with an sn-1,3 DAG:sn-1,2 DAG ratio of 65:35) at mass ratios of 20 %, 50 %, and 80 % with palm kernel oil (PKO). The results revealed that the sn-1,3 DAG/PKO systems exhibited faster crystallization rates and more pronounced eutectic effects than the ME-DAG/PKO systems. In addition, sn-1,3 DAG inhibited the polymorphic transformation (α → β' → β) in the DAG/PKO binary systems. Higher DAG concentrations enhanced the crystallization and nucleation rates. Furthermore, sn-1,3 DAG at 50 % and 80 % notably reduced post-hardening and moderated the slope of the solid fat content curve. Microscopic analysis revealed the presence of Maltese cross crystals and disordered acicular crystals in the DAG/PKO binary systems. These findings underscore the critical role of DAG acyl migration in compatibility, crystallization kinetics, polymorphism, and texture, offering insights for developing functional plastic lipids with tailored properties.
    MeSH terms: Crystallization*; Kinetics
  15. Stewart LB, Escolar EL, Philpott J, Cox-Singh J, Singh B, Conway DJ
    Int J Parasitol, 2025 Mar 04.
    PMID: 40043895 DOI: 10.1016/j.ijpara.2025.02.003
    Replication rates and virulence of pathogens are hypothesised to evolve in response to varying intensity of transmission and competition among genotypes. Under exponential growth conditions in culture, clinical isolates of the malaria parasite Plasmodium falciparum have variable intrinsic multiplication rates, but comparisons of samples from different areas are needed. To analyse parasites from an area of low endemicity, Malaysian clinical isolates cryopreserved prior to malaria elimination were studied. The mean and range of P. falciparum multiplication rates in Malaysian isolates were no less than that seen among isolates from more highly endemic populations in Africa, which does not support a hypothesis of adaptation to prevailing levels of infection endemicity. Moreover, the distribution of multiplication rates was similar between isolates with single parasite genotypes and those containing multiple genotypes, which does not support a hypothesis of facultative adjustment to competing parasites. Based solely on clinical isolates, the findings indicate that parasites may not evolve lower multiplication rates under conditions of reduced transmission, and that the virulence potential is likely to be undiminished in pre-elimination settings. This encourages efforts to eliminate endemic infection completely, as has been achieved at the national level in Malaysia.
  16. Huang Z, Zhong J, Zhang G, Chai CA, Ai G, Zeng G, et al.
    Urology, 2025 Mar 06.
    PMID: 40043958 DOI: 10.1016/j.urology.2025.02.042
    OBJECTIVE: To investigate the changes in the stone composition between 2 stages of stone surgery.

    METHODS: We prospectively enrolled 600 patients from a single medical center between October 2015 and June 2021. The stones were analyzed using Fourier transformation infrared spectrometer (Bruker, Karlsruhe, Germany). Calculi were classified based on their primary component. Ultimately, a total of 520 patients met the criteria and entered the final analyses. The stone composition between the 2 stages of surgery was compared.

    RESULTS: Of the 520 patients, 277 were male and the mean age was 52.9±12.8 years. The average time delay between 2 stone analyses was approximately 33.2 days. One hundred and eighty-six cases (35.8%) had different compositions on the second-stage analyses, with a total of 67 (36.0%) cases transitioning to a totally different type of stones. Forty-one (16.5%) cases initially identified as infection stones changed to non-infection stones and 35 (12.9%) cases transformed from non-infection stones to infected stones.

    CONCLUSION: Up to 35.8% of stones underwent changes during the second stone composition analysis, and up to 36.0% of these altered stones had transformed to a totally different type. Conducting stone composition analysis between 2 stages of surgery is essential for the development of follow-up treatment and drug prevention plans.

  17. Adiiba SH, Chan ES, Ng WZ, Song CP
    Int J Biol Macromol, 2025 Mar 06;306(Pt 4):141650.
    PMID: 40044000 DOI: 10.1016/j.ijbiomac.2025.141650
    Several lipases have been used to catalyze the hydrolysis of crude palm oil (CPO) to concentrate palm phytonutrients. Previously, the retention of phytonutrients during hydrolysis has been improved with a small addition of ethylenediaminetetraacetic acid. However, the production of free fatty acids (FFA) via enzymatic hydrolysis has yet to be optimised. Thus, for the first time, this study explored the combined use of AY 30SD (from Candida rugosa) and Eversa® Transform 2.0, ET 2.0 (from Aspergillus oryzae) to investigate the effect of reaction time and temperature on the lipases' performance towards the production of FFA during the alcohol-free process of producing phytonutrients concentrates. The results revealed that the application of combi-lipases at 45 °C yielded the highest final FFA content due to ET 2.0's affinity towards longer fatty acid chain lengths and AY 30SD's effectiveness in hydrolysing TAG molecules. With total enzyme concentration of 0.2 wt%, incorporating combi-lipase AY 30SD and ET 2.0 produced >95 wt% of FFA. Furthermore, a basic economic analysis validated that the combination of 0.15 wt% AY 30SD and 0.05 wt% ET 2.0 reduced the reaction time by seven times (completed within 24 h), potentially leading to a substantial increase in profit without sacrificing the phytonutrients retention.
  18. Baek SJ, Lim H, Oh S, Lee S, Kim Y, Song JE, et al.
    Dis Colon Rectum, 2025 Mar 05.
    PMID: 40044408 DOI: 10.1097/DCR.0000000000003706
    BACKGROUND: Colorectal cancer is lacking non-invasive and efficient diagnostic markers. Recently, volatile organic compounds present in exhaled breath have emerged as potential diagnostic indicators for various diseases.

    OBJECTIVE: This study aimed to establish a predictive model including exhaled volatile organic compounds for the diagnosis of colorectal cancer.

    DESIGN: Prospective comparative study.

    SETTINGS: Single academic medical center.

    PATIENTS: Exhaled breath was collected prospectively from 112 colorectal cancer patients and 51 healthy controls. The exhaled breath of the research participants was collected under the control of various conditions, such as eating or smoking prior to collection.

    INTERVENTIONS: A total of 53 volatile organic compounds was analyzed in exhaled breath using thermal desorption-gas chromatography/mass spectrometry.

    MAIN OUTCOME MEASURES: After correcting various clinical variables that may affect volatile organic compound, the concentrations detected in the colorectal cancer group and the healthy control group were compared.

    RESULTS: Four predictive models were established using both matched clinical variables and volatile organic compound values to differentiate between colorectal cancer and healthy controls. In the four models, clinical variables of age, gender, room temperature, heating and cooling, fasting duration, intake of grilled meat or fish, and bowel preparation were included, along with levels of eight volatile organic compounds (ethanol, decanal, 1-iodononane, dodecane, hexanal, 1-nonene, (1S)-(-)-alpha-pinene, and acetophenone). The sensitivity of the four prediction models ranged from 84.0% to 98.2%, the specificity ranged from 89.2% to 93.8%, and the area under the receiver operating characteristic curve ranged from 92.1% to 98.3%.

    LIMITATIONS: The demographics of the participants in the groups were different.

    CONCLUSIONS: Eight types of volatile organic compounds in the exhaled breath of the colorectal cancer group were significantly different from those of the healthy control group. Our results support the diagnosis of colorectal cancer using a predictive model combining volatile organic compounds and specific clinical variables. See Video Abstract.

  19. Ramkumar MS, Subramani J, Sivaramkrishnan M, Munimathan A, Michael GKO, Alam MM
    Sci Rep, 2025 Mar 05;15(1):7755.
    PMID: 40044727 DOI: 10.1038/s41598-025-90062-8
    Research in industrial grid energy management is essential due to increasing energy demands, rising costs, and the integration of renewable sources. Efficient energy management can reduce operational costs, enhance grid stability, and optimize resource allocation. Addressing these challenges requires advanced techniques to balance supply, demand, and storage in dynamic industrial settings. In this study, a new hybrid algorithm is used for system modelling and low-cost, optimal management of Micro Grid (MG) networked systems. Optimizing micro-sources to reduce electricity production costs through hourly, day-ahead, and real-time scheduling was the process' primary goal.This research proposes a Quadratic Interpolation and New Local Search for Greylag Goose Optimisation (QI-NLS-G2O) and Gaussian Radius Zone Perceptron Net (GRZPNet) technique based energy management scheme for Multi-Energy Microgrids (MEMG) to help the Energy Management System (EMS) formulate optimal dispatching strategies under Renewable Energy Source (RES) uncertainty. To precisely represent the MEMG's operational state, the scheduling process incorporates an off-design performance model for energy conversion devices. Utilising MG inputs such as Wind Turbines (WT), Photovoltaic arrays (PV), and battery storage with associated cost functions, the GRZPNet learning phase based on QI-NLS-G2O is utilised to forecast load demand. The QI-NLS-G2O optimises the MG configuration according to the load demand. The MATLAB/Simulink working platform is used to implement the suggested hybrid technique, which is then contrasted with alternative approaches to solving problems.The proposed model significantly improves dispatching accuracy, reducing RES uncertainty impacts by approximately 15% and enhancing MEMG performance efficiency by up to 20% in simulations.
  20. Mokmin NAM, Rassy RP, Yie DL
    Sci Rep, 2025 Mar 05;15(1):7682.
    PMID: 40044728 DOI: 10.1038/s41598-025-92533-4
    Students with dyslexia (SWDs), which is a specific learning problem that impairs reading, often experience inadequate academic performance and have a negative perception of themselves in general. However, despite the recognized benefits of Physical Education (PE) classes for physical and psychological development towards SWDs, barriers such as fear of injury, limited resources, and a shortage of trained teachers undermine the inclusivity of PE programs. The present study aims to uncover the issues when teaching PE using the traditional approach and investigate the possible opportunities, challenges, and effectiveness of integrating augmented reality (AR) technology in PE classes. SWDs (n = 22) and PE teachers (n = 2) were selected using purposive sampling, and interview sessions were conducted before and after the intervention. The transcription of the recorded sessions was then analyzed using thematic analysis, and a thematic map was constructed based on the findings. The implementation of AR in PE instruction has the potential to create a more inclusive learning environment, breaking down barriers and promoting the overall well-being of SWDs in Malaysian schools.
    MeSH terms: Adolescent; Adult; Child; Female; Humans; Malaysia; Male; School Teachers/psychology
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