A voltammetric immunosensor based on a nanoporous alumina millirod for detection of porcine serum albumin

Mahmud AH 1 , Salahuddin NM 1 , Md Jani AM 1 , Abu Bakar NF 2 , Zainal Abidin SAS 3 , Mohd Zain Z 4 Show all authors , Low KF 5

Affiliations 

  • 1 Faculty of Applied Sciences, Universiti Teknologi MARA, Tapah Campus, Tapah Road, Perak 35400 Malaysia
  • 2 School of Chemical Engineering, College of Engineering, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia
  • 3 Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia; Malaysia Institute of Transport, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia
  • 4 Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia; Electrochemical Material and Sensors (EmaS) Group, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia
  • 5 Faculty of Applied Sciences, Universiti Teknologi MARA, Tapah Campus, Tapah Road, Perak 35400 Malaysia; Electrochemical Material and Sensors (EmaS) Group, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam, Selangor 40450 Malaysia. Electronic address: lowkimfatt@uitm.edu.my
Food Chem, 2023 Jun 15;411:135493.
PMID: 36689871 DOI: 10.1016/j.foodchem.2023.135493

Abstract

A voltammetric immunosensor was developed for detection of porcine serum albumin (PSA) to identify raw meat products adulterated with pork. A novel strategy to fabricate multiple individual nanoporous alumina (NPA) millirods (length, 5.0 mm; diameter, 1.0 mm) as the biorecognition platform is described. Each NPA millirod was covalently bioconjugated with anti-PSA capturing antibodies (α-PSAC). Following immunocapture, the PSA bound to the α-PSAC/NPA millirod bioconjugate were tagged with gold nanoparticles (AuNPs) functionalized with anti-PSA detection antibodies as the signaling probe. Subsequently, the AuNPs were voltammetrically analyzed to quantify the target PSA. The immunosensor exhibited 100 % specificity and high sensitivity to PSA with a limit of detection (LoD) of 50 (range, 0-1000) pg/mL (R2 = 0.9907). Real-world applicability was successfully validated using pork/beef adulterated mixtures with a LoD of 0.05 % (w/w). Overall, the detection performance of the proposed immunosensor was excellent and, thus, is suitable for surveillance of food safety and quality.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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