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Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats

Ibrahim MY 1 , Hashim NM 1 , Dhiyaaldeen SM 2 , Al-Obaidi MM 3 , El-Ferjani RM 4 , Adam H 5 Show all authors , Alkotaini B 6 , Batran RA 3 , Ali HM 7

Affiliations 

  • 1 Department of Pharmacy, Faculty of Medicine, University of Malaya 50603 Kuala Lumpur, Malaysia
  • 2 Department of Microbiology, Faculty of medicine, University of Duhok, 78 Kurdistan, Iraq
  • 3 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 4 Department of Chemistry, Faculty of Science, University of Benghazi, 1308 Benghazi, Libya
  • 5 School of Pharmacy, Ahfad University for Women (AUW), 167 Omdurman, Sudan
  • 6 Department of Chemical Engineering, Faculty of Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea
  • 7 Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
Sci Rep, 2016 05 27;6:26819.
PMID: 27229938 DOI: 10.1038/srep26819

Abstract

Manganese is a crucial element for health. In this study, the gastroprotective efficacy of Mn (II) complex (MDLA) against acidified ethanol (HCl/Ethanol)-induced gastric ulceration in rats was evaluated. The animals were distributed into 5 groups. Groups 1 and 2 received carboxymethylcellulose (CMC), group 3 was pretreated with omeprazole, and groups 4 and 5 were given 10 and 20 mg/kg of MDLA, respectively. After one hour, CMC and HCl/Ethanol were given to groups 2-5 whilst the animals in group 1 were ingested with CMC. After sacrifice, gastric lesions were evaluated by wall mucus, gross appearance, histology, antioxidant enzymes and immunohistochemistry. Group 2 displayed severe gastric damage with a significant reduction in wall mucus. Conversely, gastric lesions were reduced in groups 3-5 by 85.72%, 56.51% and 65.93%, respectively. The rats in groups 3-5 showed up-regulation of heat shock protein 70 (Hsp70) with down-regulation of Bcl-2-associated protein x (Bax). Pretreatment with omeprazole or MDLA led to an increase in the uptake of Periodic Acid Schiff (PAS) stain in the glandular part of the gastric tissue, raised levels of prostaglandin E2 (PGE2) and superoxide dismutase (SOD), and a reduction in malondialdehyde (MDA) concentrations. These results suggested the gastroprotective action of Mn (II) complex.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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