Most studies have examined the association of ergonomic risk factors and musculoskeletal discomfort in developed countries. Meanwhile the data are still lacking in developing countries such as Malaysia. The aim of this study was to determine the relation between risk factors and musculoskeletal discomfort among manual material handling workers in Malaysian automotive industries. A total of211 manual material handling workers from automotive industries completed a set of questionnaire on the individual, physical and environmental factors and the prevalence of musculoskeletal discomfort. The Chi-Square test and logistics regression analysis were used to determine the relationship of the risk factors and musculoskeletal. The findings highlighted that job tenure was significantly correlated with musculoskeletal discomfort among the workers (OR=2.33-5.56). The most significant physical risk factor that was associated with musculoskeletal discomfort was bending the trunk forward slightly, hands above knee level, which was significantly related to lower back discomfort (OR=5.13, 95%CI=1.56-16.8), thigh discomfort (OR=5.1, 95%CI=1.01-25.53) and wrist discomfort (OR=3.65, 95%CI=1.06-12.53). Twisting of the trunk (over 45o) and bending sideways were significantly associated to lower back discomfort (OR=4.04, 95%CI=1.44-14.44), and thigh discomfort (OR=4.3, 95%CI=1.29-8.50). The findings also highlighted that environmental factors was associated with musculoskeletal discomfort (p < 0.05. Musculoskeletal discomfort can be reduced by lowering work-related risk factors among automotive manual material handling workers, particularly by focusing on significant factors, including job tenure, bending or twisting postures and environmental factors.