Methotrexate and beta-carotene loaded-lipid polymer hybrid nanoparticles: a preclinical study for breast cancer

Jain A 1 , Sharma G 1 , Kushwah V 2 , Garg NK 1 , Kesharwani P 3 , Ghoshal G 4 Show all authors , Singh B 4 , Shivhare US 4 , Jain S 2 , Katare OP 1

Affiliations 

  • 1 University Institute of Pharmaceutical Sciences, UGC-Centre of Advanced Studies, Panjab University, Chandigarh 160 014, India
  • 2 Department of Pharmaceutics, National Institute of Pharmaceutical Education & Research, SAS Nagar, Punjab 160 062, India
  • 3 Department of Pharmaceutical Technology, The International Medical University (IMU), Jalan Jalil Perkasa 19, Kuala Lumpur 57000, Malaysia
  • 4 Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh 160 014, India
Nanomedicine (Lond), 2017 Aug;12(15):1851-1872.
PMID: 28703643 DOI: 10.2217/nnm-2017-0011

Abstract

AIM: This work was intended to investigate the targeting potential of fructose-tethered lipid-polymeric hybrid nanoparticles (F-BC-MTX-LPHNPs) co-loaded with beta carotene (BC) and methotrexate (MTX) in breast cancer therapeutics and find out the possible protective role of BC on MTX-induced toxicity.

MATERIALS & METHODS: F-BC-MTX-LPHNPs were fabricated using self-assembled nano-precipitation technique. Fructose was conjugated on the surface of the particles. The in vitro cytotoxicity, sub-cellular localization and apoptotic activity of F-BC-MTX-LPHNPs were evaluated against MCF-7 breast cancer cells. The antitumor potential of F-BC-MTX-LPHNPs was further studied.

RESULTS & CONCLUSION: Outcomes suggested that F-BC-MTX-LPHNPs induced the highest apoptosis index (0.89) against MCF-7 cells. Following 30 days of treatment, the residual tumor progression was assessed to be approximately 32%, in animals treated with F-BC-MTX-LPHNPs. F-BC-MTX-LPHNPs are competent to selectively convey the chemotherapeutic agent to the breast cancers. Beta carotene ameliorated MTX-induced hepatic and renal toxicity.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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