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Establishment of in vitro and in vivo anti-colon cancer efficacy of essential oils containing oleo-gum resin extract of Mesua ferrea

Asif M 1 , Yehya AHS 2 , Dahham SS 3 , Mohamed SK 4 , Shafaei A 5 , Ezzat MO 6 Show all authors , Abdul Majid AS 7 , Oon CE 2 , Abdul Majid AMS 8

Affiliations 

  • 1 Faculty of Pharmaceutical Sciences, Government College University, 38000, Faisalabad, Pakistan; EMAN Testing and Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universti Sains Malaysia, Penang, 11800, Malaysia. Electronic address: Asif_pharmacist45@yahoo.com
  • 2 Institute for Research in Molecular Medicine (INFORMM), Universti Sains Malaysia, Penang, 11800, Malaysia
  • 3 Department of Science, Rustaq College of Education, Ministry of Higher Education, 329-Rustaq, Sultanate of Oman, Oman
  • 4 EMAN Testing and Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universti Sains Malaysia, Penang, 11800, Malaysia
  • 5 Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universti Sains Malaysia, Penang, 11800, Malaysia
  • 6 Department of Chemistry, College of Education for Women, University of Anbar, 31001, Ramadi, Anbar, Iraq
  • 7 Department of Pharmacology, School of Medical Sciences, Quest International University, Perak, Malaysia
  • 8 EMAN Testing and Research Laboratory, Department of Pharmacology, School of Pharmaceutical Sciences, Universti Sains Malaysia, Penang, 11800, Malaysia; ACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, Australian National University, Australia. Electronic address: Aminmalikshah@gmail.com
Biomed Pharmacother, 2019 Jan;109:1620-1629.
PMID: 30551416 DOI: 10.1016/j.biopha.2018.10.127

Abstract

Proven the great potential of essential oils as anticancer agents, the current study intended to explore molecular mechanisms responsible for in vitro and in vivo anti-colon cancer efficacy of essential oil containing oleo-gum resin extract (RH) of Mesua ferrea. MTT cell viability studies showed that RH had broad spectrum cytotoxic activities. However, it induced more profound growth inhibitory effects towards two human colon cancer cell lines i.e., HCT 116 and LIM1215 with an IC50 values of 17.38 ± 0.92 and 18.86 ± 0.80 μg/mL respectively. RH induced relatively less toxicity in normal human colon fibroblasts i.e., CCD-18co. Cell death studies conducted, revealed that RH induced characteristic morphological and biochemical changes in HCT 116. At protein level it down-regulated expression of multiple pro-survival proteins i.e., survivin, xIAP, HSP27, HSP60 and HSP70 and up-regulated expression of ROS, caspase-3/7 and TRAIL-R2 in HCT 116. Furthermore, significant reduction in invasion, migration and colony formation potential was observed in HCT 116 treated with RH. Chemical characterization by GC-MS and HPLC methods revealed isoledene and elemene as one the major compounds. RH showed potent antitumor activity in xenograft model. Overall, these findings suggest that RH holds a promise to be further studied for cheap anti-colon cancer naturaceutical development.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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