A potential marker in brucellosis, long non coding RNA IFNG-AS1

Gheitasi R 1 , Jourghasemi S 1 , Pakzad I 2 , Hosseinpour Sarmadi V 3 , Samieipour Y 4 , Sekawi Z 5 Show all authors , Azizi Jalilian F 6

Affiliations 

  • 1 Department of Immunology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  • 2 Department Microbiology, Faculty of Medicine and Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
  • 3 Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • 4 Institute of Virology, Technical University of Munich, Munich, Germany
  • 5 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia
  • 6 Department of Medical Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. azizifarid@gmail.com
Mol Biol Rep, 2019 Dec;46(6):6495-6500.
PMID: 31595441 DOI: 10.1007/s11033-019-05095-w

Abstract

Brucellosis is the most common bacterial zoonotic infection. This pathogen may survive and sustain in host. The aim of this study is to define relationship between long noncoding (lnc) RNA-IFNG-AS1 and interferon gamma (IFN-γ) in different groups of patients with brucellosis compared to control group. In this study, associations of lncRNA IFNG-AS1 expression with secretion of IFN-γ level in Sixty patients with brucellosis, which were divided into 3 groups (acute, chronic and relapse groups), as a case group were compared with 20 subjects with negative serological tests and brucellosis clinical manifestation as a control group. In this regard, RNA were extracted from isolated peripheral blood mononuclear cells (PBMCs). LncRNA IFNG-AS1, T-box transcription factor (T-bet) and IFN-γ expressions were detected using quantitative polymerase chain reaction (qPCR). Serum level IFN-γ was assessed using enzyme linked immunosorbent assay (ELISA). The results showed that expression level of LncRNA IFNG-AS1, T-bet and IFN-γ increased significantly in all patient groups in compared to healthy subjects (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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