Affiliations 

  • 1 Department of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway, Malaysia
  • 2 Department of Clinical Sciences, College of Medicine, University of Sharjah, University City, Sharjah, United Arab Emirates. naveed5438@gmail.com
  • 3 Monash University Malaysia Genomics Facility, School of Science, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia
  • 4 Department of Biological Sciences, School of Science and Technology, Sunway University, Bandar Sunway, Malaysia. ayazanwarkk@yahoo.com
  • 5 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan
  • 6 College of Arts and Sciences, American University of Sharjah, University City, Sharjah, United Arab Emirates
BMC Microbiol, 2021 Feb 17;21(1):51.
PMID: 33596837 DOI: 10.1186/s12866-021-02097-2

Abstract

BACKGROUNDS: Escherichia coli K1 causes neonatal meningitis. Transcriptome studies are indispensable to comprehend the pathology and biology of these bacteria. Recently, we showed that nanoparticles loaded with Hesperidin are potential novel antibacterial agents against E. coli K1. Here, bacteria were treated with and without Hesperidin conjugated with silver nanoparticles, and silver alone, and 50% minimum inhibitory concentration was determined. Differential gene expression analysis using RNA-seq, was performed using Degust software and a set of genes involved in cell stress response and metabolism were selected for the study.

RESULTS: 50% minimum inhibitory concentration with silver-conjugated Hesperidin was achieved with 0.5 μg/ml of Hesperidin conjugated with silver nanoparticles at 1 h. Differential genetic analysis revealed the expression of 122 genes (≥ 2-log FC, P

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.