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Clinical and Radiological Evaluation of Sinus Membrane Osteogenicity Subsequent to Internal Sinus Lifting and Implant Placement

Qabbani AA, Bayatti SWA, Hasan H, Samsudin AB, Kawas SA

J Craniofac Surg, 2020 1 3;31(3):e233-e236.
PMID: 31895847 DOI: 10.1097/SCS.0000000000006106

To evaluate the ability of the maxillary sinus membrane to produce bone after internal sinus lifting and implant placement without adding exogenous bone graft, and to assess the quality of bone that has been produced 6 months postoperatively.In this retrospective study, 10 subjects who underwent maxillary sinus floor lifting and met the inclusion criteria were selected and then subdivided into: Group-A underwent internal sinus elevation and placement of implants without the use of bone graft and Group-B underwent classical internal sinus lifting and placement of bovine bone particles and then placement of the implant as a control group. Radiofrequency analysis (RFA) values for measuring the Implant Stability Quotient (ISQ) of all implants were measured by Osstell device. CBCT was performed involving linear measurements of the site of sinus lifting for both groups.High RFA values demonstrating excellent biomechanical stability were observed in Group-A compared to Group-B at 6 months postoperatively. Group-A showed a median of ISQ value;78 (8), 77(12), 79(3.5) and 77(4.50). Group-A was significantly higher in ISQ values than Group-B, which showed median and interquartile range (IQR) of ISQ value of [51(12.50), 54(14.50), 55(9), and 55(7.50)]. However, the amount of bone available in group B was significantly higher than group A; [3.5 mm (0.75) and 3.8 mm (0.69)].Internal sinus lifting without bone graft has the ability of bone formation by osteogenic potential of the sinus membrane. Nevertheless, the high quality of bone being produced is of high importance for the success of an implant without the need for an exogenous bone graft. The newly formed bone was significantly of a better quality in Group-A. Thus, it is recommended to perform internal sinus lifting, without adding bone graft material and allow the osteogenic potential of the maxillary sinus membrane to produce its own osteogenic cells.

Matched MeSH terms: Cattle
Fulltext Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis

Moura de Aguiar D, Pessoa Araújo Junior J, Nakazato L, Bard E, Aguilar-Bultet L, Vorimore F, et al.

Microorganisms, 2019 Nov 05;7(11).
PMID: 31694172 DOI: 10.3390/microorganisms7110528

The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichiaminasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the 'clustering-based subsystems' category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.

Matched MeSH terms: Cattle
Fulltext Evaluation of Adsorptive Characteristics of Cow Dung and Rice Husk Ash for Removal of Aqueous Glyphosate and Aminomethylphoshonic Acid

Garba J, Samsuri WA, Othman R, Hamdani MSA

Sci Rep, 2019 11 27;9(1):17689.
PMID: 31776374 DOI: 10.1038/s41598-019-54079-0

Glyphosate (GLY) is a major herbicide used throughout the world, and its continuous application has become an environmental issue. Adsorption is an important mechanism for removing organic contaminant in water. The present study characterized cow dung (CD) and rice husk ash (RHA), and determined the adsorption-desorption of GLY and its metabolite, aminomethylphoshonic acid (AMPA), on to them. The results revealed that both CD and RHA were alkaline and had no or low content of arsenic, cadmium, chromium and lead. The CD had lower surface area (13.104 mg2g-1) than RHA (21.500 m2g-1). The CD contained amines, phenol, ethers and carboxylic functional groups, while in addition to carboxylic and ether, RHA contains siloxane. Both CD and RHA had high affinities for GLY and AMPA. The Freundlich sorption coefficient (Kf) on AMPA were 2.915 and 2.660 for CD and RHA, respectively, while the values on GLY were 1.168 and 1.166 (mg g-1) for CD and RHA, respectively. Desorption of GLY only occurred at lower concentrations, while no desorption of AMPA was recorded, indicating their strong adsorption on CD and RHA. Considering their availabilities and affordable prices, both CD and RHA can be recommended as economical adsorbent for the removal of GLY and AMPA.

Matched MeSH terms: Cattle
Fulltext A Comparative Study of Biogas Production from Cattle Slaughterhouse Wastewater Using Conventional and Modified Upflow Anaerobic Sludge Blanket (UASB) Reactors

Musa MA, Idrus S, Harun MR, Tuan Mohd Marzuki TF, Abdul Wahab AM

Int J Environ Res Public Health, 2019 12 31;17(1).
PMID: 31906118 DOI: 10.3390/ijerph17010283

Cattle slaughterhouses generate wastewater that is rich in organic contaminant and nutrients, which is considered as high strength wastewater with a high potential for energy recovery. Work was undertaken to evaluate the efficiency of the 12 L laboratory scale conventional and a modified upflow anaerobic sludge blanket (UASB) reactors (conventional, R1 and modified, R2), for treatment of cattle slaughterhouse wastewater (CSWW) under mesophilic condition (35 ± 1 °C). Both reactors were acclimated with synthetic wastewater for 30 days, then continuous study with real CSWW proceeds. The reactors were subjected to the same loading condition of OLR, starting from 1.75, 3, 5 10, 14, and 16 g L-1d-1, corresponding to 3.5, 6, 10, 20, 28, and 32 g COD/L at constant hydraulic retention time (HRT) of 24 h. The performance of the R1 reactor drastically dropped at OLR 10 g L-1d-1, and this significantly affected the subsequent stages. The steady-state performance of the R2 reactor under the same loading condition as the R1 reactor revealed a high COD removal efficiency of 94% and biogas and methane productions were 27 L/d and 89%. The SMP was 0.21 LCH4/gCOD added, whereas the NH3-N alkalinity ratio stood at 651 mg/L and 0.2. SEM showed that the R2 reactor was dominated by Methanosarcina bacterial species, while the R1 reactor revealed a disturb sludge with insufficient microbial biomass.

Matched MeSH terms: Cattle
Hydroxyproline determination for initial detection of halal-critical food ingredients (gelatin and collagen)

Yuswan MH, A Jalil NH, Mohamad H, Keso S, Mohamad NA, Tengku Md Yusoff TS, et al.

Food Chem, 2021 Feb 01;337:127762.
PMID: 32777563 DOI: 10.1016/j.foodchem.2020.127762

Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs.

Matched MeSH terms: Cattle
Fulltext Claw Trimming as a Lameness Management Practice and the Association with Welfare and Production in Dairy Cows

Sadiq MB, Ramanoon SZ, Mansor R, Syed-Hussain SS, Shaik Mossadeq WM

Animals (Basel), 2020 Aug 27;10(9).
PMID: 32867064 DOI: 10.3390/ani10091515

Lameness resulting from claw lesions remains a pressing welfare issue in dairy cows. Claw trimming (CT) is a common practice for prevention and management of clinically lame cows. This review summarizes the results of studies that have investigated various claw trimming (CT) methods, their application in lameness management, and associations with the welfare and production of dairy cows. The papers included in this review fulfilled the following inclusion criteria: published in peer review journal or book chapter within the last 20 years (1999-2019), written in English, and focused on the application of CT for lameness management and the association with either welfare or production variables. Databases used included Google scholar, Web of Science and PubMed. A total of 748 records were assessed and 61 papers were eligible for inclusion and the main objectives and results were used to categorize the results under six topics: CT techniques, association between CT and claw overgrowth/specific claw lesions, timing and frequency of CT, association between CT and behavioral variables, association between CT and physiological parameters, and association between CT and production. The literature findings showed the existence of various CT methods with the common types including the Dutch Five-step, White Line, White Line Atlas, and Kansas techniques. There is data paucity on the efficacy of these techniques in lameness management; however, the slight procedural difference yields varying sole thicknesses and presentations which may influence their prophylactic use. Results regarding the impact of CT on welfare and production were discussed in relation to potential short and long-term benefits. Depending on the lesion type and severity level, CT may induce immediate painful sensation, stress, changes in lying down activities and reduction in milk yield, but the positive impacts were more evident at later stages of lactation following improvement in locomotion score. The majority of the reviewed studies were lacking a detailed description of CT techniques and claw health of the studied animals; thus, reducing the strength of demonstrating CT-related benefits. However, electronic recording of claw health data during every CT visit provides the basis for monitoring hoof health and could assist in curtailing some of these challenges. To elucidate CT-related benefits, certain areas requiring further research were highlighted such as ascertaining the appropriate timing for preventive CT and identifying cows that will benefit more from such intervention during lactation.

Matched MeSH terms: Cattle
Fulltext The Inhibitory Effects of Heterotrigona Itama Honey Marinades on the Formation of Carcinogenic Heterocyclic Amines in Grilled Beef Satay

Shamsudin S, Selamat J, Sanny M, Jambari NN, Sukor R, Praveena SM, et al.

Molecules, 2020 Aug 26;25(17).
PMID: 32858787 DOI: 10.3390/molecules25173874

Heterocyclic amines (HCAs) are carcinogenic food toxicants formed in cooked meats, which may increase the risk of cancer development in humans. Therefore, in this study, the effect of stingless bee honey from different botanical origins on the formation of HCAs in grilled beef satay was investigated. HCAs concentration in grilled beef satay was determined by using high performance liquid chromatography (HPLC). In total, six of the most toxigenic HCAs representing aminoimidazo-azaarenes (AIAs) (MeIQx, 4,8-DiMeIQx, and PhIP) and amino carbolines (norharman, harman, and AαC) groups were identified in all the beef samples investigated. A significant reduction in HCAs was observed in grilled beef marinated in honey as compared to beef samples marinated in table sugar (control), in which the reduction of 95.14%, 88.45%, 85.65%, and 57.22% was observed in gelam, starfruit, acacia, and Apis honey marinades, respectively. According to the partial least squares regression (PLS) model, the inhibition of HCAs in grilled beef was shown to be significantly correlated to the antioxidant activity (IC50) of the honey samples. Therefore, the results of this study revealed that the addition of stingless bee honey could play an important role in reducing HCAs in grilled beef.

Matched MeSH terms: Cattle
Fulltext Current and future trends in socio-economic, demographic and governance factors affecting global primate conservation

Estrada A, Garber PA, Chaudhary A

PeerJ, 2020;8:e9816.
PMID: 32884865 DOI: 10.7717/peerj.9816

Currently, ~65% of extant primate species (ca 512 species) distributed in 91 countries in the Neotropics, mainland Africa, Madagascar, South Asia and Southeast Asia are threatened with extinction and 75% have declining populations as a result of deforestation and habitat loss resulting from increasing global market demands, and land conversion for industrial agriculture, cattle production and natural resource extraction. Other pressures that negatively impact primates are unsustainable bushmeat hunting, the illegal trade of primates as pets and as body parts, expanding road networks in previously isolated areas, zoonotic disease transmission and climate change. Here we examine current and future trends in several socio-economic factors directly or indirectly affecting primates to further our understanding of the interdependent relationship between human well-being, sustainable development, and primate population persistence. We found that between 2001 and 2018 ca 191 Mha of tropical forest (30% canopy cover) were lost as a result of human activities in the five primate range regions. Forty-six percent of this loss was in the Neotropics (Mexico, Central and South America), 30% in Southeast Asia, 21% in mainland Africa, 2% in Madagascar and 1% in South Asia. Countries with the greatest losses (ca 57% of total tree cover loss) were Brazil, Indonesia, DRC, China, and Malaysia. Together these countries harbor almost 50% of all extant primate species. In 2018, the world human population was estimated at ca 8bn people, ca 60% of which were found in primate range countries. Projections to 2050 and to 2100 indicate continued rapid growth of the human populations in these five primate range regions, with Africa surpassing all the other regions and totaling ca 4bn people by the year 2100. Socioeconomic indicators show that, compared to developed nations, most primate range countries are characterized by high levels of poverty and income inequality, low human development, low food security, high levels of corruption and weak governance. Models of Shared Socioeconomic Pathway scenarios (SSPs) projected to 2050 and 2100 showed that whereas practices of increasing inequality (SSP4) or unconstrained growth in economic output and energy use (SSP5) are projected to have dire consequences for human well-being and primate survivorship, practices of sustainability-focused growth and equality (SSP1) are expected to have a positive effect on maintaining biodiversity, protecting environments, and improving the human condition. These results stress that improving the well-being, health, and security of the current and future human populations in primate range countries are of paramount importance if we are to move forward with effective policies to protect the world's primate species and promote biodiversity conservation.

Matched MeSH terms: Cattle
Fulltext Are bovine and porcine adenoviruses distinctive from other adenovirus strains?

Florence C. H. Lee, Lu Ping Tan

Malaysian Journal of Medicine and Health Sciences, 2019;15(106):86-86.
MyJurnal

Introduction: Adenoviruses are important in public health. They are used as indicators for microbial pollution source tracking (MST), and as vaccine vectors. Knowledge of host specificity and pre-existing immunity are critical for the two applications. Bovine and porcine adenoviruses are expected to be widespread in the environment through agriculture. Here we examined if bovine and porcine adenovirus strains are truly distinctive from other adenovirus strains. Methods: 46 adenovirus strains with complete genome were downloaded from the RefSeq database of Gen-Bank. 450 bp of nucleotides each at positions 1- 450, 18001-18450, and 27001 – 27450 of the respective strains were subjected to the Disparity Index Test using Mega 7 software. Analysis focused on four adenovirus strains: Bo-vine B, Bovine D, Porcine C and Porcine 3. The total number of strains that were significantly different (P

Matched MeSH terms: Cattle
Fulltext Effects of Different Types of Plant-Based Milk and Animal-Based Milk Delivery Vehicle on Growth of Lactobacillus Plantarum

Nurhazirah Salehen, Nurzafirah Mazlan, Oon, Chuah Li, Siti Marwanis Anua,, Young, Thung Tze

Journal of Management & Science, 2018;16(2):67-73.
MyJurnal

Probiotics has been discovered long time ago for its beneficial effect on health when consumed especially to the people who had allergy and gastrointestinal disease. This preliminary study was conducted to find out which vehicles can allow better growth of probiotic. Lactobacillus plantarum was used in this study as choice of probiotic to be cultured in the four types of milk. The pH value was recorded for determination of growths and metabolic activity of the probiotic. Results showed that L. plantarum in soy milk can grow and had a better metabolic activity in the cultured soy milk with pH 3.46 compared to others. The highest growth was recorded at optical density of 1.137 in soy milk at 560nm. This result showed that L. plantarum in soy milk multiply rapidly compared to other milks. As conclusion, soy milk has better development of probiotic as delivery vehicles compare to cow milk, goat milk and coconut milk.

Matched MeSH terms: Cattle
A novel wound healing accelerator: Effect of vitreous gel of cow eyeball on a chronic wound model

Makhmudi A, Wirohadidjojo YW, Gahara E, Noor HZ, Sunardi M, Mahmudah NA, et al.

Med J Malaysia, 2020 11;75(6):698-704.
PMID: 33219180

INTRODUCTION: Several studies have reported the disturbance in the process of wound healing after administration of mitomycin-C, which inhibits granulation tissue formation and collagen synthesis, resulting in chronic wounds. The vitreous gel of cow eyeballs contains a high level of hyaluronic acid, which has a role in inflammation, granulation, re-epithelialization, and remodelling. This study aims to understand the effect of 1% povidone iodine and vitreous gel of cow eyeballs on wound healing after administration of mitomycin-C.
METHODS: This was an in vivo study with quasi-experimental methods on 32 Wistar mice. Full-thickness wounds were made and then treated with mitomicyn-C. The mice were divided into 4 groups: a control group with NaCl 0.9% vitreous gel of cow eyeball (VGCE), 1% povidone-iodine, and a combination of VGCE and 1% povidone-iodine groups. Macroscopic and microscopic observations of the process of wound healing were performed on days 3, 7, and 14.
RESULTS: Vitreous gel administration produced significant wound healing rates within the first three days, and histological analysis revealed an increased number of fibroblasts and polymorphonuclear cells. However, the povidone iodine group and the combination group with vitreous gel did not produce significant results.
CONCLUSION: The single administration of VGCE can accelerate the wound healing process, increase the number of fibroblasts, and reduce inflammation in a chronic wound model.

Matched MeSH terms: Cattle
Detection of Escherichia coli O157:H7 in the beef marketed in Malaysia

Radu S, Abdul Mutalib S, Rusul G, Ahmad Z, Morigaki T, Asai N, et al.

Appl Environ Microbiol, 1998 Mar;64(3):1153-6.
PMID: 9501454

Twelve strains of Escherichia coli O157:H7 were isolated from 9 of 25 beef samples purchased from retail stores in Malaysia. These strains produced Shiga toxin 2 with or without Shiga toxin 1 and had the eae gene and a 60-MDa plasmid. The antibiograms and the profiles of the arbitrarily primed PCR of the strains were diverse, suggesting that the strains may have originated from diverse sources.

Matched MeSH terms: Cattle
Antifouling polyethersulfone hemodialysis membranes incorporated with poly (citric acid) polymerized multi-walled carbon nanotubes

Abidin MNZ, Goh PS, Ismail AF, Othman MHD, Hasbullah H, Said N, et al.

Mater Sci Eng C Mater Biol Appl, 2016 Nov 01;68:540-550.
PMID: 27524052 DOI: 10.1016/j.msec.2016.06.039

Poly (citric acid)-grafted-MWCNT (PCA-g-MWCNT) was incorporated as nanofiller in polyethersulfone (PES) to produce hemodialysis mixed matrix membrane (MMM). Citric acid monohydrate was polymerized onto the surface of MWCNTs by polycondensation. Neat PES membrane and PES/MWCNTs MMMs were fabricated by dry-wet spinning technique. The membranes were characterized in terms of morphology, pure water flux (PWF) and bovine serum albumin (BSA) protein rejection. The grafting yield of PCA onto MWCNTs was calculated as 149.2%. The decrease of contact angle from 77.56° to 56.06° for PES/PCA-g-MWCNTs membrane indicated the increase in surface hydrophilicity, which rendered positive impacts on the PWF and BSA rejection of the membrane. The PWF increased from 15.8Lm(-2)h(-1) to 95.36Lm(-2)h(-1) upon the incorporation of PCA-g-MWCNTs due to the attachment of abundant hydrophilic groups that present on the MWCNTs, which have improved the affinity of membrane towards the water molecules. For protein rejection, the PES/PCA-g-MWCNTs MMM rejected 95.2% of BSA whereas neat PES membrane demonstrated protein rejection of 90.2%. Compared to commercial PES hemodialysis membrane, the PES/PCA-g-MWCNTs MMMs showed less flux decline behavior and better PWF recovery ratio, suggesting that the membrane antifouling performance was improved. The incorporation of PCA-g-MWCNTs enhanced the separation features and antifouling capabilities of the PES membrane for hemodialysis application.

Matched MeSH terms: Cattle
Isotopic ratio analysis of cattle tail hair: A potential tool in building the database for cow milk geographical traceability

Behkami S, Zain SM, Gholami M, Bakirdere S

Food Chem, 2017 Feb 15;217:438-444.
PMID: 27664656 DOI: 10.1016/j.foodchem.2016.08.130

The potential for the isotopic ratio analysis of cattle tail hair in determining the geographical origin of raw cow milk in Peninsular Malaysia had been investigated in this research using exploratory visualization. A significant positive correlation (p<0.0001) (n=54) was noticed between δ(13)C and δ(15)N in milk with that of hair which indicated that these matrices could be used in tracing the geographical origin of animal produce and tissues, and there is a possibility that hair could be used as a substitute in building the database for the geographical traceability of milk. It was also observed that both hair and milk isotopic ratio correlations exhibited separation between the northern and southern regions. The accuracy of using isotopic ratio in determining geographical discrimination had been clearly demonstrated when several commercial milk samples from the same regions under the study were correctly assigned to the appropriate geographical clusters.

Matched MeSH terms: Cattle
Fulltext Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk

Lye, Y.L., Afsah-Hejri, L., Chang, W.S., Loo, Y.Y., Puspanadan, S., Kuan, C.H., et al.

International Food Research Journal, 2013;20(2):1001-1005.
MyJurnal

E. coli O157:H7 is associated with life threatening diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Raw milk is considered a high risk food as it is highly nutritious and serves as an ideal medium for bacterial growth. The aim of this study was to investigate the prevalence of E. coli O157:H7 in raw cow, goat and buffalo milk samples. MPN-PCR method targeting the major virulence rfbE gene and fliCH7gene of E. coli O157:H7 was used. Total of 177 raw milk samples were collected from local dairy farms in the state of Selangor, Malaysia. The highest prevalence of E. coli O157:H7 was found in raw cow milk (18.75%). E. coli O157:H7 was detected in 7.32% and 3.57% of raw goat and buffalo milk, respectively. The estimated quantity of E. coli O157:H7 in raw cow, goat and buffalo milk ranged from

Matched MeSH terms: Cattle
Fulltext Effects of different drying methods on the rheological, functional and structural properties of chicken skin gelatin compared to bovine gelatin

Rasli, H.I., Sarbon, N.M.

International Food Research Journal, 2015;22(2):584-592.
MyJurnal

This study investigates effects from different drying methods (vacuum oven dried vs. freeze dried) on the rheological, functional and structural properties of chicken skin gelatin compared to bovine gelatin. Vacuum oven dried chicken skin samples showed a higher gelatin yield (12.86%) than freeze-dried samples (9.25%). The latter showed a higher melting temperature (32.64oC) and superior foaming capacity (176%) as well as foaming stability (166.67%). Vacuum oven dried samples demonstrated greater fat binding capacity (5.5 ml/g) and emulsion stability (55.79%). There were no significant differences (p >0.05) in emulsion and water holding capacity for three gelatins. Bovine gelatin did hold the lowest of all functional properties studied. A Fourier Transform Infrared (FTIR) spectrum analysis of chicken skin gelatin under both drying methods presented structures similar to those of bovine gelatin. Collectively, this findings indicated no significant differences (p >0.05) in rheological, functional and structural properties for chicken skin gelatins prepared by either drying method. Hence, to save costs and maintain gelatin quality, vacuum oven drying offers potential as an alternative means of production.

Matched MeSH terms: Cattle
Fulltext Physicochemical and structural properties of Asian Swamp Eel (Monopterus albus) skin gelatin as compared to bovine gelatin

Rosli, N., Sarbon, N.M.

International Food Research Journal, 2015;22(2):699-706.
MyJurnal

The aims of this study are to report on the extraction and characterization of Asian swamp eel (Monopterus albus) skin gelatin. The characterization conducted were includes chemical composition, pH, gel strength, viscosity, thermal property, color and structure determination of extracted eel skin gelatin. Eel skin contains 70.28% moisture, 11.07% protein, 4.21% fat, and 5.01% ash. The chemical composition of eel skin gelatin (yield of 12.75%) was 18.8% moisture, 67.64% protein, 0.34% fat and 1.08% ash, with a pH of 4.62 and gel strength of 215.96 g (± 9.62 g). Although viscosity (2.8 cPa/min) profile of eel skin gelatin showed lower than that of bovine gelatin, the higher melting temperature (35 °C) of eel skin gelatin indicating its higher stability than bovine gelatin with FTIR spectrum similar to that of typical bovine gelatin. Eel skin gelatin has a 71.4 (± 1.14), a +3.2 (± 0.29), and a +7.52 (± 0.29) for L*, a* and b* value respectively, indicate a darker and less yellow colour. These findings show promising potential for the application of eel skin gelatin as an alternative to commercial gelatin.

Matched MeSH terms: Cattle
Fulltext Extraction and characterization of pepsin-solubilized collagen from the body wall of crown-of-thorns Starfish (Acanthaster planci)

Tan, C. C., Karim, A. A., Latiff, A. A., Gan, C. Y., Ghazali, F. C.

International Food Research Journal, 2013;20(6):3013-3020.
MyJurnal

Pepsin-solubilized collagen (PSC) was extracted from the body wall of crown-of-thorns starfish (COTS) (Acanthaster planci) using pepsin digestion in 0.5 M acetic acid. The electrophoretic pattern of PSC showed that it consisted of two α chains (α1 and α2 chains). In addition, the peptide mapping showed that there were some differences in peptide patterns among PSC, calf skin collagen and salmon skin collagen. This suggested that the primary structure of the PSC was different from calf skin collagen and salmon skin collagen. Furthermore, Fourier transform infrared spectroscopy (FTIR) investigation showed the existence of triple helical structure in PSC, suggesting that the triple helical structure was well preserved during the extraction of collagen from COTS. The denaturation temperature of PSC was 33.0°C, which was comparable with mammalian collagen. In addition, the amino acid composition analysis showed that the imino acid content of PSC was similar to mammalian collagen but it was higher than other marine collagen. This suggested that the imino acid content might contributed the denaturation temperature of collagen. The results in this study suggest that PSC from the underutilized COTS has potential to be exploited in various commercial applications.

Matched MeSH terms: Cattle
Ganglioside Composition in Beef, Chicken, Pork, and Fish Determined Using Liquid Chromatography-High-Resolution Mass Spectrometry

Fong BY, Ma L, Khor GL, van der Does Y, Rowan A, McJarrow P, et al.

J Agric Food Chem, 2016 Aug 17;64(32):6295-305.
PMID: 27436425 DOI: 10.1021/acs.jafc.6b02200

Gangliosides (GA) are found in animal tissues and fluids, such as blood and milk. These sialo-glycosphingolipids have bioactivities in neural development, the gastrointestinal tract, and the immune system. In this study, a high-performance liquid chromatography-mass spectrometry (HPLC-MS) method was validated to characterize and quantitate the GA in beef, chicken, pork, and fish species (turbot, snapper, king salmon, and island mackerel). For the first time, we report the concentration of GM3, the dominant GA in these foods, as ranging from 0.35 to 1.1 mg/100 g and 0.70 to 5.86 mg/100 g of meat and fish, respectively. The minor GAs measured were GD3, GD1a, GD1b, and GT1b. Molecular species distribution revealed that the GA contained long- to very-long-chain acyl fatty acids attached to the ceramide moiety. Fish GA contained only N-acetylneuraminic acid (NeuAc) sialic acid, while beef, chicken, and pork contained GD1a/b species that incorporated both NeuAc and N-glycolylneuraminic acid (NeuGc) and hydroxylated fatty acids.

Matched MeSH terms: Cattle
Fulltext Milk derived bioactive peptides and their impact on human health - A review

Mohanty DP, Mohapatra S, Misra S, Sahu PS

Saudi J Biol Sci, 2016 Sep;23(5):577-83.
PMID: 27579006 DOI: 10.1016/j.sjbs.2015.06.005

Milk-derived bioactive peptides have been identified as potential ingredients of health-promoting functional foods. These bioactive peptides are targeted at diet-related chronic diseases especially the non-communicable diseases viz., obesity, cardiovascular diseases and diabetes. Peptides derived from the milk of cow, goat, sheep, buffalo and camel exert multifunctional properties, including anti-microbial, immune modulatory, anti-oxidant, inhibitory effect on enzymes, anti-thrombotic, and antagonistic activities against various toxic agents. Majority of those regulate immunological, gastrointestinal, hormonal and neurological responses, thereby playing a vital role in the prevention of cancer, osteoporosis, hypertension and other disorders as discussed in this review. For the commercial production of such novel bioactive peptides large scale technologies based on membrane separation and ion exchange chromatography methods have been developed. Separation and identification of those peptides and their pharmacodynamic parameters are necessary to transfer their potent functional properties into food applications. The present review summarizes the preliminary classes of bioactive milk-derived peptides along with their physiological functions, general characteristics and potential applications in health-care.

Matched MeSH terms: Cattle

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