Displaying publications 2881 - 2900 of 9872 in total

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  1. Nah SA, Tan HL, Tamba RP, Aziz DA, Azzam N
    J Pediatr Surg, 2011 Feb;46(2):424-7.
    PMID: 21292104 DOI: 10.1016/j.jpedsurg.2010.11.045
    Necrotizing enterocolitis has a wide clinical spectrum of manifestation. We report a novel method of managing focal isolated perforation in necrotizing enterocolitis by using diagnostic laparoscopy to localize the site of perforation and by making a microincision over the perforation to perform exteriorization or limited resection and primary anastomosis.
    Matched MeSH terms: Laparotomy/methods*; Microsurgery/methods; Laparoscopy/methods*
  2. Beng TS, Chin LE
    J Palliat Med, 2010 Aug;13(8):1037-8.
    PMID: 20712471 DOI: 10.1089/jpm.2009.0314
    We present a case of gross lower limb edema in a 21-year-old man with an intra-abdominal malignant fibrous histiocytoma. He had a 1-month history of lower limb edema secondary to inferior vena caval obstruction. His edema failed to respond to a combination of diuretics, oral frusemide 40 mg daily and oral spironolactone 100 mg daily. Subcutaneous drainage of both lower limbs with butterfly needles was performed with minimal improvement. However, he gained significant symptomatic relief with multiple subcutaneous punctures and stoma bag drainage. A total of 12.8 L of serous fluid was drained with this simple and effective method. This is the first report of the use of this method.
    Matched MeSH terms: Drainage/methods*; Palliative Care/methods*; Punctures/methods*
  3. Hwang WH, Shen TJ
    Biometrics, 2010 Dec;66(4):1052-60.
    PMID: 20002401 DOI: 10.1111/j.1541-0420.2009.01371.x
    Many well-known methods are available for estimating the number of species in a forest community. However, most existing methods result in considerable negative bias in applications, where field surveys typically represent only a small fraction of sampled communities. This article develops a new method based on sampling with replacement to estimate species richness via the generalized jackknife procedure. The proposed estimator yields small bias and reasonably accurate interval estimation even with small samples. The performance of the proposed estimator is compared with several typical estimators via simulation study using two complete census datasets from Panama and Malaysia.
    Matched MeSH terms: Methods
  4. Yusof NM, Oh CW, Oh JK, Kim JW, Min WK, Park IH, et al.
    Injury, 2009 Dec;40(12):1286-91.
    PMID: 19539282 DOI: 10.1016/j.injury.2009.02.020
    Although non-operative treatment is a mainstay of tibial fracture management in children, certain fractures require a surgical approach. However, choices concerning optimal methods and implants are difficult. The purpose of this study was to determine the effectiveness of percutaneous plating of tibial fractures in children.
    Matched MeSH terms: Fracture Fixation, Internal/methods*; Minimally Invasive Surgical Procedures/methods; Prosthesis Implantation/methods
  5. Halim AA, Aziz HA, Johari MA, Ariffin KS, Adlan MN
    J Hazard Mater, 2010 Mar 15;175(1-3):960-4.
    PMID: 19945216 DOI: 10.1016/j.jhazmat.2009.10.103
    The performance of a carbon-mineral composite adsorbent used in a fixed bed column for the removal of ammoniacal nitrogen and aggregate organic pollutant (COD), which are commonly found in landfill leachate, was evaluated. The breakthrough capacities for ammoniacal nitrogen and COD adsorption were 4.46 and 3.23 mg/g, respectively. Additionally, the optimum empty bed contact time (EBCT) was 75 min. The column efficiency for ammoniacal nitrogen and COD adsorption using fresh adsorbent was 86.4% and 92.6%, respectively, and these values increased to 90.0% and 93.7%, respectively, after the regeneration process.
    Matched MeSH terms: Refuse Disposal/methods*; Waste Disposal, Fluid/methods; Water Purification/methods
  6. Koay AC, Yew YH, Ngo CT, Loo VP, Intan G, Chua CN
    Med J Malaysia, 2008 Dec;63(5):421-2.
    PMID: 19803308 MyJurnal
    Corneal perforation is an ocular emergency that requires early intervention to avoid permanent visual loss from endophthalmitis or irreversible structural changes. Although tectonic keratoplasty is the best choice for patching a large perforation, a donor cornea is not always immediately available. Consequently, an alternative material is required while awaiting a donor cornea. We report the use of preserved bovine pericardium as a temporizing graft to maintain the ocular integrity in two patients with corneal perforation.
    Matched MeSH terms: Emergency Treatment/methods; Transplantation, Heterologous/methods*; Corneal Transplantation/methods*
  7. Yong VC, Ong KW, Sidik SM, Rosli R, Chong PP
    J Microbiol Methods, 2009 Nov;79(2):242-5.
    PMID: 19737582 DOI: 10.1016/j.mimet.2009.08.019
    In situ Reverse Transcriptase PCR (in situ RT-PCR) can amplify mRNA and localize gene expression in cells. However, this method is not feasible in fungi as the thick fungal cell wall constitutes a barrier to this procedure. We developed a two step in situ RT-PCR procedure which enabled the detection and localization of Candida tropicalis mRNA expression in formalin-fixed, paraffin-embedded (FFPE) mouse kidney sections. This in situ hybridization study revealed the first direct evidence for deposition of Candida tropicalis secreted aspartic proteinase 2 (CtSAP2) in the tip of pseudohyphae and its involvement in acute systemic candidiasis. We conclude that in situ RT-PCR can be successfully applied to FFPE tissues and will offer new perspectives in studying gene expression in Candida species.
    Matched MeSH terms: Pathology/methods*; In Situ Hybridization/methods*; Reverse Transcriptase Polymerase Chain Reaction/methods*
  8. See HH, Marsin Sanagi M, Ibrahim WA, Naim AA
    J Chromatogr A, 2010 Mar 12;1217(11):1767-72.
    PMID: 20138287 DOI: 10.1016/j.chroma.2010.01.053
    A novel microextraction technique termed solid phase membrane tip extraction (SPMTE) was developed. Selected triazine herbicides were employed as model compounds to evaluate the extraction performance and multiwall carbon nanotubes (MWCNTs) were used as the adsorbent enclosed in SPMTE device. The SPMTE procedure was performed in semi-automated dynamic mode and several important extraction parameters were comprehensively optimized. Under the optimum extraction conditions, the method showed good linearity in the range of 1-100 microg/L, acceptable reproducibility (RSD 6-8%, n=5), low limits of detection (0.2-0.5 microg/L), and satisfactory relative recoveries (95-101%). The SPMTE device could be regenerated and reused up to 15 analyses with no analyte carry-over effects observed. Comparison was made with commercially available solid phase extraction-molecular imprinted polymer cartridge (SPE-MIP) for triazine herbicides as the reference method. The new developed method showed comparable or even better results against reference method and is a simple, feasible, and cost effective microextraction technique.
    Matched MeSH terms: Chromatography, Liquid/methods*; Solid Phase Extraction/methods*; Microtechnology/methods
  9. Ibrahim Z, Amin MF, Yahya A, Aris A, Muda K
    Water Sci Technol, 2010;61(5):1279-88.
    PMID: 20220250 DOI: 10.2166/wst.2010.021
    Textile wastewater, one of the most polluted industrial effluents, generally contains substantial amount of dyes and chemicals that will cause increase in the COD, colour and toxicity of receiving water bodies if not properly treated. Current treatment methods include chemical and biological processes; the efficiency of the biological treatment method however, remains uncertain since the discharged effluent is still highly coloured. In this study, granules consisting mixed culture of decolourising bacteria were developed and the physical and morphological characteristics were determined. After the sixth week of development, the granules were 3-10 mm in diameter, having good settling property with settling velocity of 70 m/h, sludge volume index (SVI) of 90 to 130 mL/g, integrity coefficient of 3.7, and density of 66 g/l. Their abilities to treat sterilised raw textile wastewater were evaluated based on the removal efficiencies of COD (initial ranging from 200 to 3,000 mg/L), colour (initial ranging from 450 to 2000 ADMI) of sterilised raw textile wastewater with pH from 6.8 to 9.4. Using a sequential anaerobic-aerobic treatment cycle with hydraulic retention time (HRT) of 24 h, maximum removal of colour and COD achieved was 90% and 80%, respectively.
    Matched MeSH terms: Microscopy, Electron, Scanning/methods; Waste Disposal, Fluid/methods; Water Purification/methods*
  10. Patil NN, Mottrie A, Sundaram B, Patel VR
    Urology, 2008 Jul;72(1):47-50; discussion 50.
    PMID: 18384858 DOI: 10.1016/j.urology.2007.12.097
    To report the collective experience of three multinational institutions with the use of robotics to evaluate and treat complex distal ureteral obstruction.
    Matched MeSH terms: Anastomosis, Surgical/methods; Robotics/methods*; Urologic Surgical Procedures/methods*
  11. Adha PR, Chua KH, Mazlyzam AL, Low KC, Aminuddin BS, Ruszymah BH
    Med J Malaysia, 2008 Jul;63 Suppl A:30-1.
    PMID: 19024968
    A major factor limiting survival following extensive thermal injury is insufficient availability of donor sites to provide enough skin for the required grafting procedures. Limitation of autologous grafting promotes the usage of allograft skin substitutes to promote wound healing. Here, we investigated the wound healing potential of allograft single layered tissue engineered skin which comprises of either keratinocytes (SLTES-K) or fibroblast (SLTES-F) with fibrin as the delivery system. Results from gross and microscopic evaluation showed our single layered tissue engineered skin constructed with keratinocytes or fibroblast after gamma radiation with the dosage of 2Gy could serve as allograft for the treatment of skin loss.
    Matched MeSH terms: Transplantation, Homologous/methods; Skin Transplantation/methods; Tissue Engineering/methods
  12. Venkatesh G, Majid MI, Ramanathan S, Mansor SM, Nair NK, Croft SL, et al.
    Biomed Chromatogr, 2008 May;22(5):535-41.
    PMID: 18205140 DOI: 10.1002/bmc.965
    A simple, sensitive and specific reversed-phase high-performance liquid chromatographic method with UV detection at 251 nm was developed for quantitation of buparvaquone (BPQ) in human and rabbit plasma. The method utilizes 250 microL of plasma and sample preparation involves protein precipitation followed by solid-phase extraction. The method was validated on a C18 column with mobile phase consisting of ammonium acetate buffer (0.02 m, pH 3.0) and acetonitrile in the ratio of 18:82 (v/v) at a flow rate of 1.1 mL/min. The calibration curves were linear (correlation coefficient>or=0.998) in the selected range. The method is specific and sensitive with limit of quantitation of 50 ng/mL for BPQ. The validated method was found to be accurate and precise in the working calibration range. Stability studies were carried out at different storage conditions and BPQ was found to be stable. Partial validation studies were carried out using rabbit plasma and intra- and inter-day precision and accuracy were within 7%. This method is simple, reliable and can be routinely used for preclinical pharmacokinetic studies for BPQ.
    Matched MeSH terms: Chromatography, High Pressure Liquid/methods*; Spectrophotometry, Ultraviolet/methods*; Solid Phase Extraction/methods*
  13. Zain JM, Fauzi AM, Aziz AA
    Conf Proc IEEE Eng Med Biol Soc, 2007 10 20;2006:5459-62.
    PMID: 17946306
    Digital watermarking medical images provides security to the images. The purpose of this study was to see whether digitally watermarked images changed clinical diagnoses when assessed by radiologists. We embedded 256 bits watermark to various medical images in the region of non-interest (RONI) and 480K bits in both region of interest (ROI) and RONI. Our results showed that watermarking medical images did not alter clinical diagnoses. In addition, there was no difference in image quality when visually assessed by the medical radiologists. We therefore concluded that digital watermarking medical images were safe in terms of preserving image quality for clinical purposes.
    Matched MeSH terms: Radiography/methods*; Tomography, X-Ray Computed/methods*; Ultrasonography/methods*
  14. Ujang Z, Soedjono E, Salim MR, Shutes RB
    Water Sci Technol, 2005;52(12):243-50.
    PMID: 16477992
    Municipal leachate was treated in an experimental unit of constructed wetlands of subsurface flow type. The parameters studied were organics (BOD and COD), solids and heavy metals (Zn, Ni, Cu, Cr and Pb). Using two types of emergent plants of Scirpus globulosus and Eriocaulon sexangulare, more than 80% removal was achieved for all the parameters. E. sexangulare removed organics and heavy metals better than Scirpus globulosus. A higher concentration of heavy metals in the influent did not change the removal efficiency.
    Matched MeSH terms: Refuse Disposal/methods*; Waste Disposal, Fluid/methods*; Water Purification/methods*
  15. Othman N, Othman NH
    Malays J Pathol, 2012 Dec;34(2):115-21.
    PMID: 23424773 MyJurnal
    The aim of this study was to examine cells (split-sample) that were retained on sampling devices used to collect conventional Pap smears (primary smears) in order to evaluate specimen adequacy and cytological diagnosis of scrapings that are routinely discarded.
    Matched MeSH terms: Mass Screening/methods*; Specimen Handling/methods*; Vaginal Smears/methods*
  16. Nair SR, Tan LK, Mohd Ramli N, Lim SY, Rahmat K, Mohd Nor H
    Eur Radiol, 2013 Jun;23(6):1459-66.
    PMID: 23300042 DOI: 10.1007/s00330-012-2759-9
    OBJECTIVE: To develop a decision tree based on standard magnetic resonance imaging (MRI) and diffusion tensor imaging to differentiate multiple system atrophy (MSA) from Parkinson's disease (PD).

    METHODS: 3-T brain MRI and DTI (diffusion tensor imaging) were performed on 26 PD and 13 MSA patients. Regions of interest (ROIs) were the putamen, substantia nigra, pons, middle cerebellar peduncles (MCP) and cerebellum. Linear, volumetry and DTI (fractional anisotropy and mean diffusivity) were measured. A three-node decision tree was formulated, with design goals being 100 % specificity at node 1, 100 % sensitivity at node 2 and highest combined sensitivity and specificity at node 3.

    RESULTS: Nine parameters (mean width, fractional anisotropy (FA) and mean diffusivity (MD) of MCP; anteroposterior diameter of pons; cerebellar FA and volume; pons and mean putamen volume; mean FA substantia nigra compacta-rostral) showed statistically significant (P < 0.05) differences between MSA and PD with mean MCP width, anteroposterior diameter of pons and mean FA MCP chosen for the decision tree. Threshold values were 14.6 mm, 21.8 mm and 0.55, respectively. Overall performance of the decision tree was 92 % sensitivity, 96 % specificity, 92 % PPV and 96 % NPV. Twelve out of 13 MSA patients were accurately classified.

    CONCLUSION: Formation of the decision tree using these parameters was both descriptive and predictive in differentiating between MSA and PD.

    KEY POINTS: • Parkinson's disease and multiple system atrophy can be distinguished on MR imaging. • Combined conventional MRI and diffusion tensor imaging improves the accuracy of diagnosis. • A decision tree is descriptive and predictive in differentiating between clinical entities. • A decision tree can reliably differentiate Parkinson's disease from multiple system atrophy.

    Matched MeSH terms: Brain Mapping/methods; Magnetic Resonance Imaging/methods*; Diffusion Tensor Imaging/methods
  17. Britton S, Cheng Q, Sutherland CJ, McCarthy JS
    Malar J, 2015;14:335.
    PMID: 26315027 DOI: 10.1186/s12936-015-0848-3
    To detect all malaria infections in elimination settings sensitive, high throughput and field deployable diagnostic tools are required. Loop-mediated isothermal amplification (LAMP) represents a possible field-applicable molecular diagnostic tool. However, current LAMP platforms are limited by their capacity for high throughput.
    Matched MeSH terms: Colorimetry/methods*; Nucleic Acid Amplification Techniques/methods*; High-Throughput Screening Assays/methods*
  18. Kaur H, Ahmad M, Scaria V
    Interdiscip Sci, 2016 Mar;8(1):95-101.
    PMID: 26298582 DOI: 10.1007/s12539-015-0273-x
    There is emergence of multidrug-resistant Salmonella enterica serotype typhi in pandemic proportions throughout the world, and therefore, there is a necessity to speed up the discovery of novel molecules having different modes of action and also less influenced by the resistance formation that would be used as drug for the treatment of salmonellosis particularly typhoid fever. The PhoP regulon is well studied and has now been shown to be a critical regulator of number of gene expressions which are required for intracellular survival of S. enterica and pathophysiology of disease like typhoid. The evident roles of two-component PhoP-/PhoQ-regulated products in salmonella virulence have motivated attempts to target them therapeutically. Although the discovery process of biologically active compounds for the treatment of typhoid relies on hit-finding procedure, using high-throughput screening technology alone is very expensive, as well as time consuming when performed on large scales. With the recent advancement in combinatorial chemistry and contemporary technique for compounds synthesis, there are more and more compounds available which give ample growth of diverse compound library, but the time and endeavor required to screen these unfocused massive and diverse library have been slightly reduced in the past years. Hence, there is demand to improve the high-quality hits and success rate for high-throughput screening that required focused and biased compound library toward the particular target. Therefore, we still need an advantageous and expedient method to prioritize the molecules that will be utilized for biological screens, which saves time and is also inexpensive. In this concept, in silico methods like machine learning are widely applicable technique used to build computational model for high-throughput virtual screens to prioritize molecules for advance study. Furthermore, in computational analysis, we extended our study to identify the common enriched structural entities among the biologically active compound toward finding out the privileged scaffold.
    Matched MeSH terms: Biological Assay/methods*; Computational Biology/methods*; High-Throughput Screening Assays/methods*
  19. Chan CY, Lim CY, Shahnaz Hasan M, Kwan MK
    Eur Spine J, 2016 05;25 Suppl 1:245-50.
    PMID: 27028615 DOI: 10.1007/s00586-016-4538-9
    PURPOSE: Patients with Fontan circulation have increased cardio-respiratory risk during major spinal surgery. We report a case of severe scoliosis with a Cobb angle of 123.3° in a 16-year-old boy with Fontan circulation treated with single stage posterior segmental pedicle screw instrumentation and fusion.

    METHODS: Case report.

    RESULTS: The use pre-operative halo-ring traction for a duration of 6 weeks in this case lead to improvement in cobb angle from 123.3°, kyphotic angle 87.1° to cobb angle of 78.0°, kyphotic angle 57.2° (on bending and stress films). The operation was completed in 150 min, blood loss 1050 ml (25 ml/kg), and cell salvage of 490 ml. He was immediately extubated post correction, but monitored in ICU for a day. Total length of stay was 8 days without any perioperative morbidity or allogeneic blood transfusion. Final post-operative radiograph showed a cobb angle of 44.2°, kyphotic angle 22.8°. Follow up at 27 months showed solid union with no significant loss of correction.

    CONCLUSION: From this case experience, pre-operative halo traction is a useful surgical strategy in patients with Fontan circulation with severe kyposcoliosis to achieve adequate correction without additional osteotomies to minimize the risk of surgical correction.

    Matched MeSH terms: Preoperative Care/methods; Spinal Fusion/methods*; Traction/methods*
  20. Abu Hassan MR, Leong TW, Othman Andu DF, Hat H, Nik Mustapha NR
    Asian Pac J Cancer Prev, 2016;17(2):569-73.
    PMID: 26925645
    BACKGROUND: A colorectal cancer screening program was piloted in two districts of Kedah in 2013. There is scarcity of information on colorectal cancer screening in Malaysia.

    OBJECTIVE: Thus, this research was conducted to evaluate the colorectal cancer screening program in the districts to provide insights intop its efficacy.

    MATERIALS AND METHODS: A cross sectional study was conducted using data on the colorectal cancer screening program in 2013 involving Kota Setar and Kuala Muda districts in Malaysia. We determined the response rate of immunochemical fecal occult blood test (iFOBT), colonoscopy compliance, and detection rates of neoplasia and carcinoma. We also compared the response of FOBT by demographic background.

    RESULTS: The response rate of FOBT for first iFOBT screening was 94.7% while the second iFOBT screening was 90.7%. Participants from Kuala Muda district were 27 times more likely to default while Indians had a 3 times higher risk of default compared to Malays. The colonoscopy compliance was suboptimal among those with positive iFOBT. The most common finding from colonoscopy was hemorrhoids, followed by tubular adenoma. Detection rate of carcinoma and neoplasia for our program was 1.2%.

    CONCLUSIONS: In summary, the response rate of iFOBT was encouraging but the colonoscopy compliance was suboptimal which led to a considerably low detection rate.

    Matched MeSH terms: Colonoscopy/methods*; Hematologic Tests/methods*; Early Detection of Cancer/methods*
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