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Fulltext Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute

Arif MMA, Fauzi MB, Nordin A, Hiraoka Y, Tabata Y, Yunus MHM

Polymers (Basel), 2020 Nov 13;12(11).
PMID: 33202700 DOI: 10.3390/polym12112678

Gelatin possesses biological properties that resemble native skin and can potentially be fabricated as a skin substitute for full-thickness wound treatment. The native property of gelatin, whereby it is easily melted and degraded at body temperature, could prevent its biofunctionality for various applications. This study aimed to fabricate and characterise buffalo gelatin (Infanca halal certified) crosslinked with chemical type crosslinker (genipin and genipin fortified with EDC) and physicaly crosslink using the dihydrothermal (DHT) method. A porous gelatin sponge (GS) was fabricated by a freeze-drying process followed by a complete crosslinking via chemical-natural and synthetic-or physical intervention using genipin (GNP), 1-ethyl-3-(3-dimethylaminopropyl) (EDC) and dihydrothermal (DHT) methods, respectively. The physicochemical, biomechanical, cellular biocompatibility and cell-biomaterial interaction of GS towards human epidermal keratinocytes (HEK) and dermal fibroblasts (HDF) were evaluated. Results showed that GS had a uniform porous structure with pore size ranging between 60 and 200 µm with high porosity (>78.6 ± 4.1%), high wettability (<72.2 ± 7.0°), high tensile strain (>13.65 ± 1.10%) and 14 h of degradation rate. An increase in the concentration and double-crosslinking approach demonstrated an increment in the crosslinking degree, enzymatic hydrolysis resistance, thermal stability, porosity, wettability and mechanical strength. The GS can be tuned differently from the control by approaching the GS via a different crosslinking strategy. However, a decreasing trend was observed in the pore size, water retention and water absorption ability. Crosslinking with DHT resulted in large pore sizes (85-300 µm) and low water retention (236.9 ± 18.7 g/m2·day) and a comparable swelling ratio with the control (89.6 ± 7.1%). Moreover no changes in the chemical content and amorphous phase identification were observed. The HEK and HDF revealed slight toxicity with double crosslinking. HEK and HDF attachment and proliferation remain similar to each crosslinking approach. Immunogenicity was observed to be higher in the double-crosslinking compared to the single-crosslinking intervention. The fabricated GS demonstrated a dynamic potential to be tailored according to wound types by manipulating the crosslinking intervention.
Fulltext Possible Participation of Ionotropic Glutamate Receptors and l-Arginine-Nitric Oxide-Cyclic Guanosine Monophosphate-ATP-Sensitive K+ Channel Pathway in the Antinociceptive Activity of Cardamonin in Acute Pain Animal Models

Pui Ping C, Akhtar MN, Israf DA, Perimal EK, Sulaiman MR

Molecules, 2020 Nov 18;25(22).
PMID: 33217904 DOI: 10.3390/molecules25225385

The perception of pain caused by inflammation serves as a warning sign to avoid further injury. The generation and transmission of pain impulses involves various pathways and receptors. Cardamonin isolated from Boesenbergia rotunda (L.) Mansf. has been reported to exert antinociceptive effects in thermal and mechanical pain models; however, the precise mechanism has yet to be examined. The present study investigated the possible mechanisms involved in the antinociceptive activity of cardamonin on protein kinase C, N-methyl-d-aspartate (NMDA) and non-NMDA glutamate receptors, l-arginine/cyclic guanosine monophosphate (cGMP) mechanism, as well as the ATP-sensitive potassium (K+) channel. Cardamonin was administered to the animals intra-peritoneally. Present findings showed that cardamonin significantly inhibited pain elicited by intraplantar injection of phorbol 12-myristate 13-acetate (PMA, a protein kinase C activator) with calculated mean ED50 of 2.0 mg/kg (0.9-4.5 mg/kg). The study presented that pre-treatment with MK-801 (NMDA receptor antagonist) and NBQX (non-NMDA receptor antagonist) significantly modulates the antinociceptive activity of cardamonin at 3 mg/kg when tested with glutamate-induced paw licking test. Pre-treatment with l-arginine (a nitric oxide precursor), ODQ (selective inhibitor of soluble guanylyl cyclase) and glibenclamide (ATP-sensitive K+ channel inhibitor) significantly enhanced the antinociception produced by cardamonin. In conclusion, the present findings showed that the antinociceptive activity of cardamonin might involve the modulation of PKC activity, NMDA and non-NMDA glutamate receptors, l-arginine/nitric oxide/cGMP pathway and ATP-sensitive K+ channel.
Fulltext Mechanistic Approaches of Internalization, Subcellular Trafficking, and Cytotoxicity of Nanoparticles for Targeting the Small Intestine

Madni A, Rehman S, Sultan H, Khan MM, Ahmad F, Raza MR, et al.

AAPS PharmSciTech, 2020 Nov 22;22(1):3.
PMID: 33221968 DOI: 10.1208/s12249-020-01873-z

Targeting the small intestine employing nanotechnology has proved to be a more effective way for site-specific drug delivery. The drug targeting to the small intestine can be achieved via nanoparticles for its optimum bioavailability within the systemic circulation. The small intestine is a remarkable candidate for localized drug delivery. The intestine has its unique properties. It has a less harsh environment than the stomach, provides comparatively more retention time, and possesses a greater surface area than other parts of the gastrointestinal tract. This review focuses on elaborating the intestinal barriers and approaches to overcome these barriers for internalizing nanoparticles and adopting different cellular trafficking pathways. We have discussed various factors that contribute to nanocarriers' cellular uptake, including their surface chemistry, surface morphology, and functionalization of nanoparticles. Furthermore, the fate of nanoparticles after their uptake at cellular and subcellular levels is also briefly explained. Finally, we have delineated the strategies that are adopted to determine the cytotoxicity of nanoparticles.
Photoluminescence Investigation of Carrier Localization in Colloidal PbS and PbS/MnS Quantum Dots

Zaini MS, Liew JYC, Alang Ahmad SA, Mohmad AR, Ahmad Kamarudin M

ACS Omega, 2020 Dec 08;5(48):30956-30962.
PMID: 33324803 DOI: 10.1021/acsomega.0c03768

The existence of surface organic capping ligands on quantum dots (QDs) has limited the potential in QDs emission properties and energy band gap structure alteration as well as the carrier localization. This drawback can be addressed via depositing a thin layer of a semiconductor material on the surface of QDs. Herein, we report on the comparative study for photoluminescent (PL) properties of PbS and PbS/MnS QDs. The carrier localization effect due to the alteration of energy band gap structure and carrier recombination mechanism in the QDs were investigated via PL measurements in a temperature range of 10-300 K with the variation of the excitation power from 10 to 200 mW. For PbS QDs, the gradient of integrated PL intensity (IPL) as a function of excitation power density graph was less than unity. When the MnS shell layer was deposited onto the PbS core, the PL emission exhibited a blue shift, showing dominant carrier recombination. It was also found that the full width half-maximum showed a gradual broadening with the increasing temperature, affirming the electron-phonon interaction.
Fulltext Evaluation of Patient's Knowledge, Attitude, and Practice of Cross-Infection Control in Dentistry during COVID-19 Pandemic

Ahmed MA, Jouhar R, Adnan S, Ahmed N, Ghazal T, Adanir N

Eur J Dent, 2020 Dec;14(S 01):S1-S6.
PMID: 33321543 DOI: 10.1055/s-0040-1721295

OBJECTIVES: The aim of this study was to evaluate the patient's knowledge, attitude, and practice of cross-infection control in dentistry during coronavirus diseases 2019 (COVID-19).
MATERIALS AND METHODS: This cross-sectional observational study was conducted at Altamash Institute of Dental Medicine, Karachi from June 2020 to August 2020. A total of 775 patients participated to fill questionnaire. After scrutiny, 25 questionnaire forms were excluded due to incomplete information. Questionnaire was divided in to four sections. First section obtained patients sociodemographic information, while knowledge of patients regarding cross-infection control during COVID-19 pandemic was evaluated in second section. Similarly, the purpose of third section was to assess attitude and fourth section was designed to evaluate practice. Likert's 3-point scale was used for registration of participant's response. The reliability of questionnaire was assessed through Crohnbach's α (α = 0.74). Statistical analysis was performed after entering data in to SPSS version 25. Independent t-test was used to evaluate the difference in patients' knowledge, attitude, and practice. A p-value ≤ 0.05 was considered as statistically significant.
RESULTS: Majority of the participants agreed that COVID-19 is a highly contagious disease (631, 97%) and daily screening of staff, dental assistants, dentists, and patients is necessary (568, 75.7%) along with proper sterilization of instruments and disinfection of dental operatory (650, 86.66%). A large number of participant also agreed that proper disposal of waste is utmost important for cross-infection control (601, 80.24%) and patient's waiting area should also be marked with social distancing sign (620, 82.66%). However, when asked about the common route of COVID-19 transmission in dentistry, 341 (45.5%) participants disagreed that it is not through aerosol (water droplets) generated during dental procedures.
CONCLUSIONS: Dental health professionals should take additional measures to educate dental patients on mechanism of potential spread of COVID-19 through the aerosols and droplets generated during dental procedures. They should also encourage the dental patients to be vigilant and question the cross-infection control measures practiced by the dentist to whom they go for dental treatment, and to decrease their chances of acquiring an infection during their visit to the dental office.
Fulltext Focused surgical pandemic response in a Malaysian hybrid COVID-19 hospital

Roslani AC, Vythilingam G, Seevalingam KK, Xavier RG, Idris MS, Karuppiah R

Asian J Surg, 2021 Jan;44(1):404-406.
PMID: 33317901 DOI: 10.1016/j.asjsur.2020.10.012
Fulltext Effect of nanoparticles concentration on electromagnetic-assisted oil recovery using ZnO nanofluids

Adil M, Lee K, Mohd Zaid H, A Shukur MF, Manaka T

PLoS One, 2020;15(12):e0244738.
PMID: 33382855 DOI: 10.1371/journal.pone.0244738

Utilization of metal-oxide nanoparticles (NPs) in enhanced oil recovery (EOR) has generated substantial recent research interest in this area. Among these NPs, zinc oxide nanoparticles (ZnO-NPs) have demonstrated promising results in improving oil recovery due to their prominent thermal properties. These nanoparticles can also be polarized by electromagnetic (EM) field, which offers a unique Nano-EOR approach called EM-assisted Nano-EOR. However, the impact of NPs concentrations on oil recovery mechanism under EM field has not been well established. For this purpose, ZnO nanofluids (ZnO-NFs) of two different particle sizes (55.7 and 117.1 nm) were formed by dispersing NPs between 0.01 wt.% to 0.1 wt.% in a basefluid of sodium dodecylbenzenesulfonate (SDBS) and NaCl to study their effect on oil recovery mechanism under the electromagnetic field. This mechanism involved parameters, including mobility ratio, interfacial tension (IFT) and wettability. The displacement tests were conducted in water-wet sandpacks at 95˚C, by employing crude oil from Tapis. Three tertiary recovery scenarios have been performed, including (i) SDBS surfactant flooding as a reference, (ii) ZnO-NFs flooding, and (iii) EM-assisted ZnO-NFs flooding. Compare with incremental oil recovery from surfactant flooding (2.1% original oil in place/OOIP), nanofluid flooding reaches up to 10.2% of OOIP at optimal 0.1 wt.% ZnO (55.7 nm). Meanwhile, EM-assisted nanofluid flooding at 0.1 wt.% ZnO provides a maximum oil recovery of 10.39% and 13.08% of OOIP under EM frequency of 18.8 and 167 MHz, respectively. By assessing the IFT/contact angle and mobility ratio, the optimal NPs concentration to achieve a favorable ER effect and interfacial disturbance is determined, correlated to smaller hydrodynamic-sized nanoparticles that cause strong electrostatic repulsion between particles.
Fulltext Sequence and phylogeny of the complete mitochondrial genome of the Himalayan jungle crow (Corvidae: Corvus macrorhynchos intermedius) from Pakistan

Iqbal F, Ayub Q, Song BK, Wilson R, Fahim M, Rahman S

Mitochondrial DNA B Resour, 2019 Dec 18;5(1):348-350.
PMID: 33366551 DOI: 10.1080/23802359.2019.1704637

Corvus macrorhynchos formerly referred to as the jungle crow or the large-billed crow is a polytypic species with unresolved taxonomy, comprising various subspecies widespread across South, Southeast, and East Asia. In this study, we report the complete mitogenome of one of these subspecies, Corvus macrorhynchos intermedius (Himalaya crow), from Pakistan. The mitochondrial genome is circular, 16,927 bp and contains typical animal mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNA) and one non-coding region (D-loop) with a nucleotide content of A (30.6%), T (24.8%), G (14.8%), and C (29.8%). Phylogenetic analysis using the whole mitochondrial genome showed that C. m. intermedius and only reported subspecies Corvus macrorhynchos culminatus (Indian Jungle crow) are genetically distinct and it supports the recognition of the latter as a separate biospecies.
Fulltext A novel method for the quantification of industrial and municipal waste materials for environmental hazard assessment

Arifin MH, Kayode JS, Ismail MKI, Abdullah AM, Embrandiri A, Nazer NSM, et al.

MethodsX, 2021;8:101182.
PMID: 33365262 DOI: 10.1016/j.mex.2020.101182

A novel methodological approach was developed to quantified the volume of industrial waste desposal (IWD) site, combined with municipal waste materials (MWM), through the integration of a non-invasive, fast, and less expenssive RES2-D Electrical Resistivity Technique (ERT), using Wenner-Schlumberger electrode array geophysical method with Oasis Montaj software. Underground water bearing structures, and the eco-system are being contaminated through seepage of the plumes emanating from the mixtures of the industrial waste materials (IWM), made of moist cemented soil with municipal solid wastes (MSW) dumped at the site. The distribution of the contiminant hazardous plumes emanating from the waste materials' mixtures within the subsurface structural lithological layers was clearly map and delineated within the near-surface structures, using the triplicate technique to collect samples of the soil with the waste mixtures, and the water analysis for the presence of dissolved ions. The deployed method helped to monitor the seepage of the contaminant leachate plumes to the groundwater aquifer units via the ground surface, through the subsurface stratum lithological layers, and hence, estimation of the waste materials' volume was possibly approximated to be 312,000 m3. In summary, the novel method adopted are as presented below:•The novel method is transferable, reproduce-able, and most importantly, it is unambiguous technique for the quantification of environmental, industrial and municipal waste materials.•It helps to map the distribution of the plumes emanating from the waste materials' mixtures within the subsurface structural lithological layers that was clearly delineated within the near-surface structures underlain the study site.•The procedure helped in the monitoring of leachate contaminants plumes seepages into the surface water bodies and the groundwater aquifer units, via the ground surface, through to the porous subsurface stratum lithological layers.
Fulltext Demographic and socioeconomic inequalities in oral healthcare utilisation in Malaysia: evidence from a national survey

Tan YR, Tan EH, Jawahir S, Mohd Hanafiah AN, Mohd Yunos MH

BMC Oral Health, 2021 01 19;21(1):34.
PMID: 33468125 DOI: 10.1186/s12903-020-01388-w

BACKGROUND: Throughout the years, oral healthcare utilisation in Malaysia has been low despite various efforts by the Ministry of Health Malaysia for improvement. This study aimed to determine the prevalence of oral healthcare utilisation and identify factors associated with oral healthcare utilisation among adults in Malaysia.
METHODS: Secondary data analysis of adults aged 18 years and over from the National Health and Morbidity Survey 2019 was conducted in this study. Characteristics of respondents and those who utilised oral healthcare were described using complex sample descriptive statistics. Logistic regression analysis was performed to examine the association between the dependent and independent variables. Dependent variable was oral healthcare utilisation in the last 12 months. Independent variables were demographic and socioeconomic factors (predisposing, enabling and need characteristics) based on Andersen's Behavioural Model.
RESULTS: A total of 11,308 respondents, estimated to represent 21.7 million adults aged 18 years and over in Malaysia were included in the analysis. Prevalence of oral healthcare utilisation in the last 12 months was 13.2%. Demographic factors of sex, age, marital status, and socioeconomic factors of education level and occupation as well as health belief such as medical check-up were significantly related to oral healthcare utilisation. Enabling factor of household income quintile had significant association with oral healthcare utilisation. Inequalities were observed; females (OR = 1.57, 95% CI = 1.25, 1.96), younger adults (OR = 1.64, 95% CI = 1.15, 2.33), those who were married (OR = 1.65, 95% CI = 1.23, 2.22), those with higher education (OR = 2.21, 95% CI = 1.23, 3.99), those who had medical check-up in the last 12 months (OR = 1.86, 95% CI = 1.53, 2.25) and those with higher income (OR = 1.43, 95% CI = 1.04, 1.96) were more likely to utilise oral healthcare.
CONCLUSION: Understanding factors associated with utilisation of oral healthcare could help in formulating effective interventions to improve oral healthcare utilisation. Demographic and socioeconomic factors are strong determinants of oral healthcare utilisation in Malaysia. Appropriate interventions to strengthen the existing programmes aimed to promote regular and timely oral health check-ups are needed to improve oral healthcare utilisation.
Does tradeoff between financial and social indicators matters in environmental consideration: evidence from G7 region

Van Song N, Phuong NTM, Oanh TTK, Chien DH, Phuc VQ, Mohsin M

Environ Sci Pollut Res Int, 2021 Apr;28(16):19911-19925.
PMID: 33410000 DOI: 10.1007/s11356-020-12041-y

The study tries to discover the impact of financial and social indicators' growth towards environmental considerations to understand the drivers of economic growth and carbon dioxide emissions change in G7 countries. The DEA-like composite index has been used to examine the tradeoff between financial and social indicator matters in environmental consideration by using a multi-objective goal programming approach. The data from 2008 to 2018 is collected from G-7 countries. The results from the DEA-like composite index reveals that there is a mixed condition of environmental sustainability in G-7 countries where the USA is performing better and Japan is performing worse among the set of other countries. The further result shows that the energy and fiscal indicators help to decrease the dangerous gas emissions. Divergent to that, the human and financial index positively contributes to greenhouse gas emissions. Fostering sustainable development is essential to successfully reduce emissions, meet established objectives, and ensure steady development. The study provides valuable information for policymakers.
Fulltext Isolated Pancreatic Tail Injury in Paediatrics; A Case Report and Literature Review

Al Kuddoos NM, Ismail AK, Shen KWC, Shibraumalisi NA, Yunus AY

Bull Emerg Trauma, 2020 Oct;8(4):249-252.
PMID: 33426141 DOI: 10.30476/beat.2020.85719

Pancreatic injury in paediatrics is a rare condition and can be difficult to diagnose. The diagnostic challenge is due to its symptom of vague abdominal pain which usually results in late presentation. Elevated biochemical markers such as amylase and lipase can aid in the evaluation of pancreatic injury, however, it is not specific and is only evident after several hours of trauma. Ultrasound is commonly used as a primary modality to evaluate abdominal organ injuries, but its role in detecting pancreatic injury is limited due to low sensitivity. High index of suspicion is needed to avoid undiagnosed pancreatic injury which could be lethal to children. We herein report a case of delayed presentation of isolated pancreatic tail injury in a child who was sufficiently diagnosed with ultrasound and treated conservatively. Proper initial assessment and diagnosis will allow appropriate management of pancreatic injury. Conservative management should include serial imaging to look at the evolution of pancreatic injury and detect complications such as pseudocyst or abscess formation.
The step towards environmental mitigation in Pakistan: do transportation services, urbanization, and financial development matter?

Godil DI, Ahmad P, Ashraf MS, Sarwat S, Sharif A, Shabib-Ul-Hasan S, et al.

Environ Sci Pollut Res Int, 2021 May;28(17):21486-21498.
PMID: 33415625 DOI: 10.1007/s11356-020-11839-0

This study is a scholarly effort to broaden the existing literature on the impact of transportation services, urbanization, and financial development on ecological footprints in Pakistan. Data used in this study covers the period of 39 years from 1980 to 2018. This study adopted the QARDL model to tackle the non-linear association of variables and test their long-run stability across the different quantiles. The findings of this study indicated a significant negative association of transportation services and financial development with ecological footprints in Pakistan at almost all quantiles whereas, the urban population was found to be positively associated with the ecological footprint in Pakistan. Results also justify the existence of the EKC hypothesis in the scenario of Pakistan. Policymakers are advised to frame strategies for investors to invest more in eco-friendly projects to curtail the ecological footprints in Pakistan. Minimizing the dependency of the transportation sector on fossil fuel, and increased use of energy-efficient appliances in the urban population would be beneficial to control the negative influence on ecological footprints in Pakistan.
Fulltext Knowledge, attitude and perceptions about Crimean Congo Haemorrhagic Fever (CCHF) among occupationally high-risk healthcare professionals of Pakistan

Ahmed A, Saqlain M, Tanveer M, Tahir AH, Ud-Din F, Shinwari MI, et al.

BMC Infect Dis, 2021 Jan 07;21(1):35.
PMID: 33413164 DOI: 10.1186/s12879-020-05714-z

BACKGROUND: Crimean Congo Haemorrhagic Fever (CCHF), a tropically neglected infectious disease caused by Nairovirus, is endemic in low middle-income countries like Pakistan. Emergency health care professionals (HCPs) are at risk of contracting nosocomial transmission of CCHF. We, therefore, aim to analyze the knowledge, attitudes, and perceptions (KAP) of at-risk physicians, nurses, and pharmacists in Pakistan and the factors associated with good KAP.
METHOD: A validated questionnaire (Cronbach's alpha 0.71) was used to collect data from HCPs in two CCHF endemic metropolitan cities of Pakistan by employing a cross-sectional study design. For data analysis percentages, chi-square test and Spearman correlation were applied by using SPSS version 22.
RESULTS: Of the 478 participants, 56% (n = 268) were physicians, 37.4% (n = 179) were nurses, and 6.5% (n = 31) were pharmacists. The proportion of HCPs with good knowledge, attitude, and perception scores was 54.3%, 81, and 69%, respectively. Being a physician, having more work experience, having a higher age, working in tertiary care settings, were key factors for higher knowledge (p
Plant growth promoting and antifungal asset of indigenous rhizobacteria secluded from saffron (Crocus sativus L.) rhizosphere

Rasool A, Imran Mir M, Zulfajri M, Hanafiah MM, Azeem Unnisa S, Mahboob M

Microb Pathog, 2021 Jan;150:104734.
PMID: 33429050 DOI: 10.1016/j.micpath.2021.104734

Saffron (Crocus sativus L.) is an important plant in medicine. The Kashmir Valley (J&K, India) is one of the world's largest and finest saffron producing regions. However, over the past decade, there has been a strong declining trend in saffron production in this area. Plant Growth Promoting Rhizobacteria (PGPR) are free living soil bacteria that have ability to colonize the surfaces of the roots and ability to boost plant growth and development either directly or indirectly. Using the efficient PGPR as a bio-inoculant is another sustainable agricultural practice to improve soil health, grain yield quality, and biodiversity conservation. In the present study, a total of 13 bacterial strains were isolated from rhizospheric soil of saffron during the flowering stage of the tubers and were evaluated for various plant growth promoting characteristics under in vitro conditions such as the solubilization of phosphate, production of indole acetic acid, siderophore, hydrocyanic acid, and ammonia production and antagonism by dual culture test against Sclerotium rolfsii and Fusarium oxysporum. All the isolates were further tested for the production of hydrolytic enzymes such as protease, lipase, amylase, cellulase, and chitinase. The maximum proportions of bacterial isolates were gram-negative bacilli. About 77% of the bacterial isolates showed IAA production, 46% exhibited phosphate solubilization, 46% siderophore, 61% HCN, 100% ammonia production, 69% isolates showed protease activity, 62% lipase, 46% amylase, 85% cellulase, and 39% showed chitinase activity. Three isolates viz., AIS-3, AIS-8 and AIS-10 were found to have the most plant growth properties and effectively control the growth of Sclerotium rolfsii and Fusarium oxysporum. The bacterial isolates were identified as Brevibacterium frigoritolerans (AIS-3), Alcaligenes faecalis subsp. Phenolicus (AIS-8) and Bacillus aryabhattai (AIS-10) respectively by 16S rRNA sequence analysis. Therefore, these isolated rhizobacterial strains could be a promising source of plant growth stimulants to increase cormlets growth and increase saffron production.
Perspectives of community pharmacists in Pakistan about practice change and implementation of extended pharmacy services: a mixed method study

Hashmi F, Hassali MA, Saleem F, Saeed H, Islam M, Malik UR, et al.

Int J Clin Pharm, 2021 Aug;43(4):1090-1100.
PMID: 33411180 DOI: 10.1007/s11096-020-01221-y

Background Community pharmacists' role in the primary health care, patient-centered services, beyond traditional dispensing services is well established in the developed world. However, this role is not fully established in low-middle-income countries including Pakistan. Objective To explore community pharmacists' perspectives and preparedness about practice change and associated facilitators and barriers to extended services. Setting A study was conducted involving community pharmacists of Lahore, Pakistan. Method Two phased studies were conducted using mixed-method sequential design. The first phase involved qualitative semi-structured face-to-face interviews with the community pharmacists, while the second phase constituted a questionnaire-based cross-sectional study. Main outcome measure Pharmacists' perspectives about extended pharmacy services, facilitators, barriers and preparedness for the practice change. Results For the first phase, pharmacists were purposively sampled and the saturation yielded a final sample size of fifteen pharmacists (N = 15). The thematic content analysis yielded four distinct themes; (1) current practices and familiarity with extended pharmacy services (2) practice gap between Pakistan and the developed countries (3) facilitators and the preparedness, and (4) barriers towards its implementation and provision. The second part was a questionnaire-based cross-sectional phase, where a total of 348 community pharmacists were approached, while only 242 responded, yielding a response rate of 69.5%. The triangulation of qualitative and quantitative data identified several barriers such as; shortage of pharmacists, lack of knowledge and skills, poor collaboration with general practitioners, failure of customers to pay for extended services. Facilitators and preparedness for the provision of extended pharmacy services were; access to patient notes, follow-up, separate counselling areas, accreditation of specific services and sufficient resources. Conclusion This study's findings call for the implementation and enforcement of Punjab Drug Sale Rules 2007 to facilitate the practice change and provision of comprehensive pharmacy services in Punjab, Pakistan. There is a need for additional laws to define community pharmacists' roles, uniform job description, training, new funding model, separate area, and accreditation of extended pharmacy services in Pakistan.
Fulltext Comprehensive computational target fishing approach to identify Xanthorrhizol putative targets

Shahid M, Azfaralariff A, Law D, Najm AA, Sanusi SA, Lim SJ, et al.

Sci Rep, 2021 01 15;11(1):1594.
PMID: 33452398 DOI: 10.1038/s41598-021-81026-9

Xanthorrhizol (XNT), is a bioactive compound found in Curcuma xanthorrhiza Roxb. This study aimed to determine the potential targets of the XNT via computational target fishing method. This compound obeyed Lipinski's and Veber's rules where it has a molecular weight (MW) of 218.37 gmol-1, TPSA of 20.23, rotatable bonds (RBN) of 4, hydrogen acceptor and donor ability is 1 respectively. Besides, it also has half-life (HL) values 3.5 h, drug-likeness (DL) value of 0.07, oral bioavailability (OB) of 32.10, and blood-brain barrier permeability (BBB) value of 1.64 indicating its potential as therapeutic drug. Further, 20 potential targets were screened out through PharmMapper and DRAR-CPI servers. Co-expression results derived from GeneMANIA revealed that these targets made connection with a total of 40 genes and have 744 different links. Four genes which were RXRA, RBP4, HSD11B1 and AKR1C1 showed remarkable co-expression and predominantly involved in steroid metabolic process. Furthermore, among these 20 genes, 13 highly expressed genes associated with xenobiotics by cytochrome P450, chemical carcinogenesis and steroid metabolic pathways were identified through gene ontology (GO) and KEGG pathway analysis. In conclusion, XNT is targeting multiple proteins and pathways which may be exploited to shape a network that exerts systematic pharmacological effects.
Fulltext Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116

Samad MA, Saiman MZ, Abdul Majid N, Karsani SA, Yaacob JS

Molecules, 2021 Jan 13;26(2).
PMID: 33450878 DOI: 10.3390/molecules26020376

Colorectal cancer (CRC) is the most common cancer among males and females, which is associated with the increment of telomerase level and activity. Some plant-derived compounds are telomerase inhibitors that have the potential to decrease telomerase activity and/or level in various cancer cell lines. Unfortunately, a deeper understanding of the effects of telomerase inhibitor compound(s) on CRC cells is still lacking. Therefore, in this study, the aspects of telomerase inhibitors on a CRC cell line (HCT 116) were investigated. Screening on HCT 116 at 48 h showed that berberine (10.30 ± 0.89 µg/mL) is the most effective (lowest IC50 value) telomerase inhibitor compared to boldine (37.87 ± 3.12 µg/mL) and silymarin (>200 µg/mL). Further analyses exhibited that berberine treatment caused G0/G1 phase arrest at 48 h due to high cyclin D1 (CCND1) and low cyclin-dependent kinase 4 (CDK4) protein and mRNA levels, simultaneous downregulation of human telomerase reverse transcriptase (TERT) mRNA and human telomerase RNA component (TERC) levels, as well as a decrease in the TERT protein level and telomerase activity. The effect of berberine treatment on the cell cycle was time dependent as it resulted in a delayed cell cycle and doubling time by 2.18-fold. Telomerase activity and level was significantly decreased, and telomere erosion followed suit. In summary, our findings suggested that berberine could decrease telomerase activity and level of HCT 116, which in turn inhibits the proliferative ability of the cells.
Fulltext The Influence of Ficus deltoidea in Preserving Alveolar Bone in Ovariectomized Rats

Omar NI, Baharin B, Lau SF, Ibrahim N, Mohd N, Ahmad Fauzi A, et al.

Vet Med Int, 2020;2020:8862489.
PMID: 33456747 DOI: 10.1155/2020/8862489

Ficus deltoidea has been shown to possess antioxidant properties that could prevent the development of chronic inflammatory bone diseases. In this study, the efficacy of F. deltoidea in preventing alveolar bone resorption in osteoporotic rats induced by ovariectomy (OVX) was investigated. Twenty-four female Wistar rats were divided into four groups (n = 6) consisting of sham-operated (SO), ovariectomized control (OVXN), ovariectomized treated with estrogen (OVXP), and ovariectomized treated with F. deltoidea extract (OVXF). At the beginning of the study, two nonovariectomized, healthy rats were sacrificed to serve as baseline (BL). Treatment of the rats commenced two weeks after ovariectomy-the OVXP rats that served as positive control received Premarin® (64.5 μg/kg body weight), while OVXF rats were given F. deltoidea (800 mg/kg body weight); both agents were administered orally for two months. The negative control group of rats (OVXN) and the SO group received deionized water, also administered via oral gavage. At necropsy, morphometric assessment of the interradicular bone of the first molar was carried out using a micro-CT scanner, while quantification of osteoclasts and osteoblasts was performed histologically. The results showed that no statistically significant differences among the groups (p > 0.05) for bone morphometric assessment. However, trabecular thickness in the OVXF group was similar to BL, while trabecular separation and alveolar bone loss height were lower than those of the OVXN group. Histologically, the OVXF group demonstrated a significantly lower number of osteoclasts and a higher number of osteoblasts compared with OVXN (p=0.008 and p=0.019, respectively; p < 0.05). In conclusion, F. deltoidea has the capacity to prevent alveolar bone loss in ovariectomy-induced osteoporosis rats by potentially preserving trabecular bone microarchitecture and to decrease osteoclast and increase osteoblast cell count.
Fulltext Oxidative Desulfurization of Petroleum Distillate Fractions Using Manganese Dioxide Supported on Magnetic Reduced Graphene Oxide as Catalyst

Ahmad W, Ur Rahman A, Ahmad I, Yaseen M, Mohamed Jan B, Stylianakis MM, et al.

Nanomaterials (Basel), 2021 Jan 14;11(1).
PMID: 33466855 DOI: 10.3390/nano11010203

In this study, oxidative desulfurization (ODS) of modeled and real oil samples was investigated using manganese-dioxide-supported, magnetic-reduced graphene oxide nanocomposite (MnO2/MrGO) as a catalyst in the presence of an H2O2/HCOOH oxidation system. MnO2/MrGO composite was synthesized and characterized by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) analyses. The optimal conditions for maximum removal of dibenzothiophene (DBT) from modeled oil samples were found to be efficient at 40 °C temperature, 60 min reaction time, 0.08 g catalyst dose/10 mL, and 2 mL of H2O2/formic acid, under which MnO2/MrGO exhibited intense desulfurization activity of up to 80%. Under the same set of conditions, the removal of only 41% DBT was observed in the presence of graphene oxide (GO) as the catalyst, which clearly indicated the advantage of MrGO in the composite catalyst. Under optimized conditions, sulfur removal in real oil samples, including diesel oil, gasoline, and kerosene, was found to be 67.8%, 59.5%, and 51.9%, respectively. The present approach is credited to cost-effectiveness, environmental benignity, and ease of preparation, envisioning great prospects for desulfurization of fuel oils on a commercial level.

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