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Fulltext Antimicrobial drug resistance of Staphylococcus aureus in dairy products

Sasidharan S, Prema B, Yoga LL

Asian Pac J Trop Biomed, 2011 Apr;1(2):130-2.
PMID: 23569742 DOI: 10.1016/S2221-1691(11)60010-5

To evaluate the prevalence of multidrug resistant Staphylococcus aureus (S. aureus) in dairy products.

Matched MeSH terms: Dairy Products/microbiology*
Nutrient Intake in Vietnamese Preschool and School-Aged Children is Not Adequate: The Role of Dairy

Bao Khanh le N, Burgers MR, Huu Chinh N, Tuoc BV, Dinh Dung N, Deurenberg P, et al.

Food Nutr Bull, 2016 Mar;37(1):100-11.
PMID: 27004970 DOI: 10.1177/0379572116631642

The traditional Vietnamese diet carries the risk of micronutrient deficiencies, and a substantial part of children <11 years do not meet the Vietnamese recommended dietary allowance (RDA) for a range of nutrients. Dairy products are known for their high nutrient density and milk in particular for its provision of high-quality protein and relevant concentrations of calcium, magnesium, vitamin B2, vitamin B12, and pantothenic acid.

Matched MeSH terms: Dairy Products*
Conjugated linoleic acid: A potent fatty acid linked to animal and human health

Shokryzadan P, Rajion MA, Meng GY, Boo LJ, Ebrahimi M, Royan M, et al.

Crit Rev Food Sci Nutr, 2017 Sep 02;57(13):2737-2748.
PMID: 26252346 DOI: 10.1080/10408398.2015.1060190

Conjugated linoleic acid (CLA) is a mixture of isomers of linoleic acid (C18:2 n-6), which is mostly found in the ruminant meat and dairy products. The CLA is known to have many potential health benefits, and considered a potent powerful fatty acid, which is linked to animal and human health. The present work aims to discuss the source and production, mechanism of action, and effects of CLA on humans, poultry, and ruminants by reviewing the recent studies carried out on CLA. Despite most of the recent studies indicating beneficial effects of CLA on improving body weight control parameters, its effects on reducing risk factors of cardiovascular diseases (CVD), inflammation, blood glucose, and insulin are still controversial, and need to be further studied in different hosts.

Matched MeSH terms: Dairy Products/analysis*
Dairy intake and type 2 diabetes risk factors: A narrative review

Mitri J, Mohd Yusof BN, Maryniuk M, Schrager C, Hamdy O, Salsberg V

Diabetes Metab Syndr, 2019 08 01;13(5):2879-2887.
PMID: 31425952 DOI: 10.1016/j.dsx.2019.07.064

AIM: The interest regarding the potential role of dairy products in the prevention of type 2 diabetes (T2D) has emerged. Although results remain mixed, numerous cohort studies have shown that increased dairy consumption is inversely associated with T2D risk. This narrative review evaluates the recent evidence of dairy products intake on T2D risk factors for the prevention of T2D.
MATERIAL AND METHOD: The review is framed within the systematic review and meta-analyses of cohort studies and the individual randomized controlled trials evidence. We searched for existing meta-analyses of cohort studies that addressed the association of dairy intake with incidence of T2D in adults using the MEDLINE (via PubMed) database. For the interventional studies, the literature searched was conducted using MEDLINE (via PubMed) with the following Medical Subjects Heading (MeSH) terms i.e. dairy OR milk OR cheese OR yogurt AND glucose OR diabetes OR insulin resistance OR insulin sensitivity OR pre-diabetes.
RESULTS: Most of the meta-analyses and systematic reviews of the cohort studies point to a reduced risk of T2D with dairy intake of 3 servings per day. This effect was mainly attributed to low-fat dairy, particularly yogurt and cheese. However, there is no evidence in cohort studies that high-fat dairy intake poses any harm.
CONCLUSION: Dairy products, when incorporated into a healthy diet, likely do not have detrimental effects on glucose-related outcomes. The potential impact of dairy consumption on glucose tolerance tests, insulin levels, insulin sensitivity measures, and plasma glucose levels warrant future investigation.

Matched MeSH terms: Dairy Products/statistics & numerical data*
The thiamine content of human milk in Malaya. I. The normal level of thiamine in milk from Malay, Chinese and Indian women

Simpson IA, Chow AY

J Trop Pediatr (Lond), 1956 Jun;2(1):3-17.
PMID: 24544028 DOI: 10.1093/oxfordjournals.tropej.a057405

(1) The use of the thiochrome method for the estimation of thiamine in human milk is discussed, with special reference to the possible production of an artefact, following the incubation of the milk with pepsin, in addition to the use of takadiastase (clarase). No evidence could be found to suggest that an artefact resembling thiamine was produced by the method of analysis described.
(2) The thiamine content of 91 samples of full lactation human milk from apparently "normal" Malay, Chinese and Indian women in Malaya is recorded. The mean milk thiamine level of 11.3 ug/100 ml. found is considerably lower than the levels recorded for milk samples from apparently "normal " women in the United Kingdom and the U.S.A.; and slightly lower than that recorded in Australia. The results suggest that the dietary
intake of thiamine in Malaya is inadequate to maintain optimum thiamine levels in the milk of lactating women.
(3) No significant difference was found in the mean thiamine content of milk from women of the three principal racial groups in Malaya ; nor was any significant difference found to be associated with the parity or age of the women or, in the case of full lactation milks, with the time after parturition at which the sample was collected. No significant difference was found in samples collected from the same woman at different times of the day or before and after a main meal.
(4) Marked differences were found in the thiamine content of samples of milk from individual women, and the possible reasons for this are discussed.
(5) The examfnation of 48 samples of milk collected during the first month after parturition confirmed previous recorded observations that the thiamine content of such milks, initially low, gradually increases to reach "normal" levels towards the end of the first month of lactation.

Matched MeSH terms: Dairy Products*
The Potential Source of B. licheniformis Contamination During Whey Protein Concentrate 80 Manufacture

Md Zain SN, Bennett R, Flint S

J Food Sci, 2017 Mar;82(3):751-756.
PMID: 28135405 DOI: 10.1111/1750-3841.13633

The objective of this study was to determine the possible source of predominant Bacillus licheniformis contamination in a whey protein concentrate (WPC) 80 manufacturing plant. Traditionally, microbial contaminants of WPC were believed to grow on the membrane surfaces of the ultrafiltration plant as this represents the largest surface area in the plant. Changes from hot to cold ultrafiltration have reduced the growth potential for bacteria on the membrane surfaces. Our recent studies of WPCs have shown the predominant microflora B. licheniformis would not grow in the membrane plant because of the low temperature (10 °C) and must be growing elsewhere. Contamination of dairy products is mostly due to bacteria being released from biofilm in the processing plant rather from the farm itself. Three different reconstituted WPC media at 1%, 5%, and 20% were used for biofilm growth and our results showed that B. licheniformis formed the best biofilm at 1% (low solids). Further investigations were done using 3 different media; tryptic soy broth, 1% reconstituted WPC80, and 1% reconstituted WPC80 enriched with lactose and minerals to examine biofilm growth of B. licheniformis on stainless steel. Thirty-three B. licheniformis isolates varied in their ability to form biofilm on stainless steel with stronger biofilm in the presence of minerals. The source of biofilms of thermo-resistant bacteria such as B. licheniformis is believed to be before the ultrafiltration zone represented by the 1% WPC with lactose and minerals where the whey protein concentration is about 0.6%.

Matched MeSH terms: Dairy Products/microbiology*
Fulltext CONSUMERS’ KNOWLEDGE, PERCEPTION, AND ATTITUDE TOWARDS NUTRITION AND HEALTH CLAIMS ON DAIRY PRODUCTS AND PURCHASE INTENTION IN JOHOR BAHRU

YAP HUI YEE, SITI NUR'AFIFAH JAAFAR

UMT Journal of Undergraduate Research, 2019;1(2):92-102.
MyJurnal

owadays the increasing prevalence of dietary disease has raised consumers’ awareness of healthy diet and lifestyle. The rise in the use of nutrition and health claims to promote health benefits in food products help consumers make effective food choices. There is very limited research regarding nutrition and health claims in Malaysia. This research focused on consumers’ knowledge, perception (i.e. perceived quality, perceived healthiness, perceived value, perceived importance, trust and perceived familiarity), and attitude towards nutrition and health claims on dairy products as well as their purchase intention towards the products, and examinethe relationship between these variables in Johor Bahru, Johor. All responses were collected by distributing questionnaire to convenience and quota sample (n=200). Data was analysed to obtain descriptive statistics and correlation analysis. The results showed that most of the consumers (46%) had moderate knowledge of nutrition and health claims on dairy products. Consumers generally had almost positive perception and attitude towards nutrition and health claims on dairy products and positive purchase intention towards dairy products. Almost all independent variables correlated with purchase intention towards dairy products. Consumer attitude had the strongest positive relationship with purchase intention (r=0.722), followed by moderate positive correlation between consumer perception and purchase intention (r=0.671), while consumer knowledge only had low relationship with purchase intention (r=0.299). This study added new knowledge regarding public purchasing behaviour towards dairy products with nutrition and health claims. This research also provided valuable information for both food manufacturers and companies to help them make effective decisions to improve the presentation of nutrition and health claims for better expression of information as well as the goodness of food products in order to attract the attention of health-conscious consumers.

Matched MeSH terms: Dairy Products
Fulltext Sub-Acute Toxicity Study of Tiger Milk Mushroom Lignosus tigris Chon S. Tan Cultivar E Sclerotium in Sprague Dawley Rats

Kong BH, Tan NH, Fung SY, Pailoor J

Front Pharmacol, 2016;7:246.
PMID: 27555822 DOI: 10.3389/fphar.2016.00246

Lignosus also known as "Tiger Milk Mushroom," is classified in the family Polyporaceae and mainly consumed for its medicinal properties in Southeast Asia and China. The sclerotium is known as the part with medicinal value and often used by the natives to treat a variety of ailments. Lignosus tigris Chon S. Tan, one of the species of the Malaysia Tiger Milk mushroom, has recently been successfully cultivated in laboratory. Earlier studies have demonstrated the L. tigris cultivar E sclerotia exhibited beneficial biomedicinal properties. This study evaluated the potential toxicity of L. tigris E sclerotia in a 28-day sub-acute oral administration in Sprague Dawley (SD) rats. L. tigris E sclerotial powder was administered orally at three different doses of 250, 500, and 1000 mg/kg to the SD rats once daily, consecutively for 28-days. Body weight of the rats was recorded and general behavior, adverse effects, and mortality were observed daily throughout the experimental period. At the end of the experiment, blood hematology and biochemistry, relative organ weights, and histopathological analysis were performed. Results showed that there were no mortality nor signs of toxicity throughout the 28-day sub-acute toxicity study. Oral administration of the L. tigris E sclerotial powder at daily dose up to 1000 mg/kg had no significant effects in body weight, relative organ weight, blood hematological and biochemistry, gross pathology, and histopathology of the organs. L. tigris E sclerotial powder did not cause any treatment-related adverse effect in the rats at different treatment dosages up to 1000 mg/kg. As the lethal dose for the rats is above 1000 mg/kg, the no-observed-adverse-effect level (NOAEL) dose is more than 1000 mg/kg.

Matched MeSH terms: Dairy Products
Fulltext Industrially produced trans fatty acids: major potential sources in Malaysian diet

Azimah, A., Azrina, A., Norhaizan, M.E., Mohd Sokhini, A.M., Daud, A.Z.

International Food Research Journal, 2013;20(3):1157-1164.
MyJurnal

This study was conducted to determine the major potential sources of industrially produced trans fatty acids (IP-TFAs) in Malaysian diet. Levels of IP-TFAs were initially determined in samples (n = 136) collected from 8 food groups (baked products, snacks, dairy products, breakfast cereals, fast foods, semisolid fats and cooking oils, and fried foods). A survey was carried out to 105 subjects aged 19-59 years old to determine the intake of IP-TFAs using Food Frequency Questionnaire (FFQ). The IP-TFAs range from 0-5.79% of food, with the highest found in semisolid fats and cooking oils (0.1-5.79%), followed by fast foods (0.04-0.86%), baked products (0.01-0.29%), breakfast cereals (0.06-0.45%), snacks (0.03-0.26%), dairy products (0-0.26%), and fried foods (0.002-0.06%). A Spearman’s Rho rank test showed that there were significant correlations (p < 0.05) between 4 of the food groups (semisolid fats and cooking oils, fast foods, fried foods, and baked products), with the total IP-TFA intakes of subjects. In general, total IP-TFAs intakes of subjects were < 1% of subjects' diet (0.067%-0.91% of subjects’ total calorie intakes). This indicate that high fat foods (semisolid fats and cooking oils, fast foods, fried foods, and baked products) were the main contributor of IP-TFAs in Malaysian diet, despite the low proportion of trans fatty acids, following their high frequency of daily intake.

Matched MeSH terms: Dairy Products
Atititude of asians to calcium and vitamin d rich foods and supplements: a systematic review

Chin Yi Chan, Norazlina Mohamed, Soelaiman Ima-nirwana, Kok-yong Chin

Sains Malaysiana, 2018;47:1801-1810.

The prevalence of osteoporosis is increasing in Asian countries. Optimum calcium intake and vitamin D level are
important to prevent osteoporosis. The attitude of the society towards calcium food sources and supplements, as well
as sun exposure, influence their bone health practices. This review aimed to summarize the current literature on the
attitude of Asians on calcium and vitamin D rich foods and supplements to understand the barriers among the Asians
in achieving adequate calcium intake and vitamin D level. Studies showed that most Asians have a negative attitude
towards calcium-rich foods, mainly dairy products due to lactose intolerance, aversion to the taste of dairy products or
the absence of motivation. Although they took supplements to fill the gap in their diet, they were not aware of the daily
recommended intake of calcium. Besides, the majority of them have a negative attitude towards sun exposure due to the
fear of getting skin cancer or tanning. As a conclusion, most of Asians are still not aware of the importance of calcium
and vitamin D for their health and their negative attitude must be corrected to prevent the rise of osteoporosis.

Matched MeSH terms: Dairy Products
Association between aflatoxin M1 excreted in human urine samples with the consumption of milk and dairy products

Mohd Redzwan S, Rosita J, Mohd Sokhini AM, Nurul Aqilah AR

Bull Environ Contam Toxicol, 2012 Dec;89(6):1115-9.
PMID: 23052590 DOI: 10.1007/s00128-012-0853-y

This study aimed to find the association between urinary aflatoxin M(1) level and milk and dairy products consumption. Of 160 morning urine samples collected, aflatoxin M(1) was detected in 61.3 % samples (n = 98) [mean ± SD = 0.0234 ± 0.0177 ng/mL; range = 0-0.0747 ng/mL]. Of these positive samples, 67.3 % (n = 66) had levels above the limit of detection. Respondents with intake of milk and dairy products above median (67.79 g/day) had significantly high level of AFM(1) compared to those with low intake. A significant and positive association (φ = 0.286) was found between milk and dairy products consumption and urinary aflatoxin M(1) level.

Matched MeSH terms: Dairy Products/statistics & numerical data*
Fulltext The trans fatty acids content of selected foods in Malaysia

Akmar ZD, Norhaizan ME, Azimah R, Azrina A, Chan YM

Malays J Nutr, 2013 Apr;19(1):87-98.
PMID: 24800387 MyJurnal

INTRODUCTION: There is a lack of information on the trans fatty acid (TFA) content in Malaysian foods. The objective of this study is to determine the TFA content of bakery products, snacks, dairy products, fast foods, cooking oils and semisolid fats, and breakfast cereals and Malaysian fast foods. This study also estimated the quantity of each isomer in the foods assayed.
METHODS: The trans fatty acid content of each food sample was assessed in duplicate by separating the fatty acid methyl esters (FAME) in a gas chromatography system equipped with HP-88 column (USA: split ratio 10: 1) for cis/trans separation. Five major TFA isomers, palmitoelaidic acid (16: 1t9), petroselaidic acid (18:1t6), elaidic acid (18:1t9), vaccenic acid (18: 1t11) and linoelaidic acid (18:2t9, 12), were measured using gas chromatography (GC) and the data were expressed in unit values of g/100 g lipid or g/100 g food.
RESULTS: The total TFA contents in the studied foods were < 0.001 g-8.77 g/100 g lipid or < 0.001 g-5.79 g/100 g foods. This value falls within the standard and international recommendation level for TFA. The measured range of specific TFA isomers were as follows: palmitoelaidic acid (< 0.001 g-0.26 g/100 g lipid), petroselaidic acid (< 0.001 g - 3.09 g/100 g lipid), elaidic acid (< 0.001 g-0.87 g/100 g lipid), vaccenic acid (< 0.001 g-0.41 g/100 g lipid) and linoelaidic acid (< 0.001 g-6.60 g/100 g lipid).
CONCLUSION: These data indicate that most of the tested foods have low TFA contents (< 1 g/100 g lipid).

Matched MeSH terms: Dairy Products/analysis
Fulltext High body fat percentage and low consumption of dairy products were associated with vitamin D inadequacy among older women in Malaysia

Leiu KH, Chin YS, Mohd Shariff Z, Arumugam M, Chan YM

PLoS One, 2020;15(2):e0228803.
PMID: 32053636 DOI: 10.1371/journal.pone.0228803

BACKGROUND: Serum vitamin D insufficiency is a public health issue, especially among older women. Sun exposure is fundamental in the production of vitamin D, but older women have less optimal sun exposure. Therefore, factors such as body composition and diet become more essential in sustaining sufficient serum levels of vitamin D. The objective of the current study is to determine factors contributing towards serum vitamin D insufficiency among 214 older women.
METHODS: The respondents had their body weight, height, waist circumference and body fat percentage measured, as well as interviewed for their socio-demographic characteristics, sun exposure and dietary intake. Fasting blood samples were obtained from the respondents to measure their serum 25-hydroxyvitamin D [25(OH)D] concentration.
RESULTS: There were 82.7% (95% CI: 77.6%, 87.8%) of the respondents that had serum vitamin D insufficiency (< 50 nmol/L) with an average of 37.4 ± 14.3nmol/L. In stepwise multiple linear regression, high percentage of body fat (ß = -0.211, p <0.01) and low consumption of milk and dairy products (ß = 0.135, p <0.05) were the main contributors towards insufficient serum vitamin D levels, but not socio-demographic characteristics, other anthropometric indices, sun exposure and diet quality.
CONCLUSION: Older women with high body fat percentage and low dairy product consumption were more likely to have serum vitamin D insufficiency. Older women should ensure their body fat percentage is within a healthy range and consume more milk and dairy products in preventing serum vitamin D insufficiency.

Matched MeSH terms: Dairy Products*
Fulltext Milk Powder Fortified with Potassium and Phytosterols to Decrease the Risk of Cardiovascular Events among the Adult Population in Malaysia: A Cost-Effectiveness Analysis

Gandola AE, Dainelli L, Zimmermann D, Dahlui M, Detzel P

Nutrients, 2019 May 30;11(6).
PMID: 31151244 DOI: 10.3390/nu11061235

This study evaluated the cost-effectiveness of the consumption of a milk powder product fortified with potassium (+1050.28 mg/day) and phytosterols (+1200 mg/day) to lower systolic blood pressure and low-density lipoprotein cholesterol, respectively, and, therefore, the risk of myocardial infarction (MI) and stroke among the 35-75-year-old population in Malaysia. A Markov model was created against a do-nothing option, from a governmental perspective, and with a time horizon of 40 years. Different data sources, encompassing clinical studies, practice guidelines, grey literature, and statistical yearbooks, were used. Sensitivity analyses were performed to evaluate the impact of uncertainty on the base case estimates. With an incremental cost-effectiveness ratio equal to international dollars (int$) 22,518.03 per quality-adjusted life-years gained, the intervention can be classified as very cost-effective. If adopted nationwide, it would help prevent at least 13,400 MIs, 30,500 strokes, and more than 10,600 and 17,100 MI- and stroke-related deaths. The discounted cost savings generated for the health care system by those who consume the fortified milk powder would amount to int$8.1 per person, corresponding to 0.7% of the total yearly health expenditure per capita. Sensitivity analyses confirmed the robustness of the results. Together with other preventive interventions, the consumption of milk powder fortified with potassium and phytosterols represents a cost-effective strategy to attenuate the rapid increase in cardiovascular burden in Malaysia.

Matched MeSH terms: Dairy Products/analysis*
Fulltext Isolation of Pediococcus acidilactici Kp10 with ability to secrete bacteriocin-like inhibitory substance from milk products for applications in food industry

Abbasiliasi S, Tan JS, Ibrahim TA, Ramanan RN, Vakhshiteh F, Mustafa S, et al.

BMC Microbiol, 2012;12:260.
PMID: 23153191 DOI: 10.1186/1471-2180-12-260

Lactic acid bacteria (LAB) can be isolated from traditional milk products. LAB that secrete substances that inhibit pathogenic bacteria and are resistant to acid, bile, and pepsin but not vancomycin may have potential in food applications.

Matched MeSH terms: Dairy Products/microbiology*
Fulltext In vitro assessment of Pediococcus acidilactici Kp10 for its potential use in the food industry

Abbasiliasi S, Tan JS, Bashokouh F, Ibrahim TAT, Mustafa S, Vakhshiteh F, et al.

BMC Microbiol, 2017 May 23;17(1):121.
PMID: 28535747 DOI: 10.1186/s12866-017-1000-z

BACKGROUND: Selection of a microbial strain for the incorporation into food products requires in vitro and in vivo evaluations. A bacteriocin-producing lactic acid bacterium (LAB), Pediococcus acidilactici Kp10, isolated from a traditional dried curd was assessed in vitro for its beneficial properties as a potential probiotic and starter culture. The inhibitory spectra of the bacterial strain against different gram-positive and gram-negative bacteria, its cell surface hydrophobicity and resistance to phenol, its haemolytic, amylolytic and proteolytic activities, ability to produce acid and coagulate milk together with its enzymatic characteristics and adhesion property were all evaluated in vitro.
RESULTS: P. acidilactici Kp10 was moderately tolerant to phenol and adhere to mammalian epithelial cells (Vero cells and ileal mucosal epithelium). The bacterium also exhibited antimicrobial activity against several gram-positive and gram-negative food-spoilage and food-borne pathogens such as Listeria monocytgenes ATCC 15313, Salmonella enterica ATCC 13311, Shigella sonnei ATCC 9290, Klebsiella oxytoca ATCC 13182, Enterobacter cloaca ATCC 35030 and Streptococcus pyogenes ATCC 12378. The absence of haemolytic activity and proteinase (trypsin) and the presence of a strong peptidase (leucine-arylamidase) and esterase-lipase (C4 and C8) were observed in this LAB strain. P. acidilactici Kp10 also produced acid, coagulated milk and has demonstrated proteolytic and amylolactic activities.
CONCLUSION: The properties exhibited by P. acidilactici Kp10 suggested its potential application as probiotic and starter culture in the food industry.

Matched MeSH terms: Dairy Products/microbiology
Fulltext Higher body mass index and lower intake of dairy products predict poor glycaemic control among Type 2 Diabetes patients in Malaysia

Shu PS, Chan YM, Huang SL

PLoS One, 2017;12(2):e0172231.
PMID: 28234927 DOI: 10.1371/journal.pone.0172231

This cross-sectional study was designed to determine factors contributing to glyceamic control in order to provide better understanding of diabetes management among Type 2 Diabetes patients. A pre-tested structured questionnaire was used to obtain information on socio-demographic and medical history. As a proxy measure for glycaemic control, glycosylated haemoglobin (HbA1c) was obtained as secondary data from the medical reports. Perceived self-care barrier on diabetes management, diet knowledge and skills, and diet quality were assessed using pretested instruments. With a response rate of 80.3%, 155 subjects were recruited for the study. Mean HbA1c level of the subjects was 9.02 ± 2.25% with more than 70% not able to achieve acceptable level in accordance to WHO recommendation. Diet quality of the subjects was unsatisfactory especially for vegetables, fruits, fish and legumes as well as from the milk and dairy products group. Higher body mass index (BMI), poorer medication compliance, lower diet knowledge and skill scores and lower intake of milk and dairy products contributed significantly on poor glycaemic control. In conclusion, while perceived self-care barriers and diet quality failed to predict HbA1c, good knowledge and skill ability, together with appropriate BMI and adequate intake of dairy products should be emphasized to optimize glycaemic control among type 2 diabetes patients.

Matched MeSH terms: Dairy Products*
Fulltext Dairy Cattle, a Potential Reservoir of Human Campylobacteriosis: Epidemiological and Molecular Characterization of Campylobacter jejuni From Cattle Farms

An JU, Ho H, Kim J, Kim WH, Kim J, Lee S, et al.

Front Microbiol, 2018;9:3136.
PMID: 30619204 DOI: 10.3389/fmicb.2018.03136

Campylobacter jejuni is a major foodborne pathogen that is increasingly found worldwide and that is transmitted to humans through meat or dairy products. A detailed understanding of the prevalence and characteristics of C. jejuni in dairy cattle farms, which are likely to become sources of contamination, is imperative and is currently lacking. In this study, a total of 295 dairy cattle farm samples from 15 farms (24 visits) in Korea were collected. C. jejuni prevalence at the farm level was 60% (9/15) and at the animal level was 23.8% (68/266). Using the multivariable generalized estimating equation (GEE) model based on farm-environmental factors, we estimated that a high density of cattle and average environmental temperature (7 days prior to sampling) below 24°C affects the presence and survival of C. jejuni in the farm environment. Cattle isolates, together with C. jejuni from other sources (chicken and human), were genetically characterized based on analysis of 10 virulence and survival genes. A total of 19 virulence profile types were identified, with type 01 carrying eight genes (all except hcp and virB11) being the most prevalent. The prevalence of virB11 and hcp was significantly higher in isolates from cattle than in those from other sources (p < 0.05). Multilocus sequence typing (MLST) of C. jejuni isolates from three different sources mainly clustered in the CC-21 and CC-48. Within the CC-21 and CC-48 clusters, cattle isolates shared an indistinguishable pattern with human isolates according to pulsed-field gel electrophoresis (PFGE) and flaA-restriction fragment length polymorphism (RFLP) typing. This suggests that CC-21 and CC-48 C. jejuni from dairy cattle are genetically related to clinical campylobacteriosis isolates. In conclusion, the farm environment influences the presence and survival of C. jejuni, which may play an important role in cycles of cattle re-infection, and dairy cattle represent potential reservoirs of human campylobacteriosis. Thus, environmental management practices could be implemented on cattle farms to reduce the shedding of C. jejuni from cattle, subsequently reducing the potential risk of the spread of cattle-derived C. jejuni to humans through the food chain.

Matched MeSH terms: Dairy Products
Fulltext Influence of miracle fruit (Synsepalum dulcificum) extract and microencapsulated Lactococcus lactis Gh1 on the antioxidant activity and probiotic viability of functional yogurt

Fazilah, N. F., Zani, N. F. A., Wasoh, H., Ariff, A., Halim, M.

International Food Research Journal, 2020;27(4):683-693.
MyJurnal

Nowadays, functional food market is dominated by dairy-based probiotic products, mainly
yogurt. The nutritional values of yogurt can be further enhanced by the inclusion of miracle
fruit (Synsepalum dulcificum) and potential probiotic Lactococcus lactis Gh1. The present
work investigated the anti-oxidative capacity and survivability of probiotic strains of six
yogurts fortified with S. dulcificum pulp extract and encapsulated L. lactis Gh1 (in
alginate-starch coating agent via extrusion technique). The flavonoid contents (TFC) were not
significantly different between yogurts, whereas the phenolic contents (TPC) showed an
increasing trend throughout the storage. Among the yogurts, the one supplemented with both
S. dulcificum and encapsulated L. lactis Gh1 showed the highest TFC (1.18 µg QE/mL) and
TPC (15.382 μg GAE/mL). The antioxidant assay (DPPH) showed a gradual increase on the
first 7 d, but decreased afterward. In comparison, yogurts fortified with S. dulcificum demonstrated higher antioxidant activity (± 80% DPPH inhibition) than the plain yogurts (± 50%
DPPH inhibition). The viability of starter cultures (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) drastically increased during the first week (log 8 ~ 10
CFU/mL) especially for yogurts containing free cell L. lactis, but subsequently decreased ( log
6 ~ 8 CFU/mL). The viability of L. lactis Gh1 in yogurts maintained at high count (log 9.43
and 9.04 CFU/mL) throughout 21 d when it was being encapsulated. In general, the fortification of S. dulcificum extract with microencapsulated L. lactis Gh1 had greatly enhanced the
quality and potential benefits of the functional yogurts.

Matched MeSH terms: Dairy Products
Fulltext Plasma isoflavones in Malaysian men according to vegetarianism and by age

Hod R, Kouidhi W, Ali Mohd M, Husain R

Asia Pac J Clin Nutr, 2016;25(1):89-96.
PMID: 26965767 DOI: 10.6133/apjcn.2016.25.1.02

Epidemiological studies indicate lower prevalences of breast and prostate cancers and cardiovascular disease in Southeast Asia where vegetarianism is popular and diets are traditionally high in phytoestrogens. This study assessed plasma isoflavones in vegetarian and non-vegetarian Malaysian men according to age. Daidzein, genistein, equol (a daidzein metabolite), formononetin, biochanin A, estrone, estradiol and testosterone were measured by validated liquid chromatography tandem mass spectrometry (LCMSMS). Plasma isoflavone and sex hormone concentrations were measured in 225 subjects according to age (18-34, 35-44 and 45-67 years old). In all age groups, vegetarians had a higher concentration of circulating isoflavones compared with non-vegetarians especially in the 45-67 year age group where all isoflavones except equol, were significantly higher in vegetarians compared with omnivores. By contrast, the 18-34 year group had a significantly higher concentration of daidzein in vegetarians and significantly higher testosterone and estrone concentrations compared with non-vegetarians. In this age group there were weak correlations between estrone, estradiol and testosterone with some of the isoflavones. This human study provides the first Malaysian data for the phytoestrogen status of vegetarian and nonvegetarian men.

Matched MeSH terms: Dairy Products

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