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  1. Faisal, M., Moniruddin, C., Alauddin, A.B.M.C.
    JUMMEC, 2017;20(2):1-7.
    MyJurnal
    Tuberculosis (TB) is a major public health problem worldwide. It is estimated that 2 billion people, a third of
    the world population, have TB infection, but are not down with the disease. Globally, incident cases of TB
    showed a rising trend, with a 6.6 million reported in 1990, 8.3 million in 2000, 9.24 million in 2004, and an
    estimated 9.27 million incident cases in 2007. The aim of this study was to evaluate the treatment outcome of
    TB patients in Nigeria in the state of Jigawa. A cross sectional retrospective study was conducted to evaluate the
    treatment outcome in directly observed treatment with a short course for tuberculosis (TB DOTS) in facilities
    in the state between the years 2010 to 2014. The study population were all the patients with TB, who had
    access to DOTS therapy. Data were collected from the various local governmental areas for tuberculosis control
    (LGA TB) register. The LGA TB control registers contained basic information of the patients, and a statistical
    software SPSS-V22.0 was used to analyse the data. A total of 963 TB patients were studied. More than half
    (57.4%) of the patients were male, and nearly three- fourths (71.2%) of the patients accessed care from urban
    local government areas in the state. The greater majority (96.3%) of the cases had pulmonary tuberculosis
    (PTB). Among the patients, more than two-fifths (45%) were cured, and a little over one-fifth (20.6%) of them
    were HIV positive. This study revealed that the treatment success rate (TSR) in the Jigawa State of Nigeria
    was higher than the overall TSR of Nigeria, and the defaulter rate in this state was lower than the Nigerian
    average. The aim of this study was to evaluate the treatment outcome of TB patients in Nigeria in the state
    of Jigawa. A cross sectional retrospective study was conducted to evaluate the treatment outcome in directly
    observed treatment with a short course for tuberculosis (TB DOTS) in facilities in the state between the years
    2010 to 2014. The study population were all the patients with TB, who had access to DOTS therapy. Data were
    collected from the various local governmental areas for tuberculosis control (LGA TB) register. The LGA TB
    control registers contained basic information of the patients, and a statistical software SPSS-V22.0 was used
    to analyse the data. A total of 963 TB patients were studied. More than half (57.4%) of the patients were
    male, and nearly three- fourths (71.2%) of the patients accessed care from urban local government areas in
    the state. The greater majority (96.3%) of the cases had pulmonary tuberculosis (PTB). Among the patients,
    more than two-fifths (45%) were cured, and a little over one-fifth (20.6%) of them were HIV positive. This study
    revealed that the treatment success rate (TSR) in the Jigawa State of Nigeria was higher than the overall TSR
    of Nigeria, and the defaulter rate in this state was lower than the Nigerian average.
    Matched MeSH terms: Software
  2. Shazmeen Daniar Shamsuddin, Nurlyana Omar, Koh, Meng-Hock
    MATEMATIKA, 2017;33(2):149-157.
    MyJurnal
    It has come to attention that Malaysia have been aiming to build its own
    nuclear power plant (NPP) for electricity generation in 2030 to diversify the national
    energy supply and resources. As part of the regulation to build a NPP, environmental
    risk assessment analysis which includes the atmospheric dispersion assessment has to
    be performed as required by the Malaysian Atomic Energy Licensing Board (AELB)
    prior to the commissioning process. The assessment is to investigate the dispersion of
    radioactive effluent from the NPP in the event of nuclear accident. This article will focus
    on current development of locally developed atmospheric dispersion modeling code
    based on Gaussian Plume model. The code is written in Fortran computer language
    and has been benchmarked to a readily available HotSpot software. The radionuclide
    release rate entering the Gaussian equation is approximated to the value found in the
    Fukushima NPP accident in 2011. Meteorological data of Mersing District, Johor of
    year 2013 is utilized for the calculations. The results show that the dispersion of radionuclide
    effluent can potentially affect areas around Johor Bahru district, Singapore
    and some parts of Riau when the wind direction blows from the North-northeast direction.
    The results from our code was found to be in good agreement with the one
    obtained from HotSpot, with less than 1% discrepancy between the two.
    Matched MeSH terms: Software
  3. Yusof MI, Shaharudin S, Sivalingarajah P
    Asian Spine J, 2018 Apr;12(2):349-355.
    PMID: 29713418 DOI: 10.4184/asj.2018.12.2.349
    Study Design: Comparative cross-sectional study.

    Purpose: We measured the vertical ground reaction force (vGRF) of the hip, knee, and ankle joints during normal gait in normal patients, adolescent idiopathic scoliosis (AIS) patients with a Cobb angle <40° and in AIS patients with spinal fusion. We aimed to investigate whether vGRF in the aforementioned joints is altered in these three groups of patients.

    Overview of Literature: vGRF of the lower limb joints may be altered in these groups of patients. Although it is known that excessive force in the joints may induce early arthritis, there is limited relevant information in the literatures.

    Methods: We measured vGRF of the hip, knee, and ankle joints during heel strike, early stance, mid stance, and toe-off phases in normal subjects (group 1, n=14), AIS patients with Cobb angle <40° (group 2, n=14), and AIS patients with spinal fusion (group 3, n=13) using a gait analysis platform. Fifteen auto-reflective tracking markers were attached to standard anatomical landmarks in both the lower limbs. The captured motion images were used to define the orientations of the body segments and force exerted on the force plate using computer software. Statistical analysis was performed using independent t-test and analysis of variance to examine differences between the right and left sides as well as those among the different subject groups.

    Results: The measurements during the four gait phases in all the groups did not show any significant difference (p>0.05). In addition, no significant difference was found in the vGRF measurements of all the joints among the three groups (p>0.05).

    Conclusions: A Cobb angle <40° and spinal fusion did not significantly create imbalance or alter vGRF of the lower limb joints in AIS patients.

    Matched MeSH terms: Software
  4. Abd Algfoor Z, Shahrizal Sunar M, Abdullah A, Kolivand H
    Brief Funct Genomics, 2017 03 01;16(2):87-98.
    PMID: 26969656 DOI: 10.1093/bfgp/elw002
    Metabolic pathways have become increasingly available for various microorganisms. Such pathways have spurred the development of a wide array of computational tools, in particular, mathematical pathfinding approaches. This article can facilitate the understanding of computational analysis of metabolic pathways in genomics. Moreover, stoichiometric and pathfinding approaches in metabolic pathway analysis are discussed. Three major types of studies are elaborated: stoichiometric identification models, pathway-based graph analysis and pathfinding approaches in cellular metabolism. Furthermore, evaluation of the outcomes of the pathways with mathematical benchmarking metrics is provided. This review would lead to better comprehension of metabolism behaviors in living cells, in terms of computed pathfinding approaches.
    Matched MeSH terms: Software
  5. Nur Hidayah Rani, Emilia Zainal Abidin, Noor Afifah Ya'acob, Karmegam Karuppiah, Irniza Rasdi
    MyJurnal
    Pineapple plantation workers are exposed to strenuous physical activities. This study aims to determine the prevalence
    of musculoskeletal symptoms (MSS), analyse the body postural risks related to work tasks and identify relevant MSS risk factors
    among pineapple plantation workers. This was a cross-sectional study performed at a pineapple plantation in Johor in 2016. MSS,
    information on socio-demographic background and occupational history were collected via structured questionnaires. Identification
    and risk assessment of ergonomic hazard and postural risk analysis were performed for a subset of workers. Data were entered into
    statistical software and analyse according to relevant objectives. A total of 108 workers participated in this study. The prevalence
    of MSS was 87.0% and was highest for the lower back (64.8%). In terms of ergonomic hazards, Harvesting were categorised as a
    task with the highest risk. Harvesting was also the task with the highest postural risk. From the multivariate analysis, lower back
    pain is mainly contributed by a working tenure of 10 to 25 years (Odds Ratio, OR: 3.90; 95% Confidence Interval, CI 1.05-14.4) and
    more than 25 years (OR: 7.45 (95% CI 1.26 to 44.0). Workers who worked more than 7-hour daily have a higher risk for reporting
    lower back pain. Pineapple plantation workers are exposed to excessive bending, twisting and carrying of heavy loads that may be
    linked to MSS. Effective preventive strategies are required to address MSS in this population in order to minimize risk for subsequent
    musculoskeletal disorders.
    Matched MeSH terms: Software
  6. Faizalhakim, A.S., Nurhidayu, S., Norizah, K.
    MyJurnal
    Rainfall-runoff information is critical for water resource and river basin management. Runoff can be estimated by using two methods; gauged method (direct measurement) and ungauged method (indirect formula and equation). The in-situ measurement provides real-time and accurate yet required time-consuming operation and inaccessibility topography. Therefore, the runoff estimation modelling and equation was developed to overcome the limitation of in-situ measurement. SCS-CN is a simple model of ungauged method, where runoff volume (Q) resulting from rainfall (P) is formulated using equation of (Q= (P-Ia) 2 / (P-Ia + S). It was known as the best technique to be adopted for large basin study where time and manpower also accessibility are limited. SCS-CN method also is widely use in prediction software as it taken into consideration of the effects of soil, properties, land cover and antecedent moisture. Curve Number is well developed in USA for the agriculture purpose with many investigations to validate and calibrate the values of curve number. It was applied in numerous river basins in temperate and other regions e.g. US, Argentina, India, China, South Korea, Palestine and Malaysia. However, the reliability of the CN in the tropics is doubtable due to different land use characteristics, soil type, climate, geological features and rainfall pattern and variability. Based on the reviewed conceptual and applications of SCS-CN in temperate and tropics, numerous studies found the SCS-CN method is reliable and practical for runoff estimation in tropics region.
    Matched MeSH terms: Software
  7. Lim, Fei Yee, Uma Subramaniam, Renukanth Raman, Chua Sim Loo
    Malaysian Dental Journal, 2016;39(1):9-25.
    MyJurnal
    Aim: To assess the prevalence and clinical features of aggressive periodontitis in newly referred patients to the Periodontal Clinic in Seremban.

    Methodology: Sample size calculation was done using Epi Info software with minimum sample estimated at 279. 583 new patients who were referred to this clinic from year 2013 to July 2015 were examined clinically and radiographically. Patients were diagnosed using American Academy of Periodontology workshop 1999 definition.

    Results: Thirty-one (5.3%) patients were identified as having aggressive periodontitis with 28 (90%) of these patients exhibiting generalized form of aggressive periodontitis. Mean age of these patients was 34.9 years. In terms of gender and ethnicity, 21 females and 18 Malays were diagnosed with Aggressive periodontitis. First molars had the highest amount of clinical attachment loss (CAL=5.4mm). The number of missing teeth at the first visit was at median 2 (Interquartile range=4). The number of prognostically hopeless teeth at first visit was at median 1 (Interquartile range=2). Canines were preserved in most of the patients.

    Conclusion: Aggressive periodontitis was found in 5.3% of our samples. It was diagnosed at mean age of 34.9 years old.
    Matched MeSH terms: Software
  8. Kathleen, M.M., Samuel, L., Felecia, C., Ng K. H., Lesley, M.B., Kasing, A.
    MyJurnal
    (GTG)5 PCR is a type of repetitive extragenic palindromic (rep)-PCR which amplifies the (GTG)5 repetitive element that lays throughout the bacterial genome. In this study, fifty, thirty-nine and forty-nine unknown bacteria were isolated from aquaculture farms in Miri, Limbang and Lundu, respectively. (GTG)5 PCR was used to screen for clonal diversity among the isolates according to sampling sites. Banding profiles obtained from electrophoresed (GTG)5 PCR products were analyzed by RAPDistance Software to generate a dendrogram of neighbor joining tree (NJT) format. Based on the constructed dendrogram, representative isolates were selected for further identification. Conserved 16S rRNA region of the selected bacteria isolates were amplified and purified DNA products were sequenced. (GTG)5 PCR is useful in differentiation of unknown bacterial isolates and 16S rRNA analysis species identity of the bacteria in Sarawak aquaculture environment. The high diversity of bacteria in aquaculture environment may be caused by contamination from various sources.
    Matched MeSH terms: Software
  9. Othman SA, Aidil Koay NA
    Sci Rep, 2016 08 10;6:31335.
    PMID: 27507713 DOI: 10.1038/srep31335
    We analyzed the facial features of Chinese children with repaired unilateral cleft lip and palate (UCLP) and compared them with a normal control group using a three-dimensional (3D) stereophotogrammetry camera. This cross-sectional study examined 3D measurements of the facial surfaces of 20 Chinese children with repaired UCLP and 40 unaffected Chinese children aged 7 to 12 years old, which were captured using the VECTRA 3D five-pod photosystem and analyzed using Mirror software. Twenty-five variables and two ratios were compared between both groups using independent t-test. Intra- and inter-observer reliability was determined using ten randomly selected images and analyzed using intra-class correlation coefficient test (ICC). The level of significance was set at p 
    Matched MeSH terms: Software
  10. Teh LC, Prema M, Choy MP, Letchuman GR
    Med J Malaysia, 2017 02;72(1):26-31.
    PMID: 28255136 MyJurnal
    INTRODUCTION: Specialists constitute a major 'driving force' and catalyst for growth of research in their speciality. A clearer understanding is required as to what motivates their participation in research as well as the barriers they faced. This research aims to study the attitudes, barriers and facilitators faced by specialists and to identify strategies to promote and sustain research activities in their hospitals.

    METHODOLOGY: A cross-sectional survey using selfadministered questionnaires was conducted among all specialists working in government specialist hospitals in the northern states of Malaysia.

    RESULTS: Out of 733 questionnaires distributed, 467 were returned giving a response rate of 63.7%. Ninety-nine percent of the respondents believed that research benefits patients while 93.3% think research helps in their professional development. However, 34.8% think that under their present working conditions, it is unlikely they will participate in research. The major barriers identified were lack of funds for research (81%); lack access to expertise, software or statistical analysis (78.4%); interference with daily work schedule (75.1%) and inconsistent manpower in their department (74.2%). There are three barriers with statistically significant difference between hospitals with CRC compared to hospitals without CRC; lack of funds, mentors and access to expertise, software or statistical analysis. The demographic factors, attitudes and barriers contributing to involvement in research also investigated. The main facilitators for the conduct of research are potential to benefit patients and potential for professional development.

    CONCLUSION: Taking note of the findings, the Ministry of Health can implement appropriate strategies to improve specialist participation in research.

    Matched MeSH terms: Software
  11. Htay MNN, Than NN, Abas AL, Lwin H, Moe S
    PMID: 30079358 DOI: 10.4103/jehp.jehp_144_17
    CONTEXT: Family planning is crucial for everyone within the reproductive age to promote the health and welfare of every member of the family. For the medical students, it is essential to have core knowledge, understanding of family planning concept, and competency in communication skills with the patients. The final-year medical students are posted in Maternal and Child Health Clinics for 3 weeks to gain the knowledge and practical experiences on the primary healthcare in the community.

    AIMS: The aim of this study was to explore the experiences of final-year medical students on family planning services offered at community clinics in Malaysia.

    SETTINGS AND DESIGN: This was qualitative study.

    SUBJECTS AND METHODS: This qualitative study used the data of the students' reflection written in the case reports on family planning. Coding, identification of subthemes, and themes were done by two researchers independently using RQDA software.

    STATISTICAL ANALYSIS USED: Thematic analysis.

    RESULTS: Final-year medical students who had exposure to the clinical services at primary care clinic, regarding Malay word (Klinik Kesihatan) gained the learning opportunities during family planning session such as learning by observation, clerking, and counseling the patients, understanding the barriers to utilizing services and learning for their self-improvement. These learning opportunities lead to developing the positive attitudes on their learning experiences and the positive attitudes toward the concept and services of family planning.

    CONCLUSIONS: To have the better understanding of family planning services and provide the better care to the community in the future, the clinical exposure at the primary care clinics should be promoted for medical students in Malaysia.
    Matched MeSH terms: Software
  12. Che Ismail Che Noh, Fox, Anthony William
    MyJurnal
    Ebola virus disease (EVD) is an emerging and remerging zoonosis associated with high fatality rate, mainly caused by the Zaire Ebola virus (ZEBOV) and Sudan Ebola virus (SEBOV) strains. Approximately 20 epidemics of EVD have been documented mainly in Central African countries since 1976. Currently, there are no therapeutics agents and vaccines yet approved for EVD. However, several promising therapeutics and vaccines candidates are actively undergoing various phase of clinical development. This study aims to study the EVD dynamics and evaluate the potential impacts of vaccines and other preventive measures on EVD transmission control and significance of medical intervention on outcome of the disease. An initial branch chain model of EVD dynamics was built based on data obtained from previous study. Different epidemiological scenarios for EVD with impacts of intervention were simulated using Berkeley-Madonna Version 8.3.18 software. Every reduction in the exposure rate of EBV infection by 10% produces two- to five-fold improvement in protection against EVD. Transmission control is optimum when the rate of exposure to EBV infection is reduced below 1%. Optimal control of EVD transmission can be achieved through strategic implementation of successful vaccination programme, and other preventive measures as well as rapid delivery of supportive medical care.
    Matched MeSH terms: Software
  13. Zahari M, Sulaiman SA, Othman Z, Ayob Y, Karim FA, Jamal R
    Mediterr J Hematol Infect Dis, 2018;10(1):e2018056.
    PMID: 30210749 DOI: 10.4084/MJHID.2018.056
    Background: Haemophilia A (HA) and Haemophilia B (HB) are X-linked blood disorders that are caused by various mutations in the factor VIII (F8) and factor IX (F9) genes respectively. Identification of mutations is essential as some of the mutations are associated with the development of inhibitors. This study is the first comprehensive study of the F8 mutational profile in Malaysia.

    Materials and methods: We analysed 100 unrelated HA and 15 unrelated HB patients for genetic alterations in the F8 and F9 genes by using the long-range PCR, DNA sequencing, and the multiplex-ligation-dependent probe amplification assays. The prediction software was used to confirm the effects of these mutations on factor VIII and IX proteins.

    Results: 44 (53%) of the severe HA patients were positive for F8 intron 22 inversion, and three (3.6%) were positive for intron one inversion. There were 22 novel mutations in F8, including missense (8), frameshift (9), splice site (3), large deletion (1) and nonsense (1) mutations. In HB patients, four novel mutations were identified including the splice site (1), small deletion (1), large deletion (1) and missense (1) mutation.

    Discussion: The mutational spectrum of F8 in Malaysian patients is heterogeneous, with a slightly higher frequency of intron 22 inversion in these severe HA patients when compared to other Asian populations. Identification of these mutational profiles in F8 and F9 genes among Malaysian patients will provide a useful reference for the early detection and diagnosis of HA and HB in the Malaysian population.

    Matched MeSH terms: Software
  14. Alade IO, Bagudu A, Oyehan TA, Rahman MAA, Saleh TA, Olatunji SO
    Comput Methods Programs Biomed, 2018 Sep;163:135-142.
    PMID: 30119848 DOI: 10.1016/j.cmpb.2018.05.029
    BACKGROUND AND OBJECTIVES: The refractive index of hemoglobin plays important role in hematology due to its strong correlation with the pathophysiology of different diseases. Measurement of the real part of the refractive index remains a challenge due to strong absorption of the hemoglobin especially at relevant high physiological concentrations. So far, only a few studies on direct measurement of refractive index have been reported and there are no firm agreements on the reported values of refractive index of hemoglobin due to measurement artifacts. In addition, it is time consuming, laborious and expensive to perform several experiments to obtain the refractive index of hemoglobin. In this work, we proposed a very rapid and accurate computational intelligent approach using Genetic Algorithm/Support Vector Regression models to estimate the real part of the refractive index for oxygenated and deoxygenated hemoglobin samples.

    METHODS: These models utilized experimental data of wavelengths and hemoglobin concentrations in building highly accurate Genetic Algorithm/Support Vector Regression model (GA-SVR).

    RESULTS: The developed methodology showed high accuracy as indicated by the low root mean square error values of 4.65 × 10-4 and 4.62 × 10-4 for oxygenated and deoxygenated hemoglobin, respectively. In addition, the models exhibited 99.85 and 99.84% correlation coefficients (r) for the oxygenated and deoxygenated hemoglobin, thus, validating the strong agreement between the predicted and the experimental results CONCLUSIONS: Due to the accuracy and relative simplicity of the proposed models, we envisage that these models would serve as important references for future studies on optical properties of blood.

    Matched MeSH terms: Software
  15. Hamasha, Abed Al-Hadi, Almogbel, Lolowh, Alshehri, Abeer, Alssafia, Fatimah, Alghamdi, Hanan, Alajmia, Alanoud, et al.
    MyJurnal
    Upon reviewing the literature, the prevalence of many systemic conditions such as diabetes,
    hypertension, asthma and rheumatoid arthritis were reported to be high in Saudi Arabia. The relationship of these
    conditions with tooth loss among Saudi population was not investigated. Therefore, the aim of the present study
    is to explore the relationship between tooth loss and most common medical conditions among Saudi dental
    patient. The study participants were 250 patients who were randomly selected from the College of Dentistry
    database of King Saud bin Abdulaziz University for Health Sciences (KSAU-HS) in Riyadh, Saudi Arabia.
    Participants were requested to answer self-administered questionnaires related to their demographic as well as
    general health questions concerned to the presence of systemic medical conditions. Missing teeth were
    determined after examining the orthopantogram radiographs and reviewing the Romexis and SALUD databases.
    Descriptive statistics, independent t-test and linear multiple regression model were performed using SPSS
    software. The mean number of missing teeth among the study population was 5.8 teeth per person. The mean
    number of missing teeth was higher among subjects with diabetes, hypertension, rheumatoid arthritis,
    cardiovascular diseases, or osteoporosis compared to healthy individuals. A multiple linear regression analysis
    model revealed that diabetes, hypertension and rheumatoid were significant predictors of missing teeth among
    Saudi population. These results highlight the importance of the effect of medical conditions on oral health.
    Matched MeSH terms: Software
  16. Che Hussian CHA, Raja Abd Rahman RNZ, Thean Chor AL, Salleh AB, Mohamad Ali MS
    PeerJ, 2018;6:e5833.
    PMID: 30479887 DOI: 10.7717/peerj.5833
    T1 Lipase is a thermostable secretary protein of Geobacillus zalihae strain previously expressed in a prokaryotic system and purified using three-step purification: affinity 1, affinity 2, and ion exchange chromatography (IEX). This approach is time consuming and offers low purity and recovery yield. In order to enhance the purification strategy of T1 lipase, affinity 2 was removed so that after affinity 1, the cleaved Glutathione S-transferase (GST) and matured T1 lipase could be directly separated through IEX. Therefore, a rational design of GST isoelectric point (pI) was implemented by prediction using ExPASy software in order to enhance the differences of pI values between GST and matured T1 lipase. Site-directed mutagenesis at two locations flanking the downstream region of GST sequences (H215R and G213R) was successfully performed. Double point mutations changed the charge on GST from 6.10 to 6.53. The purified lipase from the new construct GST tag mutant-T1 was successfully purified using two steps of purification with 6,849 U/mg of lipase specific activity, 33% yield, and a 44-fold increase in purification. Hence, the increment of the pI values in the GST tag fusion T1 lipase resulted in a successful direct separation through IEX and lead to successful purification.
    Matched MeSH terms: Software
  17. Kusuma SAF, Parwati I, Rostinawati T, Yusuf M, Fadhlillah M, Ahyudanari RR, et al.
    Heliyon, 2019 Nov;5(11):e02741.
    PMID: 31844694 DOI: 10.1016/j.heliyon.2019.e02741
    MPT64 is a specific protein that is secreted by Mycobacterium tuberculosis complex (MTBC). The objective of this study was to obtain optimum culture conditions for MPT64 synthetic gene expression in Escherichia coli BL21 (DE3) by response surface methodology (RSM). The RSM was undertaken to optimize the culture conditions under different cultivation conditions (medium concentration, induction time and inducer concentration), designed by the factorial Box-Bhenken using Minitab 17 statistical software. From the randomized combination, 15 treatments and three center point repetitions were obtained. Furthermore, expression methods were carried out in the flask scale fermentation in accordance with the predetermined design. Then, the MPT64 protein in the cytoplasm of E. coli cell was isolated and characterized using sodium dodecyl sulfate polyacrilamide electrophoresis (SDS-PAGE) then quantified using the ImageJ program. The optimum conditions were two-fold medium concentration (tryptone 20 mg/mL, yeast extract 10 mg/mL, and sodium chloride 20 mg/mL), 5 h of induction time and 4 mM rhamnose. The average concentration of recombinant MPT64 at optimum conditions was 0.0392 mg/mL, higher than the predicted concentration of 0.0311 mg/mL. In conclusion, the relationship between the selected optimization parameters strongly influenced the level of MPT64 gene expression in E. coli BL21 (DE3).
    Matched MeSH terms: Software
  18. Nik Hisamuddin NAR, Wan Mohd Zahiruddin WN, Mohd Yazid B, Rahmah S
    Med J Malaysia, 2019 Oct;74(5):418-424.
    PMID: 31649219
    INTRODUCTION: The purpose of this study was to investigate the effect of hyperbaric oxygen therapy (HBOT) towards diabetic foot ulcer (DFU) patients in addition to the standard wound care management.

    METHODS: Fifty-eight diabetic patients with ulcers at Wagner Grade 2 and above involved in this study after presented at two study centres of tertiary teaching hospitals. The assigned patients received conventional wound care with additional HBOT given at 2.4 ATA for 90 minutes. Patients in the control group who received conventional wound care only were treated and observed for 30 days. The progress of wound healing was observed and measured at day 0, 10, 20 and 30 of study. The data collected were analysed using SPSS software (ver. 22) to study the association of HBOT towards healing of the diabetic foot ulcers.

    RESULTS: Repeated Measures ANOVA analysis with Greenhouse-Geisser correction indicated that the means of wound size over time points (Day 0, 10, 20 and 30) among patients under HBOT group were statistically significantly different [F(1,61)=30.86, p<0.001)] compared to conventional therapy group. Multiple logistic regression analysis showed that HBOT group has nearly 44 times higher odds to achieve at least 30% wound size reduction within the study period (95%CI: 7.18, 268.97, p<0.001).

    CONCLUSION: The results obtained in this study indicated that as an adjunctive therapy to conventional wound care, HBOT affected the rate of healing in diabetic foot ulcers significantly in terms of wound size reduction when compared to administering the conventional wound care alone.

    Matched MeSH terms: Software
  19. Jacob SS, Prasad K, Rao P, Kamath A, Hegde RB, Baby PM, et al.
    Front Physiol, 2019;10:1230.
    PMID: 31649550 DOI: 10.3389/fphys.2019.01230
    Eryptosis is the suicidal destruction-process of erythrocytes, much like apoptosis of nucleated cells, in the course of which the stressed red cell undergoes cell-shrinkage, vesiculation and externalization of membrane phosphatidylserine. Currently, there exist numerous methods to detect eryptosis, both morphometrically and biochemically. This study aimed to design a simple but sensitive, automated computerized approach to instantaneously detect eryptotic red cells and quantify their hallmark morphological characteristics. Red cells from 17 healthy volunteers were exposed to normal Ringer and hyperosmotic stress with sodium chloride, following which morphometric comparisons were conducted from their photomicrographs. The proposed method was found to significantly detect and differentiate normal and eryptotic red cells, based on variations in their structural markers. The receiver operating characteristic curve analysis for each of the markers showed a significant discriminatory accuracy with high sensitivity, specificity and area under the curve values. The software-based technique was then validated with RBCs in malaria. This model, quantifies eryptosis morphometrically in real-time, with minimal manual intervention, providing a new window to explore eryptosis triggered by different stressors and diseases and can find wide application in laboratories of hematology, blood banks and medical research.
    Matched MeSH terms: Software
  20. Ivan Vun JS, Cheah WL, Helmy H
    Malays Fam Physician, 2019;14(2):18-25.
    PMID: 31827731
    Introduction: Caregivers have a high risk of mental health disorders. The quality of patient care is inseparable from the mental health status of caregivers. The objective of this research was to study the mental health status among caregivers of psychiatric patients and its associated factors.

    Method: A cross-sectional study was conducted among 198 caregivers in Kuching from January till July 2014.   The respondents were recruited using systematic sampling and were required to provide information on sociodemographic and environmental factors as well as complete the Hospital Anxiety and Depression Scale (HADS) questionnaire. The data was analysed using the IBM SPSS Statistical Software Version 20.0.

    Results: The prevalence rates of anxiety and depression among caregivers were 32.8% and 27.8%, respectively.  The caregiver's age (OR=0.97, 95% CI = 0.953 - 0.996), the perception of caregiving as an economic burden (OR= 2.70, 95% CI= 1.256 - 5.803) and the dependence of the patient (OR= 2.27, 95% CI= 1.087 - 4.719) were associated with anxiety.  A caregiver who was male (OR= 2.21, 95% CI= 1.143 - 4.262), a caretaker who held the perception that a patient was dependent on them (OR=2.53, 95% CI= 1.203 - 5.337), and a caretaker who lacked stress-coping skills (OR=2.48, 95% CI= 1.030 - 5.973) were found to be significant factors in depression.

    Conclusion: A high prevalence of probable anxiety and depression among caregivers points to the need to screen caregivers. There is a vital need to train healthcare workers to be able to detect early anxiety and depression. Culturally sensitive research should be carried out for different ethnicity, and improving the support system for caregivers is necessary.

    Matched MeSH terms: Software
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