Displaying publications 541 - 560 of 640 in total

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  1. Zhang JW, Chen S, Tomova Shakur TK, Bilgin B, Chai WJ, Ramis T, et al.
    Pers Soc Psychol Bull, 2019 09;45(9):1323-1337.
    PMID: 30658553 DOI: 10.1177/0146167218820914
    Theory and research converge to suggest that authenticity predicts positive psychological adjustment. Given these benefits of authenticity, there is a surprising dearth of research on the factors that foster authenticity. Five studies help fill this gap by testing whether self-compassion promotes subjective authenticity. Study 1 found a positive association between trait self-compassion and authenticity. Study 2 demonstrated that on days when people felt more self-compassionate, they also felt more authentic. Study 3 discovered that people experimentally induced to be self-compassionate reported greater state authenticity relative to control participants. Studies 4 and 5 recruited samples from multiple cultures and used a cross-sectional and a longitudinal design, respectively, and found that self-compassion predicts greater authenticity through reduced fear of negative evaluation (Study 4) and heightened optimism (Study 5). Across studies, self-compassion's effects on authenticity could not be accounted for by self-esteem. Overall, the results suggest that self-compassion can help cultivate subjective authenticity.
  2. Wahab AHA, Saad APM, Harun MN, Syahrom A, Ramlee MH, Sulong MA, et al.
    J Mech Behav Biomed Mater, 2019 03;91:406-415.
    PMID: 30684888 DOI: 10.1016/j.jmbbm.2018.12.033
    Intact glenoid labrum is one of passive stabilizer for glenohumeral joint, which have various stiffness at different region. The aim of this study is to develop new artificial glenoid labrum from Polyvinyl Alcohol (PVA) hydrogel, which known as good biomaterial due to its biocompatibility and ability to tailor its modulus. PVA hydrogel was formed using freeze-thaw (FT) method and the stiffness of PVA was controlled by manipulating the concentration of PVA and number of FT cycles. Then, the gradual stiffness was formed using simple diffusion method by introducing the pre-freeze-and-thaw steps. The results showed 20% PVA with three FT cycles suit to highest stiffness of glenoid labrum while 10% PVA with three FT cycles suit to lowest stiffness of glenoid labrum. The functionally graded PVA hydrogel was then developed using the same method by diffusing two mixture (20% PVA and 10% PVA). Mechanical compression test showed, the highest modulus (0.41 MPa) found at the 20% PVA region and lowest modulus (0.1 MPa) found at 10% PVA region. While, at intermediate region, the compressive modulus was in between 20% and 10%, 0.2 MPa. The existence of gradual stiffness was further prove by checking crystallinity of material at each region using Differential Scanning Calorimetry (DSC) and Wide Angle X-ray Diffraction (WAXD). Microstructure of material was obtained from Scanning Electron Microscopy (SEM). This functionally graded PVA hydrogel also able to reduce about 51% of stress at glenoid implant and up to 17% for micromotion at the interfaces. Existence of artificial glenoid labrum could minimize the occurrence of glenoid component loosening.
  3. Nawi NIM, Ong Amat S, Bilad MR, Nordin NAHM, Shamsuddin N, Prayogi S, et al.
    Polymers (Basel), 2021 Mar 22;13(6).
    PMID: 33810126 DOI: 10.3390/polym13060976
    Wastewater containing oil/water emulsion has a serious ecological impact and threatens human health. The impact worsens as its volume increases. Oil/water emulsion needs to be treated before it is discharged or reused again for processing. A membrane-based process is considered attractive in effectively treating oil/water emulsion, but progress has been dampened by the membrane fouling issue. The objective of this study is to develop polyvinylidene fluoride (PVDF) membranes customized for oil/water emulsion separation by incorporating assembly of tannic acid (TA) and polyvinylpyrrolidone (PVP) in the polymer matrix. The results show that the assembly of TA/PVP complexation was achieved as observed from the change in colour during the phase inversion and as also proven from the characterization analyses. Incorporation of the TA/PVP assembly leads to enhanced surface hydrophilicity by lowering the contact angle from 82° to 47°. In situ assembly of the TA/PVP complex also leads to enhanced clean water permeability by a factor of four as a result of enhanced mean flow pore size from 0.2 to 0.9 µm. Owing to enhanced surface chemistry and structural advantages, the optimum hydrophilic PVDF/TA/PVP membrane poses permeability of 540.18 L/(m2 h bar) for oil/water emulsion filtration, three times higher than the pristine PVDF membrane used as the reference.
  4. Butt AH, Akbar B, Aslam J, Akram N, Soudagar MEM, García Márquez FP, et al.
    Sensors (Basel), 2020 Oct 21;20(20).
    PMID: 33096774 DOI: 10.3390/s20205954
    Vertical axis wind turbines (VAWT) are a source of renewable energy and are used for both industrial and domestic purposes. The study of noise characteristics of a VAWT is an important performance parameter for the turbine. This study focuses on the development of a linear microphone array and measuring acoustic signals on a cambered five-bladed 45 W VAWT in an anechoic chamber at different tip speed ratios. The sound pressure level spectrum of VAWT shows that tonal noises such as blade passing frequencies dominate at lower frequencies whereas broadband noise corresponds to all audible ranges of frequencies. This study shows that the major portion of noise from the source is dominated by aerodynamic noises generated due to vortex generation and trailing edge serrations. The research also predicts that dynamic stall is evident in the lower Tip speed ratio (TSR) region making smaller TSR values unsuitable for a quiet VAWT. This paper compares the results of linear aeroacoustic array with a 128-MEMS acoustic camera with higher resolution. The study depicts a 3 dB margin between two systems at lower TSR values. The research approves the usage of the 8 mic linear array for small radius rotary machinery considering the results comparison with a NORSONIC camera and its resolution. These observations serve as a basis for noise reduction and blade optimization techniques.
  5. Seth EA, Lee HC, Yusof HHBM, Nordin N, Cheah YK, Ho ETW, et al.
    PLoS One, 2020;15(7):e0236826.
    PMID: 32730314 DOI: 10.1371/journal.pone.0236826
    Down syndrome (DS), is the most common cause of intellectual disability, and is characterized by defective neurogenesis during perinatal development. To identify metabolic aberrations in early neurogenesis, we profiled neurospheres derived from the embryonic brain of Ts1Cje, a mouse model of Down syndrome. High-throughput phenotypic microarray revealed a significant decrease in utilisation of 17 out of 367 substrates and significantly higher utilisation of 6 substrates in the Ts1Cje neurospheres compared to controls. Specifically, Ts1Cje neurospheres were less efficient in the utilisation of glucose-6-phosphate suggesting a dysregulation in the energy-producing pathway. T Cje neurospheres were significantly smaller in diameter than the controls. Subsequent preliminary study on supplementation with 6-phosphogluconic acid, an intermediate of glucose-6-phosphate metabolism, was able to rescue the Ts1Cje neurosphere size. This study confirmed the perturbed pentose phosphate pathway, contributing to defects observed in Ts1Cje neurospheres. We show for the first time that this comprehensive energetic assay platform facilitates the metabolic characterisation of Ts1Cje cells and confirmed their distinguishable metabolic profiles compared to the controls.
  6. Ismail J, Mustafa Kamal AH, Idris MH, Amin SMN, Hamli H, Sien LS, et al.
    Biodivers Data J, 2021;9:e67449.
    PMID: 34177311 DOI: 10.3897/BDJ.9.e67449
    Seagrass habitats are considered to be some of the most biodiverse ecosystems on the planet and safeguard some ecologically and economically important fauna, amongst which are some globally threatened species, including dugong. Malaysian seagrass ecosystems are not widespread, but their existence supports some significant marine fauna. A rigorous zooplankton study was conducted from May 2016 to February 2017, in the seagrass habitat of Lawas, Sarawak, Malaysia, to examine their temporal composition and diversity, together with their ecological influences. A total of 45 zooplankton species from 13 significant groups were recorded in the seagrass habitat. The population density of zooplankton ranged between 2,482 ind/m³ and 22,670 ind/m³ over three different seasons. A single zooplankton copepod was found to be dominant (47.40%), while bivalves were the second largest (31.8%) group in terms of total abundance. It was also noticed that the average relative abundance (0.62) and important species index (62.08) of copepods were higher than for other groups that exist in the seagrass meadow, whereas copepod Parvocalanus crassirostris showed both the highest average relative abundance (0.41) and the highest important species index (41.15). The diversity (H') and richness index of the intermediate season were found to be highest due to favourable physico-chemical conditions. Within the referred seasonal cluster, the wet and dry seasons were almost similar in terms of species abundance, while the intermediate season was distinct, with high species diversity backed by ANOSIM analysis results. Copepod and bivalves formed one group with a common similarity level of 0.80. The CCA (Canonical Correspondence Analysis) model established that abiotic factors, especially turbidity, NO2, rainfall, dissolved oxygen and pH were significantly correlated with abundance of individual groups of zooplankton. Zooplankton assemblage and abundance in Lawas were found to be very rich in multiple seasons, indicating that the productivity of uninterrupted seagrass habitat might be high and the system rich in biodiversity.
  7. Subakir H, Chong YM, Chan YF, Hasan MS, Jamaluddin MFH, Pang YK, et al.
    J Med Microbiol, 2020 Jan;69(1):49-51.
    PMID: 31750812 DOI: 10.1099/jmm.0.001108
    Introduction.Burkholderia pseudomallei (melioidosis) is an important cause of community-acquired pneumonia (CAP) in the tropics. Selective medium is recommended for laboratory diagnosis with non-sterile respiratory samples, while PCR is not routinely used due to variable reported performance. The effectiveness of these diagnostic modalities varies by site.Aim. To compare selective media and real-time PCR (qPCR) with routine media in detecting B. pseudomallei in CAP respiratory samples in a low-incidence setting in Kuala Lumpur, Malaysia.Methodology. Respiratory samples were routinely cultured on blood, chocolate and MacConkey agar (RESP-ROUTINE), and compared to culture on selective Ashdown medium (RESP-SELECTIVE) and qPCR. The gold standard was routine culture of B. pseudomallei from any site (ALL-ROUTINE).Results.B. pseudomallei was detected in 8/204 (3.9 %) samples. Overall sensitivity rates differed (P=0.03) for qPCR (100%), RESP-SELECTIVE (87.5%) and RESP-ROUTINE (50%). There was a trend towards lower median days to positive culture for RESP-SELECTIVE (1 day) compared to RESP-ROUTINE (2 days, P=0.08) and ALL-ROUTINE (2 days, P=0.06). Reagent costs for each additional detection were USD59 for RESP-SELECTIVE and USD354 for PCR.Conclusions. In a low-incidence setting, selective culture of respiratory samples on Ashdown was more sensitive and allowed quicker identification than routine media, at reasonable cost. Blood cultures are critical, confirming four cases missed by routine respiratory culture. Selective medium is useful in early pneumonia (pre-sepsis) and resource-limited settings where blood cultures are infrequently done. Real-time PCR is costly, but highly sensitive and useful for high-risk patients with diabetes, cancer or immunosuppressants, or requiring ventilation or intensive care.
  8. Arahman N, Mulyati S, Fahrina A, Muchtar S, Yusuf M, Takagi R, et al.
    Molecules, 2019 Nov 13;24(22).
    PMID: 31766222 DOI: 10.3390/molecules24224099
    The removal of impurities from water or wastewater by the membrane filtration process has become more reliable due to good hydraulic performance and high permeate quality. The filterability of the membrane can be improved by having a material with a specific pore structure and good hydrophilic properties. This work aims at preparing a polyvinylidene fluoride (PVDF) membrane incorporated with phospholipid in the form of a 2-methacryloyloxyethyl phosphorylcholine, polymeric additive in the form of polyvinylpyrrolidone, and its combination with inorganic nanosilica from a renewable source derived from bagasse. The resulting membrane morphologies were analyzed by using scanning electron microscopy. Furthermore, atomic force microscopy was performed to analyze the membrane surface roughness. The chemical compositions of the resulting membranes were identified using Fourier transform infrared. A lab-scale cross-flow filtration system module was used to evaluate the membrane's hydraulic and separation performance by the filtration of humic acid (HA) solution as the model contaminant. Results showed that the additives improved the membrane surface hydrophilicity. All modified membranes also showed up to five times higher water permeability than the pristine PVDF, thanks to the improved structure. Additionally, all membrane samples showed HA rejections of 75-90%.
  9. Abd Halim NS, Wirzal MDH, Bilad MR, Md Nordin NAH, Adi Putra Z, Sambudi NS, et al.
    Polymers (Basel), 2019 Dec 17;11(12).
    PMID: 31861059 DOI: 10.3390/polym11122117
    Electrospun nanofiber membrane (NFM) has a high potential to be applied as a filter for produced water treatment due to its highly porous structure and great permeability. However, it faces fouling issues and has low mechanical properties, which reduces the performance and lifespan of the membrane. NFM has a low integrity and the fine mat easily detaches from the sheet. In this study, nylon 6,6 was selected as the polymer since it offers great hydrophilicity. In order to increase mechanical strength and separation performance of NFM, solvent vapor treatment was implemented where the vapor induces the fusion of fibers. The fabricated nylon 6,6 NFMs were treated with different exposure times of formic acid vapor. Results show that solvent vapor treatment helps to induce the fusion of overlapping fibers. The optimum exposure time for solvent vapor is 5 h to offer full retention of dispersed oil (100% of oil rejection), has 62% higher in tensile strength (1950 MPa) compared to untreated nylon 6,6 NFM (738 MPa), and has the final permeability closest to the untreated nylon 6,6 NFM (733 L/m2.h.bar). It also took more time to get fouled (220 min) compared to untreated NFM (160 min).
  10. Mat Nawi NI, Abd Halim NS, Lee LC, Wirzal MDH, Bilad MR, Nordin NAH, et al.
    Polymers (Basel), 2020 Jan 21;12(2).
    PMID: 31973178 DOI: 10.3390/polym12020252
    The competitiveness of algae as biofuel feedstock leads to the growth of membrane filtration as one of promising technologies for algae harvesting. Nanofiber membrane (NFM) was found to be efficient for microalgae harvesting via membrane filtration, but it is highly limited by its weak mechanical strength. The main objective of this study is to enhance the applicability of nylon 6,6 NFM for microalgae filtration by optimizing the operational parameters and applying solvent vapor treatment to improve its mechanical strength. The relaxation period and filtration cycle could be optimized to improve the hydraulic performance. For a cycle of 5 min., relaxation period of ≤2 min shows the highest steady-state permeability of 365 ± 14.14 L m-2 h-1 bar-1, while for 10 min cycle, 3 min. of relaxation period was found optimum that yields permeability of 402 ± 34.47 L m-2 h-1 bar-1. The treated nylon 6,6 NFM was also used to study the effect of aeration rate. It is confirmed that the aeration rate enhances the steady-state performance for both intermittent and continuous mode of aeration. Remarkably, intermittent aeration shows 7% better permeability than the full aeration for all tested condition, which is beneficial for reducing the total energy consumption.
  11. Osman A, Mat Nawi NI, Samsuri S, Bilad MR, Shamsuddin N, Khan AL, et al.
    Polymers (Basel), 2020 Feb 12;12(2).
    PMID: 32059397 DOI: 10.3390/polym12020432
    A membrane bioreactor enhances the overall biological performance of a conventional activated sludge system for wastewater treatment by producing high-quality effluent suitable for reuse. However, membrane fouling hinders the widespread application of membrane bioreactors by reducing the hydraulic performance, shortening membrane lifespan, and increasing the operational costs for membrane fouling management. This study assesses the combined effect of membrane surface corrugation and a tilted panel in enhancing the impact of air bubbling for membrane fouling control in activated sludge filtration, applicable for membrane bioreactors. The filterability performance of such a system was further tested under variable parameters: Filtration cycle, aeration rate, and intermittent aeration. Results show that a combination of surface corrugation and panel tilting enhances the impact of aeration and leads to 87% permeance increment. The results of the parametric study shows that the highest permeance was achieved under short filtration-relaxation cycle of 5 min, high aeration rate of 1.5 L/min, and short switching period of 2.5 min, to yield the permeances of 465 ± 18, 447 ± 2, and 369 ± 9 L/(m2h bar), respectively. The high permeances lead to higher operational flux that helps to lower the membrane area as well as energy consumption. Initial estimation of the fully aerated system yields the energy input of 0.152 kWh/m3, much lower than data from the full-scale references of <0.4 kWh/m3. Further energy savings and a lower system footprint can still be achieved by applying the two-sided panel with a switching system, which will be addressed in the future.
  12. Prasetiyo WH, Naidu NBM, Sari BI, Mustofa RH, Rahmawati N, Wijaya GPA, et al.
    Data Brief, 2021 Dec;39:107569.
    PMID: 34841019 DOI: 10.1016/j.dib.2021.107569
    Dataset provides wide measurement data on internet skills, internet attitudes, computer self-efficacy of the students related to the digital citizenship in Indonesia. Due to the pandemic of Covid-19, the survey was conducted online by considering the informant consent on assessing demographic information (7 items), internet skills (9 items), internet attitude (5 items), expertise and skills in using a computer (5 items), respect (6 items), educate (5 items), and protect (4 items), which was carried out from March to April. There were a total of 581 respondents selected through probability sampling based on random convenient sample from 12 public and private senior high schools which spread throughout 5 cities in Central Java, Indonesia. The survey data were analyzed using multivariate analysis and partial least structure with the analysis technique of Structural Equation Modelling (SEM). In the future, this data can help educators, researchers, and educational policy makers to determine the level of readiness of students' digital citizenship attributes and efforts to conduct further research on efforts to strengthen digital citizenship in curricular programs.
  13. Youssouf AS, Hasbullah NF, Saidin N, Habaebi MH, Parthiban R, Bin Mohamed Zin MR, et al.
    PLoS One, 2021;16(12):e0259649.
    PMID: 34972119 DOI: 10.1371/journal.pone.0259649
    This paper provides the details of a study on the effects of electron radiation on the Performance of Inters-satellite Optical Wireless Communication (IsOWC). Academia and industry focus on solutions that can improve performance and reduce the cost of IsWOC systems. Spacecraft, space stations, satellites, and astronauts are exposed to an increased level of radiation when in space, so it is essential to evaluate the risks and performance effects associated with extended radiation exposures in missions and space travel in general. This investigation focuses on LEO, especially in the near-equatorial radiation environment. Radiation experiments supported with simulations have made it possible to obtain and evaluate the electron radiation impact on optoelectronics at the device level and system level performances. The electron radiation has induced a system degradation of 70%. This result demonstrates the importance of such an investigation to predict and take necessary and suitable reliable quality service for future space missions.
  14. Mohamad EMW, Kaundan MK, Hamzah MR, Azlan AA, Ayub SH, Tham JS, et al.
    BMC Public Health, 2020 Apr 28;20(1):580.
    PMID: 32345285 DOI: 10.1186/s12889-020-08704-7
    BACKGROUND: The European Health Literacy Survey Questionnaire (HLS-EU-Q47) is becoming a widely used tool to measure health literacy (HL), including in Malaysia. There are efforts to reduce the 47-item scale to parsimonious short item scales that still reflect the assumptions and requirements of the conceptual model. This study used confirmatory factor analysis to reduce the 47-item scale to a short scale that can offer a feasible HL screening tool with sufficient psychometric properties.

    METHODS: A cross-sectional survey was conducted on the Malaysian population based on ethnic distribution to ensure that the short version instrument reflects the country's varied ethnicities. The survey was administered by well-trained interviewers working for the Ministry of Health Malaysia. A total of 866 responses were obtained. Data was analysed using multi-factorial confirmatory factor analysis (CFA) with categorical variables.

    RESULTS: The analysis resulted in a satisfactory 18-item model. There were high correlations among the 18 items. The internal consistency reliability was robust, with no floor/ceiling effects. These results represented equivalence and consistency among the responses to items, suggesting that these items were homogenous in measuring Malaysian health literacy. The strong convergent and discriminant validity of the model makes the proposed 18 items a suitable short version of the health literacy instrument for Malaysia.

    CONCLUSIONS: The researchers propose the 18-item instrument to be named HLS-M-Q18. This short version instrument may be used in measuring health literacy in Malaysia as it achieved robust reliability, structural validity and construct validity that fulfilled goodness-of-fit criteria.
  15. Zulfiani U, Junaidi A, Nareswari C, Ali BTI, Jaafar J, Widyanto AR, et al.
    RSC Adv, 2023 Mar 08;13(12):7789-7797.
    PMID: 36909764 DOI: 10.1039/d2ra07595d
    Industrial growth can have a good impact on a country's economic growth, but it can also cause environmental problems, including water pollution. About 80% of industrial wastewater is discharged into the environment without treatment, of which 17-20% is dominated by dyes, such as methylene blue (MB) and methyl orange (MO) from the textile industry. Only about 5% of a textile dye is used in the dyeing process and the rest is discarded. This problem, of course, requires special handling considering the harmful effects to health. On the other hand, the abundance of plastic waste is increasing by 14% or 85 000 tons per year. This problem must be solved due to its film-forming properties. High-density polyethylene (HDPE) is one type of plastic used as a membrane material. Therefore, in this study, HDPE plastic waste was utilized as a membrane for dye removal. In this study, HDPE plastic waste was fabricated via a thermal-induced phase-separation method using mineral oil as a solvent at various concentrations of 8%, 10%, 13%, and 15% (w/w). All the membranes were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and contact angle measurements. The results showed that the HDPE membrane at a concentration of 15% displayed the best performance compared to the others in terms of MB rejection. The negative charge (-36.9) of the HDPE membrane was more effective for cationic dye removal compared to the anionic dye. The flux and rejection of HDPE 15% for 100 ppm MB and MO removal were 2.71 and 4.93 L m-2 h-1, and 99.72% and 89.8%, respectively. The pure water flux of the membrane was 15.01 L m-2 h-1 and the tensile strength was 0.3435 MPa.
  16. Chang XX, Mubarak NM, Karri RR, Tan YH, Khalid M, Dehghani MH, et al.
    Environ Res, 2023 Feb 15;219:115089.
    PMID: 36529332 DOI: 10.1016/j.envres.2022.115089
    In the present work, the synthesis of cellulose nanowhiskers (CNW)/chitosan nanocomposite films via deep eutectic solvents (DES) changing the chemical structures were carried out. It was observed that a pure chitosan film has broadband at 3180-3400 cm-1, indicating amide and hydroxyl groups. Upon CNW incorporation, the peak gets sharper and stronger and shifts to a greater wavelength. Further, the addition of DES infuses more elements of amide into the nanocomposite films. Moreover, the mechanical properties incorporating CNW filler into a chitosan matrix show an enhancement in tensile strength (TS), Young's modulus (YM), and elongation at break. The TS and YM increase while the elongation decrease as the CNW concentration increases. The YM of biocomposite films is increased to 723 MPa at 25% CNW into chitosan films. Besides, the TS has enhanced to 11.48 MPa at 15% CNW concentration in the biocomposite films. The elongation at break has decreased to 11.7% at 25% CNW concentration. Hence, incorporating CNW into the chitosan matrix via DES can still improve the mechanical properties of the nanocomposite films. Therefore, the application of DES results in a lower YM and TS as the films are hygroscopic. In conclusion, DES can be considered the new green solvent media for synthesizing materials. It has the potential to replace ionic liquids due to its biodegradability and non-toxic properties while preserving the character of low-vapour pressure. Besides that, chitosan can be used as potential material for applications in process industries, such as the biomedical and pharmaceutical industries. Thus, DES can be used as a green solvent and aim to reduce the toxic effect of chemicals on the environment during chemical production.
  17. Mahmud AH, Salahuddin NM, Md Jani AM, Abu Bakar NF, Zainal Abidin SAS, Mohd Zain Z, et al.
    Food Chem, 2023 Jun 15;411:135493.
    PMID: 36689871 DOI: 10.1016/j.foodchem.2023.135493
    A voltammetric immunosensor was developed for detection of porcine serum albumin (PSA) to identify raw meat products adulterated with pork. A novel strategy to fabricate multiple individual nanoporous alumina (NPA) millirods (length, 5.0 mm; diameter, 1.0 mm) as the biorecognition platform is described. Each NPA millirod was covalently bioconjugated with anti-PSA capturing antibodies (α-PSAC). Following immunocapture, the PSA bound to the α-PSAC/NPA millirod bioconjugate were tagged with gold nanoparticles (AuNPs) functionalized with anti-PSA detection antibodies as the signaling probe. Subsequently, the AuNPs were voltammetrically analyzed to quantify the target PSA. The immunosensor exhibited 100 % specificity and high sensitivity to PSA with a limit of detection (LoD) of 50 (range, 0-1000) pg/mL (R2 = 0.9907). Real-world applicability was successfully validated using pork/beef adulterated mixtures with a LoD of 0.05 % (w/w). Overall, the detection performance of the proposed immunosensor was excellent and, thus, is suitable for surveillance of food safety and quality.
  18. Qamruddin RM, Safferi RS, Mohamed Ismail Z, Salleh MS, Abd Hamid MNH, Frederic Ng VER, et al.
    PLoS Negl Trop Dis, 2023 Aug;17(8):e0011569.
    PMID: 37585486 DOI: 10.1371/journal.pntd.0011569
    Not all pit viper species are present in every state of Malaysia and their distribution varies according to altitude. There is limited information on pit viper bite incidence and its geographical distribution. This was a cross-sectional study of confirmed pit viper bite cases referred to Remote Envenomation Consultancy Services (RECS) from January 2017 to December 2020. Data was collected following the approval of institutional research ethics committee. Universal sampling methods were used. Confirmed pit viper bite cases in each state, geographical location and the antivenom used were reported. A total of 523 confirmed pit viper bite injuries occurred over the 4-year study period. The majority were Malaysians, male and young adults. Most were non-occupational related (83.9%) and involved the upper limbs (46.8%). The commonest pit viper species involved was Trimeresurus purpureomaculatus (23.7%). Green pit viper antivenom (GPAV) was the most frequent antivenom used (n = 51) with the majority of patients requiring only one dose (3 vials). This study provides a better appreciation of indigenous pit viper species distribution for each state and reflects the requirement of appropriate antivenom to be stocked in each state or district hospital.
  19. Chan LC, Mat Yassim AS, Ahmad Fuaad AAH, Leow TC, Sabri S, Radin Yahaya RS, et al.
    Sci Rep, 2023 Nov 17;13(1):20178.
    PMID: 37978223 DOI: 10.1038/s41598-023-47511-z
    COVID-19 results from SARS-CoV-2, which mutates frequently, challenging current treatments. Therefore, it is critical to develop new therapeutic drugs against this disease. This study explores the interaction between SARS-CoV-2 3CLpro and RetroMAD1, a well-characterized coronavirus protein and potential drug target, using in-silico methods. The analysis through the HDOCK server showed stable complex formation with a binding energy of -12.3, the lowest among reference drugs. The RetroMAD1-3CLpro complex underwent a 100 ns molecular dynamics simulation (MDS) in an explicit solvation system, generating various trajectories, including RMSD, RMSF, hydrogen bonding, radius of gyration, and ligand binding energy. MDS results confirmed intact interactions within the RetroMAD1-3CLpro complex during simulations. In vitro experiments validated RetroMAD1's ability to inhibit 3CLpro enzyme activity and prevent SARS-CoV-2 infection in human bronchial cells. RetroMAD1 exhibited antiviral efficacy comparable to Remdesivir without cytotoxicity at effective concentrations. These results suggest RetroMAD1 as a potential drug candidate against SARS-CoV-2, warranting further in vivo and clinical studies to assess its efficiency.
  20. Farabi K, Harneti D, Darwati, Nurlelasari, Mayanti T, Maharani R, et al.
    Nat Prod Res, 2024;38(2):227-235.
    PMID: 35994369 DOI: 10.1080/14786419.2022.2114472
    Three new dammarane-type triterpenoids, namely elliptaglin A-C (1-3) were isolated from the stem bark of Aglaia elliptica (C.DC.) Blume along with three known derivatives, namely (20S)-hydroxydammar-24-en-3-on (4), cabralealactone (5), and E-25-hydroperoxydammar-23-en-20-ol-3-one (6). Subsequently, their chemical structures were determined using HR-ESI-MS, FTIR, 1D and 2D-NMR spectroscopic analysis as well as comparison with previous studies. The cytotoxicity activities of the isolated compounds against MCF-7 breast cancer and B16-F10 melanoma cell lines were then tested using PrestoBlue reagent. The analysis results showed that elliptaglin B (2) had the strongest activity against both cell lines with IC50 values of 60.98 and 51.83 µM, respectively.
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