Displaying publications 41 - 60 of 79 in total

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  1. Nadzirah, K.Z., Zainal, S., Noriham, A., Normah, I.
    MyJurnal
    Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase extraction, cation exchange chromatography and ammonium sulphate precipitation. Of the four techniques, cation exchange chromatography had shown the best bromelain purification technique with purification fold of 10.0 followed by reverse micellar system containing CTAB/ isooctane/ hexanol/ butanol, ATPE containing PEG polymer, ammonium sulphate precipitation and ATPE containing PEO-PPO-PEO with purification fold of 5.2, 4.0, 2.81 and 1.25, respectively.
    Matched MeSH terms: Ananas
  2. Nasoha NZ, Luthfi AAI, Roslan MF, Hariz HB, Bukhari NA, Manaf SFA
    Sci Rep, 2023 Nov 07;13(1):19284.
    PMID: 37935748 DOI: 10.1038/s41598-023-46061-8
    This study explores utilizing pineapple peel (PP) hydrolysate as a promising carbon source for xylitol production, covering scopes from the pre-treatment to the fermentation process. The highest xylose concentration achieved was around 20 g/L via mild acid hydrolysis (5% nitric acid, 105 °C, 20-min residence time) with a solid loading of 10%. Two sets fermentability experiments were carried out of varying pH levels in synthetic media that includes acetic acid as the main inhibitors and hydrolysate supplemented with diverse nitrogen source. The results revealed that pH 7 exhibited the highest xylitol production, yielding 0.35 g/g. Furthermore, urea was found to be a highly promising and cost-effective substitute for yeast extract, as it yielded a comparable xylitol production of 0.31 g/g with marginal difference of only 0.01 g/g compared to yeast extract further highlights the viability of urea as the preferred option for reducing xylitol production cost. The absence of a significant difference between the synthetic media and hydrolysate, with only a marginal variance of 0.35 to 0.32 g/g, implies that acetic acid is indeed the primary constraint in xylitol production using PP hydrolysate. The study sheds light on PP biomass's potential for xylitol production, aligning economic benefits with environmental sustainability and waste management.
    Matched MeSH terms: Ananas*
  3. Nor Habibah Mohd Rosli, Wan Azlina Ahmad
    Science Letters, 2018;12(1):30-43.
    MyJurnal
    Wastewater from industrial plants such as textile, electroplating and petroleum refineries contains various substances that tend to increase the chemical oxygen demand (COD) of the wastewater. Therefore, it is desired to develop a process suitable for treating the wastewater to meet the regulatory limits. This work was conducted to investigate the potential of adapted single culture of A. baumannii, A.calcoaceticus and C.cellulans in reducing COD in real textile wastewater. The study was carried out by adapting each single culture (10% inoculums) to increasing concentration (1%, 2.5 %, 5%, 7.5 % and 10%) of textile wastewater. Then it was introduced to the textile effluent without pH adjustment for five days and the COD values were measured. The textile wastewater was supplemented with pineapple waste for bacterial growth and metabolism. Results obtained showed that pineapple waste was a good nutrient supply for the growth of the bacteria and the best concentration of textile wastewater for adaptation was at 2.5%. The results also showed that A.calcoaceticus shows highest COD reduction with 67% removal whereas A. baumannii and C.cellulans with 60% and 58% removal respectively. The outcome supported that the single culture used in this study showed considerably high reduction of COD from real textile wastewater.
    Matched MeSH terms: Ananas
  4. Nor, M. Z. M., Ramchandran, L., Duke, M., Vasiljevic, T.
    MyJurnal
    About 60% of world’s commercial enzyme products are proteases, giving promising opportunity
    to derive such enzymes sustainably from waste sources. Bromelain is a crude protease occurring
    naturally in pineapple, and it possesses properties of benefit for pharmaceutical, medical and food products. The production of bromelain involves a purification stage, normally performed by small-scale conventional operations which lead to high operating cost and low product recovery, while being difficult to scale up and produce polluting by-products. Membrane-based technology offers an alternative to produce high quality purified bromelain in a more efficient and sustainable process. This review identified the current state and future needs for utilising membrane processes for sustainable bromelain production at larger scales. It was found that declining membrane flux due to fouling have been reported, but may be effectively overcome with more appropriate (and advanced) membrane types and/or processing conditions. For example, interactions between macromolecules present in the pineapple derived bromelain mixture (particularly polysaccharides) and the membrane may cause performance limiting fouling, but can be overcome by enzymatic pre-treatment. Membrane fouling can be further reduced by the employment of ceramic membrane filters operating at optimised trans-membrane pressure, cross-flow velocity, feed pH and temperature. Two-stage ultrafiltration together with diafiltration or gas sparging was suggested as a means to reduce fouling and improve enzyme purity. Despite these promising technical findings, the review identified the need for a valid economic assessment to properly guide further work towards purifying bromelain from pineapple waste for sustainable production of commercial proteases.
    Matched MeSH terms: Ananas
  5. Nuid M, Aris A, Krishnen R, Chelliapan S, Muda K
    J Environ Manage, 2023 Oct 15;344:118501.
    PMID: 37418913 DOI: 10.1016/j.jenvman.2023.118501
    This study was to develop biogranules using a sequencing batch reactor (SBR) and to evaluate the effect of pineapple wastewater (PW) as a co-substrate for treating real textile wastewater (RTW). The biogranular system cycle was 24 h (2 stages of phase), with an anaerobic phase (17.8 h) followed by an aerobic phase (5.8 h) for every stage of the phase. The concentration of pineapple wastewater was the main factor studied in influencing COD and color removal efficiency. Pineapple wastewater with different concentrations (7, 5, 4, 3, and 0% v/v) makes a total volume of 3 L and causes the OLRs to vary from 2.90 to 0.23 kg COD/m3day. The system achieved 55% of average color removal and 88% of average COD removal at 7%v/v PW concentration during treatment. With the addition of PW, the removal increased significantly. The experiment on the treatment of RTW without any added nutrients proved the importance of co-substrate in dye degradation.
    Matched MeSH terms: Ananas*
  6. Nur Fazrina Mohamad Salleh, Ezrin Hani Sukadarin
    MyJurnal
    The prevalence of Musculoskeletal Symptoms (MSS) rapidly increases and it is recognized as a significant health outcome in agricultural sector. Agricultural ergonomics risk factor is one of hazards constantly arising from all job task activities including awkward postures and heavy lifting. Job hazard analyses (JHA) were conducted to assess the exposure of ergonomics risk factors in Malaysia Pineapple Plantation. The analyses performed involved two steps. They were: 1) guidelines and manual book Malaysia Pineapple Plantation as references; and 2) conducting walkthrough observation based on checklist approaches at the plantation. The identified risk factors were prolonged exposure of standing, squatting, stooping and kneeling, highly repetitive motion on the lower limbs, deviation and twisting of wrist and lastly, heavy lifting. The analyses confirmed that the exposure to ergonomics risk factors in pineapple plantation is high. It would be desirable to reduce the risk factors by educating and training the pineapple workers to perform their task with strong consideration of occupational safety and health.
    Matched MeSH terms: Ananas
  7. Nur Hidayah Rani, Emilia Zainal Abidin, Noor Afifah Ya'acob, Karmegam Karuppiah, Irniza Rasdi
    MyJurnal
    Pineapple plantation workers are exposed to strenuous physical activities. This study aims to determine the prevalence
    of musculoskeletal symptoms (MSS), analyse the body postural risks related to work tasks and identify relevant MSS risk factors
    among pineapple plantation workers. This was a cross-sectional study performed at a pineapple plantation in Johor in 2016. MSS,
    information on socio-demographic background and occupational history were collected via structured questionnaires. Identification
    and risk assessment of ergonomic hazard and postural risk analysis were performed for a subset of workers. Data were entered into
    statistical software and analyse according to relevant objectives. A total of 108 workers participated in this study. The prevalence
    of MSS was 87.0% and was highest for the lower back (64.8%). In terms of ergonomic hazards, Harvesting were categorised as a
    task with the highest risk. Harvesting was also the task with the highest postural risk. From the multivariate analysis, lower back
    pain is mainly contributed by a working tenure of 10 to 25 years (Odds Ratio, OR: 3.90; 95% Confidence Interval, CI 1.05-14.4) and
    more than 25 years (OR: 7.45 (95% CI 1.26 to 44.0). Workers who worked more than 7-hour daily have a higher risk for reporting
    lower back pain. Pineapple plantation workers are exposed to excessive bending, twisting and carrying of heavy loads that may be
    linked to MSS. Effective preventive strategies are required to address MSS in this population in order to minimize risk for subsequent
    musculoskeletal disorders.
    Matched MeSH terms: Ananas
  8. Nur Lisa Farhana Mohamad, Fathilah Binti Ali, Azlin Suhaida Azmi, Barre, Mohamed Soleiman, Hazleen Anuar
    MyJurnal
    The concern about our dependency on non-renewable resources and overwhelming environmental issues such as pollution caused by non-degradable packaging materials has prompted researchers to come up with alternatives to solve this problem. Thermoplastic polylactic acid (PLA) has been gaining interest due to its versatility and easy processability, thus this study was carried out to find out the properties of PLA reinforced with pineapple fibers. However, surface of the natural fibers need to be treated for better properties enhancement in the polymer matrices. Considering this, fibers were treated with 10% (w/v) concentration of potassium hydroxide (KOH) and then continued for mixing with PLA at a fixed ratio of plasticizer by using internal mixer, and then the composites were prepared into sheet via hot press. Characterization for the mechanical and morphological was conducted by using tensile testing and scanning electron microscopy, respectively. After the analysis, it is found that the surface treated pineapple fiber composite showed better elongation at break compared to untreated fiber composite. The enhance properties of PLA nanocomposites has potential to be used in various packaging materials.
    Matched MeSH terms: Ananas
  9. Ong WD, Voo LY, Kumar VS
    PLoS One, 2012;7(10):e46937.
    PMID: 23091603 DOI: 10.1371/journal.pone.0046937
    BACKGROUND: Pineapple (Ananas comosus var. comosus), is an important tropical non-climacteric fruit with high commercial potential. Understanding the mechanism and processes underlying fruit ripening would enable scientists to enhance the improvement of quality traits such as, flavor, texture, appearance and fruit sweetness. Although, the pineapple is an important fruit, there is insufficient transcriptomic or genomic information that is available in public databases. Application of high throughput transcriptome sequencing to profile the pineapple fruit transcripts is therefore needed.

    METHODOLOGY/PRINCIPAL FINDINGS: To facilitate this, we have performed transcriptome sequencing of ripe yellow pineapple fruit flesh using Illumina technology. About 4.7 millions Illumina paired-end reads were generated and assembled using the Velvet de novo assembler. The assembly produced 28,728 unique transcripts with a mean length of approximately 200 bp. Sequence similarity search against non-redundant NCBI database identified a total of 16,932 unique transcripts (58.93%) with significant hits. Out of these, 15,507 unique transcripts were assigned to gene ontology terms. Functional annotation against Kyoto Encyclopedia of Genes and Genomes pathway database identified 13,598 unique transcripts (47.33%) which were mapped to 126 pathways. The assembly revealed many transcripts that were previously unknown.

    CONCLUSIONS: The unique transcripts derived from this work have rapidly increased of the number of the pineapple fruit mRNA transcripts as it is now available in public databases. This information can be further utilized in gene expression, genomics and other functional genomics studies in pineapple.

    Matched MeSH terms: Ananas/genetics*; Ananas/metabolism
  10. Ong WD, Voo CL, Kumar SV
    Mol Biol Rep, 2012 May;39(5):5889-96.
    PMID: 22207174 DOI: 10.1007/s11033-011-1400-3
    Improving the quality of the non-climacteric fruit, pineapple, is possible with information on the expression of genes that occur during the process of fruit ripening. This can be made known though the generation of partial mRNA transcript sequences known as expressed sequence tags (ESTs). ESTs are useful not only for gene discovery but also function as a resource for the identification of molecular markers, such as simple sequence repeats (SSRs). This paper reports on firstly, the construction of a normalized library of the mature green pineapple fruit and secondly, the mining of EST-SSRs markers using the newly obtained pineapple ESTs as well as publically available pineapple ESTs deposited in GenBank. Sequencing of the clones from the EST library resulted in 282 good sequences. Assembly of sequences generated 168 unique transcripts (UTs) consisting of 34 contigs and 134 singletons with an average length of ≈500 bp. Annotation of the UTs categorized the known proteins transcripts into the three ontologies as: molecular function (34.88%), biological process (38.43%), and cellular component (26.69%). Approximately 7% (416) of the pineapple ESTs contained SSRs with an abundance of trinucleotide SSRs (48.3%) being identified. This was followed by dinucleotide and tetranucleotide SSRs with frequency of 46 and 57%, respectively. From these EST-containing SSRs, 355 (85.3%) matched to known proteins while 133 contained flanking regions for primer design. Both the ESTs were sequenced and the mined EST-SSRs will be useful in the understanding of non-climacteric ripening and the screening of biomarkers linked to fruit quality traits.
    Matched MeSH terms: Ananas/genetics*
  11. Ong, Khai Lun, Tan, Bee Wai, Liew, Siew Ling
    MyJurnal
    In this study, pineapple cannery waste materials were used as substrate for the microbial production of vanillic acid and vanillin by Aspergillus niger I-1472 and Pycnoporus cinnabarinus MUCL 39533. Biotransformation of ferulic acid from pineapple waste by A. niger I-1472 to vanillic acid was optimized using Response Surface Methodology (RSM). A central composite rotatable design was used to allocate treatment combinations and factors tested for their influence on vanillic acid production were inoculum size, yeast extract concentration, diammonium tartrate concentration and initial medium pH. The amount of vanillic acid produced was used as the response for the fermentation study and was assumed to be under the influence of the four factors tested. The estimated conditions for optimal vanillic acid production were inoculum size, 3.08 ×105 CFU mL-1; yeast extract, 0.37 gL-1; diammonium tartrate, 3.88 gL-1 and initial pH, 4.3. Subsequent biotransformation of vanillic acid by P. cinnabarinus MUCL 39533 to vanillin was enhanced with the addition of resin. Under these optimal conditions, 141.00 mgL-1 of vanillin was produced from 5 g of pineapple cannery waste.
    Matched MeSH terms: Ananas
  12. Pang WC, Ramli ANM, Hamid AAA
    J Mol Model, 2020 May 16;26(6):142.
    PMID: 32417971 DOI: 10.1007/s00894-020-04398-1
    Fruit bromelain is a cysteine protease accumulated in pineapple fruits. This proteolytic enzyme has received high demand for industrial and therapeutic applications. In this study, fruit bromelain sequences QIM61759, QIM61760 and QIM61761 were retrieved from the National Center for Biotechnology Information (NCBI) Genbank Database. The tertiary structure of fruit bromelain QIM61759, QIM61760 and QIM61761 was generated by using MODELLER. The result revealed that the local stereochemical quality of the generated models was improved by using multiple templates during modelling process. Moreover, by comparing with the available papain model, structural analysis provides an insight on how pro-peptide functions as a scaffold in fruit bromelain folding and contributing to inactivation of mature protein. The structural analysis also disclosed the similarities and differences between these models. Lastly, thermal stability of fruit bromelain was studied. Molecular dynamics simulation of fruit bromelain structures at several selected temperatures demonstrated how fruit bromelain responds to elevation of temperature.
    Matched MeSH terms: Ananas/enzymology*
  13. Polunin I
    Nature, 1951;167:442.
    LABOURERS in factories in South Malaya who cut up pineapples by hand for canning invariably show an abnormality of those parts of the body which are exposed to slight pressure and pineapple juice, notably the palmar surfaces of the fingertips and the periphery of the palms. At the beginning of the canning season, the left hand, which comes more into contact with the fruit than the knife-holding hand, becomes sore and small superficial raw areas on the fingertips are often seen. Within several days, however, these heal, and the skin ceases to be sore. The labourers state that this tolerance to the pineapple juice is due to the development of an abnormality of the skin, which in the affected area becomes bluish-white and so smooth that fingerprints may be completely lost. Deep cracks are sometimes seen in the region of the skin creases. These often stay raw and bleeding for a long time, and show no clinical signs of infection, presumably because of removal of dead tissues by enzymatic action.
    Matched MeSH terms: Ananas
  14. Pui, L. P., Ng. J. M. L., Wong, C. W.
    MyJurnal
    Spray drying is used widely for converting liquid food products into powder form as the dried powder is known to have a longer shelf life at ambient temperature, convenience to use and low transportation expenditure. In this study, the Sarawak pineapple puree was spray-dried and the characterization of the resulting powder was performed. The process of enzyme liquefaction was optimized with Pectinex® Ultra SP-L and Celluclast® 1.5 L (single and combined treatment) at different concentrations (0–2.5 %) and incubation time (0-2.5 hours). The combined treatment with both enzymes (1.5% v/w Pectinex® Ultra SP-L + 0.5% v/w Celluclast® 1.5 L, 1.5 hour) was found to be the best parameter, which produced purees with the lowest viscosity of 67.98 ± 4.27 cp. Optimization of spray drying process was carried out using different inlet temperatures (150-180°C) and maltodextrin concentrations (15-30 % w/w). Results indicated that the spraydried powder produced at 160°C with 15% w/w of maltodextrin has the highest yield (31.63 %). The spray-dried powder was further characterized for the moisture content (6.00 ± 0.63%), water activity (0.36 ± 0.01 Aw), hygroscopicity (17.35 ± 0.64%), bulk density (0.46 ± 0.04 g/ cm3 ) and solubility (87.33 ± 2.08 seconds). The fruit powder of this study can be incorporated into different fruit added –value products, such as fruit juice, yogurt, jelly and other beverages.
    Matched MeSH terms: Ananas
  15. Ramli AN, Aznan TN, Illias RM
    J Sci Food Agric, 2017 Mar;97(5):1386-1395.
    PMID: 27790704 DOI: 10.1002/jsfa.8122
    Bromelain is a mixture of proteolytic enzymes found in pineapple (Ananas comosus) plants. It can be found in several parts of the pineapple plant, including the stem, fruit, leaves and peel. High demand for bromelain has resulted in gradual increases in bromelain production. These increases have led to the need for a bromelain production strategy that yields more purified bromelain at a lower cost and with fewer production steps. Previously, bromelain was purified by conventional centrifugation, ultrafiltration and lyophilisation. Recently, the development of more modern purification techniques such as gel filtration, ion exchange chromatography, affinity chromatography, aqueous two-phase extraction and reverse micelle chromatography has resulted in increased industrial bromelain production worldwide. In addition, recombinant DNA technology has emerged as an alternative strategy for producing large amounts of ultrapure bromelain. An up-to-date compilation of data regarding the commercialisation of bromelain in the clinical, pharmaceutical and industrial fields is provided in this review. © 2016 Society of Chemical Industry.
    Matched MeSH terms: Ananas/enzymology; Ananas/chemistry
  16. Ramli ANM, Manas NHA, Hamid AAA, Hamid HA, Illias RM
    Food Chem, 2018 Nov 15;266:183-191.
    PMID: 30381175 DOI: 10.1016/j.foodchem.2018.05.125
    Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. Bromelain has been identified as an active component and as a major protease of A. comosus. Bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain. The amino acid sequence and domain analysis of the fruit and stem bromelains demonstrated several differences and similarities of these cysteine protease family members. In addition, analysis of the modelled fruit (BAA21848) and stem (CAA08861) bromelains revealed the presence of unique properties of the predicted structures. Sequence analysis and structural prediction of stem and fruit bromelains of A. comosus along with the comparison of both structures provides a new insight on their distinct properties for industrial application.
    Matched MeSH terms: Ananas/enzymology*
  17. Ranjith FH, Muhialdin BJ, Yusof NL, Mohammed NK, Miskandar MH, Hussin ASM
    Plants (Basel), 2021 Feb 03;10(2).
    PMID: 33546183 DOI: 10.3390/plants10020285
    BACKGROUND: the antagonism activity of lactic acid bacteria metabolites has the potential to prevent fungal growth on mango.

    METHODS: the potential of developing natural disinfectant while using watermelon rinds (WR), pineapple (PP), orange peels (OP), palm kernel cake (PKC), and rice bran (RB), via lacto-fermentation was investigated. The obtained lactic acid bacteria (LAB) metabolites were then employed and the in vitro antifungal activity toward five spoilage fungi of mango was tested through liquid and solid systems. Besides, the effect of the produced disinfectant on the fungal growth inhibition and quality of mango was investigated.

    RESULTS: the strains Lactobacillus plantarum ATCC8014 and Lactobacillus fermentum ATCC9338 growing in the substrates PKC and PP exhibited significantly higher in vitro antifungal activity against Colletotrichum gloeosporioides and Botryodiplodia theobromae as compared to other tested LAB strains and substrates. The in-situ results demonstrated that mango samples that were treated with the disinfectant produced from PKC fermented with L. plantarum and L. fermentum had the lowest disease incidence and disease severity index after 16 days shelf life, as well as the lowest conidial concentration. Furthermore, PKC that was fermented by L. fermentum highly maintained the quality of the mango.

    CONCLUSIONS: lactic acid fermentation of PKC by L. fermentum demonstrated a high potential for use as a natural disinfectant to control C. gloeosporioides and B. theobromae on mango.

    Matched MeSH terms: Ananas
  18. Rathnavelu V, Alitheen NB, Sohila S, Kanagesan S, Ramesh R
    Biomed Rep, 2016 Sep;5(3):283-288.
    PMID: 27602208
    Pineapple has been used as part of traditional folk medicine since ancient times and it continues to be present in various herbal preparations. Bromelain is a complex mixture of protease extracted from the fruit or stem of the pineapple plant. Although the complete molecular mechanism of action of bromelain has not been completely identified, bromelain gained universal acceptability as a phytotherapeutic agent due to its history of safe use and lack of side effects. Bromelain is widely administered for its well-recognized properties, such as its anti-inflammatory, antithrombotic and fibrinolytic affects, anticancer activity and immunomodulatory effects, in addition to being a wound healing and circulatory improvement agent. The current review describes the promising clinical applications and therapeutic properties of bromelain.
    Matched MeSH terms: Ananas
  19. Redwan RM, Saidin A, Kumar SV
    BMC Plant Biol, 2015;15:196.
    PMID: 26264372 DOI: 10.1186/s12870-015-0587-1
    Pineapple (Ananas comosus var. comosus) is known as the king of fruits for its crown and is the third most important tropical fruit after banana and citrus. The plant, which is indigenous to South America, is the most important species in the Bromeliaceae family and is largely traded for fresh fruit consumption. Here, we report the complete chloroplast sequence of the MD-2 pineapple that was sequenced using the PacBio sequencing technology.
    Matched MeSH terms: Ananas/genetics*; Ananas/metabolism
  20. Robert SD, Ismail AA, Winn T, Wolever TM
    Asia Pac J Clin Nutr, 2008;17(1):35-9.
    PMID: 18364324
    The objective of the present study was to measure the glycemic index of durian, papaya, pineapple and water-melon grown in Malaysia. Ten (10) healthy volunteers (5 females, 5 males; body mass index 21.18+/-1.7 kg/m2) consumed 50 g of available carbohydrate portions of glucose (reference food) and four test foods (durian, papaya, pineapple and watermelon) in random order after an overnight fast. Glucose was tested on three separate occasions, and the test foods were each tested once. Postprandial plasma glucose was measured at intervals for two hours after intake of the test foods. Incremental areas under the curve were calculated, and the glycemic index was determined by expressing the area under the curve after the test foods as a percentage of the mean area under the curve after glucose. The results showed that the area under the curve after pineapple, 232+/-24 mmolxmin/L, was significantly greater than those after papaya, 147+/-14, watermelon, 139+/-8, and durian, 124+/-13 mmolxmin/L (p<0.05). Similarly, the glycemic index of pineapple, 82+/-4, was significantly greater than those of papaya, 58+/-6, watermelon, 55+/-3, and durian, 49+/-5 (p<0.05). The differences in area under the curve and glycemic index among papaya, watermelon and durian were not statistically significant. We conclude that pineapple has a high glycemic index, whereas papaya is intermediate and watermelon and durian are low glycemic index foods. The validity of these results depends on the accuracy of the data in the food tables upon which the portion sizes tested were based.
    Matched MeSH terms: Ananas/chemistry
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