Displaying publications 1 - 20 of 57 in total

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  1. Resistance against apoptosis by the cellular prion protein is dependent on its glycosylation status in oral HSC-2 and colon LS 174T cancer cells
    Yap YH, Say YH
    Cancer Lett, 2011 Jul 1;306(1):111-9.
    PMID: 21439722 DOI: 10.1016/j.canlet.2011.02.040
    Most studies have focused on the role of the cellular prion protein (PrP(C)) in neurodegenerative diseases, whereas the function of this ubiquitous protein outside the nervous system remains elusive. Therefore, the anti-apoptotic property of PrP(C) in oral squamous cell carcinoma (HSC-2) and colon adenocarcinoma (LS 174T) was evaluated in this study, by stable shRNA knockdown and overexpression, respectively. PrP(C) confers resistance against oxidative stress-apoptosis as indicated by MTT assay, Annexin V-FITC/PI and DCFH-DA staining, but this property is abolished upon N-glycosylation inhibition by tunicamycin. Our results indicate that the inhibition of glycosylation in cancer cells overexpressing PrP(C) could represent a potential therapeutic target.
  2. Resistance against tumour necrosis factor α apoptosis by the cellular prion protein is cell-specific for oral, colon and kidney cancer cell lines
    Yap YH, Say YH
    Cell Biol Int, 2012 Mar 1;36(3):273-7.
    PMID: 21980981 DOI: 10.1042/CBI20110088
    Since the discovery of PrPC (cellular prion protein), most studies have focused on its role in neurodegenerative diseases, whereas its function outside the nervous system remains obscure. We investigated the ability of PrPC in resisting TNFα (tumour necrosis factor α) apoptosis in three PrPC-transiently transfected cancer cell lines, renal adenocarcinoma ACHN, oral squamous cell carcinoma HSC-2 and colon adenocarcinoma LS174T. PrPC-expressing ACHN and LS174T cells had higher viabilities compared with the mock-transfected cells, while the transient overexpression of PrPC had minimal overall effect on HSC-2 cells due to its high endogenous PrPC expression. Cell cycles were also analysed, with both PrPC expressing ACHN and LS174T cells having a significantly higher proliferative index than mock-transfected cells. Flow cytometry analysis indicated a G1/S-phase cell cycle transition in both PrPC-expressing ACHN and LS174T cells. PrPC resists TNFα apoptosis due to a modest, but statistically significant, cell-specific cytoprotection compared with mock-transfected cells.
  3. Fulltext Biodiesel synthesis catalyzed by transition metal oxides: ferric-manganese doped tungstated/molybdena nanoparticle catalyst
    Alhassan FH, Rashid U, Taufiq-Yap YH
    J Oleo Sci, 2015;64(1):91-9.
    PMID: 25492234 DOI: 10.5650/jos.ess14161
    The solid acid Ferric-manganese doped tungstated/molybdena nananoparticle catalyst was prepared via impregnation reaction followed by calcination at 600°C for 3 h. The characterization was done using X-ray diffraction (XRD), Raman spectroscopy, thermal gravimetric analysis (TGA), temperature programmed desorption of NH3 (TPD-NH3), X-ray fluorescence (XRF), Transmission electron microscope (TEM) and Brunner-Emmett-Teller surface area measurement (BET). Moreover, dependence of biodiesel yield on the reaction variables such as the reaction temperature, catalyst loading, as well as molar ratio of methanol/oil and reusability were also appraised. The catalyst was reused six times without any loss in activity with maximum yield of 92.3% ±1.12 achieved in the optimized conditions of reaction temperature of 200°C; stirring speed of 600 rpm, 1:25 molar ratio of oil to alcohol, 6 % w/w catalyst loading as well as 8 h as time of the reaction. The fuel properties of WCOME's were evaluated, including the density, kinematic viscosity, pour point, cloud point and flash point whereas all properties were compared with the limits in the ASTM D6751 standard.
  4. Fulltext Synthesis of waste cooking oil based biodiesel via ferric-manganese promoted molybdenum oxide / zirconia nanoparticle solid acid catalyst: influence of ferric and manganese dopants
    Alhassan FH, Rashid U, Taufiq-Yap YH
    J Oleo Sci, 2015;64(5):505-14.
    PMID: 25843280 DOI: 10.5650/jos.ess14228
    The utilization of ferric-manganese promoted molybdenum oxide/zirconia (Fe-Mn- MoO3/ZrO2) (FMMZ) solid acid catalyst for production of biodiesel was demonstrated. FMMZ is produced through impregnation reaction followed by calcination at 600°C for 3 h. The characterization of FMMZ had been done using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermal gravimetric analysis (TGA), temperature programmed desorption of NH3 (TPD-NH3), transmission electron microscopy(TEM) and Brunner-Emmett-Teller (BET) surface area measurement. The effect of waste cooking oil methyl esters (WCOME's) yield on the reactions variables such as reaction temperature, catalyst loading, molar ratio of methanol/oil and reusability were also assessed. The catalyst was used to convert the waste cooking oil into corresponding methyl esters (95.6%±0.15) within 5 h at 200℃ reaction temperature, 600 rpm stirring speed, 1:25 molar ratio of oil to alcohol and 4% w/w catalyst loading. The reported catalyst was successfully recycled in six connective experiments without loss in activity. Moreover, the fuel properties of WCOME's were also reported using ASTM D 6751 methods.
  5. Comparison of sonochemiluminescence images using image analysis techniques and identification of acoustic pressure fields via simulation
    Tiong TJ, Chandesa T, Yap YH
    Ultrason Sonochem, 2017 May;36:78-87.
    PMID: 28069242 DOI: 10.1016/j.ultsonch.2016.11.003
    One common method to determine the existence of cavitational activity in power ultrasonics systems is by capturing images of sonoluminescence (SL) or sonochemiluminescence (SCL) in a dark environment. Conventionally, the light emitted from SL or SCL was detected based on the number of photons. Though this method is effective, it could not identify the sonochemical zones of an ultrasonic systems. SL/SCL images, on the other hand, enable identification of 'active' sonochemical zones. However, these images often provide just qualitative data as the harvesting of light intensity data from the images is tedious and require high resolution images. In this work, we propose a new image analysis technique using pseudo-colouring images to quantify the SCL zones based on the intensities of the SCL images and followed by comparison of the active SCL zones with COMSOL simulated acoustic pressure zones.
  6. Xanthones from Garcinia mangostana (Guttiferae)
    Ee GC, Daud S, Taufiq-Yap YH, Ismail NH, Rahmani M
    Nat Prod Res, 2006 Oct;20(12):1067-73.
    PMID: 17127660
    Studies on the stem of Garcinia mangostana have led to the isolation of one new xanthone mangosharin (1) (2,6-dihydroxy-8-methoxy-5-(3-methylbut-2-enyl)-xanthone) and six other prenylated xanthones, alpha-mangostin (2), beta-mangostin (3), garcinone D (4), 1,6-dihydroxy-3,7-dimethoxy-2-(3-methylbut-2-enyl)-xanthone (5), mangostanol (6) and 5,9-dihydroxy-8- methoxy-2,2-dimethyl-7-(3-methylbut-2-enyl)-2H,6H-pyrano-[3,2-b]-xanthene-6-one (7). The structures of these compounds were determined by spectroscopic methods such as 1H NMR, 13C NMR, mass spectrometry (MS) and by comparison with previous studies. All the crude extracts when screened for their larvicidal activities indicated very good toxicity against the larvae of Aedes aegypti. This article reports the isolation and identification of the above compounds as well as bioassay data for the crude extracts. These bioassay data have not been reported before.
  7. Fulltext The effect of sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB) on the Properties of ZnO synthesized by hydrothermal method
    Ramimoghadam D, Hussein MZ, Taufiq-Yap YH
    Int J Mol Sci, 2012;13(10):13275-93.
    PMID: 23202952 DOI: 10.3390/ijms131013275
    ZnO nanostructures were synthesized by hydrothermal method using different molar ratios of cetyltrimethylammonium bromide (CTAB) and Sodium dodecyl sulfate (SDS) as structure directing agents. The effect of surfactants on the morphology of the ZnO crystals was investigated by field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) techniques. The results indicate that the mixture of cationic-anionic surfactants can significantly modify the shape and size of ZnO particles. Various structures such as flakes, sheets, rods, spheres, flowers and triangular-like particles sized from micro to nano were obtained. In order to examine the possible changes in other properties of ZnO, characterizations like powder X-ray diffraction (PXRD), thermogravimetric and differential thermogravimetric analysis (TGA-DTG), FTIR, surface area and porosity and UV-visible spectroscopy analysis were also studied and discussed.
  8. Isolation and characterization of the thrombin-like enzyme from Cryptelytrops albolabris (white-lipped tree viper) venom
    Tan NH, Fung SY, Yap YH
    Comp Biochem Physiol B Biochem Mol Biol, 2012 Jan;161(1):79-85.
    PMID: 21983189 DOI: 10.1016/j.cbpb.2011.09.009
    A thrombin-like enzyme (termed albolabrase) was isolated in purified form from the venom of Cryptelytrops albolabris (white-lipped tree viper) using high performance anion ion exchange and gel filtration chromatography. The molecular mass of albolabrase was 33.7 kDa as determined by SDS-PAGE and 35.8 kDa as determined by Superose gel filtration chromatography. The N-terminal sequence was determined to be VVGGDECNINE which is homologous to many snake venom thrombin-like enzymes. Albolabrase exhibits both arginine ester hydrolase and arginine amidase activities and the enzyme is fastidious towards tripeptide chromogenic anilide substrates. The fibrinogen clotting activity was optimum at 3mg/mL bovine fibrinogen, and showed distinct species differences in the following decreasing order: bovine fibrinogen>dog fibrinogen≈human fibrinogen>goat fibrinogen. The enzyme failed to clot both rabbit and cat fibrinogens. Reversed-phase HPLC analysis on the breakdown products of fibrinogenolytic action of albolabrase indicated that the enzyme belongs to the AB class of snake venom thrombin-like enzyme. In the indirect ELISA, IgG anti-albolabrase reacted extensively with most crotalid venoms, except with Tropidolaemus wagleri and Calloselasma rhodostoma venoms. The double sandwich ELISA, however, showed that anti-albolabrase reacted strongly only with venoms from the Trimeresurus complex, and that the results support the proposed new taxonomy changes concerning the Trimeresurus complex.
  9. Fulltext Ultrathin Assembles of Porous Array for Enhanced H2 Evolution
    Islam A, Hwa Teo S, Awual MR, Taufiq-Yap YH
    Sci Rep, 2020 Feb 11;10(1):2324.
    PMID: 32047187 DOI: 10.1038/s41598-020-59325-4
    Since the complexity of photocatalyst synthesis process and high cost of noble cocatalyst leftovers a major hurdle to producing hydrogen (H2) from water, a noble metal-free Ni-Si/MgO photocatalyst was realized for the first time to generate H2 effectively under illumination with visible light. The catalyst was produced by means of simple one-pot solid reaction using self-designed metal reactor. The physiochemical properties of photocatalyst were identified by XRD, FESEM, HRTEM, EDX, UV-visible, XPS, GC and PL. The photocatalytic activities of Ni-Si/MgO photocatalyst at different nickel concentrations were evaluated without adjusting pH, applied voltage, sacrificial agent or electron donor. The ultrathin-nanosheet with hierarchically porous structure of catalyst was found to exhibit higher photocatalytic H2 production than hexagonal nanorods structured catalyst, which suggests that the randomly branched nanosheets are more active surface to increase the light-harvesting efficiency due to its short electron diffusion path. The catalyst exhibited remarkable performance reaching up to 714 µmolh-1 which is higher among the predominant semiconductor catalyst. The results demonstrated that the photocatalytic reaction irradiated under visible light illumination through the production of hydrogen and hydroxyl radicals on metals. The outcome indicates an important step forward one-pot facile approach to prepare noble ultrathin photocatalyst for hydrogen production from water.
  10. Fulltext Investigation of the Process Conditions for Hydrogen Production by Steam Reforming of Glycerol over Ni/Al₂O₃ Catalyst Using Response Surface Methodology (RSM)
    Ebshish A, Yaakob Z, Taufiq-Yap YH, Bshish A
    Materials (Basel), 2014 Mar 19;7(3):2257-2272.
    PMID: 28788567 DOI: 10.3390/ma7032257
    In this work; a response surface methodology (RSM) was implemented to investigate the process variables in a hydrogen production system. The effects of five independent variables; namely the temperature (X₁); the flow rate (X₂); the catalyst weight (X₃); the catalyst loading (X₄) and the glycerol-water molar ratio (X₅) on the H₂ yield (Y₁) and the conversion of glycerol to gaseous products (Y₂) were explored. Using multiple regression analysis; the experimental results of the H₂ yield and the glycerol conversion to gases were fit to quadratic polynomial models. The proposed mathematical models have correlated the dependent factors well within the limits that were being examined. The best values of the process variables were a temperature of approximately 600 °C; a feed flow rate of 0.05 mL/min; a catalyst weight of 0.2 g; a catalyst loading of 20% and a glycerol-water molar ratio of approximately 12; where the H₂ yield was predicted to be 57.6% and the conversion of glycerol was predicted to be 75%. To validate the proposed models; statistical analysis using a two-sample t-test was performed; and the results showed that the models could predict the responses satisfactorily within the limits of the variables that were studied.
  11. Fulltext Seeded Growth Route to Noble Calcium Carbonate Nanocrystal
    Islam A, Teo SH, Rahman MA, Taufiq-Yap YH
    PLoS One, 2015;10(12):e0144805.
    PMID: 26700479 DOI: 10.1371/journal.pone.0144805
    A solution-phase route has been considered as the most promising route to synthesize noble nanostructures. A majority of their synthesis approaches of calcium carbonate (CaCO3) are based on either using fungi or the CO2 bubbling methods. Here, we approached the preparation of nano-precipitated calcium carbonate single crystal from salmacis sphaeroides in the presence of zwitterionic or cationic biosurfactants without external source of CO2. The calcium carbonate crystals were rhombohedron structure and regularly shaped with side dimension ranging from 33-41 nm. The high degree of morphological control of CaCO3 nanocrystals suggested that surfactants are capable of strongly interacting with the CaCO3 surface and control the nucleation and growth direction of calcium carbonate nanocrystals. Finally, the mechanism of formation of nanocrystals in light of proposed routes was also discussed.
  12. Fulltext Hydrothermal synthesis of zinc oxide nanoparticles using rice as soft biotemplate
    Ramimoghadam D, Bin Hussein MZ, Taufiq-Yap YH
    Chem Cent J, 2013;7:136.
    PMID: 23919386 DOI: 10.1186/1752-153X-7-136
    BACKGROUND: Rice as a renewable, abundant bio-resource with unique characteristics can be used as a bio-template to synthesize various functional nanomaterials. Therefore, the effect of uncooked rice flour as bio-template on physico-chemical properties, especially the morphology of zinc oxide nanostructures was investigated in this study. The ZnO particles were synthesized through hydrothermal-biotemplate method using zinc acetate-sodium hydroxide and uncooked rice flour at various ratios as precursors at 120°C for 18 hours.

    RESULTS: The results indicate that rice as a bio-template can be used to modify the shape and size of zinc oxide particles. Different morphologies, namely flake-, flower-, rose-, star- and rod-like structures were obtained with particle size at micro- and nanometer range. Pore size and texture of the resulting zinc oxide particles were found to be template-dependent and the resulting specific surface area enhanced compared to the zinc oxide synthesized without rice under the same conditions. However, optical property particularly the band gap energy is generally quite similar.

    CONCLUSION: Pure zinc oxide crystals were successfully synthesized using rice flour as biotemplate at various ratios of zinc salt to rice. The size- and shape-controlled capability of rice to assemble the ZnO particles can be employed for further useful practical applications.

  13. Fulltext Synthesis and characterization of ZnO nanostructures using palm olein as biotemplate
    Ramimoghadam D, Hussein MZ, Taufiq-Yap YH
    Chem Cent J, 2013 Apr 20;7(1):71.
    PMID: 23601826 DOI: 10.1186/1752-153X-7-71
    BACKGROUND: A green approach to synthesize nanomaterials using biotemplates has been subjected to intense research due to several advantages. Palm olein as a biotemplate offers the benefits of eco-friendliness, low-cost and scale-up for large scale production. Therefore, the effect of palm olein on morphology and surface properties of ZnO nanostructures were investigated.

    RESULTS: The results indicate that palm olein as a biotemplate can be used to modify the shape and size of ZnO particles synthesized by hydrothermal method. Different morphology including flake-, flower- and three dimensional star-like structures were obtained. FTIR study indicated the reaction between carboxyl group of palm olein and zinc species had taken place. Specific surface area enhanced while no considerable change were observed in optical properties.

    CONCLUSION: Phase-pure ZnO particles were successfully synthesized using palm olein as soft biotemplating agent by hydrothermal method. The physico-chemical properties of the resulting ZnO particles can be tuned using the ratio of palm olein to Zn cation.

  14. Fulltext Antitumour-promoting and cytotoxic constituents of etlingera elatior
    Habsah M, Ali A, Lajis N, Sukari M, Yap Y, Kikuzaki H, et al.
    Malays J Med Sci, 2005 Jan;12(1):6-12.
    PMID: 22605941
    Phytochemical studies on rhizome of Etlingera elatior have resulted in the isolation of 1,7-bis(4-hydroxyphenyl)-2,4,6-heptatrienone (1), demethoxycurcumin (2), 1,7-bis(4-hydroxyphenyl)-1,4,6-heptatrien-3-one (3), 16-hydroxylabda-8(17),11,13-trien-16,15-olide (4), stigmast-4-en-3-one (5), stigmast-4-ene-3,6-dione (6), stigmast-4-en-6b-ol-3-one (7), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (8). 1 and 4 were new compounds. Compounds 5 and 7 displayed high antitumour-promoting activity. Ethyl acetate extract showed a very significant cytotoxic activity against CEM-SS and MCF-7 cell lines (4 μg/ml and 6.25 μg/ml respectively). The antitumour-promoting activity was determined by EBV-EA assay and cytotoxic activity was determined by MTT assay.
  15. Microwave-assisted biodiesel production by esterification of palm fatty acid distillate
    Lokman IM, Rashid U, Zainal Z, Yunus R, Taufiq-Yap YH
    J Oleo Sci, 2014;63(9):849-55.
    PMID: 25099911
    In the current research work, effect of microwave irradiation energy on the esterification of palm fatty acid distillate (PFAD) to produce PFAD methyl ester / biodiesel was intensively appraised. The PFAD is a by-product from refinery of crude palm oil consisting >85% of free fatty acid (FFA). The esterification reaction process with acid catalyst is needed to convert the FFA into fatty acid methyl ester or known as biodiesel. In this work, fabricated microwave-pulse width modulation (MPWM) reactor with controlled temperature was designed to be capable to increase the PFAD biodiesel production rate. The classical optimization technique was used in order to study the relationship and the optimum condition of variables involved. Consequently, by using MPWM reactor, mixture of methanol-to-PFAD molar ratio of 9:1, 1 wt.% of sulfuric acid catalyst, at 55°C reaction temperature within 15 min reaction time gave 99.5% of FFA conversion. The quality assessment and properties of the product were analyzed according to the American Society for Testing and Materials (ASTM), European (EN) standard methods and all results were in agreement with the standard requirements. It revealed that the use of fabricated MPWM with controlled temperature was significantly affecting the rate of esterification reaction and also increased the production yield of PFAD methyl ester.
  16. A new insight to the physical interpretation of activated carbon and iron doped carbon material: sorption affinity towards organic dye
    Shah I, Adnan R, Ngah WS, Mohamed N, Taufiq-Yap YH
    Bioresour Technol, 2014 May;160:52-6.
    PMID: 24630369 DOI: 10.1016/j.biortech.2014.02.047
    To enhance the potential of activated carbon (AC), iron incorporation into the AC surface was examined in the present investigations. Iron doped activated carbon (FeAC) material was synthesized and characterized by using surface area analysis, energy dispersive X-ray (EDX), temperature programmed reduction (TPR) and temperature programmed desorption (TPD). The surface area of FeAC (543 m(2)/g) was found to be lower than AC (1043 m(2)/g) as a result of the pores widening due to diffusion of iron particles into the porous AC. Iron uploading on AC surface was confirmed through EDX analysis, showing up to 13.75 wt.% iron on FeAC surface. TPR and TPD profiles revealed the presence of more active sites on FeAC surface. FeAC have shown up to 98% methylene blue (MB) removal from the aqueous media. Thermodynamic parameters indicated the spontaneous and exothermic nature of the sorption processes.
  17. Fulltext Heterogeneous base catalysts for edible palm and non-edible Jatropha-based biodiesel production
    Lee HV, Juan JC, Binti Abdullah NF, Nizah Mf R, Taufiq-Yap YH
    Chem Cent J, 2014;8:30.
    PMID: 24812574 DOI: 10.1186/1752-153X-8-30
    Transesterification catalyzed by solid base catalyst is a brilliant technology for the noble process featuring the fast reaction under mild reacting condition in biodiesel production. Heterogeneous base catalysts are generally more reactive than solid acid catalysts which require extreme operating condition for high conversion and biodiesel yield. In the present study, synthesis of biodiesel was studied by using edible (palm) or non-edible (Jatropha) feedstock catalyzed by heterogeneous base catalysts such as supported alkali metal (NaOH/Al2O3), alkaline-earth metal oxide (MgO, CaO and SrO) and mixed metal oxides catalysts (CaMgO and CaZnO).
  18. Fulltext Soulamarin, a new coumarin from stem bark of Calophyllum soulattri
    Ee GC, Mah SH, Teh SS, Rahmani M, Go R, Taufiq-Yap YH
    Molecules, 2011 Nov 23;16(11):9721-7.
    PMID: 22113580 DOI: 10.3390/molecules16119721
    The extracts of the stem bark of Calophyllum soulattri gave a new pyranocoumarin, soulamarin (1), together with five other xanthones caloxanthone B (2), caloxanthone C (3), macluraxanthone (4), trapezifolixanthone (5) and brasixanthone B (6) one common triterpene, friedelin (7), and the steroidal triterpene stigmasterol (8). The structures of these compounds were established based on spectral evidence (1D and 2D NMR).
  19. A new furanoxanthone from the stem bark of Calophyllum inophyllum
    Ee GC, Mah SH, Rahmani M, Taufiq-Yap YH, Teh SS, Lim YM
    J Asian Nat Prod Res, 2011 Oct;13(10):956-60.
    PMID: 21972812 DOI: 10.1080/10286020.2011.600248
    The stem bark extracts of Calophyllum inophyllum furnished one new furanoxanthone, inophinnin (1), in addition to inophyllin A (2), macluraxanthone (3), pyranojacareubin (4), 4-hydroxyxanthone, friedelin, stigmasterol, and betulinic acid. The structures of these compounds were determined by spectroscopic analysis of 1D and 2D NMR spectral data ((1)H, (13)C, DEPT, COSY, HMQC, and HMBC) while EI-MS gave the molecular mass. The new xanthone, inophinnin (1), exhibited some anti-inflammatory activity in nitric oxide assay.
  20. Fulltext A novel cyclodione coumarin from the stem bark of Mesua beccariana
    Ee GC, Teh SS, Mah SH, Rahmani M, Taufiq-Yap YH, Awang K
    Molecules, 2011 Aug 25;16(9):7249-55.
    PMID: 21869752 DOI: 10.3390/molecules16097249
    Our ongoing investigations on the stem bark of Mesua beccariana afforded a novel cyclodione coumarin, beccamarin, together with two known xanthones, mesuarianone, mesuasinone, two anthraquinones, 4-methoxy-1,3,5-trihydroxy-anthraquinone and 2,5-dihydroxy-1,3,4-trimethoxyanthraquinone and one coumarin, mammea A/AB. The structures were elucidated by 1D and 2D NMR and MS techniques.
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