Browse publications by year: 1999

  1. Vimala S, Norhanom AW, Yadav M
    Br. J. Cancer, 1999 Apr;80(1-2):110-6.
    PMID: 10389986
    Zingiberaceae rhizomes commonly used in the Malaysian traditional medicine were screened for anti-tumour promoter activity using the short-term assay of inhibition of 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced Epstein-Barr virus early antigen (EBV-EA) in Raji cells. The inhibition of TPA-induced EBV-EA was detected using the indirect immunofluorescence assay (IFA) and Western blot technique. The indirect IFA detected the expression/inhibition of EBV-EA-D (diffused EA antigen), whereas the Western blot technique detected the expression/inhibition of both EBV-EA-D and EA-R (restricted EA antigen). Seven rhizomes were found to possess inhibitory activity towards EBV activation, induced by TPA; they are: Curcuma domestica, C. xanthorrhiza, Kaempferia galanga, Zingiber cassumunar, Z. officinale, Z. officinale (red variety), and Z. zerumbet. A cytotoxicity assay was carried out to determine the toxicity of the Zingiberaceae rhizome extracts. The rhizome extracts that exhibited EBV activation inhibitory activity had no cytotoxicity effect in Raji cells. Therefore, the present study shows that several Zingiberaceae species used in Malaysian traditional medicine contain naturally occurring non-toxic compounds that inhibit the EBV activation, which, if further investigated, could contribute in the development of cancer prevention methods at the tumour-promoting stage.
    MeSH terms: Antigens, Viral*; Humans; Malaysia; Phytotherapy*; Medicine, Traditional*; Plant Extracts/pharmacology*; Tumor Cells, Cultured; Blotting, Western; Anticarcinogenic Agents/pharmacology*; Chemoprevention*; Fluorescent Antibody Technique, Indirect
  2. Can. Commun. Dis. Rep., 1999 Jun 15;25(12):108-12.
    PMID: 10390905
    MeSH terms: Abattoirs; Animals; Antibodies, Viral/isolation & purification; Disease Outbreaks*; Humans; Malaysia/epidemiology; Male; Middle Aged; Respirovirus Infections/epidemiology*; Respirovirus Infections/physiopathology; Respirovirus Infections/transmission; Seasons; Singapore/epidemiology; Swine; Fatal Outcome
  3. Jackson N, Menon BS, Zarina W, Zawawi N, Naing NN
    Ann Hematol, 1999 May;78(5):233-6.
    PMID: 10391104
    Acute leukemia is more common in males at almost every age, and this fact remains unexplained. A study was carried out in northeast peninsular Malaysia, where the population is predominantly Malay, to examine whether there was a difference in ABO blood group distribution between males and females with acute leukemia (AL). The ABO blood groups of 109 male and 79 female patients with AL (98 ALL, 90 AML) were compared with those of 1019 controls. In the control population, 39.7% were group O. Among males with AL, 39.4% were group O, whereas among females with AL, the proportion was 24.1% (p=0.03). The same trend to a lower proportion of group O among females was seen if the group was divided into adult/pediatric or lymphoblastic/myeloblastic groups, though these differences were not statistically significant. If these findings can be confirmed, they suggest the presence of a "sex-responsive" gene near to the ABO gene locus on chromosome 9, which relatively protects group O women against AL, at least in our population. The existence of such a gene might also partly explain why acute leukemia, and possibly other childhood cancers, are more common in males.
    MeSH terms: ABO Blood-Group System/genetics; Adolescent; Adult; Blood Donors; Child; Child, Preschool; Humans; Infant; Leukemia/genetics*; Malaysia/epidemiology; Male; Risk Factors; Sex Ratio; Sex Determination Processes; Genetic Predisposition to Disease/genetics; Genetic Predisposition to Disease/epidemiology
  4. Haji Muhammad Ismail Hussain I, Loh WF, Sofiah A
    Brain Dev, 1999 Jun;21(4):229-35.
    PMID: 10392744
    In a cross-sectional study of 24 Oriental children with systemic lupus erythematosus (SLE) with a mean age of 11.25 years, 75% were found to have clinical and neurophysiological evidence of cerebral lupus. Seizures were the most common manifestation affecting 11 (61%) of the cases, followed by psychosis in five (27.7%), encephalopathy in five (27.7%), headaches in five (27.7%), personality changes in four (22.2%), stroke in three (16.6%), movement disorders in three (16.6%) and myelitis in one child (5.5%). Four children had cerebral lupus as the presenting manifestation of SLE. Twenty-one children had an electroencephalogram (EEG) of which 11 were normal. Abnormalities detected in the rest included focal sharps, slowing of background and electrodecremental changes. There was a poor correlation of EEG with the clinical presentation. Sixteen children with cerebral lupus had a computed tomogram (CT) of which three were normal. The commonest abnormality was cerebral atrophy with or without infarcts. Only four of the cases had lupus anticoagulant but compliment was reduced in 13. Sixteen of the cases also had renal involvement. Treatment was generally with steroids with only two patients receiving cyclophosphamide for cerebral relapse. Eight children (44%) made a full recovery. Learning disability was the most frequent sequelae affecting one-third of children seen at a 1-year follow up. Four (22%) had epilepsy, two (11%) had motor deficits and one child had optic atrophy. One child died of cerebral haemorrhage during a hypertensive crisis.
    MeSH terms: Adolescent; Brain Diseases/complications*; Brain Diseases/epidemiology*; Child; Cross-Sectional Studies; Far East/epidemiology; Female; Headache/complications; Humans; Lupus Erythematosus, Systemic/complications*; Lupus Erythematosus, Systemic/epidemiology*; Male; Psychotic Disorders/complications; Seizures/complications
  5. Chansiri K, Kawazu S, Kamio T, Terada Y, Fujisaki K, Philippe H, et al.
    Vet Parasitol, 1999 Jun 15;83(2):99-105.
    PMID: 10392966
    Classification of Theileria parasites of south-east Asian countries is still ambiguous due to the lack of basic studies, especially their molecular genetic information. In this study, we included 6 known species and 14 unclassified Theileria parasite isolates: Theileria annulata, Theileria parva, Theileria taurotragi, Theileria sergenti, Theileria buffeli, Theileria types Sable, Theileria types A, B, B1, B2, C, D, E, F, G, G1, Theileria type Medan (Indonesia), Theileria type Ipoh (Malaysia) and Theileria type Thong Song (Thailand). Small subunit ribosomal RNA (srRNA) nucleotide sequence data were collected by PCR, cloning and dideoxy sequencing. The srRNA nucleotide sequences were aligned and analyzed by distance methods, maximum parsimony algorithms and maximum likelihood methods to construct phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. The data indicated that all of the tree-building methods gave very similar results. This study identified two groups of Theileria, the pathogenic and benign groups, which are strongly supported by bootstrap analysis. The analysis also indicated that three subgroups (A, B and C) were generated within the benign Theileria group whereas the classification of Theileria type D and Thong Song is questionable. However, more basic information such as life cycle differences, vectors, modes of transmission, virulent and genetic/sexual compatability is essential for clearer taxonomic definition of the benign Theileria parasites.
    MeSH terms: Animals; Asia, Southeastern; Base Sequence; Molecular Sequence Data; Phylogeny*; RNA, Ribosomal/genetics*; RNA, Ribosomal/chemistry; Sequence Homology, Nucleic Acid; Theileriasis/parasitology; DNA, Protozoan/chemistry; Polymerase Chain Reaction; Sequence Alignment; Theileria/classification; Theileria/genetics*; Sequence Analysis, DNA
  6. Mohamad K, Martin MT, Najdar H, Gaspard C, Sévenet T, Awang K, et al.
    J Nat Prod, 1999 Jun;62(6):868-72.
    PMID: 10395505
    Nine 3,4-secoapotirucallanes, argentinic acids A-I, were isolated from the bark of Aglaia argentea and transformed to their methyl esters 1-9. The structures were determined by spectral and chemical means. Compounds 1-8 showed moderate cytotoxic activity against KB cells (IC50 1.0-3.5 microg/mL).
    MeSH terms: Antineoplastic Agents, Phytogenic/isolation & purification*; Antineoplastic Agents, Phytogenic/pharmacology; Chromatography, Thin Layer; Drug Screening Assays, Antitumor; Humans; Hydrolysis; KB Cells; Malaysia; Magnetic Resonance Spectroscopy; Plant Extracts/chemistry; Plants, Medicinal/chemistry*; Triterpenes/isolation & purification*; Triterpenes/pharmacology; Tumor Cells, Cultured
  7. Ho CC, Ahmad K
    J Colloid Interface Sci, 1999 Aug 1;216(1):25-33.
    PMID: 10395758
    The effect of metal cations, both nonhydrolyzable and hydrolyzable, on the zeta potential of palm olein emulsions stabilized by the nonionic emulsifier, polyoxyethylene nonyl phenyl ether, was investigated as a function of pH and cation concentrations, respectively. The oil drops were found to be negatively charged in the presence of simple mono- and divalent cations. Charge reversal of the oil drops was observed when hydrolyzable cations (Zn2+, Cu2+, Fe3+, and Al3+) were used and the behavior is strongly dependent on the type of cation, its concentration, and the pH of the dispersion. The results are discussed in terms of current theories of electrophoresis and adsorption-precipitation at interfaces. The chemical free energies of adsorption of the cations were calculated. Copyright 1999 Academic Press.
  8. Jing W, Ismail R
    Int J Dermatol, 1999 Jun;38(6):457-63.
    PMID: 10397587
    BACKGROUND: Mucocutaneous lesions directly related to human immunodeficiency virus (HIV) infection usually present as initial manifestations of immune deficiency. The most common mucocutaneous lesions are Kaposi's sarcoma, histoplasmosis, oro-esophageal candidiasis, oral hairy leukoplakia, and, in Asia, Penicillium marneffei infection. Non-HIV-related skin lesions, such as psoriasis, seborrheic dermatitis, and nodular prurigo, may be the initial presentation among HIV infected patients attending outpatient clinics.

    METHODS: A retrospective analysis was performed on 145 HIV-positive Malaysians of Chinese descent from two centers at the University Hospital Kuala Lumpur (UHKL) and the General Hospital Kuala Lumpur (GHKL) from March 1997 to February 1998. Demographic data and clinical data were analyzed.

    RESULTS: The analysis showed that 104 out of 145 patients had mucocutaneous disorders (71.7%). In the study, there were 100 men (96.2%) and four women (3.8%). The majority of patients were in the age group 20-50 years. The patients who presented with mucocutaneous disease also had low CD4+ T-lymphocyte counts and most had acquired immunodeficiency syndrome (AIDS) defining illness. The number of cases with generalized hyperpigmentation was very high in the group (35.9%), followed by nodular prurigo (29.7%) and xerosis (27.6%). Seborrheic dermatitis was seen in 20.7% of cases, with psoriasis in 8.3%. The most common infections were oral candidiasis (35.9%), tinea corporis and onychomycosis (9.7%), and herpes infection (5.5%); however, mucocutaneous manifestations of Kaposi's sarcoma were rare.

    CONCLUSIONS: The results suggest that mucocutaneous findings are useful clinical predictors of HIV infection or signs of the presence of advanced HIV infection.

    MeSH terms: Adult; China/ethnology; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Skin Diseases/diagnosis*; Skin Diseases/ethnology; HIV Infections/diagnosis*; HIV Infections/ethnology; CD4 Lymphocyte Count
  9. Cheah TS, Sani RA, Chandrawathani P, Bahri S, Dahlan I
    Trop Anim Health Prod, 1999 Feb;31(1):25-31.
    PMID: 10399814
    An investigation into the epidemiology of Trypansoma evansi infection in crossbred dairy cattle was conducted for a period of 12 months on a dairy cattle farm in Penninsular Malaysia. The prevalence of parasitaemia was highest in lactating animals (13.4%), followed by those in the dry herd (8.8%), late pregnant animals (8.1%), early pregnant animals (4.7%), calves (0.3%) and heifers (0.2%). The prevalence of antigenaemia was highest in the lactating animals (54.7%), followed by that in dry animals (53.7%), heifers (51.1%), late pregnant animals (47.7%), early pregnant animals (46.5%) and calves (24.2%).
    MeSH terms: Animals; Antigens, Helminth/blood; Cattle; Cattle Diseases/epidemiology*; Cattle Diseases/parasitology; Disease Outbreaks/veterinary; Enzyme-Linked Immunosorbent Assay/veterinary; Female; Lactation; Malaysia/epidemiology; Pregnancy; Seasons; Trypanosoma/pathogenicity*; Trypanosomiasis/epidemiology; Trypanosomiasis/veterinary*; Seroepidemiologic Studies
  10. Pettit GR, Tan R, Melody N, Kielty JM, Pettit RK, Herald DL, et al.
    Bioorg Med Chem, 1999 May;7(5):895-9.
    PMID: 10400343
    A Montana soil actinomycete, Streptomyces anulatus, produced (1 x 10(-2)% yield) a new cancer cell growth inhibitory cyclooctadepsipeptide named montanastatin (1) accompanied by the potent anticancer antibiotic valinomycin (2) in very high (5.1%) yields. Valinomycin but not montanastatin inhibited growth of a number of pathogenic bacteria and fungi. Interpretation of high-field (500 MHz) NMR and high-resolution FAB mass spectral data allowed assignment of the structure cyclo-(D-Val-L-Lac-L-Val-D-Hiv) to montanastatin. Valinomycin (2) was also isolated from actinomycetes cultured from a tree branch and animal feces collected in Malaysia. Streptomyces exfoliatus, isolated from the tree branch, was found to contain valinomycin in 1.6% yield, while the fecal isolate, S. anulatus, gave valinomycin in 0.9% yield.
    MeSH terms: Actinomycetales/chemistry*; Animals; Antineoplastic Agents/isolation & purification*; Antineoplastic Agents/pharmacology; Antineoplastic Agents/chemistry*; Humans; Magnetic Resonance Spectroscopy; Peptides, Cyclic/isolation & purification*; Peptides, Cyclic/pharmacology; Peptides, Cyclic/chemistry*; Tumor Cells, Cultured; Valinomycin/pharmacology; Valinomycin/chemistry; Mice
  11. Chan PWK, Debruyne JA, Goh AYT
    J Trop Pediatr, 1999 Jun;45(3):184.
    PMID: 10401205 DOI: 10.1093/tropej/45.3.184
    Study site: not reported.
    MeSH terms: Adolescent; Asthma/complications*; Child; Child, Preschool; Chronic Disease; Food Hypersensitivity/complications*; Humans; Malaysia; Surveys and Questionnaires; Risk Factors; Prevalence
  12. Hwa IA, Reimann K, Lim PK, Lai LC
    Int J Mol Med, 1999 Aug;4(2):175-8.
    PMID: 10402485
    Oestrogens play an important role in the development of breast cancer. A very important source of active oestrogens in the breast is oestrone sulphate which is converted to oestrone by oestrone sulphatase. The aim of this study was to assess the effects of IGF-I and IGF-II on oestrone sulphatase activity in, as well as cell growth of, MCF-7 and MDA-MB-231 human breast cancer cell lines. Cells were grown in supplemented DMEM and treated with varying concentrations of IGFs. At the end of the treatment period, intact cell monolayers were washed and assayed for oestrone sulphatase activity and the number of cell nuclei determined on a Coulter Counter. Oestrone sulphatase activity was significantly stimulated by IGF-I and II at concentrations of 100 ng/ml and 200 ng/ml in MCF-7 cells. IGF-I had no effect on oestrone sulphatase activity in MDA-MB-231 cells over the range of concentrations tested. Significant inhibition of oestrone sulphatase was observed in MDA-MB-231 cells at higher concentrations of IGF-II (50 ng/ml, 100 ng/ml and 200 ng/ml). Both IGF-I and IGF-II at higher concentrations (100 ng/ml and 200 ng/ml) significantly inhibited MCF-7 and stimulated MDA-MB-231 cell growth. Since IGF-I and II have effects on cell growth and oestrone sulphatase activity in breast cancer cell lines they may play a role in the development and progression of human breast cancer.
    MeSH terms: Breast Neoplasms/enzymology*; Breast Neoplasms/pathology; Cell Division/drug effects; Dose-Response Relationship, Drug; Humans; Insulin-Like Growth Factor I/pharmacology*; Insulin-Like Growth Factor II/pharmacology*; Sulfatases/drug effects*; Sulfatases/metabolism; Tumor Cells, Cultured/cytology; Tumor Cells, Cultured/drug effects; Tumor Cells, Cultured/enzymology
  13. Rahmah R, Hayati AR, Kuhnle U
    Singapore Med J, 1999 Mar;40(3):151-6.
    PMID: 10402893
    Persistent hyperinsulinaemic hypoglycaemia is a rare metabolic disorder of glucose regulation. It is however the most common cause of persistent hypoglycaemia in the neonatal period. Various drugs have been used with generally poor results, but diazoxide and a long-acting somatostatin analogue, octreotide, have been found to be rather successful. When medical therapy fails, early pancreatectomy is recommended to maintain euglycaemia. Since pancreatectomy seems to carry the long-term risk of diabetes mellitus, some authors recommend long-term medical therapy as an alternative to surgery. The outcome of treatment seems to correlate with neurological status prior to surgery. Even in early recognised and treated patients, publications suggest that a subtle neurological deficit may be present despite apparently normal intelligence. In view of the varying recommendations on treatment and the variations in outcome, we reviewed our experience over a period of three years (1992-1995) to determine whether we could formulate a rational approach to the management.
    MeSH terms: Developmental Disabilities/etiology; Developmental Disabilities/epidemiology; Female; Humans; Infant, Newborn; Male; Pancreatectomy; Pancreatic Diseases/complications; Pancreatic Diseases/epidemiology; Pancreatic Diseases/therapy*; Retrospective Studies; Treatment Outcome
  14. Khan TF, Muniandy S, Hayat FZ, Sherazi ZA, Nawaz MH
    Singapore Med J, 1999 Mar;40(3):171-3.
    PMID: 10402897
    We report three cases of Mirizzi syndrome, two with external compression of the common hepatic duct and another with cystobiliary fistula. All patients presented with jaundice. The diagnosis was suggested by ultrasonography and confirmed by endoscopic retrograde cholangiography (ERC). All three had the stones removed surgically, one through a choledochotomy, another through an opening in the gall bladder and the third at the time of subtotal cholecystectomy. We would like to propose a simple classification of Mirizzi syndrome, based on surgical procedures necessary for the correction of the pathological anatomy. If it involves the removal of calculi with some form of cholecystectomy, we consider it as Type I, whereas Type II involves the construction of a hepaticojejunostomy apart from the removal of calculi.
    MeSH terms: Cholestasis, Extrahepatic/classification*; Cholestasis, Extrahepatic/diagnosis; Cholestasis, Extrahepatic/surgery; Cholangiopancreatography, Endoscopic Retrograde; Cholelithiasis/complications*; Female; Hepatic Duct, Common*; Humans; Male; Middle Aged; Syndrome
  15. Lim KH, Chong KL
    Singapore Med J, 1999 Mar;40(3):176-8.
    PMID: 10402899
    The diagnosis of disseminated tuberculosis should be entertained in all patients with unexplained fever associated with hepatomegaly and/or splenomegaly with or without anomalies in liver function tests and haemogram. It should be considered as a possible cause of septic shock especially in patients with typical risk factors such as advanced age, diabetes, alcoholism or immunosuppression. Prompt therapy could be life saving in an otherwise potentially fatal condition. It is therefore appropriate to initiate anti-tuberculosis treatment as soon as such a diagnosis is suspected and not await final confirmation.
    MeSH terms: Adult; Diagnosis, Differential; Female; Humans; Multiple Organ Failure/etiology*; Multiple Organ Failure/physiopathology; Risk Factors; Shock, Septic/etiology*; Shock, Septic/physiopathology; Tuberculosis, Miliary/complications; Tuberculosis, Miliary/diagnosis*; Tuberculosis, Miliary/physiopathology; Fatal Outcome
  16. Kobayashi N, Thayan R, Sugimoto C, Oda K, Saat Z, Vijayamalar B, et al.
    Am J Trop Med Hyg, 1999 Jun;60(6):904-9.
    PMID: 10403318
    To characterize the dengue epidemic that recently occurred in Malaysia, we sequenced cDNAs from nine 1993-1994 dengue virus type-3 (DEN-3) isolates in Malaysia (DEN-3 was the most common type in Malaysia during this period). Nucleic acid sequences (720 nucleotides in length) from the nine isolates, encompassing the precursor of membrane protein (preM) and membrane (M) protein genes and part of the envelope (E) protein gene were aligned with various reference DEN-3 sequences to generate a neighbor-joining phylogenetic tree. According to the constructed tree, the nine Malaysian isolates were grouped into subtype II, which comprises Thai isolates from 1962 to 1987. Five earlier DEN-3 virus Malaysian isolates from 1974 to 1981 belonged to subtype I. The present data indicate that the recent dengue epidemic in Malaysia was due to the introduction of DEN-3 viruses previously endemic to Thailand.
    MeSH terms: Amino Acid Sequence; Base Sequence; Cells, Cultured; Dengue Virus/classification*; Dengue Virus/genetics; Dengue Virus/chemistry; Disease Outbreaks*; Humans; Immunoenzyme Techniques; Malaysia/epidemiology; Molecular Sequence Data; Phylogeny*; RNA, Viral/blood; RNA, Viral/chemistry; Sequence Homology, Nucleic Acid; Thailand; Viral Proteins/genetics; Viral Proteins/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Analysis, DNA; DNA Primers/chemistry; Severe Dengue/epidemiology; Severe Dengue/virology*; Reverse Transcriptase Polymerase Chain Reaction
  17. Winstanley C, Hales BA, Morgan JAW, Gallagher MJ, Puthucheary SD, CISSé MF, et al.
    J Med Microbiol, 1999 Jul;48(7):657-662.
    PMID: 10403416 DOI: 10.1099/00222615-48-7-657
    PCR and restriction fragment length polymorphism (RFLP) typing of flagellin genes (fliC) from 57 clinical isolates of Burkholderia cepacia indicated that only type 11 flagellins were present. Twenty-two isolates previously identified as the epidemic UK cystic fibrosis strain were indistinguishable by this method, as were 11 isolates from a pseudo-outbreak in Senegal. Other clinical isolates, including 19 from disparate sources in Malaysia, were separated into nine fliC RFLP groups, exhibiting a large degree of divergence. When isolates were indistinguishable by fliC genotyping, their similarity was confirmed by whole genome macro-restriction analysis with pulsed-field gel electrophoresis following XbaI digestion. The variation in fliC sequences of B. cepacia was far greater than that with B. pseudomallei, supporting the view that 'B. cepacia', as currently defined, may comprise several different genomic species.
    MeSH terms: Cystic Fibrosis/complications*; Electrophoresis, Agar Gel; Flagellin/genetics*; Flagellin/chemistry; Great Britain; Humans; Image Processing, Computer-Assisted; Malaysia; Phylogeny; Polymorphism, Restriction Fragment Length; RNA, Bacterial/chemistry; Senegal; Genetic Variation/genetics*; Deoxyribonucleases, Type II Site-Specific/chemistry; Polymerase Chain Reaction; Electrophoresis, Gel, Pulsed-Field; Burkholderia cepacia/classification*; Burkholderia cepacia/genetics; Burkholderia cepacia/chemistry; DNA Primers/chemistry; Burkholderia Infections/complications*
  18. Cox-Singh J, Pomrehn AS, Rahman HA, Zakaria R, Miller AO, Singh B
    Int J Parasitol, 1999 May;29(5):717-21.
    PMID: 10404266
    In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative.
    MeSH terms: Animals; Elephantiasis, Filarial/blood; Elephantiasis, Filarial/epidemiology; Elephantiasis, Filarial/parasitology*; False Negative Reactions; Humans; Malaysia/epidemiology; Sensitivity and Specificity; Polymerase Chain Reaction/methods*; Brugia malayi/genetics; Brugia malayi/isolation & purification*; DNA, Helminth/isolation & purification*
  19. Lee WS, Boey CC
    J Paediatr Child Health, 1999 Jun;35(3):260-3.
    PMID: 10404446
    OBJECTIVES: To review the causes, clinical features and outcomes of Malaysian children who had chronic diarrhoea.

    METHODOLOGY: A prospective study was performed on children with diarrhoea of more than 14 days' duration who were managed at the Department of Paediatrics, University of Malaya Medical Centre, Kuala Lumpur from 1 January 1996 to 31 December 1997.

    RESULTS: Twenty-seven patients (14 boys and 13 girls) were studied. The median age of onset of diarrhoea was 6 months and the mean duration of symptoms before referral was 66.5 days. The underlying causes of diarrhoea were found to be: (i) prolonged diarrhoea due to well-defined entities (intestinal lymphangiectasia, two cases; congenital glucose-galactose malabsorption, one case; post-small bowel resection, one case; (ii) postenteritis diarrhoea (cow's milk protein intolerance, eight cases; secondary lactose intolerance, four cases; transient monosaccharide intolerance, one case; (iii) gastrointestinal infections (nontyphoid Salmonella gastroenteritis, three cases; trichuriasis, two cases; amoebiasis, one case; adenovirus, one case; (iv) cases in which a firm diagnosis could not be established (three cases). The mean duration of hospital admission was 63 days. Sixteen cases required a change in diet, while nine cases required total parenteral nutrition. One death occurred.

    CONCLUSIONS: Chronic childhood diarrhoea in Malaysia had a variety of aetiologies. A specific diagnosis could be established in 90% of cases. Making a diagnosis was important because this led to appropriate therapy and a good outcome in 96% of cases.

    MeSH terms: Child, Preschool; Chronic Disease; Female; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male; Prospective Studies; Treatment Outcome; Age of Onset
  20. Chan PW, Goh AY, Chua KB, Kharullah NS, Hooi PS
    J Paediatr Child Health, 1999 Jun;35(3):287-90.
    PMID: 10404452
    OBJECTIVE: To study the viral aetiology of lower respiratory tract infection (LRTI) in young Malaysian children.

    METHODOLOGY: A retrospective review was performed of LRTI patients aged less than 24 months who were admitted to the University Malaya Medical Centre between 1982 and 1997. Respiratory viruses in their nasopharyngeal secretion were identified by indirect immunofluorescence, viral culture, or both.

    RESULTS: A total of 5691 children were included in the study. The mean age was 8.6 +/- 6.6 months and the M:F ratio was 1.6:1. The most common diagnosis was pneumonia (52%) followed by bronchiolitis (45%) and croup (2%). Positive viral isolation rate was 22.0%. Respiratory syncytial virus (RSV) was the commonest virus isolated (84%), followed by parainfluenza virus (8%), influenza virus (6%) and adenovirus (2%). Patients with positive virus isolation were younger (7.8 +/- 6.2 vs 8.7 +/- 6.7 months, P = 0.0001) and were more likely to have bronchiolitis.

    CONCLUSION: Young Malaysian children admitted with LRTI had a 22% viral isolation rate and RSV was the commonest virus isolated.

    MeSH terms: Bronchiolitis/epidemiology*; Bronchiolitis/virology*; Croup/epidemiology*; Croup/virology*; Female; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male; Pneumonia, Viral/epidemiology*; Retrospective Studies
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