Browse publications by year: 2014

  1. Mohd-Zaki AH, Brett J, Ismail E, L'Azou M
    PLoS Negl Trop Dis, 2014;8(11):e3159.
    PMID: 25375211 DOI: 10.1371/journal.pntd.0003159
    A literature survey and analysis was conducted to describe the epidemiology of dengue disease in Malaysia between 2000 and 2012. Published literature was searched for epidemiological studies of dengue disease, using specific search strategies for each electronic database; 237 relevant data sources were identified, 28 of which fulfilled the inclusion criteria. The epidemiology of dengue disease in Malaysia was characterized by a non-linear increase in the number of reported cases from 7,103 in 2000 to 46,171 in 2010, and a shift in the age range predominance from children toward adults. The overall increase in dengue disease was accompanied by a rise in the number, but not the proportion, of severe cases. The dominant circulating dengue virus serotypes changed continually over the decade and differed between states. Several gaps in epidemiological knowledge were identified; in particular, studies of regional differences, age-stratified seroprevalence, and hospital admissions.

    PROTOCOL REGISTRATION: PROSPERO #CRD42012002293.

    MeSH terms: Dengue/epidemiology*; Dengue Virus/classification*; Dengue Virus/immunology; Humans; Malaysia/epidemiology; Seasons; Socioeconomic Factors; Seroepidemiologic Studies; Age Distribution; Sex Distribution; Serogroup
  2. Yeap LL, Lo YL
    PLoS One, 2014;9(11):e111544.
    PMID: 25375249 DOI: 10.1371/journal.pone.0111544
    A simple liquid chromatography tandem mass spectrometry method was developed and validated according to the guidelines of the US Food and Drug Administration and the European Medicines Agency for a simultaneous quantification of levetiracetam (LEV) and its metabolite, UCB L057 in the plasma of patients. A 0.050 mL plasma sample was prepared by a simple and direct protein precipitation with 0.450 mL acetonitrile (ACN) containing 1 µg/mL of internal standard (IS, diphenhydramine), then vortex mixed and centrifuged. A 0.100 mL of the clear supernatant was diluted with 0.400 mL water and well mixed. A 0.010 mL of the resultant solution was injected into an Agilent Zorbax SB-C18 (2.1 mm×100 mm, 3.5 µm) column with an isocratic elution at 0.5 mL/min using a mixture of 0.1% formic acid in water and ACN (40:60 v/v). Detection was performed using an AB Sciex API 3000 triple quadrupole mass spectrometer, equipped with a Turbo Ion Spray source, operating in a positive mode: LEV at transition 171.1>154.1, UCB L057 at 172.5>126.1, and IS at 256.3>167.3; with an assay run time of 2 minutes. The lower limit of quantification (LLOQ) for both LEV and UCB L057 was validated at 0.5 µg/mL, while their lower limit of detection (LOD) was 0.25 µg/mL. The calibration curves were linear between 0.5 and 100 µg/mL for both analytes. The inaccuracy and imprecision of both intra-assay and inter-assay were less than 10%. Matrix effects were consistent between sources of plasma and the recoveries of all compounds were between 100% and 110%. Stability was established under various storage and processing conditions. The carryovers from both LEV and UCB L057 were less than 6% of the LLOQ and 0.13% of the IS. This assay method has been successfully applied to a population pharmacokinetic study of LEV in patients with epilepsy.
    MeSH terms: Chromatography, Liquid*; Epilepsy/blood; Humans; Piracetam/analogs & derivatives*; Piracetam/blood; Piracetam/metabolism; Reproducibility of Results; Tandem Mass Spectrometry*; Limit of Detection
  3. Ganasegeran K, Rajendran AK, Al-Dubai SA
    PLoS One, 2014;9(11):e112124.
    PMID: 25375256 DOI: 10.1371/journal.pone.0112124
    The use of complementary and alternative medicine (CAM) as a source of cure has gained much spectrum worldwide, despite skeptics and advocates of evidence-based practice conceptualized such therapies as human nostrum.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Complementary Therapies/psychology*; Complementary Therapies/utilization; Cross-Sectional Studies; Female; Humans; Income; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Rural Population; Socioeconomic Factors; Logistic Models; Young Adult
  4. Hasima N, Ozpolat B
    Cell Death Dis, 2014;5:e1509.
    PMID: 25375374 DOI: 10.1038/cddis.2014.467
    Autophagy, a lysosomal degradation pathway for cellular constituents and organelles, is an adaptive and essential process required for cellular homeostasis. Although autophagy functions as a survival mechanism in response to cellular stressors such as nutrient or growth factor deprivation, it can also lead to a non-apoptotic form of programmed cell death (PCD) called autophagy-induced cell death or autophagy-associated cell death (type II PCD). Current evidence suggests that cell death through autophagy can be induced as an alternative to apoptosis (type I PCD), with therapeutic purpose in cancer cells that are resistant to apoptosis. Thus, modulating autophagy is of great interest in cancer research and therapy. Natural polyphenolic compounds that are present in our diet, such as rottlerin, genistein, quercetin, curcumin, and resveratrol, can trigger type II PCD via various mechanisms through the canonical (Beclin-1 dependent) and non-canonical (Beclin-1 independent) routes of autophagy. The capacity of these compounds to provide a means of cancer cell death that enhances the effects of standard therapies should be taken into consideration for designing novel therapeutic strategies. This review focuses on the autophagy- and cell death-inducing effects of these polyphenolic compounds in cancer.
    MeSH terms: Acetophenones/therapeutic use; Antineoplastic Agents, Phytogenic/therapeutic use*; Autophagy/drug effects; Autophagy/genetics; Benzopyrans/therapeutic use; Curcumin/therapeutic use; Humans; Membrane Proteins/genetics; Membrane Proteins/metabolism; Neoplasms/diet therapy*; Neoplasms/genetics; Neoplasms/metabolism; Neoplasms/pathology; Quercetin/therapeutic use; Stilbenes/therapeutic use; Signal Transduction; Gene Expression Regulation, Neoplastic*; Apoptosis/genetics; Genistein/therapeutic use; Cell Line, Tumor; Apoptosis Regulatory Proteins/genetics; Apoptosis Regulatory Proteins/metabolism; Polyphenols/therapeutic use*
  5. Fernando HA, Chandramouli C, Rosli D, Lam YL, Yong ST, Yaw HP, et al.
    Nutrients, 2014 Nov 04;6(11):4856-71.
    PMID: 25375630 DOI: 10.3390/nu6114856
    Glycyrrhizic acid (GA) ameliorates many components of the metabolic syndrome, but its potential therapeutic use is marred by edema caused by inhibition of renal 11β-hydroxysteroid dehydrogenase 2 (11β-HSD2). We assessed whether 100 mg/kg per day GA administered orally could promote metabolic benefits without causing edema in rats fed on a high-sucrose diet. Groups of eight male rats were fed on one of three diets for 28 days: normal diet, a high-sucrose diet, or a high-sucrose diet supplemented with GA. Rats were then culled and renal 11β-HSD2 activity, as well as serum sodium, potassium, angiotensin II and leptin levels were determined. Histological analyses were performed to assess changes in adipocyte size in visceral and subcutaneous depots, as well as hepatic and renal tissue morphology. This dosing paradigm of GA attenuated the increases in serum leptin levels and visceral, but not subcutaneous adipocyte size caused by the high-sucrose diet. Although GA decreased renal 11β-HSD2 activity, it did not affect serum electrolyte or angiotensin II levels, indicating no onset of edema. Furthermore, there were no apparent morphological changes in the liver or kidney, indicating no toxicity. In conclusion, it is possible to reap metabolic benefits of GA without edema using the current dosage and treatment time.
    MeSH terms: Administration, Oral; Angiotensin II/blood; Animals; Edema/chemically induced*; Kidney/drug effects; Kidney/enzymology; Liver/drug effects; Liver/metabolism; Male; Potassium/blood; Sodium/blood; Rats, Sprague-Dawley; Dietary Sucrose/administration & dosage*; Dietary Sucrose/adverse effects; Glycyrrhizic Acid/pharmacology*; Leptin/blood; Metabolic Syndrome X/drug therapy; 11-beta-Hydroxysteroid Dehydrogenase Type 2/antagonists & inhibitors; 11-beta-Hydroxysteroid Dehydrogenase Type 2/metabolism; Rats
  6. Ismail IH, Boyle RJ, Mah LJ, Licciardi PV, Tang ML
    Pediatr Allergy Immunol, 2014 Nov;25(7):674-84.
    PMID: 25376403 DOI: 10.1111/pai.12303
    Regulatory T cells (Treg) play an essential role in early immune programming and shaping the immune response towards a pro-allergic or tolerant state. We evaluated cord blood Treg and cytokine responses to microbial and non-microbial stimuli in infants at high risk of allergic disease and their associations with development of allergic disease in the first year.
    MeSH terms: Antigens, Bacterial/immunology; Cells, Cultured; Eczema/immunology*; Female; Fetal Blood/immunology; Follow-Up Studies; Humans; Immune Tolerance; Infant; Infant, Newborn; Lipopolysaccharides/immunology; Male; Risk; Teichoic Acids/immunology; Cytokines/metabolism; T-Lymphocytes, Regulatory/immunology*; Toll-Like Receptor 2/immunology; Toll-Like Receptor 4; Forkhead Transcription Factors/metabolism; Lactobacillus rhamnosus/immunology*; Interleukin-2 Receptor alpha Subunit/metabolism
  7. McDonald SA, Mohamed R, Dahlui M, Naning H, Kamarulzaman A
    BMC Infect Dis, 2014;14:564.
    PMID: 25377240 DOI: 10.1186/s12879-014-0564-6
    Collecting adequate information on key epidemiological indicators is a prerequisite to informing a public health response to reduce the impact of hepatitis C virus (HCV) infection in Malaysia. Our goal was to overcome the acute data shortage typical of low/middle income countries using statistical modelling to estimate the national HCV prevalence and the distribution over transmission pathways as of the end of 2009.
    MeSH terms: Adolescent; Adult; Ethnic Groups; Female; Hepatitis C/epidemiology*; Hepatitis C/prevention & control; Hepatitis C/transmission; Humans; Malaysia/epidemiology; Male; Middle Aged; Risk Factors; Models, Statistical; Substance Abuse, Intravenous/epidemiology; Prevalence
  8. Gan HY, Gan HM, Tarasco AM, Busairi NI, Barton HA, Hudson AO, et al.
    Genome Announc, 2014;2(6).
    PMID: 25377711 DOI: 10.1128/genomeA.01133-14
    Here, we report the whole-genome sequences and annotation of five oligotrophic bacteria from two sites within the Lechuguilla Cave in the Carlsbad Caverns National Park, NM. Three of the five genomes contain an acyl-homoserine lactone signal synthase ortholog (luxI) that is involved in cell-to-cell communication via quorum sensing.
    MeSH terms: Parks, Recreational; Bacteria; Cell Communication; 4-Butyrolactone; Quorum Sensing; Acyl-Butyrolactones
  9. Carragher N, Byrnes J, Doran CM, Shakeshaft A
    Bull World Health Organ, 2014 Oct 01;92(10):726-33.
    PMID: 25378726 DOI: 10.2471/BLT.13.130708
    OBJECTIVE: To demonstrate the development and feasibility of a tool to assess the adequacy of national policies aimed at reducing alcohol consumption and related problems.

    METHODS: We developed a quantitative tool - the Toolkit for Evaluating Alcohol policy Stringency and Enforcement (TEASE-16) - to assess the level of stringency and enforcement of 16 alcohol control policies. TEASE-16 was applied to policy data from nine study areas in the western Pacific: Australia, China excluding Hong Kong Special Administrative Region (SAR), Hong Kong SAR, Japan, Malaysia, New Zealand, the Philippines, Singapore and Viet Nam. Correlation and regression analyses were then used to examine the relationship between alcohol policy scores and income-adjusted levels of alcohol consumption per capita.

    FINDINGS: Vast differences exist in how alcohol control policies are implemented in the western Pacific. Out of a possible 100 points, the nine study areas achieved TEASE-16 scores that ranged from 24.1 points for the Philippines to 67.5 points for Australia. Study areas with high policy scores - indicating relatively strong alcohol policy frameworks - had lower alcohol consumption per capita. Sensitivity analyses indicated scores and rankings for each study area remained relatively stable across different weighting schemes, indicating that TEASE-16 was robust.

    CONCLUSION: TEASE-16 could be used by international and national regulatory bodies and policy-makers to guide the design, implementation, evaluation and refinement of effective policies to reduce alcohol consumption and related problems.

    MeSH terms: Alcohol Drinking/legislation & jurisprudence*; Alcohol Drinking/prevention & control*; Asia; Australia; Feasibility Studies; Health Policy*; Humans; Income/statistics & numerical data; New Zealand
  10. Moghadamtousi SZ, Rouhollahi E, Karimian H, Fadaeinasab M, Abdulla MA, Kadir HA
    Drug Des Devel Ther, 2014;8:2099-110.
    PMID: 25378912 DOI: 10.2147/DDDT.S70096
    The popular fruit tree of Annona muricata L. (Annonaceae), known as soursop and graviola, is a widely distributed plant in Central and South America and tropical countries. Leaves of A. muricata have been reported to possess antioxidant and anti-inflammatory activities. In this study, the gastroprotective effects of ethyl acetate extract of A. muricata leaves (EEAM) were investigated against ethanol-induced gastric injury models in rats. The acute toxicity test of EEAM in rats, carried out in two doses of 1 g/kg and 2 g/kg, showed the safety of this plant, even at the highest dose of 2 g/kg. The antiulcer study in rats (five groups, n=6) was performed with two doses of EEAM (200 mg/kg and 400 mg/kg) and with omeprazole (20 mg/kg), as a standard antiulcer drug. Gross and histological features showed the antiulcerogenic characterizations of EEAM. There was significant suppression on the ulcer lesion index of rats pretreated with EEAM, which was comparable to the omeprazole effect in the omeprazole control group. Oral administration of EEAM to rats caused a significant increase in the level of nitric oxide and antioxidant activities, including catalase, glutathione, and superoxide dismutase associated with attenuation in gastric acidity, and compensatory effect on the loss of gastric wall mucus. In addition, pretreatment of rats with EEAM caused significant reduction in the level of malondialdehyde, as a marker for oxidative stress, associated with an increase in prostaglandin E2 activity. Immunohistochemical staining also demonstrated that EEAM induced the downregulation of Bax and upregulation of Hsp70 proteins after pretreatment. Collectively, the present results suggest that EEAM has a promising antiulcer potential, which could be attributed to its suppressive effect against oxidative damage and preservative effect toward gastric wall mucus.
    MeSH terms: Ethanol/adverse effects; Animals; Anti-Ulcer Agents/isolation & purification; Anti-Ulcer Agents/therapeutic use*; Anti-Ulcer Agents/chemistry; Female; Male; Plant Extracts/isolation & purification; Plant Extracts/therapeutic use*; Plant Extracts/chemistry; Stomach Ulcer/chemically induced; Stomach Ulcer/metabolism; Stomach Ulcer/pathology; Stomach Ulcer/prevention & control*; Rats, Sprague-Dawley; Plant Leaves/chemistry*; HSP70 Heat-Shock Proteins/metabolism*; Annona/chemistry*; bcl-2-Associated X Protein/metabolism*; Rats
  11. Al Jumah K, Hassali MA, Al Qhatani D, El Tahir K
    Neuropsychiatr Dis Treat, 2014;10:2031-7.
    PMID: 25378929 DOI: 10.2147/NDT.S71697
    Several studies have investigated the factors associated with adherence to antidepressants, with inconsistent conclusions. However, no similar study has investigated this issue among patients diagnosed with major depressive disorder in Saudi Arabia. The aim of this study is to explore patients' adherence to antidepressant medications, and the factors associated with adherence.
    MeSH terms: Antidepressive Agents; Depressive Disorder, Major; Humans; Patient Compliance; Saudi Arabia
  12. Aziz CB, Ismail CA, Hussin CM, Mohamed M
    J Tradit Complement Med, 2014 Oct;4(4):298-302.
    PMID: 25379476 DOI: 10.4103/2225-4110.139115
    Tualang honey ( Fēng Mì) is known to have anti-inflammatory property, but its antinociceptive property has not been extensively investigated. In this study, we examined the preemptive effects on administering different doses of Tualang honey and prednisolone on the nociceptive response in male Sprague-Dawley rats. Thirty-five male Sprague-Dawley rats were randomized into five groups (n = 7) and each group received either distilled water, Tualang honey (0.2, 1.2 or 2.4 g/kg) or prednisolone (10 mg/kg) for 10 days. The response to noxious thermal stimulus was assessed using tail flick test on Day 10. The well-being of the rats was also assessed by monitoring their food intake and body weight. Data were analyzed using one-way Analysis of Variance (ANOVA) with post-hoc Scheffe's test and P value less than 0.05 was considered significant. In tail flick test, the tail flick latency time was significantly higher in the groups that received 1.2 g/kg and 2.4 g/kg of Tualang honey and 10 mg/kg of prednisolone, compared to the control group (P < 0.05). There was significant reduction in the total food pellet intake in the groups receiving prednisolone and Tualang honey (1.2 g/kg and 2.4 g/kg) compared to controls; however, the body weight gain was only significantly reduced in the prednisolone group. All the parameters were not significantly affected in the group receiving 0.2 g/kg of Tualang honey. In conclusion, preemptive administration of Tualang honey (1.2 g/kg and 2.4 g/kg) and prednisolone (10 mg/kg) had reduced the pain responses. The reduced weight gain in the prednisolone group is an unwanted effect due to its metabolic and central actions. Further studies are required to confirm the antinociceptive effects and elucidate the mechanism of antinociceptive action of Tualang honey in the rats.
  13. Khosravi Y, Vellasamy KM, Mariappan V, Ng SL, Vadivelu J
    ScientificWorldJournal, 2014;2014:132971.
    PMID: 25379514 DOI: 10.1155/2014/132971
    Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many antibiotics. Ceftazidime (CAZ), the synthetic β-lactam, is normally used as the first-line antibiotic therapy for treatment of melioidosis. However, acquired CAZ resistance can develop in vivo during treatment with CAZ, leading to mortality if therapy is not switched to a different antibiotic(s) in a timely manner. In this study, susceptibilities of 81 B. pseudomallei isolates to nine different antimicrobial agents were determined using the disk diffusion method, broth microdilution test and Etest. Highest percentage of susceptibility was demonstrated to CAZ, amoxicillin/clavulanic acid, meropenem, imipenem, and trimethoprim/sulfamethoxazole. Although these drugs demonstrated the highest percentage of susceptibility in B. pseudomallei, the overall results underline the importance of the emergence of resistance in this organism. PCR results showed that, of the 81 B. pseudomallei, six multidrug resistant (MDR) isolates carried bpeB, amrB, and BPSS1119 and penA genes. Genotyping of the isolates using random amplified polymorphic DNA analysis showed six different PCR fingerprinting patterns generated from the six MDR isolates clusters (A) and eight PCR fingerprinting patterns generated for the remaining 75 non-MDR isolates clusters (B).
    MeSH terms: Amoxicillin/pharmacology; Anti-Bacterial Agents/pharmacology*; Bacterial Proteins/genetics*; Bacterial Proteins/metabolism; Humans; Malaysia; Melioidosis/drug therapy; Melioidosis/microbiology; Microbial Sensitivity Tests; Thienamycins/pharmacology; Imipenem/pharmacology; Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology; Gene Expression; DNA Fingerprinting; Burkholderia pseudomallei/drug effects*; Burkholderia pseudomallei/genetics*; Burkholderia pseudomallei/growth & development; Clavulanic Acid/pharmacology; Drug Resistance, Bacterial; Membrane Transport Proteins/genetics*; Membrane Transport Proteins/metabolism
  14. Hindia MN, Reza AW, Noordin KA
    ScientificWorldJournal, 2014;2014:246206.
    PMID: 25379524 DOI: 10.1155/2014/246206
    Nowadays, one of the most important challenges in heterogeneous networks is the connection consistency between the mobile station and the base stations. Furthermore, along the roaming process between the mobile station and the base station, the system performance degrades significantly due to the interferences from neighboring base stations, handovers to inaccurate base station and inappropriate technology selection. In this paper, several algorithms are proposed to improve mobile station performance and seamless mobility across the long-term evolution (LTE) and Worldwide Interoperability for Microwave Access (WiMAX) technologies, along with a minimum number of redundant handovers. Firstly, the enhanced global positioning system (GPS) and the novel received signal strength (RSS) prediction approaches are suggested to predict the target base station accurately. Then, the multiple criteria with two thresholds algorithm is proposed to prioritize the selection between LTE and WiMAX as the target technology. In addition, this study also covers the intercell and cochannel interference reduction by adjusting the frequency reuse ratio 3 (FRR3) to work with LTE and WiMAX. The obtained results demonstrate high next base station prediction efficiency and high accuracy for both horizontal and vertical handovers. Moreover, the received signal strength is kept at levels higher than the threshold, while maintaining low connection cost and delay within acceptable levels. In order to highlight the combination of the proposed algorithms' performance, it is compared with the existing RSS and multiple criteria handover decision algorithms.
    MeSH terms: Algorithms*; Humans; Microwaves; Signal Processing, Computer-Assisted/instrumentation*; Geographic Information Systems; Wireless Technology/instrumentation*
  15. Safaei MR, Mahian O, Garoosi F, Hooman K, Karimipour A, Kazi SN, et al.
    ScientificWorldJournal, 2014;2014:740578.
    PMID: 25379542 DOI: 10.1155/2014/740578
    This paper addresses erosion prediction in 3-D, 90° elbow for two-phase (solid and liquid) turbulent flow with low volume fraction of copper. For a range of particle sizes from 10 nm to 100 microns and particle volume fractions from 0.00 to 0.04, the simulations were performed for the velocity range of 5-20 m/s. The 3-D governing differential equations were discretized using finite volume method. The influences of size and concentration of micro- and nanoparticles, shear forces, and turbulence on erosion behavior of fluid flow were studied. The model predictions are compared with the earlier studies and a good agreement is found. The results indicate that the erosion rate is directly dependent on particles' size and volume fraction as well as flow velocity. It has been observed that the maximum pressure has direct relationship with the particle volume fraction and velocity but has a reverse relationship with the particle diameter. It also has been noted that there is a threshold velocity as well as a threshold particle size, beyond which significant erosion effects kick in. The average friction factor is independent of the particle size and volume fraction at a given fluid velocity but increases with the increase of inlet velocities.
    MeSH terms: Computer Simulation; Copper/chemistry; Particle Size; Rheology; Models, Statistical*; Nanoparticles/chemistry*
  16. Ramli NS, Ismail P, Rahmat A
    ScientificWorldJournal, 2014;2014:964731.
    PMID: 25379555 DOI: 10.1155/2014/964731
    The aim of this study was to examine the effects of extraction methods on antioxidant capacities of red dragon fruit peel and flesh. Antioxidant capacities were measured using ethylenebenzothiozoline-6-sulfonic acid (ABTS) radical cation assay and ferric reducing antioxidant power assay (FRAP). Total phenolic content (TPC) was determined using Folin-Ciocalteu reagent while quantitative determination of total flavonoid content (TFC) was conducted using aluminium trichloride colorimetric method. Betacyanin content (BC) was measured by spectrophotometer. Red dragon fruit was extracted using conventional (CV) and ultrasonic-assisted extraction (UE) technique to determine the most efficient way of extracting its antioxidant components. Results indicated that UE increased TFC, reduced the extraction yield, BC, and TPC, but exhibited the strongest scavenging activity for the peel of red dragon fruit. In contrast, UE reduced BC, TFC, and scavenging activity but increased the yield for the flesh. Nonetheless, UE slightly increases TPC in flesh. Scavenging activity and reducing power were highly correlated with phenolic and flavonoid compounds. Conversely, the scavenging activity and reducing power were weakly correlated with betacyanin content. This work gives scientific evidences for the consideration of the type of extraction techniques for the peel and flesh of red dragon fruit in applied research and food industry.
    MeSH terms: Antioxidants/isolation & purification*; Antioxidants/chemistry; Flavonoids/isolation & purification*; Flavonoids/chemistry; Fruit/chemistry*; Phenols/isolation & purification*; Phenols/chemistry; Plant Extracts/chemistry; Sonication; Sulfonic Acids/chemistry; Cactaceae/chemistry*; Betacyanins/isolation & purification*; Betacyanins/chemistry; Benzothiazoles/chemistry; Solid Phase Extraction/methods
  17. Nordin N, Salama SM, Golbabapour S, Hajrezaie M, Hassandarvish P, Kamalidehghan B, et al.
    PLoS One, 2014;9(11):e111925.
    PMID: 25379712 DOI: 10.1371/journal.pone.0111925
    A natural source of medicine, Enicosanthellum pulchrum is a tropical plant which belongs to the family Annonaceae. In this study, methanol extract from the leaves and stems of this species was evaluated for its gastroprotective potential against mucosal lesions induced by ethanol in rats. Seven groups of rats were assigned, groups 1 and 2 were given Tween 20 (10% v/v) orally. Group 3 was administered omeprazole 20 mg/kg (10% Tween 20) whilst the remaining groups received the leaf and stem extracts at doses of 150 and 300 mg/kg, respectively. After an additional hour, the rats in groups 2-7 received ethanol (95% v/v; 8 mL/kg) orally while group 1 received Tween 20 (10% v/v) instead. Rats were sacrificed after 1 h and their stomachs subjected to further studies. Macroscopically and histologically, group 2 rats showed extremely severe disruption of the gastric mucosa compared to rats pre-treated with the E. pulchrum extracts based on the ulcer index, where remarkable protection was noticed. Meanwhile, a significant percentage of inhibition was shown with the stem extract at 62% (150 mg/kg) and 65% (300 mg/kg), whilst the percentage with the leaf extract at doses of 150 and 300 mg/kg was 63% and 75%, respectively. An increase in mucus content, nitric oxide, glutathione, prostaglandin E2, superoxide dismutase, protein and catalase, and a decrease in malondialdehyde level compared to group 2 were also obtained. Furthermore, immunohistochemical staining of groups 4-7 exhibited down-regulation of Bax and up-regulation of Hsp70 proteins. The methanol extract from the leaves and the stems showed notable gastroprotective potential against ethanol.
    MeSH terms: Ethanol/adverse effects*; Methanol/chemistry*; Animals; Antioxidants/metabolism; Catalase/metabolism; Disease Models, Animal; Gastric Mucosa/drug effects; Gastric Mucosa/metabolism; Gastric Mucosa/pathology; Glycoproteins/metabolism; Male; Malondialdehyde/metabolism; Plant Extracts/pharmacology*; Stomach Ulcer/chemically induced*; Stomach Ulcer/metabolism; Stomach Ulcer/pathology; Stomach Ulcer/prevention & control*; Dinoprostone/metabolism; Rats, Sprague-Dawley; Plant Leaves/chemistry; Plant Stems/chemistry; Annonaceae/chemistry*; Rats
  18. Naing C, Aung K
    Medicine (Baltimore), 2014 Nov;93(21):e100.
    PMID: 25380081 DOI: 10.1097/MD.0000000000000100
    Hypertension (HPT) is the most common condition seen in primary care that can lead to health consequences and death if not detected early and treated appropriately.This study aimed to synthesize the prevalence, awareness, and control of HPT, and investigate the risk factors for HPT in Myanmar.We performed a meta-analysis of observational studies. Relevant studies were searched in electronic databases. The methodological quality of the included studies was assessed in 3 domains: selection bias, measurement bias, and bias related to data analysis. The overall prevalence and proportions was calculated using random-effect model of DerSimonian-Laird method. To identify the risk factors for HPT in Myanmar, we entered the ratio measures of the (adjusted) effect as a log odds ratio (OR) and the standard error of the log OR using generic inverse-variance weighting method. For stability of results, we performed leave-one-study-out sensitivity analysis by omitting individual studies one at a time from the meta-analysis.Seven studies (n = 20,901) were included in this analysis. Overall prevalence of HPT in Myanmar was 22% (95% confidence interval (CI): 14%-31.7%, I: 99.6%), stratified as 21.5% (95% CI: 14.1%-29.9%, I: 98.7%) in men and 22.7% (95% CI: 10.8%-34.6%, I: 99.5%) in women. Overall, prevalence of HPT increased with an advancing age of the participants. The proportions of awareness and controlled HPT were 55% (95% CI: 43%-67%, I: 97.7%) and 11% (95% CI: 6%-15%, I: 93.8%), respectively. A weak but significant association was observed between HPT and alcohol drinking (summary OR: 1.38, 95% CI: 1.14%-1.65, I: 0%) and smoking (summary OR: 1.32, 95% CI: 1.0%-1.74, I: 50%). In sensitivity analysis, when a study that made confirmation of HPT by the former World Health Organization criteria was dropped, the prevalence increased to 26% (95% CI: 20.8%-32.1%, I: 98.1%).HPT was considerably prevalent in Myanmar, while the levels of awareness and controlled HPT were low. Health promotion strategy tailored to the education on modifiable risk factors and establishment of blood pressure screening in primary health care context would be of immense value. Upcoming well-powered studies, using the standardized research design and covering more regions of the country are recommended.
    MeSH terms: Adolescent; Adult; Aged; Blood Pressure Determination; Myanmar/epidemiology; Female; Health Services Needs and Demand; Humans; Male; Risk Factors; Prevalence; Disease Management; Observational Studies as Topic
  19. Hussein MZ, Jaafar AM, Yahaya AH, Masarudin MJ, Zainal Z
    Int J Mol Sci, 2014;15(11):20254-65.
    PMID: 25380526 DOI: 10.3390/ijms151120254
    Multi-walled carbon nanotubes (MWCNTs) were prepared via chemical vapor deposition (CVD) using a series of different catalysts, derived from FeCoNiAl, CoNiAl and FeNiAl layered double hydroxides (LDHs). Catalyst-active particles were obtained by calcination of LDHs at 800 °C for 5 h. Nitrogen and hexane were used as the carrier gas and carbon source respectively, for preparation of MWCNTs using CVD methods at 800 °C. MWCNTs were allowed to grow for 30 min on the catalyst spread on an alumina boat in a quartz tube. The materials were subsequently characterized through X-ray diffraction, Fourier transform infrared spectroscopy, surface area analysis, field emission scanning electron microscopy and transmission electron microscopy. It was determined that size and yield of MWCNTs varied depending on the type of LDH catalyst precursor that is used during synthesis. MWCNTs obtained using CoNiAl-LDH as the catalyst precursor showed smaller diameter and higher yield compared to FeCoNiAl and FeNiAl LDHs.
    MeSH terms: Adsorption; Catalysis; Hydroxides/chemistry*; Metals/chemistry*; Powders; Surface Properties; Temperature; X-Ray Diffraction; Porosity; Spectroscopy, Fourier Transform Infrared; Nanotechnology/methods*; Nanotubes, Carbon/ultrastructure; Nanotubes, Carbon/chemistry*
  20. Tay ST, Lim SL, Tan HW
    PMID: 25380692 DOI: 10.1186/1472-6882-14-439
    The increasing resistance of Candida yeasts towards antifungal compounds and the limited choice of therapeutic drugs have spurred great interest amongst the scientific community to search for alternative anti-Candida compounds. Mycocins and fungal metabolites have been reported to have the potential for treatment of fungal infections. In this study, the growth inhibition of Candida species by a mycocin produced by Wickerhamomyces anomalus and a lactone compound from Aureobasidium pullulans were investigated.
    MeSH terms: Antifungal Agents/isolation & purification; Antifungal Agents/pharmacology*; Ascomycota/chemistry*; Biological Products/isolation & purification; Biological Products/pharmacology*; Candida/drug effects*; Candida/growth & development; Fatty Acids, Monounsaturated/isolation & purification; Fatty Acids, Monounsaturated/pharmacology*; Humans; Lactones/isolation & purification; Lactones/pharmacology*; Microbial Sensitivity Tests; Mycotoxins/isolation & purification; Mycotoxins/pharmacology*; Species Specificity; Biofilms/drug effects
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