Browse publications by year: 2019

  1. Rupani PF, Delarestaghi RM, Abbaspour M, Rupani MM, El-Mesery HS, Shao W
    Environ Sci Pollut Res Int, 2019 Nov;26(32):32777-32789.
    PMID: 31520387 DOI: 10.1007/s11356-019-06456-5
    By growing urban population, Iran faces numerous environmental issues and solid waste management is on the top of these problems. Studies showed that a daily average of 700-1000 g of wastes are produced per person in Iran, in which organic waste accounts for a significant amount. On the other hand, hospital waste represents a part of the wastes, which need careful consideration from the environmental point of view. In the present study, the amount, composition, and management of urban and hospital wastes were evaluated in 7 Iranian metropolises, which account for about 30% of the population and produce about 35% of the country wastes. Based on prior surveys, landfill method is the current main method for waste management in these cities, which is generally not completely sanitary and therefore causes many environmental problems. The other common methods for waste management in these cities are composting of organic wastes, and the use of waste conversion methods to energy. However, the latter is ongoing only in Tehran which also includes some limitations. Therefore, the study also evaluated the future perspectives and feasibility of waste-to-energy conversion as a promising economic route for waste disposal.
    MeSH terms: Cities; Feasibility Studies; Humans; Iran; Longitudinal Studies; Population Growth; Surveys and Questionnaires; Refuse Disposal; Urban Population; Waste Management; Solid Waste; Waste Disposal Facilities
  2. Nik Azis NN, Chew FLM, Rosland SF, Ramlee A, Che-Hamzah J
    J AAPOS, 2019 10;23(5):268.e1-268.e6.
    PMID: 31520719 DOI: 10.1016/j.jaapos.2019.01.019
    PURPOSE: To evaluate parents' performance in using the American Association for Pediatric Ophthalmology and Strabismus (AAPOS) Vision Screening App (application) as a vision screening tool among preschool children and to evaluate the reliability of this app.

    METHODS: A total of 195 5- and 6-year-old preschoolers were recruited from children attending Hospital Selayang, Selangor, Malaysia, to test the app. Uncooperative children and those with visual acuity of >logMAR 0.6 were excluded. Results from parents and the screening doctor using the app (Lea symbols) to test visual acuity were compared to each other and to gold standard vision testing by an optometrist using the Lea symbols chart.

    RESULTS: Children 5 years of age represented 46.7% of the study population. The mean age of parents was 37.27 ± 7.68 years. Bland-Altman scatterplot agreement between assessors mainly was within the 95% confidence interval for bilateral eyes screening. Parents obtained a sensitivity of 86.6% (right vision) and 79.5% (left vision) and specificity of 78.9% (right vision) and 71.8% (left vision). Parents took a mean of 191.2 ± 70.82 seconds for bilateral screening. The intraclass correlation coefficient between optometrist and parents in bilateral eyes screening was good (P 0.7, indicating high internal reliability of the app. Most parents (178/195 [91.3%]) strongly agreed on the app's acceptability and ease of use.

    CONCLUSIONS: The AAPOS Vision Screening App used by parents is a promising tool for visual acuity screening among Malaysian preschool children and a reliable app for vision screening.

    MeSH terms: Adult; Amblyopia/diagnosis; Amblyopia/ethnology; Child; Child, Preschool; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Ophthalmology/organization & administration; Parents*; Societies, Medical/organization & administration; Strabismus/diagnosis; Strabismus/ethnology; Visual Acuity/physiology*; Reproducibility of Results; Vision Screening/instrumentation*; Asian Continental Ancestry Group/ethnology*; Mobile Applications
  3. Islam MA, Khandker SS, Alam SS, Kotyla P, Hassan R
    Autoimmun Rev, 2019 Sep 11.
    PMID: 31520805 DOI: 10.1016/j.autrev.2019.102392
    BACKGROUND: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease where chronic inflammation and tissue or organ damage is observed. Due to various suspected causes, inadequate levels of vitamin D (a steroid hormone with immunomodulatory effects) has been reported in patients with SLE, however, contradictory.

    AIMS: The aim of this systematic review and meta-analysis was to evaluate the serum levels of vitamin D in patients with SLE in compared to healthy controls.

    METHODS: PubMed, SCOPUS, ScienceDirect and Google Scholar electronic databases were searched systematically without restricting the languages and year (up to March 2, 2019) and studies were selected based on the inclusion criteria. Mean difference (MD) along with 95% confidence intervals (CI) were used and the analyses were carried out by using a random-effects model. Different subgroup and sensitivity analyses were conducted. Study quality was assessed by the modified Newcastle-Ottawa Scale (NOS) and publication bias was evaluated by a contour-enhanced funnel plot, Begg's and Egger's tests.

    RESULTS: We included 34 case-control studies (2265 SLE patients and 1846 healthy controls) based on the inclusion criteria. Serum levels of vitamin D was detected significantly lower in the SLE patients than that in the healthy controls (MD: -10.44, 95% CI: -13.85 to -7.03; p 

    MeSH terms: Adrenal Cortex Hormones; Africa; Asia; Cholestanes; Humans; Hydroxychloroquine; Immunosuppressive Agents; Inflammation; Language; Lupus Erythematosus, Systemic; Seasons; South America; Vitamin D; Vitamins; Confidence Intervals; Case-Control Studies; Publication Bias; PubMed; Healthy Volunteers
  4. Dong M, Zeng LN, Zhang Q, Yang SY, Chen LY, Najoan E, et al.
    Asian J Psychiatr, 2019 Oct;45:74-80.
    PMID: 31520884 DOI: 10.1016/j.ajp.2019.08.010
    OBJECTIVE: Regular surveys are important to monitor the use of psychotropic medications in clinical practice. This study examined the psychotropic prescription patterns in adult Asian schizophrenia patients based on the data of the Research on Asian Psychotropic Prescription (REAP) 2016 survey.

    METHODS: This cross-sectional survey across 15 Asian countries/territories collected socio-demographic and clinical data with standardized procedures between March and May 2016. The socio-demographic and clinical characteristics of the patients were recorded with a standardized questionnaire.

    RESULTS: Altogether 3,537 adult patients with schizophrenia were consecutively screened and enrolled in the survey. The mean age was 38.66 ± 11.55 years and 59.7% of the sample were male. The mean dose of antipsychotics in chlorpromazine equivalents (CPZeq) was 424 ± 376 mg/day; 31.3% and 80.8% received first- and second- generation antipsychotics, respectively and 42.6% had antipsychotic polypharmacy, 11.7% had antidepressants, 13.7% had mood stabilizers, 27.8% had benzodiazepines, and 45.6% had anticholinergics.

    CONCLUSIONS: Psychotropic prescription patterns in Asian adult patients with schizophrenia varied across countries. Regular surveys on psychotropic medications for schizophrenia are important to monitor pharmacotherapy practice in Asia.

    MeSH terms: Adult; Antidepressive Agents/therapeutic use; Asia; Benzodiazepines/therapeutic use; Cross-Sectional Studies; Female; Health Surveys; Humans; Male; Practice Patterns, Physicians'/statistics & numerical data*; Drug Prescriptions/statistics & numerical data; Schizophrenia/drug therapy*; Antipsychotic Agents/therapeutic use*; Cholinergic Antagonists/therapeutic use
  5. Linus-Lojikip S, Subramaniam V, Lim WY, Hss AS
    Complement Ther Clin Pract, 2019 Sep 06;37:73-85.
    PMID: 31521007 DOI: 10.1016/j.ctcp.2019.09.001
    BACKGROUND: This case series describes the survival outcomes of patients who underwent integrative medicine (IM) protocol for ovarian cancer, a treatment protocol, that integrated a carefully selected set of complementary and alternative medicine (CAM) into the conventional treatment for ovarian cancers.

    MATERIALS AND METHODS: Retrospective review of patients' medical records was conducted at a private medical centre that delivered the IM protocol for patients with advanced and recurrent ovarian cancers. We explored and analysed the overall survival and disease progressions of those who received the IM treatment for at least 2 months.

    RESULTS: Forty patients with advanced ovarian cancers fulfilled the inclusion criteria for this case series. An overall of 75% of the cases achieved remission with initial IM treatment, 17.5% had a partial response and 7.5% showed progressive disease. The overall 5-year survival for all 40 cases is 53.1%. When explored further, the 5-year survival for cases who received CAM only is 75%, and cases who received combined limited chemotherapy with CAM had a 5-year survival of 55%. At study endpoint, 11 cases died due to ovarian cancer.

    CONCLUSION: These findings suggest that CAM may be a valuable addition to conventional therapy to treat and improve the survival of patients with ovarian cancers. A formal randomized control trial is required to evaluate the efficacy and long-term outcomes of using IM to treat advanced and recurrent ovarian cancers.

    MeSH terms: Complementary Therapies; Clinical Protocols; Female; Forms and Records Control; Humans; Medical Records; Neoplasms, Glandular and Epithelial; Ovarian Neoplasms; Retrospective Studies; Disease Progression; Integrative Medicine
  6. Hasan UA, Mohd Hairon S, Yaacob NM, Daud A, Abdul Hamid A, Hassan N, et al.
    PMID: 31514391 DOI: 10.3390/ijerph16183356
    Background: Structured education is needed to cultivate safe sharp disposal behavior among diabetic patients. Thus, this study aimed to assess the effectiveness of the Diabetes Community Sharp Disposal Education Module in improving knowledge and sharp disposal practice among Malaysian Type 2 diabetic patients. Methods: This quasi-experimental study was conducted at primary health clinics in two districts in Kelantan, a state in the North-East Region of Peninsular Malaysia. A total of 132 Type 2 diabetic patients on insulin therapy were involved, with 68 participants in each control and intervention group. The health education intervention was based on the validated Diabetes Community Sharp Disposal Education Module. The knowledge and practices were measured using a validated questionnaire at baseline, one month, and three months after the intervention. Results: There was a significant increment in the mean knowledge score for intervention group; from baseline to one month follow up and from baseline to three months follow up [Greenhouse-Geisser; F(1.5, 199.7) = 62.38, p < 0.001; effect size (η2) = 0.318]. Intervention group had significantly higher mean knowledge score as compared to control group; at one month and three months follow up [F(1, 134) = 17.38, p < 0.001; effect size (η2) = 0.115]. There was a statistically significant increment in the proportion of participants in the intervention group who practiced the proper community sharp disposal method over time, X2(2) = 52.061, p < 0.001. Conclusions: The Diabetes Community Sharp Disposal Education Module was an effective health education tool to improve knowledge and encourage Malaysian diabetic patients to engage with proper sharp disposal practices.
    MeSH terms: Aged; Community Health Services; Diabetes Mellitus; Female; Hazardous Waste*; Health Education/statistics & numerical data*; Humans; Insulin/administration & dosage*; Health Knowledge, Attitudes, Practice; Malaysia; Male; Middle Aged; Primary Health Care; Surveys and Questionnaires; Medical Waste Disposal*
  7. Faiz ZM, Mardhiyyah MP, Mohamad A, Hidir A, Nurul-Hidayah A, Wong L, et al.
    Anim. Reprod. Sci., 2019 Oct;209:106143.
    PMID: 31514941 DOI: 10.1016/j.anireprosci.2019.106143
    Understanding Macrobrachium rosenbergii ovarian maturation control at the genome level is an important aspect for increasing larvae production. In this study, an ovarian maturation related gene, M. rosenbergii vWD domain and three Kazal-type domains of a gene (MrvWD-Kazal) have been studied. The MrvWD-Kazal gene was isolated using a rapid amplification of cDNA end (RACE) method and the relative abundances of MrvWD-Kazal mRNA in the ovary, hepatopancreas, stomach, intestine and gill were determined by using the quantitative PCR technique. The MrvWD-Kazal gene is composed of 2194 bp with an open reading frame (ORF) of 1998 bp encoding 665 amino acids and has great similarity to the M. nipponense vWD-Kazal gene (91%). The qPCR analyses indicated the relative abundance of MrvWD-Kazal mRNA transcript varied among different stages of ovarian function (P < 0.05), but there were no differences abundance in hepatopancreas, stomach, intestine and gill (P> 0.05). In the ovary, relative abundance of MrvWD-Kazal mRNA transcript gradually increased with ovarian maturation from Stages 1 (Spent; 1.00-fold), to 2 (Proliferative; 3.47-fold) to 3 (Premature; 6.18-fold) and decreased at Stage 4 (Mature; 1.31-fold). Differential relative abundances of MrvWD-Kazal mRNA transcript in the ovary indicate the MrvWD-Kazal protein may have an important function in ovarian maturation of M. rosenbergii. The results of this study also indicate the MrvWD-Kazal is not involved in regulation of the reproductive related function of the hepatopancreas, digestive system (stomach and intestine) and respiratory system (gill).
    MeSH terms: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Female; Fresh Water; Ovary/embryology; Ovary/growth & development; Ovary/metabolism*; RNA, Messenger/genetics; Sex Differentiation/genetics*; Sexual Maturation/genetics; von Willebrand Factor/genetics; von Willebrand Factor/chemistry*; Gene Expression Regulation, Developmental; Palaemonidae/embryology; Palaemonidae/genetics*; Palaemonidae/growth & development; Hepatopancreas/embryology; Hepatopancreas/growth & development; Hepatopancreas/metabolism; Protein Domains/genetics
  8. Bueno H, Rossello X, Pocock SJ, Van de Werf F, Chin CT, Danchin N, et al.
    J Am Coll Cardiol, 2019 09 17;74(11):1454-1461.
    PMID: 31514947 DOI: 10.1016/j.jacc.2019.06.068
    BACKGROUND: The relationship between in-hospital coronary revascularization rate (CRR) and post-discharge mortality rates in survivors of non-ST-segment elevation acute coronary syndrome (NSTE-ACS) at a system level is unclear.

    OBJECTIVES: The purpose of this study was to evaluate CRR and 2-year post-discharge mortality rate (2YMR) in NSTE-ACS.

    METHODS: CRR and 2YMR were analyzed by hospital rate of CRR (in deciles), by country, and by world region in 11,931 patients with NSTE-ACS who survived to discharge and were enrolled in the EPICOR (long-tErm follow uP of antithrombotic management patterns In acute CORonary syndrome patients) and EPICOR Asia: twin multinational, observational, prospective cohort studies.

    RESULTS: Significant differences in patient baseline characteristics, medical therapies, CRR, and 2YMR were found. Mean CRR ranged from 0.0% to 96.8% in the first and tenth decile, respectively (p 

    MeSH terms: Aged; Female; Hospitalization*; Humans; Male; Middle Aged; Patient Discharge; Prospective Studies; Time Factors; Survival Rate; Risk Assessment; Acute Coronary Syndrome/mortality*; Acute Coronary Syndrome/surgery*; Percutaneous Coronary Intervention/statistics & numerical data*
  9. Selvamani M, Bindiya PK, Bhojaraju N, Bastian TS, Suhana HS, Mathew M
    J Oral Maxillofac Pathol, 2019 9 14;23(2):295-299.
    PMID: 31516240 DOI: 10.4103/jomfp.JOMFP_277_18
    Aim: The aim of this study was to analyze and identify differences in the palatal rugae patterns and genderwise distributions in three different populations (Kerala, Mahe and Puducherry).

    Materials and Methods: Ninety maxillary study models (30 from each group including males and females) were examined in the age group ranging from 18 to 25 years. The palatal rugae pattern was analyzed for shape.

    Results: After analyzing the rugae patterns among the groups, the most common pattern was the wavy pattern (53.57%) followed by curved (18.22%) and straight (13.66%). The least was circular (1.3%). When compared between sex, the most common pattern was found to be wavy (male - 54.3% and female - 53.09%), while the curved pattern was more common among the females (21.09%) than males (13.97%). The straight pattern was more common among the males (18.8%) than females (10.18%). The least common pattern was found to be the circular in both sexes which accounted for around 1%.

    Conclusion: This study shows no two palates are identical in terms of their rugae pattern. Palatal rugae possess unique characteristics as they are absolutely individualistic. Study also confirms that the "wavy" type of palatal rugae pattern was the most predominant among these three populations.

    MeSH terms: Female; Male; Palate; Sexual Behavior; Heterosexuality
  10. Rajan ST, Narasimhan M, Rao KB, Jacob TE
    J Oral Maxillofac Pathol, 2019 9 14;23(2):303.
    PMID: 31516245 DOI: 10.4103/jomfp.JOMFP_297_18
    Background: Xylene is one of the most commonly used solvents in industrial and medical technologies. Several health hazards of xylene have been documented in literature. Workers in certain forces appear to have the greatest potential for exposure to high concentrations of xylene - histopathology technicians and painters are two such groups. This study was undertaken with the aim to determine the level of xylene exposure and the various systemic health effects among these groups.

    Methodology: The study was performed by analyzing the urine samples of the participants for methylhippuric acid, the established biomarker of xylene with the aid of high-performance liquid chromatography.

    Results and Conclusion: The work hours per week of the occupationally exposed participants were statistically analyzed with that of the excretory values of the metabolites of xylene, and the P value was found to be highly significant. Various side effects of xylene including respiratory, dermatological, neurological and gastrointestinal symptoms were observed among the study groups.

    MeSH terms: Chromatography, High Pressure Liquid; Industry; Mortuary Practice; Solvents; Xylenes; Biomarkers; Occupational Exposure
  11. Nasreen S, Rafique U, Ehrman S, Ashraf MA
    Saudi J Biol Sci, 2019 Sep;26(6):1161-1170.
    PMID: 31516345 DOI: 10.1016/j.sjbs.2015.08.014
    Water pollution due to organic compounds is of great concern and efforts are being made to develop efficient adsorbents for remediation of toxic pollutants. The development of new functionalized materials with increased performance is growing to meet the regulatory standards in response to public concerns for environment. In this study, an attempt has been made to investigate the influence of synthesis parameters like the reaction temperature, the surfactant-to-silica ratio and reaction time on the structural and textural properties of novel ordered mesoporous silica hybrids. In order to understand the effect of different synthesis parameters, all the prepared materials were systematically characterized by various analytical, spectroscopic and imaging techniques such as XRD, BET, TG etc. It was deduced from these studies that the synthesis temperature influence greatly the structural order whereas both the P104/Na2SiO3 molar ratio and reaction time found to influence textural properties significantly. However, under optimized experimental condition, we could achieve the functionalized silica hybrids that offers successful incorporation of -Amino, -Glucidoxy, -Methacrylate, -Vinyl and -Phenyl moieties indicated by FTIR peaks at 793 cm-1, 2870 cm-1, 796 cm-1, 1630 cm-1 and 954 cm-1. XRD studies reveal orthorhombic and tetragonal symmetry for the hybrids and these materials were found to be thermally stable due to incorporation of organic moiety in silica matrix. Functionalized silica hybrids then applied as adsorbents demonstrated efficient and comparable removal of 4-aminophenol and p-nitrophenol in 20 min facilitated through organic moiety. Detailed modeling of the sorption using equilibrium and kinetic isotherms has been carried out to get an insight into the transport process. The adsorption isotherms of phenol derivatives are well-fitted with the Langmuir, Freundlich and Temkin Isotherms and the adsorption kinetics follows the pseudo second order model. The modeling confirms that the uptake is a chemisorption process.
  12. Khoo ACH, Chen SL
    World J Nucl Med, 2019 9 14;18(3):301-303.
    PMID: 31516376 DOI: 10.4103/wjnm.WJNM_43_18
    Follicular thyroid cancers are known to spread hematogenously to the bones and lungs and rarely presenting with massive angioinvasion. We report a case of a middle-aged female who had undergone total thyroidectomy for minimally invasive follicular thyroid cancer with angioinvasion in 2014. She was noted to have a large tumor thrombus extending from the superior vena cava to the right atrium on whole body scan post-Iodine-131 (131I) remnant ablation therapy. We discuss the various imaging modalities, treatment options, and difficulties in managing such massive angioinvasion in patients with well-differentiated thyroid cancers.
    MeSH terms: Female; Heart Atria; Humans; Iodine Radioisotopes; Middle Aged; Thrombosis; Thyroid Neoplasms; Thyroidectomy; Vena Cava, Superior; Adenocarcinoma, Follicular; Whole Body Imaging
  13. Manvati S, Mangalhara KC, Kalaiarasan P, Chopra R, Agarwal G, Kumar R, et al.
    Cancer Cell Int, 2019;19:230.
    PMID: 31516387 DOI: 10.1186/s12935-019-0933-8
    Background: Despite several reports describing the dual role of miR-145 as an oncogene and a tumor suppressor in cancer, not much has been resolved and understood.

    Method: In this study, the potential targets of miR-145 were identified bio-informatically using different target prediction tools. The identified target genes were validated in vitro by dual luciferase assay. Wound healing and soft agar colony assay assessed cell proliferation and migration. miR-145 expression level was measured quantitatively by RT-PCR at different stages of breast tumor. Western blot was used to verify the role of miR-145 in EMT transition using key marker proteins.

    Result: Wound healing and soft agar colony assays, using miR-145 over-expressing stably transfected MCF7 cells, unraveled its role as a pro-proliferation candidate in cancerous cells. The association between miR-145 over-expression and differential methylation patterns in representative target genes (DR5, BCL2, TP53, RNF8, TIP60, CHK2, and DCR2) supported the inference drawn. These in vitro observations were validated in a representative set of nodal positive tumors of stage 3 and 4 depicting higher miR-145 expression as compared to early stages. Further, the role of miR-145 in epithelial-mesenchymal (EMT) transition found support through the observation of two key markers, Vimentin and ALDL, where a positive correlation with Vimentin protein and a negative correlation with ALDL mRNA expression were observed.

    Conclusion: Our results demonstrate miR-145 as a pro-cancerous candidate, evident from the phenotypes of aggressive cellular proliferation, epithelial to mesenchymal transition, hypermethylation of CpG sites in DDR and apoptotic genes and upregulation of miR-145 in later stages of tumor tissues.

    MeSH terms: Agar; Breast Neoplasms; Humans; Luciferases; Methylation; Oncogenes; Phenotype; RNA, Messenger; Vimentin; Wound Healing; Blotting, Western; Up-Regulation; Genes, Tumor Suppressor; Reverse Transcriptase Polymerase Chain Reaction; MicroRNAs; Cell Proliferation; Epithelial-Mesenchymal Transition; MCF-7 Cells
  14. Lim KH, Lim HL, Teh CH, Ghazali SM, Kee CC, Heng PP, et al.
    Tob Induc Dis, 2019;17:51.
    PMID: 31516494 DOI: 10.18332/tid/100692
    INTRODUCTION: Studies have shown that the implementation of smoke-free policies at workplaces have shifted the social norms towards secondhand smoke (SHS) exposure at home. This study aimed to investigate whether working in a smoke-free workplace is associated with living in a smoke-free home (SFH).

    METHODS: The data were derived from the Malaysian Global Adult Tobacco Survey (GATS-M), collected in 2011-2012, involving 4250 respondents. Data analyses involved 1343 respondents reported to be in the working population.

    RESULTS: More than half of the respondents (58.5%) were reportedly working in smoke-free workplaces. Almost a quarter (24.8%) of those who worked in smoke-free workplaces stayed in smoke-free homes, which was more than two times higher than their counterparts who worked at non-smoke-free workplaces (24.8% vs 12.0%, p<0.001). Multivariable analyses further substantiated this finding (AOR=2.01, 95% CI: 1.11-3.61, reference group = worked at non-smoke-free workplaces).

    CONCLUSIONS: This study found an association between living in smoke-free homes and working at smoke-free workplaces, which could suggest a positive impact of implementing smoke-free workplaces.

  15. Zainal NS, Lee BKB, Wong ZW, Chin IS, Yee PS, Gan CP, et al.
    Cancer Biol Med, 2019 May;16(2):264-275.
    PMID: 31516747 DOI: 10.20892/j.issn.2095-3941.2018.0257
    Objective: Lack of effective therapies remains a problem in the treatment of oral squamous cell carcinoma (OSCC), especially in patients with advanced tumors. OSCC development is driven by multiple aberrancies within the cell cycle pathway, including amplification of cyclin D1 and loss of p16. Hence, cell cycle inhibitors of the CDK4/6-cyclin D axis are appealing targets for OSCC treatment. Here, we determined the potency of palbociclib and identified genetic features that are associated with the response of palbociclib in OSCC.

    Methods: The effect of palbociclib was evaluated in a panel of well-characterized OSCC cell lines by cell proliferation assays and further confirmed by in vivo evaluation in xenograft models. PIK3CA-mutant isogenic cell lines were used to investigate the effect of PIK3CA mutation towards palbociclib response.

    Results: We demonstrated that 80% of OSCC cell lines are sensitive to palbociclib at sub-micromolar concentrations. Consistently, palbociclib was effective in controlling tumor growth in mice. We identified that palbociclib-resistant cells harbored mutations in PIK3CA. Using isogenic cell lines, we showed that PIK3CA mutant cells are less responsive to palbociclib as compared to wild-type cells with concurrent upregulation of CDK2 and cyclin E1 protein levels. We further demonstrated that the combination of a PI3K/mTOR inhibitor (PF-04691502) and palbociclib completely controlled tumor growth in mice.

    Conclusions: This study demonstrated the potency of palbociclib in OSCC models and provides a rationale for the inclusion of PIK3CA testing in the clinical evaluation of CDK4/6 inhibitors and suggests combination approaches for further clinical studies.

    MeSH terms: Animals; Cell Cycle; Cell Line; Humans; Mouth Neoplasms; Mutation; Piperazines; Pyridines; Oncogene Proteins; Up-Regulation; Phosphatidylinositol 3-Kinases; Cyclin E; Cyclin D1; Cell Proliferation; Mice; Cyclin D; Heterografts
  16. Athar Abbasi M, Yu SM, Aziz-Ur-Rehman, Siddiqui SZ, Kim SJ, Raza H, et al.
    Toxicol Rep, 2019;6:897-903.
    PMID: 31516842 DOI: 10.1016/j.toxrep.2019.08.016
    In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule (7) was synthesized and its structural confirmation was carried out by IR, 1H-NMR, 13C-NMR and CHN analysis data. This compound 7 was subjected to biological study with B16F10 mouse melanoma cells. The anti-proliferative results showed that 7 showed no significant toxicity at concentrations ranging of 0-44 μM. The treatment of B16F10 cells with 7 at aforementioned concentration range indicated that migration of cells was significantly lower than that of the control cells in a dose dependent manner. The possible migration inhibitory effect of these melanoma cells was further evaluated through gelatinolytic activity of MMP-2 and MMP-9 secreted from B16F10 cells. It was inferred from our results that 7 was not affecting the expression and activity of these enzymes. Some other zinc-dependent matrix metalloproteinases (MMPs) were involved in the inhibitory progression. Taken together, compound 7 inhibited migrations of B16F10 mouse melanoma cells. Therefore, it may deserve consideration as a potential agent for the treatment of cancer.
  17. Ismail R
    Data Brief, 2019 Aug;25:104166.
    PMID: 31516921 DOI: 10.1016/j.dib.2019.104166
    Generally, a base isolator is made up of alternate layers of steel and rubber. The idea of adopting magnetoreological elastomers (MREs) in base isolator systems was introduced in the past few years in order to improve the efficiency of base isolator systems. The article provides information on the mechanical corresponding to different carbon black loading loadings of 20 parts per hundred rubber (pphr), 40 pphr and 60 pphr in natural rubber compound. The mechanical dataset described the data from tensile, hardness and rebound test.
    MeSH terms: Hardness; Rubber; Steel; Elastomers; Soot
  18. Omar A, Jalil JA, Shakrin NM, Ngu LH, Yunus ZM
    Data Brief, 2019 Aug;25:104377.
    PMID: 31516928 DOI: 10.1016/j.dib.2019.104377
    This article contains information related to a recent study "Selective screening for detection of mucopolysaccharidoses (MPS) in Malaysia; A Two-year Study" Affandi et al., 2019. Any patient registered under government healthcare facilities in Malaysia and fit at least two inclusion criteria were included in this selective screening. Urine and blood from these high risk patients were obtained and analysed for glycosaminoglycans (GAGs) level before characterization using high resolution electrophoresis (HRE). Thereafter, enzyme assay for different types of MPS based on result of HRE were determined using specific substrate. Demographic data as well as laboratory findings were tabulated and analysed. The data of this study demonstrate between clinical presentation and laboratory findings among high risk patients of MPS and can be employed to improve diagnosis of MPS.
    MeSH terms: Demography; Electrophoresis; Glycosaminoglycans; Government; Humans; Malaysia; Mucopolysaccharidoses; Enzyme Assays
  19. Perumal L
    Heliyon, 2019 Aug;5(8):e02319.
    PMID: 31517093 DOI: 10.1016/j.heliyon.2019.e02319
    New techniques are presented for Delaunay triangular mesh generation and element optimisation. Sample points for triangulation are generated through mapping (a new approach). These sample points are later triangulated by the conventional Delaunay method. Resulting triangular elements are optimised by addition, removal and relocation of mapped sample points (element nodes). The proposed techniques (generation of sample points through mapping for Delaunay triangulation and mesh optimisation) are demonstrated by using Mathematica software. Simulation results show that the proposed techniques are able to form meshes that consist of triangular elements with aspect ratio of less than 2 and minimum skewness of more than 45°.
    MeSH terms: Software; Medical Subject Headings
  20. Asghar A, Bello MM, Raman AAA, Daud WMAW, Ramalingam A, Zain SBM
    Heliyon, 2019 Sep;5(9):e02396.
    PMID: 31517121 DOI: 10.1016/j.heliyon.2019.e02396
    In this work, quantum chemical analysis was used to predict the degradation potential of a recalcitrant dye, Acid blue 113, by hydrogen peroxide, ozone, hydroxyl radical and sulfate radical. Geometry optimization and frequency calculations were performed at 'Hartree Fock', 'Becke, 3-parameter, Lee-Yang-Parr' and 'Modified Perdew-Wang exchange combined with PW91 correlation' levels of study using 6-31G* and 6-31G** basis sets. The Fourier Transform-Raman spectra of Acid blue 113 were recorded and a complete analysis on vibrational assignment and fundamental modes of model compound was performed. Natural bond orbital analysis revealed that Acid blue 113 has a highly stable structure due to strong intermolecular and intra-molecular interactions. Mulliken charge distribution and molecular electrostatic potential map of the dye also showed a strong influence of functional groups on the neighboring atoms. Subsequently, the reactivity of the dye towards the oxidants was compared based on the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) energy values. The results showed that Acid blue 113 with a HOMO value -5.227 eV exhibits a nucleophilic characteristic, with a high propensity to be degraded by ozone and hydroxyl radical due to their lower HOMO-LUMO energy gaps of 4.99 and 4.22 eV respectively. On the other hand, sulfate radical and hydrogen peroxide exhibit higher HOMO-LUMO energy gaps of 7.92 eV and 8.10 eV respectively, indicating their lower reactivity towards Acid blue 113. We conclude that oxidation processes based on hydroxyl radical and ozone would offer a more viable option for the degradation of Acid blue 113. This study shows that quantum chemical analysis can assist in selecting appropriate advanced oxidation processes for the treatment of textile effluent.
    MeSH terms: Azo Compounds; Fourier Analysis; Hydrogen Peroxide; Ozone; Sulfates; Textiles; Vibration; Oxidants; Hydroxyl Radical; Static Electricity
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