Browse publications by year: 2021

  1. Liew A, Lee CC, Lan BL, Tan M
    Comput Biol Med, 2021 09;136:104690.
    PMID: 34352452 DOI: 10.1016/j.compbiomed.2021.104690
    Convolutional neural networks (CNNs) have been used quite successfully for semantic segmentation of brain tumors. However, current CNNs and attention mechanisms are stochastic in nature and neglect the morphological indicators used by radiologists to manually annotate regions of interest. In this paper, we introduce a channel and spatial wise asymmetric attention (CASPIAN) by leveraging the inherent structure of tumors to detect regions of saliency. To demonstrate the efficacy of our proposed layer, we integrate this into a well-established convolutional neural network (CNN) architecture to achieve higher Dice scores, with less GPU resources. Also, we investigate the inclusion of auxiliary multiscale and multiplanar attention branches to increase the spatial context crucial in semantic segmentation tasks. The resulting architecture is the new CASPIANET++, which achieves Dice Scores of 91.19%, 87.6% and 81.03% for whole tumor, tumor core and enhancing tumor respectively. Furthermore, driven by the scarcity of brain tumor data, we investigate the Noisy Student method for segmentation tasks. Our new Noisy Student Curriculum Learning paradigm, which infuses noise incrementally to increase the complexity of the training images exposed to the network, further boosts the enhancing tumor region to 81.53%. Additional validation performed on the BraTS2020 data shows that the Noisy Student Curriculum Learning method works well without any additional training or finetuning.
    MeSH terms: Curriculum; Humans; Image Processing, Computer-Assisted*; Students; Neural Networks (Computer)
  2. Cheah FC, Tan TL
    World Rev Nutr Diet, 2021;122:340-356.
    PMID: 34352769 DOI: 10.1159/000514761
    MeSH terms: Developing Countries*; Humans; Infant; Infant Nutritional Physiological Phenomena; Infant, Newborn; Infant, Premature*; Nutritional Status; Nutritional Support
  3. Koletzko B, Wieczorek S, Cheah FC, Domellöf M, van Goudoever JB, Poindexter BB, et al.
    World Rev Nutr Diet, 2021;122:191-197.
    PMID: 34352778 DOI: 10.1159/000514772
    MeSH terms: Energy Intake; Eating; Humans; Infant; Infant Nutritional Physiological Phenomena*; Infant, Newborn; Infant, Premature*; Nutritional Requirements
  4. Bilal S, Abdulla AM, Andiesta NS, Babar MG, Pau A
    Health Qual Life Outcomes, 2021 Aug 03;19(1):192.
    PMID: 34344379 DOI: 10.1186/s12955-021-01828-3
    BACKGROUND: The aim of this cross-sectional study was to evaluate the effect of family functioning on oral health related quality of life (OHRQoL) and dental caries status among 4- to 6-year-old Chinese pre-school children in Malaysia.

    METHODOLOGY: This study was approved by the institutional Joint Research and Ethics Committee, International Medical University, Malaysia (number 373/2016); consisted of 180 eligible pre-school children from a private school. Study tools included demographic, clinical oral health data form, the Early Childhood Oral Health Impact Scale (ECOHIS) and family functioning-12-item general functioning subscale. Written consent was sought prior to data collection. Data were analysed by SPSS v.22.0; descriptive statistics for socio-demographic details, clinical information, HRQoL and FAD scores. The parametric tests included independent sample t test and ANOVA to evaluate the associations between the dependent variable. Binary logistic regression models were applied to assess the impacts on OHRQoL (P value 

    MeSH terms: Child; Child, Preschool; Cross-Sectional Studies; Dental Caries/epidemiology; Dental Caries/psychology*; Family/psychology*; Family Relations*; Female; Humans; Male; Oral Health; Quality of Life*; Surveys and Questionnaires
  5. Jun Xin L, Ahmad Hathim AA, Jing Yi N, Reiko A, Noor Akmal Shareela I
    BMC Med Educ, 2021 Aug 04;21(1):418.
    PMID: 34344388 DOI: 10.1186/s12909-021-02855-w
    BACKGROUND: Medical education has undergone a transformation from conventional to digital learning, enabling learning without any time and place restrictions. Nevertheless, the actual trends of usage and its impact on learning motivation among medical students between developed and developing nations are yet to be investigated. Hence, this study compares the effect of digital learning on learning motivation among Malaysian and Japanese medical students in Universiti Kebangsaan Malaysia (UKM) and Shiga University of Medical Science (SUMS) respectively.

    METHODS: A modified Students Motivation towards Science Learning (SMTSL) was used to assess the digital learning usage and learning motivation among 150 UKM and 147 SUMS medical students throughout Year 1 to 5.

    RESULTS: The frequency of digital learning usage and learning motivation among UKM medical students was significantly higher as compared to SUMS (p 

    MeSH terms: Education, Medical*; Humans; Japan; Learning; Malaysia; Motivation; Students, Medical*
  6. Lee EL, Haseeb ASMA, Basirun WJ, Wong YH, Sabri MFM, Low BY
    Sci Rep, 2021 Aug 03;11(1):15768.
    PMID: 34344974 DOI: 10.1038/s41598-021-95276-0
    The miniaturization of electronic devices and the consequent decrease in the distance between conductive lines have increased the risk of short circuit failure due to electrochemical migration (ECM). The presence of ionic contaminants affects the ECM process. This work systematically investigates the ECM of tin (Sn) in the presence of bromide ions (Br-) in the range of 10-6 M to 1.0 M. Water drop test (WDT) was conducted in the two-probe semiconductor characterization system under an optical microscope as an in-situ observation. Polarization test was carried out to study the correlation between the corrosion properties of Sn and its ECM behaviour. The products of ECM were characterized by scanning electron microscope coupled with an energy dispersive X-rays spectrometer (SEM/EDX) and X-ray photoelectron spectrometer (XPS). The results confirm that the rate of anodic dissolution of Sn monotonously increases with the Br- concentration. However, the probability of ECM failure follows a normal distribution initially, but later increases with the Br- concentration. The main products of the ECM reactions are identified as Sn dendrites and tin hydroxide precipitates. The mechanisms of the ECM process of Sn in the presence of Br- are also suggested.
  7. Wan Chek WAF, Teh YG, Eddy Suryono DN, Ng CY, Ahmed S
    Radiol Case Rep, 2021 Sep;16(9):2706-2709.
    PMID: 34345334 DOI: 10.1016/j.radcr.2021.06.061
    Hypothalamic hamartomas (HHs) are non-neoplastic malformations that occur in the region of the hypothalamus. HH is the leading cause of gelastic seizures in children and adolescents, where laughing is characteristically manifested. However, these patients can also experience different forms of complex or generalized tonic-clonic seizures that can obscure the diagnosis of HHs. We present a case of a 10 year-old boy that experienced several seizure types, but was subsequently diagnosed with HH after MR imaging was performed. This case highlights the complementary role of MR imaging in ascertaining seizure etiololgy when the clinical history and EEG findings are non-specific. The importance of early diagnosis with MR imaging is further underscored by the fact that patients diagnosed with HH usually develop drug resistance towards antiepileptic drugs, mandating neurosurgical assessment and intervention.
  8. Mardina V, Ilyas S, Halimatussakdiah H, Harmawan T, Tanjung M, Yusof F
    J Adv Pharm Technol Res, 2021 07 16;12(3):222-226.
    PMID: 34345598 DOI: 10.4103/japtr.JAPTR_131_21
    This study aims to investigate the potential of bioactive secondary metabolites contained in Sphagneticola trilobata (L.) J.F Pruski leaves as novel plant-derived anticancer agent. Qualitative bioactive compound contents in the methanolic extract of S. trilobata leaves were screened using phytochemical method. Antioxidant evaluation was carried out using 2,2-diphenyl-1-picrylhydrazyl assay; antibacterial - using well diffusion method on Escherichia coli and Salmonella typhi; and cytotoxicity - using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay on MCF-7 cell line and Vero Cell. It was found that the methanolic extract exhibited antioxidant activity with an IC50 value of 124.34 μg/mL. The inhibition zone values against E. coli and S. thypi (at extract concentration of 100 mg/mL) were 34.33 and 36 mm, respectively. In vitro MTT assay showed that our extract successfully reached 96% mortality with LC50 = 189.287 μg/mL, where the selective index of 2.5 suggest its selectivity against MCF-7 breast cancer cell line. In conclusion, the data of biological activities suggest the potential development of methanolic extract from S. trilobata leaves as a phytomedicine for breast cancer treatment.
  9. Aminah S, Huda F, Gamayani U, Pusparini I, Mochyadin MFA, Sribudiani Y, et al.
    Heliyon, 2021 Jul;7(7):e07519.
    PMID: 34345727 DOI: 10.1016/j.heliyon.2021.e07519
    Spinocerebellar ataxia (SCA) is an autosomal dominant hereditary disease with progressive course, and no causal therapy. Diagnostics are still challenging, due to facility and protocols, and so as in Indonesia. As a national referral center, Dr. Hasan Sadikin Central General Hospital has received a lot of patients from all over Indonesia, particularly from Western Java. Study related to SCA (including clinical and genetic profile) is still limited in Indonesia. We identified index patients from three families with ataxia, hence intend to determine their clinical and genetic pattern. The hereditary pattern is autosomal dominant. Scale for the assessment and rating of ataxia (SARA) shows mild and moderate ataxia. Inventory of non-ataxia signs (INAS) scores of the patients were 3, 5 and 6. Montreal cognitive assessment-Indonesian version (MOCA-INA) shows only one patient has mild cognitive impairment, despite young age. Barthel index shows 1 subject has moderate dependency. Mutation in Ataxin3 polyQ repeats shows pathologically long CAG repeats, 72,10; 72,10; and 72,23 respectively in mutant and wild type allele. We diagnosed the index patients with spinocerebellar ataxia type 3. This study is the first case series study in Indonesia. The hereditary pattern is clearly shown as an autosomal dominant ataxia. The clinical and genetic profile was varied, and the symptom is progressive and deteriorates overtime, including wide based gait, speech problem, motor and sensor complaint, and cognitive decline complaint. Despite the same polyQ stretch length, the onset and clinical characteristics of patients are diverse.
  10. Alzubaidi M, Zubaydi HD, Bin-Salem AA, Abd-Alrazaq AA, Ahmed A, Househ M
    PMID: 34345877 DOI: 10.1016/j.cmpbup.2021.100025
    BACKGROUND: Since the onset of the COVID-19 pandemic, the world witnessed disruption on an unprecedented scale affecting our daily lives including but not limited to healthcare, business, education, and transportation. Deep Learning (DL) is a branch of Artificial intelligence (AI) applications, the recent growth of DL includes features that could be helpful in fighting the COVID-19 pandemic. Utilizing such features could support public health efforts.

    OBJECTIVE: Investigate the literature available in the use of DL technology to support dealing with the COVID-19 crisis. We summarize the literature that uses DL features to analyze datasets for the purpose of a quick COVID-19 detection.

    METHODS: This review follows PRISMA Extension for Scoping Reviews (PRISMA-ScR). We have scanned the most two commonly used databases (IEEE, ACM). Search terms were identified based on the target intervention (DL) and the target population (COVID-19). Two authors independently handled study selection and one author assigned for data extraction. A narrative approach is used to synthesize the extracted data.

    RESULTS: We retrieved 53 studies and after passing through PRISMA excluding criteria, only 17 studies are considered in this review. All studies used deep learning for detection of COVID-19 cases in early stage based on different diagnostic modalities. Convolutional Neural Network (CNN) and Transfer Learning (TL) were the most commonly used techniques.

    CONCLUSION: The included studies showed that DL techniques has significant impact on early detection of COVID-19 with high accuracy rate. However, most of the proposed methods are still in development and not tested in a clinical setting. Further investigation and collaboration are required from the research community and healthcare professionals in order to develop and standardize guidelines for use of DL in the healthcare domain.

  11. Mohd Maidin NN, Buyong MR, A Rahim R, Mohamed MA
    Electrophoresis, 2021 10;42(20):2033-2059.
    PMID: 34346062 DOI: 10.1002/elps.202100043
    Dielectrophoresis (DEP) is a technique to manipulate trajectories of polarisable particles in nonuniform electric fields by utilizing unique dielectric properties. The manipulation of a cell using DEP has been demonstrated in various modes, thereby indicating potential applications in the biomedical field. In this review, recent DEP applications in the biomedical field are discussed. This review is intended to highlight research work that shows significant approach related to DEP application in biomedical field reported between 2016 and 2020. First, single-shell model and multiple-shell model of cells are introduced. Current device structures and recently introduced electrode patterns for DEP applications are discussed. Second, the biomedical uses of DEP in liquid biopsies, stem cell-based therapies, and diagnosis of infectious diseases due to bacteria and viruses are presented. Finally, the challenges in DEP research are discussed, and the reported solutions are explained. DEP's potential research directions are mentioned.
  12. Stevanovic D, Costanzo F, Fucà E, Valeri G, Vicari S, Robins DL, et al.
    Autism Res, 2021 12;14(12):2544-2554.
    PMID: 34346193 DOI: 10.1002/aur.2586
    The Childhood Autism Rating Scale (CARS) is a simple and inexpensive tool for Autism spectrum disorder (ASD) assessments, with evidenced psychometric data from different countries. However, it is still unclear whether ASD symptoms are measured the same way across different societies and world regions with this tool, since data on its cross-cultural validity are lacking. This study evaluated the cross-cultural measurement invariance of the CARS among children with ASD from six countries, for whom data were aggregated from previous studies in India (n = 101), Jamaica (n = 139), Mexico (n = 72), Spain (n = 99), Turkey (n = 150), and the United States of America (n = 186). We analyzed the approximate measurement invariance based on Bayesian structural equation modeling. The model did not fit the data and its measurement invariance did not hold, with all items found non-invariant across the countries. Items related to social communication and interaction (i.e., relating to people, imitation, emotional response, and verbal and nonverbal communication) displayed lower levels of cross-country non-invariance compared to items about stereotyped behaviors/sensory sensitivity (i.e., body and object use, adaptation to change, or taste, smell, and touch response). This study found that the CARS may not provide cross-culturally valid ASD assessments. Thus, cross-cultural comparisons with the CARS should consider first which items operate differently across samples of interest, since its cross-cultural measurement non-invariance could be a source of cross-cultural variability in ASD presentations. Additional studies are needed before drawing valid recommendations in relation to the cultural sensitivity of particular items.
  13. Rasool M, Malik A, Abdul Basit Ashraf M, Mubbin R, Ayyaz U, Waquar S, et al.
    Bioengineered, 2021 12;12(1):4593-4604.
    PMID: 34346287 DOI: 10.1080/21655979.2021.1955528
    The Vaccinium genus comprises more than 126 genera of perennial flowering plants that are commonly adapted to poor and acidic soils or epiphytic environments. Their molecular and genomic characterization is a result of the recent advent in next-generation sequencing technology. In the current research, extracts were prepared in different media, such as petroleum ether, methanol and ethanol. An extract of Vaccinium macrocarpon (cranberry) was used at a dose of 200-400 mg/kg by weight (B.wt). Levels of oxidative stress markers, i.e., superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), advanced oxidation protein products (AOPPs) and malondialdehyde (MDA), were measured. A histopathological study of six vital organs in rats was also conducted. The results indicated that the antioxidant levels were lower in the group given only ethylene oxide (EtO) but higher in the groups receiving cranberry extract as a treatment. Major improvements were also observed in stress markers such as advanced oxidation protein products (AOPPs) and MDA following cranberry treatment. Histopathological changes induced by EtO were observed in the heart, kidney, liver, lung, stomach and testis and were reversed following cranberry treatment. The major toxic effects of EtO were oxidative stress and organ degeneration, as observed from various stress markers and histopathological changes. Our study showed that this extract contains strong antioxidant properties, which may contribute to the amelioration of the observed toxic effects.
    MeSH terms: Animals; Ethylene Oxide/toxicity*; Plant Extracts/pharmacology; Plant Extracts/chemistry; Oxidative Stress/drug effects*; Vaccinium macrocarpon*; Rats
  14. Nguyen DTC, Le HTN, Nguyen TT, Nguyen TTT, Liew RK, Bach LG, et al.
    Sci Total Environ, 2021 Nov 25;797:149195.
    PMID: 34346381 DOI: 10.1016/j.scitotenv.2021.149195
    Asteraceae presents one of the most globally prevalent, cultivated, and fundamental plant families. However, a large amount of agricultural wastes has been yearly released from Asteraceae crops, causing adverse impacts on the environment. The objective of this work is to have insights into their biomass potentials and technical possibility of conversion into biochars. Physicochemical properties are systematically articulated to orientate environmental application, soil amendment, and other utilizations. Utilizations of Asteraceae biochars in wastewater treatment can be categorized by heavy metal ions, organic dyes, antibiotics, persistent organic pollutants (POPs), and explosive compounds. Some efforts were made to analyze the production cost, as well as the challenges and prospects of Asteraceae-based biochars.
    MeSH terms: Charcoal; Humans; Soil; Asteraceae*
  15. Zhang D, Hussain HI
    J Environ Manage, 2021 Nov 01;297:113360.
    PMID: 34346402 DOI: 10.1016/j.jenvman.2021.113360
    The triple components of energy consumption, carbon dioxide emissions, and economic expansion are important to achieving sustained economic activity and sound ecological advancement. This study aims to estimate the impact of wide-ranging parameters on environmental circumstances in South Asian countries. This analysis required two approaches: 1)quantile autoregressive distributed lag (QARDL) as an econometric model, and 2) data envelopment analysis (DEA) non-parametric comparable composite index to examine concurrently South Asian nations' data for the 2000-2018 period. The underscored category of the parameters were grouped into four key indices, namely financial, fiscal, human, and energy. The DEA's mathematical composite findings reveal varied circumstances regarding environmental self-maintenance in South Asian nations. India and Pakistan are doing quite well; Afghanistan is abysmal. In addition, the QARDL approach findings reveal that energy use and fiscal indicators abate pollution. Furthermore, the correlation between fiscal decentralization and ecological attributes is strengthened by the excellent level of institutions and human capital progress. There is a unidirectional impact emanating from fiscal devolution, gross domestic product, human capital, eco-innovation, and institutional excellence on carbon dioxide pollution, although different from the other correlations obtained.
    MeSH terms: Environmental Pollution; Humans; Models, Econometric; Economic Development*; Gross Domestic Product
  16. Mat Rodi MS, Vaseenon T, Phanphaisarn A, Hayadin NM
    PMID: 34347125 DOI: 10.1007/s00402-021-04102-9
    INTRODUCTION: The minimally invasive approach for displaced intra-articular calcaneal fractures is significantly reducing postoperative wound complications. One minimally invasive method, the sinus tarsi approach (STA) has been increasingly widely used. STA is, however, challenging due to its technical demands and the risk of injury to the sural nerve (SN). The purpose of this study was to identify the SN and its branches including their anatomical relationship to the STA as well as to describe an anatomical windows technique for STA including determination of the safe angle for screw insertion into the sustentaculum tali fragment.

    METHODS: Thirty-two adult cadaveric legs were disarticulated at the knee and unpaired. STA was performed on each specimen. The anatomy and distribution of the sural nerve and its branches were identified in relation to the incision. Three surgical windows were identified and selected. Kirshner wires were inserted in pairs via each of the windows towards the center of the sustentaculum tali. The safe angle for wire insertion in relation to the SN or its branches was then measured as well as the appropriate intraoperative drilling angle.

    RESULTS: The plantar branch presented in the distal window in none of the samples, while the dorsal branches presented in 37.5% and the main SN presented in only 6.25%. In the middle window, the dorsal branch presented most often (43.75%) followed by the plantar branch (25.00%) and the SN (21.88%). In the proximal window, the SN presented in 100% of the samples, while the dorsal branch presented in none and the plantar branch presented in about 15.63% of the specimens. All three windows had their own acceptable average angle for screw insertion towards the sustentaculum tali.

    CONCLUSIONS: The distal window is the safest for surgical approach and for calcaneal surgery screw fixation in terms of avoiding sural nerve injury. In addition, that window provides a wide working angle for screw fixation.

  17. Ding Y, Saw WY, Tan LWL, Moong DKN, Nagarajan N, Teo YY, et al.
    Appl Environ Microbiol, 2021 09 28;87(20):e0048821.
    PMID: 34347523 DOI: 10.1128/AEM.00488-21
    Multidrug-resistant (MDR) Escherichia coli strains that carry extended-spectrum β-lactamases (ESBLs) or colistin resistance gene mcr-1 have been identified in the human gut at an increasing incidence worldwide. In this study, we isolated and characterized MDR Enterobacteriaceae from the gut microbiota of healthy Singaporeans and show that the detection rates for ESBL-producing and mcr-positive Enterobacteriaceae are 25.7% (28/109) and 7.3% (8/109), respectively. Whole-genome sequencing analysis of the 37 E. coli isolates assigned them into 25 sequence types and 6 different phylogroups, suggesting that the MDR E. coli gut colonizers are highly diverse. We then analyzed the genetic context of the resistance genes and found that composite transposons played important roles in the cotransfer of blaCTX-M-15/55 and qnrS1, as well as the acquisition of mcr-1. Furthermore, comparative genomic analysis showed that 12 of the 37 MDR E. coli isolates showed high similarity to ESBL-producing E. coli isolates from raw meat products in local markets. By analyzing the core genome single nucleotide polymorphisms (SNPs) shared by these isolates, we identified possible clonal transmission of an MDR E. coli clone between human and raw meat, as well as a group of highly similar IncI2 (Delta) plasmids that might be responsible for the dissemination of mcr-1 in a much wider geographic region. Together, these results suggest that antibiotic resistance may be transmitted between different environmental settings by the expansion of MDR E. coli clones, as well as by the dissemination of resistance plasmids. IMPORTANCE The human gut can harbor both antibiotic-resistant and virulent Escherichia coli which may subsequently cause infections. In this study, we found that multidrug-resistant (MDR) E. coli isolates from the gut of healthy Singaporeans carry a diverse range of antibiotic resistance mechanisms and virulence factor genes and are highly diverse. By comparing their genomes with the extended-spectrum β-lactamase (ESBL)-producing E. coli isolates from raw meat products that were sampled at a similar time from local markets, we detected an MDR E. coli clone that was possibly transmitted between humans and raw meat products. Furthermore, we also found that a group of resistance plasmids might be responsible for the dissemination of colistin resistance gene mcr-1 in Singapore, Malaysia, and Europe. Our findings call for better countermeasures to block the transmission of antibiotic resistance.
  18. Quek A, Kassim NK, Lim PC, Tan DC, Mohammad Latif MA, Ismail A, et al.
    Pharm Biol, 2021 Dec;59(1):964-973.
    PMID: 34347568 DOI: 10.1080/13880209.2021.1948065
    CONTEXT: Melicope latifolia (DC.) T. G. Hartley (Rutaceae) was reported to contain various phytochemicals including coumarins, flavonoids, and acetophenones.

    OBJECTIVE: This study investigates the antidiabetic and antioxidant effects of M. latifolia bark extracts, fractions, and isolated constituents.

    MATERIALS AND METHODS: Melicope latifolia extracts (hexane, chloroform, and methanol), fractions, and isolated constituents with varying concentrations (0.078-10 mg/mL) were subjected to in vitro α-amylase and dipeptidyl peptidase-4 (DPP-4) inhibitory assay. Molecular docking was performed to study the binding mechanism of active compounds towards α-amylase and DPP-4 enzymes. The antioxidant activity of M. latifolia fractions and compounds were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene bleaching assays.

    RESULTS: Melicope latifolia chloroform extract showed the highest antidiabetic activity (α-amylase IC50: 1464.32 μg/mL; DPP-4 IC50: 221.58 μg/mL). Fractionation of chloroform extract yielded four major fractions (CF1-CF4) whereby CF3 showed the highest antidiabetic activity (α-amylase IC50: 397.68 μg/mL; DPP-4 IC50: 37.16 μg/mL) and resulted in β-sitosterol (1), halfordin (2), methyl p-coumarate (3), and protocatechuic acid (4). Isolation of compounds 2-4 from the species and their DPP-4 inhibitory were reported for the first time. Compound 2 showed the highest α-amylase (IC50: 197.53 μM) and β-carotene (88.48%) inhibition, and formed the highest number of molecular interactions with critical amino acid residues of α-amylase. The highest DPP-4 inhibition was exhibited by compound 3 (IC50: 911.44 μM).

    DISCUSSION AND CONCLUSIONS: The in vitro and in silico analyses indicated the potential of M. latifolia as an alternative source of α-amylase and DPP-4 inhibitors. Further pharmacological studies on the compounds are recommended.

    MeSH terms: alpha-Amylases/antagonists & inhibitors*; alpha-Amylases/chemistry; Antioxidants/pharmacology; Antioxidants/chemistry; Computer Simulation; Humans; Hypoglycemic Agents/pharmacology; Hypoglycemic Agents/chemistry; Plant Extracts/isolation & purification; Plant Extracts/pharmacology*; Plant Extracts/chemistry*; Molecular Structure; Dipeptidyl Peptidase 4; Plant Bark/chemistry; Rutaceae/chemistry*; Dipeptidyl-Peptidase IV Inhibitors/chemistry*; Molecular Docking Simulation; Phytochemicals/pharmacology; Phytochemicals/chemistry; In Vitro Techniques
  19. Kazmi I, Alharbi KS, Al-Abbasi FA, Almalki WH, G SK, Yasmeen A, et al.
    Crit Rev Eukaryot Gene Expr, 2021;31(2):89-95.
    PMID: 34347983 DOI: 10.1615/CritRevEukaryotGeneExpr.2021037996
    Among various epithelial-to-mesenchymal transition (EMT)-related transcription factors (TFs), altered expression levels of Snail-1, Snail-2/Slug, Twist, and ZEB1 have shown a significant association in different cancers having a higher risk of metastasis. However, their role in the circulation of endometriosis patients is not well understood. Hence, the present study was designed to evaluate the crucial role of these TFs in defining the molecular pathogenesis for endometriosis progression and differentiation from control subjects. The qualitative and quantitative expression analysis of Snail-1, Snail-2/Slug, Twist, and ZEB1 were analyzed in peripheral blood samples of 75 different stages of endometriosis patients and compared with 50 control subjects. Total RNA was extracted and converted into complementary DNA (cDNA) for relative quantification of each gene transcript using SYBRGreen-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). The Livak method of relative quantification was used for calculating the fold change in each TF compared with endogenous control. All four selected TFs showed significantly upregulated expression levels in endometriosis patients compared with control subjects. A three-fold increase was observed for Snail-1 (p = 0.0001), and a two-fold increase was observed for Snail-2 (p = 0.01), Twist (p = 0.0002), and ZEB1 (p = 0.001) in stage III and IV compared with stage I and II of endometriosis patients. The present study revealed that EMT-related TFs play a crucial role in the pathogenesis and differentiating different stages of endometriosis patients through expression analysis of specific molecular cascades using non-invasive tools.
    MeSH terms: Endometriosis/genetics*; Female; Humans; Nuclear Proteins/genetics*; Biomarkers/metabolism; Case-Control Studies; Twist Transcription Factor/genetics*; Epithelial-Mesenchymal Transition*; Snail Family Transcription Factors/genetics*; Zinc Finger E-box-Binding Homeobox 1/genetics*
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