Browse publications by year: 2024

  1. Tan YJ, Ong SC
    Value Health, 2024 Dec;27(12):1762-1770.
    PMID: 39127252 DOI: 10.1016/j.jval.2024.07.016
    OBJECTIVES: The Assessment of Quality of Life - 6 Dimensions (AQoL-6D), a generic preference-based measure, is an appealing alternative to EQ-5D-5L for assessing health status in patients with chronic heart failure (HF), given its expanded scope. However, without a Malaysian value set, the AQoL-6D cannot generate health state utility values (HSUVs) to support local economic evaluations. This study intended to develop algorithms for predicting EQ-5D-5L HSUVs from AQoL-6D in an HF population.

    METHODS: Cross-sectional data from a multicenter cohort of 419 HF outpatients were used. Both direct and indirect mapping approaches were attempted using 5 sets of explanatory variables and 8 models (ordinary least squares, Tobit, censored least absolute deviations, generalized linear model, 2-part model [TPM], beta regression-based model, adjusted limited dependent variable mixture model, and multinomial ordinal regression [MLOGIT]). The models' predictive performance was assessed through 10-fold cross-validated mean absolute error [MAE] and root mean squared error [RMSE]). Potential prediction bias was also examined graphically. The best-performing models, with the lowest RMSE and no bias, were then identified.

    RESULTS: Among the models evaluated, TPM, which included age, sex, and 5 AQoL-6D dimension scores as predictors, appears to be the best-performing model for directly predicting EQ-5D-5L HSUVs from AQoL-6D. TPM yielded the lowest MAE (0.0802) and RMSE (0.1116), and demonstrated predictive accuracy for HSUVs >0.2 without significant bias. A MLOGIT model developed for response mapping had suboptimal predictive accuracy.

    CONCLUSIONS: This study developed potentially useful mapping algorithms for generating Malaysian EQ-5D-5L HSUVs from AQoL-6D responses among patients with HF when direct EQ-5D-5L data are unavailable.

    MeSH terms: Aged; Algorithms; Chronic Disease; Cross-Sectional Studies; Female; Health Status; Humans; Malaysia; Male; Middle Aged; Quality of Life*; Surveys and Questionnaires
  2. Chan YL, Tang SN, Osman CP, Chee CF, Tay ST
    Microb Pathog, 2024 Aug 31;195:106886.
    PMID: 39182855 DOI: 10.1016/j.micpath.2024.106886
    Given the ability of Staphylococcus aureus to form biofilms and produce persister cells, making infections difficult to treat with antibiotics alone, there is a pressing need for an effective antibiotic adjuvant to address this public health threat. In this study, a series of quinone derivatives were evaluated for their antimicrobial and antibiofilm activities against methicillin-susceptible and methicillin-resistant S. aureus reference strains. Following analyses using broth microdilution, growth curve analysis, checkerboard assay, time-kill experiments, and confocal laser scanning microscopy, menadione was identified as a hit compound. Menadione exhibited a notable antibacterial profile (minimum inhibitory concentration, MIC = 4-16 μg/ml; minimum bactericidal concentration, MBC = 256 μg/ml) against planktonic S. aureus and its biofilms (minimum biofilm inhibitory concentration, MBIC50 = 0.0625-0.25 μg/ml). When combined with oxacillin, erythromycin, and vancomycin, menadione exhibited a synergistic or additive effect against planktonic cells and biofilms of two S. aureus reference strains and six clinical isolates, highlighting its potential as a suitable adjuvant for further development against S. aureus biofilm-associated infections.
  3. Díaz PA, Araya M, Cantarero B, Miranda C, Varela D, Figueroa RI, et al.
    Environ Pollut, 2024 Aug 28;361:124844.
    PMID: 39209054 DOI: 10.1016/j.envpol.2024.124844
    In late summer and early autumn 2022, an intense bloom of Protoceratium reticulatum-the main yessotoxin (YTX) producer along Chilean coasts and a major threat to artisanal fisheries, the aquaculture industry, and environmental health-was recorded in the Patagonian fjord system. The high YTX levels (>3.75 mg kg-1) resulted in the first ban of shellfish collection in Chile. At Puyuhuapi Fjord, a global "hotspot" of harmful algal bloom events, the cell density of P. reticulatum determined in integrated tube samples (0-10 m) at the end of April 2022 reached 407,000 cells L-1. At the same time, YTX levels well exceeded the regulatory limit by roughly 2.5-fold, with concentrations as high as 9.42 mg kg-1 measured in native populations of the blue mussel Mytilus chilensis. Five different YTX analogues, 45-OH-YTX, COOH-45-keto-YTX, COOH-45-OH-YTX, COOH-YTX, and 45,55-diOH-YTX, were also detected in relevant amounts. While the ban lasted close to 3 months, accumulation and detoxification processes were monitored over a 1-year period. This study assessed the implications of high levels of YTXs and their analogues on the local economy and ecosystem health, given the increase in P. reticulatum blooms predicted for NW Patagonia in the context of a changing climate.
  4. Farghadani R, Lim HY, Abdulla MA, Rajarajeswaran J
    Bioorg Chem, 2024 Nov;152:107730.
    PMID: 39216194 DOI: 10.1016/j.bioorg.2024.107730
    Breast cancer is the most prevalent cancer among women globally, with triple-negative breast cancer (TNBC) associated with poor prognosis and low five-year survival rates. Schiff base compounds, known for their extensive pharmacological activities, have garnered significant attention in cancer drug research. This study aimed to evaluate the anticancer potential of a novel β-diiminato compound and elucidate its mechanism of action. The compound's effect on cell viability was assessed using MTT assays in breast cancer cell lines including MCF-7 and MDA-MB-231. Cytotoxic effects were further analyzed using trypan blue exclusion and lactate dehydrogenase (LDH) release assays. In order to assess the mechanism of inhibitory activity and mode of cell death induced by this compound, flow cytometry of cell cycle distribution and apoptosis analysis were carried out. Apoptosis incidence was initially assessed through cell and nuclear morphological changes (Hoechst 33342/Propidium iodide (PI) staining) and further confirmed by Annexin V/PI staining and flow cytometry analysis. In addition, the effect of this compound on the disruption of mitochondrial membrane potential (MMP) and generation of the reactive oxygen species (ROS) was determined using the JC-1 indicator and DCFDA dye, respectively. The results demonstrated that the 24 h treatment with β-diiminato compound significantly suppressed the viability of MDA-MB-231 and MCF-7 cancer cells in a dose-dependent manner with the IC50 value of 2.41 ± 0.29 and 3.51 ± 0.14, respectively. The cytotoxic effect of the compound was further confirmed with a dose-dependent increase in the number of dead cells and enhanced LDH level in the culture medium. This compound exerted its anti-proliferative effect by G2/M phase cell growth arrest in MDA-MB-231 breast cancer cells and induced apoptosis-mediated cell death, which involved characteristic changes in cell and nuclear morphology, phosphatidylserine externalization, mitochondrial membrane depolarization, and increased ROS level. Neither hepatotoxicity nor nephrotoxicity was detected in the biochemical and histopathological analysis confirming the safety characterization of this compound usage. Therefore, the results significantly confirmed the potential anticancer activity of a novel β-diiminato compound, as evidenced by the induction of cell cycle arrest and apoptosis, which might be driven by the ROS‑mediated mitochondrial death pathway. This compound can be a promising candidate for future anticancer drug design and TNBC treatment, and further preclinical and clinical studies are warranted.
    MeSH terms: Animals; Dose-Response Relationship, Drug*; Drug Screening Assays, Antitumor*; Female; Humans; Structure-Activity Relationship; Molecular Structure; Reactive Oxygen Species/metabolism; Cell Line, Tumor; Mice; Membrane Potential, Mitochondrial/drug effects
  5. Kamaruzaman N, Kansha Y, Abbas A, Abdul Manaf N
    Waste Manag, 2024 Aug 29;189:219-229.
    PMID: 39216364 DOI: 10.1016/j.wasman.2024.08.020
    This research aims to evaluate the techno-economic viability and commercial potential of biomass gasification across different capacities. Sensitivity analysis was conducted based on an established downdraft gasifier model using Aspen Plus. Results underscored the significant impact of gasification temperature and equivalence ratio (ER) on syngas composition, low heating value (LHV), and cold gas efficiency (CGE). Among the feedstocks tested, coconut shell emerged as a feasible feedstock, yielding syngas with an LHV of 8.93 MJ/Nm3 and achieving a CGE of up to 71.12 %. Optimal gasification temperatures ranged between 750 °C to 1,000 °C, with peak ER falling within 0.1 to 0.3. Economic analysis revealed that smaller-scale operations like Plant A resulted in a negative net present value of - US$0.63 million, indicating unfavorable investments. The internal rate of return notably increased from 9.53 % for Plant B compared to -2.56 % for Plant A (20 kW). Plant D, with larger capacity of 20 MW, showed an impressive payback period of less than two years (1.69 years). Medium to large-scale plants such as Plant C (2 MW) and Plant D demonstrated greater economic resilience, with Plant D achieving a significantly lower levelized cost of electricity of US$ 0.19/kWh compared to Plant A at US$ 0.86/kWh. It was noted that the impact of capital costs, operating expenses, and revenue variations is less pronounced at larger scales. The findings from this study shed light on the feasibility of biomass gasification for power generation as a viable option, thereby unlocking the potential for its large-scale commercialization.
  6. Thapa R, Ahmad Bhat A, Shahwan M, Ali H, PadmaPriya G, Bansal P, et al.
    Brain Res, 2024 Dec 15;1845:149202.
    PMID: 39216694 DOI: 10.1016/j.brainres.2024.149202
    Alzheimer's Disease (AD) is a progressive neurological disease associated with behavioral abnormalities, memory loss, and cognitive impairment that cause major causes of dementia in the elderly. The pathogenetic processes cause complex effects on brain function and AD progression. The proper protein homeostasis, or proteostasis, is critical for cell health. AD causes the buildup of misfolded proteins, particularly tau and amyloid-beta, to break down proteostasis, such aggregates are toxic to neurons and play a critical role in AD pathogenesis. The rise of cellular senescence is accompanied by aging, marked by irreversible cell cycle arrest and the release of pro-inflammatory proteins. Senescent cell build-up in the brains of AD patients exacerbates neuroinflammation and neuronal degeneration. These cells senescence-associated secretory phenotype (SASP) also disturbs the brain environment. When proteostasis failure and cellular senescence coalesce, a cycle is generated that compounds each other. While senescent cells contribute to proteostasis breakdown through inflammatory and degradative processes, misfolded proteins induce cellular stress and senescence. The principal aspects of the neurodegenerative processes in AD are the interaction of cellular senescence and proteostasis failure. This review explores the interconnected roles of proteostasis disruption and cellular senescence in the pathways leading to neurodegeneration in AD.
    MeSH terms: Aging/metabolism; Aging/pathology; Aging/physiology; Animals; Brain/metabolism; Brain/pathology; Humans; Neurons/metabolism; Neurons/pathology; Amyloid beta-Peptides/metabolism; Cell Aging*; Neurodegenerative Diseases/metabolism; Neurodegenerative Diseases/pathology; Neurodegenerative Diseases/physiopathology
  7. Li D, Fan X, Meng L
    Sci Rep, 2024 Aug 31;14(1):20287.
    PMID: 39217173 DOI: 10.1038/s41598-024-70908-3
    Assessing and cultivating students' HOTS are crucial for interior design education in a blended learning environment. However, current research has focused primarily on the impact of blended learning instructional strategies, learning tasks, and activities on the development of HOTS, whereas few studies have specifically addressed the assessment of these skills through dedicated scales in the context of blended learning. This study aimed to develop a comprehensive scale for assessing HOTS in interior design major students within the context of blended learning. Employing a mixed methods design, the research involved in-depth interviews with 10 education stakeholders to gather qualitative data, which informed the development of a 66-item soft skills assessment scale. The scale was administered to a purposive sample of 359 undergraduate students enrolled in an interior design program at a university in China. Exploratory and confirmatory factor analyses were also conducted to evaluate the underlying factor structure of the scale. The findings revealed a robust four-factor model encompassing critical thinking skills, problem-solving skills, teamwork skills, and practical innovation skills. The scale demonstrated high internal consistency (Cronbach's alpha = 0.948-0.966) and satisfactory convergent and discriminant validity. This scale provides a valuable instrument for assessing and cultivating HOTS among interior design major students in blended learning environments. Future research can utilize a scale to examine the factors influencing the development of these skills and inform instructional practices in the field.
    MeSH terms: Adult; China; Educational Measurement/methods; Female; Humans; Learning*; Male; Problem Solving; Surveys and Questionnaires; Students*; Thinking*; Universities; Young Adult
  8. Hai T, Basem A, Alizadeh A, Sharma K, Jasim DJ, Rajab H, et al.
    Sci Rep, 2024 Aug 31;14(1):20271.
    PMID: 39217234 DOI: 10.1038/s41598-024-71027-9
    Suspensions containing microencapsulated phase change materials (MPCMs) play a crucial role in thermal energy storage (TES) systems and have applications in building materials, textiles, and cooling systems. This study focuses on accurately predicting the dynamic viscosity, a critical thermophysical property, of suspensions containing MPCMs and MXene particles using Gaussian process regression (GPR). Twelve hyperparameters (HPs) of GPR are analyzed separately and classified into three groups based on their importance. Three metaheuristic algorithms, namely genetic algorithm (GA), particle swarm optimization (PSO), and marine predators algorithm (MPA), are employed to optimize HPs. Optimizing the four most significant hyperparameters (covariance function, basis function, standardization, and sigma) within the first group using any of the three metaheuristic algorithms resulted in excellent outcomes. All algorithms achieved a reasonable R-value (0.9983), demonstrating their effectiveness in this context. The second group explored the impact of including additional, moderate-significant HPs, such as the fit method, predict method and optimizer. While the resulting models showed some improvement over the first group, the PSO-based model within this group exhibited the most noteworthy enhancement, achieving a higher R-value (0.99834). Finally, the third group was analyzed to examine the potential interactions between all twelve HPs. This comprehensive approach, employing the GA, yielded an optimized GPR model with the highest level of target compliance, reflected by an impressive R-value of 0.999224. The developed models are a cost-effective and efficient solution to reduce laboratory costs for various systems, from TES to thermal management.
  9. Banovic M, Putnik S, Da Costa BR, Penicka M, Deja MA, Kotrc M, et al.
    Eur Heart J, 2024 Sep 01.
    PMID: 39217448 DOI: 10.1093/eurheartj/ehae585
    BACKGROUND AND AIMS: The question of when and how to treat truly asymptomatic patients with severe aortic stenosis (AS) and normal left ventricular (LV) systolic function is still subject to debate and ongoing research. Here, the results of extended follow-up of the AVATAR trial are reported (NCT02436655, clinical trials.gov).

    METHODS: The AVATAR trial randomly assigned patients with severe, asymptomatic AS and LV ejection fraction ≥50% to undergo either early surgical aortic valve replacement (AVR) or conservative treatment with watchful waiting strategy. All patients had negative exercise stress testing. The primary hypothesis was that early AVR will reduce a primary composite endpoint comprising all-cause death, acute myocardial infarction, stroke or unplanned hospitalization for heart failure (HF), as compared to conservative treatment strategy.

    RESULTS: A total of 157 low-risk patients (mean age 67 years, 57% men, mean Society of Thoracic Surgeons score 1.7%) were randomly allocated to either early AVR group (n=78) or conservative treatment group (n=79). In an intention-to-treat analysis, after a median follow-up of 63 months, the primary composite endpoint outcome event occurred in 18/78 patients (23.1%) in the early surgery group and in 37/79 patients (46.8%) in the conservative treatment group (hazard ratio [HR] early surgery vs. conservative treatment 0.42; 95% confidence interval [CI] 0.24-0.73, p=0.002). The Kaplan-Meier estimates for individual endpoints of all-cause death and HF hospitalization were significantly lower in the early surgery compared with the conservative group (HR 0.44; 95% CI 0.23-0.85, p=0.012 for all-cause death, and HR 0.21; 95% CI 0.06-0.73, p=0.007 for HF hospitalizations).

    CONCLUSIONS: The extended follow-up of the AVATAR trial demonstrates better clinical outcomes with early surgical AVR in truly asymptomatic patients with severe AS and normal LV ejection fraction compared with patients treated with conservative management on watchful waiting.

    TRIAL REGISTRATION NUMBER: NCT02436655 (ClinicalTrials.gov).

  10. Ismet S, Hehsan MR
    J Perianesth Nurs, 2024 Aug 29.
    PMID: 39217526 DOI: 10.1016/j.jopan.2024.05.025
    Argininemia is a rare autosomal recessive metabolic disorder characterized by a deficiency of arginase, a vital enzyme in the urea cycle. This metabolic defect results in the accumulation of arginine and its metabolites, leading to hyperammonemia and associated neurological symptoms. We present a case detailing the perioperative management of an 11-year-old male child diagnosed with argininemia undergoing circumcision. The perioperative management of patients with argininemia presents unique challenges due to the risk of hyperammonemia and neurological decompensation triggered by physiological stress, fasting, and the catabolic state associated with surgery. This case report highlights the importance of individualized anesthetic strategies for patients with rare metabolic disorders like argininemia. A multidisciplinary approach involving collaboration among anesthesiologists, endocrinologists, dietitians, and surgeons is essential to ensuring a safe perioperative experience for these patients. Further research is essential to refine perioperative protocols and optimal anesthetic interventions for individuals with argininemia undergoing surgical procedures.
  11. Guerra-Farfan E, Borges FK, Bhandari M, Garcia-Sanchez Y, Nuoez JH, Mestre-Torres J, et al.
    Injury, 2024 Aug 20;55(11):111827.
    PMID: 39217648 DOI: 10.1016/j.injury.2024.111827
    BACKGROUND: Hip fractures carry a substantial risk of complications and death. This study aimed to report the 90-day incidence of mortality, major perioperative complications and in-hospital timelines after a hip fracture in the Spanish HIP ATTACK-1 trial cohort, comparing with the non-Spanish cohort.

    METHODS: Prospective cohort study of Spanish patients nested in the HIP ATTACK-1 trial. The HIP ATTACK-1 was an international, randomized, controlled trial (17 countries, 69 hospitals, 7 in Spain, highest recruiting country). Patients were randomized to either accelerated surgery (goal of surgery within 6 h of diagnosis) or standard care. Participants were ≥45 years of age who presented with a low-energy hip fracture requiring surgery.

    RESULTS: Among 534 patients in the Spanish cohort, 69 (12.9 %) patients died at 90 days follow-up, compared to 225 (9.2 %) in the non-Spanish cohort (p = 0.009), mostly due to higher nonvascular related mortality. A composite of major postoperative complication occurred in 126 patients (23.6 %). The most common perioperative complications were myocardial injury (189 patients, 35.4 %), infection with no sepsis (86 patients, 16.1 %) and perioperative delirium (84 patients, 15.7 %); all these complication rates in Spain were significantly higher than the non-Spanish patients (29.2 % p = 0.005; 11.9 % p = 0.008 and 9.2 % p < 0.0001, respectively). Spanish cohort patients were older and had more comorbidities than the non-Spanish cohort, evidencing their greater frailty at baseline. Among Spanish patients, the median time from hip fracture diagnosis to surgery was 30.0 h (IQR 21.1-53.9) in the standard-care group, with 68.8 % of patients receiving surgery within 48 h of diagnosis. This median time was lower in the non-Spanish cohort (22.8 h, IQR 9.5-37.0), where 82.1 % of patients were operated within 48 h.

    CONCLUSIONS: In the HIP ATTACK-1 trial, 1 in 8 patients died 90 days after a hip fracture in Spain. The most common complication after a hip fracture was myocardial injury, followed by infection and delirium. Spanish patients had worse outcomes than non-Spanish patients. Research needs to focus on new interventions such as accelerated surgery and perioperative troponin measurement with the appropriate investment of resources, to prevent and identify early these complications with a goal of improving mortality for this high-risk population.

    LEVEL OF EVIDENCE: II.

  12. Chan KK, Pereira AF, Valente AI, Tavares APM, Coutinho JAP, Ooi CW
    Int J Biol Macromol, 2024 Oct;277(Pt 4):134443.
    PMID: 39217672 DOI: 10.1016/j.ijbiomac.2024.134443
    Laccases hold great potential for biotechnological applications, particularly in environmental pollutant remediation. Laccase activity is governed by the solvent environment, and ionic liquids (ILs) emerge as a versatile solvent for activation or stabilization of enzymes. Herein, effects of cholinium-based ILs formulated with carboxylic acids, inorganic acid, and amino acids as anionic species, on the catalytic activity of laccase from Trametes versicolor were investigated by experimental and computational approaches. Experimental results showed that laccase activity was enhanced by 21.39 % in 0.5 M cholinium dihydrogen citrate ([Cho][DHC]), in relation to the laccase activity in phosphate buffer medium. However, cholinium aminoate ILs negatively affected laccase activity, as evidenced by the partial deactivation of laccase in both cholinium glycinate and cholinium phenylalaninate, at concentrations of 0.1 M and 0.5 M, respectively. Molecular dynamics studies revealed that the enhancement of laccase activity in [Cho][DHC] might be attributed to the highly stabilized and compact structure of laccase, facilitating a better internal electron transfer during the laccase-substrate interactions. Enhanced catalytic performance of laccase in [Cho][DHC] was postulated to be driven by the high accumulation level of dihydrogen citrate anions around laccase's surface. [Cho][DHC] holds great promise as a cosolvent in laccase-catalyzed biochemical reactions.
    MeSH terms: Choline/chemistry; Polyporaceae; Solvents/chemistry; Trametes/enzymology; Molecular Dynamics Simulation*
  13. Oh AL, Makmor-Bakry M, Islahudin F, Ting CY, Chan SK, Tie ST
    Res Social Adm Pharm, 2024 Dec;20(12 Pt A):1102-1109.
    PMID: 39218734 DOI: 10.1016/j.sapharm.2024.08.091
    BACKGROUND: Tuberculosis (TB) treatment interruption poses risks of antimicrobial resistance, potentially leading to treatment failure and mortality. Addressing the risk of early treatment interruption is crucial in tuberculosis care and management to improve treatment outcomes and curb disease transmission.

    OBJECTIVES: This study aimed to identify risk factors of TB treatment interruption and construct a predictive scoring model that enables objective risk stratification for better prediction of treatment interruption.

    METHODS: A multicentre retrospective cohort study was conducted at public health clinics in Sarawak, Malaysia over 11 months from March 2022 to January 2023, involving adult patients aged ≥18 years with drug-susceptible TB diagnosed between 2018 and 2021. Cumulative missed doses or discontinuation of TB medications for ≥2 weeks, either consecutive or non-consecutive, was considered as treatment interruption. The model was developed and internally validated using the split-sample method. Multiple logistic regression analysed 18 pre-defined variables to identify the predictors of TB treatment interruption. The Hosmer-Lemeshow test and area under the receiver operating characteristic curve (AUC) were employed to evaluate model performance.

    RESULTS: Of 2953 cases, two-thirds (1969) were assigned to the derivation cohort, and one-third (984) formed the validation cohort. Positive predictors included smoking, previously treated cases, and adverse drug reactions, while concurrent diabetes was protective. Based on the validation dataset, the model demonstrated good calibration (P = 0.143) with acceptable discriminative ability (AUC = 0.775). A cutoff score of 2.5 out of 11 achieved a sensitivity of 81 % and a specificity of 64.4 %. Risk stratification into low (0-2), medium (3-5), and high-risk (≥6) categories showed ascending interruption rates of 5.3 %, 18.1 %, and 41.3 %, respectively (P 

    MeSH terms: Adult; Aged; Female; Humans; Malaysia; Male; Middle Aged; Retrospective Studies; Risk Factors; Cohort Studies; Risk Assessment; Young Adult
  14. Mohamad K, Zainal H, Abdul Rahim NA, Tengku Hussain TH
    J Nephrol, 2024 Dec;37(9):2551-2559.
    PMID: 39218996 DOI: 10.1007/s40620-024-02076-6
    BACKGROUND: Managing children with frequent relapses or steroid-dependent nephrotic syndrome poses challenges due to recurrent relapses necessitating prolonged steroid exposure, thus increasing susceptibility to long-term complications. Identifying those at risk of poor response to steroid therapy may be helpful to guide timely intervention with steroid-sparing agents. This study aimed to identify factors associated with steroid-sparing agent needs in children with frequent relapses or steroid-dependent nephrotic syndrome.

    METHODS: A retrospective multicenter cohort study was conducted by reviewing the medical records of children with idiopathic nephrotic syndrome treated between 2006 and 2023. Cox proportional regression analyzed prognostic factors for steroid-sparing agent requirements in children with frequent relapses or steroid-dependent nephrotic syndrome. The time-to-event analysis utilizing the Kaplan-Meier estimate examined the proportion of children needing steroid-sparing agents after diagnosis.

    RESULTS: Medical records of 121 children (85 males) diagnosed with idiopathic nephrotic syndrome at a median age of 4.5 years (range 1.3-12.8) were reviewed over a median follow-up of 3.7 years (range 1.0-15.0). Time to subsequent relapse post-frequent relapses or steroid-dependent nephrotic syndrome diagnosis (at 3-month threshold) emerged as the sole significant predictor of steroid-sparing agent requirement, adjusted hazard ratio (aHR) = 2.26, 95% confidence interval (CI) 1.26-4.05. Kaplan-Meier analysis indicated that an earlier first relapse (

    MeSH terms: Child; Child, Preschool; Female; Glucocorticoids/therapeutic use; Humans; Infant; Male; Recurrence*; Retrospective Studies; Risk Factors; Time Factors; Proportional Hazards Models; Treatment Outcome; Kaplan-Meier Estimate
  15. Mo Y, Zhou L, Fu S, Yang H, Lin B, Zhang J, et al.
    PMID: 39219225 DOI: 10.1080/10934529.2024.2396728
    High level of aluminum content in Enteromorpha prolifera posed a growing threat to both its growth and human health. This study focused on exploring the factors, impacts, and process of removing aluminum from Enteromorpha prolifera using humic acid. The results showed that under experimental conditions of 0.0330 g·L-1 humic acid concentration, pH 3.80, 34 °C, and a duration of 40 min, the removal rate was up to 80.18%. The levels of major flavor components, proteins, and amino acids in Enteromorpha prolifera increased significantly after treatment, while polysaccharides and trace elements like calcium and magnesium decreased significantly. Infrared spectroscopy demonstrated that the main functional groups involved in binding with Al3+ during humic acid adsorption were hydroxyl, carboxyl, phenol, and other oxygen-containing groups. The adsorption process of Al3+ by humic acid was a spontaneous phenomenon divided into three key stages: fast adsorption, slow adsorption, and adsorption equilibrium, which resulted from both physical and chemical adsorption effects. This study provided a safe and efficient method in algae metal removal.
  16. Tiong SL, Aida WN, I R, Hitam S
    Cureus, 2024 Jul;16(7):e65797.
    PMID: 39219903 DOI: 10.7759/cureus.65797
    Tuberculosis (TB) is endemic in Malaysia but rarely affects the middle ear cleft. Common presentations of TB mastoiditis include unilateral, painless otorrhea, multiple small perforations of the tympanic membrane, and facial nerve palsy, although these symptoms can vary among patients. The diagnosis of TB mastoiditis is challenging due to its rarity and its similar presentation to common bacterial ear infections. This often leads to missed diagnoses, resulting in significant delays in treatment and potential complications. CT scans and histopathological examinations are crucial for diagnosing TB mastoiditis. Real-time polymerase chain reaction offers higher sensitivity and specificity compared to conventional methods for detecting Mycobacterium tuberculosis. TB infection should be considered in cases of otitis media that do not respond well to empirical antibiotic therapy. It is essential to send appropriate samples for TB testing to ensure timely diagnosis and treatment. This case report highlights the diagnostic challenges and complications encountered in a 22-year-old immunocompromised woman with TB mastoiditis.
  17. V Rajendran K, Baharin F, Md Said H
    Cureus, 2024 Jul;16(7):e65907.
    PMID: 39219917 DOI: 10.7759/cureus.65907
    The presence of natal and neonatal teeth is a rare anomaly that can lead to various complications. This case report aims to highlight the potential delayed sequelae that can arise following the extraction of natal teeth. A boy aged three years and two months was referred for pain and an abscess on his lower anterior teeth. He had two natal teeth at birth, which were extracted on the second day of life due to excessive mobility and profound discomfort to the mother during breastfeeding. Surprisingly, he presented with pain in the same area where the teeth were extracted previously. Upon examination, residual root-like structures were observed at the sites of teeth 71 and 81, with an abscess noted on tooth 81. The radiographic assessment confirmed the presence of residual roots, prompting the extraction. History, clinical, radiographic, and histopathological evaluations strongly validate the diagnosis of residual natal teeth. Gentle curettage of the socket should be performed whenever possible following the extraction of natal teeth to prevent complications. Although residual tooth formation is uncommon, periodic follow-up is important to monitor for any potential problem and observe adjacent teeth eruption.
  18. Zhin Liang C, Mohamed Khalil AF, Kamarul Arif N, Syafiq S, Muhamad Ariffin MH
    Cureus, 2024 Jul;16(7):e65909.
    PMID: 39219936 DOI: 10.7759/cureus.65909
    Fracture-related infection (FRI) is a challenging complication in open fractures. It can cause major disability to patients and a burden to the public health sector. A multidisciplinary approach is required to eradicate infection and improve the quality of life for patients. We present a case of an FRI in an open fracture of the distal femur treated using a supracutaneous locking plate, which is an uncommon technique. This technique yields excellent outcomes in controlling local infection and providing satisfactory stability, especially for a peri-articular distal femur fracture with FRI. Therefore, supracutaneous plating using a locking plate can be considered an alternative option to conventional external fixations in managing FRIs.
  19. Nur Zafirah Z, Abdul Hadi A
    PMID: 39220235 DOI: 10.51866/cr.644
    Hypopigmented mycosis fungoides (MF) is a rare variant of cutaneous T-cell lymphoma, a type of extranodal non-Hodgkin lymphoma. This report presents the case of a 9-year-old boy with a 2-year history of asymptomatic, hypopigmented skin lesions that were resistant to topical treatment. He was initially treated for a fungal skin infection and had received multiple courses of topical antifungals and steroids but showed no improvement, which led to further evaluation and a referral to a dermatologist. A skin biopsy was performed, and the diagnosis of hypopigmented MF was confirmed through skin histopathology and immunohistochemistry study. His lesions responded well to cycles of narrowband ultraviolet B phototherapy, showing almost complete clearance after 4 months without any side effects.
  20. Ahmed Abrizan H, Ikhwan SM, Wan Mokhzani WM, Siti Rahmah HMI, Yahya MM, Syed Hassan SAA
    PMID: 39220236 DOI: 10.51866/cr.624
    Urine amylase levels are usually used to diagnose acute pancreatitis. However, there are reported cases where urine amylase levels are slightly increased in individuals without pancreatitis. Herein, we report the case of a young lady who presented with acute abdominal pain for 3 days. Her urine amylase level was 1717 U/L upon admission, and her condition was initially treated as acute pancreatitis. Unfortunately, the patient demonstrated abdominal guarding after 24 h; thus, urgent computed tomography (CT) was performed. CT revealed the presence of a dilated small bowel. She underwent emergency laparotomy, wherein a gangrenous small bowel with no evidence of saponification at the lesser sac was noted. Due to the non-specific nature of hyperamylasaemia, an alternative diagnosis other than acute pancreatitis should be considered if the clinical symptoms are not suggestive of pancreatitis or the condition worsens despite conservative management.
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