Browse publications by year: 2025

  1. Ng KT, Kwok PE, Lim WE, Teoh WY, Hasan MS, Zainal Abidin MF
    Braz J Anesthesiol, 2025;75(1):844580.
    PMID: 39615751 DOI: 10.1016/j.bjane.2024.844580
    OBJECTIVES: Methylene blue exerts its vasopressor properties by inhibiting nitric oxide-mediated vasodilation. Recent studies have advocated the use of methylene blue as a rescue therapy for patients with septic shock. The primary aim was to investigate the effect of methylene blue on the mean arterial pressure among adult patients with septic shock.

    METHODS: Databases of MEDLINE, EMBASE, and CENTRAL were searched from their inception date until October 2023. Randomized Clinical Trials (RCT) comparing methylene blue and placebo in adults with septic shock were included.

    RESULTS: Our systematic review included 5 studies (n = 257) for data analysis. As compared to the placebo, our pooled analysis showed that methylene blue significantly increased mean arterial pressure (MD: 1.34 mmHg, 95% CI 0.15 to 2.53, p = 0.03, level of evidence: very low). Patients who were given methylene blue were associated with statistically lower mortality rate (OR = 0.49, 95% CI 0.27 to 0.88, p = 0.02, level of evidence: low), reduced serum lactate levels (MD: -0.76 mmoL.L-1, 95% CI -1.22 to -0.31, p = 0.0009, level of evidence: low), reduced length of hospital stay (MD: -1.94 days, 95% CI -3.79 to -0.08, p = 0.04, level of evidence: low), and increased PaO2/FiO2 (MD: 34.78, 95% CI 8.94 to 60.61, p = 0.008, level of evidence: low).

    CONCLUSIONS: This meta-analysis demonstrated that methylene blue administration was associated with an increased in mean arterial pressure and PaO2/FiO2 ratio, along with a reduction in mortality rates, serum lactate levels, and length of hospital stay. However, substantial degree of heterogeneity and inadequate number of studies with low level of evidence warrant future adequately powered RCTs to affirm our results.

    MeSH terms: Adult; Humans; Length of Stay; Vasoconstrictor Agents/administration & dosage; Vasoconstrictor Agents/therapeutic use; Randomized Controlled Trials as Topic*; Lactic Acid/blood; Arterial Pressure/drug effects
  2. Munir R, Khan IU, Kamal Y, Asghar S, Irfan M, Alshammari A, et al.
    Colloids Surf B Biointerfaces, 2025 Mar;247:114407.
    PMID: 39616935 DOI: 10.1016/j.colsurfb.2024.114407
    Arthritic disease is one of the most common diseases in adults and a leading cause of joint degeneration. Dexibuprofen (DEX) is routinely used for the treatment of rheumatoid arthritis, acute postoperative pain, primary dysmenorrheal, and in lower back pain. However, it is poorly water soluble with compromised bioavailability, and hence has limited therapeutic activity. In order to overcome these issues, we studied the formulation and characterization of nanoemulsion based system i.e nanoemulgel of DEX. This study aimed to prepare topical nanoemulgel containing 2 % DEX and solubility-enhanced DEX via ternary inclusion complexation (DEX-SE-T) and to compare it with commercially available 5 % Ibuprofen gel as there is no topical formulation of DEX is available in the market currently. A pseudoternary phase diagram was constructed using the spontaneous water titration method. Blank and drug-loaded nanoemulgel were prepared using a high-speed homogenization method. All the formulations were evaluated in terms of particle size, pH, conductivity, viscosity, zeta potential, and ex vivo drug permeation. DEX loaded nanoemulgel yield enhanced in vitro skin permeation than the commercially available 5 % ibuprofen gel. The optimized nanoemulgel formulation (DEX-SE-T) was tested in in vivo anti-inflammatory models including cotton pellets-induced abdominal granuloma (chronic inflammation) and carrageenan-induced paw edema (acute inflammation). DEX-SE-T loaded nanoemulgel has improved in vivo anti-inflammatory activity as compared to ibuprofen gel. DEX-SE-T could be a promising option for effective topical treatment of inflammatory conditions.
    MeSH terms: Administration, Cutaneous; Administration, Topical; Animals; Anti-Inflammatory Agents/administration & dosage; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/chemistry; Anti-Inflammatory Agents, Non-Steroidal/administration & dosage; Anti-Inflammatory Agents, Non-Steroidal/pharmacology; Anti-Inflammatory Agents, Non-Steroidal/chemistry; Carrageenan/chemistry; Edema/chemically induced; Edema/drug therapy; Male; Particle Size; Skin/drug effects; Skin/metabolism; Skin/pathology; Skin Absorption/drug effects; Solubility; Viscosity; Rats, Wistar; Rats; Nanoparticles/chemistry
  3. Chittapun S, Suwanmanee K, Kongsinkaew C, Pornpukdeewattana S, Chisti Y, Charoenrat T
    J Biotechnol, 2025 Feb;398:76-86.
    PMID: 39617332 DOI: 10.1016/j.jbiotec.2024.11.018
    The natural blue colorant C-phycocyanin (C-PC) has many potential applications but its poor heat stability limits its commercial use. This study compares the production and thermal stability of C-PC from two cyanobacteria: the thermophilic Thermosynechococcus sp. TUBT-T01 and the mesophilic Synechococcus cedrorum TISTR8589. Thermosynechococcus sp. produced nearly 1.9-fold more C-PC than S. cedrorum. Batch adsorption using a chromatographic cationic ion exchange resin (Streamline Direct HST1) was used to effectively purify the C-PC. The equilibrium adsorption capacity (Qeq) of the resin for C-PC was the highest at pH 5. At this pH, the Qeq for the thermophilic C-PC was 5.5 ± 0.1 mg mL⁻¹ , whereas for the mesophilic C-PC it was 1.5 ± 0.2 mg mL⁻¹ . Purification increased the concentration of the thermophilic C-PC by 5.9-fold, and that of mesophilic C-PC by 4.2-fold. The purity ratios of the final products from the two cyanobacteria were similar at ∼2.2. At 60 °C and pH 7, the C-PC of Thermosynechococcus sp. had ∼12-times longer half-life than the mesophilic C-PC; however, the productivity of the thermophilic C-PC was comparatively low because of a low biomass productivity of Thermosynechococcus sp.
    MeSH terms: Adsorption; Hot Temperature; Hydrogen-Ion Concentration; Kinetics
  4. Farshid S, Buckland BC, Shanmuganathan S, Low GK
    Respir Med, 2025 Jan;236:107891.
    PMID: 39617353 DOI: 10.1016/j.rmed.2024.107891
    BACKGROUND AND OBJECTIVE: Accurate severity assessment in acute asthma is vital to guide patient management and disposition. End-tidal carbon dioxide (EtCO2) has been proposed as a real-time measure for this purpose. This study aimed to systematically review literature on EtCO2 measurement in assessing the severity of acute asthma exacerbations.

    METHODS: Five databases were searched. Studies with patients of any age with acute asthma exacerbations and at least one clinical outcome measure were included. Studies on intubated patients and outpatients were excluded. Two independent reviewers screened abstracts and then full texts for eligibility.

    RESULTS: 1242 records were identified and 11 studies were included in the review. Three out of five studies found significant differences in capnography measures between patients eventually admitted and those discharged from the emergency department. Patients with lower initial EtCO2 were more likely to require hospital admission. Other components of the capnography waveform were associated with disposition, including a larger alpha angle and a lower ratio between phase III duration and respiratory rate being associated with hospital admission. Seven studies examined correlations between capnography measures and other markers of airway obstruction and weak or absent correlations were generally found. Three studies reported significant change in capnography measures after treatment.

    CONCLUSION: Lower EtCO2 may predict poorer outcome in acute asthma exacerbations. Other measures taken from the capnography waveform appear to be useful indicators of severity. Addressing patient selection issues and conducting prognostic accuracy studies of EtCO2 with clinical endpoints may provide meaningful evidence for clinical practice.

    MeSH terms: Acute Disease; Adult; Aged; Female; Hospitalization; Humans; Male; Middle Aged; Severity of Illness Index*; Disease Progression; Point-of-Care Systems; Respiratory Rate/physiology
  5. Elsayed N, How CW, Foo JB
    Eur J Pharm Biopharm, 2025 Feb;207:114599.
    PMID: 39617356 DOI: 10.1016/j.ejpb.2024.114599
    Zerumbone (ZER), a compound derived from the rhizome of Zingiber Zerumbet (L.) Smith, has demonstrated anti-inflammatory properties but suffers from poor water solubility, limiting its clinical application. While ZER's effects on lung inflammation are known, its role in lung fibrosis remains unexplored. Herein, ZER was encapsulated in pH-sensitive liposomes formulated with oleic acid, dipalmitoylphosphatidylcholine, and cholesterol to enhance ZER solubility and delivery to the acidic environment of lung fibrosis. The liposomes were optimized using Box-Behnken design, resulting in an average diameter of 87.8 ± 3.5 nm, a polydispersity index of 0.16 ± 0.2, and a zeta potential of -24 ± 0.32 mV. ZER release from the carrier followed zero-order kinetics and showed higher release in acidic settings. Cascade impactor and HPLC analyses confirmed that ZER liposome powder produced by freeze-drying reached stage 7, indicating effective delivery to deep lung regions. The uptake of ZER liposomes was concentration and pH-dependent, being higher in acidic conditions and greater in MRC-5 cells compared to A549 cells. Notably, ZER liposomes reduced cell migration and downregulated fibrotic markers such as fibronectin, MMP-2, and α-SMA in MRC-5 and A549 cells. This study suggests that ZER liposomes hold promise for treating lung fibrosis and merit further investigation.
    MeSH terms: Administration, Inhalation; Cholesterol/chemistry; Humans; Hydrogen-Ion Concentration; Liposomes*; Particle Size; Solubility; 1,2-Dipalmitoylphosphatidylcholine/chemistry; Oleic Acid/chemistry; Drug Liberation; A549 Cells
  6. Soluk-Tekkesin M, Bologna-Molina R, Magliocca K, van Heerden W, Robinson L, Bilodeau EA, et al.
    J Oral Pathol Med, 2025 Jan;54(1):76-79.
    PMID: 39617625 DOI: 10.1111/jop.13592
    BACKGROUND: The terminology surrounding developmental lesions in the oral cavity is widely applied, often leading to confusion in differentiating between developmental malformations and neoplasia. Odontogenic tumor classification includes both true neoplasms and malformations which make it very complex and dynamic.

    METHOD AND CONCLUSION: In this brief report, we will first discuss the concepts of malformations and neoplasia and then focusing on their relevance to odontogenic tumors, which impacts their classification and treatment, particularly mixed odontogenic lesions.

    MeSH terms: Diagnosis, Differential; Humans; Mouth Abnormalities/pathology; Terminology as Topic; Tooth Abnormalities/pathology
  7. Abel AG, Shamarina S, Hisham MNMN, Hafiz ARA
    Afr J Infect Dis, 2025;19(1):45-56.
    PMID: 39618539 DOI: 10.21010/Ajidv19i1.6
    BACKGROUND: Hepatitis B (HB), caused by the hepatitis B virus (HBV), remains a critical public health challenge in Nigeria. Globally, the HBV infects approximately 296 million people, significantly contributing to morbidity and mortality, with liver cirrhosis and liver cancer ranking as the 11th and 24th leading causes of death, respectively. This study aimed to determine the prevalence of HBV infection and the associated risk factors within the Lafia Metropolis, Nasarawa State, Nigeria.

    MATERIALS AND METHODS: A cross-sectional study was conducted from September to November 2023, utilizing a validated questionnaire to assess 461 randomly selected participants from four communities (Azuba, Bukan Sidi, Danka Sarki, and Doka), representing diverse sociodemographic profiles and varying degrees of exposure to risk factors associated with HBV infection. Data were analyzed using chi-square (χ2) tests with IBM SPSS statistical software version 28.0 at a significance level of p < 0.05.

    RESULTS: The findings showed that the overall prevalence of HBV infection was 7.8%. A significant association was observed between the prevalence of HBV infection and monthly income (p < 0.01). However, no associations were found with age, gender, educational level, marital status, occupation, or religion. Regarding risk factors, a significant association was found between HBV infection and awareness of one's HBV status (p < 0.03).

    CONCLUSION: These findings highlight the need for targeted public health interventions and policies aimed at reducing HBV transmission among high-risk sociodemographic groups in Lafia Metropolis. This approach could potentially reduce the burden of HBV and improve health outcomes in affected communities.

  8. Ladwig KH, Marten-Mittag B, Olliges E, Johar H, Atasoy S, Holdenrieder S, et al.
    J Affect Disord, 2025 Jan 15;369:174-181.
    PMID: 39321975 DOI: 10.1016/j.jad.2024.09.146
    BACKGROUND: Leptin, an adipokine suspected to play a role in coronary artery disease (CAD), may also be associated with deteriorated mental health. We investigated the prospective impact of recurrent depressed mood (RDM) on heightened plasma leptin levels in CAD patients.

    METHODS: Derived from the randomized SPIRR-CAD trial, plasma leptin were measured by the Human Leptin DuoSet ELISA at baseline in 539 patients (including 115 (21.3 %) women and 424 (78.7 %) men) and in 373 participants after 18-months follow up (T3). RDM was based on the clinical course from baseline to follow-up assessed by the Hamilton Depression Rating Scale (HAMD). Multivariate binary logistic regression models identified predictors for heightened leptin at T3.

    RESULTS: At baseline, highest leptin level (3rd tertile) was associated with type 2 diabetes (p = 0.009), heart failure symptoms (NYHA III) (p 

    MeSH terms: Aged; Depression/blood; Diabetes Mellitus, Type 2/blood; Female; Follow-Up Studies; Heart Failure/blood; Humans; Male; Middle Aged; Peptide Fragments/blood; Prospective Studies; Recurrence*; Risk Factors; Sex Factors; Body Mass Index; Natriuretic Peptide, Brain/blood
  9. Xie P, Wang F, Zhou J, Lee YY, Zhang Y, Zou S, et al.
    Food Chem, 2025 Feb 28;466:142196.
    PMID: 39612838 DOI: 10.1016/j.foodchem.2024.142196
    Compared to lard-based shortenings, diacylglycerol (DAG)-based shortenings have demonstrated beneficial effects, such as lowering blood lipids, and reducing postprandial blood glucose levels. In this study, different chain-length DAG oils were blended with lower melting point peanut oil DAG oil (PO-DAG-oil). The blend ratios for the three types of DAG-based shortenings were determined based on the solid fat content (SFC) of lard. Subsequently, 1 % of various emulsifiers were added, and the crystallization properties, rheological and textural characteristics, polymorphism, microstructure, water-absorbing capacity, and plasticity of the four shortening systems were examined. The emulsifiers found to be suitable for lard shortening, long chain fatty acid DAG (LCD-shortening), medium chain fatty acid DAG (MCD-shortening), and medium and long chain fatty acid DAG (MLCD-shortening) were Span60, PGFE, PGFE, and MAG, respectively. Cakes baked using DAG-based shortenings exhibited superior textural properties compared to those made with lard-based shortenings, supporting the application of high-melting-point DAG oils in shortening formulations.
    MeSH terms: Cooking; Fatty Acids/chemistry; Food Handling; Plant Oils/chemistry; Rheology; Emulsifying Agents/chemistry
  10. Jabbari S, Zakaria ZA, de Menezes IRA, Mohammadi S
    J Ethnopharmacol, 2025 Jan 13;339:119174.
    PMID: 39613005 DOI: 10.1016/j.jep.2024.119174
    ETHNOPHARMACOLOGICAL RELEVANCE: Fritillaria imperialis L. (Fabaceae), commonly known as "Laleh vazhgon", ethnomedicinally utilized in Iranian traditional medicine to treat joint pain, chronic daily headaches, and back pain.

    AIM OF THE STUDY: To investigate the antinociceptive, anti-neuropathic, and anti-migraine activities of Fritillaria imperialis bulbs essential oil (FIEO) as well as to uncover the potential mechanisms of action involved.

    MATERIALS AND METHODS: The antinociceptive activity of FIEO and its main constituent, Verticinone (Vt), was assessed using the formalin-induced paw licking assay. The potential mechanisms of antinociception were investigated through various antagonists. Additionally, their antineuropathic activity was examined using the cervical spinal cord contusion (CCS) technique and the possible role of Stat3 was evaluated using Western blot analysis. The nitroglycerin-induced model (NTG) was also employed for the evaluation of migraine.

    RESULTS: FIEO demonstrated significant antinociceptive activity in both phases of the formalin-induced test. However, the FIEO activity was more pronounced effect observed in the second phase. Modulators of the NO-cGMP-K+ channel pathway significantly reversed the antinociceptive activity of FIEO (P 

    MeSH terms: Animals; Disease Models, Animal; Formaldehyde; Male; Nitric Oxide/metabolism; Oils, Volatile/isolation & purification; Oils, Volatile/pharmacology; Pain/drug therapy; Plant Extracts/pharmacology; Plant Extracts/therapeutic use; Rats, Sprague-Dawley*; Plant Roots/chemistry; STAT3 Transcription Factor/metabolism; Rats
  11. Ng WZ, Chan ES, Gourich W, Adiiba SH, Liow MY, Ooi CW, et al.
    Int J Biol Macromol, 2025 Jan;284(Pt 1):138144.
    PMID: 39613062 DOI: 10.1016/j.ijbiomac.2024.138144
    The enzymatic production of fatty acids from vegetable oils is becoming a preferred method due to its mild conditions, simplicity, and scalability. This review analyzes studies on enzymatic hydrolysis, exploring various feedstocks, lipases, reaction conditions, and conversion yields. However, a key limitation is the longer reaction time compared to conventional methods. This limitation is primarily due to the immiscibility of triacylglycerols (TAGs) with water at low temperatures and pressures, as well as the lower activity of enzymes compared to chemical catalysts. To overcome these issues, chemical additives are identified as the most effective process intensification strategy. They are easy to implement, cause less damage to lipases, and are more efficient than mechanical methods. The impact of various chemical additives was thoroughly examined for potential improvements in the enzymatic hydrolysis of vegetable oils. A synergistic combination of chemical additives comprising ionic liquids (ILs) and polyols, along with ultrasound, as well as the consideration of immobilization techniques were explored. Overall, this review highlights the potential of chemical additives and their synergistic feasibility in enhancing the enzymatic performance of lipase-catalyzed hydrolysis reactions.
    MeSH terms: Catalysis; Enzymes, Immobilized/metabolism; Enzymes, Immobilized/chemistry; Hydrolysis; Ionic Liquids/chemistry; Biocatalysis
  12. Nakano H, Alfonso MB, Phinchan N, Jandang S, Manap MRA, Chavanich S, et al.
    Mar Pollut Bull, 2025 Jan;210:117342.
    PMID: 39613519 DOI: 10.1016/j.marpolbul.2024.117342
    Research on microplastics must be harmonized. Therefore, we thoroughly evaluated in the Association of Southeast Asian Nations (ASEAN) region, addressing challenges and priorities in protocol harmonization and microplastics research promotion. Of the 615 papers searched by the Web of Science, 164 were used for this systematic review. The number of ASEAN research articles has increased over time. Examination of research protocols in various sampling environments revealed several challenges: 1) Disparities in access to sampling locations affect the research extent; 2) Outdated protocols and limited access to technologies such as FTIR (Fourier-transform infrared) spectroscopy result in less harmonized and potentially lower-quality data; and 3) Insufficiently detailed methods and QA/QC information hampers comparability. We offer procedure updates to overcome these limitations and cover environmental microplastic study gaps. Other countries in the Global South may encounter similar challenges, making this review a valuable contribution to advancing global microplastics research and fostering international collaboration.
    MeSH terms: Asia, Southeastern
  13. Ceelen DCH, Bracun V, van Essen BJ, Voors AA, de Boer RA, Ter Maaten JM, et al.
    Heart, 2025 Jan 29;111(4):172-179.
    PMID: 39613454 DOI: 10.1136/heartjnl-2024-324486
    BACKGROUND: We evaluated the potential of circulating bone morphogenetic protein 10 (BMP10) as a biomarker for atrial stress and remodelling in patients with heart failure (HF), in comparison to N-terminal pro-B-type natriuretic peptide (NT-proBNP). We also assessed the predictive value of BMP10 for adverse clinical outcomes.

    METHODS: BMP10 levels were quantified in 2085 chronic HF patients from the European BIOlogy Study to TAilored Treatment in Chronic Heart Failure (BIOSTAT-CHF) cohort and in 1487 patients from the Scottish validation cohort. Multivariable linear regression identified independent associates of BMP10. Proteomic analysis of 6369 proteins with subsequent gene set enrichment analysis was used to explore biological pathways associated with elevated BMP10. Cox proportional hazards models adjusting for established risk factors were used to associate BMP10 levels with clinical outcomes, including all-cause mortality and HF hospitalisation.

    RESULTS: In a multivariable model including clinical and echocardiographic parameters, log-transformed and standardised BMP10 levels were significantly associated with a history of atrial fibrillation (Sβ=0.419; p<0.001), and with echocardiographic features reflecting atrial stress, such as increased left atrial diameter (Sβ=0.075; p=0.048). By contrast, these were not among the strongest associates of NT-proBNP levels. Gene set enrichment analysis showed significant overrepresentation in pathways of muscle contraction and extracellular matrix organisation. Higher log-transformed and standardised BMP10 levels predicted a combined outcome of 2-year all-cause mortality and HF rehospitalisation (HR=1.10, 95% CI=1.02-1.19), with the validation cohort yielding comparable results.

    CONCLUSION: BMP10 emerges as a novel biomarker reflecting atrial stress and remodelling in chronic HF patients. Its additional predictive value for adverse outcomes underscores its potential utility in enhancing risk stratification and guiding therapeutic interventions in HF management.

    MeSH terms: Aged; Atrial Fibrillation/blood; Atrial Fibrillation/physiopathology; Echocardiography; Europe/epidemiology; Female; Heart Atria/physiopathology; Humans; Male; Middle Aged; Predictive Value of Tests; Prognosis; Risk Factors; Bone Morphogenetic Proteins; Proteomics/methods
  14. Hufana-Duran D, Chaikhun-Marcou T, Duran PG, Atabay EP, Nguyen HT, Atabay EC, et al.
    Theriogenology, 2025 Feb;233:123-130.
    PMID: 39613496 DOI: 10.1016/j.theriogenology.2024.11.016
    The future of reproductive biotechnologies in water buffalo in Southeast Asian countries holds significant promise for enhancing genetic quality and productivity. Fixed-time artificial insemination remains the commonly used technology, with advances in assisted reproductive technologies (ART) such as in vitro embryo production (IVEP), embryo transfer (ET), and the use of sex-sorted sperm increasingly adopted to improve breeding efficiency. These technologies overcome traditional breeding limitations, such as low reproductive rates, genetic diversity constraints, and the production of sex-predetermined offspring. The application of multiple ovulation and embryo transfer (MOET) is constrained by poor embryo recovery in this livestock species. Somatic cell nuclear transfer (SCNT) offers great potential for producing sex-predetermined and genetically superior buffalo but requires further research to increase efficiency. Cryopreservation of buffalo genetics is bolstered by the establishment of Gene Banks. Challenges such as high costs, the need for skilled personnel, and infrastructure development remain constraints. Integration of genomic selection, automation, and expansion of ET programs are clear directions. Strengthening research and collaboration among Southeast Asian countries is essential to fully realize the benefits of these biotechnologies and ensure sustainable and profitable buffalo farming.
    MeSH terms: Animals; Asia, Southeastern; Biotechnology; Female; Insemination, Artificial/veterinary; Male; Cryopreservation/veterinary
  15. Cheow PS, Tan TK, Song AA, Yusoff K, Chia SL
    Avian Pathol, 2025 Feb;54(1):149-157.
    PMID: 39318350 DOI: 10.1080/03079457.2024.2403412
    Development of nr-NDV.Reverse transfection was applied for the recovery of nr-NDV.Propagation of nr-NDV was done by sub-passaging transfected BSR T7/5 cells.Safety profile was done to prove that the nr-NDV is non-replicating.
    MeSH terms: Animals; Cell Line; Chickens/virology; Recombination, Genetic; Transfection/veterinary; Virus Replication
  16. Ratnasingam J, Lim QH, Chan SP
    Curr Opin Endocrinol Diabetes Obes, 2025 Feb 01;32(1):20-25.
    PMID: 39607025 DOI: 10.1097/MED.0000000000000895
    PURPOSE OF REVIEW: The aim of this article was to review the up-to-date evidence with regards to the unique features of the Type 2 diabetes (T2D) pathophysiology, complications, response to therapy with the possibility of precision medicine guiding therapeutic decision making in Asia.

    RECENT FINDINGS: Asia is the epicenter of diabetes. There have been marked advances with genotyping and phenotyping of the Asian patient with T2D, particularly with young onset diabetes where early beta cell failure and rapid progression of complications are more frequent. As Asians have lower muscle mass and higher adiposity, sarcopenia is increasingly associated with diabetes. Response to lifestyle and pharmacotherapy are generally similar, but unique features exist with different populations. Across Asia, use of guideline directed medical therapy for cardio-renal protection are recommended, but uptake of these newer agents are suboptimal and barriers exist with regards to standardized care.

    SUMMARY: Although many similarities have been observed across Asia, due to the heterogeneity of populations within Asia, further research is required to streamline and pave the way towards precision medicine. There is an urgent need for region wide consensus to minimize barriers to diabetes care and stigma in diabetes terminology across Asia.

    MeSH terms: Asia/epidemiology; Humans; Hypoglycemic Agents/therapeutic use; Asian Continental Ancestry Group*; Precision Medicine
  17. Nordin NA, Soon S, Senawi JB, Jinin ZAM, Arshad SS, Yasmin AR, et al.
    Appl Biochem Biotechnol, 2025 Mar;197(3):1760-1772.
    PMID: 39607468 DOI: 10.1007/s12010-024-05093-0
    Foot-and-mouth disease (FMD) is known for its highly contagious properties among cloven-hoofed animals resulting in significant morbidity rates. Incursions of this disease have caused significant losses in affected countries in Southeast Asia and Africa, even within EU countries which resulted in significant financial losses. This study is aimed at addressing existing limitations by creating a diagnostic method using aptamer-based assay. Three DNA aptamers were engineered to target the VP2 region of the FMD viral capsid protein. Since VP2 demonstrates a highly conserved amino acid sequence across serotypes, the specifically designed aptamers can detect different serotypes of the virus. Aptamers were evaluated against VP2 capsid protein, which was synthesized based on sequences from serotypes A, O, and Asia 1 of the FMD virus. After the recombinant VP2 capsid protein was developed, expressed, and refined, it was applied using enzyme-linked aptamer sorbent assay (ELASA) to determine aptamers' binding capability. A similar test was further conducted with purified FMD virus from serotype A and serotype O. The ELASA results displayed a notable sensitivity in identifying the FMDV. Under optimized conditions, the aptamers have LOD as low as 0.11 ng/mL with LOQ as low as 0.34 ng/mL. The binding strength analyzed using the equilibrium dissociation constant (Kd) showed strong binding affinity at 3.092 ± 0.05 nM. Based on these findings, the method shows significant potential with high sensitivity and specificity for FMD virus detection assay.
    MeSH terms: Animals; DNA, Single-Stranded/chemistry; Foot-and-Mouth Disease/diagnosis; Foot-and-Mouth Disease/virology; DNA Probes/genetics; DNA Probes/chemistry
  18. Karandagaspitiya CO, Mahendra CK, Lim HP, Chan DK, Tey YS, Kam CF, et al.
    Food Chem, 2025 Feb 28;466:142228.
    PMID: 39608119 DOI: 10.1016/j.foodchem.2024.142228
    This research explores the interactions of tripolyphosphate-chitosan-pea protein (TPP-CS-PP) in improving the stability and storage of 3D printing food inks. Chitosan (CS) and pea protein (PP) were complexed at various concentrations with 80 % palm olein to produce high internal phase Pickering emulsions (HIPPEs) 3D printing food inks. The resulting CSPP HIPPEs exhibited shear-thinning behaviour and the flexibility to switch between solid and liquid states, ideal for 3D printing. CSPP1:150 achieved the best 3D printing resolution and shape fidelity due to electrostatic attraction of CS-PP and excess PP enhancing adhesion at the oil/water interface. After spraying tripolyphosphate (TPP), crosslinking with CS and phosphorylation of PP further improved HIPPE resistance to deformation and oiling off for 2 days post-printing. This is a significant improvement over the control. Thus, further investigation on the interaction of TPP with CS and PP is warranted to further improve the storage stability of 3D printed food inks.
    MeSH terms: Polyphosphates/chemistry; Food Packaging/instrumentation; Printing, Three-Dimensional*
  19. Bhat AA, Altamimi ASA, Goyal A, Goyal K, Kaur I, Kumar S, et al.
    Int Immunopharmacol, 2025 Jan 10;144:113675.
    PMID: 39608172 DOI: 10.1016/j.intimp.2024.113675
    CAR T cell therapy has revolutionized how we deliver cancer treatment, most notably for hematologic cancers, by compelling T cells to recognize and kill tumor cells. Nevertheless, current obstacles to utilizing this therapy in solid tumors and overcoming cancer resistance include radicalization. This review discusses how CD95 modulation can boost CAR T cell efficacy. Traditionally, CD95 was known to execute apoptosis induction, but it plays a dual role in induced cell death or in supporting cancer cell survival. Recent data have demonstrated that cancer cells escape CD95-mediated apoptosis via the downregulation of CD95, caspase 8 mutation, or the expression of the inhibition protein cFLIP. Additionally, the immunosuppressive tumor microenvironment, containing CD95L expressing immune cells, explains CAR T cell therapy resistance. Furthermore, we characterize the therapeutic potential of CD95 targeted approaches, including CD95L inhibition (APG101) and alterations in CAR T cell manufacturing (tyrosine kinase inhibitors to mitigate fratricide). In this review, we highlight the importance of multi-path way strategies combining CD95 modulation with CAR T cell engineering to overcome resistance, specifically to target tumor cells better and sustain CAR T cell persistence to enhance treatment efficacy in solid tumors.
    MeSH terms: Animals; Humans; T-Lymphocytes/immunology; Apoptosis
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