Browse publications by year: 2025

  1. Chauhan K, Royse A, Goh I, Crozier I, Wynn G
    J Cardiovasc Electrophysiol, 2025 Jan;36(1):295-297.
    PMID: 39575491 DOI: 10.1111/jce.16511
    INTRODUCTION: Substernal extravascular defibrillators (EV ICDs) have been shown to be effective and safe for patients at risk of sudden cardiac death, however, there is little evidence around the safety of extracting chronic devices.

    METHODS AND RESULTS: We present a 50-year-old patient in whom a Medtronic EV ICD system was successfully removed without specialist extraction tools, 186 weeks after implantation, by an operator experienced in transvenous lead extraction but without formal training in EVICD implantation.

    CONCLUSION: The successful extraction of an EV ICD system is possible without specialised tools at least 3.6 years post-implant.

    MeSH terms: Humans; Male; Middle Aged; Prosthesis Design; Time Factors; Treatment Outcome; Defibrillators, Implantable*; Device Removal*
  2. Rajandram R, Suren Raj TL, Gobe GC, Kuppusamy S
    Clin Chim Acta, 2025 Jan 15;565:119964.
    PMID: 39265757 DOI: 10.1016/j.cca.2024.119964
    Liquid biopsies offer a less invasive alternative to tissue biopsies for diagnosis, prognosis, and determining therapeutic potential in renal cell carcinoma (RCC). Unfortunately, clinical studies using liquid biopsy biomarkers in RCC are limited. Accordingly, we examine RCC biomarkers, derived from urine, plasma, serum and feces of potential impact and clinical outcome in these patients. A PRISMA checklist was used to identify valuable liquid biopsy biomarkers for diagnosis (plasma cfDNA, serum- or urine-derived circulating RNAs, exosomes and proteins), prognosis (plasma cfDNA, plasma- or serum-derived RNAs, and proteins), and therapeutic response (plasma- and serum-derived proteins). Although other analytes have been identified, their application for routine clinical use remains unclear. In general, panels appear more effective than single biomarkers. Important considerations included proof of reproducibility. Unfortunately, many of the examined studies were insufficiently large and lacked multi-center rigor. Cost-effectiveness was also not available. Accordingly, it is clear that more standardized protocols need to be developed before liquid biopsies can be successfully integrated into clinical practice in RCC.
    MeSH terms: Humans
  3. Bao L, Yang R, Diao D, Tian F, Chen Y, Zheng B, et al.
    Food Chem, 2025 Feb 01;464(Pt 1):141466.
    PMID: 39406135 DOI: 10.1016/j.foodchem.2024.141466
    This study investigated the effect of different reheating treatments on gel properties and flavor changes of surimi products. As the reheating temperature increased from 90 °C to 121 °C, the heat-induced proteolysis produced more abundant umami and sweet amino acids, which took part in the conversion of IMP to AMP, thus enhancing the taste profiles. Reheating increased the exposure of active -NH2 terminals in proteins, which boosted Maillard and Strecker reactions with carbonyl compounds originated from fatty acid oxidation, thus not only reducing the aldehydes and esters contents but also lowering the whiteness of surimi products. Reheating at 90 °C prohibited the production of warmed-over flavor (WOF) and well-preserved the textural characteristics, but high temperatures ≥100 °C were prone to generate furan as the major WOF substance and to destroy gel structures. Collectively, this study provides new insights on understanding the role of reheating on sensory properties of surimi products.
    MeSH terms: Amino Acids/analysis; Amino Acids/chemistry; Animals; Cooking; Flavoring Agents/chemistry; Hot Temperature; Humans; Taste*; Maillard Reaction; Seafood/analysis
  4. Liu Z, Lee YY, Tan CP, Wang Y, Qiu C
    Food Chem, 2025 Feb 01;464(Pt 1):141553.
    PMID: 39406140 DOI: 10.1016/j.foodchem.2024.141553
    Cyclolinopeptides (CLs) have anti-inflammatory, anti-osteoporosis, and anti-tumor effects, however, low water and oil solubility greatly limit their application. Herein, CLs-loaded β-cyclodextrin (β-CD) emulsions were prepared with different oil phases. The in vitro digestibility, cellular absorption, and anti-inflammatory effects were evaluated. Camellia oil diacylglycerol (CO DAG) showed enhanced dissolving ability for CLs due to high polarity. β-CD formed inclusion complexes with DAG through hydrogen bond and the emulsions showed smaller size and higher physical stability with 50 % (w/w) oil. The in vitro digestibility of the DAG emulsion was increased and the CLs' bioavailability was 13.6-fold higher than CLs in oil. The CLs-loaded Pickering emulsion digesta exhibited a higher nitric oxides (NO) inhibition rate (58.62 %) and Caco-2 cell penetration (3.09 × 10-6 cm/s). Therefore, emulsion formulated with β-cyclodextrin and DAG can effectively improve the solubility and bioavailability of CLs, which has significant potential for application in functional foods and pharmaceutical industry.
    MeSH terms: Animals; Anti-Inflammatory Agents/pharmacology; Anti-Inflammatory Agents/chemistry; Biological Availability*; Humans; Particle Size; Solubility*; Caco-2 Cells
  5. Kuan WC, Lim KK, Chee KH, Kasim S, Dujaili JA, Lee KK, et al.
    Qual Life Res, 2025 Feb;34(2):471-484.
    PMID: 39476200 DOI: 10.1007/s11136-024-03818-6
    PURPOSE: This study aimed to examine the trajectory in health-related quality of life (HRQoL) during and after hospitalisation for worsening of heart failure (HF) in Malaysia.

    METHODS: 200 patients with heart failure and reduced ejection fraction (HFrEF) admitted into two hospitals in Malaysia due to worsening of HF were surveyed using the EQ-5D-5 L questionnaire. The primary outcomes were utility values at admission, discharge and 1-month post-discharge (1MPD). Secondary outcomes included the visual analogue scores (VAS) and the proportion of patients reporting each EQ-5D-5 L dimension levels. Missing data were imputed using multiple imputation, and generalised linear mixed models were fitted.

    RESULTS: At admission, the unadjusted mean utility values and VAS scores for HFrEF patients in Malaysia were as low as 0.150 ± 0.393 and 38.2 ± 20.8, respectively. After a median hospital stay of 4 days, there was a significant improvement in utility values and VAS scores by 0.510 (95% CI: 0.455-0.564) and 28.8 (95% CI: 25.5-32.1), respectively. The utility value and VAS score at 1-month post-discharge were not significantly different from discharge. The proportion of HFrEF patients reporting problems and severe problems in mobility, self-care, usual activities, and anxiety/depression, pain/discomfort reduced at varying degree from admission to discharge and 1MPD.

    CONCLUSION: HF is a progressive condition with substantial variation in HRQoL during the disease trajectory. During hospitalisation due to worsening of HF, HFrEF population has unfavourable HRQoL. Rapid and significant HRQoL improvement was observed at discharge, which sustained over one month. The study findings can inform future cost-effectiveness analyses and policies.

    MeSH terms: Aged; Female; Humans; Malaysia; Male; Middle Aged; Quality of Life*; Surveys and Questionnaires; Disease Progression
  6. Turkyilmaz I, Abdullah JY, Wilkins GN, Bernardi S, Varvara G
    Minerva Dent Oral Sci, 2025 Feb;74(1):12-19.
    PMID: 39565339 DOI: 10.23736/S2724-6329.24.04898-8
    BACKGROUND: The purpose of this study was to determine the accuracy of an intraoral scanner to digitally duplicate complete dentures and analyze the effects of mesh reduction of digital files on the surface area and volume accuracy of complete dentures, in vitro.

    METHODS: A mandibular complete denture was scanned by a desktop scanner to create a digital STL reference file (control). Fifteen identical scans were created by using an intraoral scanner and exported as STL files (test group). These 15 files were saved at 100% of the original scan resolution then reduced to 75%, 50%, 25%, and 10% of their original quality. These 75 scans were statistically analyzed by calculating The Hausdorff Distance (HD) and Dice Similarity Coefficients (DSC) to assess the variation between the mean reduced intraoral scanner files test and the control desktop scanner file and eventual inconsistencies. The volumes of the reduced mesh files were also compared with the 100% resolution intraoral mesh files to evaluate precision and trueness of the intraoral scanner.

    RESULTS: Reduced mesh files of 10%, 25%, 50%, 75% of the original scan yielded a percentage similarity average of 99.7%, indicating a very high precision value for the intraoral scanner. Also, the volumes of each associated mesh reduction slightly decreased with non-statistically significant results.

    CONCLUSIONS: This study concluded that the chosen intraoral scanner for this study provided very high trueness (98.34%) and precision (99.7%), and also the volumes of reduced mesh files slightly decreased but were not statistically significant.

    MeSH terms: Dental Impression Technique/instrumentation; Denture, Complete*; Humans; Computer-Aided Design
  7. Gao F, Chui PL, Che CC, Wang K
    Geriatr Nurs, 2025;61:192-199.
    PMID: 39566231 DOI: 10.1016/j.gerinurse.2024.10.061
    OBJECTIVE: To determine the mediating role of family cohesion in the relationship between death anxiety and readiness toward advance care planning (ACP).

    METHODS: This study included 965 Chinese community-dwelling older adults. Pearson correlation coefficient was conducted to assess the relationship between readiness toward ACP, death anxiety, and family cohesion. Structural equation model was used to examine the study hypothetical model.

    RESULTS: 965 valid questionnaires were collected. Death anxiety is significantly related to the readiness toward ACP (r = -0.437, P < 0.01) and family cohesion (r = -0.444, P < 0.01), and family cohesion exhibited a positive correlation with readiness toward ACP (r = 0.499, P < 0.01). Family cohesion partially mediated the effect of death anxiety on readiness toward ACP, accounting for 35.94 % of the total effect.

    CONCLUSIONS: Family cohesion mediates the relationship between death anxiety and readiness toward ACP. Healthcare professionals should implement measures to alleviate death anxiety and promote family cohesion in older adults, thereby enhancing their readiness toward ACP.

    MeSH terms: Aged; Aged, 80 and over; Attitude to Death*; China; Cross-Sectional Studies; Family/psychology; Family Relations/psychology; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Advance Care Planning*
  8. Waheed A, Akram S, Butt FW, Liaqat Z, Siddique M, Anwar F, et al.
    J Chromatogr A, 2025 Jan 04;1739:465503.
    PMID: 39566285 DOI: 10.1016/j.chroma.2024.465503
    Ionic liquids (ILs) have emerged as more desirable liquids than conventional solvents for chemistry, material science, engineering and environmental science. The scientific literature reveals an exponential increase in the number of research projects aimed at exploring the chromatographic features of ionic liquids. The review provides sound scientific data to examine the structural characteristics of ionic liquids that make them ideal for use in chromatography. This contribution is distinctive since it integrates the synthesis, benefits, drawbacks, and possible uses of ionic liquids in several chromatographic separation processes. Keeping the cation the same, the introduction of different anions is also possible, and this strategy leads to the synthesis of a series of different ionic liquids with varying properties. A detailed probe is given on the influence of ionic liquid structure and properties on their chromatographic behavior, both as stationary phase and mobile phase and/or mobile phase additives. Ionic liquid based immobilized stationary phases and their analyte retention mechanisms (hydrogen bonding, electrostatic forces of attraction, π-π stacking, ion exchange, and hydrophilic interactions, etc.) are critically discussed. Finally, a thorough analysis of the literature suggests that IL-based stationary phases may undergo multi-mode and more flexible retention mechanisms. Their dual polarity can facilitate interaction with both polar and non-polar compounds. Similarly, using IL as a mobile phase can offer more pragmatic and sustainable options for enantiomer separation.
    MeSH terms: Hydrogen Bonding; Stereoisomerism; Hydrophobic and Hydrophilic Interactions
  9. Mohamed Thaha UAB, Wan Mohamad WM, Nik Husain NR, Yusop N, Mohamud R, Wan Ghazali WS
    Int Immunopharmacol, 2025 Jan 10;144:113597.
    PMID: 39566387 DOI: 10.1016/j.intimp.2024.113597
    BACKGROUND: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by dysregulated immune responses and inflammation. Interleukin-37 (IL-37) is a recently discovered immunomodulatory cytokine with potential anti-inflammatory properties. This systematic review explores the relationship between IL and 37 and SLE disease activity, and evaluates its potential as a therapeutic agent.

    METHODS: Electronic databases were searched for studies investigating IL-37 and SLE. Data on IL-37 levels, SLE Disease Activity Index (SLEDAI) score, genetic polymorphisms, and its therapeutic effects from pre-clinical studies were extracted.

    RESULTS: Previous studies presented conflicting findings on IL-37 levels in SLE patients. Some reported positive correlations with disease activity, while others observed associations between lower IL-37 and increased activity. Genetic variations in the IL-37 gene linked to SLE susceptibility have been reported. Pre-clinical studies using engineered mesenchymal stem cells or direct IL-37 treatment showed promise in reducing disease severity in mouse models and cell cultures of SLE. The analysis of multiple studies reveals that IL-37 expression varies significantly across different SLE subtypes.

    CONCLUSIONS: While a potential link exists between IL and 37 and disease activity, genetic predisposition, and therapeutic benefit, further research is needed. Future studies with standardized designs, larger and more diverse populations, and mechanistic investigations are crucial to determine the therapeutic potential of IL-37 for SLE. This review highlights the need for well-designed clinical trials to evaluate the safety and efficacy of IL-37 therapy in patients with SLE.

    MeSH terms: Animals; Humans; Genetic Predisposition to Disease; Mice
  10. Lee ZM, Goh BH, Khaw KY
    Planta Med, 2025 Jan;91(1-02):4-18.
    PMID: 39566518 DOI: 10.1055/a-2446-2146
    Aloe vera is commonly used as traditional medicine for cutaneous wound healing. Nonetheless, the wound healing mechanisms of Aloe vera remain unclear. This review aims to provide insight into the molecular mechanisms of Aloe vera in promoting cutaneous wound healing, with particular emphasis on the mechanisms that stimulate cell proliferation and migration. Aloe vera has been shown to upregulate growth factors such as keratinocyte growth factor-1 (KGF-1), transforming growth factor-β (TGF-β), cyclin D1, insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), and microfibril-associated glycoprotein 4 (MFAP4), as well as collagen, fibrillin, elastin, α-smooth muscle actin (α-SMA), integrins, and platelet endothelial cell adhesion molecule 1 (PECAM-1, also known as CD31), while downregulating the expression of matrix metalloproteinases (MMPs). In addition, Aloe vera was also found to upregulate PI3K/Akt and MAPK pathways, as well as the TGF-β signalling pathway via Smad proteins. Furthermore, molecular docking studies revealed that certain chemical constituents of Aloe vera bind to some of the forementioned growth factors or signalling molecules. With regards to current applications, although human clinical trials have reported positive results from using Aloe vera in healing open wounds and burns and alleviating some inflammatory skin diseases, the current commercial uses of Aloe vera remain largely focused on cosmetic products. Thus, greater advances are required to promote the use of Aloe vera products in clinical settings.
    MeSH terms: Animals; Humans; Plant Extracts/pharmacology; Plant Extracts/chemistry; Skin/drug effects; Signal Transduction/drug effects; Cell Proliferation/drug effects
  11. Goh SM, Dassanayake MK, Foan CC, Wiart C, Symonds R, Khoo TJ, et al.
    Microb Pathog, 2025 Jan;198:107134.
    PMID: 39566830 DOI: 10.1016/j.micpath.2024.107134
    BACKGROUND AND OBJECTIVES: Plants contain a wide variety of bioactive compounds, which have attracted the interest of researchers in finding novel sources of natural medicine. In the following paper, we aim to evaluate the antibacterial potential of extract fractions associated with Parkia speciosa pods and beans against human pathogenic bacteria.

    METHODS: Antimicrobial activity was determined with disc diffusion and broth microdilution assays against eight skin colonising microorganisms including Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia followed by further fractionation of the pods ethyl acetate fraction by column chromatography along with preparative thin-layer chromatography. Quantification of bacterial death mechanism was elucidated by the measurement of hole size in cell wall that has been induced by extract constituents via field-emission scanning electron microscopy (FESEM).

    RESULTS: Four fractions showed significant antimicrobial activity against the six microorganisms tested (p 

    MeSH terms: Escherichia coli/drug effects; Humans; Klebsiella pneumoniae/drug effects; Malaysia; Microbial Sensitivity Tests*; Pseudomonas aeruginosa/drug effects; Staphylococcus aureus/drug effects; Salmonella enterica/drug effects
  12. Beam M, Abdull Wahab SF, Ramos M
    Med Clin North Am, 2025 Jan;109(1):313-324.
    PMID: 39567101 DOI: 10.1016/j.mcna.2024.06.005
    Point-of-care ultrasound (POCUS) bridges diagnostic gaps across the continuum of care worldwide and is a particularly potent tool in resource-limited settings (RLS). To capture the scope of its current impact in RLS, this narrative review of POCUS use in public health, primary care, outpatient specialty, pre-hospital, and palliative care settings discusses its use in reducing diagnostic health care inequities. Disease-specific protocols, longitudinal training, quality assurance, and task shifting are key to robust expansion of POCUS in RLS.
    MeSH terms: Developing Countries; Health Resources; Humans; Primary Health Care; Point-of-Care Systems*
  13. Poultsides L, Achan P, Al-Dosari MMA, Al Maskari S, Abbas AA, Bahairy WS, et al.
    J Arthroplasty, 2025 Feb;40(2S1):S1-S4.
    PMID: 39447927 DOI: 10.1016/j.arth.2024.10.091
  14. Al-Thiabat MG, Agrawal M, Kumar Sahu K, Alhawarri MB, Banisalman K, Al Jabal GA, et al.
    Chem Biodivers, 2025 Mar;22(3):e202402351.
    PMID: 39471253 DOI: 10.1002/cbdv.202402351
    This study explores the therapeutic potential of three proaporphine alkaloids-cissamaline, cissamanine, and cissamdine, which were recently isolated from Cissampelos capensis L.f., against Parkinson's disease (PD). Using computational techniques, we investigated their efficacy as inhibitors of a key protein in PD. ADMET analysis demonstrated that these alkaloids conform to the Lipinski, Pfizer, Golden Triangle, and GSK rules, indicating favorable safety, oral bioavailability, and a high probability of passing the human intestinal and blood-brain barriers. They were neither substrates nor inhibitors of any CYP enzymes tested, indicating minimal metabolic interference and an enhanced safety profile. Molecular docking studies revealed binding energies of -9.05 kcal/mol (cissamaline), -9.95 kcal/mol (cissamanine), and -10.65 kcal/mol (cissamdine) against MAO-B, a critical PD target, surpassing the control (zonisamide, -6.96 kcal/mol). The molecular interaction analyses were also promising, with interactions comparable to the control. Molecular dynamics (MD) simulations confirmed stable protein-ligand interactions, with root-mean-square deviation (RMSD) values ranging from 1.03 Å to 3.92 Å, root-mean-square fluctuation (RMSF) values remaining below 1.14 Å, and radius of gyration (RGyr) values between 20.20 Å and 20.50 Å, indicating compact structures. Hydrogen bonding analysis revealed maximum hydrogen bond counts of 6 (cissamanine), 5 (cissamaline), and 4 (cissamdine), demonstrating robust interactions with MAO-B. Density Functional Theory (DFT) calculations revealed the highest electrophilicity (ω =0.151), highest electron affinity (EA =0.075), and smallest HOMO-LUMO gap (ΔE =0.130) for cissamanine, indicating enhanced reactivity. These results advocate for further in vitro and in vivo studies to evaluate the compounds' potential as PD therapeutics.
    MeSH terms: Humans; Molecular Structure; Molecular Dynamics Simulation*; Molecular Docking Simulation*
  15. Wang Y, Guo M, Vo Thanh H, Zhang H, Liu X, Zheng Q, et al.
    J Adv Res, 2025 Apr;70:587-601.
    PMID: 39521430 DOI: 10.1016/j.jare.2024.10.034
    INTRODUCTION: Underground coal fires pose significant environmental and health risks due to releasing CO2 emissions. Predicting surface CO2 flux accurately in underground coal fire areas is crucial for understanding the distribution of spontaneous combustion zones and developing effective mitigation strategies. In recent years, advanced machine learning techniques have shown promise in various carbon-related studies. This research uses an experimental approach to explore the power of advanced machine learning schemes for predicting CO2 flux in underground coal fire areas.

    OBJECTIVES: By leveraging the power of advanced machine learning schemes and experimental approaches, this research aims to provide valuable insights into CO2 flux prediction in coal fire areas and inform environmental monitoring and management strategies.

    METHODS: The study involves the collection of an experimental dataset specific to underground coal fire areas, encompassing various parameters related to CO2 flux and underground coal fire characteristics. Innovative feature engineering techniques are applied to capture the unique characteristics of underground coal fire areas and their impact on CO2 flux. Different machine learning algorithms, including Natural gradient boosting regression (NGRB), Extreme gradient boosting (XGboost), Light gradient boosting (LGRB), and random forest (RF), are evaluated and compared for their predictive capabilities. The models are trained, optimized, and assessed using appropriate performance metrics.

    RESULTS: The NGRB model yields the best predictive performances with R2 of 0.967 and MAE of 0.234. The novel contributions of this study include the development of accurate prediction models tailored to underground coal fire areas, shedding light on the underlying factors driving CO2 flux. The findings have practical implications for delineating the spontaneous combustion zone and mitigating CO2 emissions from underground coal fires, contributing to global efforts in combating climate change.

  16. Yucekul A, Yilgor C, Demirci N, Gurel IE, Orhun O, Karaman MI, et al.
    Eur Spine J, 2025 Jan;34(1):251-262.
    PMID: 39560722 DOI: 10.1007/s00586-024-08488-z
    PURPOSE: Appendicular skeleton markers are commonly used for maturity assessment for Adolescent Idiopathic Scoliosis (AIS) patients. Traditionally, Risser has been a standard skeletal maturity assessment method. More recently, Sanders classification (SSMS), as a more comprehensive system, became popular, especially in decision-making for Vertebral Body Tethering (VBT). Thumb-Ossification Composite Index (TOCI), using ossification of thumb epiphyses, has been claimed to more accurately stage patients around their peak height velocity. However, growth peaks may occur separately at lower limbs and trunk. Hence, Cervical Vertebral Maturity (CVM), using cervical spine morphology, possesses a potential to better estimate spinal growth as it uses axial skeleton markers instead of appendicular skeleton markers. The aim of the study was to compare various axial and appendicular skeletal maturity assessment methods for longitudinal growth and curve modulation after VBT.

    METHODS: A retrospective analysis of prospectively collected data was conducted. Skeletal maturity was determined using Risser, SSMS, TOCI and CVM for each patient. Crosstabulations of axial vs. appendicular markers were formed to analyze their concordance and discordance. Logistic and logarithmic regression models were run to assess longitudinal growth (postoperative height gain and leg-length growth) and curve modulation (follow-up instrumented Cobb correction after index operation), respectively. Models were compared using Akaike information criterion (AIC).

    RESULTS: 34 patients (32 F/2 M, mean age: 12.8 ± 1.5 years, mean follow-up: 47.7 (24-80) months) were included. The median preoperative maturity stages were: Risser: 1 (-1-4), SSMS: 4 (1-7), TOCI: 6 (1-8) and CVM: 4 (1-6). At latest follow-up, all patients reached skeletal maturity. Concordance and discordance were observed between axial vs. appendicular systems that demonstrated a range of possible distributions of CVM, where trunk peak height velocity occurred before, simultaneously with or after the standing height peak height velocity. R-squared values for Risser, SSMS, TOCI and CVM were 0.701, 0.783, 0.810 and 0.811, respectively, for prediction of final height; 0.759, 0.821, 0.831 and 0.775 for final leg-length, and 0.507, 0.588, 0.668 and 0.673 for curve modulation. Delta AIC values demonstrated that different skeletal maturity assessment methods provided distinctive information regarding follow-up height gain, leg-length growth and curve behavior.

    CONCLUSIONS: Risser score provided considerably less information for all three outcome variables. TOCI and SSMS provided substantial information regarding remaining leg-length assessments, while in terms of assessment of total height gain and curve modulation after surgery, CVM and TOCI offered substantial information and SSMS offered strong information. Mutual use of axial and appendicular markers may provide valuable insight concerning timing of surgery and magnitude of surgical correction.

    MeSH terms: Adolescent; Age Determination by Skeleton/methods; Cervical Vertebrae/growth & development; Cervical Vertebrae/surgery; Child; Female; Humans; Male; Retrospective Studies
  17. Palanisamy J, Palanichamy VS, Vellaichamy G, Perumal P, Vinayagam J, Gunalan S, et al.
    Naunyn Schmiedebergs Arch Pharmacol, 2025 Apr;398(4):3409-3432.
    PMID: 39560753 DOI: 10.1007/s00210-024-03547-0
    The primary purpose of this review is to explore the green synthesis of silver nanoparticle (AgNP) using natural biomolecules derived from marine sources. This review aims to evaluate the effectiveness of environmentally friendly approaches for synthesizing AgNPs and to examine their potential applications across various fields such as medicine, biotechnology, and environmental remediation. The key research question focuses on understanding how marine biomolecules, including polysaccharides, proteins, enzymes, amino acids, alkaloids, and vitamins, contribute to the formation of AgNPs and how these green-synthesized nanoparticles retain their functional properties. This review systematically examines current literature on the green synthesis of AgNPs, focusing on marine-derived biomolecules such as polysaccharides, proteins, and alkaloids. The methodology includes analyzing green synthesis techniques and comparing them with traditional chemical methods to highlight environmental benefits and overall efficiency. Various marine species, such as seagrass and seaweed, are explored as potent agents in the reduction of silver ions. The findings reveal that green synthesis of AgNPs using marine biomolecules is not only environmentally sustainable but also retains the desirable properties of the nanoparticles, such as antimicrobial, antioxidant, and anticancer activities. Additionally, the green-synthesized AgNPs show significant potential applications in mosquito control, wound healing, and anticancer therapies. Green synthesis of AgNPs using marine sources presents a viable and sustainable alternative to conventional chemical methods, significantly reducing the environmental impact of nanoparticle production while ensuring biocompatibility and functional integrity. This approach holds promise for diverse applications in biomedicine, environmental remediation, and beyond. Further research is recommended to address challenges in scaling up production and commercialization.
    MeSH terms: Animals; Humans; Aquatic Organisms*
  18. Prazeres F, Jamoulle M, Kareli A, Collins C, Móczár C, Wong MCS, et al.
    Fam Pract, 2025 Feb 07;42(2).
    PMID: 39561247 DOI: 10.1093/fampra/cmae061
    INTRODUCTION: The evolving landscape of general practice (GP)/family medicine (FM) in the post-COVID-19 era, focussing on integrating telemedicine and remote consultations requires a new definition for this specialty. Hence, a broader consensus-based definition of post-COVID-19 GP/FM is warranted.

    METHODS: This study involved a modified electronic Delphi technique involving 27 specialists working in primary care recruited via convenient and snowball sampling. The Delphi survey was conducted online between August 2022 and April 2023, utilizing the Google Forms platform. Descriptive statistics were employed to analyse consensus across Delphi rounds.

    RESULTS: Twenty-six international experts participated in the survey. The retention rate through the second and third Delphi rounds was 96.2% (n = 25). The broader consensus definition emphasizes person-centred care, collaborative patient-physician partnerships, and a holistic approach to health, including managing acute and chronic conditions through in-person or remote access based on patient preferences, medical needs, and local health system organization.

    CONCLUSION: The study highlights the importance of continuity of care, prevention, and coordination with other healthcare professionals as core values of primary care. It also reflects the role of GP/FM in addressing new challenges post-pandemic, such as healthcare delivery beyond standard face-to-face care (e.g. remote consultations) and an increasingly important role in the prevention of infectious diseases. This underscores the need for ongoing research and patient involvement to continually refine and improve primary healthcare delivery in response to changing healthcare landscapes.

    MeSH terms: Delphi Technique*; Family Practice*; Female; Humans; Male; Primary Health Care; Telemedicine*; Patient-Centered Care; Remote Consultation; Consensus
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