Browse publications by year: 2025

  1. Yeo YH, San BJ, Ahmad E, Tan MC, Sin YM, Jani M, et al.
    Prev Med, 2025 Jan;190:108179.
    PMID: 39561854 DOI: 10.1016/j.ypmed.2024.108179
    OBJECTIVE: Our study aimed to assess the heart failure/cardiomyopathy-related population-level mortality trends among patients with obesity in the United States and disparities across demographics.

    METHODS: We queried the Centers for Disease Control and Prevention's Wide-Ranging Online Data for Epidemiologic Research database among adults aged ≥25 from 1999 to 2019. Heart failure/cardiomyopathy were listed as the main causes of death, with obesity as a contributing cause. We calculated age-adjusted mortality rates (AAMR) per 100,000 individuals and estimated the average annual percent change (AAPC). We also evaluated the social vulnerability of United States counties (2014-2018).

    RESULTS: There were 29,334 deaths related to heart failure/cardiomyopathy among patients with comorbid obesity. The overall AAMR increased from 0.41 in 1999 to 0.94 in 2019, with an AAPC of 3.78 (95 % CI, 3.41-4.14). The crude mortality rate increase for heart failure/cardiomyopathy was greater in individuals with comorbid obesity than in those without. Males had a higher AAMR than females (0.78 vs 0.55). African Americans also had higher AAMR than Whites (1.35 vs 0.62). The AAMR was higher in rural areas than in urban regions (0.76 vs 0.66). The overall AAMR was higher in counties with social vulnerability index-Quartile 4 (SVI-Q4) (most vulnerable) (1.08) compared to SVI-Q1 (least vulnerable) (0.63) with a risk ratio of 1.71 (95 % CI: 1.61-1.83).

    CONCLUSION: Heart failure/cardiomyopathy mortality in individuals with comorbid obesity was rising. Males, African Americans, and individuals from rural regions had higher AAMR than their counterparts.

    MeSH terms: Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; United States/epidemiology; Comorbidity; Health Status Disparities
  2. Bhardwaj BY, Vihal S, Pahwa R, Agarwal S, Gupta B, Yang JC, et al.
    Carbohydr Polym, 2025 Jan 15;348(Pt A):122809.
    PMID: 39562084 DOI: 10.1016/j.carbpol.2024.122809
    Non-toxic, biocompatible, biodegradable, and bioadhesive, characteristics, of natural polysaccharides, are widely recognized and well accepted. Their usage in dietary, medicinal, biomedical, and cosmetic, applications is due to their unique and fascinating attributes. Xanthan gum, a microbial polysaccharide possesses diverse-wonderful features. It is a naturally occurring heteropolysaccharide, with large molecular weight, derived from the Gram-negative bacteria, Xanthomonas Campestris. This biopolymer has been studied extensively as a matrix for tablets, nanoparticles, microparticles, hydrogels, and various other formulation types. However, indigenous xanthan gum has its own set of restrictions, which may be overcome by chemical modification, to fine-tune the characteristics of the native gum, for attaining unmet demands. This approach has huge potential in the drug delivery and numerous other promising applications. The objective of this review is to provide a consolidated source of information on xanthan gum-based gastroretentive systems. Several approaches of floating techniques, with recent research avenues and patents, utilizing the natural polysaccharide xanthan gum is also discussed.
    MeSH terms: Animals; Drug Carriers/chemistry; Drug Compounding/methods; Humans; Drug Delivery Systems; Xanthomonas campestris
  3. Nordin N, Zaini Ambia NFA, Majid SR, Abu Bakar N
    Carbohydr Polym, 2025 Jan 15;348(Pt A):122830.
    PMID: 39562104 DOI: 10.1016/j.carbpol.2024.122830
    This study investigates the preliminary efficacy of drug encapsulation in chitosan hydrogels by cathodic electrodeposition for the encapsulation of the aromatic dye methyl orange to enhance drug delivery in biological systems. Chitosan, a biocompatible and transparent polymer, is known for its ability to effectively encapsulate and transport therapeutic agents, which is critical for sustained and targeted drug release. Methyl orange was selected as a model drug to study the effects of deposition and immersion times on encapsulation efficiency. The effects of deposition and immersion times on encapsulation efficiency were analyzed by synthesizing multilayer hydrogels via electrochemical oxidation. Characterization techniques, including UV-visible spectroscopy, FTIR, and NMR, were employed; FTIR indicated an effective absorption of 4.34 % for Td50Ti60, while UV-Vis showed 46.41 % at Td60Ti50. NMR analysis revealed effective concentrations of 0.47 mM for Td70Ti60 and 0.38 mM for Td60Ti50, indicating that longer immersion times enhance absorption. These findings provide a foundation for further studies aimed at optimizing drug delivery strategies and improving the therapeutic efficacy of encapsulated agents in biological applications.
    MeSH terms: Drug Carriers/chemistry; Drug Compounding/methods; Electrodes; Electroplating/methods; Spectrophotometry, Ultraviolet; Spectroscopy, Fourier Transform Infrared/methods; Hydrogels/chemistry; Drug Liberation
  4. Hua S, Shah SA, Nsang GEO, Sayyar R, Ullah B, Ullah N, et al.
    J Colloid Interface Sci, 2025 Feb;679(Pt A):487-495.
    PMID: 39374558 DOI: 10.1016/j.jcis.2024.09.219
    The development of cost-effective, highly active, and stable electrocatalysts for water splitting to produce green hydrogen is crucial for advancing clean and sustainable energy technologies. Herein, we present an innovative in-situ synthesis of FeOOH nanorods@NiOOH nanosheets on nickel foam (FeOOH@NiOOH/NF) at an unprecedentedly low temperature, resulting in a highly efficient electrocatalyst for overall water splitting. The optimized FeOOH@NiOOH/NF sample, evaluated through time-dependent studies, exhibits exceptional oxygen evolution reaction (OER) performance with a low overpotential of 261 mV at a current density of 20 mA cm-2, alongside outstanding hydrogen evolution reaction (HER) activity with an overpotential of 150 mV at a current density of 10 mA cm-2, demonstrating excellent stability in alkaline solution. The water-splitting device featuring FeOOH@NiOOH/NF-2 electrodes achieves a voltage of 1.59 V at a current density of 10 mA cm-2, rivalling the state-of-the-art RuO2/NF||PtC/NF electrode system. Density functional theory (DFT) calculations unveil the efficient functionality of the Fe sites within the FeOOH@NiOOH heterojunction as the active OER catalyst, while the Ni centres are identified as the active HER sites. The enhanced performance of OER and HER is attributed to the tailored electronic structure at the heterojunction, modified magnetic moments of active sites, and increased electron density in the dx2-y2 orbital of Fe. This work provides critical insights into the rational design of advanced electrocatalysts for efficient water splitting.
  5. Athul S, Kuttippurath J, Patel VK
    J Hazard Mater, 2025 Jan 05;481:136482.
    PMID: 39556909 DOI: 10.1016/j.jhazmat.2024.136482
    Severe restrictions on human travel and consumption during the lockdown (LD) have affected global marine traffic and operations. The LD period is ideal for studying the emissions as there were restricted human activities. Although several pollutants are emitted by ships, the most important is nitrogen dioxide (NO2), and can be considered an indicator of shipping emissions. Therefore, we examine the changes in NO2 pollution over the shipping lanes, ports and coasts across the globe during LD. Here, we find a significant decline in NO2 during LD over the major lanes, including the USA-Europe trade routes through the North Atlantic Ocean, Asia-Middle East through the Arabian Sea, Interasia, and Intereuropean through the North Sea and Baltic Sea, about 10-20%, as analysed form the TROPOMI satellite measurements. A similar reduction over the sea straight pass, such as the Cape of Good Hope and the Strait of Malacca is also estimated. Furthermore, the major global ports of Callao, Santos, Antwerp, Rotterdam, Busan, Tubarao, Richards Bay, Barcelona, Durban and Chennai exhibit a significant decrease in NO2 during LD, about 30%. The decline in NO2 over the shipping routes and ports can be attributed to reduced cargo, passenger, fishing and tanker vessel density due to the LD restrictions; consistent with the emission inventory analysis. Henceforth, this study suggests strict environmental policies in the shipping sector to curb emissions, as pollution is a great concern for public health in the port cities and coastal regions.
    MeSH terms: Air Pollutants/analysis; Air Pollution/analysis; Environmental Monitoring; Humans; Quarantine; Ships*
  6. Yappalparvi A, Balaraman AK, Padmapriya G, Gaidhane S, Kaur I, Lal M, et al.
    Respir Med, 2025 Jan;236:107863.
    PMID: 39557208 DOI: 10.1016/j.rmed.2024.107863
    BACKGROUND: Chronic obstructive pulmonary disease (COPD) significantly impacts global health due to persistent airflow limitation and inflammation. Despite standard therapies, symptoms persist. Ensifentrine, targeting both bronchoconstriction and inflammation as a dual phosphodiesterase 3 and 4 inhibitor, offers a promising therapeutic advancement for COPD management. This meta-analysis evaluates the safety and efficacy of ensifentrine in improving lung function, dyspnea, and quality of life in COPD patients.

    METHODS: We searched PubMed, Embase, and Web of Science through August 2024 for randomized controlled trials evaluating ensifentrine in COPD patients over a minimum of four weeks. Data extraction and screening utilized Knowledge software, and meta-analyses were performed using R v4.4 with a random-effects model.

    RESULTS: From 206 studies identified, four met our inclusion criteria. Ensifentrine improved FEV1 significantly at a dose of 3 mg (LS mean difference: 40.90 mL; 95 % CI: 19.65-62.15). It also improved dyspnea as measured by the Transition Dyspnea Index (TDI) (LS mean difference: 0.91; 95 % CI: 0.61-1.21) and quality of life according to the St. George's Respiratory Questionnaire-C (SGRQ-C) scores (LS mean difference: -1.92; 95 % CI: -3.28 to -0.55). Safety profiles were comparable between the ensifentrine and placebo groups, with no significant increase in treatment-emergent adverse events (TEAEs) (RR: 1.02; 95 % CI: 0.94-1.10).

    CONCLUSION: Ensifentrine significantly enhances lung function, reduces dyspnea, and improves quality of life in COPD patients, especially at a 3 mg dose. These benefits, coupled with a stable safety profile, support its use as an adjunctive therapy in COPD management.

    MeSH terms: Bronchodilator Agents/adverse effects; Bronchodilator Agents/therapeutic use; Forced Expiratory Volume/drug effects; Humans; Isoquinolines; Pyrimidinones; Quality of Life*; Randomized Controlled Trials as Topic; Treatment Outcome
  7. Deng X, Yang Z, Han M, Ismail N, Esa NM, Razis AFA, et al.
    Phytother Res, 2025 Jan;39(1):413-452.
    PMID: 39557422 DOI: 10.1002/ptr.8378
    Despite the advancement in cancer diagnosis and treatment, colorectal cancer remains the leading cause of cancer-related death worldwide. Given the high recurrence rate of colorectal cancer even after surgical resection, chemotherapy has been clinically used to improve the treatment outcomes of colorectal cancer. However, chemotherapy is well-known for its toxic side effects. Thus, phytochemicals have been widely studied in recent years as preventive and therapeutic agents for colorectal cancer owing to their relatively low toxicity. Moreover, combinatorial uses of phytochemicals with other natural compounds or with drugs may amplify the positive outcomes of colorectal cancer prevention and treatment by intervening in multiple signaling pathways and targets. This review summarized the combinatorial use of several well-studied groups of phytochemicals, that is, isothiocyanates, quinones, carotenoids, and alkaloids, in the prevention and treatment of colorectal cancer, and suggested it as a potential approach to improve the anticancer efficacy of single compounds and minimize the toxic side effects associated with conventional drugs. Notably, we generalized the in vitro, in vivo, and clinical experiments-based molecular mechanisms whereby the selected phytochemicals in combination with other compounds exerted anti-colorectal cancer effects by inhibiting cancer cell proliferation, cell apoptosis, cell invasion, and tumor growth. Overall, this review provides a reference and new perspective to propel further advancements in research and development of preventative and therapeutic strategies for colorectal cancer.
    MeSH terms: Animals; Antineoplastic Agents, Phytogenic/pharmacology; Antineoplastic Agents, Phytogenic/therapeutic use; Humans; Apoptosis/drug effects; Cell Proliferation/drug effects
  8. Sanggar A, Razak AA, Phoa PKA
    Psychogeriatrics, 2025 Jan;25(1):e13218.
    PMID: 39557512 DOI: 10.1111/psyg.13218
    BACKGROUND: Stressful life events greatly increase the risk of depression, which affects about 22% of older persons in northeast Malaysia. But how emotional distress manifests itself in reaction to these stressful events differs from person to person. Therefore, this study aims to delve into the nuances of emotional expression among older adults with depression symptoms in northeast Malaysia, alongside their help-seeking behaviours and utilisation of professional support.

    METHODS: Using a qualitative interpretative phenomenological approach, we conducted in-depth face-to-face interviews with participants, guided by Kleinman's explanatory model of illness. Nineteen older adults who screened positive for depression were included in the research.

    RESULTS: None of the participants explicitly acknowledged experiencing depression. However, they articulated their distress through three primary themes: 'Life is miserable,' 'Depression is a sign of weakness,' and 'Belief in pre-determination.' Remarkably, despite screening positive for depression, participants demonstrated a lack of awareness regarding available professional mental health services. Moreover, they expressed a reluctance to seek such services, citing reasons related to stigma and misconceptions. The predominant themes that emerged concerning help-seeking behaviours were 'Self-efficacy,' 'Social support,' and 'Formal assistance from non-mental healthcare professionals.'

    CONCLUSION: The expression of emotion among older adults is restricted by socio-cultural influences. Thus, there is a need to improve mental health literacy among older adults in Malaysia, and their preferred source of support such as religious leaders and non-mental healthcare physicians.

    MeSH terms: Help-Seeking Behavior*; Aged; Aged, 80 and over; Female; Humans; Interviews as Topic; Malaysia; Male; Mental Health Services/statistics & numerical data; Middle Aged; Patient Acceptance of Health Care/psychology; Social Support*; Expressed Emotion*; Qualitative Research*; Social Stigma
  9. Al-Shajrawi OM, Tarawneh IAM, Tengku Din TADAADAA, Afolabi HA
    Mol Clin Oncol, 2025 Jan;22(1):6.
    PMID: 39559458 DOI: 10.3892/mco.2024.2801
    Cancer is one of the deadliest health menaces humans have ever witnessed. It is a leading cause of human mortality. Today, it remains a main leading cause of death globally primarily due to lifestyle changes and population ageing. A total of ~12.7 million cancer cases and 7.6 million cancer deaths were reported in 2008. In developing countries, cancer accounted for 56% of cases and 64% of deaths. Tamoxifen is the most reputable and recommended specific oestrogen receptor modulator drug used for the treatment of breast cancer. In the past decade, algae have demonstrated remarkable potency for advanced life applications. They can remain a focus of interest in the coming decades because they are one of the most diverse organisms in the entire ecosystem with immense bio nutritional benefits. Algae and their extracts play a pivotal role in the pharmaceutical industry as bioactive compounds and new drugs and nutraceutical industry as probiotics and antioxidants. However, a broad range of the health benefits of these organisms remains to be explored. The present review highlights the applications and co-application of microalgal crude extracts with tamoxifen for breast cancer immunotherapy. Given that recent studies have suggested that tamoxifen is an essential and primary treatment for breast cancer, the present review focused on the identification of a new treatment approach involving the co-application of tamoxifen and microalgal extracts to provide promising anticancer activity with few side effects on normal cells. The present review includes a general background and blueprint for the use of microalgal extracts as potential and affordable treatments or adjuncts for breast cancer management.
  10. Lu X, Teh SY, Tay CJ, Abu Kassim NF, Fam PS, Soewono E
    Infect Dis Model, 2025 Mar;10(1):240-256.
    PMID: 39559512 DOI: 10.1016/j.idm.2024.10.007
    Despite the implementation of various initiatives, dengue remains a significant public health concern in Malaysia. Given that dengue has no specific treatment, dengue prediction remains a useful early warning mechanism for timely and effective deployment of public health preventative measures. This study aims to develop a comprehensive approach for forecasting dengue cases in Selangor, Malaysia by incorporating climate variables. An ensemble of Multiple Linear Regression (MLR) model, Long Short-Term Memory (LSTM), and Susceptible-Infected mosquito vectors, Susceptible-Infected-Recovered human hosts (SI-SIR) model were used to establish a relation between climate variables (temperature, humidity, precipitation) and mosquito biting rate. Dengue incidence subject to climate variability can then be projected by SI-SIR model using the forecasted mosquito biting rate. The proposed approach outperformed three alternative approaches and expanded the temporal horizon of dengue prediction for Selangor with the ability to forecast approximately 60 weeks ahead with a Mean Absolute Percentage Error (MAPE) of 13.97 for the chosen prediction window before the implementation of the Movement Control Order (MCO) in Malaysia. Extended validation across subsequent periods also indicates relatively satisfactory forecasting performance (with MAPE ranging from 13.12 to 17.09). This research contributed to the field by introducing a novel framework for the prediction of dengue cases over an extended temporal range.
  11. Jusril NA, Mohd KS, Abd Mutalib N, Mohd Badiazaman AA
    Biomed Rep, 2025 Jan;22(1):9.
    PMID: 39559820 DOI: 10.3892/br.2024.1887
    Propolis is a sticky substance produced by stingless bees for construction and defence of their hive. It has notable anti-inflammatory, antioxidant, antibacterial, antifungal, anti-hyperglycemic, and wound healing effects. The present review summarised and examined the phytochemical properties, mode of action and current research prospects of Malaysian propolis. A database search using Google Scholar, Web of Science and ScienceDirect generated 780 references; 30 relevant articles were included in the present review, of which 23 were in vitro studies and 7 were in vivo or animal studies. Propolis demonstrated antioxidant, antibacterial, antifungal, anti-inflammatory and anti-hyperglycemic properties, indicating potential as a wound healing agent. Despite favourable findings, due to the scarcity of studies in the literature, more in-depth research and clinical validation on the synergistic effects, efficacy and optimum dosage of propolis are needed.
  12. Lyu FF, Ramoo V, Chui PL, Ng CG
    West J Nurs Res, 2025 Jan;47(1):33-46.
    PMID: 39559966 DOI: 10.1177/01939459241301295
    OBJECTIVE: This study aimed to adapt, validate, and evaluate the feasibility of a mindful exercise program (Mindful Standing Yoga combined with Baduanjin) for older patients with primary osteoporosis.

    METHODS: The study was conducted in 3 stages. Stage 1 involved a qualitative focus group discussion with 6 experts to gather perspectives on modifying the exercise program. Stage 2 used a Delphi approach with another 6 experts to validate the program. In Stage 3, a feasibility study was conducted with 20 eligible patients (out of 23 initially enrolled) at a traditional Chinese medicine hospital, using a single-group pre- and posttest design. The strenuousness of the adapted exercise was assessed through heart rate (HR) and rating of perceived exertion (RPE). Its effects were measured using the Global Pain Scale (GPS), the Five Facets Mindfulness Questionnaire-Short Form (FFMQ-SF), the Tampa Kinesiophobia-11 Scale, and the Timed Up and Go test. Patient satisfaction and feedback were also collected.

    RESULTS: The modified program, consisting of 9 movements and taking 30 min to complete, was validated by experts as suitable, safe, and effective for practice. HR and RPE measurements confirmed it as a low-intensity exercise and not strenuous for the study population. The program significantly improved back pain and mindfulness in the feasibility study, with most participants expressing satisfaction with the protocol.

    CONCLUSION: Experts and participants affirmed that the program was appropriate and satisfactory for older patients with primary osteoporosis, particularly those with back pain. Further high-quality randomized controlled trials are needed to validate its effectiveness.

    MeSH terms: Aged; Delphi Technique; Exercise Therapy/methods; Feasibility Studies*; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Mindfulness/methods
  13. Siew SW, Khairi MHF, Hamid NA, Asras MFF, Ahmad HF
    Environ Pollut, 2025 Jan 01;364(Pt 1):125330.
    PMID: 39551377 DOI: 10.1016/j.envpol.2024.125330
    The burgeoning crises of antimicrobial resistance and plastic pollution are converging in healthcare settings, presenting a complex challenge to global health. This study investigates the microbial populations in healthcare waste to understand the extent of antimicrobial resistance and the potential for plastic degradation by bacteria. Our metagenomic analysis, using both amplicon and shallow shotgun sequencing, provided a comprehensive view of the taxonomic diversity and functional capacity of the microbial consortia. The viable bacteria in healthcare waste samples were analyzed employing full-length 16S rRNA sequencing, revealing a diverse bacterial community dominated by Firmicutes and Proteobacteria phyla. Notably, Proteus mirabilis VFC3/3 and Pseudomonas sp. VFA2/3 were detected, while Stenotrophomonas maltophilia VFV3/2 surfaced as the predominant species, holding implications for the spread of hospital-acquired infections and antimicrobial resistance. Antibiotic susceptibility testing identified multidrug-resistant strains conferring antimicrobial genes, including the broad-spectrum antibiotic carbapenem, underscoring the critical need for improved waste management and infection control measures. Remarkably, we found genes linked to the breakdown of plastic that encoded for enzymes of the esterase, depolymerase, and oxidoreductase classes. This suggests that specific bacteria found in medical waste may be able to reduce the amount of plastic pollution that comes from biological and medical waste. The information is helpful in formulating strategies to counter the combined problems of environmental pollution and antibiotic resistance. This study emphasises the importance of monitoring microbial communities in hospital waste in order to influence waste management procedures and public health policy. The findings highlight the need for a multidisciplinary approach to mitigate the risks associated with antimicrobial resistance and plastic waste, especially in hospital settings where they intersect most acutely.
    MeSH terms: Anti-Bacterial Agents/pharmacology; Drug Resistance, Microbial/genetics; Medical Waste; Plastics*; RNA, Ribosomal, 16S/genetics; Drug Resistance, Bacterial/genetics
  14. Liu H, Zou M, Shen M, Kamarulzaman A, Chen S, Li J, et al.
    Int J Cancer, 2025 Mar 15;156(6):1225-1235.
    PMID: 39551969 DOI: 10.1002/ijc.35242
    The presence of human immunodeficiency virus (HIV) infection increases the risk of acquiring human papillomavirus (HPV) infection and developing HPV-related adversities. We aimed to estimate the cost-effectiveness of HPV vaccination for women living with HIV in a Chinese setting. A decision-analysis Markov model was developed to estimate the cost-effectiveness of 36 HPV vaccination strategies for women living with HIV aged 18-45 years, from the healthcare system perspective. With the status quo, not vaccinating women living with HIV would lead to 51.99% (51,985/100,000) HIV-related deaths; 35.10% (35,098/100,000) would develop genital warts, 0.36% (355/100,000) develop cervical cancer, and among which 63.66% (226/355) die from cervical cancer over their lifetime (1,601,457 person-years). With a willingness to pay (WTP) threshold of three times gross domestic product (GDP), Gardasil 4 vaccination for all women living with HIV aged 18-45 years was the most cost-effective strategy (ICER = US $32,766/QALY gained). This strategy would reduce genital warts by 35.52% (12,467/35,098), cervical cancers by 12.96% (46/355), and cervical cancer deaths by 12.39% (28/226) over the lifetime of the cohort. If the future domestic Cecolin 9 vaccine is priced at 60% of Gardasil 9, vaccinating all women living with HIV aged 18-45 years with Cecolin 9 would be the most cost-effective strategy (ICER = US $30,493/QALY gained). Improving adherence to antiretroviral therapy for HIV may substantially improve the cost-effectiveness of both Gardasil 4 and Cecolin 9 vaccination.
    MeSH terms: Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18/administration & dosage; Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18/economics; Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18/therapeutic use; Adolescent; Adult; China/epidemiology; Cost-Benefit Analysis*; Female; Humans; Markov Chains; Middle Aged; Vaccination/economics; Vaccination/methods; Quality-Adjusted Life Years; Young Adult
  15. Ching KWC, Mokhtar NF, Tye GJ
    Comput Biol Med, 2025 Jan;184:109396.
    PMID: 39549529 DOI: 10.1016/j.compbiomed.2024.109396
    Metastatic breast cancer (MBC) is an advanced-stage breast cancer associated with more than 90 % of cancer-related deaths. Immunosuppressive properties of tolerogenic dendritic cells (tolDCs) in tumour immune microenvironment (TIME) may be a risk factor for the rapid progression to MBC. However, the exact connections between the two are unknown. The aim of the current study is to uncover gene signatures and key pathways associated with MBC and tolDCs via an integrated bioinformatics approach. Gene expression profiles of MBC and tolDCs were retrieved from Gene Expression Omnibus (GEO) to identify common differentially expressed genes (DEGs). From DGE analysis, 529 upregulated common DEGs and 367 downregulated common DEGs had been identified. In enrichment analysis, common DEGs enriched in GO terms of defense response to virus and KEGG pathway of transcriptional misregulation in cancer were reported to be significantly associated with MBC and tolDCs. From the constructed PPI networks, 23 hub genes were identified, although only 5 genes were significant; 3 upregulated (ISG15, OAS2 and RSAD2) and 2 downregulated (eEF2 and PPARG) as they were found to be significantly correlated and had the same expression trend as predicted in validation analysis of overall survival (OS) analysis, expression levels, immune infiltration analysis and immunohistochemistry (IHC) analysis. These 5 hub genes can now be exploited in developing novel therapeutic interventions and as diagnostic biomarkers for enhancing the clinical outcomes of MBC patients.
    MeSH terms: Female; Humans; Neoplasm Metastasis; Biomarkers, Tumor/genetics; Gene Expression Regulation, Neoplastic*; Computational Biology*; Gene Expression Profiling; Databases, Genetic; Gene Regulatory Networks; Tumor Microenvironment/genetics; Tumor Microenvironment/immunology; Transcriptome/genetics; Protein Interaction Maps/genetics
  16. Lee DE, Husain A, Khan A, Danish M, Jo WK
    Environ Res, 2025 Jan 01;264(Pt 1):120367.
    PMID: 39549909 DOI: 10.1016/j.envres.2024.120367
    Integrating photocatalysis with electrocatalysis may represent a synergistic approach to address environmental and energy challenges. In this context, we explored synthesizing a series of nanocomposite materials using a solid-state approach involving simple grinding and subsequent thermal treatment for the photocatalytic purification of dinoseb and electrocatalytic oxygen evolution (OER). Interestingly, among the series of synthesized materials, 40 wt percentage of 3D/2D/1D:ZnFe2O4/NiAl-LDH/MWCNTs ternary nanocomposite (40-NZM) showed highly improved dinoseb detoxification and OER efficiencies compared to those of pure materials. Importantly, approximately 98% detoxification of dinoseb was observed within 75 min of irradiation time under a visible light source. Remarkably, the 40-NZM nanocomposite exhibited the highest rate constant value (k = 4.1 × 10-2 min-1) with a favorable R2 (0.98) parameter. Furthermore, 40-NZM showed promising electrocatalytic OER performance, requiring only 217 mV of overpotential to achieve 10 mAcm-2 of current density with a smaller Tafel slope of 66.6 mVdec-1. Additionally, long-term stability was tested by recording 2000 cyclic voltammetry (CV) cycles. The results revealed that 40-NZM could maintain its catalytic activity for a longer duration as it required only 227 mV to attain 10 mAcm-2 even after 2000 CV cycles. Consequently, these outstanding characteristics of 40-NZM nanocomposite underscore the significant potential for catalytic water purification and sustainable energy conversion.
    MeSH terms: Catalysis; Ferric Compounds/chemistry; Electrochemical Techniques/methods; Photochemical Processes
  17. Tunkl C, Agarwal A, Ramage E, Velez FS, Roushdy T, Ullberg T, et al.
    Int J Stroke, 2025 Mar;20(3):297-309.
    PMID: 39460528 DOI: 10.1177/17474930241298450
    BACKGROUND: Despite the proven efficacy of telestroke in improving clinical outcomes by providing access to specialized expertise and allowing rapid expert hyperacute stroke management and decision-making, detailed operational evidence is scarce, especially for less developed or lower income regions.

    AIM: We aimed to map the global telestroke landscape and characterize existing networks.

    METHODS: We employed a four-tiered approach to comprehensively identify telestroke networks, primarily involving engagement with national stroke experts, stroke societies, and international stroke authorities. A carefully designed questionnaire was then distributed to the leaders of all identified networks to assess these networks' structures, processes, and outcomes.

    RESULTS: We identified 254 telestroke networks distributed across 67 countries. High-income countries (HICs) concentrated 175 (69%) of the networks. No evidence of telestroke services was found in 58 (30%) countries. From the identified networks, 88 (34%) completed the survey, being 61 (71%) located in HICs. Network setup was highly heterogeneous, ranging from 17 (22%) networks with more than 20 affiliated hospitals, providing thousands of annual consultations using purpose-built highly specialized technology, to 11 (13%) networks with fewer than 120 consultations annually using generic videoconferencing equipment. Real-time video and image transfer was employed in 64 (75%) networks, while 62 (74%) conducting quality monitoring. Most networks established in the past 3 years were located in low- and middle-income countries (LMICs).

    CONCLUSION: This comprehensive global survey of telestroke networks found significant variation in network coverage, setup, and technology use. Most services are in HICs, and a few services are in LMICs, although an emerging trend of new networks in these regions marks a pivotal moment in global telestroke care. The wide variation in quality monitoring practices across networks, with many failing to report key performance metrics, underscores the urgent need for standardized, resource-appropriate, quality assurance measures that can be adapted to diverse settings.

    MeSH terms: Developing Countries; Humans; Surveys and Questionnaires; Global Health; Telemedicine*
  18. Wong WJ, Nguyen TV, Ahmad F, Vu HTT, Koh AS, Tan KM, et al.
    J Clin Hypertens (Greenwich), 2025 Jan;27(1):e14936.
    PMID: 39545715 DOI: 10.1111/jch.14936
    Diabetes is one of the most pressing health issues in the Southeast Asian region, and hypertension has been commonly reported as a comorbidity in adults with diabetes. This systematic review aimed to synthesize evidence on the prevalence and management of hypertension in adults with diabetes in Southeast Asian countries. A literature search was conducted in Ovid MEDLINE and Embase Classic + Embase from database inception until March 15, 2024. Studies were included if (1) they were conducted in Southeast Asian countries, (2) the study populations were adults with diabetes, and (3) there was information related to hypertension or blood pressure (BP) in the study results. Of the 7486 abstracts found, 90 studies qualified for this review. Most studies reported a hypertension prevalence of 70% or higher (ranging from 29.4% to 93.4%). Despite this high prevalence, a substantial proportion of these populations did not receive adequate BP control, with most studies indicating a control rate of less than 40%. There was limited evidence on the prescription of antihypertensive therapies and medication adherence. There was a lack of studies from 4 of the 11 countries in the region. This review highlights that BP control in adults with diabetes remains a significant challenge in Southeast Asia. Given the ongoing epidemiological transition, and the increasing older population in this region who are likely to accumulate multiple chronic conditions complicating medication strategies, this review highlights the urgent need to improve BP management in those with diabetes.
    MeSH terms: Adult; Aged; Asia, Southeastern/epidemiology; Blood Pressure/drug effects; Blood Pressure/physiology; Diabetes Mellitus/epidemiology; Female; Humans; Male; Middle Aged; Comorbidity; Prevalence; Medication Adherence
  19. Selim HMRM, Gomaa FAM, Alshahrani MY, Morgan RN, Aboshanab KM
    Ther Deliv, 2025 Mar;16(3):247-269.
    PMID: 39545771 DOI: 10.1080/20415990.2024.2426824
    The ongoing global health crisis caused by multidrug-resistant (MDR) bacteria necessitates quick interventions to introduce new management strategies for MDR-associated infections and antimicrobial agents' resistance. Phage therapy emerges as an antibiotic substitute for its high specificity, efficacy, and safety profiles in treating MDR-associated infections. Various in vitro and in vivo studies denoted their eminent bactericidal and anti-biofilm potential. This review addresses the latest developments in phage therapy regarding their attack strategies, formulations, and administration routes. It additionally discusses and elaborates on the status of phage therapy undergoing clinical trials, and the challenges encountered in their usage, and explores prospects in phage therapy research and application.
    MeSH terms: Animals; Anti-Bacterial Agents/administration & dosage; Bacteria/drug effects; Bacteriophages*; Clinical Trials as Topic*; Humans; Biofilms/drug effects; Drug Resistance, Multiple, Bacterial*
  20. Palani R, Wu YS, Wu SH, Chang JK, Jose R, Yang CC
    J Colloid Interface Sci, 2025 Feb 15;680(Pt A):845-858.
    PMID: 39546905 DOI: 10.1016/j.jcis.2024.11.066
    Rechargeable lithium-oxygen (Li-O2) batteries are being considered as the next-generation energy storage systems due to their higher theoretical energy density. However, the practical application of Li-O2 batteries is hindered by slow kinetics and the formation of side products during the oxygen reduction and evolution reactions on the cathode. These reactions lead to high overpotentials during charging and discharging. To address these challenges, we propose a simple ultrasonic method for synthesizing cobalt nanoclusters embedded in nitrogen-doped graphene nanosheets (GrZnCo) derived from metal-organic frameworks (MOFs). The resulting material, due to the retention of metallic cobalt structure, exhibits better electronic conductivity. Additionally, the GrZnCo catalyst shows vigorous catalytic activity, which can improve reaction kinetics and suppress side reactions, thus lowering the charging overpotential. We have investigated the impact of different catalyst compositions (GrZnCox; x  = 1, 3, 5) by varying the amounts of cobalt and zinc. The optimum catalyst, GrZnCo3, contains high cobalt-N active components, graphitic-N, pyridinic-N, pyrrolic-N, and abundant defect structures, which enhance the electrochemical performance. The defect-rich GrZnCo3 catalyst enables Li-O2 batteries to achieve a high discharge capacity of 13500 mAh·g-1 at 50 mA·g-1 and a remarkable long-term cycling performance of over 400 cycles at 100 mA·g-1 with a limited capacity of 500 mAh·g-1. This work demonstrates an effective approach to fabricate cost-effective electrocatalysts for various energy storage systems.
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